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2. Mating harassment may boost the effectiveness of the sterile insect technique for Aedes mosquitoes

Author

Zhang, Dongjing, Maiga, Hamidou, Li, Yongjun, Bakhoum, Mame Thierno, Wang, Gang, Sun, Yan, Damiens, David, Mamai, Wadaka, Bimbilé Somda, Nanwintoum Séverin, Wallner, Thomas, Bueno-Masso, Odet, Martina, Claudia, Kotla, Simran Singh, Yamada, Hanano, Lu, Deng, Tan, Cheong Huat, Guo, Jiatian, Feng, Qingdeng, Zhang, Junyan, Zhao, Xufei, Paerhande, Dilinuer, Pan, Wenjie, Wu, Yu, Zheng, Xiaoying, Wu, Zhongdao, Xi, Zhiyong, Vreysen, Marc J. B., and Bouyer, Jérémy

Published
2024
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5. Mating harassment may boost the effectiveness of the sterile insect technique for Aedes mosquitoes

Author

Bouyer, Jérémy, primary, Zhang, Dongjing, additional, Maiga, Hamidou, additional, Li, Yongjun, additional, Bakhoum, Mame Thierno, additional, Wang, Gang, additional, Sun, Yan, additional, Damiens, David, additional, Mamai, Wadaka, additional, Somda, Nanwintoum, additional, Wallner, Thomas, additional, Masso, Odet Bueno, additional, Martina, Claudia, additional, Kotla, Simran, additional, Yamada, Hanano, additional, Deng, Lu, additional, Tan, Cheong Huat, additional, Guo, Jiatian, additional, Feng, Qingdeng, additional, Zhang, Junyan, additional, Zhao, Xufei, additional, Paerhande, Dilinuer, additional, Pan, Wenjie, additional, Wu, Yu, additional, Zheng, Xiaoying, additional, Wu, Zhongdao, additional, Xi, Zhiyong, additional, and Vreysen, Marc, additional

Published
2023
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9. Could Sterile Aedes albopictus Male Releases Interfere with Aedes aegypti Population in Reunion Island?

Author

Andrianjakarivony, Harilanto Felana, Damiens, David, Marquereau, Lucie, Gaudillat, Benjamin, Habchi-Hanriot, Nausicaa, Gouagna, Louis-Clément, COMBE, Isabelle, Microbes évolution phylogénie et infections (MEPHI), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institut méditerranéen d'océanologie (MIO), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM), and GIP-CYROI, Inserm U1187, CNRS 9192, University of Reunion Island

Subjects
[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, [SDV.MHEP.ME] Life Sciences [q-bio]/Human health and pathology/Emerging diseases, [SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases, fungi, virus diseases, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, [SDV.MHEP.CSC] Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, [SDV.MP.PAR] Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology
Abstract

International audience; In Reunion Island, the feasibility of an Aedes albopictus control program using the Sterile Insect Technique (SIT) is studied. Because, in some regions, Ae. albopictus is living in sympatry with Aedes aegypti, the impact of releasing millions of sterile male Ae. albopictus on female Ae. aegypti reproduction needs to be assessed. Thus, to study the potential heterospecific matings, a marking technique using rhodamine B has been used. Rhodamine is given in solution to male mosquitoes to be incorporated into the male body and seminal fluid and transferred during mating into the bursa inseminalis and spermathecae of females. The presence of rhodamine in females occurred in 15% of cases when Ae. aegypti females were offered non-irradiated Ae. albopictus males, 5% when offered irradiated Ae. albopictus males and 18% of cases in the inverse heterospecific matings. Moreover, our results also showed that these matings gave few eggs but were not viable. Finally, the results showed that whatever the type of mating crosses, females in cages previously crossed with males of another species can re-mate with males of their species and produce an equivalent amount of egg compared to females only mated with conspecific males. Despite the promiscuity of the males and females in small cages for three days, heterospecific mating between sterile male Ae. albopictus and female Ae aegypti, 95% of the females have not been inseminated suggesting that in the field the frequency satyrization would be very low.

Published
2022

12. Additional file 2 of Estimates of the population size and dispersal range of Anopheles arabiensis in Northern KwaZulu-Natal, South Africa: implications for a planned pilot programme to release sterile male mosquitoes

Author

Kaiser, Maria L., Wood, Oliver R., Damiens, David, Brooke, Basil D., Koekemoer, Lizette L., and Munhenga, Givemore

Abstract

Additional file 2. Sweep net design. A Sweep net. Net length: ~ 1.5 m, hoop diameter: ~ 70 cm, handle: ~ 2 m. B Mid notch and lashing. C Top notch and lashing.

