23 results on '"Dai, Zhu-Bo"'
Search Results
2. Cloning and functional analysis of two sterol-C24-methyltransferase 1 (SMT1) genes from Paris polyphylla
- Author
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Guan, Hong-Yu, primary, Su, Ping, additional, Zhao, Yu-Jun, additional, Zhang, Xia-Nan, additional, Dai, Zhu-Bo, additional, Guo, Juan, additional, Tong, Yu-Ru, additional, Liu, Yu-Jia, additional, Hu, Tian-Yuan, additional, Yin, Yan, additional, Gao, Lin-Hui, additional, Gao, Wei, additional, and Huang, Lu-Qi, additional
- Published
- 2017
- Full Text
- View/download PDF
3. Cloning and functional analysis of two sterol-C24-methyltransferase 1 (SMT1) genes from Paris polyphylla.
- Author
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Guan, Hong-Yu, Su, Ping, Zhao, Yu-Jun, Zhang, Xia-Nan, Dai, Zhu-Bo, Guo, Juan, Tong, Yu-Ru, Liu, Yu-Jia, Hu, Tian-Yuan, Yin, Yan, Gao, Lin-Hui, Gao, Wei, and Huang, Lu-Qi
- Subjects
ADENOSYLMETHIONINE ,DNA ,GENE expression ,GENETIC techniques ,MEDICINAL plants ,MOLECULAR structure ,POLYMERASE chain reaction ,RESEARCH funding ,TRANSFERASES ,REVERSE transcriptase polymerase chain reaction ,PHYTOSTEROLS ,SEQUENCE analysis ,IN vitro studies - Abstract
The biosynthetic pathways of phytosterols and steroidal saponins are located in two adjacent branches which share cycloartenol as substrate. The rate-limiting enzyme S-adenosyl-L-methionine-sterol-C24-methyltransferase 1 (SMT1) facilitates the metabolic flux toward phytosterols. It catalyzes the methylation of the cycloartenol in the side chain of the C24-alkyl group, to generate 24(28)-methylene cycloartenol. In this study, we obtained two full-length sequences of SMT1 genes from Pari polyphylla, designated PpSMT1-1 and PpSMT1-2. The full-length cDNA of PpSMT1-1 was 1369 bp long with an open reading frame (ORF) of 1038 bp, while the PpSMT1-2 had a length of 1222 bp, with a 1005 bp ORF. Bioinformatics analysis confirmed that the two cloned SMTs belong to the SMT1 family. The predicted function was further validated by performing in vitro enzymatic reactions, and the results showed that PpSMT1-1 encodes a cycloartenol-C24-methyltransferase, which catalyzes the conversion of cycloartenol to 24-methylene cycloartenol, whereas PpSMT1-2 lacked this catalytic activity. The tissue expression patterns of the two SMTs revealed differential expression in different organs of Paris polyphylla plants of different developmental stage and age. These results lay the foundation for detailed genetic studies of the biosynthetic pathways of steroid compounds, which constitute the main class of active substances found in P. polyphylla. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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4. Molecular Pharmacognosy: A new Borderline Discipline
- Author
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Huang, Lu-Qi, primary, Yuan, Yuan, additional, Cui, Guang-Hong, additional, Dai, Zhu-Bo, additional, and Xiao, Pei-Gen, additional
- Published
- 2009
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5. Research progress of synthetic biology for tanshinones.
- Author
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GAO Wei, HU Tian-yuan, GUO Juan, LV Dong-mei, DAI Zhu-bo, ZHOU Yong-jin, and HUANG Lu-qi
- Published
- 2015
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- View/download PDF
6. Status and future of natural resource for Chinese materia medica.
- Author
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MA Xiao-jing, GUO Juan, TANG Jin-fu, MA Xiao-hui, MA Ying, DAI Zhu-bo, GUO Lan-ping, and HUANG Lu-qi
- Published
- 2015
- Full Text
- View/download PDF
7. Construction of Saccharomyces cerevisiae cell factories for lycopene production.
- Author
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SHI Ming-yu, LIU Yi, WANG Dong, LU Fu-ping, Huang Lu-qi, DAI Zhu-bo, and ZHANG Xue-li
- Published
- 2014
- Full Text
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8. Optimization of synthetic pathway and fermentation process of yeast cell factories for production of oleanoic acid.
