33 results on '"Dajić, Ana"'
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2. A decision support tool for municipal waste landfills' management.
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Mihajlović, Marina, Dajić, Ana, and Papić, Miloš
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- 2024
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- View/download PDF
3. Microfluidic technology for macro systems: Removal of textile dyes from wastewater in a microreactor
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Svetozarević Arsović Milica, Šekuljica Nataša, Dajić Ana, Mihajlović Marina, Marković Maja, Knežević-Jugović Zorica, and Mijin Dušan
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Environmental sciences ,GE1-350 - Abstract
Wastewater from textile industry contains considerable amount of dissolved dye that can trigger environmental issues if is not treated properly. Numerous methods have been developed to degrade recalcitrant pollutants safely and utterly. Among them, enzymatic treatment of wastewater is gaining attention due to the enzyme’s specificity, easier manipulation, and generation of less toxic by-products. Still, the cost of enzymatic systems is the main limitation keeping the biocatalysts at lab-scale. Alternative solution for reducing the cost of enzymatic reaction systems is the use of microfluidic systems, which contribute to better mixing, process intensification and cleaner production. In this study, implementation of horseradish peroxidase for removal of the textile dye Acid Violet 109 is performed in a microfluidic reactor. The microreactor consists of three plunger pump units, two mixers and PTFE tube. The process parameters: residence time, dye, hydrogen peroxide, enzyme activity, the reactors’ diameter and length were optimized. Under the optimal process conditions: 30 mg/L dye concentration, 0.8 U/mL horseradish peroxidase activity, 0.1 mM hydrogen peroxide, 0.25 mm reactor’s diameter, 97,3 % removal was achieved at residence time of 6 min. The results from this study show that enzymatic microfluidic reactors are a convenient technology for dye removal.
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- 2023
- Full Text
- View/download PDF
4. The effect of climate change on the potential for landfill gas generation at the Vinča landfill site
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Dajić Ana, Mihajlović Marina, and Arsović Milica Svetozarević
- Subjects
Environmental sciences ,GE1-350 - Abstract
Climate change projections indicate that Republic of Serbia and the Western Balkans will face a high probability of continuing temperature increases, climatic extremes are projected to become more common, including a significant increase in the number of extreme heat events. Heavier precipitation events are expected in the winter months, whilst summers are projected to become even drier. This paper aims to analyse effect of precipitation changes on landfill gas generation on the Vinča landfill, the biggest landfill in Serbia. Quantities of generated landfill gas and methane have been estimated by using LandGem model. Site specific methane generation rate has been calculated according to GMI methodology for Central Eastern Europe. Sensitivity analyses is performed to determine influence of a precipitation regimes on the methane generation rate on the site. Any increase in precipitation (in range 10-30%) may increase landfill gas production for 4.3%. The paper shows the difference in gas emissions, with the same composition of waste, and different precipitation regimes. Changes in precipitation regimes due to climate change can affect the dynamics of landfill gas emissions. This information is significant for the possible re-circulation of leachate from the landfill which could result in an increased production of landfill gas.
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- 2023
- Full Text
- View/download PDF
5. UKLANJANJE BOJE ACID VIOLET 109 IZ OTPADNE VODE TEKSTILNE INDUSTRIJE UNAPEĐENIM PROCESOM OKSIDACIJE.
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Dajić, Ana and Mihajlović, Marina
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DYES & dyeing ,WASTEWATER treatment ,OXIDATION ,HABER-Weiss reaction ,MAINTENANCE costs - Abstract
Copyright of Proceedings of the International Congress on Process Engineering - Processing is the property of Union of Mechanical & Electrotechnical Engineers & Technicians of Serbia (SMEITS) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2024
- Full Text
- View/download PDF
6. Microfluidic technology for macro systems: Removal of textile dyes from wastewater in a microreactor
- Author
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Svetozarević Arsović, Milica, Šekuljica, Nataša, Dajić, Ana, Mihajlović, Marina, Marković, Maja, Knežević-Jugović, Zorica, Mijin, Dušan, Svetozarević Arsović, Milica, Šekuljica, Nataša, Dajić, Ana, Mihajlović, Marina, Marković, Maja, Knežević-Jugović, Zorica, and Mijin, Dušan
- Abstract
Wastewater from textile industry contains considerable amount of dissolved dye that can trigger environmental issues if is not treated properly. Numerous methods have been developed to degrade recalcitrant pollutants safely and utterly. Among them, enzymatic treatment of wastewater is gaining attention due to the enzyme’s specificity, easier manipulation, and generation of less toxic by-products. Still, the cost of enzymatic systems is the main limitation keeping the biocatalysts at lab-scale. Alternative solution for reducing the cost of enzymatic reaction systems is the use of microfluidic systems, which contribute to better mixing, process intensification and cleaner production. In this study, implementation of horseradish peroxidase for removal of the textile dye Acid Violet 109 is performed in a microfluidic reactor. The microreactor consists of three plunger pump units, two mixers and PTFE tube. The process parameters: residence time, dye, hydrogen peroxide, enzyme activity, the reactors’ diameter and length were optimized. Under the optimal process conditions: 30 mg/L dye concentration, 0.8 U/mL horseradish peroxidase activity, 0.1 mM hydrogen peroxide, 0.25 mm reactor's diameter, 97,3 % removal was achieved at residence time of 6 min. The results from this study show that enzymatic microfluidic reactors are a convenient technology for dye removal.
