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Individual-specific mate preferences are thought to be widespread, but they are still poorly understood in terms of mechanisms and function. Earlier work on a songbird (the zebra finch, Taeniopygia guttata) showed predominantly individual-specific mate preferences and signs of behavioural incompatibility with certain partners. However, the phenotypic target of preference or aversion remains unclear. A previous study suggested that female preferences may be related to variation in male song. Male zebra finches have individually distinct songs and together with individual-specific female song preferences they could function according to a ‘key–lock principle’. Here we report on a preregistered study in which we tested the hypothesis that individual females respond to males with highly similar songs in a similar way. We selected 18 duos of males with nearly identical songs and released them with females in communal breeding aviaries. We scored female preference as the female's responsiveness to male courtship and confirmed that females indeed had strong individual-specific mate preferences. However, female responsiveness scores were not positively correlated across males closely matched for their song, thereby rejecting the idea of a song-based ‘key–lock principle’ as an explanation for the observed individual-specific preferences. We suggest researchers should focus on details of the interactions between potential partners to elucidate the cause of behavioural incompatibility.

Parental care in birds consists of many elaborate forms, including nest building, incubation, and offspring provision, but we still do not know how much each parent contributes to the different forms. Furthermore, the variations, relationships, and potential drivers of sex differentiation in providing care across different care stages remain largely unknown. Here, we surveyed species in birds and uncovered remarkable differences in the sex role patterns across different care forms. This result implied that parental care should not be treated as a unitary trait but as a composite of integrated features with great variations. Further analyses revealed moderate correlations of the sex roles between care forms, indicating the existence of shared intrinsic drivers. We tested the effects of sexual selection, certainty of paternity, predation risk, and offspring’s life history traits in driving sex role variations. Results showed that species with strong sexual selection on males or uncertainty of paternity tend to have female-biased care.

Colorful plumage ornaments may evolve because they play a role in mate choice or in intrasexual competition, acting as signals of species identity or of individual quality. The zebra finch (Taeniopygia guttata) is a model organism for the study of mate choice and its colorful plumage ornaments are thought to be used in both of these contexts. Numerous genetic color variants have been described for this species, but they are rare in the wild. This raises the question whether discrimination against deviant phenotypes maintains the species' uniform plumage color (rare-mate disadvantage). Furthermore, comparison to closely related species suggests that the lack of colorful ornaments in female zebra finches is a derived condition. Male preferences for less-ornamented females may have led to sexual dichromatism in the zebra finch. Here, we test the role of plumage ornaments experimentally by altering male and female coloration to mimic 2 types of naturally occurring genetic color variants. We estimated effects on social dominance and reproductive success in large breeding aviaries in one domesticated and 2 recently wild-derived populations. Hypotheses, methods, and analyses were preregistered to ensure maximal objectivity of the results presented. Despite a fairly drastic manipulation and a powerful experimental design, we found no effect of the treatment on social dominance or on reproductive success. Our results suggest that mate choice is not the mechanism that maintains homogeneity of zebra finch plumage coloration, or that can explain the loss of ornaments in females.

Parental care in birds usually consists of many elaborate forms, including nest building, incubation, provisioning the offspring and protecting them. Given the various life history differences between sexes, parents may have different opportunity costs of providing care in a specific form. But we still do not know whether males and females generally differ in their involvement in different care forms across stages of a breeding cycle, such as nest building, incubation and chick provisioning. Here, we performed a survey of parental care regarding which sex provides care in 882 species of passerine birds and found significant differences in the frequency distributions of sex-role patterns (i.e. female-only care, biparental care, and male-only care) across three distinct forms of parental care. This result showed clearly that parental care should not be treated as a unitary trait, but a composite of integrated features with diverse functions. Using a set of linear mixed-effect models we tested the effects of sexual selection, certainty of paternity, predation risk, and offspring9s life history traits in driving the variation in sex roles. In general, we found species with female-only care tended to be under strong sexual selection on males, and uncertainty of paternity could reduce male care.

A major challenge in behavior and evolutionary ecology is to understand the evolution and maintenance of animal personality. Theory suggests that females can benefit by choosing a high-quality mate, but largely ignores the potential interaction between male and female personality during mate choice. Here, we examined the influence of exploration on mate choice by captive female Java sparrows (Lonchura oryzivora). Females preferred high exploratory males as mates rather than choosing mates according to their own exploration, and thus showed no assortative mating. Our results highlight the role of exploration of males in the mate preference of birds and suggest that mate compatibility plays minor role in the mate preference.