- Author
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WANG Dong la, WANG Bei-bei, LIU Yi, SHI Ming-yu, XIAO Dong-guang, HUANG Lu-qi, DAI Zhu-bo, and ZHANG Xue-li
- Published
- 2014
- Full Text
- View/download PDF
9. [Heterologous biomimetic synthesis of active ingredients in traditional Chinese medicine:a new mode for protection and development of traditional Chinese medicine resources].
- Author
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Wang J and Dai ZB
- Subjects
- Animals, Medicine, Chinese Traditional, Biomimetics, Drugs, Chinese Herbal, Plants, Medicinal, Alkaloids
- Abstract
Heterologous biomimetic synthesis of the active ingredients of traditional Chinese medicine(TCM) is a new mode of resource acquisition and has shown great potential in the protection and development of TCM resources. According to synthetic biology and by constructing biomimetic microbial cells and imitating the synthesis of active ingredients in medicinal plants and animals, the key enzymes obtained from medicinal plants and animals are scientifically designed and systematically reconstructed and optimized to realize the heterologous synthesis of the active ingredients in microorganisms. This method ensures an efficient and green acquisition of target products, and also achieves large-scale industrial production, which is conducive to the production of scarce TCM resources. Additiona-lly, the method playes a role in agricultural industrialization, and provides a new option for promoting the green and sustainable deve-lopment of TCM resources. This review systematically summarized the important progress in the heterologous biomimetic synthesis of TCM active ingredients from three research areas: biosynthesis of terpenoids, flavonoids, phenylpropanoids, alkaloids and other active ingredients, key points and difficulties in heterologous biomimetic synthesis, and biomimetic cells with complex TCM ingredients. This study facilitated the application of new generation of biotechnology and theory to the development of TCM.
- Published
- 2023
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10. [Construction of cell factories for production of patchoulol in Saccharomyces cerevisiae].
- Author
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Guo S, Wang D, Yang TT, Li WH, Li RS, Zhang GW, Zhang XL, and Dai ZB
- Subjects
- Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae metabolism, Sesquiterpenes metabolism, Pogostemon, Oils, Volatile metabolism
- Abstract
Patchoulol is an important sesquiterpenoid in the volatile oil of Pogostemon cablin, and is also considered to be the main contributing component to the pharmacological efficacy and fragrance of P. cablin oil, which has antibacterial, antitumor, antioxidant, and other biological activities. Currently, patchoulol and its essential oil blends are in high demand worldwide, but the traditional plant extraction method has many problems such as wasting land and polluting the environment. Therefore, there is an urgent need for a new method to produce patchoulol efficiently and at low cost. To broaden the production method of patchouli and achieve the heterologous production of patchoulol in Saccharomyces cerevisiae, the patchoulol synthase(PS) gene from P. cablin was codon optimized and placed under the inducible strong promoter GAL1 to transfer into the yeast platform strain YTT-T5, thereby obtaining strain PS00 with the production of(4.0±0.3) mg·L~(-1) patchoulol. To improve the conversion rate, this study used protein fusion method to fuse SmFPS gene from Salvia miltiorrhiza with PS gene, leading to increase the yield of patchoulol to(100.9±7.4) mg·L~(-1) by 25-folds. By further optimizing the copy number of the fusion gene, the yield of patchoulol was increased by 90% to(191.1±32.7) mg·L~(-1). By optimizing the fermentation process, the strain was able to achieve a patchouli yield of 2.1 g·L~(-1) in a high-density fermentation system, which was the highest yield so far. This study provides an important basis for the green production of patchoulol.
- Published
- 2023
- Full Text
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11. [Construction of cell factories for high production of ginsenoside Rh_2 in Saccharomyces cerevisiae].