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- 2023
7. Flow in the tube: crosslinking of peroxidase from agricultural waste for dye removal in microtubular reactor
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Svetozarević, Milica, Šekuljica, Nataša, Marković, Maja, Dajić, Ana, Mihajlović, Marina, Mijin, Dušan, Svetozarević, Milica, Šekuljica, Nataša, Marković, Maja, Dajić, Ana, Mihajlović, Marina, and Mijin, Dušan
- Abstract
Agro-industrial waste has gained special attention as an abundatly available natural carbon source, which can be used for value-added products such as generating power, biofuel, biogas and biofertilizers production. Further application of agro-industrial waste may be as a substrate for solid state fermentation for production of antibiotics, enzymes, and phytochemicals. Isolation of enzymes from waste material as crude extracts contributes to the concept of sustainability and lowers their cost significantly. The enzyme of interest in this study was peroxidase isolated from soybean hull. Furthermore, the crude peroxidase from soybean hull was crosslinked by oxidized pectin onto the inner walls of PTFE microtubular reactor. The influence of the crosslinker concentration on the enzyme activity and crosslinking efficiency was evaluated, together with the effect of the reagents flow rate on the enzyme activity and crosslinking efficiency. Two different microtubular diameters were examined for the crude peroxidase crosslinking: 0.5 and 0.8 mm. After the immobilization, the possibility of anthraquinone dye removal by crosslinked peroxidase in the microtubular reactor was examined. The reusability of the immobilized enzyme was also evaluated in this study., Poljoprivredni otpad predstavlja lako dostupan prirodni izvor ugljenika, koji se može koristiti za dobijanje proizvoda sa dodatom vrednošću. Poljoprivredni otpad se može koristiti kao sirovina za proizvodnju električne energije, biogorivo, biogas i proizvodnju biođubriva. Dalja primena poljoprivrednog otpada može biti kao supstrat za fermentaciju u čvrstom stanju (eng. solid state fermentation), za proizvodnju antibiotika, enzima i fitohemikalija. Izolovanje enzima iz otpadnog materijala kao sirovi ekstrakt doprinosi konceptu održivosti i značajno smanjuje cenu enzima. Enzim od interesa u ovom radu je peroksidaza izolovana iz sojinih ljuspica. Sirova peroksidaza iz sojinih ljuspica umrežena je oksidovanim pektinom na unutrašnje zidove PTFE cevnog mikroreaktor, i ispitivan je uticaj koncentracije umreživača na aktivnost enzima i efikasnost umrežavanja, kao i uticaj protoka reagensa na aktivnost enzima i efikasnost umrežavanja. Nakon toga, vršeno je ispitivanje uticaja prečnika cevnog mikroreaktora na efikasnost umrežavanja i aktivnost peroksidaze: 0,5 i 0,8 mm. Nakon imobilizacije ispitana je mogućnost uklanjanja antrahinonske boje umreženom peroksidazom u cevnom mikroreaktoru. U ovom radu je takođe je ispitivana mogućnost ponovne upotrebe imobilisanog enzima.
- Published
- 2023
8. Novi postupak za biodegradaciju antrahinonskih boja u kontinualnom mikroreaktorskom sistemu peroksidazom izolovanom iz poljoprivrednog otpada
- Author
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Mijin, Dušan, Svetozarević, Milica, Šekuljica, Nataša, Knežević-Jugović, Zorica, Dajić, Ana, Mihajlović, Marina, Jovanović, Mića, Mijin, Dušan, Svetozarević, Milica, Šekuljica, Nataša, Knežević-Jugović, Zorica, Dajić, Ana, Mihajlović, Marina, and Jovanović, Mića
- Abstract
Predmetni pronalazak se odnosi na postupak biodegradacije antrahinonske boje Acid Violet 109 peroksidazom, izolovanom iz poljoprivrednog otpada u kontinualnom mikroreaktorskom sistemu. Postupak se sastoji od ekstrakcije enzima i biodegradacije boje u mikroreaktoru. Koncentracija boje treba da bude do 450 mg/L, enzimski ekstrakt aktivnosti 1 U/mL i vodonik-peroksid koncentracije do 1 mM, da bi se postigao najveći stepen biodegradacije. Na ovaj način dobija se visoko efikasan postupak biodegradacije antrahinonskih boja (~97 %) koji je održiv, ekološki i ekonomski opravdan., The present invention relates to a method for anthraquinone dye Acid Violet 109 biodegradation using peroxidase, isolated from agro-industrial waste in continuous microreactor system. The method consists of enzyme extraction and dye biodegradation in microreactor. Dye concentration should be up to 450 mg/L, enzyme extract with the activity of 1 U/mL and hydrogen peroxide concentration up to 1 mM, in order to achieve the highest level of biodegradation. In this way highly efficient method for anthraquinone dyes biodegradation is obtained (~97 %) which is sustainable, ecologically and economically justified.
- Published
- 2023
9. Iskorišćenje potencijala deponijskog gasa u slučaju parcijalnog zatvaranja deponije
- Author
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Dajić, Ana, Mihajlović, Marina, Svetozarević, Milica, Dajić, Ana, Mihajlović, Marina, and Svetozarević, Milica
- Abstract
Deponovanje otpada jedan je od elemenata integrisanog upravljanja otpadom. Valjano pro-jektovanje bi, između ostalih elemenata, obavezno moralo da podrazumeva i prikupljanje deponij-skog gasa. Deponijski gas (eng. Landfill gas –LFG) nastaje kao proizvod anaerobnog razlaganja biodegradabilnih komponenti ostataka komunalnog otpada na deponiji i prosečno sadrži 50-55% metana (CH4) i 45-50% ugljen-dioksida (CO2). Energetska vrednost deponijskog gasa kreće se u rasponu 46-30 MJ/m3. Pored energetskog potencijala deponijski gas je veoma važan sa stanovišta zaštite životne sredine i važno jeiskoristiti deponijski gas pre nego što se deponija potpuno zatvori. Rad govori o mogućnosti iskorišćenja potencijala deponijskog gasa pre njenog zatvaranja., Waste disposal is an important element of integrated waste management. It is a necessary step although it could significantly affect the environment and the goal is to do everything to protect it. Collection of landfill gas (LFG)should be included the in proper landfill design. Landfill gas, as a product of the anaerobic decomposition of biodegradable components from municipal waste at the landfill, contains on average 50-55% methane (CH4) and 45-50% carbon dioxide (CO2). The energy value of landfill gas ranges from 46 to 30 MJ/m3. The paper is about the possibility of utilizing the potential of landfill gas before its closure.
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- 2023
10. The effect of climate change on the potential for landfill gas generation at the Vinča landfill site
- Author
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Dajić, Ana, Mihajlović, Marina, Svetozarević Arsović, Milica, Dajić, Ana, Mihajlović, Marina, and Svetozarević Arsović, Milica
- Abstract
Climate change projections indicate that Republic of Serbia and the Western Balkans will face a high probability of continuing temperature increases, climatic extremes are projected to become more common, including a significant increase in the number of extreme heat events. Heavier precipitation events are expected in the winter months, whilst summers are projected to become even drier. This paper aims to analyse effect of precipitation changes on landfill gas generation on the Vinča landfill, the biggest landfill in Serbia. Quantities of generated landfill gas and methane have been estimated by using LandGem model. Site specific methane generation rate has been calculated according to GMI methodology for Central Eastern Europe. Sensitivity analyses is performed to determine influence of a precipitation regimes on the methane generation rate on the site. Any increase in precipitation (in range 10-30%) may increase landfill gas production for 4.3%. The paper shows the difference in gas emissions, with the same composition of waste, and different precipitation regimes. Changes in precipitation regimes due to climate change can affect the dynamics of landfill gas emissions. This information is significant for the possible re-circulation of leachate from the landfill which could result in an increased production of landfill gas.
