100 results on '"Dama E"'
Search Results
2. Corrigendum to ‘COVID-19 epidemic strongly affected cancer research in Italy: a survey of the Italian Cancer Society (SIC)’: [ESMO Open Volume 6, Issue 3, June 2021, 100165]
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Bianchi, F., Dama, E., Di Nicolantonio, F., Baldassarre, G., Guerriero, I., Torchiaro, E., Bruno, A., Blandino, G., Allavena, P., Chiarugi, P., Sozzi, G., D’Incalci, M., and Normanno, N.
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- 2021
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3. Failure of locally buckled pipelines
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Dama, E., Karamanos, S.A., and Gresnigt, A.M.
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Fatigue testing machines -- Analysis ,Deformations (Mechanics) -- Analysis ,Numerical analysis -- Usage ,Pipe lines -- Mechanical properties ,Materials -- Fatigue ,Materials -- Analysis ,Engineering and manufacturing industries - Abstract
Mechanical damage in steel pipelines in the form of local buckles due to excessive bending deformation may severely threaten their structural integrity. The present paper describes experimental and numerical research conducted to assess the structural condition of buckled pipes, subjected to both bending and internal pressure. Fatigue failure under repeated loading is mainly investigated, whereas pipe burst due to internal pressure is also examined. Three full-scale buckled pipe specimens are tested under pressure and bending loads to determine their structural capacity. In addition, using nonlinear finite element tools, an extensive parametric study is conducted to determine the critical locations at the buckled area at which maximum strain variation occurs, as well as to investigate the influence of several geometrical and mechanical parameters. Using the maximum strain range from the finite element computations and a simple S-N approach, reasonable predictions are obtained for the number of cycles to failure observed in the tests. The results of the present study demonstrate that, under repeated loading, fatigue failure occurs in the buckled area at the location of maximum strain range. It is also found that the burst pressure may not be affected by the presence of buckles. [DOI: 10.1115/1.2716431]
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- 2007
4. Life expectancy as an indicator of outcome in follow-up of population-based cancer registries: the example of childhood leukemia
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Viscomi, S., Pastore, G., Dama, E., Zuccolo, L., Pearce, N., Merletti, F., and Magnani, C.
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- 2006
- Full Text
- View/download PDF
5. High incidence of acute promyelocytic leukemia in children in northwest Italy, 1980–2003: a report from the Childhood Cancer Registry of Piedmont
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Maule, M M, Dama, E, Mosso, M L, Magnani, C, Pastore, G, and Merletti, F
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- 2008
- Full Text
- View/download PDF
6. Patterns of domestic migrations and access to childhood cancer care centres in Italy: A report from the hospital based registry of the Italian Association of Pediatric Hematology and Oncology (AIEOP)
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Dama, E, Rondelli, R, De Rosa, M, Aricò, M, Carli, M, Bellani, Ff, Magnani, C, Merletti, F, Pastore, G, Pession, A, Madon, E, Dini, G, Carnelli, V, Fedeli, F, Fossati Bellani, F, Masera, G, Locatelli, F, Cornelli, Pe, Notarangelo, L, Nespoli, L, Bagnulo, S, Marradi, P, Musi, L, Rodeghiero, F, Grotto, P, Rossetti, F, Battisti, L, Tamaro, Paolo, Mascarin, M, Nocerino, A, Izzi, G, Paolucci, P, Ambrosioni, G, Picci, P, Borgna Pignatti, C, Vecchi, V, Bernini, G, Morgese, G, Favre, C, Zucchetti, P, Pierani, P, Felici, L, Visani, G, Di Bartolomeo, P, Ballati, G, Castello, Ma, De Rossi, G, Donfrancesco, A, Foà, R, Menichelli, A, Riccardi, R, Di Tullio MT, Fiorillo, A, Poggi, V, Amendola, G, Ladogana, S, Ruggiero, L, Pozzi, S, De Mattia, D, Magro, S, Nobile, F, Sperlì, D, Schilirò, G, Gallisai, D, Biddau, P., Dama, E, Rondelli, R, De Rosa, M, Aricò, M, Carli, M, Bellani, Ff, Magnani, C, Merletti, F, Pastore, G, Pession, A, Madon, E, Dini, G, Carnelli, V, Fedeli, F, Fossati Bellani, F, Masera, G, Locatelli, F, Cornelli, Pe, Notarangelo, L, Nespoli, L, Bagnulo, S, Marradi, P, Musi, L, Rodeghiero, F, Grotto, P, Rossetti, F, Battisti, L, Tamaro, Paolo, Mascarin, M, Nocerino, A, Izzi, G, Paolucci, P, Ambrosioni, G, Picci, P, Borgna Pignatti, C, Vecchi, V, Bernini, G, Morgese, G, Favre, C, Zucchetti, P, Pierani, P, Felici, L, Visani, G, Di Bartolomeo, P, Ballati, G, Castello, Ma, De Rossi, G, Donfrancesco, A, Foà, R, Menichelli, A, Riccardi, R, Di Tullio, Mt, Fiorillo, A, Poggi, V, Amendola, G, Ladogana, S, Ruggiero, L, Pozzi, S, De Mattia, D, Magro, S, Nobile, F, Sperlì, D, Schilirò, G, Gallisai, D, Biddau, P., Dama E, Rondelli R, De Rosa M, Aricò M, Carli M, Bellani FF, Magnani C, Merletti F, Pastore G, Pession A, and Italian Association of Pediatric Hematology and Oncology (AIEOP).
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Oncology ,Male ,Cancer Research ,medicine.medical_specialty ,Adolescent ,Paediatric haematology ,care access ,childhood cancer ,italy ,specialised cancer centres ,Childhood cancer ,Child Health Services ,Regional Medical Programs ,Tertiary care ,Health Services Accessibility ,domestic migration ,Internal medicine ,Neoplasms ,Oncology Service, Hospital ,medicine ,Humans ,Child ,Specialised cancer centres ,Care access ,Italy ,business.industry ,Infant, Newborn ,Infant ,Hospital based ,El Niño ,Child, Preschool ,Residence ,Female ,Health Services Research ,Pediatric hematology ,business ,Delivery of Health Care - Abstract
Tertiary care centres, grouped in the Italian Association of Paediatric Haematology and Oncology (AIEOP) are unevenly distributed across the country. In an attempt to describe their perceived efficacy, we matched the residence and the location of the treatment centre in 18,441 patients aged ⩽15 years treated in the AIEOP network between 1989 and 2005. Overall, centres located in the central and southern regions were less appealing than those located in the North, although this trend decreased over the study period. Patients with solid tumours migrated more frequently than those with leukaemia or lymphoma. Information resulting from better knowledge of the non-random migrations for treatment of children with cancer will be useful to refine planning of the national paediatric haematology-oncology network with social and economic implications.
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- 2008
7. Survival of children with cancer in Italy, 1989-98. A report from the hospital based registry of the Italian Association of Pediatric Haematology and Oncology (AIEOP)
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Pession A, Dama E, Rondelli R, Magnani C, De Rosa M, Locatelli F, Fagioli F, Haupt R, Jankovic M, Terracini B, Merletti F, Pastore G, Italian Association of Paediatric Haematology, Oncology Madon E, Dini G, Carnelli V, Fedeli F, Fossati Bellani F, Masera G, Cornelli PE, Porta F, Dorizzi A, Nespoli A, Carli M, Marradi P, Rodeghiero F, Musi L, Mascarin M, Nocerino A, Izzi G, Paolucci P, Ambrosioni G, Picci P, Borgna Pignatti C, Bernini G, Morgese G, Favre C, Aversa F, Pierani P, Di Marzio A, Foà R, De Rossi G, Donfrancesco A, Castello MA, Casale F, Poggi V, Auricchio S, Antonelli P, Ladogana S, De Mattia D, Magro S, Nobile F, Aricò M, Schilirò G, Gallisai D, Argiolu F., TAMARO, PAOLO, Pession, A, Dama, E, Rondelli, R, Magnani, C, De Rosa, M, Locatelli, F, Fagioli, F, Haupt, R, Jankovic, M, Terracini, B, Merletti, F, Pastore, G, Italian Association of Paediatric, Haematology, Oncology Madon, E, Dini, G, Carnelli, V, Fedeli, F, Fossati Bellani, F, Masera, G, Cornelli, Pe, Porta, F, Dorizzi, A, Nespoli, A, Carli, M, Marradi, P, Rodeghiero, F, Musi, L, Tamaro, Paolo, Mascarin, M, Nocerino, A, Izzi, G, Paolucci, P, Ambrosioni, G, Picci, P, Borgna Pignatti, C, Bernini, G, Morgese, G, Favre, C, Aversa, F, Pierani, P, Di Marzio, A, Foà, R, De Rossi, G, Donfrancesco, A, Castello, Ma, Casale, F, Poggi, V, Auricchio, S, Antonelli, P, Ladogana, S, De Mattia, D, Magro, S, Nobile, F, Aricò, M, Schilirò, G, Gallisai, D, Argiolu, F., Pession A, Dama E, Rondelli R, Magnani C, De Rosa M, Locatelli F, Fagioli F, Haupt R, Jankovic M, Terracini B, Merletti F, Pastore G, and on behalf of the Italian Association of Paediatric Haematology and Oncology
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Oncology ,Male ,Cancer Research ,medicine.medical_specialty ,Pediatrics ,Adolescent ,Lymphoproliferative disorders ,Cancer registration ,survival ,children cancer ,Internal medicine ,Neuroblastoma ,Neoplasms ,medicine ,Humans ,Survivors ,Sex Distribution ,Child ,Survival rate ,Childhood cancer ,Gender ,Italy ,Survival ,Hematology ,business.industry ,Hazard ratio ,Cancer ,Infant ,medicine.disease ,Lymphoma ,El Niño ,Child, Preschool ,Female ,business ,Epidemiologic Methods - Abstract
We describe the survival patterns of 10,791 Italian children (age 0-14) diagnosed with cancer during 1989-1998 and who were included in the hospital-based registry of the Italian Association of Paediatric Haematology and Oncology. Five-year cumulative survival percentages were 76% for lymphoproliferative disorders and 68% for solid tumours. Survival rates in 1994-1998 significantly improved for acute lymphocytic leukaemia (ALL), acute non-lymphocytic leukaemia, Hodgkin's lymphoma and Wilms' tumour. Gender and age were determinants of survival for some specific types of cancer. Girls with ALL and neuroblastoma exhibited a significant advantage (hazard ratio HR 0.72, 0.62-0.83) and disadvantage (HR 0.73, 0.59-0.90) over boys, respectively. Children with a Wilms' tumour diagnosed above age 3 had a worse prognosis than younger children (HR 2.3, 1.4-4.1). The persisting gender-related difference in survival rate for ALL requires understanding as to whether it is attributable to delays in the adoption of more recent therapeutic protocols, while the corresponding findings for Wilms' tumour and neuroblastoma deserve further biological interpretation.