- Author
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Shi YS, Wang D, Li RS, Zhang XL, and Dai ZB
- Subjects
- Fermentation, Humans, Saccharomyces cerevisiae genetics, Uridine Diphosphate Glucose, Ginsenosides, Panax genetics, Panax notoginseng
- Abstract
Ginsenoside Rh_2 is a rare active ingredient in precious Chinese medicinal materials such as Ginseng Radix et Rhizoma, Notoginseng Radix et Rhizoma, and Panacis Quinquefolii Radix. It has important pharmacological activities such as anti-cancer and improving human immunity. However, due to the extremely low content of ginsenoside Rh_2 in the source plants, the traditional way of obtaining it has limitations. This study intended to apply synthetic biological technology to develop a cell factory of Saccharomyces cerevisiae to produce Rh_2 by low-cost fermentation. First, we used the high protopanaxadiol(PPD)-yielding strain LPTA as the chassis strain, and inserted the Panax notoginseng enzyme gene Pn1-31, together with yeast UDP-glucose supply module genes[phosphoglucose mutase 1(PGM1), α-phosphoglucose mutase(PGM2), and uridine diphosphate glucose pyrophosphorylase(UGP1)], into the EGH1 locus of yeast chromosome. The engineered strain LPTA-RH2 produced 17.10 mg·g~(-1) ginsenoside Rh_2. This strain had low yield of Rh_2 while accumulated much precursor PPD, which severely restricted the application of this strain. In order to further improve the production of ginsenoside Rh_2, we strengthened the UDP glucose supply module and ginsenoside Rh_2 synthesis module by engineered strain LPTA-RH2-T. The shaking flask yield of ginsenoside Rh_2 was increased to 36.26 mg·g~(-1), which accounted for 3.63% of the dry weight of yeast cells. Compared with those of the original strain LPTA-RH2, the final production and the conversion efficiency of Rh_2 increased by 112.11% and 65.14%, respectively. This study provides an important basis for further obtaining the industrial-grade cell factory for the production of ginsenoside Rh_2.
- Published
- 2022
- Full Text
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12. [Dynamic control of ERG20 expression to improve production of monoterpenes by engineering Saccharomyces cerevisiae].
- Author
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Li RS, Wang D, Shi YS, Xu LP, Zhang XL, Wang K, and Dai ZB
- Subjects
- Fermentation, Geranyltranstransferase genetics, Monoterpenes metabolism, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae metabolism, Saccharomyces cerevisiae Proteins genetics, Saccharomyces cerevisiae Proteins metabolism
- Abstract
Monoterpenes are widely used in cosmetics, food, medicine, agriculture and other fields. With the development of synthetic biology, it is considered as a potential way to create microbial cell factories to produce monoterpenes. Engineering Saccharomyces cerevisiae to produce monoterpenes has been a research hotspot in synthetic biology. In S. cerevisiae, the production of geranyl pyrophosphate(GPP) and farnesyl pyrophosphate(FPP) is catalyzed by a bifunctional enzyme farnesyl pyrophosphate synthetase(encoded by ERG20 gene) which is inclined to synthesize FPP essential for yeast growth. Therefore, reasonable control of FPP synthesis is the basis for efficient monoterpene synthesis in yeast cell factories. In order to achieve dynamic control from GPP to FPP biosynthesis in S. cerevisiae, we obtained a novel chassis strain HP001-pERG1-ERG20 by replacing the ERG20 promoter of the chassis strain HP001 with the promoter of cyclosqualene cyclase(ERG1) gene. Further, we reconstructed the metabolic pathway by using GPP and neryl diphosphate(NPP), cis-GPP as substrates in HP001-pERG1-ERG20. The yield of GPP-derived linalool increased by 42.5% to 7.6 mg·L~(-1), and that of NPP-derived nerol increased by 1 436.4% to 8.3 mg·L~(-1). This study provides a basis for the production of monoterpenes by microbial fermentation.
- Published
- 2022
- Full Text
- View/download PDF
13. [Optimization of UDP-glucose supply module and production of ginsenoside F 1 in Saccharomyces cerevisiae].