Female mate choice is thought to be responsible for the evolution of many extravagant male ornaments and displays, but the costs of being too selective may hinder the evolution of choosiness. Selection against choosiness should be strongest in socially monogamous mating systems, because females may end up without a partner and forego reproduction, especially when many females prefer the same few partners (frequency-dependent selection). Here we quantify the fitness costs of having mating preferences that are difficult to satisfy. We capitalise on the recent discovery that female zebra finches (Taeniopygia guttata) prefer males of familiar song dialect. We measured female fitness in captive breeding colonies in which one third of females were given ample opportunity to choose a mate of their preferred dialect (two thirds of all males; ‘relaxed competition’), while two thirds of the females had to compete over a limited pool of mates they preferred (one third of all males; ‘high competition’). As expected, social pairings were strongly assortative with regard to song dialect. In the high-competition group, 26% of the females remained unpaired, yet they still obtained relatively high fitness by using brood parasitism as an alternative reproductive tactic. Another 31% of high-competition females paired disassortatively for song dialect. These females showed increased levels of extra-pair paternity, mostly with same-dialect males as sires, suggesting that preferences were not abolished after social pairing. However, females that paired disassortatively for song dialect did not have lower reproductive success. Overall, females in the high-competition group reached equal fitness as those that experienced relaxed competition. Our study suggests that alternative reproductive tactics such as egg dumping can help overcome the frequency-dependent costs of being highly selective in a monogamous mating system, thereby facilitating the evolution of female choosiness.

Culturally transmitted communication signals – such as human language or bird song – can change over time through a process of cultural drift, and may consequently enhance the separation of populations, potentially leading to reproductive isolation1–4. Local song dialects have been identified in bird species with relatively simple songs where individuals show high cultural conformity5–10. In contrast, the emergence of cultural dialects has been regarded as unlikely11–13for species with more variable song, such as the zebra finch (Taeniopygia guttata). Instead, it has been proposed that selection for individual recognition and distinctiveness may lead to a complete spread across the space of acoustic and syntactical possibilities11–15. However, another possibility is that analytical limitations have meant that subtle but possibly salient group differences have not yet been discovered in such species. Here we show that machine learning can distinguish the songs from multiple captive zebra finch populations with remarkable precision, and that these ‘cryptic song dialects’ drive strong assortative mating in this species. We studied mating patterns across three consecutive generations using captive populations that have evolved in isolation for about 100 generations. Cross-fostering eggs within and between these populations and quantifying social interactions of the resulting offspring later in life revealed that mate choice primarily targets cultural traits that are transmitted during a short developmental time window. Detailed social networks showed that females preferentially approached males whose song resembled that of their adolescent peers. Our study shows that birds can be surprisingly sensitive to cultural traits for mating that have hitherto remained cryptic, even in this well-studied species that is used as a model for song-learning13,14,16–28.

