301 results on '"Da Costa, Jc"'
Search Results
2. Cleaner production applications for coal utilisation
- Author
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National Environment Conference (2003 : Brisbane, Qld.), Pagan, RJ, Prasad, P, Diniz da Costa, JC, and van Berkel, R
- Published
- 2003
3. Novel composite membranes for gas separation
- Author
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National Environment Conference (2003 : Brisbane, Qld.), Duke, MC, Hogarth, WHJ, Lu, GQ, Diniz da Costa, JC, Petch, M, and Gray, P
- Published
- 2003
4. Effect of Cinacalcet on Cardiovascular Disease in Patients Undergoing Dialysis
- Author
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Chertow GM, Block GA, Correa-Rotter R, Drüeke TB, Floege J, Goodman WG, Herzog CA, Kubo Y, London GM, Mahaffey KW, Mix TC, Moe SM, Trotman ML, Wheeler DC, Parfrey PS., Evolve Team, Chertow G, Parfrey P, Block G, Drüeke T, Goodman W, Herzog C, London G, Mahaffey K, Moe S, Wheeler D, Hennekens C, Baigent C, Brown W, O'Brien P, Anderson S, Hoel J, Szczech L, Patel U, Wampole J, Pun P, Felker M, Inrig J, Shah S, Hernandez A, Patel C, Brennan M, Albizem M, Capper E, Cauchi L, Cheng S, Dehmel B, Dhami K, Durham C, Francioni M, Gadd S, Goodman B, Guimaraes L, Grey N, Hamlin R, Harris C, Harris E, Heavey S, Heiges T, Heiser D, Jaeger P, James M, James P, Karimi S, Kewalramani R, Kraszewski A, Liang J, Maguire J, McCormick K, McFarlane K, Mix C, Modafferi D, Prathikanti R, Ryan C, Santiago N, Schumacher J, Seder C, Shahinfar S, Soares B, Stolman D, Tisher C, Trotman M, Tseng S, Ulias G, Unger P, Vyshenskaya A, Walsh L, White C, Wilde K, Santos J, Zarazaga C, Marin I, Garrote N, Cusumano A, Penalba N, Del Valle E, Juncos L, Saye J, Lef L, Altobelli V, Petraglia G, Rosa-Diez G, Douthat W, Lobo J, Gallart C, Lafalla A, Diez G, Linares B, Lopez N, Ramirez N, Gonzalez R, Valtuille R, Beresan H, Hermida O, Rudolf G, Marchetta N, Rano M, Ramirez M, Garcia N, Gillies A, Jones B, Pedagogos E, Walker R, Talaulikar G, Bannister K, Suranyi M, Kark A, Roger S, Kerr P, Disney A, Mount P, Fraenkel M, Mathew M, Fassett R, Jose M, Hawley C, Lonergan M, Mackie J, Ferrari P, Menahem S, Sabto J, Hutchison B, Langham R, Pollock C, Holzer H, Oberbauer R, Arias I, Graf H, Mayer G, Lhotta K, Neyer U, Klauser-Braun R, Hoerl W, Horn S, Kovarik J, Kramar R, Eigner M, Dhaene M, Billiouw J, De Meester J, Warling X, Cambier-Dwelschauwers P, Evenepoel P, Daelemans R, Dratwa M, Maes B, Stolear J, Dejagere T, Vanwalleghem J, Bouman K, Jadoul M, Peeters J, Vanholder R, Tielemans C, Donck J, Almeida F, de Oliveira J, Burdmann E, Garcia V, Thome F, Deboni L, Bregman R, Lugon J, Araújo S, Ferreira Filho S, Daher Ede F, Baptista M, Carvalho A, d'Avila D, Moyses Neto M, Yu L, Bastos M, Lacativa P, Jorgetti V, Romão Ede A, da Costa JC, Pecoits Filho R, Gordan P, Salgado N, Araújo M, Coelho S, Oliveira I, Moysés R, Vasconcellos L, Batista P, Gross J, Pedrosa A, Cournoyer S, LeBlanc M, Chow S, Karunakaran S, Wong G, Tobe S, Desmeules S, Zimmerman D, Murphy S, Montambault P, Donnelly S, MacRae J, Culleton B, Soroka S, Rabbat C, Jindal K, Vasilevsky M, Michaud M, Wijeyesinghe E, Zacharias J, Lok C, Muirhead N, Verrelli M, Da Roza