- Author
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Wang JH, Wang D, Li WX, Huang Y, Dai ZB, and Zhang XL
- Subjects
- Glucose, Uridine Diphosphate Glucose, Ginsenosides metabolism, Panax, Saccharomyces cerevisiae metabolism
- Abstract
Ginsenoside F1 is a rare ginsenoside in medicinal plants such as Panax ginseng,P. notogingseng and P. quinquefolius. It has strong pharmacological activities of anti-tumor,anti-oxidation and anti-aging. In order to directly produce ginsenoside F1 by using inexpensive raw materials such as glucose,we integrated the codon-optimized P.ginseng dammarenediol-Ⅱ synthase(Syn Pg DDS),P.ginseng protopanaxadiol synthase(Syn Pg PPDS),P. ginseng protopanaxatriol synthase(Syn Pg PPTS) genes and Arabidopsis thaliana cytochrome P450 reductase(At CPR1) gene into triterpene chassis strain BY-T3. The transformant BY-PPT can produce protopanaxatriol. Then we integrated the Sacchromyces cerevisiae phosphoglucomutase 1(PGM1),phosphoglucomutase 2(PGM2) and UDP-glucose pyrophosphorylase 1(UGP1) genes into chassis strain BY-PPT. The UDP-glucose supply module increased UDP-glucose production by 8. 65 times and eventually reached to 44. 30 mg·L-1 while confirmed in the transformant BY-PPT-GM. Next,we integrated the UDPglucosyltransferase Pg3-29 gene which can catalyze protopanaxatriol to produce ginsenoside F1 into chassis strain BY-PPT-GM. The transformant BY-F1 produced a small amount of ginsenoside F1 which was measured as 0. 5 mg·L-1. After the fermentation process was optimized,the titer of ginsenoside F1 could be increased by 900 times to 450. 5 mg·L-1. The high-efficiency UDP-glucose supply module in this study can provide reference for the construction of cell factories for production of saponin,and provide an important basis for further obtaining high-yield ginsenoside yeast cells.
- Published
- 2019
- Full Text
- View/download PDF
14. [Biosynthesis analysis of hederagenin pathway and its construction in yeast cells].
- Author
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Ma XL, Li WX, Wang D, Lu FP, Dai ZB, and Zhang XL
- Subjects
- Biosynthetic Pathways, Cell Count, Oleanolic Acid metabolism, Saccharomyces cerevisiae, Oleanolic Acid analogs & derivatives
- Abstract
Hederagenin is an effective constituent of many medical plants, such as Clematidis Radix, and has a wide range of applications in anti-tumor, anti-inflammatory, antidepressant, hepatoprotective antibacterial, et al. In order to obtain the efficient production of yeast cells for hederagenin,we successfully cloned and screened out a P450 gene MdMA02 from Malus×domestica which can catalyze oleanolic acid C-23 oxidation with our developed plug and play platform. Its amino acid homology is only 32% as compared to characterized CYP72A68v2. By transforming MdMA02 to the oleanolic acid-producing strain BY-OA, a hederagenin-producing strain was constructed and hederagenin's titer could achieve 101 mg·L⁻¹ using high cell density fermentation, which was 337 times higher than in shake flasks culturing. This study provides a basis for further research on promoting the creation of oleanane-type pentacyclic triterpenoids biosynthetic pathway analysis and relative cell factories construction., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Chinese Pharmaceutical Association.)
- Published
- 2018
- Full Text
- View/download PDF
15. [Study of heterologous efficient synthesis of cucurbitadienol].
- Author
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Li SL, Wang D, Liu Y, Lin TT, Tang JL, Hua EB, Zhang XL, Dai ZB, and Huang LQ
- Subjects
- Fermentation, Gas Chromatography-Mass Spectrometry, Industrial Microbiology, Microorganisms, Genetically-Modified, Saccharomyces cerevisiae metabolism, Cucurbitaceae enzymology, Triterpenes metabolism
- Abstract
Cucurbitadienol has anti-inflammation, anti-cancer activities, and acts as a precursor of traditional Chinese medicine active ingredients mogroside and cucurbitacine. For construction of a Sacchromyces cerevisiae cell factory for production of cucurbitadienol, we firstly cloned a cucurbitadienol synthase (CBS) gene from Siraitia grosvenorii. Then, through heterologous expression of CBS in the triterpenoid chassis strain WD-2091, the engineered strain could produced 27.44 mg•L ⁻¹ cucurbitadienol, which was determined by GC-MS. Further regulation of CBS expression led to cucurbitadienol's titer increasing by 202.07% and reaching 82.89 mg•L ⁻¹ in the shake flask fermentation and 1 724.10 mg•L ⁻¹ in the high cell density fermentation. Our research promotes the cucurbitane-type tetracyclic triterpenoids synthesis pathway analysis progress and provides the basis for further obtaining cell factories for production of cucurbitadienol tetracyclic triterpenoids., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Chinese Pharmaceutical Association.)
- Published
- 2017
- Full Text
- View/download PDF
16. [Construction of cell factories for high production of nerolidol in Saccharomyces cerevisiae].