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- 2008
8. Accuracy ofindividual rapid tests for serodiagnosis of gambiense sleeping sickness in west Africa
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Jamonneau, Vincent, Camara, O., Ilboudo, H., Peylhard, M., Koffi, M., Sakande, H., N'Dri, L., Sanou, D., Dama, E., Camara, M., and Lejon, Veerle
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parasitic diseases ,equipment and supplies - Abstract
Background Individual rapid tests for serodiagnosis (RDT) of human African trypanosomiasis (HAT) are particularly suited for passive screening and surveillance. However, so far, no large scale evaluation of RDTs has been performed for diagnosis of Trypanosoma brucei gambiense HAT in West Africa. The objective of this study was to assess the diagnostic accuracy of 2 commercial HAT-RDTs on stored plasma samples from West Africa. Methodology/Principal findings SD Bioline HAT and HAT Sero-K-Set were performed on 722 plasma samples originating from Guinea and Cote d'Ivoire, including 231 parasitologically confirmed HAT patients, 257 healthy controls, and 234 unconfirmed individuals whose blood tested antibody positive in the card agglutination test but negative by parasitological tests. Immune trypanolysis was performed as a reference test for trypanosome specific antibody presence. Sensitivities in HAT patients were respectively 99.6% for SD Bioline HAT, and 99.1% for HAT Sero-K-Set, specificities in healthy controls were respectively 87.9% and 88.3%. Considering combined positivity in both RDTs, increased the specificity significantly (p
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- 2015
9. Marriage and parenthood among childhood cancer survivors: a report from the Italian AIEOP Off-Therapy Registry
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Pivetta, E, Maule, Mm, Pisani, P, Zugna, D, Haupt, R, Jankovic, M, Aricò, M, Casale, F, Clerico, A, Cordero di Montezemolo, L, Kiren, V, Locatelli, F, Palumbo, G, Pession, A, Pillon, M, Santoro, N, Terenziani, M, Valsecchi, Mg, Dama, E, Magnani, C, Merletti, F, Pastore, G, Fagioli, F, Bona, G, Dini, G, Carnelli, V, Biondi, A, Zecca, M, Conter, V, Porta, F, Fedeli, F, Massimino, M, Nespoli, L, Roncarolo, Mg, Carli, M, Cesaro, S, Memo, L, Colleselli, P, Battisti, L, Tamaro, Paolo, Mascarin, M, Nocerino, A, Izzi, G, Paolucci, P, Borgna, Pc, Vecchi, V, Abate, Me, Acquaviva, A, Favre, C, Aversa, F, Pierani, P, Felici, L, Visani, G, Fioritoni, G, Foa, R, Riccardi, R, Frega, G, Poggi, V, Amendola, G, Filosa, A, Ladogana, S, Presta, G, Pozzi, S, De Mattia, D, Consarino, C, Nobile, F, Sperlì, D, D'Angelo, P, Marino, S, Gallisai, D, Targhetta, R., Pivetta, E, Maule, M, Pisani, P, Zugna, D, Haupt, R, Jankovic, M, Aricò, M, Casale, F, Clerico, A, Cordero di Montezemolo, L, Kiren, V, Locatelli, F, Palumbo, G, Pession, A, Pillon, M, Santoro, N, Terenziani, M, Valsecchi, M, Dama, E, Magnani, C, Merletti, F, Pastore, G, Maule, Mm, Valsecchi, Mg, Fagioli, F, Bona, G, Dini, G, Carnelli, V, Biondi, A, Zecca, M, Conter, V, Porta, F, Fedeli, F, Massimino, M, Nespoli, L, Roncarolo, Mg, Carli, M, Cesaro, S, Memo, L, Colleselli, P, Battisti, L, Tamaro, Paolo, Mascarin, M, Nocerino, A, Izzi, G, Paolucci, P, Borgna, Pc, Vecchi, V, Abate, Me, Acquaviva, A, Favre, C, Aversa, F, Pierani, P, Felici, L, Visani, G, Fioritoni, G, Foa, R, Riccardi, R, Frega, G, Poggi, V, Amendola, G, Filosa, A, Ladogana, S, Presta, G, Pozzi, S, De Mattia, D, Consarino, C, Nobile, F, Sperlì, D, D'Angelo, P, Marino, S, Gallisai, D, and Targhetta, R.
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Adult ,Male ,Parents ,Pediatrics ,medicine.medical_specialty ,Total fertility rate ,media_common.quotation_subject ,Population ,Editorials and Perspectives ,Fertility ,off-therapy ,cancer survival ,Cohort Studies ,Quality of life ,Marriage and parenthood ,Medicine ,Humans ,cancer ,cancer survivors ,childhood ,Registries ,Survivors ,Marriage ,education ,Child ,media_common ,education.field_of_study ,business.industry ,Infant, Newborn ,Infant ,Hematology ,Original Articles ,Middle Aged ,childhood cancer ,fertility ,long-term survivors ,marriage ,quality of life ,El Niño ,Italy ,Child, Preschool ,Hematologic Neoplasms ,Cohort ,Marital status ,Female ,business ,Cohort study ,Follow-Up Studies - Abstract
Background The aim of this study was to describe the patterns of marriage and parenthood in a cohort of childhood cancer survivors included in the Off-Therapy Registry maintained by the Italian Association of Pediatric Hematology and Oncology. Design and Methods We analyzed a cohort of 6,044 patients diagnosed with cancer between 1960 and 1998, while aged 0 to 14 years and who were 18 years old or older by December 2003. They were followed up through the regional vital statistics registers until death or the end of follow up (October 30, 2006), whichever occurred first, and their marital status and date of birth of their children were recorded. The cumulative probabilities of being married and having a first child were computed by gender and compared by tumor type within the cohort. Marriage and fertility rates (the latter defined as the number of live births per woman-year) were compared with those of the Italian population of the same age, gender, area of residence and calendar period by means of the observed to expected (O/E) ratios. Results During the follow-up period, 4,633 (77%) subjects had not married. The marriage O/E ratios were 0.56 (95% CI: 0.51-0.61) and 0.70 (95% CI: 0.65-0.76) among men and women, respectively. Overall, 263 men had 367 liveborn children, and 473 women had 697 liveborn children. The female fertility O/E ratio was 0.57 (95% CI: 0.53-0.62) overall, and 1.08 (95% CI: 0.99-1.17) when analyses were restricted to married/cohabiting women Conclusions Childhood cancer survivors are less likely to marry and to have children than the general population, confirming the life-long impact of their previous disease on their social behavior and choices. The inclusion of counseling in the strategies of management and long-term surveillance of childhood cancer patients could be beneficial to survivors as they approach adulthood. ©2011 Ferrata Storti Foundation.
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- 2011
10. Migrazioni in bambini registrati dai centri AIEOP con MOD.1.01 nel periodo 1989-2005
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Dama E., Rondelli R., Pastore G., PESSION, ANDREA, Dama E., Rondelli R., Pastore G., and Pession A.
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- 2007
11. Evaluating Access to Pediatric Cancer Care Centers of Children and Adolescents With Rare Tumors in Italy: The TREP Project
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Pastore, G, DE SALVO GL, Bisogno, Gianni, Dama, E, Inserra, A, Cecchetto, Giovanni, and Ferrari, A.
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- 2009
12. Italian cancer figures--report 2008. 1. Childhood cancer
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Magnani, C, Baussano, I, Buzzoni, C, Capocaccia, R, Crocetti, E, Dalmasso, P, Dama, E, Maule, M, Pannelli, F, Pascucci, C, Pastore, G, Puppo, A, Russo, A, Tognazzo, S, Paci, E, AIRTUM WORKING GROUP, Vercelli, Marina, Marani, E, Casella, C, Celesia, M. V., Cogno, R, Grondona, A. M., Giachero, G, and Orengo, Ma
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Childhood cancers - survival - time trends - Published
- 2008
13. Frequenza e prognosi dei tumori infantili in Piemonte. Il Registro dei Tumori Infantili del Piemonte 1967-2004
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Alessi, D, Pastore, G, Bagnati, R, Baussano, I, Dalmasso, Paola, Dama, E, Gregori, D, Maule, MILENA MARIA, Mosso, Ml, Terracini, Benedetto, Zanetti, R, Zuccolo, L, Merletti, Franco, and Magnani, C.
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- 2006
14. Late deaths among five-year survivors of childhood cancer. A population-based study in Piedmont Region, Italy
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Dama, E., Pastore, G., Mosso, M. L., Daniela Ferrante, Maule, M. M., Magnani, C., and Merletti, F.
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Time Factors ,Treatment Outcome ,Italy ,Neoplasms ,Humans ,Registries ,Survivors ,Child ,Survival Analysis ,Follow-Up Studies - Abstract
The aim of this study was to analyze late mortality among 5-year survivors of childhood cancer, in Piedmont (Italy), in terms of risk factors and causes of death.From 1967 to 1999, the Childhood Cancer Registry of Piedmont recorded 3164 incident cases. Patients identified only by a death certificate (n = 59), lost to follow-up (n = 32), alive with a period of observation shorter than 5 years at the end of follow-up (n = 65) and records corresponding to a second malignant tumor during childhood (n = 9) were excluded from the analyses.Within 5 years after diagnosis, 1301 children died, and among the 1698 5- year survivors, 144 children subsequently died. Among 5-year survivors, cumulative mortality percentages increased from 5.1% (95% CI 4.0-6.2) at 10 years after diagnosis to 16.0% (12.2-19.8) at 35 years. Period of diagnosis (p = 0.006), age at diagnosis (p = 0.002), and tumor type (p = 0.003) were associated with late mortality. Most deaths were related to cancer recurrence (62.2%) and treatment-related sequelae (22.4%), including second malignant neoplasms, cardiac diseases and other late effects. Compared to the general population, children included in this study had a 9-fold increased risk of overall mortality, and experienced an absolute excess of 4.4 deaths per 1000 person-years.Among 5-year survivors, patients treated more recently (after 1979) had a statistically significant lower risk of late death than those treated earlier. However, long-term survivors still experienced higher mortality rates than those in the general population, and recurrence or progression of the primary tumor was the first cause of death.