G, Sapir D, Olgaard K, Daugaard H, Brandi L, Jensen P, Boulechfar H, Ang K, Simon P, Rieu P, Brunet P, Touchard G, Torres P, Combe C, Durrbach A, Ortiz J, Hannedouche T, Vela C, Lionet A, Ryckelynck P, Zaoui P, Choukroun G, Fessi H, Lang P, Stroumza P, Joly D, Mousson C, Laville M, Dellanna F, Erley C, Braun J, Rambausek M, Riegel W, Klingberg M, Schwertfeger E, Wizemann V, Eckardt K, Reichel H, Passauer J, Hübel E, Frischmuth N, Liebl R, Fiedler R, Schwenger V, Voßkühler A, Kunzendorf U, Renders L, Rattensberger D, Rump L, Ketteler M, Neumayer H, Zantvoort F, Stahl R, Ladanyi E, Braun B, Kulcsar I, Mezei I, Csiky B, Rikker C, Arkossy O, Berta K, Szegedi J, Major L, Ferenczi S, Fekete A, Szabo T, Zakar G, Wagner G, Erdelyine S, Borbas B, Eustace J, Reddan D, Capasso G, Locatelli F, Villa G, Cozzolino M, Brancaccio D, Messa P, Bolasco P, Ricciardi B, Malberti F, Moriero E, Cannella G, Ortalda V, Stefoni S, Frascà G, Cappelli G, Albertazzi A, Zoccali C, Farina M, Elli A, Avella F, Ondei P, Mingardi G, Errico R, Losito A, Di Giulio S, Pertosa G, Schena F, Grandaliano G, Gesualdo L, Auricchio M, Bochicchio-Ricardelli T, Correa-Rotter J, Verástegui F, Peña J, Wong A, Cruz-Valdez J, Zamora M, Solis M, Diaz M, Flores M, Sandoval E, van den Dorpel M, Brink H, Van Kuijk W, Vermeij C, Gregoor P, Hagen E, van der Sande F, Klinger M, Nowicki M, Muszytowski M, Bidas K, Bentkowski W, Wiecek A, Ksiazek A, Marczewski K, Ostrowski M, Switalski M, Sulowicz W, Matuszkiewicz-Rowinska J, Mysliwiec M, Durlik M, Rutkowski B, Macario F, Carvalho B, Frazao J, Machado D, Weigert A, Andrusev A, Khrustalev O, Zemtchenkov A, Gurevich K, Staroselsky K, Khadikova N, Rozhinskaya L, Timokhovskaya G, Strokov A, Balkarova O, Ermolenko V, Kolmakova E, Komandenko M, Timofeev M, Shilo V, Shostka G, Smirnov A, Anashkin V, Volgina G, Domashenko O, Gurevich A, Perlin D, García J, Ribes E, Piera E, Lucas M, Galicia M, Prados M, González M, Romero R, de Francisco ÁM, Montenegro J, Santiago C, García F, de La Ossa J, Arrieta J, Pons J, Martín-Malo A, Amigó J, Cases A, Sterner G, Jensen G, Wikström B, Jacobson S, Lund U, Weiss L, Ståhl A, von Albertini B, Burnier M, Meier P, Martin P, Uehlinger D, Dickenmann M, Yaqoob M, Zehnder D, Kalra P, Padmanabhan N, Roe S, Eadington D, Pritchard N, Hutchison A, Davies S, Wilkie M, Davies M, Pai P, Swift P, Kwan J, Goldsmith D, Tomson C, Stratton J, Dasgupta I, Sarkar S, Moustafa M, Gandhi K, Jamal A, Galindo-Ramos E, Tuazon J, Batlle D, Tucker K, Schiller-Moran B, Assefi A, Martinez C, Samuels L, Goldman J, Cangiano-Rivera J, Darwish R, Lee M, Topf J, Kapatkin K, Baer H, Kopelman R, Acharya M, Tharpe D, Bernardo M, Nader P, Guzman-Rivera J, Pergola P, Sekkarie M, Alas E, Zager P, Liss K, Navarro J, Roppolo M, Denu-Ciocca C, Kshirsagar A, El Khatib M, Kant K, Scott D, Murthyr B, Finkelstein F, Keightley G, McCrary R, Pitone J, Cavalieri T, Tsang A, Pellegrino B, Schmidt R, Ahmad S, Brown C, Friedman E, Mittman N, Fadem S, Shapiro W, Reddy M, Goldberger S, Woredekal Y, Agarwal A, Anger M, Haque M, Chidester P, Kohli R, Rubinstein S, Newman G, Gladish R, Ayodeji O, Soman S, Sprague S, Hunt N, Gehr T, Rizk D, Warnock D, Polack D, Pahl M, Fischer D, Dreyer P, James G, Husserl