Published
2021
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14. Complementarity of empirical and process-based approaches to modelling mosquito population dynamics with Aedes albopictus as an example

Author

Tran, Annelise, Mangeas, Morgan, Demarchi, Marie, Roux, Emmanuel, Degenne, Pascal, Haramboure, Marion, Le Goff, Gilbert, Damiens, David, Gouagna, Louis-Clément, Herbreteau, Vincent, Dehecq, Jean-Sébastien, Territoires, Environnement, Télédétection et Information Spatiale (UMR TETIS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département Environnements et Sociétés (Cirad-ES), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), UMR 228 Espace-Dev, Espace pour le développement, Institut de Recherche pour le Développement (IRD)-Université de Perpignan Via Domitia (UPVD)-Avignon Université (AU)-Université de La Réunion (UR)-Université de Montpellier (UM)-Université de Guyane (UG)-Université des Antilles (UA), Recherche en épidémiologie et biostatistique, Université Paris-Sud - Paris 11 (UP11)-Institut National de la Santé et de la Recherche Médicale (INSERM), Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département Systèmes Biologiques (Cirad-BIOS), Diversity, ecology, evolution & Adaptation of arthropod vectors (MIVEGEC-DEEVA), Evolution des Systèmes Vectoriels (ESV), Génétique et évolution des maladies infectieuses (GEMI), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Génétique et évolution des maladies infectieuses (GEMI), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Agence Régionale de Santé Océan Indien, Agence Régionale de la Santé (ARS), This study was partially fundedby the Regional Health Agency in Reunion Island (https:// www.ocean-indien.ars.sante.fr/), and FEDER INTERREGTROI project (2015-2017) under the platform in partnership One Health Indian Ocean (www.onehealth-oi.org). MMreceived a grant from EuropeanResearch Council(https://erc.europa.eu): ERC Consolidatorgrant n˚682387 / REVOLINC project. JSD (Regional Health Agency,Reunion Island) provided part of the entomological dataset and approved the final manuscript, and Le Goff, Gilbert

Subjects
[SDV] Life Sciences [q-bio], [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, [SDV]Life Sciences [q-bio], [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
Abstract

International audience; Mosquitoes are responsible for the transmission of major pathogens worldwide. Modelling their population dynamics and mapping their distribution can contribute effectively to disease surveillance and control systems. Two main approaches are classically used to understand and predict mosquito abundance in space and time, namely empirical (or statistical) and process-based models. In this work, we used both approaches to model the population dynamics in Reunion Island of the 'Tiger mosquito', Aedes albopictus, a vector of dengue and chikungunya viruses, using rainfall and temperature data. We aimed to i) evaluate and compare the two types of models, and ii) develop an operational tool that could be used by public health authorities and vector control services. Our results showed that Ae. albopictus dynamics in Reunion Island are driven by both rainfall and temperature with a non-linear relationship. The predictions of the two approaches were consistent with the observed abundances of Ae. albopictus aquatic stages. An operational tool with a user-friendly interface was developed, allowing the creation of maps of Ae. albopictus densities over the whole territory using meteorological data collected from a network of weather stations. It is now routinely used by the services in charge of vector control in Reunion Island.

Published
2020

15. Modelling the control of Aedes albopictus mosquitoes based on sterile males release techniques in a tropical environment

Author

Haramboure, Marion, Labbé, Pierrick, Baldet, Thierry, Damiens, David, Gouagna, Louis Clément, Bouyer, Jérémy, Tran, Annelise, Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Insect Pest Control Laboratory (IPC laboratory), Food and Agriculture Organization of the United Nations [Rome, Italie] (FAO)-International Atomic Energy Agency [Vienna] (IAEA), Territoires, Environnement, Télédétection et Information Spatiale (UMR TETIS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département Environnements et Sociétés (Cirad-ES), European Project: 682387,REVOLINC, École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UR226, Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-AgroParisTech-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École pratique des hautes études (EPHE)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA), and Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)