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- 2006
15. Influence of chemiotherapy on the expansion of the abdominal aortic aneurysm in patient with neoplastic disease
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Conforti, M., Barile, G., Dama, E., Varetto, Gianfranco, Casella, F., Santovito, Davide, and Rispoli, Pietro
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- 2005
16. 23O SERUM MICRORNA AS A DIAGNOSTIC TEST FOR EARLY LUNG CANCER
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Veronesi, G., primary, Bianchi, F., additional, Rampinelli, C., additional, Maisonneuve, P., additional, Dama, E., additional, Bonizzi, G., additional, Bellomi, M., additional, Spaggiari, L., additional, Travaini, L., additional, and Di Fiore, P., additional
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- 2013
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17. High incidence of acute promyelocytic leukemia in children in northwest Italy, 1980–2003: a report from the Childhood Cancer Registry of Piedmont
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Maule, M M, primary, Dama, E, additional, Mosso, M L, additional, Magnani, C, additional, Pastore, G, additional, and Merletti, F, additional
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- 2007
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18. Expected number of childhood cancers in Italy from 2001 to 2015
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Baussano, I., primary, Maule, M. M., additional, Dama, E., additional, Dalmasso, P., additional, Mosso, M. L., additional, Galzerano, M., additional, Merletti, F., additional, Magnani, C., additional, and Pastore, G., additional
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- 2007
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19. Failure of Locally Buckled Pipelines
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Dama, E., primary, Karamanos, S. A., additional, and Gresnigt, A. M., additional
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- 2006
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20. Patterns of domestic migrations and access to childhood cancer care centres in Italy: a report from the hospital based registry of the Italian Association of Pediatric Hematology and Oncology (AIEOP)
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Dama E, Rondelli R, De Rosa M, Aricò M, Carli M, Bellani FF, Magnani C, Merletti F, Pastore G, and Pession A
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Tertiary care centres, grouped in the Italian Association of Paediatric Haematology and Oncology (AIEOP) are unevenly distributed across the country. In an attempt to describe their perceived efficacy, we matched the residence and the location of the treatment centre in 18,441 patients aged less-than-or-equals, slant15 years treated in the AIEOP network between 1989 and 2005.Overall, centres located in the central and southern regions were less appealing than those located in the North, although this trend decreased over the study period. Patients with solid tumours migrated more frequently than those with leukaemia or lymphoma.Information resulting from better knowledge of the non-random migrations for treatment of children with cancer will be useful to refine planning of the national paediatric haematology-oncology network with social and economic implications. [ABSTRACT FROM AUTHOR]
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- 2008
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21. Immune trypanolysis test as a promising bioassay to monitor the elimination of gambiense human African trypanosomiasis
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Dama Emilie, Camara Oumou, Kaba Dramane, Koffi Mathurin, Camara Mamadou, Compaoré Charlie, Ilboudo Hamidou, Courtin Fabrice, Kaboré Jacques, N’Gouan Emmanuel Kouassi, Büscher Philippe, Lejon Veerle, Bucheton Bruno, and Jamonneau Vincent
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human african trypanosomiasis ,trypanosoma brucei gambiense ,elimination ,diagnosis ,transmission ,immune trypanolysis ,Infectious and parasitic diseases ,RC109-216 - Abstract
The World Health Organization (WHO) has set the goal of gambiense-Human African trypanosomiasis (HAT) elimination as a public health problem for 2020 and interruption of transmission in humans for 2030. In this context, it is crucial to monitor progress towards these targets using accurate tools to assess the level of transmission in a given area. The aim of this study was to investigate the relevance of the immune trypanolysis test (TL) as a population-based bioassay to evaluate Trypanosoma brucei gambiense transmission in various epidemiological contexts. Significant correlations were observed between HAT endemicity levels and the percentage of TL-positive individuals in the population. TL therefore appears to be a suitable population-based biomarker of the intensity of transmission. In addition to being used as a tool to assess the HAT status at an individual level, assessing the proportion of TL positive individuals in the population appears as a promising and easy alternative to monitor the elimination of gambiense HAT in a given area.
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- 2019
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22. Diagnostic tools for human African trypanosomiasis elimination and clinical trials
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Lejon, V., Mumba, D., Ngay, I., Camara, M., Camara, O., Kaba, D., Kone, M., Lumbala, C., Makabuza, J., Ilboudo, H., Dama, E., Fevre, E., Vincent Jamonneau, Bucheton, B., and Buscher, P.
23. Epidemiological aspects of craniopharyngioma
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Haupt, R., Magnani, C., Pavenello, M., Caruso, S., Dama, E., and Maria Luisa Garrè
24. Two cases of rare neck tumors: A vagal paraganglioma and a hypoglossal nerve paraganglioma | Due casi di rari tumori del collo: Un paraganglioma del nervo vago e un paraganglioma del nervo ipoglosso
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Maselli, M., Conforti, M., Rispoli, P., Apostolou, D., Ortensio, M., Scovazzi, P., Gianfranco Varetto, Dama, E., and Raso, A. M.
25. Influence of chemotherapy on the expansion of abdominal aortic aneurysms in patients with neoplastic disease | Influenza della chemioterapia sull'evoluzione degli aneurismi dell'aorta addominale in paziente neoplastici
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Conforti, M., Barile, G., Dama, E., Gianfranco Varetto, Casella, F., Santovito, D., and Rispoli, P.
26. Adolescents with cancer in Australia: comparative assessment of the number of cases observed in AIEOP centers and cases expected
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Pastore, G., Arico, M., Masera, G., Merletti, F., Maurizio Mascarin, Ferrari, S., Locatelli, F., Pascucci, C., Rondelli, R., Pession, A., Dama, E., and Ferrari, A.
27. Thoracic ultrasound combined with low-dose computed tomography may represent useful screening strategy in highly exposed population in the industrial city of Taranto (Italy)
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Quarato, Carla Maria Irene, Dama, Elisa, Maggi, Michele, Feragalli, Beatrice, Borelli, Cristina, Del Colle, Anna, Taurchini, Marco, Maiello, Evaristo, De Cosmo, Salvatore, Lacedonia, Donato, Barbaro, Maria Pia Foschino, Carpagnano, Giovanna Elisiana, Scioscia, Giulia, Graziano, Paolo, Termine, Rosalinda, Frongillo, Elisabettamaria, Santamaria, Sonia, Venuti, Mariapia, Grimaldi, Maria Arcangela, Notarangelo, Stefano, Saponara, Annarita, Copetti, Massimiliano, Colangelo, Tommaso, Cuttano, Roberto, Macrodimitris, Dimitrios, Mazzarelli, Francesco, Talia, Michela, Mirijello, Antonio, Pazienza, Luca, Perna, Rita, Simeone, Anna, Vergara, Doriana, Varriale, Antonio, Carella, Massimo, Bianchi, Fabrizio, Sperandeo, Marco, Quarato, C, Dama, E, Maggi, M, Feragalli, B, Borelli, C, Del Colle, A, Taurchini, M, Maiello, E, De Cosmo, S, Lacedonia, D, Barbaro, M, Carpagnano, G, Scioscia, G, Graziano, P, Termine, R, Frongillo, E, Santamaria, S, Venuti, M, Grimaldi, M, Notarangelo, S, Saponara, A, Copetti, M, Colangelo, T, Cuttano, R, Macrodimitris, D, Mazzarelli, F, Talia, M, Mirijello, A, Pazienza, L, Perna, R, Simeone, A, Vergara, D, Varriale, A, Carella, M, Bianchi, F, and Sperandeo, M
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ransthoracic ultrasound, high-resolution computed tomography, lung cancer, interstitial lung abnormalities, screening protocol, environmental exposure ,lung cancer ,interstitial lung abnormalities ,high-resolution computed tomography ,screening protocol ,ransthoracic ultrasound ,environmental exposure ,transthoracic ultrasound ,General Medicine ,interstitial lung abnormalitie - Abstract
ObjectivesWe validated a screening protocol in which thoracic ultrasound (TUS) acts as a first-line complementary imaging technique in selecting patients which may deserve a second-line low-dose high resolution computed tomography (HRCT) scan among a population of asymptomatic high-risk subjects for interstitial lung abnormalities (ILA) and lung cancer. Due to heavy environmental pollution burden, the district Tamburi of Taranto has been chosen as “case study” for this purpose.MethodsFrom July 2018 to October 2020, 677 patients aged between 45 and 65 year and who had been living in the Tamburi district of Taranto for at least 10 years were included in the study. After demographic, clinical and risk factor exposition data were collected, each participant underwent a complete TUS examination. These subjects were then asked to know if they agreed to perform a second-level examination by low-dose HRCT scan.ResultsOn a total of 167 subjects (24.7%) who agreed to undergo a second-level HRCT, 85 patients (50.9%) actually showed pleuro-pulmonary abnormalities. Interstitial abnormalities were detected in a total of 36 patients on HRCT scan. In particular, 34 participants presented subpleural ILAs, that were classified in the fibrotic subtype in 7 cases. The remaining 2 patients showed non-subpleural interstitial abnormalities. Subpleural nodules were observed in 46 patients. TUS showed an overall diagnostic accuracy of 88.6% in detecting pleuro-pulmonary abnormalities in comparison with HRCT scan, with a sensitivity of 95.3%, a specificity of 81.7%, a positive predictive value of 84.4% and a negative predictive value of 94.4%. The matched evaluation of specific pulmonary abnormalities on HRTC scan (i.e., interstitial abnormalities or pulmonary nodules) with determinate sonographic findings revealed a reduction in both TUS sensibility and specificity. Focusing TUS evaluation on the assessment of interstitial abnormalities, a thickened pleural line showed a sensitivity of 63.9% and a specificity of 69.5%, hypoechoic striae showed a sensitivity of 38.9% and a specificity of 90.1% and subpleural nodules showed a sensitivity of 58.3% and a specificity of 77.1%. Regarding to the assessment of subpleural nodules, TUS showed a sensitivity of 60.9% and a specificity of 81.0%. However, the combined employment of TUS examination and HRCT scans allowed to identify 34 patients with early subpleural ILA and to detect three suspicious pulmonary nodules (of which two were intraparenchymal and one was a large subpleural mass), which revealed to be lung cancers on further investigations.ConclusionA first-line TUS examination might aid the identification of subjects highly exposed to environmental pollution, who could benefit of a second-line low-dose HRCT scan to find early interstitial lung diseases as well as lung cancer.Protocol registration codePLEURO-SCREENING-V1.0_15 Feb, 17.