Moulting strategies in birds have evolved to avoid overlap with, or prepare for, other demanding parts of the annual cycle, such as reproduction or migration. When moulting for the first time after leaving the nest, young birds replace their typically poor‐quality plumage during the post‐juvenile moult. The extent of this moult varies between species from partial to complete. Earlier studies, restricted to Western Palearctic birds, suggest that in most species a complete post‐juvenile moult may not be possible simply because young birds are constrained by not having the same access to resources as adults, unless environmental conditions are favourable. These studies also show that complete post‐juvenile moult is more common in species with poor‐quality nest‐grown plumage. We expanded the spatial and taxonomic scope of previous studies to 1,315 species of passerines from across the world and considered both the role of constraints, plumage quality and other selective pressures favouring a complete post‐juvenile moult. Thus, we test whether complete moult is more prevalent in species where nest‐grown feathers are presumably of poor quality (shorter nestling period), that live in environments that foster quick plumage degradation (open habitats, high insolation and humidity), and where males are under strong sexual selection. Our data reveal that 24% of species carry out a complete post‐juvenile moult, and that this trait has a strong phylogenetic signal. Complete moult is more common in species that inhabit warmer regions and open habitats, show no delayed plumage maturation and have higher levels of sexual dichromatism (indicative of strong sexual selection). Neither the presumed quality of the nest‐grown plumage nor living in regions with high insolation correlates with complete moult. In conclusion, the evolution of complete post‐juvenile moult not only depends on whether birds can perform a complete moult (i.e. suitable environmental conditions) but also on the strength of selection associated with the need of a complete moult. In particular, the necessity to keep the plumage in good condition in challenging environments and the benefits associated with producing adult‐like plumage colours to attract mates or deter rivals seem to play an important role. Moulting strategies in birds have evolved to avoid overlap with, or prepare for, other demanding parts of the annual cycle, such as reproduction or migration. When moulting for the first time after leaving the nest, young birds replace their typically poor‐quality plumage during the post‐juvenile moult. The extent of this moult varies between species from partial to complete. Earlier studies, restricted to Western Palearctic birds, suggest that in most species a complete post‐juvenile moult may not be possible simply because young birds are constrained by not having the same access to resources as adults, unless environmental conditions are favourable. These studies also show that complete post‐juvenile moult is more common in species with poor‐quality nest‐grown plumage. We expanded the spatial and taxonomic scope of previous studies to 1,315 species of passerines from across the world and considered both the role of constraints, plumage quality and other selective pressures favouring a complete post‐juvenile moult. Thus, we test whether complete moult is more prevalent in species where nest‐grown feathers are presumably of poor quality (shorter nestling period), that live in environments that foster quick plumage degradation (open habitats, high insolation and humidity), and where males are under strong sexual selection. Our data reveal that 24% of species carry out a complete post‐juvenile moult, and that this trait has a strong phylogenetic signal. Complete moult is more common in species that inhabit warmer regions and open habitats, show no delayed plumage maturation and have higher levels of sexual dichromatism (indicative of strong sexual selection). Neither the presumed quality of the nest‐grown plumage nor living in regions with high insolation correlates with complete moult. In conclusion, the evolution of complete post‐juvenile moult not only depends on whether birds can perform a complete moult (i.e. suitable environmental conditions) but also on the strength of selection associated with the need of a complete moult. In particular, the necessity to keep the plumage in good condition in challenging environments and the benefits associated with producing adult‐like plumage colours to attract mates or deter rivals seem to play an important role. Moulting strategies in birds have evolved to avoid overlap with, or prepare for, other demanding parts of the annual cycle, such as reproduction or migration. When moulting for the first time after leaving the nest, young birds replace their typically poor‐quality plumage during the post‐juvenile moult. The extent of this moult varies between species from partial to complete. Earlier studies, restricted to Western Palearctic birds, suggest that in most species a complete post‐juvenile moult may not be possible simply because young birds are constrained by not having the same access to resources as adults, unless environmental conditions are favourable. These studies also show that complete post‐juvenile moult is more common in species with poor‐quality nest‐grown plumage. We expanded the spatial and taxonomic scope of previous studies to 1,315 species of passerines from across the world and considered both the role of constraints, plumage quality and other selective pressures favouring a complete post‐juvenile moult. Thus, we test whether complete moult is more prevalent in species where nest‐grown feathers are presumably of poor quality (shorter nestling period), that live in environments that foster quick plumage degradation (open habitats, high insolation and humidity), and where males are under strong sexual selection. Our data reveal that 24% of species carry out a complete post‐juvenile moult, and that this trait has a strong phylogenetic signal. Complete moult is more common in species that inhabit warmer regions and open habitats, show no delayed plumage maturation and have higher levels of sexual dichromatism (indicative of strong sexual selection). Neither the presumed quality of the nest‐grown plumage nor living in regions with high insolation correlates with complete moult. In conclusion, the evolution of complete post‐juvenile moult not only depends on whether birds can perform a complete moult (i.e. suitable environmental conditions) but also on the strength of selection associated with the need of a complete moult. In particular, the necessity to keep the plumage in good condition in challenging environments and the benefits associated with producing adult‐like plumage colours to attract mates or deter rivals seem to play an important role.

Many fields of science-including behavioral ecology-currently experience a heated debate about the extent to which publication bias against null findings results in a misrepresentative scientific literature. Here, we show a case of an extreme mismatch between strong positive support for an effect in the literature and a failure to detect this effect across multiple attempts at replication. For decades, researchers working with birds have individually marked their study species with colored leg bands. For the zebra finch Taeniopygia guttata, a model organism in behavioral ecology, many studies over the past 35 years have reported effects of bands of certain colors on male or female attractiveness and further on behavior, physiology, life history, and fitness. Only eight of 39 publications presented exclusively null findings. Here, we analyze the results of eight experiments in which we quantified the fitness of a total of 730 color-banded individuals from four captive populations (two domesticated and two recently wild derived). This sample size exceeds the combined sample size of all 23 publications that clearly support the "color-band effect" hypothesis. We found that band color explains no variance in either male or female fitness. We also found no heterogeneity in color-band effects, arguing against both context and population specificity. Analysis of unpublished data from three other laboratories strengthens the generality of our null finding. Finally, a meta-analysis of previously published results is indicative of selective reporting and suggests that the effect size approaches zero when sample size is large. We argue that our field-and science in general-would benefit from more effective means to counter confirmation bias and publication bias.

