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9 results on '"Daby, Camden L."'

2. Refined Homology-Directed Repair Methodological Approaches of Donorguide, a Chimeric RNA: DNA tracrRNA.

Author

Morales Gomez, Adriana, Rai Thulung, Lucy, Daby, Camden L., Savage, Kaila S., Clark, Karl J., and Ekker, Stephen C.

Subjects
RNA, DNA, BRACHYDANIO, DNA insertion elements
Abstract

The use of single-stranded oligodeoxynucleotide (ssODN) along with CRISPR–Cas9-mediated double-strand breaks (DSB) is one of the most commonly deployed methods for introducing genetic alterations, but this approach has notable limitations. Recognizing this, we have developed a protocol article that provides a step-by-step process of donorguide, a covalent fusion of trans-activating CRISPR RNA (tracrRNA) and ssODN. Donorguide has the potential to enhance the introduction of specific genetic alterations (insertion, deletion, and substitution) at a DSB, improving homology-directed repair methods from zebrafish in vivo to human cells in vitro. We also explored and discuss the impact of increasing the length of donorguide homology arms in zebrafish. [ABSTRACT FROM AUTHOR]

Published
2024
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4. Chimeric RNA:DNA Donorguide Improves HDR in vitro and in vivo

Author

Simone, Brandon W., primary, Lee, Han B., additional, Daby, Camden L., additional, Restrepo-Castillo, Santiago, additional, Martínez-Gálvez, Gabriel, additional, Ata, Hirotaka, additional, Gendron, William A.C., additional, Clark, Karl J., additional, and Ekker, Stephen C., additional

Published
2021
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5. Building the vertebrate codex using the gene breaking protein trap library

Author

Ichino, Noriko, primary, Serres, MaKayla R, additional, Urban, Rhianna M, additional, Urban, Mark D, additional, Treichel, Anthony J, additional, Schaefbauer, Kyle J, additional, Tallant, Lauren E, additional, Varshney, Gaurav K, additional, Skuster, Kimberly J, additional, McNulty, Melissa S, additional, Daby, Camden L, additional, Wang, Ying, additional, Liao, Hsin-kai, additional, El-Rass, Suzan, additional, Ding, Yonghe, additional, Liu, Weibin, additional, Anderson, Jennifer L, additional, Wishman, Mark D, additional, Sabharwal, Ankit, additional, Schimmenti, Lisa A, additional, Sivasubbu, Sridhar, additional, Balciunas, Darius, additional, Hammerschmidt, Matthias, additional, Farber, Steven Arthur, additional, Wen, Xiao-Yan, additional, Xu, Xiaolei, additional, McGrail, Maura, additional, Essner, Jeffrey J, additional, Burgess, Shawn M, additional, Clark, Karl J, additional, and Ekker, Stephen C, additional

Published
2020
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6. Author response: Building the vertebrate codex using the gene breaking protein trap library

Author

Ichino, Noriko, primary, Serres, MaKayla R, additional, Urban, Rhianna M, additional, Urban, Mark D, additional, Treichel, Anthony J, additional, Schaefbauer, Kyle J, additional, Greif, Lauren E, additional, Varshney, Gaurav K, additional, Skuster, Kimberly J, additional, McNulty, Melissa S, additional, Daby, Camden L, additional, Wang, Ying, additional, Liao, Hsin-kai, additional, El-Rass, Suzan, additional, Ding, Yonghe, additional, Liu, Weibin, additional, Anderson, Jennifer L, additional, Wishman, Mark D, additional, Sabharwal, Ankit, additional, Schimmenti, Lisa A, additional, Sivasubbu, Sridhar, additional, Balciunas, Darius, additional, Hammerschmidt, Matthias, additional, Farber, Steven Arthur, additional, Wen, Xiao-Yan, additional, Xu, Xiaolei, additional, McGrail, Maura, additional, Essner, Jeffrey J, additional, Burgess, Shawn M, additional, Clark, Karl J, additional, and Ekker, Stephen C, additional

Published
2020
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7. Novel zebrafish behavioral assay to identify modifiers of the rapid, nongenomic stress response

Author

Lee, Han B., primary, Schwab, Tanya L., additional, Sigafoos, Ashley N., additional, Gauerke, Jennifer L., additional, Krug, Randall G., additional, Serres, MaKayla R., additional, Jacobs, Dakota C., additional, Cotter, Ryan P., additional, Das, Biswadeep, additional, Petersen, Morgan O., additional, Daby, Camden L., additional, Urban, Rhianna M., additional, Berry, Bethany C., additional, and Clark, Karl J., additional

Published
2019
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8. Locomotor response to acute stressors requires hypothalamic-pituitary-interrenal axis activation and glucocorticoid receptor

Author

Lee, Han B., primary, Schwab, Tanya L., additional, Sigafoos, Ashley N., additional, Gauerke, Jennifer L., additional, Krug, Randall G., additional, Serres, MaKayla R., additional, Jacobs, Dakota C., additional, Cotter, Ryan P., additional, Das, Biswadeep, additional, Petersen, Morgan O., additional, Daby, Camden L., additional, Urban, Rhianna M., additional, Berry, Bethany C., additional, and Clark, Karl J., additional

Published
2018
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