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Research and International Conference AlphaS1-Casein gene is a gene that controls the quality of the milk protein. Alleles of AlphaS1-Casein gene can be grouped into four levels of expression for the quality of the protein in milk, which is high (A, B1, B2, B3, B4, C, H, L and M), intermediate (E and I), weak (D, F and G) and null alleles (O1, O2, and N). The purpose of this study was to characterize AlphaS1-Casein gene in Indonesian Local goats by knowing allele frequencies and heterozygosity values by using PCR-RFLP techniques using XmnI restriction enzyme. This study found 2 alleles, ie. A allele with frequencies of 0.94, and B allele with 0.06. These two alleles were included in the group of high expression casein (high protein quality). In the local goat population, Ho values obtained was 0.223 and He was 0.376. The results showed that the genetic diversity of AlphaS1-Casein gene was low.

Growth Hormone (GH) is a hormone produced by cells in the anterior lobe of the pituitarysomatrotop and formation process under the control of GH gene. One important function of thishormone is to help the process of tissue formation and metabolism of fat to meat forming. Thepurpose of this study was to determine the genetic diversity of genes GH Kacang goat in subpopulations of Kota Gorontalo and Regency of Bone Bolango. Blood samples were used for DNAextraction process in Centre of Biotechnology Laboratory University of Hasanuddin is 41 samples ofKacang goats with 21 samples from Kota Gorontalo city and 20 samples from Regency of BoneBolango. Genomic DNA was extracted using a kit DNA extraction Genjet Genomic DNA Extraction(Thermo Scientific) following standard protocol phenol-chloroform, amplified by the technique ofPolymerase Chain Reaction (PCR), and genotyping was done by Polymerase Chain ReactionRestrictionFragment Length Polymorphism (PCR-RFLP) using the restriction enzyme Hae III. Datawere analyzed descriptively by calculating the frequency of genotype, allele frequency, and degreeof heterozygosity. The results showed GH genotype frequencies for the genotypes AA and AB were2.45 and 97.5% respectively and the frequency of alleles A and B were 51.2 and 48.7% per centrespectively and the observed heterozygosity (Ho) and expected heterozygosity (He) were 0.97 and0.50 respectively. Based on the sub-population genotype frequencies obtained GH gene of Kacanggoat from Kota Gorontalo is 95.25 % for AA and 4.76% for AB, the frequency of allele A and B was52.3% 47.6%, observated heterozygosity (Ho) 0.95 and expected heterozygosity (He ) 0.51. GH genegenotype frequencies in Kacang goat from Regency of Bone Bolango is AB 100%, the frequency ofallele A and allele B 0.5 0.5, observation heterozygosity (Ho) of 1.00 and expectation heterozygosity(He) 0.51. Based on the results concluded GH gene Kacang goat from Kota Gorontalo and Regency ofBone Bolango is polymorphic so that it can be used as the basis for the implementation of theselection.Key Words: Genetic Diversity, Growth Hormone, Kacang Goat