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- 2023
28. miRNome profiling of lung cancer metastases revealed a key role for miRNA-PD-L1 axis in the modulation of chemotherapy response
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Roberto Cuttano, Tommaso Colangelo, Juliana Guarize, Elisa Dama, Maria Pia Cocomazzi, Francesco Mazzarelli, Valentina Melocchi, Orazio Palumbo, Elena Marino, Elena Belloni, Francesca Montani, Manuela Vecchi, Massimo Barberis, Paolo Graziano, Andrea Pasquier, Julian Sanz-Ortega, Luis M. Montuenga, Cristiano Carbonelli, Lorenzo Spaggiari, Fabrizio Bianchi, Cuttano, R, Colangelo, T, Guarize, J, Dama, E, Cocomazzi, M, Mazzarelli, F, Melocchi, V, Palumbo, O, Marino, E, Belloni, E, Montani, F, Vecchi, M, Barberis, M, Graziano, P, Pasquier, A, Sanz-Ortega, J, Montuenga, L, Carbonelli, C, Spaggiari, L, and Bianchi, F
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PD-L1 ,Cancer Research ,microRNA ,Oncology ,Chemotherapy ,Gene expression ,Lung cancer ,NSCLC ,miR-455-5p ,Hematology ,Molecular Biology - Abstract
Locally advanced non-small cell lung cancer (NSCLC) is frequent at diagnosis and requires multimodal treatment approaches. Neoadjuvant chemotherapy (NACT) followed by surgery is the treatment of choice for operable locally advanced NSCLC (Stage IIIA). However, the majority of patients are NACT-resistant and show persistent lymph nodal metastases (LNmets) and an adverse outcome. Therefore, the identification of mechanisms and biomarkers of NACT resistance is paramount for ameliorating the prognosis of patients with Stage IIIA NSCLC. Here, we investigated the miRNome and transcriptome of chemo-naïve LNmets collected from patients with Stage IIIA NSCLC (N = 64). We found that a microRNA signature accurately predicts NACT response. Mechanistically, we discovered a miR-455-5p/PD-L1 regulatory axis which drives chemotherapy resistance, hallmarks metastases with active IFN-γ response pathway (an inducer of PD-L1 expression), and impacts T cells viability and relative abundances in tumor microenvironment (TME). Our data provide new biomarkers to predict NACT response and add molecular insights relevant for improving the management of patients with locally advanced NSCLC.
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- 2022
29. Marriage and parenthood among subjects cured of childhood cancer: a report from the Italian AIEOP Off-Therapy Registry
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Pivetta, EMANUELE EMILIO, Maule, MILENA MARIA, Pisani, Paola, Zugna, Daniela, Haupt, R, Jankovic, M, Arico', M, Casale, F, Clerico, A, CORDERO DI MONTEZEMOLO, Luca, Kiren, V, Locatelli, F, Palumbo, G, Pession, A, Pillon, M, Santoro, N, Terenziani, M, Valsecchi, Mg, Dama, Elisa, Magnani, C, Merletti, Franco, Pastore, G., Pivetta E., Maule MM., Pisani P., Zugna D., Haupt R., Jankovic M., Arico' M., Casale F., Clerico A., Cordero di Montezemolo L., Kiren V., Locatelli F., Palumbo G., Pession A., Pillon M., Santoro N., Terenziani M., Valsecchi MG., Dama E., Magnani C., Merletti F., Pastore G., Pivetta, E, Maule, Mm, Pisani, P, Zugna, D, Haupt, R, Jankovic, M, Aricò, M, Casale, Fiorina, Clerico, A, CORDERO DI MONTEZEMOLO, L, Kiren, V, Locatelli, F, Palumbo, G, Pession, A, Pillon, M, Santoro, N, Terenziani, M, Valsecchi, Mg, Dama, E, Magnani, C, Merletti, F, and Pastore, G.
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Quality of life ,Fertility ,childhood cancer ,Marriage ,Long-term survivors ,childhood cancer, marriage, fertility, long-term survivors, quality of life - Abstract
The aim of this study was to describe the patterns of marriage and parenthood in a cohort of childhood cancer survivors included in the Off-Therapy Registry maintained by the Italian Association of Pediatric Hematology and Oncology. Design and Methods We analyzed a cohort of 6,044 patients diagnosed with cancer between 1960 and 1998, while aged 0 to 14 years and who were 18 years old or older by December 2003. They were followed up through the regional vital statistics registers until death or the end of follow up (October 30, 2006), whichever occurred first, and their marital status and date of birth of their children were recorded. The cumulative probabilities of being married and having a first child were computed by gender and compared by tumor type within the cohort. Marriage and fertility rates (the latter defined as the number of live births per woman-year) were compared with those of the Italian population of the same age, gender, area of residence and calendar period by means of the observed to expected (O/E) ratios. Results During the follow-up period, 4,633 (77%) subjects had not married. The marriage O/E ratios were 0.56 (95% CI: 0.51-0.61) and 0.70 (95% CI: 0.65-0.76) among men and women, respectively. Overall, 263 men had 367 liveborn children, and 473 women had 697 liveborn children. The female fertility O/E ratio was 0.57 (95% CI: 0.53-0.62) overall, and 1.08 (95% CI: 0.99-1.17) when analyses were restricted to married/cohabiting women Conclusions Childhood cancer survivors are less likely to marry and to have children than the general population, confirming the life-long impact of their previous disease on their social behavior and choices. The inclusion of counseling in the strategies of management and long-term surveillance of childhood cancer patients could be beneficial to survivors as they approach adulthood.
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- 2011
30. Loss of circadian gene Timeless induces EMT and tumor progression in colorectal cancer via Zeb1-dependent mechanism
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Tommaso Colangelo, Annalucia Carbone, Francesco Mazzarelli, Roberto Cuttano, Elisa Dama, Teresa Nittoli, Jacopo Albanesi, Giovannina Barisciano, Nicola Forte, Orazio Palumbo, Paolo Graziano, Alessandra di Masi, Vittorio Colantuoni, Lina Sabatino, Fabrizio Bianchi, Gianluigi Mazzoccoli, Colangelo, Tommaso, Carbone, Annalucia, Mazzarelli, Francesco, Cuttano, Roberto, Dama, Elisa, Nittoli, Teresa, Albanesi, Jacopo, Barisciano, Giovannina, Forte, Nicola, Palumbo, Orazio, Graziano, Paolo, di Masi, Alessandra, Colantuoni, Vittorio, Sabatino, Lina, Bianchi, Fabrizio, Mazzoccoli, Gianluigi, Colangelo, T, Carbone, A, Mazzarelli, F, Cuttano, R, Dama, E, Nittoli, T, Albanesi, J, Barisciano, G, Forte, N, Palumbo, O, Graziano, P, di Masi, A, Colantuoni, V, Sabatino, L, Bianchi, F, and Mazzoccoli, G
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Metastasis prognostic markers ,TIMELESS ,Epithelial-Mesenchymal Transition ,Intracellular Signaling Peptides and Proteins ,Zinc Finger E-box-Binding Homeobox 1 ,colorectal cancer ,Cell Cycle Proteins ,Cell Biology ,Gene Expression Regulation, Neoplastic ,Cell Line, Tumor ,ZEB1 ,Humans ,Colorectal Neoplasms ,Molecular Biology - Abstract
The circadian gene Timeless (TIM) provides a molecular bridge between circadian and cell cycle/DNA replication regulatory systems and has been recently involved in human cancer development and progression. However, its functional role in colorectal cancer (CRC), the third leading cause of cancer-related deaths worldwide, has not been fully clarified yet. Here, the analysis of two independent CRC patient cohorts (total 1159 samples) reveals that loss of TIM expression is an unfavorable prognostic factor significantly correlated with advanced tumor stage, metastatic spreading, and microsatellite stability status. Genome-wide expression profiling, in vitro and in vivo experiments, revealed that TIM knockdown induces the activation of the epithelial-to-mesenchymal transition (EMT) program. Accordingly, the analysis of a large set of human samples showed that TIM expression inversely correlated with a previously established gene signature of canonical EMT markers (EMT score), and its ectopic silencing promotes migration, invasion, and acquisition of stem-like phenotype in CRC cells. Mechanistically, we found that loss of TIM expression unleashes ZEB1 expression that in turn drives the EMT program and enhances the aggressive behavior of CRC cells. Besides, the deranged TIM-ZEB1 axis sets off the accumulation of DNA damage and delays DNA damage recovery. Furthermore, we show that the aggressive and genetically unstable ‘CMS4 colorectal cancer molecular subtype’ is characterized by a lower expression of TIM and that patients with the combination of low-TIM/high-ZEB1 expression have a poorer outcome. In conclusion, our results as a whole suggest the engagement of an unedited TIM-ZEB1 axis in key pathological processes driving malignant phenotype acquisition in colorectal carcinogenesis. Thus, TIM-ZEB1 expression profiling could provide a robust prognostic biomarker in CRC patients, supporting targeted therapeutic strategies with better treatment selection and patients’ outcomes.
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- 2022
31. Integrated molecular profiling of patient-derived ovarian cancer models identifies clinically relevant signatures and tumor vulnerabilities
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Michela Lupia, Valentina Melocchi, Francesca Bizzaro, Pietro Lo Riso, Elisa Dama, Micol Baronio, Alberto Ranghiero, Massimo Barberis, Loris Bernard, Giovanni Bertalot, Raffaella Giavazzi, Giuseppe Testa, Fabrizio Bianchi, Ugo Cavallaro, Lupia, M, Melocchi, V, Bizzaro, F, Lo Riso, P, Dama, E, Baronio, M, Ranghiero, A, Barberis, M, Bernard, L, Bertalot, G, Giavazzi, R, Testa, G, Bianchi, F, and Cavallaro, U
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Ovarian Neoplasms ,Cancer Research ,cancer stem cell ,Ascites ,Carcinoma, Ovarian Epithelial ,Prognosis ,Cystadenocarcinoma, Serous ,genomic ,Phosphatidylinositol 3-Kinases ,ascite ,ovarian cancer ,Oncology ,Humans ,Female ,xenograft ,prognosi - Abstract
High-grade serous ovarian carcinoma (HGSOC) is a highly aggressive and intractable neoplasm, mainly because of its rapid dissemination into the abdominal cavity, a process that is favored by tumor-associated peritoneal ascites. The precise molecular alterations involved in HGSOC onset and progression remain largely unknown due to the high biological and genetic heterogeneity of this tumor. We established a set of different tumor samples (termed the As11-set) derived from a single HGSOC patient, consisting of peritoneal ascites, primary tumor cells, ovarian cancer stem cells (OCSC) and serially propagated tumor xenografts. The As11-set was subjected to an integrated RNA-seq and DNA-seq analysis which unveiled molecular alterations that marked the different types of samples. Our profiling strategy yielded a panel of signatures relevant in HGSOC and in OCSC biology. When such signatures were used to interrogate the TCGA dataset from HGSOC patients, they exhibited prognostic and predictive power. The molecular alterations also identified potential vulnerabilities associated with OCSC, which were then tested functionally in stemness-related assays. As a proof of concept, we defined PI3K signaling as a novel druggable target in OCSC.
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- 2021
32. Biomarkers and Lung Cancer Early Detection: State of the Art
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Fabrizio Bianchi, Marco Cremonesi, Tommaso Colangelo, Emanuela Fina, Giulia Veronesi, Marinos Kallikourdis, Elisa Dama, Dama, E., Colangelo, T., Fina, E., Cremonesi, M., Kallikourdis, M., Veronesi, G., and Bianchi, F.