- Published
- 2023
11. Landfill design in Serbia
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Karanac Milica, Jovanović Mića, Mihajlović Marina, Dajić Ana, Stevanović Dimitrije, and Jovanović Jovan
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landfill design ,waste disposal procedure ,impermeability ,landfill leachate ,landfill gas ,Environmental engineering ,TA170-171 - Abstract
Waste disposal is an important element of integrated waste management. In order to dispose of waste that is free of environmental risk, the proper design of landfills during their construction and/or closure is necessary. The first section of the paper presents the current state of landfills in Serbia, the second deals with problems in project design of landfills, especially in regard to their: a) program of waste disposal; b) impermeable layer; c) leaching collection and treatment; and d) gas collection and treatment. Analysis shows that many modern landfills in Serbia do not meet environmental protection requirements, therefore, they need reconstruction. All existing landfills owned by municipalities, as well as illegal dump sites, should be adequately closed. This paper presents the guidelines for successful landfill design which are to serve to meet the requirements and recommendations of Serbian and European regulations. Sound design of landfill technological elements should insure full sustainability of landfills in Serbia.
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- 2015
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12. Landfill design: need for improvement of water and soil protection requirements in EU Landfill Directive
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Dajić, Ana, Mihajlović, Marina, Jovanović, Mića, Karanac, Milica, Stevanović, Dimitrije, and Jovanović, Jovan
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- 2016
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13. KONTINUALNI SISTEM ZA OBEZBOJAVANJE OTPADNIH VODA. PRIMENA UMREŽENE PEROKSIDAZE IZ POLJOPRIVREDNOG OTPADA U UKLANJANJU BOJE.
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SVETOZAREVIĆ, Milica, ŠEKULJICA, Nataša, MARKOVIĆ, Maja, DAJIĆ, Ana, MIHAJLOVIĆ, Marina, and MIJIN, Dušan
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PEROXIDASE ,AGRICULTURAL wastes ,MICROREACTORS ,DYES & dyeing ,BIOFERTILIZERS - Abstract
Copyright of Proceedings of the International Congress on Process Engineering - Processing is the property of Union of Mechanical & Electrotechnical Engineers & Technicians of Serbia (SMEITS) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2023
14. Upotreba mikroreaktorskih sistema u procesima prečišćavanja otpadne vode
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Dajić, Ana, Mihajlović, Marina, Svetozarević, Milica, Dajić, Ana, Mihajlović, Marina, and Svetozarević, Milica
- Abstract
Porast industrijske aktivnosti za posledicu ima sve veću potrošnju vode a samim tim i sve veće količine otpadne vode opterećene značajnim količinama zagađujućih materija. U vreme kada je zaštita životne sredine aktuelna tema, tehnike prečišćavanja se neprestano unapređuju. Relativno nov koncept prerade otpadne vode uključuje tretmane u mikroreaktorskim sistemima kao atraktivnu alternativu standardnim procesima prečišćavanja. Kako postoji veliki broj različitih tipova mikroreaktorskih sistema i veliki broj metoda uklanjanja zagađujućih materija, otvaraju se nove mogućnosti primene različitih tretmana obrade. U radu je dat pregled tretmana industrijskih otpadnih voda koje je moguće primeniti u mikroreaktorskim sistemima., The increase in industrial activity results in increasing water consumption and increasing amounts of wastewater burdened with significant amounts of pollutants. Environmental protection has become an important topic and wastewater treatment techniques are constantly being improved. A relatively new concept includes treatments in microreactor systems as an attractive alternative to standard processes. Large number of different types of microreactor systems and large number of methods for removing pollutants opens up, new possibilities for the application of different treatment types. The paper gives an overview of industrial wastewater treatments that could be applied in microreactor systems with their advantages over classical processes.
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- 2022
15. Biorazgradnja antrahinonske boje peroksidazom izolovanom iz otpadnog materijala u šaržnom i kontinualnom sistemu
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Mijin, Dušan, Šekuljica, Nataša, Knežević-Jugović, Zorica, Popovski, Zoran T., Dajić, Ana, Svetozarević, Milica, Mijin, Dušan, Šekuljica, Nataša, Knežević-Jugović, Zorica, Popovski, Zoran T., Dajić, Ana, and Svetozarević, Milica
- Abstract
Ideja ove doktorske disertacije je izolovanje peroksidaze iz otpadnog materijala i njena upotreba urazgradnji antrahinonske boje Kiselo ljubičasto 109 (Acid Violet 109, AV109). Enzim je izolovaniz otpadnog materijala u cilju povećanje ekonomičnosti i održivosti procesa. Razmotreni su šaržni ikontinualni sistemi u cilju optimizacije razgradnje boje.U prvom delu disertacije ispitivana je mogućnost izolovanja peroksidaze iz različitog otpadnogmaterijala i odabir najpogodnijeg izvora. Potom, je izvršena optimizacija procesa razgradnje AV109u šaržnom reaktoru. Sa tim u vezi ispitan je uticaj procesnih parametara kao što su: vreme kontakta,pH reakcione smeše, koncentracija enzima, vodonik-peroksida i boje, i temperatura. Zatim, posebnapažnja je posvećena ispitivanju početne kinetike enzimske reakcije i utvrđivanju kinetičkihkonstanti.Sledeći deo disertacije odnosi se na kontinualnu razgradnju antrahinonske boje AV109 umikroreaktorskom sistemu. Kontinualni sistemi generalno pokazuju veću efikasnost, a olakšano je ivođenje procesa. U tu svrhu, optimizovani su parametri koji imaju glavnu ulogu u razgradnjiAV109 u mikroreaktoru: vreme zadržavanja, dužina i prečnik reaktora kao i koncentracijski protokvodonik-peroksida, enzima i boje.Da bi se čitav proces unapredio i postao efikasniji, ,,zeleniji” i ekonomičniji, vršena je imobilizacijaenzima umrežavanjem u kontinualnom i šaržnom reaktoru. Na taj način, bilo je moguće koristitienzim dobijen iz otpadnog materijala više puta. Ova vrsta imobilizacije odvija se u dva koraka:taloženje enzima i umrežavanje. Za umrežavanje enzima korišćen je pektin - prirodni polimerbezbedan za okolinu. Nakon odabira najpogodnijeg taložnog reagensa ispitivan je uticajkoncentracije umreživača na aktivnost enzima. Umreženi enzimski agregati su primenjeni u reakcijibiorazgradnje AV109 u šaržnom reaktoru zapremine 50 mL i optimizovani su sledeći parametri: pHvrednost, koncentracija imobilizata, vodonik-peroksida i boje. Takođe, ispitana je operativnastab, The idea behind this doctoral thesis is extraction of peroxidase from waste material and its use forbiodegradation of the anthraquinone dye Acid Violet 109. The enzyme is isolated from agro-industrial waste in order to increase the overall cost-effectiveness and sustainability of the process.Batch and continuous systems are considered for dye removal process’s optimization.In the thesis first