- Author
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Zhang LL, Ma XL, Wang D, Yu P, Huang LQ, Zhang XL, and Dai ZB
- Subjects
- Fermentation, Industrial Microbiology, Saccharomyces cerevisiae metabolism, Sesquiterpenes metabolism
- Abstract
Nerolidol is an important constituent of terpenoid essential oil and has excellent anti-tumor, anti-bacterial, and anti-oxidative properties. For realizing heterogenous production of nerolidol, our research firstly integrated the codon-optimized Actinidia sinensis nerolidol synthase gene (NES) into the terpenoid chassis strain FPP-001, and obtained NES-001 that could produce 2.71 mg•L⁻¹ nerolidol. Then, the N-terminal of the NES was fused with FPS by linker peptide GGGS. With this strategy, nerolidol production improved by 59.80-fold, reaching 162.07 mg•L⁻¹. Finally, by introduction of auxotrophic marker TRP1 in NES-002, the resulting strain NES-003 could produce 1 711.53 mg•L⁻¹ by high cell density fermentation method. This study provides the basis for the fermentative production of nerolidol and other sesquiterpenoids., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Chinese Pharmaceutical Association.)
- Published
- 2017
- Full Text
- View/download PDF
17. [Construction of cell factories for production of lupeol in Saccharomyces cerevisiae].
- Author
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Lin TT, Wang D, Dai ZB, Zhang XL, and Huang LQ
- Subjects
- Biosynthetic Pathways, Metabolic Engineering, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae Proteins genetics, Saccharomyces cerevisiae Proteins metabolism, Triterpenes metabolism, Betulinic Acid, Pentacyclic Triterpenes biosynthesis, Saccharomyces cerevisiae metabolism
- Abstract
Lupane-type triterpenoids, such as betulinic acid, are derived from lupeol and have excellent properties in anti-HIV, anti-cancer activities and so on. For realizing heterogenous production of lupane-type triterpenoids, our research firstly integrated all the seven genes in the MVA pathway in Saccharomyces cerevisiae to increase the supply of squalene (triterpenoids universal precursor) in a single step using the DNA assembler method. Next, cell factories for production of lupeol was constructed by integrating Arabidopsis thaliana lupeol synthetic gene (AtLUP) into chromosome of triterpenoid chassis strain. Results showed that the MVA pathway, about 20 kb nucleotide length, could be assembled in one-pot process and the doubled MVA pathway could significantly improve squalene by 500-fold, reaching 354.00 mg•L⁻¹. NK2-LUP was obtained by introducing AtLUP gene on chromosome, and could produce 8.23 mg•L⁻¹ lupeol. This study supports the possibility of large-scale biosynthetic pathway assembly in S.cerevisiae and lays the foundation of obtaining cell factories for production of lupan-type triterpenoids at the same time., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Chinese Pharmaceutical Association.)
- Published
- 2016
- Full Text
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18. Research progress of synthetic biology for tanshinones.
- Author
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Gao W, Hu TY, Guo J, Lv DM, Dai ZB, Zhou YJ, and Huang LQ
- Subjects
- Medicine, Chinese Traditional, Salvia miltiorrhiza metabolism, Synthetic Biology, Abietanes biosynthesis
- Abstract
Synthetic biology research methods which design and build a new artificial biological systems (medicinal plants or microorganisms system) with specific physiological functions through clarifying and simulating the basic law of the biosynthesis of active components of traditional Chinese medicine, is considered to be a potential method to produce an abundant resources of bioactive components. Tanshinones is a kind of diterpene quinone compounds with important pharmacological activities from traditional Chinese medicine Salvia miltiorrhiza. This article systematically introduced the research progress of the synthetic biology of S. miltiorrhiza, in order to provide references for studies on other terpenoid bioactive components of traditional Chinese medicines, and give new research strategies for the sustainable development of traditional Chinese medicine resources.
- Published
- 2015
19. [Status and future of natural resource for Chinese materia medica].