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Oncology ,Prioritization ,Cancer Research ,medicine.medical_specialty ,Early detection ,Review ,Disease ,Circulating tumor cell ,Lung cancer early detection ,Internal medicine ,medicine ,Liquid biopsy ,Lung cancer ,RC254-282 ,liquid biopsy ,business.industry ,biomarkers ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Early diagnosis ,medicine.disease ,lung cancer ,Therapy response ,business ,Biomarkers ,early diagnosis - Abstract
Simple Summary Lung cancer is the leading cause of cancer death worldwide. Detecting lung malignancies promptly is essential for any anticancer treatment to reduce mortality and morbidity, especially in high-risk individuals. The use of liquid biopsy to detect circulating biomarkers such as RNA, microRNA, DNA, proteins, autoantibodies in the blood, as well as circulating tumor cells (CTCs), can substantially change the way we manage lung cancer patients by improving disease stratification using intrinsic molecular characteristics, identification of therapeutic targets and monitoring molecular residual disease. Here, we made an update on recent developments in liquid biopsy-based biomarkers for lung cancer early diagnosis, and we propose guidelines for an accurate study design, execution, and data interpretation for biomarker development. Abstract Lung cancer burden is increasing, with 2 million deaths/year worldwide. Current limitations in early detection impede lung cancer diagnosis when the disease is still localized and thus more curable by surgery or multimodality treatment. Liquid biopsy is emerging as an important tool for lung cancer early detection and for monitoring therapy response. Here, we reviewed recent advances in liquid biopsy for early diagnosis of lung cancer. We summarized DNA- or RNA-based biomarkers, proteins, autoantibodies circulating in the blood, as well as circulating tumor cells (CTCs), and compared the most promising studies in terms of biomarkers prediction performance. While we observed an overall good performance for the proposed biomarkers, we noticed some critical aspects which may complicate the successful translation of these biomarkers into the clinical setting. We, therefore, proposed a roadmap for successful development of lung cancer biomarkers during the discovery, prioritization, and clinical validation phase. The integration of innovative minimally invasive biomarkers in screening programs is highly demanded to augment lung cancer early detection.
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- 2021
33. Aggressive early-stage lung adenocarcinoma is characterized by epithelial cell plasticity with acquirement of stem-like traits and immune evasion phenotype
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Tommaso Colangelo, Giulia Veronesi, Giuseppe Pelosi, Francesco Mazzarelli, Leonarda Di Candia, Gian Maria Ferretti, Paolo Graziano, Marco Taurchini, Elisa Dama, Valentina Melocchi, Roberto Cuttano, Enrico Lugli, Fabrizio Bianchi, Melocchi, V., Dama, E., Mazzarelli, F., Cuttano, R., Colangelo, T., Di Candia, L., Lugli, E., Veronesi, G., Pelosi, G., Ferretti, G. M., Taurchini, M., Graziano, P., and Bianchi, F.
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0301 basic medicine ,Cancer Research ,Phenotypic screening ,Cell Plasticity ,Adenocarcinoma of Lung ,Respiratory Mucosa ,Disease ,Biology ,Transcriptome ,03 medical and health sciences ,0302 clinical medicine ,Lung Cancer ,Molecular Subtype ,Prognosis ,Tumor Microenvironment ,Genetics ,medicine ,Humans ,Cell Lineage ,Progenitor cell ,Lung cancer ,Molecular Biology ,Immune Evasion ,Neoplasm Staging ,Gene Expression Profiling ,Computational Biology ,medicine.disease ,Phenotype ,030104 developmental biology ,030220 oncology & carcinogenesis ,Mutation ,Cancer cell ,Neoplastic Stem Cells ,Cancer research ,Adenocarcinoma ,Disease Susceptibility ,Biomarkers - Abstract
Lung adenocarcinoma (LUAD) is the main non-small-cell lung cancer diagnosed in ~40-50% of all lung cancer cases. Despite the improvements in early detection and personalized medicine, even a sizable fraction of patients with early-stage LUAD would experience disease relapses and adverse prognosis. Previous reports indicated the existence of LUAD molecular subtypes characterized by specific gene expression and mutational profiles, and correlating with prognosis. However, the biological and molecular features of such subtypes have not been further explored. Consequently, the mechanisms driving the emergence of aggressive LUAD remained unclear. Here, we adopted a multi-tiered approach ranging from molecular to functional characterization of LUAD and used it on multiple cohorts of patients (for a total of 1227 patients) and LUAD cell lines. We investigated the tumor transcriptome and the mutational and immune gene expression profiles, and we used LUAD cell lines for cancer cell phenotypic screening. We found that loss of lung cell lineage and gain of stem cell-like characteristics, along with mutator and immune evasion phenotypes, explain the aggressive behavior of a specific subset of lung adenocarcinoma that we called C1-LUAD, including early-stage disease. This subset can be identified using a 10-gene prognostic signature. Poor prognosis patients appear to have this specific molecular lung adenocarcinoma subtype which is characterized by peculiar molecular and biological features. Our data support the hypothesis that transformed lung stem/progenitor cells and/or reprogrammed epithelial cells with CSC characteristics are hallmarks of this aggressive disease. Such discoveries suggest alternative, more aggressive, therapeutic strategies for early-stage C1-LUAD.
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- 2021
34. PROGNOSTIC METHOD FOR AGGRESSIVE LUNG ADENOCARCINOMAS
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BIANCHI, Fabrizio, DAMA, Elisa, MELOCCHI, Valentina, Bianchi, F, Dama, E, and Melocchi, V
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miRNA biomarker ,lung adenocarcinoma ,prognosi - Published
- 2020
35. Most high-grade neuroendocrine tumours of the lung are likely to secondarily develop from pre-existing carcinoids: innovative findings skipping the current pathogenesis paradigm
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Sara Pilotto, Fiorella Calabrese, Sergio Harari, Fabrizio Bianchi, Giuseppe Pelosi, Andrea Mafficini, Gabriella Fontanini, Marco Volante, Aldo Scarpa, Michele Simbolo, Luca Mastracci, Angelica Sonzogni, Elisa Dama, Adriana Albini, Emilio Bria, Mauro Papotti, Pelosi, G, Bianchi, F, Dama, E, Simbolo, M, Mafficini, A, Sonzogni, A, Pilotto, S, Harari, S, Papotti, M, Volante, M, Fontanini, G, Mastracci, L, Albini, A, Bria, E, Calabrese, F, and Scarpa, A
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0301 basic medicine ,Male ,Lung Neoplasms ,Cluster analysis ,Lung ,Neuroendocrine ,Transition ,Tumours ,Aged ,Carcinoid Tumor ,Cluster Analysis ,Disease Progression ,Female ,Humans ,Middle Aged ,Neuroendocrine Tumors ,2734 ,Molecular Biology ,Cell Biology ,Sequencing data ,Neuroendocrine tumors ,Pathology and Forensic Medicine ,Pathogenesis ,03 medical and health sciences ,0302 clinical medicine ,Medicine ,Large-cell neuroendocrine carcinoma ,Cluster analysi ,business.industry ,General Medicine ,medicine.disease ,respiratory tract diseases ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Cancer research ,Typical carcinoid ,Small Cell Lung Carcinoma ,business ,Atypical carcinoid - Abstract
Among lung neuroendocrine tumours (Lung-NETs), typical carcinoid (TC) and atypical carcinoid (AC) are considered separate entities as opposed to large cell neuroendocrine carcinoma (LCNEC) and small cell lung carcinoma (SCLC). By means of two-way clustering analysis of previously reported next-generation sequencing data on 148 surgically resected Lung-NETs, six histology-independent clusters (C1 → C6) accounting for 68% of tumours were identified. Low-grade Lung-NETs were likely to evolve into high-grade tumours following two smoke-related paths. Tumour composition of the first path (C5 → C1 → C6) was coherent with the hypothesis of an evolution of TC to LCNEC, even with a conversion of SCLC-featuring tumours to LCNEC. The second path (C4 → C2-C3) had a tumour composition supporting the evolution of AC to SCLC-featuring tumours. The relevant Ki-67 labelling index varied accordingly, with median values being 5%, 9% and 50% in the cluster sequence C5 → C1 → C6, 12% in cluster C4 and 50-60% in cluster C2-C3. This proof-of-concept study suggests an innovative view on the progression of pre-existing TC or AC to high-grade NE carcinomas in most Lung-NET instances.
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- 2018
36. Optimization and Standardization of Circulating MicroRNA Detection for Clinical Application: The miR-Test Case
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Massimo Bellomi, Lorenzo Spaggiari, Cristiano Rampinelli, Rose Mary Carletti, Fabrizio Bianchi, Francesco Nicassio, Maria Teresa Sandri, Pier Paolo Di Fiore, Matteo Jacopo Marzi, Francesca Montani, Fabio Dezi, Patrick Maisonneuve, Giulia Veronesi, Elisa Dama, Giuseppina Bonizzi, Marzi, Mj, Montani, F, Carletti, Rm, Dezi, F, Dama, E, Bonizzi, G, Sandri, Mt, Rampinelli, C, Bellomi, M, Maisonneuve, P, Spaggiari, L, Veronesi, G, Bianchi, F, Di Fiore, Pp, and Nicassio, F
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0301 basic medicine ,Validation study ,Standardization ,Operating procedures ,Biochemistry (medical) ,Clinical Biochemistry ,Transferability ,Computational biology ,Biology ,Bioinformatics ,Serum samples ,Real-Time Polymerase Chain Reaction ,03 medical and health sciences ,Circulating MicroRNA ,MicroRNAs ,030104 developmental biology ,Lung cancer early detection ,Humans ,RNA extraction - Abstract
BACKGROUND The identification of circulating microRNAs (miRNAs) in the blood has been recently exploited for the development of minimally invasive tests for the early detection of cancer. Nevertheless, the clinical transferability of such tests is uncertain due to still-insufficient standardization and optimization of methods to detect circulating miRNAs in the clinical setting. METHODS We performed a series of tests to optimize the quantification of serum miRNAs that compose the miR-Test, a signature for lung cancer early detection, and systematically analyzed variables that could affect the performance of the test. We took advantage of a large-scale (>1000 samples) validation study of the miR-Test that we recently published, to evaluate, in clinical samples, the effects of analytical and preanalytical variables on the quantification of circulating miRNAs and the clinical output of the signature (risk score). RESULTS We developed a streamlined and standardized pipeline for the processing of clinical serum samples that allows the isolation and analysis of circulating miRNAs by quantitative reverse-transcription PCR, with a throughput compatible with screening trials. The major source of analytical variation came from RNA isolation from serum, which could be corrected by use of external (spike-in) or endogenous miRNAs as a reference for normalization. We also introduced standard operating procedures and QC steps to check for unspecific fluctuations that arise from the lack of standardized criteria in the collection or handling of the samples (preanalytical factors). CONCLUSIONS We propose our methodology as a reference for the development of clinical-grade blood tests on the basis of miRNA detection.
