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We conducted this meta-analysis of randomized controlled trials (RCTs) to compare the efficacy of different doses of intravenous tranexamic acid (TXA) in spinal surgery.We searched relevant academic articles from PubMed, Embase, the Cochrane Library, and CNKI. Two reviewers independently selected studies, assessed quality, extracted data, and evaluated the risk of bias. RevMan 5.4 was used for data analysis.Ten randomized controlled trials (RCTs) met the inclusion criteria and were identified, including 740 patients. According to the different dose regimens of intravenous TXA, the included studies' patients were divided into the high dose of intravenous TXA group and the low dose of intravenous TXA group. Compared with the low-dose group, the high-dose group can reduce the intraoperative blood loss (MD = - 100.87, 95% CI: [- 147.81, - 53.92], P 0.0001). For the postoperative Hb and HCT, the high-dose group can separately maintain 4.54 g/dL (MD = 4.54, 95% CI: [2.08, 6.99], P = 0.003) and 1.27% (MD = 1.27, 95% CI: [0.59, 1.94], P = 0.0002). There were no statistically significant differences in total blood loss, preoperative Hb and HCT, operative time, and blood transfusion rate between the high-dose group and the low-dose group.Based on the present meta-analysis, compared with the low-dose of intravenous TXA in spinal surgery, the high dose of intravenous TXA decreases the intraoperative blood loss and preserves higher postoperative Hb and HCT levels without increasing the operative time and blood transfusion rate.

PDF file, 348KB, Figure S1. Synergy between PI3K and HDAC pathway inhibition. Figure S2. CUDC-907 increases acetylation of histone H3 in cultured cancer cells. Figure S3. CUDC-907 increases tubulin acetylation in cultured cancer cells. Figure S4. CUDC-907 increases p53 acetylation in cultured cancer cells. Figure S5. CUDC-907 increases p21 levels in cultured cancer cells. Figure S6. CUDC-907 induces the accumulation of activated caspases-3 and -7. Figure S7. CUDC-907 induces the accumulation of annexin V binding sites. Figure S8. CUDC-907 induces a G2/M cell cycle arrest. Figure S9. CUDC-907 is orally bioavailable and shows intratumoral accumulation.

Purpose: Given that histone deacetylase (HDAC) inhibitors are known to induce multiple epigenetic modifications affecting signaling networks and act synergistically with phosphatidylinositol 3-kinase (PI3K) inhibitors, we developed a strategy to simultaneously inhibit HDACs and PI3K in cancer cells.Experimental Design: We constructed dual-acting inhibitors by incorporating HDAC inhibitory functionality into a PI3K inhibitor pharmacophore. CUDC-907, a development candidate selected from these dual inhibitors, was evaluated in vitro and in vivo to determine its pharmacologic properties, anticancer activity, and mechanism of action.Results: CUDC-907 potently inhibits class I PI3Ks as well as classes I and II HDAC enzymes. Through its integrated HDAC inhibitory activity, CUDC-907 durably inhibits the PI3K-AKT-mTOR pathway and compensatory signaling molecules such as RAF, MEK, MAPK, and STAT-3, as well as upstream receptor tyrosine kinases. CUDC-907 shows greater growth inhibition and proapoptotic activity than single-target PI3K or HDAC inhibitors in both cultured and implanted cancer cells.Conclusions: CUDC-907 may offer improved therapeutic benefits through simultaneous, sustained disruption of multiple oncogenic signaling networks. Clin Cancer Res; 18(15); 4104–13. ©2012 AACR.

Both computational chemistry and molecular simulations are methods of computing molecules through computer algorithms and models to investigate the structure and properties of the molecules of interest. [1] Both are methods to study the properties and structures of molecules under conditions that can be studied without the need for experiments. Since the outbreak of the new crown pneumonia epidemic, and especially since the large-scale outbreak of the COVID-19 in late 2022 in mainland China, the demand for febrile and analgesic drugs by the general population has increased, and the understanding and study of these drugs has become more important. However, the wide variety of drugs and the complex and demanding requirements of the experimental environment have hindered the understanding of these drugs. In this paper, I will perform molecular simulations of the major components of these drugs using computational chemistry to investigate the various chemical properties of these major components in a cost-effective manner.