Subjects
[SDV.BIO]Life Sciences [q-bio]/Biotechnology, Boosted SIT, Population dynamics, L73 - Maladies des animaux, Mosquito, Dynamique des populations, [MATH.MATH-AP]Mathematics [math]/Analysis of PDEs [math.AP], ComputingMilieux_MISCELLANEOUS, [SDE.IE]Environmental Sciences/Environmental Engineering, Contrôle de maladies, Lâcher d'insectes stériles, L70 - Sciences et hygiène vétérinaires - Considérations générales, Aedes albopictus, Vector control, SIT, Vecteur de maladie, Modélisation, Pyriproxyfen, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Zone tropicale
Abstract

International audience; The Sterile Insect Technique (SIT), used to control insect populations, consists of releasing males sterilized by ionizing radiations. Wild females that mate with these males can no longer produce viable offspring, which may drives the population decline. Although this method has proved its efficiency, its effect may be more limited for fast-reproducing large-population species, such Aedes albopictus. A novel approach, named ”boosted SIT” has been designed to strengthen the SIT technique: It consists of coating sterile males with a biocide that will be transferred to the mated females, which will then contaminate the oviposition sites. This study is aimed at exploring demographic effects of both techniques (SIT and boosted SIT) through their inclusion in a weather-driven abundance model of the Aedes albopictus population dynamics in the geographical context of La Reunion Island. Sensitivity analysis showed that the date to start the release, as well as the quantity of sterile males released and their competitiveness, are of key importance for both control methods. According to our results, boosted SIT allows 1) Increasing the effectiveness of the SIT when the sterile males released are of medium quality in terms of competitiveness, and 2) extending the optimal window to start the control period.

Published
2020

16. Strategic Approach, Advances, and Challenges in the Development and Application of the SIT for Area-Wide Control of Aedes albopictus Mosquitoes in Reunion Island

Author

Gouagna, Louis Clément, primary, Damiens, David, additional, Oliva, Clélia F., additional, Boyer, Sébastien, additional, Le Goff, Gilbert, additional, Brengues, Cécile, additional, Dehecq, Jean-Sébastien, additional, Raude, Jocelyn, additional, Simard, Frédéric, additional, and Fontenille, Didier, additional

Published
2020
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19. Laboratory evaluation of the effects of sterilizing doses of gamma-rays from Caesium-137 source on the daily flight activity and flight performance of Aedes albopictus males

Author

Lebon, C., Soupapoule, K., Wilkinson, D. A., Le Goff, Gilbert, Damiens, David, and Gouagna, Louis-Clément

Abstract

The control of Aedes albopictus through Sterile Male Releases requires that the most competitive males be mass-reared and sterilized usually with gamma- or X-ray radiation prior to release. Developing an understanding of the impact of irradiation treatment on flight performance in sterile males is very important because any fitness cost may reduce the efficacy of SIT intervention in the field. Here, we examined the role of irradiation exposure and sugar-feeding on daily flight activity and performance of Ae. albopictus males sterilized during pupal stage with gamma-radiation at 35Gray from a Caesium 137 source. We used a previously developed automated video tracking system to monitor the flight activity of different groups of sterile and control non-sterile males over 24 hours in a flight arena. This monitoring took place under controlled laboratory conditions and we wished to quantify the daily flight activity and to highlight any changes due to radiation treatment and nutritional conditions (starved versus sugar fed). Our experimental evidence demonstrated a characteristic diurnal flight activity with a bimodal pattern regardless of the treatment. Precisely, both irradiated and non-irradiated males exhibited two distinct peaks in flight activity in the morning (6-8 a.m.) and late afternoon (4-6 p.m.). Under changing physiological conditions, irradiated males were generally more active over time and flew longer overall distances than control male populations. These results suggest some internal circadian control of the phase relation to the light-dark cycle, with evidence for modification of flight performance by nutritional status. The fact that daily activity patterns are alike in irradiated and control Ae. albopictus males, and that sterile males could display higher flight performance, is in contrast with the hypothesis that irradiation treatment appears to reduce the fitness of male mosquitoes. We discuss the implications of the present study in sterile-male release programs against Ae. albopictus.