F, Rogers T, Raff A, Sedor J, Silver M, Smith M, Steinberg S, DelGiorno T, Jones E, Cunha P, Cheng J, Pogue V, Blumenthal S, Brown E, Charytan C, Buerkert J, Cook M, Felsenfeld A, Tareen N, Gupta A, Herman T, Diamond S, Hura C, Laski M, MacLaurin J, Plumb T, Brosnahan G, Kumar J, Henriquez M, Poole C, Osanloo E, Matalon A, Sholer C, Arfeen S, Azer M, Belledonne M, Gross M, Dunnigan E, McConnell K, Becker B, Rigolosi R, Spiegel D, Stegman M, Patak R, Streja D, Ranjit U, Youell T, Wooldridge T, Stafford C, Cottiero R, Weinberg M, Schonefeld M, Shahmir E, Hazzan A, Ashfaq A, Bhandari K, Cleveland W, Culpepper M, Golden J, Lai L, Lien Y, Lorica V, Robertson J, Malireddi K, Morse S, Thakur V, Israelit A, Raguram P, Alfred H, Weise W, Al-Saghir F, El Shahawy M, Rastogi A, Nissenson A, Kopyt N, Lynn R, Lea J, McClellan W, Teredesai P, Ong S, Tolkan S, Sugihara J, Minga T, Mehrotra R, Minasian R, Bhatia D, Specter R, Capelli J, Sidhu P, Dalal S, Dykes P, Khan M, Rahim F, Saklayen M, Thomas A, Michael B, Torres M, Al-Bander H, Murray B, Abukurah A, Gupta B, Nosrati S, Raja R, Zeig S, Braun M, Amatya A, Endsley J, Sharon Z, Dolson G, Dumler F, Ntoso K, Rosansky S, Kumar N, Gura V, Thompson N, Goldfarb D, Halligan R, Middleton J, Widerhorn A, Arbeit L, Arruda J, Crouch T, Friedman L, Khokhar S, Mittleman J, Light P, Taparia B, West C, Cotton J, Dhingra R, Kleinman L, Arif F, Lew S, Nammour T, Sterrett J, Williams M, Ramirez J, Rubin J, McCarthy J, Noble S, Chaffin M, Rekhi A., Chertow, Gm, Block, Ga, Correa-Rotter, R, Drüeke, Tb, Floege, J, Goodman, Wg, Herzog, Ca, Kubo, Y, London, Gm, Mahaffey, Kw, Mix, Tc, Moe, Sm, Trotman, Ml, Wheeler, Dc, Parfrey, Ps., Evolve, Team, Chertow, G, Parfrey, P, Block, G, Drüeke, T, Goodman, W, Herzog, C, London, G, Mahaffey, K, Moe, S, Wheeler, D, Hennekens, C, Baigent, C, Brown, W, O'Brien, P, Anderson, S, Hoel, J, Szczech, L, Patel, U, Wampole, J, Pun, P, Felker, M, Inrig, J, Shah, S, Hernandez, A, Patel, C, Brennan, M, Albizem, M, Capper, E, Cauchi, L, Cheng, S, Dehmel, B, Dhami, K, Durham, C, Francioni, M, Gadd, S, Goodman, B, Guimaraes, L, Grey, N, Hamlin, R, Harris, C, Harris, E, Heavey, S, Heiges, T, Heiser, D, Jaeger, P, James, M, James, P, Karimi, S, Kewalramani, R, Kraszewski, A, Liang, J, Maguire, J, Mccormick, K, Mcfarlane, K, Mix, C, Modafferi, D, Prathikanti, R, Ryan, C, Santiago, N, Schumacher, J, Seder, C, Shahinfar, S, Soares, B, Stolman, D, Tisher, C, Trotman, M, Tseng, S, Ulias, G, Unger, P, Vyshenskaya, A, Walsh, L, White, C, Wilde, K, Santos, J, Zarazaga, C, Marin, I, Garrote, N, Cusumano, A, Penalba, N, Del Valle, E, Juncos, L, Saye, J, Lef, L, Altobelli, V, Petraglia, G, Rosa-Diez, G, Douthat, W, Lobo, J, Gallart, C, Lafalla, A, Diez, G, Linares, B, Lopez, N, Ramirez, N, Gonzalez, R, Valtuille, R, Beresan, H, Hermida, O, Rudolf, G, Marchetta, N, Rano, M, Ramirez, M, Garcia, N, Gillies, A, Jones, B, Pedagogos, E, Walker, R, Talaulikar, G, Bannister, K, Suranyi, M, Kark, A, Roger, S, Kerr, P, Disney, A, Mount, P, Fraenkel, M, Mathew, M, Fassett, R, Jose, M, Hawley, C, Lonergan, M, Mackie, J, Ferrari, P, Menahem, S, Sabto, J, Hutchison, B, Langham, R, Pollock, C, Holzer, H, Oberbauer, R, Arias, I, Graf, H, Mayer, G, Lhotta, K, Neyer, U, Klauser-Braun, R, Hoerl, W, Horn, S, Kovarik, J, Kramar, R, Eigner, M, Dhaene, M, Billiouw, J, De