The newly developed RECoverable Autonomous Sonde (RECAS) allows sampling and analysis of subglacial water while the subglacial lake remains isolated from the surface. The sonde was successfully tested in East Antarctica during the 2021–2022 field season: it reached the ice-sheet base at a depth of 200.3 m, sampled basal meltwater and measured its pressure, temperature, pH and conductivity and returned to the ice surface. The average downward penetration rate was 1.85 m h−1, and the average upward penetration rate was 2.94 m h−1. The successful test of a self-melting, recoverable probe in Antarctica marks further progress towards the clean exploration of subglacial water bodies.

Subglacial lake exploration is of great interest to the science community. RECoverable Autonomous Sonde (RECAS) provides an exploration tool to measure and sample subglacial lake environments while the subglacial lake remains isolated from the glacier surface and atmosphere. This paper presents an electronic control system design of 200 m prototype of RECAS. The proposed electronic control system consists of a surface system, a downhole control system, and a power transfer and communication system. The downhole control system is the core element of RECAS, and is responsible for sonde status monitoring, sonde motion control, subglacial water sampling and in situ analysis. A custom RS485 temperature sensor was developed to cater for the limited size and depth requirements of the system. We adopted a humidity-based measurement to monitor for a housing leak. This condition is because standard leak detection monitoring of water conductivity may be inapplicable to pure ice in Antarctica. A water sampler control board was designed to control the samplers and monitor the on/off state. A high-definition camera system with built-in storage and self-heating ability was designed to perform the video recording in the subglacial lake. The proposed electronic control system is proven effective after a series of tests.

In recent years radar polarimetry has re-surfaced as an ideal tool to determine ice-fabric patterns and linked mechanical ice anisotropy. The leap forward was facilitated by coherent data processing often collected by phase-sensitive Radio-Echo-Sounding (pRES) systems at fixed locations. The polarimetric response can either be synthesized from a set of quad-polarimetric measurements or obtained by manually rotating the antennas. Specifics of the data collection in the field varied between the different surveys, and no set of best practices has yet emerged. Here we present a systematic study that includes more than fifty different combinations of how polarimetric data can be acquired, including:different distances between the transmitter and receiver (2, 4 and 8 m) different combinations in polarization orientation (22.5 deg) a comparison between discrete full azimuthal data collected every 22.5 degrees and synthesized data collected in a quad-pole setup the effect of 180-degree polarization orientation on repeat measurements, e.g., basal melt rate and polarimetric analysis, e.g., coherence phase definition of Horizontal (H) and Vertical (V) orientation is pRES antenna setup and its impact on synthesizing and analyzing data 90-degree fabric orientation ambiguity in polarimetric data This study aims to provide best practices, considering that observation time in the field is limited. Ideally, this will lead to a unified setup and nomenclature, facilitating better compatibility from data collected by different groups on ice sheets, shelves, and glaciers.

A series of new synthetic armored cables were developed and tested to ensure that they were suitable for use with the RECoverable Autonomous Sonde (RECAS), which is a newly designed freezing-in thermal ice probe. The final version of the cable consists of two concentric conductors that can be used as the power and signal lines. Two polyfluoroalkoxy jackets are used for electrical insulation (one for insulation between conductors, and the other for insulation of the outer conductor). The outer insulation layer is coated by polyurethane jacket to seal the connections between the cable and electrical units. The 0.65 mm thick strength member is made from aramid fibers woven together. To hold these aramid fibers in place, a sheathing layer was produced from a polyamide fabric cover net. The outer diameter of the final version of the cable is ~6.1 mm. The permissible bending radius is as low as 17–20 mm. The maximal breaking force under straight tension is ~12.2 kN. The cable weight is only ~0.061 kg m−1. The mechanical and electrical properties and environmental suitability of the cable were determined through laboratory testing and joint testing with the probe.

The Antarctic subglacial drilling rig (ASDR) is designed to recover 105 mm-diameter ice cores up to 1400 m depth and 41.5 mm-diameter bedrock cores up to 2 m in length. In order to ensure safe and convenient drilling, drilling auxiliaries are designed to support fieldwork and servicing. These auxiliaries are subdivided into several systems for power supply, drill tripping in the borehole, ice core and chip processing, and drill servicing and maintenance. The required equipment also includes two generators, a drilling winch with a cable, logging winch with a cable, control desk, pipe handler with a fixed clamp, chip chamber vibrator, centrifuge, emergency devices and fitting and electrical tools. Additionally, several environmental protective measures such as a new liquid-tight casing with a thermal casing shoe and a bailing device for recovering drilling fluid from the borehole were designed. Most of the auxiliaries were tested during the summer of 2018–2019 near Zhongshan Station, East Antarctica while drilling to the bedrock to a depth of 198 m.