section, the possibility of peroxidase extraction from different waste material andselection of the most suitable one is examined. Afterwards, optimization of the biodegradationbatch process is carried out. Contact time, pH, enzyme, hydrogen peroxide and dye concentration,temperature, kinetic constants and inhibition constants are optimized.Next section of this doctoral thesis deals with the continuous dye biodegradation in a microreactor.Continuous systems show increased efficiency and easier manipulation of the process. Bearing inmind the latter mentioned characteristics, the key parameters for continuous biodegradation of theAcid Violet 109 dye are optimized: residence time, reactor’s length and diameter, together withprocess parameters: concentration flow of enzyme, hydrogen peroxide and dye.As a means to a more advanced, efficient and greener process, the enzyme is immobilized by cross-linking in the batch and continuous reactor. Thus, the enzyme obtained from a waste material can bereused several times. Pectin is used for enzyme cross-linking. It is a biopolymer that is recognizedas safe for the environment. The effect of the precipitation reagents on the enzyme activity isexamined. After choosing the proper precipitant, the cross-linker concentration is optimized. Thecross-linked enzyme aggregates are applied in the biodegradation of the athraquinone dye in a 50mL batch reactor. The following parameters are optimized: pH, cross-linked enzyme, hydrogenperoxide and dye concentration. The possibility of the cross-linked enzyme aggregates reuse isconfirmed by biodegrading th
- Published
- 2022
16. To be free or not to be free: cross-linking of potato peel peroxidase for “green” degradation of anthraquinone dye
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Svetozarević, Milica, Šekuljica, Nataša, Dajić, Ana, Mihajlović, Marina, Knežević-Jugović, Zorica, Mijin, Dušan, Svetozarević, Milica, Šekuljica, Nataša, Dajić, Ana, Mihajlović, Marina, Knežević-Jugović, Zorica, and Mijin, Dušan
- Abstract
Enzyme immobilization is a convenient technique for reuse of enzymes –it is one of the advantages that contributes for enhanced productivity and efficacy of these processes. Enzyme stability and their recovery from a reaction mixture are just a couple of the many benefits that can be acquired by immobilization [1]. These features give opportunity for enzymatic application at industrial scale. The enzyme used in this study is perox-idase, an oxidoreductase that oxidizes variety of organic pollutants such as: phenols, textile dyes and phar-maceutically active compounds[2]. The use of potato peel as a source of peroxidase for degradation of textile dye contributes to the sustainability of the treatment. The simple isolation of peroxidase as a crude extract adds up to the cost effectiveness of the product (peroxidase).Given the fact that peroxidase is an eco-friendly biocatalyst, an effort is made to find a suitable ‘green’ way of immobilization. Glutaraldehyde is the most common cross-linker used for the same kind of immobilization [3]. However, it is a toxic compound, so it is replaced by alternative compounds such as natural polysaccharides. One of them is pectin that can be oxidized in order to introduce aldehyde groups, which in turn react with the amino-groups from the amino-acid residues and form Schiff bases [4], [5]. Afterwards, the cross-linked peroxidase can be applied for oxidation reaction.In this study, peroxidase was isolated from waste material –potato peel as a crude extract. The enzy-matic crude extract was precipitated with different reagents, in order to find the most suitable one. Next, the influence of the cross-linker concentration was examined. The cross-linked potato peel peroxidase was used for biodegradation of the anthraquinone dye Acid Violet 109. The process parameters: pH, reaction time, enzyme, hydrogen peroxide and dye concentration were optimized for achieving the maximal degradation rate. The operational stability, as a key parameter i, Boje koje se koriste u tekstilnoj industriji predstavljaju opasnost po životnu sredinu zbog toga što veći deo na kraju završi u vodotokovima. Kako konvencionalne tretmane karakteriše potreba za velikim količinama reagenasa, visoki troškovi procesa kao i pojava značajnih količina otpadnih materija na kraju procesa, stalno se radi na pronalasku novih efikasnijih tretmana otpadne vode. Za razliku od klasičnih tretmana, primena enzima, kao ekološki benignih biokatalizatora, predstavljaju „zeleno“ rešenje ovog problema. Kako bi se smanjili troškovi proizvodnje enzima predložena je njihova izolacija iz otpadnih materijala. Dugotrajni, komplikovani postupci prečišćavanja otpadnih materijala mogli bi se zaobići izolacijom u obliku sirovog ekstrakta. Imobilizacija enzima omogućila bi njihovu višekratnu upotrebu što bi doprinelo efikasnosti procesa. U izvedenim eksperimentima, ispitan je uticaj taložnog reagensa i koncentracije sredstva za umrežavanje na aktivnost enzima i efikasnost imobilizacije. Nakon umrežavanja, enzimski agregati su su korišćeni za razgradnju boje Acid Violet 109 i optimizovani su sledeći parametri: pH, koncentracija enzima, koncentracija vodonik-peroksida i koncentracija boje. Tokom eksperimenata ispitana je i operativna stabilnost umreženih agregartata.
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- 2022
17. Waste management strategies effects on GHG emission: Case study of Serbia
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Mihajlović, Marina, Dajić, Ana, Svetozarević, Milica, Mihajlović, Marina, Dajić, Ana, and Svetozarević, Milica
- Abstract
Landfilling is a dominant method of waste management in most developing countries. Moreover, a significant quantity of waste has been disposed on open dumps and unmanaged disposal sites. Landfill gas (LFG) can be considerable source of GHG emissions, as it consists of 50 % methane and 50 % carbon dioxide. Republic of Serbia is a developing country. Its waste management legislation is based on EU legislation. In the first decade of 2000s a set of laws was passed in this area. Unfortunately, the majority of goals were not fulfilled. An updated version of Waste management program is active since 2022. In Serbia, 2.95 million tons of waste is generated every year. It is estimated that 15-20 % is disposed on dumps, while 80 % is collected and disposed on the landfills. Waste treatment does not exist. Recycling rate is a very low, around 15%. Different waste management scenarios of impact on GHG emissions have been analysed. The analysis was performed using the software "Tool for Calculating Greenhouse Gases (GHG) in Solid Waste Management (SWM-GHG calculator)"developed by the German IFEU (ger. Institut für Energie- und Umweltforschung Heidelberg). The base scenario describes the current situation. Scenarios 1 and 2 describe goals set for 2025 and 2030 by Waste management program in the Republic of Serbia for the period 2022 - 2031. Scenario 3 describes best case scenario, with fully functional modern waste management system. The analysis includes several parameters, such as landfill type, recycling rate, LFG collection efficiency and use of waste incineration technology.