Studies of mate choice typically assume that individuals prefer high quality mates and select them based on condition-dependent indicator traits. In species with bi-parental care, mutual mate choice is expected to result in assortative mating for quality. When assortment is not perfect, the lower quality pair members are expected to compensate by increased parental investment to secure their partner (positive differential allocation). This framework has been assumed to hold for monogamous species like the zebra finch (Taeniopygia guttata), but progress has been hampered by the difficulty to define individual quality. By combining multiple measures of causes (inbreeding, early nutrition) and consequences (ornaments, displays, fitness components) of variation in quality into a single principal component, we here show that quality variation can be quantified successfully. We further show that variation in quality indeed predicts individual pairing success, presumably because it reflects an individual's vigor or ability to invest in reproduction. However, despite high statistical power, we found no evidence for either assortative mating or for positive differential allocation. We suggest that zebra finch ornaments and displays are not sufficiently reliable for the benefits of choosiness to exceed the costs of competition for the putative best partner. To assess the generality of these findings unbiased quantification of signal honesty and preference strength is required, rather than selective reporting of significant results. This article is protected by copyright. All rights reserved

Lay Summary In species where males contribute substantially to parental care, males are expected to be choosy. Males should benefit from selecting a female of high fecundity, but it is unclear to what extent males are able to judge female fecundity before breeding. Here we show that male zebra finches seem to have only limited abilities to identify the better mate when faced with a choice between extremes in terms of female fecundity.

Some species show high rates of reproductive failure, which is puzzling because natural selection works against such failure in every generation. Hatching failure is common in both captive and wild zebra finches (Taeniopygia guttata), yet little is known about its proximate causes. Here we analyze data on reproductive performance (fate of >23,000 eggs) based on up to 14 years of breeding of four captive zebra finch populations. We find that virtually all aspects of reproductive performance are negatively affected by inbreeding (mean r = -0.117), by an early-starting, age-related decline (mean r = -0.132), and by poor early-life nutrition (mean r = - 0.058). However, these effects together explain only about 3% of the variance in infertility, offspring mortality, fecundity and fitness. In contrast, individual repeatability of different fitness components varied between 15% and 50%. As expected, we found relatively low heritability in fitness components (median: 7% of phenotypic, and 29% of individually repeatable variation). Yet, some of the heritable variation in fitness appears to be maintained by antagonistic pleiotropy (negative genetic correlations) between male fitness traits and female and offspring fitness traits. The large amount of unexplained variation suggests a potentially important role of local dominance and epistasis, including the possibility of segregating genetic incompatibilities.

Why do females of socially monogamous species engage in extra-pair copulations? This long-standing question remains a puzzle, because the benefits of female promiscuous behavior often do not seem to outweigh the costs. Genetic constraint models offer an answer by proposing that female promiscuity emerges through selection favoring alleles that are either beneficial for male reproductive success (intersexual pleiotropy hypothesis) or beneficial for female fecundity (intrasexual pleiotropy hypothesis). A previous quantitative genetic study on captive zebra finches, Taeniopygia guttata, reported support for the first, but not for the second hypothesis. Here, we re-examine both hypotheses based on data from lines selected for high and low male courtship rate. In contrast to previous conclusions, our new analyses clearly reject the hypothesis that male and female promiscuity are genetically homologous traits. We find some support for a positive genetic correlation between female promiscuity and fecundity. This study also shows that the behavioral outcome of extra-pair courtships primarily depends on individual-specific female preferences and not on the "attractiveness" of the social mate. In contrast, patterns of paternity are strongly influenced by the social partner and the pair bond, presumably reflecting variation in copulation behavior, fertility, or sperm competitiveness.