Drilling to the bedrock of ice sheets and glaciers offers unique opportunities for examining the processes occurring in the bed. Basal and subglacial materials contain important paleoclimatic and paleoenvironmental records and provide a unique habitat for life; they offer significant information regarding the sediment deformation beneath glaciers and its effects on the subglacial hydraulic system and geology. The newly developed and tested Antarctic subglacial drilling rig (ASDR) is designed to recover ice and bedrock core samples from depths of up to 1400 m. All of the drilling equipment is installed inside a movable, sledge-mounted, temperature-controlled and wind-protected drilling shelter and workshop. To facilitate helicopter unloading of the research vessel, the shelter and workshop can be disassembled, with individual parts weighing

In many cases, the efficiency and safety of a drilling project depend on the reliability of the electrical and electronic control system, as the process progresses without visual access of the operator. The electrical and electronic system provides and regulates the power supply for the drill, collects and monitors the drill data during the whole operating process, and sends and receives the control instructions and feedback signals. The entire system is composed of the surface, borehole and software subsystems. The surface subsystem serves for operating the drilling process, transmitting the drilling and environmental data, and supplying power for the drill motor and downhole control system. The borehole subsystem is generally intended for borehole data acquisition, drill motor control, power regulation and communication. The software subsystem is designed for human–computer interaction, data processing and storage, and programming of signal acquisition and transmission of data. The control system of Antarctic subglacial drilling rig was tested during the 2018–2019 summer season near Zhongshan Station, East Antarctica, in the course of drilling to the bedrock at a depth of 198 m. It exhibited a steady and efficient performance without significant system failures.

A new, modified version of the cable-suspended Ice and Bedrock Electromechanical Drill (IBED) was designed for drilling in firn, ice, debris-rich ice and rock. The upper part of the drill is almost the same for all drill variants and comprises four sections: cable termination, a slip-ring section, an antitorque system and an electronic pressure chamber. The lower part of the IBED comprises an auger core barrel, reamers, a core barrel for ice/debris-ice drilling and a conventional geological single-tube core barrel or custom-made double-tube core barrel. First, the short and full-scale field versions of the IBED were tested at an outdoor testing stand and a testing facility with a 12.5 m-deep ice well. Then, in the 2018–2019 summer season, the IBED was tested in the field at a site ~12 km south of Zhongshan Station, East Antarctica, and a ~6 cm bedrock core was recovered from a 198 m-deep borehole. A total of 18 d was required to penetrate the ice sheet. The retrieved core samples of blue ice, basal ice and bedrock provided valuable information regarding the Earth's paleo-environment.

Mustard gas poses a substantial concern for homeland security, and the development of sensors for facile, rapid, highly sensitive, and selective detection of a mustard gas simulant is much desirabl...

To develop effective alternatives to natural enzymes, it is crucial to develop nanozymes that are economical, resource efficient, and environmentally conscious. Carbon nanomaterials that have enzyme-like activities have been extensively developed as substitutes for traditional enzymes.Carbide-derived carbons (CDCs) were directly synthesizedCDCs showed bienzyme-like activities similar to that of peroxidase and superoxide dismutase. We systematically studied the dependence of CDC enzyme-like activity on different electrolytes and electrolysis times to confirm activity dependence on CDC content. Additionally, the synthesis mechanism and CDC applicability were elaborated and demonstrated, respectively.The demonstrated synthesis strategy eliminates tedious intercalation and delamination centrifugation steps and avoids using high concentrations of HF, high temperatures, and halogen gases. This study paves the way for designing two-dimensional material-based nanocatalysts for nanoenzyme and other applications.

Six piperazine derivatives 6a-f containing 1,4-benzodioxan moiety have been synthesized and characterized by 1H NMR, ESI-MS and elemental analysis. The structure of 6d was further confirmed by single crystal X-ray diffraction. All these novel compounds were screened for their in vivo anti-inflammatory activity employing classical para-xylene-induced mice ear-swelling model. The results revealed that most of the target compounds showed significant anti-inflammatory activities, especially compound 6a with ortho-substituted methoxy group on the phenylpiperazine ring exhibited the best activity among the designed compounds.