- Published
- 2022
18. Cross-linking the peroxidase: from potato peel valorization to colored effluents treatment
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Svetozarević, Milica, Šekuljica, Nataša, Dajić, Ana, Mihajlović, Marina, Popovski, Z., and Mijin, Dušan
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General Medicine ,General Chemistry - Abstract
Despite the obvious benefits from mass production such as an increased productivity, lower product cost and rapid evolution, nowadays we are dealing with energy consumption issues and pollution. The generated waste poses a threat to the environment, so new green techniques are developed constantly. Waste valorization is one of the trending concepts that is a part of sustainability strategies. Potato exploitation due to mass production of chips, hash browns, frozen food and starch leads to a formation of high waste load. There are several available ways for potato peel valorization: as a biofertilizer, as a substrate for microbial growth, as an adsorbent, for extraction of antioxidants and for extraction of enzymes. The enzyme peroxidase is abundant in potato peel. This enzyme uses hydrogen peroxide as an activator and can be readily used for oxidation of different compounds - pollutants. In this study, peroxidase was isolated from potato peel and immobilized as cross-linked enzyme aggregates. Pectin was used as a green cross-linker. The immobilized potato peel peroxidase was used for degradation of a textile anthraquinone dye Lanaset Violet B. Under the optimal process parameters: pH 3, 0.4 mM hydrogen peroxide, 0.8 μmol/min CLEA peroxidase, 10 mg/L dye and 70 min, 85.71±1.45 % dye degradation was achieved. The operational stability, as a key parameter for immobilized enzyme systems, was also examined. After 4 cycles CLEA peroxidase kept 31.57±1.79 % of its biodegradation efficiency.
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- 2022
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19. Landfill closure best available technique development: case study of Serbia
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Dajić, Ana, Jovanovic, M., Mihajlovic, M., Dajić, Ana, Jovanovic, M., and Mihajlovic, M.
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Waste disposal is the last but inevitable step in waste management and it significantly effects the environment. According to the Serbian Waste Management Strategy, disposal sites should be closed and new, sanitary regional landfills should be established. In this paper, case study method has been used to analyse technical regulations in order to determine the need for more detailed elaboration of the Best available technique (BAT) for landfill closure. Based on the analyses results, a number of proposals have been formulated and elaborated aimed to ensure pollution prevention and environment protection on the old and new landfills. A new national model for improvement BAT landfills closure is proposed. Proposed BAT improvements for landfill cover might be used on the international level, because many countries do not have BAT elaborations beside the Landfill Directive.
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- 2020
20. Razvoj procesa završnog tretmana čvrstih i tečnih zagađujućih materija primenom principa čistije proizvodnje
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Dajić, Ana, Jovanović, Mića, Petrović, Slobodan, Glišić, Sandra, Mihajlović, Marina, and Adžić, Miroljub
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landfill technological design ,azo dyes ,microreactor systems ,tehnološko projektovanje deponija ,koeficijent vodopropusnosti ,mikroreaktorski sistemi ,evropska Direktiva o deponijama ,azo boje ,obezbojavanje ,Council Directive 1999/31/EC on the landfill ,otpadna voda ,decolorisation ,wastewater ,water permeability coefficient - Abstract
Istraživanja u okviru ove doktorske disertacije sprovedena su sa ciljem razvoja procesa završnog tretmana čvrstih i tečnih zagađujućih materija primenom principa čistije proizvodnje. Pri projektovanju tehnoloških rešenja, procesa i postrojenja, u funkciji zaštite životne sredine, moguće je i neophodno koristiti principe i metod čistije proizvodnje. Istraživanja iz oblasti unapređenja tehnologije završnog tretmana čvrstih zagađujućih supstanci izvedena su na primeru odlaganja otpada dok su istraživanja iz oblasti unapređenja tehnologije završnog tretmana tečnih zagađujućih supstanci izvedena na primeru prečišćavanja simulirane otpadne vode iz tekstilne industrije... The main goal of the research conducted within this doctoral dissertation was final treatment processes development for solid and liquid pollutants applying cleaner production principles. Aiming to prevent pollution and protect environment applying of cleaner production in processes design have main role. Research in field of technological design development for treatment of solid waste materials was done on example of waste landfiling. In case of liquid waste materials treatment, research was done for simulated wastewater from textile industry...
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- 2019
21. Development of final treatment processses for solid and liquid pollutants by cleaner production principles application
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Dajić, Ana, Jovanović, Mića, Petrović, Slobodan, Glišić, Sandra, Mihajlović, Marina, and Adžić, Miroljub
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landfill technological design ,azo dyes ,microreactor systems ,tehnološko projektovanje deponija ,koeficijent vodopropusnosti ,mikroreaktorski sistemi ,evropska Direktiva o deponijama ,azo boje ,Council Directive 1999/31/EC on the landfill ,obezbojavanje ,otpadna voda ,decolorisation ,wastewater ,water permeability coefficient - Abstract
The main goal of the research conducted within this doctoral dissertation was final treatment processes development for solid and liquid pollutants applying cleaner production principles. Aiming to prevent pollution and protect environment applying of cleaner production in processes design have main role. Research in field of technological design development for treatment of solid waste materials was done on example of waste landfiling. In case of liquid waste materials treatment, research was done for simulated wastewater from textile industry... Istraživanja u okviru ove doktorske disertacije sprovedena su sa ciljem razvoja procesa završnog tretmana čvrstih i tečnih zagađujućih materija primenom principa čistije proizvodnje. Pri projektovanju tehnoloških rešenja, procesa i postrojenja, u funkciji zaštite životne sredine, moguće je i neophodno koristiti principe i metod čistije proizvodnje. Istraživanja iz oblasti unapređenja tehnologije završnog tretmana čvrstih zagađujućih supstanci izvedena su na primeru odlaganja otpada dok su istraživanja iz oblasti unapređenja tehnologije završnog tretmana tečnih zagađujućih supstanci izvedena na primeru prečišćavanja simulirane otpadne vode iz tekstilne industrije...