- Author
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Ma XJ, Guo J, Tang JF, Ma XH, Ma Y, Dai ZB, Guo LP, and Huang LQ
- Subjects
- Chemistry, Pharmaceutical, Drugs, Chinese Herbal pharmacology, Humans, Materia Medica pharmacology, Conservation of Natural Resources, Drugs, Chinese Herbal chemistry, Materia Medica chemistry, Medicine, Chinese Traditional trends
- Abstract
For thousands of years, the natural resource for Chinese materiamedica has been the foundation of the traditional Chinese medicine industry, which provides abundant medicine for human. In recent years, increasing demands and irrational exploitation led to a lot of problems such as rapid decrease of traditional Chinese herbs reserves, low quality of medicine and dismishing traditional cultures. These restricted the development of the traditional Chinese medicine. To solve these problems, scientists have done much work on investigating traditional Chinese medicine resources, exploring the metabolic pathway of bioactive ingredients, cultivating new varieties, and carrying out synthetic biology. These studies provided a theoretical basis for sustainable utilizationand future developmentof traditional Chinese medicine resources.
- Published
- 2015
20. [Construction of Saccharomyces cerevisiae cell factories for lycopene production].
- Author
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Shi MY, Liu Yi, Wang D, Lu FP, Huang LQ, Dai ZB, and Zhang XL
- Subjects
- Bacterial Proteins genetics, Bacterial Proteins metabolism, Biosynthetic Pathways, Genes, Synthetic, Genetic Engineering, Lycopene, Pantoea enzymology, Pantoea genetics, Carotenoids biosynthesis, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae metabolism
- Abstract
For microbial production of lycopene, the lycopene synthetic genes from Pantoea agglomerans were integrated into Saccharomyces cerevisiae strain BY4742, to obtain strain ZD-L-000 for production of 0.17 mg · L(-1) lycopene. Improving supplies of isoprenoid precursors was then investigated for increasing lycopene production. Four key genes were chosen to be overexpressed, inclu- ding truncated 3-hydroxy-3-methylglutaryl-CoA reductase gene (tHMG1), which is the major rate-limiting enzyme in the mevalonate (MVA) pathway, a mutated global regulatory factor gene (upc2.1), a fusion gene of FPP synthase (ERG20) and endogenous GGPP synthase (BTS1), which is a key enzyme in the diterpenoid synthetic pathway, and GGPP synthase gene (SaGGPS) from Sulfolobus acidocaldarius. Over-expression of upc2.1 could not improve lycopene production, while over-expression of tHMGI , BTS1-ERG20 and SaGGPS genes led to 2-, 16. 9- and20. 5-fold increase of lycopene production, respectively. In addition, three effective genes, tHMG1, BTS1-ERG20 and SaGGPS, were integrated into rDNA sites of ZD-L-000, resulting in strain ZD-L-201 for production of 13.23 mg · L(-1) lycopene, which was 77-fold higher than that of the parent strain. Finally, two-phase extractive fermentation was performed. The titer of lycopene increased 10-fold to 135.21 mg · L(-1). The engineered yeast strains obtained in this work provided the basis for fermentative production of lycopene.
- Published
- 2014
21. [Optimization of synthetic pathway and fermentation process of yeast cell factories for production of oleanoic acid].
- Author
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Wang D, Wang BB, Liu Y, Shi MY, Xiao DG, Huang LQ, Dai ZB, and Zhang XL
- Subjects
- Biomass, Dose-Response Relationship, Drug, Glucose pharmacology, Saccharomyces cerevisiae drug effects, Biotechnology methods, Fermentation, Oleanolic Acid biosynthesis, Saccharomyces cerevisiae cytology, Saccharomyces cerevisiae metabolism
- Abstract
Objective: To optimize the synthetic pathway and fermentation process of yeast cell factories for production of oleanoic acid., Method: Using the DNA assembler method, one copy of Glycyrrhiza glabra beta-amyrin synthase (GgbAS), Medicago truncatula oleanolic acid synthase (MtOAS) and Arabidopsis thaliana cytochrome P450 reductase 1 (AtCPR1) genes were introduced into Saccharomyces cerevisiae strain BY-OA, resulting in strain BY-20A. YPD medium with different glucose concentration were then used to cultivate strain BY-2OA., Result: Increasing gene copies of GgbAS, MtOAS and AtCPR1 resulted in increased beta-amyrin and oleanolic acid production. The strain BY-2OA produced 136.5 mg x L(-1) beta-amyrin and 92.5 mg x L(-1) oleanolic acid, which were 54% and 30% higher than the parent strain BY-OA. Finally, the titer of oleanolic acid increased to 165.7 mg x L(-1) when cultivated in YPD medium with 40 mg x L(-1) glucose., Conclusion: Production of oleanoic acid increased significantly in the yeast strain BY-2OA, which can provide the basis for creating an alternative way for production of oleanoic acid in place of extraction from plant sources.