To prepare a tissue-engineered pulmonary valved conduit (PVC) with good tensile strength and biocompatibility. Sixty adult porcine PVCs were used to determine the optimal decellularization time. Five juvenile porcine decellularized PVCs and five juvenile porcine crosslinked PVCs were subsequently prepared according to the optimized decellularization and crosslinking methods. All PVCs were implanted into juvenile sheep for 8 months and then were harvested for staining. With a low concentration of detergent (0.25% Triton X-100+0.25% sodium deoxycholate), the decellularization effect on porcine PVCs was complete by 24 hours, and there was minimal damage to the matrix. Gelatin embedding and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) crosslinking improved the biomechanical properties of decellularized PVCs and reduced their immunogenicity. After implantation, the diameter and thickness of the PVCs in the decellularized and crosslinked groups increased significantly. In both groups, the conduits were unobstructed, with soft and smooth inner walls and without thrombosis, ulceration or neoplasia. The valves slightly degenerated with mild to moderate regurgitation. CD31-positive endothelial cells were visible on the inner surface of the conduits and valves. Scattered smooth muscle actin-positive cells were found in the middle layer of the conduit. The percentage of CD4- and CD68-positive cells and the calcium content were highest in decellularized porcine PVCs and lowest in ovine PVCs. The percentage of the matrix that was laminin-positive in decellularized and crosslinked porcine PVCs was lower than it was in ovine PVCs. Gelatin-embedded and EDC-crosslinked porcine PVCs can be "hosted" in sheep, with good biocompatibility, growth potential, and reduced calcification.

Shallow hot-water systems are well-known ice-drilling devices used to create holes for temperature measurements and monitoring of glacier dynamics, basal sliding, and englacial water-pressure. A shallow hot-water ice drill system designed at Jilin University is based on a commercial high-pressure washer, Karcher HDS 6/14C, that can deliver water at a temperature in the range of 80–155° C, a flow of 4–10 L/min and a pressure as high as 14 MPa. The system also includes a mast with a sheave on the top and a reel with a 100-m long rubber hose; a 2-m long, 60-mm diameter drill stem; a control and measuring system; sleds; a submersible pump; a return hose; a water tank with an affiliate heating unit; and a small 2-kW generator. Theoretical estimations were performed to predict the rate of penetration and mean diameter of the melted borehole. Experiments with a shallow hot-water drill were carried out in an ice drill testing facility to determine the relationship between the rate of penetration/borehole diameter and the diameter and type of nozzle, hot water temperature, and flowrate. Under a hot water flowrate of 10 L/min with a temperature of 60° C, the 1.8-mm and 2-mm nozzles created 98–114-mm diameter boreholes at a penetration rate of 34–37 m/h, while a 2.5-mm nozzle produced a 146–156-mm borehole at rate of 26 m/h. The deviation between the experimental data and theoretical estimations did not exceed 7%.

Electric thermal drills are more advantageous than electromechanical drills in temperate, near-temperate, and polythermal glaciers because they can avoid problems arising from refreezing of wet chips, which causes drills to become stuck in the borehole. When the refreezing rate of meltwater in borehole is expected to be too high and there is no considerable englacial water flow, thermal drills with meltwater removal system are optional for open-hole shallow (200–300 m) ice coring. To reach a sufficiently high rate of penetration of approximately 6–7 m h −1 , the power density of thermal head should be maintained in the range of 100–110 W cm −2 , which can be provided by tubular elements cast integrally with an aluminum or copper annulus. To remove meltwater via air reverse circulation, thermal drills can be equipped with a small blower. The safest and most even mode of water removal is lifting in the form of water film on the wall of air sucking tubes. The maximum water removal rate using a single water-lifting pipe via air reverse circulation created by a blower with a sucking power of 110 airwatts was ∼0.35 L min −1 . Assuming a penetration rate of 6–7 m h −1 and the outer and inner diameters of 135 and 110 mm, respectively, of the drill head, the meltwater should be removed at a rate not less than 0.8–1.0 L min −1 . In this case, at least three water-lifting pipes should be used in the drill.