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- 2019
22. Razvoj procesa završnog tretmana čvrstih i tečnih zagađujućih materija primenom principa čistije proizvodnje
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Jovanović, Mića, Petrović, Slobodan, Glišić, Sandra, Mihajlović, Marina, Adžić, Miroljub, Dajić, Ana, Jovanović, Mića, Petrović, Slobodan, Glišić, Sandra, Mihajlović, Marina, Adžić, Miroljub, and Dajić, Ana
- Abstract
Istraživanja u okviru ove doktorske disertacije sprovedena su sa ciljem razvoja procesa završnog tretmana čvrstih i tečnih zagađujućih materija primenom principa čistije proizvodnje. Pri projektovanju tehnoloških rešenja, procesa i postrojenja, u funkciji zaštite životne sredine, moguće je i neophodno koristiti principe i metod čistije proizvodnje. Istraživanja iz oblasti unapređenja tehnologije završnog tretmana čvrstih zagađujućih supstanci izvedena su na primeru odlaganja otpada dok su istraživanja iz oblasti unapređenja tehnologije završnog tretmana tečnih zagađujućih supstanci izvedena na primeru prečišćavanja simulirane otpadne vode iz tekstilne industrije..., The main goal of the research conducted within this doctoral dissertation was final treatment processes development for solid and liquid pollutants applying cleaner production principles. Aiming to prevent pollution and protect environment applying of cleaner production in processes design have main role. Research in field of technological design development for treatment of solid waste materials was done on example of waste landfiling. In case of liquid waste materials treatment, research was done for simulated wastewater from textile industry...
- Published
- 2019
23. Development of final treatment processses for solid and liquid pollutants by cleaner production principles application
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Jovanović, Mića, Petrović, Slobodan, Glišić, Sandra, Mihajlović, Marina, Adžić, Miroljub, Dajić, Ana, Jovanović, Mića, Petrović, Slobodan, Glišić, Sandra, Mihajlović, Marina, Adžić, Miroljub, and Dajić, Ana
- Abstract
The main goal of the research conducted within this doctoral dissertation was final treatment processes development for solid and liquid pollutants applying cleaner production principles. Aiming to prevent pollution and protect environment applying of cleaner production in processes design have main role. Research in field of technological design development for treatment of solid waste materials was done on example of waste landfiling. In case of liquid waste materials treatment, research was done for simulated wastewater from textile industry..., Istraživanja u okviru ove doktorske disertacije sprovedena su sa ciljem razvoja procesa završnog tretmana čvrstih i tečnih zagađujućih materija primenom principa čistije proizvodnje. Pri projektovanju tehnoloških rešenja, procesa i postrojenja, u funkciji zaštite životne sredine, moguće je i neophodno koristiti principe i metod čistije proizvodnje. Istraživanja iz oblasti unapređenja tehnologije završnog tretmana čvrstih zagađujućih supstanci izvedena su na primeru odlaganja otpada dok su istraživanja iz oblasti unapređenja tehnologije završnog tretmana tečnih zagađujućih supstanci izvedena na primeru prečišćavanja simulirane otpadne vode iz tekstilne industrije...
- Published
- 2019
24. Improvement of the Textile Industry Wastewater Decolorization Process Using Capillary Microreactor Technology
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Dajić, Ana, Mihajlović, Marina, Mandić-Rajcević, Stefan, Mijin, Dušan, Jovanović, Mica, Jovanović, Jovan, Dajić, Ana, Mihajlović, Marina, Mandić-Rajcević, Stefan, Mijin, Dušan, Jovanović, Mica, and Jovanović, Jovan
- Abstract
Dyes are an important class of pollutants because large amounts are often found in the environment as a result of their extensive industrial use. Traditional wastewater treatment methods often lead to high energy costs, formation of by-products, and the production of sludge. This paper analyzes the possibility of using a new, sustainable approach to water decolorization with reduced consumption of chemicals. In microreactor experiments, reactant molar ratios, volumetric flow rate, and microreactor length and diameter were varied. The obtained results showed that batch decolorization required 250-500 times higher molar ratios to achieve comparable decolorization (70-90%) and at least three times longer residence time. Microreactor experiments demonstrated that higher microreactor lengths and molar ratios influence positively the decolorization process, although satisfactory results are also achieved with medium microreactor lengths and lower molar ratios. Higher fluids velocities contribute to the decolorization process, but the best results were obtained using a medium velocity (in a 5.8m microreactor system) to achieve the highest possible mixing intensity and long enough residence time. Microreactor systems have achieved significantly better decolorization results, considering any combination of microreactor length, molar ratio, diameter, flow rate, and residence time, than a batch system. Thus, a medium length microreactor system, with a low concentration of NaOCl, low flow rate, small diameter, and medium residence time can achieve satisfactory decolorization results, but with a lower consumption of chemicals, energy, equipment, and better environmental impact. Article HighlightsColored wastewater was decolorized using batch and microreactor systems.Various microreactor lengths, diameters, and mixture velocities were tested.Microreactor system required 500 times lower amounts of decolorizing agent.
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- 2019
25. Development of Green Chemical Process: The Reaction of Condensation in a Continuous Flow Microreactor System
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Tadić, Julijana, Svetozarević, Milica, Dajić, Ana, Mihajlović, Marina, Jovanović, Mića, Mijin, Dušan, Tadić, Julijana, Svetozarević, Milica, Dajić, Ana, Mihajlović, Marina, Jovanović, Mića, and Mijin, Dušan
- Abstract
Aromatic heterocyclic compounds containing nitrogen in their structure have attracted considerable interest in medicine, because of their certain pharmacological properties including anticancer, anti-inflammatory, antihypertensive, antibacterial, antifungal and antiviral activities. Among them, derivatives with 2-pyridone moiety are particularly notable due to their wide application in pharmacological and dye industry. A usual procedure for synthesis of 2-pyridone moiety is classic condensation reaction of 1,3- dicarbonyl compounds with cyanoacetamide. However, in the case of various substituted reactants, drawback of this reaction can be a low yield of desirable products and long reaction time. This factor has led to the development of different methods for synthesis in order to overcome these defects. The microreactor technology and flow chemistry could play a significant role in the improving of organic synthesis efficiency. The microreactor approach could provide enhanced productivity, fast conversion and altogether less hazardous chemical synthesis. Furthermore, it pushes towards designing of chemistry with a lower environmental and economic impact. In this study the synthesis of 2-pyridone moiety in a continuous flow coil-type microreactor system was examined. In order to optimize reaction conditions, flow rates of starting reagents were varied. The desirable 2-pyridone was obtained in satisfactory yield of circa 60% in less than 10 minutes. The structure and purity of synthetized 2-pyridone moiety were confirmed by melting point, FT-IR, 1 H NMR and UV-Vis spectra. The efficiency of the presented method for the synthesis of 2-pyridone based molecules has promising scaleup potential.
