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1. Association between dietary potassium intake and severe headache or migraine in US adults: a population-based analysis

Author

Lisi Xu, Cong Zhang, Yan Liu, Xiuli Shang, and Daifa Huang

Subjects
potassium intake, migraine, hypertension, ATP-sensitive potassium channels, NHANES, restricted cubic spline, Nutrition. Foods and food supply, TX341-641
Abstract

BackgroundMigraine is a prevalent neurovascular headache disorder. The link between dietary potassium and blood pressure has been established. We sought to delineate the relationship between dietary potassium intake and the prevalence of migraines.MethodsWe conducted a cross-sectional analysis using data from the National Health and Nutrition Examination Survey (NHANES) spanning 1999–2004, comprising 10,254 participants aged ≥20 years. Participants who reported severe headaches or migraine in the self-report questionnaire were identified as migraineurs. A 24-h dietary recall methodology was used to assess dietary potassium intake. Multivariate regression analysis and restricted cubic spline (RCS) modeling were utilized to elucidate the relationship between dietary potassium and migraines.ResultsAmong the 10,254 participants, 20.1% were identified with migraine or severe headaches. The adjusted odds ratio (OR) for migraine occurrence in the Q2 dietary potassium intake (1771–2,476 mg/d) was 0.84 (95% CI: 0.73–0.97, p = 0.021) compared to the lowest quartile (Q1, ≤ 1771 mg/d). The relationship between dietary potassium and migraine exhibited an L-shaped pattern (non-linear, p = 0.016) with an inflection at approximately 1439.3 mg/d. In the subgroup analysis, when compared to Q1, who had the lowest dietary potassium intake, the adjusted OR for Q2 in females, those in the medium-high household income group, and with a Body Mass Index (BMI) ≥ 25 kg/m2 were as follows: (OR, 0.82; 95% CI, 0.69–0.98), (OR, 0.79; 95% CI, 0.66–0.95), and (OR, 0.78; 95% CI, 0.66–0.93), respectively. No significant interaction was observed across groups after adjusting for all possible covariates.ConclusionThe relationship between dietary potassium intake and migraine prevalence among US adults appears to follow an L-shaped curve.

Published
2023
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2. Rab6c is a new target of miR‑218 that can promote the progression of bladder cancer

Author

Daifa Huang, Cheng Du, Long He, Xialu Wang, Peng Chen, Yuhua Cao, and Xiang Pan

Subjects
Cancer Research, China, Cell, Gene Expression, urologic and male genital diseases, Biochemistry, Cell Movement, Cell Line, Tumor, Genetics, medicine, Humans, Neoplasm Invasiveness, Molecular Biology, Cell Proliferation, Bladder cancer, Oncogene, Cell growth, business.industry, Cancer, Cell cycle, medicine.disease, Molecular medicine, Gene Expression Regulation, Neoplastic, MicroRNAs, medicine.anatomical_structure, Oncology, Urinary Bladder Neoplasms, Apoptosis, rab GTP-Binding Proteins, Cancer research, Disease Progression, Molecular Medicine, business
Abstract

Bladder cancer has high morbidity and mortality rates among the male genitourinary system tumor types. MicroRNA‑218 (miR‑218) is associated with the development of a variety of cancer types, including bladder cancer. Rab6c is a member of the Rab family and is involved in drug resistance in MCF7 cells. The aim of the present study was to clarify the relationship between Rab6c and miR‑218 in bladder cancer cell lines. In this study, the expression levels of miR‑218 and Rab6c were evaluated via reverse transcription‑quantitative PCR and western blotting, respectively. The association between Rab6c and miR‑218 was recognized via TargetScan analysis and dual luciferase reporter gene detection. Cell proliferation was analyzed using Cell Counting Kit‑8 and colony formation assays, and the invasive ability was measured via Transwell assays. Rab6c was highly expressed in bladder cancer, while miR‑218 had abnormally low expression in bladder cancer. In addition, there was a mutual regulation between Rab6c and miR‑218 in bladder cancer. It was found that overexpression of Rab6c significantly enhanced the proliferation, colony formation and invasion of T24 and EJ cells. Furthermore, miR‑218 overexpression blocked the promoting effects of Rab6c on the malignant behavior of bladder cancer cells. Thus, Rab6c promotes the proliferation and invasion of bladder cancer cells, while miR‑218 has the opposite effect, which may provide a novel insight for the treatment of bladder cancer.

Published
2020

3. [Cloning and characterization of MTLC, a novel gene in 6q25]

Author

Guangbin, Qiu, Guangrong, Qiu, Zhenming, Xu, Daifa, Huang, Liguo, Gong, Chunyi, Li, Xinghe, Sun, and Kailai, Sun

Subjects
DNA, Complementary, Base Sequence, Sequence Homology, Amino Acid, Recombinant Fusion Proteins, Green Fluorescent Proteins, Molecular Sequence Data, Gene Expression, Nuclear Proteins, Sequence Analysis, DNA, Blotting, Northern, Luminescent Proteins, Microscopy, Fluorescence, Tumor Cells, Cultured, Humans, Chromosomes, Human, Pair 6, Amino Acid Sequence, Cloning, Molecular, Laryngeal Neoplasms, Sequence Alignment
Abstract

To identify and characterize laryngeal cancer related novel genes located on chromosome 6q25.Electric hybridization was performed in human genome database using EST (expression sequence tag) as probe. Novel genes were deduced by software from positive DNA clones and their cDNAs were amplified by RT-PCR using primers designed according to the sequence of the putative genes.A novel gene was cloned successfully. The full length of this gene was about 21 kb. It contained two exons and produced a 1006 bp transcript coding a protein with 235 amino acid residues. It's 5'flanking sequence contained two binding sites of oncoprotein c-Myc, thus it was named MTLC (c-Myc target from laryngeal cancer cells). Homologous assay showed that MTLC exhibited little overall homology to known human proteins but it exhibited good overall homology to mouse MT-MC1 protein with an identity of 78%. The primary structure of MTLC protein contained a nuclear location signal motif, but it did not have other conserved domains. The results of subcellular location experiment showed that MTLC expressed in nuclei of human hepatocellular carcinoma cell line Bel7402 cells, while a wide distribution of MTLC in various tissues was demonstrated by Northern blotting.MTLC may play an important role as a target gene of c-Myc and as a transcription factor in keeping the normal physiological process of cells.

Published
2003

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