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2. Virion proteins of viruses causing hemorrhagic fever with renal syndrome; monoclonal antibody analysis

Author

Hao Lj, Ning Zhou, Dong-liang Yang, and Da-shi Ni

Subjects
Apodemus agrarius, Orthohantavirus, medicine.drug_class, viruses, Blotting, Western, Antibodies, Viral, Monoclonal antibody, Epitope, Epitopes, Mice, Viral Proteins, Antigen, Management of Technology and Innovation, medicine, Animals, Humans, Antigens, Viral, HFRS Viruses, Gel electrophoresis, biology, Virion, Antibodies, Monoclonal, virus diseases, biology.organism_classification, Virology, Rats, Blot, biology.protein, Antibody
Abstract

SDS-polyacrylamide gel electrophoresis and Western blotting analysis by using 9 clones of monoclonal antibodies specific for hemorrhagic fever with renal syndrome (HFRS) viruses were carried out on the virion proteins of 17 strains of HFRS viruses isolated from different areas in Asia and different hosts such as Apodemus agrarius, Rattus norvegicus, domestic cat and HFRS patients. Polypeptide with apparent molecular weight about 50 kitodalton (Kd) could be detected in all 17 strains of HFRS viruses. On this polypeptide there are two different antigenic determinants and one of them might be genus specific.

Published
1989
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