Published
2018

21. Bad housekeeping: why do aphids leave their exuviae inside the colony?

Author

Boivin Guy, Hance Thierry, Damiens David, and Muratori Frédéric B

Subjects
Evolution, QH359-425
Abstract

Abstract Background Animals can gain protection against predators and parasites by living in groups. The encounter-dilution effect provides protection when the probability of detection of a group does not increase in proportion to group size (i.e. encounter effect), so that predators do not offset the encounter effect by attacking more members of the group (i.e. dilution effect). In this paper, we propose a novel mechanism by which prey insects could gain by producing decoys that act as multiple targets for predators or parasitoids if these decoys are recognised as preys or hosts and negatively affect the patch foraging strategy of these predators and parasitoids. Such a decoy mechanism could be present in aphid colonies in which aphid exuviae are recognised and attacked by Aphidiine wasps. Results We conducted a behavioural study to evaluate the effect of exuviae on parasitoid patch residence time and egg allocation in experimental aphid patches with or without exuviae. We showed that exuviae are recognised and attacked at the same level as aphids when both are present in the patch. While parasitism rate was not significantly lower in patches with exuviae when the parasitoid left the patch, the time wasted by parasitoids to handle exuviae did influence the patch residence time. As a consequence, the attack rate on the live aphids was lower in patches that contain exuviae. Conclusion Aphids had more time available to flee and thus each individual might gain protection against parasitoids by leaving their exuviae near and within the colony. These results demonstrate that the encounter-dilution effect provided by living in a group can be enhanced by extra-materials that act as decoy for natural enemies.

Published
2008
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23. Enhancements to the mass-rearing cage for the malaria vector, Anopheles arabiensis for improved adult longevity and egg production

Author

Maïga, Hamidou, primary, Bimbilé-Somda, Nanwintoum Séverin, additional, Yamada, Hanano, additional, Wood, Oliver, additional, Damiens, David, additional, Mamai, Wadaka, additional, Balestrino, Fabrizio, additional, Lees, Rosemary S., additional, Dabiré, Roch K., additional, Diabaté, Abdoulaye, additional, and Gilles, J.R.L., additional

Published
2017
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26. Darwin’s finches treat their feathers with a natural repellent

Author

Cimadom, Arno, primary, Causton, Charlotte, additional, Cha, Dong H., additional, Damiens, David, additional, Fessl, Birgit, additional, Hood-Nowotny, Rebecca, additional, Lincango, Piedad, additional, Mieles, Alejandro E., additional, Nemeth, Erwin, additional, Semler, Elizabeth M., additional, Teale, Stephen A., additional, and Tebbich, Sabine, additional

Published
2016
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33. Mating competitiveness of sterile male Anopheles coluzzii in large cages

Author

Maïga, Hamidou, primary, Damiens, David, additional, Niang, Abdoulaye, additional, Sawadogo, Simon P, additional, Fatherhaman, Omnia, additional, Lees, Rosemary S, additional, Roux, Olivier, additional, Dabiré, Roch K, additional, Ouédraogo, Georges A, additional, Tripet, Fréderic, additional, Diabaté, Abdoulaye, additional, and Gilles, Jeremie RL, additional

Published
2014
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34. Comparison of Efficiency of BG-Sentinel Traps Baited with Mice, Mouse-Litter, and CO2 Lures for Field Sampling of Male and Female Aedes albopictus Mosquitoes.

Author

Le Goff, Gilbert, Damiens, David, Ruttee, Abdoul-Hamid, Payet, Laurent, Lebon, Cyrille, Dehecq, Jean-Sébastien, Geier, Martin, and Gouagna, Louis-Clément

Subjects
*AEDES albopictus, *MOSQUITO control, *INSECT baits & repellents, *INSECT traps, *BODY odor, MICE anatomy
Abstract

Determining the abundance and distribution of male mosquitoes in the wild and establishing species seasonality in candidate pilot sites is of particular interest with respect to the use of the sterile-male technique. With the knowledge that using mice as bait in BG-Sentinel traps effectively enhances Aedes albopictus male and female trapping success, the present study was designed to determine whether attractants derived from mouse odour blend could be a viable substitute for live mice to lure Ae. albopictus mosquitoes into traps. The effects of baiting BG-Sentinel traps with mice, carbon dioxide (CO2), and attractants derived from litter mouse odours (mouse litter (ML)) and a mouse odour blend (MOB) on the efficiency of trapping Ae. albopictus males and females were tested using a Latin square design. The BG-Sentinel trap baited with CO2 + ML caught a significantly larger number of mosquitoes compared to traps baited with mice only. The BG-Sentinel traps containing only CO2 or CO2 + MOB, however, did not catch significantly more mosquitoes compared to the other traps. The proportions of males caught in the BG-Sentinel traps did not differ significantly between the respective attractants. The results from this study confirm that CO2 bait is efficient to provide a reliable estimation method for Ae. albopictus adult male abundance in the wild, and suggest that mouse litter baits in combination with CO2 could be used to enhance Aedes trapping success in BG-Sentinel traps. [ABSTRACT FROM AUTHOR]

Published
2017
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46. Standard operating procedures for standardized mass rearing of the dengue and chikungunya vectors Aedes aegypti and Aedes albopictus (Diptera: Culicidae) - II - Egg storage and hatching.