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- 2015
37. miR-Test: A Blood Test for Lung Cancer Early Detection
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Pier Paolo Di Fiore, Matteo Jacopo Marzi, Patrick Maisonneuve, Francesco Nicassio, Francesca Montani, Giuseppina Bonizzi, Massimo Bellomi, Raffaella Bertolotti, Fabio Dezi, Rose Mary Carletti, Fabrizio Bianchi, Giulia Veronesi, Cristiano Rampinelli, Elisa Dama, Lorenzo Spaggiari, Montani, F, Marzi, Mj, Dezi, F, Dama, E, Carletti, Rm, Bonizzi, G, Bertolotti, R, Bellomi, M, Rampinelli, C, Maisonneuve, P, Spaggiari, L, Veronesi, G, Nicassio, F, Di Fiore, Pp, and Bianchi, F
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Adult ,Male ,Oncology ,Cancer Research ,medicine.medical_specialty ,Lung Neoplasms ,Population ,Sensitivity and Specificity ,Predictive Value of Tests ,Internal medicine ,Biomarkers, Tumor ,medicine ,Humans ,Blood test ,Lung cancer ,education ,Early Detection of Cancer ,Aged ,education.field_of_study ,Cancer Death Rate ,medicine.diagnostic_test ,business.industry ,Smoking ,Cancer ,Middle Aged ,medicine.disease ,Confidence interval ,Surgery ,MicroRNAs ,Area Under Curve ,Population Surveillance ,Predictive value of tests ,Female ,Tomography, X-Ray Computed ,business ,Lung cancer screening - Abstract
Lung cancer is the leading cause of cancer death worldwide. Low-dose computed tomography screening (LDCT) was recently shown to anticipate the time of diagnosis, thus reducing lung cancer mortality. However, concerns persist about the feasibility and costs of large-scale LDCT programs. Such concerns may be addressed by clearly defining the target "high-risk" population that needs to be screened by LDCT. We recently identified a serum microRNA signature (the miR-Test) that could identify the optimal target population. Here, we performed a large-scale validation study of the miR-Test in high-risk individuals (n = 1115) enrolled in the Continuous Observation of Smoking Subjects (COSMOS) lung cancer screening program. The overall accuracy, sensitivity, and specificity of the miR-Test are 74.9% (95% confidence interval [CI] = 72.2% to 77.6%), 77.8% (95% CI = 64.2% to 91.4%), and 74.8% (95% CI = 72.1% to 77.5%), respectively; the area under the curve is 0.85 (95% CI = 0.78 to 0.92). These results argue that the miR-Test might represent a useful tool for lung cancer screening in high-risk individuals.
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- 2015
38. Implementation and performance evaluation of an integrated specimen referral system in Burkina Faso using the national courier services (2020-2022).
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Dama E, Porgho S, Ake YC, Yameogo I, Gampini S, Adjami AA, Nikiema A, Kamate M, Tarbangdo F, Sawadogo R, Sawadogo C, Ouedraogo HS, Zerbo H, Rahalison L, Medah I, Dahourou AG, Greco-Kone R, and Ake FH
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- Burkina Faso, Humans, Specimen Handling, Referral and Consultation, SARS-CoV-2, COVID-19
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Introduction: In 2017, the Ministry of Health and Public Hygiene (MoH) of Burkina Faso designed and piloted a specimen transport system using the national courier services (La Poste BF) in 4 districts. Based on satisfactory performance indicators, the MoH set a vision aimed at scaling up this system to strengthen disease detection and surveillance of epidemic prone diseases across the country. This work describes the implementation process, performances, and lessons learned., Methodology: This work describes the implementation process, performances, and lessons learned. Under the leadership of the Directorate of Population Health Protection within the MoH, a stepwise approach was used to bring together multiple partners across sectors to develop the first needed documents including a guide, an implementation plan, Standard Operating Procedures, and data collection tools. Then, the execution phase included equipment purchase, trainings, and consensus on a financing mechanism. Key indicators were defined to allow performance monitoring., Result: The integrated biological specimen referral system (SITEB) was officially launched in January 2020 to transport human biological specimens of priority diseases including COVID-19 from district level to reference laboratories nationwide. As of December 31, 2022, La Poste BF transported 168,856 packages containing 206,314 specimens from all 13 regions. 99.66% of packages were delivered in <24 h as required, and 99.68% of specimens were in good condition at reception. COVID-19 specimens represented respectively 18% and 63% of samples transported in 2020 and 2021., Discussion: The political will combined with the experience gained during the pilot phase and the commitment and support from all stakeholders laid to the foundation of the effective implementation of this system. Collaboration between two government entities (MoH and Minister of Transport, Urban Mobility, and Road Safety) to benefit public health has led to reasonable pricing for sustainability. Although all documents integrate the "One Health" approach, the system ensures the transport of only human samples for now. Despite security constraints, Burkina Faso has successfully set up a system using the national postal service to ensure the routine transport of specimens for all diseases under laboratory surveillance including laboratory tests for HIV and TB from the district level to reference laboratories nationwide. This system has also proved to be useful and efficient in managing public health emergency., Competing Interests: Y-CA, MK, FT, and FA were employed by DAVYCAS International. AN was employed by Integrated Quality Laboratory Services (IQLS). The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The reviewer AA declared a shared affiliation with the authors ED, RS, LR, AD, and RG-K to the handling editor at the time of review., (Copyright © 2024 Dama, Porgho, Ake, Yameogo, Gampini, Adjami, Nikiema, Kamate, Tarbangdo, Sawadogo, Sawadogo, Ouedraogo, Zerbo, Rahalison, Medah, Dahourou, Greco-Kone and Ake.)
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- 2024
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39. MicroRNAs and the Mediterranean diet: a nutri-omics perspective for lung cancer.
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Cuttano R, Mazzarelli F, Afanga KM, Bianchi F, and Dama E
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- Humans, Nutrigenomics, Lung Neoplasms prevention & control, Lung Neoplasms genetics, Diet, Mediterranean, MicroRNAs genetics, MicroRNAs metabolism
- Abstract
Lung cancer is the deadliest cancer type worldwide with ~ 1.8 million deaths per-year. Smoking accounts for ~ 85% of all cases, with a described joint effect with unhealthy diet in lung cancer risk increase. Public health policies to prevent carcinogens exposure, promote smoking cessation and advocacy for healthy nutrition, are therefore highly recommended. Here we have examined the benefits of the Mediterranean Diet (MedDiet) in protecting against some non-communicable diseases including lung cancer, highlighting the epidemiological and biomolecular aspects of MedDiet anti-inflammatory effect and its interaction with smoking habits closely linked to risk of lung cancer. Considering the high incidence and mortality rates of lung cancer, we discussed also about the global impact that a Planeterranean extension of the benefits of MedDiet could have on controlling lung cancer risk. We also debated the impact of personalized nutrition on lung cancer prevention, considering individual heterogeneity in response to diet plans as well as recent advancements on nutri-omics in lung cancer research, with a specific focus on the role of microRNAs (miRNAs) as a promising nutritional molecular hub for lung cancer prevention. We strongly believe that a deep understanding of the molecular link between food components and genetic/epigenetics factors can expand effective intervention strategies., (© 2024. The Author(s).)
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- 2024
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40. An in-silico analysis reveals further evidence of an aggressive subset of lung carcinoids sharing molecular features of high-grade neuroendocrine neoplasms.
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Pelosi G, Melocchi V, Dama E, Hofman P, De Luca M, Albini A, Gemelli M, Ricotta R, Papotti M, La Rosa S, Uccella S, Harari S, Sonzogni A, Asiedu MK, Wigle DA, and Bianchi F
- Subjects
- Humans, DNA Copy Number Variations genetics, Lung pathology, DNA Helicases genetics, Nuclear Proteins genetics, Transcription Factors genetics, Receptor-Like Protein Tyrosine Phosphatases, Class 5 genetics, Lung Neoplasms genetics, Lung Neoplasms pathology, Neuroendocrine Tumors genetics, Neuroendocrine Tumors pathology, Carcinoma, Neuroendocrine genetics, Carcinoid Tumor genetics, Carcinoid Tumor pathology, Carcinoma, Large Cell genetics, Carcinoma, Large Cell pathology
- Abstract
Little is known as to whether there may be any pathogenetic link between pulmonary carcinoids and neuroendocrine carcinomas (NECs). A gene signature we previously found to cluster pulmonary carcinoids, large cell neuroendocrine carcinoma (LCNEC) and small cell lung carcinoma (SCLC), and which encompassed MEN1, MYC, MYCL1, RICTOR, RB1, SDHA, SRC and TP53 mutations or copy number variations (CNVs), was used to reclassify an independent cohort of 54 neuroendocrine neoplasms (NENs) [31 typical carcinoids (TC), 11 atypical carcinoids (AC) and 12 SCLC], by means of transcriptome and mutation data. Unsupervised clustering analysis identified two histology-independent clusters, namely CL1 and CL2, where 17/42 (40.5%) carcinoids and all the SCLC samples fell into the latter. CL2 carcinoids affected survival adversely, were enriched in T to G transversions or T > C/C > T transitions in the context of specific mutational signatures, presented with at least 1.5-fold change (FC) increase of gene mutations including TSC2, SMARCA2, SMARCA4, ERBB4 and PTPRZ1, differed for gene expression and showed epigenetic changes in charge of MYC and MTORC1 pathways, cellular senescence, inflammation, high-plasticity cell state and immune system exhaustion. Similar results were also found in two other independent validation sets comprising 101 lung NENs (24 carcinoids, 21 SCLC and 56 LCNEC) and 30 carcinoids, respectively. We herein confirmed an unexpected sharing of molecular traits along the spectrum of lung NENs, with a subset of genomically distinct aggressive carcinoids sharing molecular features of high-grade neuroendocrine neoplasms., Competing Interests: Declaration of competing interest The author has no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties., (Copyright © 2023. Published by Elsevier Inc.)
- Published
- 2024
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41. Sentinel laboratory compliance with best practices in Burkina Faso's antimicrobial resistance surveillance network.
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Yenyetou D, Zongo E, Dama E, Muhigwa M, Sanou I, Sawadogo C, Ouangraoua S, Sangare I, Nikiema A, Dahourou AG, and Ouedraogo AS
- Abstract
Background: Standardising procedures is the best way to harmonise and strengthen the quality of laboratory-based antimicrobial resistance surveillance. Since 2018, Burkina Faso has developed and disseminated the national manual of procedures for performing antibiotic susceptibility tests in sentinel laboratories within its national antimicrobial resistance surveillance network., Objective: Our study aimed to assess these sentinel laboratories' compliance with good practices for antibiotics susceptibility tests., Methods: Four teams evaluated the antimicrobial resistance sentinel sites laboratories throughout Burkina Faso from 19 to 28 September 2022. Eighteen out of 19 sentinel laboratories were evaluated. A four-member technical committee designed and validated the evaluation tool composed of three Microsoft Excel sheets. The evaluation emphasised quality controls for culture media, antibiotic discs and compliance with antimicrobial susceptibility testing procedures by the laboratories. Excel software was used for data recording and graphs and table design. The free R software version 4.2.0 was used for descriptive statistics. An overall score below 80% was considered noncompliance., Results: Most (83.33%) of the sentinel laboratories conducted at least one quality control activity for culture media, and 66.67% conducted at least one quality control activity for antibiotic discs. Over three-quarters (76.47%) of the laboratories were more than 80% compliant with the modified Kirby Bauer antimicrobial susceptibility testing method., Conclusion: The evaluation revealed the noncompliance of sentinel laboratories with the national procedure manual, particularly in the quality control component., What This Study Adds: This study has provided baseline data on the sentinel laboratories' compliance with the national antimicrobial susceptibility testing procedures manual, particularly in areas performing quality control checks or meeting quality indicators for culture media and antibiotic discs., Competing Interests: The authors declare that they have no financial or personal relationships that may have inappropriately influenced them in writing this article., (© 2024. The Authors.)