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- 2019
26. Are the Tube Microreactors Future of Wastewater Treatment?
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Dajić, Ana, Mihajlović, Marina, Svetozarević, Milica, Tadić, Julijana, Jovanović, Mića, Dajić, Ana, Mihajlović, Marina, Svetozarević, Milica, Tadić, Julijana, and Jovanović, Mića
- Abstract
In the industry, wastewater is important environmental problem. Due to the growing environmental concern this problem became one of key issues. Large amounts of highly contaminated wastewater, as consequence of industrial use, are important environmental polluters. Before discharging, wastewater has to be treated to achieve quality prescribed by law. One of solutions could be decomposition of pollutants. The decomposition of pollutants from industrial wastewaters is of critical importance in order to maintain a clean environment. Many process commonly used in polluter degradation processes commonly use different harmful chemicals or leads to formation of potentially harmful byproducts. Treatment of wastewater from industry in microreactor system is new ecological solution. Over the last few decades microreactors have been increasingly recognized as being an alternative to conventional batch reactors in chemical industry. Application of these systems have aim to transform conventional into more productive and environmental friendlier process. They are characterized by micrometer dimensions and continuous mode, resulting in significant intensification of the mass and heat transfer. Microreactor systems are already find application in chemical industry, biotechnology, pharmaceutical industry and medicine. Decomposition of pollutants in wastewater is complex, but microreactors allow purification with low quantities of chemical reagents. This paper represents review of possible fields of wastewater purification – waste material decomposition in microreactor systems.
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- 2019
27. Advantages of Microreactor Technology over Conventional Methods in Enzymatic Wastewater Treatment – Environmental Application of Enzymes
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Svetozarević, Milica, Tadić, Julijana, Mihajlović, Marina, Dajić, Ana, Jovanović, Mića, Svetozarević, Milica, Tadić, Julijana, Mihajlović, Marina, Dajić, Ana, and Jovanović, Mića
- Abstract
Wastewater treatment is one of the most analysed issue in theory and practice because of its current and vast significance nowadays. Wide range of chemical, physical and biochemical processes are developed for wastewater treatment. Although biochemical processes are the most eco-friendly when compared to chemical and physical, they are still not the leading type of technology in this field. In recent years, microreactors are trending because of their particular characteristics that can be utilized in numerous areas. Continuous flow, higher reaction yields, scale independant and high selectivity are just a few of the beneficial characteristics of microreactors. Due to the micro channels, turbulent flow regime is avoided. Furthermore, high surface-to-area ratios aid in far greater and faster heat and mass transfer than in common reactors. Another asset of microreactors is short diffusion pathways, that together with small reaction pathways add to its favorable attributes. When it comes to enzymes, due to their small size and mild reaction conditions requirements, they are ideal for working in microreactos. Enzymes can be isolated from different sources, but isolation from plant waste material is what makes them even more interesting. Because of the above mentioned microreactor’s pros, several enzyme’s disadvantagious properties are overcome. In this study, a review of the positive characteristic of enzyme microreators in wastwater treatment will be discussed.
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- 2019
28. Radio „na oblaku“: novi autorski pristupi u kreiranju internet radija
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Dajić, Ana, primary and Radivojević, Sonja, primary
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- 2019
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29. Ispitivanje mogućnosti uklanjanja sintetskih boja u mikroreaktorskim sistemima
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Dajić, Ana, primary, Mihajlović, Marina, primary, Mijin, Dušan, primary, Jovanović, Jovan, primary, and Jovanović, Mića, primary
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- 2017
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30. Moderan pristup organskoj sintezi upotrebom mikroreaktorskih sistema
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Tadić, Julijana, primary, Dajić, Ana, primary, Matović, Luka, primary, Jovanović, Jovan, primary, Mihajlović, Marina, primary, Mijin, Dušan, primary, and Jovanović, Mića, primary
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- 2017
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31. Landfill design in Serbia
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Karanac, Milica, Jovanović, Mića, Mihajlović, Marina, Dajić, Ana, Stevanović, Dimitrije, Jovanović, Jovan, Karanac, Milica, Jovanović, Mića, Mihajlović, Marina, Dajić, Ana, Stevanović, Dimitrije, and Jovanović, Jovan
- Abstract
Waste disposal is an important element of integrated waste management. In order to dispose of waste that is free of environmental risk, the proper design of landfills during their construction and/or closure is necessary. The first section of the paper presents the current state of landfills in Serbia, the second deals with problems in project design of landfills, especially in regard to their: a) program of waste disposal; b) impermeable layer; c) leaching collection and treatment; and d) gas collection and treatment. Analysis shows that many modern landfills in Serbia do not meet environmental protection requirements, therefore, they need reconstruction. All existing landfills owned by municipalities, as well as illegal dump sites, should be adequately closed. This paper presents the guidelines for successful landfill design which are to serve to meet the requirements and recommendations of Serbian and European regulations. Sound design of landfill technological elements should insure full sustainability of landfills in Serbia., Deponovanje otpada je element integrisanog upravljanja otpadom. Valjano izrađeni projekti izgradnje i/ili zatvaranja deponije osiguravaju bezbedno odlaganje otpada, uz minimalne negativne uticaje na životnu sredinu. U prvom delu rada izvršen je pregled i analiza deponija u Republici Srbiji. U drugom delu rada, u funkciji analize, opisani su značajni elementi tehnološkog projektovanja savremenih deponija koji uključuju: a) program deponovanja, b) obezbeđivanje vodonepropusnosti dna i prekrivnog sloja, c) sakupljanje i tretman procednih voda i d) sakupljanje, tretman i iskorišćenje deponijskog gasa. U radu je konstatovano da postojeće, savremene deponije nisu u potpunosti projektovane u skladu sa propisima, te da je neophodna njihova rekonstrukcija. Takođe, postojeća smetilišta i divlje deponije neophodno je zatvoriti i sanirati. U ovom radu predstavljene su smernice za valjano tehnološko projektovanje deponija koje su u funkciji ispunjavanja zahteva i preporuka nacionalnih i evropskih propisa.