Author

Min-Lin Zheng, Dong-Jing Zhang, Damiens, David D., Lees, Rosemary Susan, and Gilles, Jeremie R. L.

Subjects
INSECT rearing, SMALL animal culture, MOSQUITO rearing, AEDES, DENGUE, CHIKUNGUNYA
Abstract

Background: Management of large quantities of eggs will be a crucial aspect of the efficient and sustainable mass production of mosquitoes for programmes with a Sterile Insect Technique component. The efficiency of different hatching media and effectiveness of long term storage methods are presented here. Methods: The effect on hatch rate of storage duration and three hatching media was analysed: deionized water, boiled deionized water and a bacterial broth, using Two-way ANOVA and Post hoc Tukey tests, and the Pearson correlation coefficient was used to find the effect on the proportion of collapsed eggs. Two long term storage methods were also tested: conventional storage (egg paper strips stored in zip lock bags within a sealed plastic box), and water storage (egg papers in a covered plastic cup with deionized water). Regression analyses were used to find the effect of water storage and storage duration on hatch rate. Results: Both species hatched most efficiently in bacterial broth. Few eggs hatched in deionized water, and pre-boiling the water increased the hatch rate of Ae. aegypti, but not Ae. albopictus. A hatch rate greater than 80 % was obtained after 10 weeks of conventional storage in Ae. aegypti and 11 weeks in Ae. albopictus. After this period, hatching decreased dramatically; no eggs hatched after 24 weeks. Storing eggs in water produced an 85 % hatch rate after 5 months in both species. A small but significant proportion of eggs hatched in the water, probably due to combined effects of natural deoxygenation of the water over time and the natural instalment hatching typical of the species. Conclusions: The demonstrated efficiency of the bacterial broth hatching medium for both Ae. albopictus and Ae. aegypti facilitates mass production of these two important vector species in the same facility, with use of a common hatching medium reducing cost and operational complexity. Similarly the increased hatch rate of eggs stored in water would allow greater flexibility of egg management in a large programme over the medium term, particularly if oxygenation of the water by bubbling oxygen through the storage tray could be applied to prevent hatching during storage. [ABSTRACT FROM AUTHOR]

Published
2015
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47. Standard operating procedures for standardized mass rearing of the dengue and chikungunya vectors Aedes aegypti and Aedes albopictus (Diptera: Culicidae) - I - egg quantification.

Author

Min-Lin Zheng, Dong-Jing Zhang, Damiens, David D., Yamada, Hanano, and Gilles, Jeremie R. L.

Subjects
AEDES aegypti, AEDES albopictus, EGGS, INSECT rearing, MOSQUITOES
Abstract

Background Quantification of eggs prior to rearing the immature stages of mosquitoes is an essential step in establishing a standardized mass rearing system. To develop a simple and accurate method of egg quantification for Aedes aegypti and Aedes albopictus, the relationship between egg number and weight, as well as egg number and volume, were studied. Methods Known quantities of eggs (1,000, 3,000, 6,000, 12,000, 15,000, 18,000, 21,000 and 27,000) were counted and subsequently their weight and volume were measured. Best-fit curves and regression equations were used to describe relationships between Aedes egg number and both weight and volume. Results Eighteen thousand Ae. aegypti eggs weighed 159.8 mg and had a volume of 277.4 µl, compared to measurements of 131.5 mg and 230.3 µl for Ae. albopictus. The eggs of Ae. aegypti were thus larger and heavier than those of Ae. albopictus. The use of weight and volume to quantify egg number was validated by counting volumes and weights of eggs expected to correspond to 3,000 and 18,000 eggs of each species; significant correlations were found in all cases except in the case of 3,000 Ae. albopictus eggs measured by volume. Conclusion Methods for egg quantification were validated and shown to be a consistent and practical means to achieve uniform distribution of Aedes larvae between rearing trays, important for optimal mass rearing of the immature stages of Aedes mosquitoes. [ABSTRACT FROM AUTHOR]

Published
2015
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