It is often claimed that pair bonds preferentially form between individuals that resemble one another. Such assortative mating appears to be widespread throughout the animal kingdom. Yet it is unclear whether the apparent ubiquity of assortative mating arises primarily from mate choice (“like attracts like”), which can be constrained by same-sex competition for mates; from spatial or temporal separation; or from observer, reporting, publication, or search bias. Here, based on a conventional literature search, we find compelling meta-analytical evidence for size-assortative mating in birds (r = 0.178, 95% CI 0.142–0.215, 83 species, 35,591 pairs). However, our analyses reveal that this effect vanishes gradually with increased control of confounding factors. Specifically, the effect size decreased by 42% when we used previously unpublished data from nine long-term field studies, i.e., data free of reporting and publication bias (r = 0.103, 95% CI 0.074–0.132, eight species, 16,611 pairs). Moreover, in those data, assortative mating effectively disappeared when both partners were measured by independent observers or separately in space and time (mean r = 0.018, 95% CI −0.016–0.057). Likewise, we also found no evidence for assortative mating in a direct experimental test for mutual mate choice in captive populations of Zebra finches (r = −0.020, 95% CI −0.148–0.107, 1,414 pairs). These results highlight the importance of unpublished data in generating unbiased meta-analytical conclusions and suggest that the apparent ubiquity of assortative mating reported in the literature is overestimated and may not be driven by mate choice or mating competition for preferred mates., Human mate choice is characterized by assortative mating (‘like attracts like’) and similarity of partners is also often reported for birds. A meta-analysis of published and previously unpublished datasets shows that the reported assortative mating in birds may mostly reflect biases in estimation rather than mate choice., Author summary Research on mate choice in birds has attracted much attention, partly because many birds form monogamous pair bonds like humans do. Human mate choice is characterized by the phenomenon of “like attracts like,” meaning that partners resemble each other in multiple ways (“assortative mating”). Assortative mating is also frequently reported for birds, but it is unclear whether this in turn implies that birds also have preferences for a similar partner. Here, we show that a range of methodological issues may provide a simpler and more accurate explanation for the frequent observation of assortative mating in birds. First, studies that report assortative mating may achieve greater visibility than studies that yield no such finding. Hence, the scientific literature may be biased toward positive results. Second, in field studies, it is logistically impossible to measure all birds accurately and under standardized conditions. Hence, fluctuations in, for instance, environmental conditions may induce a spurious similarity between partners when these are measured together in space or time. After accounting for such methodological issues, we conclude that mate preferences for a similar partner may be less common than previously thought.

Studies on the pattern of geographic variation in bird vocalizations can facilitate the understanding of the evolutionary history of species and species differentiation. The Common Cuckoo (Cuculus canorus) is a non-passerine widely distributed in Eurasia, and its calls are not acquired through learning. Revealing the geographic pattern of Common Cuckoo calls may help our understanding of the relationship between environment, genetic differentiation, and vocal differentiation. In the present study, geographic variation in the calls of the Common Cuckoo was investigated throughout Eurasia for the first time. Calls of different subspecies of the Common Cuckoo were compared, and the correlations between differences in calls, geographic distance, climatic differences, and altitude differences were determined in order to evaluate the influence of subspecies differentiation, isolation by distance and environmental differences on call differentiation. The results showed there to be significant differences in the calls of different subspecies of the Common Cuckoo. Discriminant function analysis was able to correctly identify 81.7 % of individuals to their original subspecies, and 98 % of individuals of subspecies subtelephonus were correctly assigned. Differences in calls both within and between subspecies were found to be significantly correlated with geographic distance, while environmental differences have no important effect. Our study stressed the effect of isolation by distance on geographic variation of non-passerine vocalization, and we infer that the great divergence in calls between different Common Cuckoo subspecies may be a hint of cryptic species.

Many fields of science-including behavioral ecology-currently experience a heated debate about the extent to which publication bias against null findings results in a misrepresentative scientific literature. Here, we show a case of an extreme mismatch between strong positive support for an effect in the literature and a failure to detect this effect across multiple attempts at replication. For decades, researchers working with birds have individually marked their study species with colored leg bands. For the zebra finch Taeniopygia guttata, a model organism in behavioral ecology, many studies over the past 35 years have reported effects of bands of certain colors on male or female attractiveness and further on behavior, physiology, life history, and fitness. Only eight of 39 publications presented exclusively null findings. Here, we analyze the results of eight experiments in which we quantified the fitness of a total of 730 color-banded individuals from four captive populations (two domesticated and two recently wild derived). This sample size exceeds the combined sample size of all 23 publications that clearly support the "color-band effect" hypothesis. We found that band color explains no variance in either male or female fitness. We also found no heterogeneity in color-band effects, arguing against both context and population specificity. Analysis of unpublished data from three other laboratories strengthens the generality of our null finding. Finally, a meta-analysis of previously published results is indicative of selective reporting and suggests that the effect size approaches zero when sample size is large. We argue that our field-and science in general-would benefit from more effective means to counter confirmation bias and publication bias.