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- 2024
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42. On-site evaluation as external quality assessment of microbiology laboratories involved in sentinel laboratory-based antimicrobial resistance surveillance network in Burkina Faso.
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Zongo E, Dama E, Yenyetou D, Muhigwa M, Nikiema A, Dahourou GA, and Ouedraogo AS
- Subjects
- Humans, Burkina Faso, Cross-Sectional Studies, Laboratories, Anti-Bacterial Agents pharmacology, Drug Resistance, Bacterial
- Abstract
Background: The laboratory-based surveillance of antimicrobial resistance (AMR) is a priority component of the multisectoral national action plan to combat AMR in Burkina Faso. This study aimed to assess the QMS of microbiology laboratories involved in the Sentinel laboratory-based antimicrobial resistance surveillance network in Burkina Faso., Methods: A cross-sectional study was conducted from September 1st to November 30
th, 2022. The external quality assessment (EQA) method used was on-site evaluation using a checklist that was developed and validated by a technical committee of experts. Teams of two, including an antimicrobial susceptibility test (AST) specialist and a QMS specialist, were trained on this checklist to conduct the assessment. Satisfactory performance was defined as any on-site evaluation score 80% and above with the aim of developing action plans to address gaps., Results: All 18 laboratories were evaluated. The overall average performance score of the participating laboratories was 40%. The highest overall performance score was 58%, and the lowest overall performance score was 26%. The average overall scores were not significantly different between private and public laboratories (p value = 0.78). The only section of the checklist with the satisfactory performance concerned the "Analytical step of AST", with 76.5% (13/17) of the sentinel laboratories having a score ≥ 80%., Conclusion: The performance of the QMS of the sentinel laboratories in Burkina Faso for AMR surveillance was unsatisfactory, and a corrective action plan was proposed to support these laboratories in improving their QMS over the next 3 years., (© 2024. The Author(s).)- Published
- 2024
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43. Thoracic ultrasound combined with low-dose computed tomography may represent useful screening strategy in highly exposed population in the industrial city of Taranto (Italy).
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Quarato CMI, Dama E, Maggi M, Feragalli B, Borelli C, Del Colle A, Taurchini M, Maiello E, De Cosmo S, Lacedonia D, Barbaro MPF, Carpagnano GE, Scioscia G, Graziano P, Termine R, Frongillo E, Santamaria S, Venuti M, Grimaldi MA, Notarangelo S, Saponara A, Copetti M, Colangelo T, Cuttano R, Macrodimitris D, Mazzarelli F, Talia M, Mirijello A, Pazienza L, Perna R, Simeone A, Vergara D, Varriale A, Carella M, Bianchi F, and Sperandeo M
- Abstract
Objectives: We validated a screening protocol in which thoracic ultrasound (TUS) acts as a first-line complementary imaging technique in selecting patients which may deserve a second-line low-dose high resolution computed tomography (HRCT) scan among a population of asymptomatic high-risk subjects for interstitial lung abnormalities (ILA) and lung cancer. Due to heavy environmental pollution burden, the district Tamburi of Taranto has been chosen as "case study" for this purpose., Methods: From July 2018 to October 2020, 677 patients aged between 45 and 65 year and who had been living in the Tamburi district of Taranto for at least 10 years were included in the study. After demographic, clinical and risk factor exposition data were collected, each participant underwent a complete TUS examination. These subjects were then asked to know if they agreed to perform a second-level examination by low-dose HRCT scan., Results: On a total of 167 subjects (24.7%) who agreed to undergo a second-level HRCT, 85 patients (50.9%) actually showed pleuro-pulmonary abnormalities. Interstitial abnormalities were detected in a total of 36 patients on HRCT scan. In particular, 34 participants presented subpleural ILAs, that were classified in the fibrotic subtype in 7 cases. The remaining 2 patients showed non-subpleural interstitial abnormalities. Subpleural nodules were observed in 46 patients. TUS showed an overall diagnostic accuracy of 88.6% in detecting pleuro-pulmonary abnormalities in comparison with HRCT scan, with a sensitivity of 95.3%, a specificity of 81.7%, a positive predictive value of 84.4% and a negative predictive value of 94.4%. The matched evaluation of specific pulmonary abnormalities on HRTC scan (i.e., interstitial abnormalities or pulmonary nodules) with determinate sonographic findings revealed a reduction in both TUS sensibility and specificity. Focusing TUS evaluation on the assessment of interstitial abnormalities, a thickened pleural line showed a sensitivity of 63.9% and a specificity of 69.5%, hypoechoic striae showed a sensitivity of 38.9% and a specificity of 90.1% and subpleural nodules showed a sensitivity of 58.3% and a specificity of 77.1%. Regarding to the assessment of subpleural nodules, TUS showed a sensitivity of 60.9% and a specificity of 81.0%. However, the combined employment of TUS examination and HRCT scans allowed to identify 34 patients with early subpleural ILA and to detect three suspicious pulmonary nodules (of which two were intraparenchymal and one was a large subpleural mass), which revealed to be lung cancers on further investigations., Conclusion: A first-line TUS examination might aid the identification of subjects highly exposed to environmental pollution, who could benefit of a second-line low-dose HRCT scan to find early interstitial lung diseases as well as lung cancer., Protocol Registration Code: PLEURO-SCREENING-V1.0_15 Feb, 17., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Quarato, Dama, Maggi, Feragalli, Borelli, Del Colle, Taurchini, Maiello, De Cosmo, Lacedonia, Barbaro, Carpagnano, Scioscia, Graziano, Termine, Frongillo, Santamaria, Venuti, Grimaldi, Notarangelo, Saponara, Copetti, Colangelo, Cuttano, Macrodimitris, Mazzarelli, Talia, Mirijello, Pazienza, Perna, Simeone, Vergara, Varriale, Carella, Bianchi and Sperandeo.)
- Published
- 2023
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44. miRNome profiling of lung cancer metastases revealed a key role for miRNA-PD-L1 axis in the modulation of chemotherapy response.
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Cuttano R, Colangelo T, Guarize J, Dama E, Cocomazzi MP, Mazzarelli F, Melocchi V, Palumbo O, Marino E, Belloni E, Montani F, Vecchi M, Barberis M, Graziano P, Pasquier A, Sanz-Ortega J, Montuenga LM, Carbonelli C, Spaggiari L, and Bianchi F
- Subjects
- Humans, B7-H1 Antigen genetics, B7-H1 Antigen metabolism, Biomarkers, Tumor Microenvironment, Lung Neoplasms drug therapy, Lung Neoplasms genetics, Lung Neoplasms metabolism, Carcinoma, Non-Small-Cell Lung drug therapy, Carcinoma, Non-Small-Cell Lung genetics, Carcinoma, Non-Small-Cell Lung metabolism, MicroRNAs genetics, MicroRNAs therapeutic use
- Abstract
Locally advanced non-small cell lung cancer (NSCLC) is frequent at diagnosis and requires multimodal treatment approaches. Neoadjuvant chemotherapy (NACT) followed by surgery is the treatment of choice for operable locally advanced NSCLC (Stage IIIA). However, the majority of patients are NACT-resistant and show persistent lymph nodal metastases (LNmets) and an adverse outcome. Therefore, the identification of mechanisms and biomarkers of NACT resistance is paramount for ameliorating the prognosis of patients with Stage IIIA NSCLC. Here, we investigated the miRNome and transcriptome of chemo-naïve LNmets collected from patients with Stage IIIA NSCLC (N = 64). We found that a microRNA signature accurately predicts NACT response. Mechanistically, we discovered a miR-455-5p/PD-L1 regulatory axis which drives chemotherapy resistance, hallmarks metastases with active IFN-γ response pathway (an inducer of PD-L1 expression), and impacts T cells viability and relative abundances in tumor microenvironment (TME). Our data provide new biomarkers to predict NACT response and add molecular insights relevant for improving the management of patients with locally advanced NSCLC., (© 2022. The Author(s).)
- Published
- 2022
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45. Strengthening Biorisk Management Capacities in Burkina Faso: Contribution of the Global Health Security Agenda.
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Dama E, Orelle A, Nikiema A, Mandingar PD, Naby A, Bationo GB, Kéita MS, Pierson A, Sawadogo C, and Koné RG
- Subjects
- Humans, Laboratories, World Health Organization, Risk Management, Global Health, Containment of Biohazards methods
- Abstract
The Global Health Security Agenda and the International Health Regulations (2005) recommend that countries strengthen the capacity of their national laboratory systems to comply with the International Health Regulations. To efficiently and effectively direct these efforts, the US Centers for Disease Control and Prevention-in collaboration with the Ministry of Health Directorate of Laboratories, the African Society for Laboratory Medicine, and Integrated Quality Laboratory Service-assessed Burkina Faso's national laboratory system using the World Health Organization Laboratory Assessment Tool . Based on gaps observed in biorisk management, the Biosafety and Biosecurity Laboratory Assessment Tool (BSS LAT) was developed to assess 10 public laboratories handling dangerous pathogens. This tool uses a specific scoring matrix with quantitative output. Composite assessment scores for the 9 primary modules (capacity areas) were reported, with the highest scores reported in cleaning, disinfection, sterilization, waste management (42%), and good laboratory practices (40%), and the lowest scores in biosecurity/biosafety (33%), documents/regulations (18%), emergency management (16%), and risk management (5%). To address challenges identified in the assessments, a set of activities was planned with a focus on biorisk management. Results from an evaluation conducted 1 year later, using the BSS LAT, showed an increase in the average score of all indicators from 25% to 45% and an increase in the biorisk management module score from 5% to 35%. This evaluation process was a decisive step toward strengthening the capacity of the laboratory system in Burkina Faso. Global Health Security Agenda investments and activities have made a lasting impact on improving biosafety and biosecurity in public health laboratories. To ensure sustainability, a strong laboratory quality management program based on a mentorship system is greatly needed.
- Published
- 2022
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46. Evaluation of antibody responses to tsetse fly saliva in domestic animals in the sleeping sickness endemic foci of Bonon and Sinfra, Côte d'Ivoire.