- Published
- 2014
22. [Cloning and characterization of cDNA encoding Psammosilene tunicoides squalene synthase].
- Author
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Dai ZB, Qian ZG, Hu YQ, and Huang LQ
- Subjects
- Caryophyllaceae genetics, Cloning, Molecular, DNA, Complementary genetics, Endangered Species, Escherichia coli genetics, Escherichia coli metabolism, Farnesyl-Diphosphate Farnesyltransferase metabolism, Gas Chromatography-Mass Spectrometry, Open Reading Frames, Phylogeny, Plant Proteins metabolism, Plants, Medicinal chemistry, Plants, Medicinal genetics, Plasmids, Polymerase Chain Reaction, Recombinant Proteins metabolism, Sequence Homology, Amino Acid, Transformation, Genetic, Caryophyllaceae enzymology, Farnesyl-Diphosphate Farnesyltransferase genetics, Plant Proteins genetics
- Abstract
The total triterpene saponins of Psammosilene tunicoides have significant pharmacologic activity. Psammosilene tunicoides squalene synthase (PSS) is a gateway enzyme to regulate the biosynthesis of total triterpene saponins extracted from the root of Psammosilene tunicoides which is an endangered species. In this paper, cDNA encoding of PSS was cloned by the degenerate primer PCR and rapid-amplification of cDNA ends (RACE). The full-length of cDNA of PSS is 1663 bp, with an open reading frame (ORF) of 1 245 bp, encoding 414 amino acid polypeptide (calculated molecular mass, 47.69 kDa), 5'UTR (untranslated region) and 3'UTR are 260 bp and 158 bp, respectively. The deduced amino acid sequence of PSS has higher homology with the known squalene synthases of several species such as Panax notoginseng (83%), Panax ginseng (82%) and Glycyrrhiza glabra (82%) than that with Schizosacharomyces pombe (35%), Candida albicans (39%) and Homo sapiens (47%). The characterization of PSS was done by a series of methods, such as prokaryotic expression, the activity of enzyme in vitro, capillary gas chromatography (GC) and capillary gas chromatography mass spectrometry (GC-MS). The results showed that the cell-free extract of E. coli transformed with the recombinant plasmid can effectively convert farnesyl diphosphate into squalene in vitro. GenBank accession number is EF585250. Our research provided important base for the study of Psammosilene tunicoides secondary metabolism and metabolic engineering.
- Published
- 2008
23. [Ribosomal DNA ITS sequence of analysis of Psammosilene tunicoides from different populations].
- Author
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Liu WZ, Dai ZB, and Qian ZG
- Subjects
- Base Sequence, Caryophyllaceae classification, Caryophyllaceae growth & development, China, DNA, Plant chemistry, DNA, Plant genetics, DNA, Plant isolation & purification, Geography, Molecular Sequence Data, Phylogeny, Plants, Medicinal growth & development, Polymerase Chain Reaction, Sequence Analysis, DNA, Caryophyllaceae genetics, DNA, Ribosomal Spacer genetics, Plants, Medicinal genetics, RNA, Ribosomal, 5.8S genetics
- Abstract
Objective: To analyze the ribosomal ITS sequence variation of Psammosilene tuncolides W. C. Wu et C. Y. Wu from different populations, for identifying different local populations., Methods: A pair of primers of 18SP1 and 26SP2 with PCR technique had been applied to study the ITS sequences., Results: The sequences of ITS1, ITS2 and 5.8S are 225-229 bp, 166-170 bp and 261-264 bp. Among 12 local populations, the sequence of Kunming, Lijiang, Gejiu, Heqing of Yunnan and Yanyuan of Sichuan showed no variation, there were 1-4 variable sites (including 5.8S coding region) in pairwise comparison of the other 7 local populations, including Xuanwei, Huize, Zhongdian, Baoshan of Yunnan, Muli of Sichuan and Linzhi of Tibet., Conclusion: Comparative analysis shows that the ITS sequences of different local populations in the middle of Yunnan, southwest of Sichuan and west, northwest of Yunnan, southeast of Tibet, southwest of Sichuan have different fingerprint character, so the ITS sequences can be used to identify different local populations. The variation of ITS sequence of Psammosilene tunicoides is related to its geographical distribution.
- Published
- 2008
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