Studies of mate choice typically assume that individuals prefer high quality mates and select them based on condition-dependent indicator traits. In species with biparental care, mutual mate choice is expected to result in assortative mating for quality. When assortment is not perfect, the lower quality pair members are expected to compensate by increased parental investment to secure their partner (positive differential allocation). This framework has been assumed to hold for monogamous species like the zebra finch (Taeniopygia guttata), but progress has been hampered by the difficulty to define individual quality. By combining multiple measures of causes (inbreeding, early nutrition) and consequences (ornaments, displays, fitness components) of variation in quality into a single principal component, we here show that quality variation can be quantified successfully. We further show that variation in quality indeed predicts individual pairing success, presumably because it reflects an individual's vigor or ability to invest in reproduction. However, despite high statistical power, we found no evidence for either assortative mating or for positive differential allocation. We suggest that zebra finch ornaments and displays are not sufficiently reliable for the benefits of choosiness to exceed the costs of competition for the putative best partner. To assess the generality of these findings unbiased quantification of signal honesty and preference strength is required, rather than selective reporting of significant results.

Male reproductive success depends on the competitive ability of sperm to fertilize the ova, which should lead to strong selection on sperm characteristics. This raises the question of how heritable variation in sperm traits is maintained. Here we show that in zebra finches (Taeniopygia guttata) nearly half of the variance in sperm morphology is explained by an inversion on the Z chromosome with a 40% allele frequency in the wild. The sperm of males that are heterozygous for the inversion had the longest midpieces and the highest velocity. Furthermore, such males achieved the highest fertility and the highest siring success, both within-pair and extra-pair. Males homozygous for the derived allele show detrimental sperm characteristics and the lowest siring success. Our results suggest heterozygote advantage as the mechanism that maintains the inversion polymorphism and hence variance in sperm design and in fitness. It is unclear how variation in sperm traits is maintained in nature. Here, the authors show that an inversion on the Z chromosome of zebra finches affects sperm morphology and fertilization ability and suggest that overdominance maintains the inversion polymorphism.

In the traditional planning and designing process of water conservancy project, there is a serious gap between the project visualization (“Visualization”) and the project calculation (“Calculation”). Usually, “Calculation” can not be guided by “Visualization”, and “Visualization” can not quickly reflect the result of “Calculation”. In order to resolve the disjoint between “Visualization” and “Calculation”, this article discusses the method of realizing the integration of “Visualization” and “Calculation” in planning and designing water conservancy project by adopting the technologies of Virtual Reality (VR) and Geographic Information System (GIS). The VR technology can provide a realistic, interactive and dynamic three-dimensional scene of the water conservancy project area by computer technologies. By this three-dimensional scene the analysts will get the immersive feeling even if they are not at the scene. GIS can provide the strong function such as spatial data storage and analysis, three-dimensional terrain analysis, and so on, to address its advantage in “Calculation”. So in this article, the three-dimensional virtual environment is built to express the terrain, topography and landscape of the project area, and with this three-dimension environment, the hydroelectric reservoir storage capacity, the submerged area and the volume of excavation and filling can be calculated.

Background Soft song is a low-amplitude song produced by many birds. Recent studies have confirmed that soft song is an aggressive signal. For example, the Brownish-flanked Bush Warblers Cettia fortipes use soft song in male-male conflicts, particularly prior to attacks. Although stable signaling systems require that signals be honest on average, models predict that cheating is an acceptable strategy for some individuals or in some contexts. Methods This study aimed to test the reliability of soft song as an aggressive signal in the brownish-flanked bush warbler. We used mounted specimens accompanied by broadcast songs or soft songs to simulate a male attempting to invade an existing territory. Results We found the mounted specimen that coupled playback of soft songs suffered more and quicker attacks from the territory owner and that the relationship between soft song and subsequent attack in the territory owner was far from perfect. We observed territory owners that both over-signaled (i.e., produced soft song but did not attack) and under-signaled (i.e., attacked without producing soft song). Under-signaling territory owners were relatively more commonly than were over-signaling territory owners, particularly in simulated intrusion that coupled playback of soft song with a mount specimen. Conclusions We discuss the cost of producing soft song and the potential benefit of the unreliable use of soft song and propose a new hypothesis for under-signaling with soft song; i.e., under-signaling territory owners might benefit from taking the initiative in fights.