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- 2015
32. Landfill design: need for improvement of water and soil protection requirements in EU Landfill Directive
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Dajić, Ana, primary, Mihajlović, Marina, additional, Jovanović, Mića, additional, Karanac, Milica, additional, Stevanović, Dimitrije, additional, and Jovanović, Jovan, additional
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- 2015
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33. Biorazgradnja antrahinonske boje peroksidazom izolovanom iz otpadnog materijala u šaržnom i kontinualnom sistemu
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Svetozarević, Milica, Mijin, Dušan, Šekuljica, Nataša, Knežević-Jugović, Zorica, Popovski, Zoran T., and Dajić, Ana
- Subjects
peroksidaza iz sojinih ljuspica, peroksidaza iz krompirovih ljuski, otpadni material, biorazgradnja, antrahinonska boja, mikroreaktori, imobilizacija, oksidovani pektin, umreženi enzimski agregati, umreženi enzimi, proizvodi razgradnje ,soybean hull peroxidase, potato peel peroxidase, anthraquinone dye, waste material, immobilization, oxidized pectin, cross-linked enzyme aggregates, cross-linked enzymes, biodegradation products - Abstract
Ideja ove doktorske disertacije je izolovanje peroksidaze iz otpadnog materijala i njena upotreba u razgradnji antrahinonske boje Kiselo ljubičasto 109 (Acid Violet 109, AV109). Enzim je izolovan iz otpadnog materijala u cilju povećanje ekonomičnosti i održivosti procesa. Razmotreni su šaržni i kontinualni sistemi u cilju optimizacije razgradnje boje. U prvom delu disertacije ispitivana je mogućnost izolovanja peroksidaze iz različitog otpadnog materijala i odabir najpogodnijeg izvora. Potom, je izvršena optimizacija procesa razgradnje AV109 u šaržnom reaktoru. Sa tim u vezi ispitan je uticaj procesnih parametara kao što su: vreme kontakta, pH reakcione smeše, koncentracija enzima, vodonik-peroksida i boje, i temperatura. Zatim, posebna pažnja je posvećena ispitivanju početne kinetike enzimske reakcije i utvrđivanju kinetičkih konstanti. Sledeći deo disertacije odnosi se na kontinualnu razgradnju antrahinonske boje AV109 u mikroreaktorskom sistemu. Kontinualni sistemi generalno pokazuju veću efikasnost, a olakšano je i vođenje procesa. U tu svrhu, optimizovani su parametri koji imaju glavnu ulogu u razgradnji AV109 u mikroreaktoru: vreme zadržavanja, dužina i prečnik reaktora kao i koncentracijski protok vodonik-peroksida, enzima i boje. Da bi se čitav proces unapredio i postao efikasniji, ,,zeleniji” i ekonomičniji, vršena je imobilizacija enzima umrežavanjem u kontinualnom i šaržnom reaktoru. Na taj način, bilo je moguće koristiti enzim dobijen iz otpadnog materijala više puta. Ova vrsta imobilizacije odvija se u dva koraka: taloženje enzima i umrežavanje. Za umrežavanje enzima korišćen je pektin - prirodni polimer bezbedan za okolinu. Nakon odabira najpogodnijeg taložnog reagensa ispitivan je uticaj koncentracije umreživača na aktivnost enzima. Umreženi enzimski agregati su primenjeni u reakciji biorazgradnje AV109 u šaržnom reaktoru zapremine 50 mL i optimizovani su sledeći parametri: pH vrednost, koncentracija imobilizata, vodonik-peroksida i boje. Takođe, ispitana je operativna stabilnost umreženih enzimskih agregata. U kontinualnom sistemu, je ispitivana mogućnost imobilizacije enzima na unutrašnjim zidovima mikroreaktora. Imobilizacija peroksidaze u cevnom mikroreaktoru je potvrđena SEM analizom. Dalje, optimizovana je koncentracija umreživača i koncentracija enzima, a ispitivana je i operativna stabilnost u reakciji razgradnje boje AV109. Dobijeni rezultati ukazuju na efikasnu razgradnje boje, ali mehanizam delovanja peroksidaze nije rasvetljen u potpunosti. Zbog toga, izvršena je LC/GC – MS analiza proizvoda razgradnje. Ispitivana je i hemijska potrošnja kiseonika u cilju utvrđivanja toksičnosti sekundarnih proizvoda reakcije. The idea behind this doctoral thesis is extraction of peroxidase from waste material and its use for biodegradation of the anthraquinone dye Acid Violet 109. The enzyme is isolated from agro- industrial waste in order to increase the overall cost-effectiveness and sustainability of the process. Batch and continuous systems are considered for dye removal process’s optimization. In the thesis first section, the possibility of peroxidase extraction from different waste material and selection of the most suitable one is examined. Afterwards, optimization of the biodegradation batch process is carried out. Contact time, pH, enzyme, hydrogen peroxide and dye concentration, temperature, kinetic constants and inhibition constants are optimized. Next section of this doctoral thesis deals with the continuous dye biodegradation in a microreactor. Continuous systems show increased efficiency and easier manipulation of the process. Bearing in mind the latter mentioned characteristics, the key parameters for continuous biodegradation of the Acid Violet 109 dye are optimized: residence time, reactor’s length and diameter, together with process parameters: concentration flow of enzyme, hydrogen peroxide and dye. As a means to a more advanced, efficient and greener process, the enzyme is immobilized by cross- linking in the batch and continuous reactor. Thus, the enzyme obtained from a waste material can be reused several times. Pectin is used for enzyme cross-linking. It is a biopolymer that is recognized as safe for the environment. The effect of the precipitation reagents on the enzyme activity is examined. After choosing the proper precipitant, the cross-linker concentration is optimized. The cross-linked enzyme aggregates are applied in the biodegradation of the athraquinone dye in a 50 mL batch reactor. The following parameters are optimized: pH, cross-linked enzyme, hydrogen peroxide and dye concentration. The possibility of the cross-linked enzyme aggregates reuse is confirmed by biodegrading the dye in 10 successive cycles. In continuous flow, the peroxidase is cross-linked onto the inner walls of the PTFE tube microreactor. Scanning electron microscopy analysis confirmed the successful outcome of the immobilization. Afterwards, the enzyme and cross-linker concentration was optimized, together with reusability assessment of the immobilized enzyme microreactor. The obtained results indicate of a successful prosperous dye biodegradation process, but the mechanism of the biodegradation is not revealed. Hence, LC/GC-MS analysis of the degradation products is performed. Chemical oxygen demand was analyzed as a validation that generation of secondary byproducts which are more toxic than the dye itself does not occur.
- Published
- 2022
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