- Author
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Somda MB, N'Djetchi MK, Kaboré J, Ilboudo H, Dama E, Boma S, Courtin F, Poinsignon A, Bengaly Z, Remoué F, Belem AMG, Bucheton B, Jamonneau V, and Koffi M
- Subjects
- Animals, Animals, Domestic, Antibody Formation, Cattle, Cote d'Ivoire epidemiology, Humans, Sheep, Swine, Cattle Diseases parasitology, Sheep Diseases, Swine Diseases parasitology, Trypanosoma, Trypanosomiasis, African epidemiology, Trypanosomiasis, African parasitology, Trypanosomiasis, African veterinary, Tsetse Flies parasitology
- Abstract
After intensive control efforts, human African trypanosomiasis (HAT) was declared eliminated in Côte d'Ivoire as a public health problem in December 2020 and the current objective is to achieve the interruption of the transmission (zero cases). Reaching this objective could be hindered by the existence of an animal reservoir of Trypanosoma (T.) brucei (b.) gambiense. In the framework of a study led in 2013 to assess the role of domestic animals in the epidemiology of HAT in the two last active foci from Côte d'Ivoire (Bonon and Sinfra), plasmas were sampled from four species of domestic animals for parasitological (microscopic examination by the buffy coat technique (BCT)), serological (immune trypanolysis (TL)) and molecular (specific PCR: TBR for T. brucei s.l., TCF for T. congolense forest type, TVW for T. vivax and PCR for T. b. gambiense) testing. In order to improve the understanding of the involvement/role of these animals in the transmission of T. b. gambiense, we have quantified in this study the IgG response to whole saliva extracts of Glossina palpalis gambiensis in order to perform an association analysis between anti-saliva responses and the positivity of diagnostic tests. Cattle and pigs had significantly higher rates of anti-tsetse saliva responses compared to goats and sheep (p < 0.01). In addition, the anti-tsetse saliva responses were strongly associated with the parasitology (BCT+), serology (TL+) and PCR (TBR+ and TCF+) results (p < 0.001). These associations indicate a high level of contacts between the positive/infected animals and tsetse flies. Our findings suggest that protecting cattle and pigs against tsetse bites could have a significant impact in reducing transmission of both animal and human trypanosome species, and advocates for a "One health" approach to better control African trypanosomosis in Côte d'Ivoire., (Copyright © 2022 Elsevier B.V. All rights reserved.)
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- 2022
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47. Loss of circadian gene Timeless induces EMT and tumor progression in colorectal cancer via Zeb1-dependent mechanism.
- Author
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Colangelo T, Carbone A, Mazzarelli F, Cuttano R, Dama E, Nittoli T, Albanesi J, Barisciano G, Forte N, Palumbo O, Graziano P, di Masi A, Colantuoni V, Sabatino L, Bianchi F, and Mazzoccoli G
- Subjects
- Cell Line, Tumor, Gene Expression Regulation, Neoplastic, Humans, Cell Cycle Proteins genetics, Colorectal Neoplasms genetics, Colorectal Neoplasms pathology, Epithelial-Mesenchymal Transition, Intracellular Signaling Peptides and Proteins genetics, Zinc Finger E-box-Binding Homeobox 1 genetics
- Abstract
The circadian gene Timeless (TIM) provides a molecular bridge between circadian and cell cycle/DNA replication regulatory systems and has been recently involved in human cancer development and progression. However, its functional role in colorectal cancer (CRC), the third leading cause of cancer-related deaths worldwide, has not been fully clarified yet. Here, the analysis of two independent CRC patient cohorts (total 1159 samples) reveals that loss of TIM expression is an unfavorable prognostic factor significantly correlated with advanced tumor stage, metastatic spreading, and microsatellite stability status. Genome-wide expression profiling, in vitro and in vivo experiments, revealed that TIM knockdown induces the activation of the epithelial-to-mesenchymal transition (EMT) program. Accordingly, the analysis of a large set of human samples showed that TIM expression inversely correlated with a previously established gene signature of canonical EMT markers (EMT score), and its ectopic silencing promotes migration, invasion, and acquisition of stem-like phenotype in CRC cells. Mechanistically, we found that loss of TIM expression unleashes ZEB1 expression that in turn drives the EMT program and enhances the aggressive behavior of CRC cells. Besides, the deranged TIM-ZEB1 axis sets off the accumulation of DNA damage and delays DNA damage recovery. Furthermore, we show that the aggressive and genetically unstable 'CMS4 colorectal cancer molecular subtype' is characterized by a lower expression of TIM and that patients with the combination of low-TIM/high-ZEB1 expression have a poorer outcome. In conclusion, our results as a whole suggest the engagement of an unedited TIM-ZEB1 axis in key pathological processes driving malignant phenotype acquisition in colorectal carcinogenesis. Thus, TIM-ZEB1 expression profiling could provide a robust prognostic biomarker in CRC patients, supporting targeted therapeutic strategies with better treatment selection and patients' outcomes., (© 2022. The Author(s), under exclusive licence to ADMC Associazione Differenziamento e Morte Cellulare.)
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- 2022
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48. Integrated molecular profiling of patient-derived ovarian cancer models identifies clinically relevant signatures and tumor vulnerabilities.
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Lupia M, Melocchi V, Bizzaro F, Lo Riso P, Dama E, Baronio M, Ranghiero A, Barberis M, Bernard L, Bertalot G, Giavazzi R, Testa G, Bianchi F, and Cavallaro U
- Subjects
- Ascites genetics, Carcinoma, Ovarian Epithelial pathology, Female, Humans, Phosphatidylinositol 3-Kinases, Prognosis, Cystadenocarcinoma, Serous, Ovarian Neoplasms genetics, Ovarian Neoplasms pathology
- Abstract
High-grade serous ovarian carcinoma (HGSOC) is a highly aggressive and intractable neoplasm, mainly because of its rapid dissemination into the abdominal cavity, a process that is favored by tumor-associated peritoneal ascites. The precise molecular alterations involved in HGSOC onset and progression remain largely unknown due to the high biological and genetic heterogeneity of this tumor. We established a set of different tumor samples (termed the As11-set) derived from a single HGSOC patient, consisting of peritoneal ascites, primary tumor cells, ovarian cancer stem cells (OCSC) and serially propagated tumor xenografts. The As11-set was subjected to an integrated RNA-seq and DNA-seq analysis which unveiled molecular alterations that marked the different types of samples. Our profiling strategy yielded a panel of signatures relevant in HGSOC and in OCSC biology. When such signatures were used to interrogate the TCGA dataset from HGSOC patients, they exhibited prognostic and predictive power. The molecular alterations also identified potential vulnerabilities associated with OCSC, which were then tested functionally in stemness-related assays. As a proof of concept, we defined PI3K signaling as a novel druggable target in OCSC., (© 2022 The Authors. International Journal of Cancer published by John Wiley & Sons Ltd on behalf of UICC.)
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- 2022
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49. Evaluation of the Re-emergence Risk of Human African Trypanosomiasis in the Southwestern Burkina Faso, A Gold-Bearing Mutation Area.
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Somda MB, Kaboré J, Karambiri SM, Dama E, Dabiré, Compaoré CFA, Salou EW, Ilboudo H, Houaga I, Courtin F, Belem AMG, Jamonneau V, and Bengaly Z
- Subjects
- Animals, Burkina Faso epidemiology, Gold, Humans, Mutation, Swine, Trypanosoma, Trypanosomiasis, African epidemiology
- Abstract
Purpose: The boom in Burkina Faso's artisanal gold mining since 2007 has attracted populations from Côte d'Ivoire and Guinea, which are the West African countries most affected by human African trypanosomiasis (HAT) and therefore increases its risk of re-emergence. Our aim was to update the HAT data in Burkina Faso in the risk of the re-emergence context with the advent of artisanal gold mining., Methods: The study was carried out in the southwestern Burkina Faso where entomological surveys were conducted using biconical traps in March 2017. Follow by an active medical survey in April 2017, which was targeted the gold panners in 7 villages closer to artisanal gold sites, using CATT, mini-anion exchange centrifugation technique, trypanolysis test (TL) and ELISA test to measure human/tsetse contacts. The buffy coat technique and the TL were also applied in pigs to check their reservoir role of human trypanosomes., Results: Our results have shown no case of HAT among 958 individuals tested and all the 50 pigs were also negative, but the level of antibodies against tsetse saliva evidenced by ELISA revealed low human/tsetse contact. Moreover, gold panners practise agriculture and breeding in an infected tsetse area, which are increased the risk., Conclusion: Our results illustrate that the risk of re-emergence is low. The passive surveillance system implemented in 2015 in southwestern Burkina Faso is needed to increase the sentinel sites to better cover this area by taking into account the gold mining. Finally, awareness-raising activities are needed among populations about HAT., (© 2022. The Author(s) under exclusive licence to Witold Stefański Institute of Parasitology, Polish Academy of Sciences.)
- Published
- 2022
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50. A Subset of Large Cell Neuroendocrine Carcinomas in the Gastroenteropancreatic Tract May Evolve from Pre-existing Well-Differentiated Neuroendocrine Tumors.
- Author
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Pelosi G, Bianchi F, Dama E, Metovic J, Barella M, Sonzogni A, Albini A, Papotti M, Gong Y, and Vijayvergia N
- Subjects
- Adult, Aged, Aged, 80 and over, Cluster Analysis, DNA Mutational Analysis, Female, Humans, Male, Middle Aged, Carcinoma, Neuroendocrine genetics, Carcinoma, Neuroendocrine pathology, Intestinal Neoplasms genetics, Intestinal Neoplasms pathology, Neuroendocrine Tumors genetics, Neuroendocrine Tumors pathology, Pancreatic Neoplasms genetics, Pancreatic Neoplasms pathology, Stomach Neoplasms genetics, Stomach Neoplasms pathology
- Abstract
In the gastro-entero-pancreatic (GEP) tract, neuroendocrine neoplasms (NENs) include well differentiated neuroendocrine tumors (NETs) and high-grade NE carcinomas (NECs), which are thought to make up separate and mutually exclusive tumor entities. Little is known, however, as to whether there may be any pathogenetic link between them. Clustering analysis of a 10-gene panel generated from a previously reported next-generation sequencing analysis on 48 GEP-NENs with clinical annotations was used in the study. Unsupervised cluster analysis showed three histology-independent clusters, namely, C1, C2, and C3, which accounted for 44% of patients but the entire array of mutations. All but two NECs fell into the clusters, yet with different prevalence rates (p < 0.0001). A model was devised according to which NETs were likely to evolve into NECs upon progression of C3 into C1 and C2, despite different morphology. The median Ki-67 labeling index was 5% in C3 showing better prognosis and 50% in C1 and C2 experiencing worse prognosis, with an impressive intra-tumor heterogeneity of diversely proliferating tumor areas. This study suggests that a subset of large cell NECs in the gastroenteropancreatic tract may evolve from pre-existing well-differentiated NETs., (© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC part of Springer Nature.)
- Published
- 2021
- Full Text
- View/download PDF
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