Meester, J, Warling, X, Cambier-Dwelschauwers, P, Evenepoel, P, Daelemans, R, Dratwa, M, Maes, B, Stolear, J, Dejagere, T, Vanwalleghem, J, Bouman, K, Jadoul, M, Peeters, J, Vanholder, R, Tielemans, C, Donck, J, Almeida, F, de Oliveira, J, Burdmann, E, Garcia, V, Thome, F, Deboni, L, Bregman, R, Lugon, J, Araújo, S, Ferreira Filho, S, Daher Ede, F, Baptista, M, Carvalho, A, D'Avila, D, Moyses Neto, M, Yu, L, Bastos, M, Lacativa, P, Jorgetti, V, Romão Ede, A, da Costa, Jc, Pecoits Filho, R, Gordan, P, Salgado, N, Araújo, M, Coelho, S, Oliveira, I, Moysés, R, Vasconcellos, L, Batista, P, Gross, J, Pedrosa, A, Cournoyer, S, Leblanc, M, Chow, S, Karunakaran, S, Wong, G, Tobe, S, Desmeules, S, Zimmerman, D, Murphy, S, Montambault, P, Donnelly, S, Macrae, J, Culleton, B, Soroka, S, Rabbat, C, Jindal, K, Vasilevsky, M, Michaud, M, Wijeyesinghe, E, Zacharias, J, Lok, C, Muirhead, N, Verrelli, M, Da Roza, G, Sapir, D, Olgaard, K, Daugaard, H, Brandi, L, Jensen, P, Boulechfar, H, Ang, K, Simon, P, Rieu, P, Brunet, P, Touchard, G, Torres, P, Combe, C, Durrbach, A, Ortiz, J, Hannedouche, T, Vela, C, Lionet, A, Ryckelynck, P, Zaoui, P, Choukroun, G, Fessi, H, Lang, P, Stroumza, P, Joly, D, Mousson, C, Laville, M, Dellanna, F, Erley, C, Braun, J, Rambausek, M, Riegel, W, Klingberg, M, Schwertfeger, E, Wizemann, V, Eckardt, K, Reichel, H, Passauer, J, Hübel, E, Frischmuth, N, Liebl, R, Fiedler, R, Schwenger, V, Voßkühler, A, Kunzendorf, U, Renders, L, Rattensberger, D, Rump, L, Ketteler, M, Neumayer, H, Zantvoort, F, Stahl, R, Ladanyi, E, Braun, B, Kulcsar, I, Mezei, I, Csiky, B, Rikker, C, Arkossy, O, Berta, K, Szegedi, J, Major, L, Ferenczi, S, Fekete, A, Szabo, T, Zakar, G, Wagner, G, Erdelyine, S, Borbas, B, Eustace, J, Reddan, D, Capasso, G, Locatelli, F, Villa, G, Cozzolino, M, Brancaccio, D, Messa, P, Bolasco, P, Ricciardi, B, Malberti, F, Moriero, E, Cannella, G, Ortalda, V, Stefoni, S, Frascà, G, Cappelli, G, Albertazzi, A, Zoccali, C, Farina, M, Elli, A, Avella, F, Ondei, P, Mingardi, G, Errico, R, Losito, A, Di Giulio, S, Pertosa, G, Schena, F, Grandaliano, G, Gesualdo, L, Auricchio, M, Bochicchio-Ricardelli, T, Correa-Rotter, J, Verástegui, F, Peña, J, Wong, A, Cruz-Valdez, J, Zamora, M, Solis, M, Diaz, M, Flores, M, Sandoval, E, van den Dorpel, M, Brink, H, Van Kuijk, W, Vermeij, C, Gregoor, P, Hagen, E, van der Sande, F, Klinger, M, Nowicki, M, Muszytowski, M, Bidas, K, Bentkowski, W, Wiecek, A, Ksiazek, A, Marczewski, K, Ostrowski, M, Switalski, M, Sulowicz, W, Matuszkiewicz-Rowinska, J, Mysliwiec, M, Durlik, M, Rutkowski, B, Macario, F, Carvalho, B, Frazao, J, Machado, D, Weigert, A, Andrusev, A, Khrustalev, O, Zemtchenkov, A, Gurevich, K, Staroselsky, K, Khadikova, N, Rozhinskaya, L, Timokhovskaya, G, Strokov, A, Balkarova, O, Ermolenko, V, Kolmakova, E, Komandenko, M, Timofeev, M, Shilo, V, Shostka, G, Smirnov, A, Anashkin, V, Volgina, G, Domashenko, O, Gurevich, A, Perlin, D, García, J, Ribes, E, Piera, E, Lucas, M, Galicia, M, Prados, M, González, M, Romero, R, de Francisco, Ám, Montenegro, J, Santiago, C, García, F, de La Ossa, J, Arrieta, J, Pons, J, Martín-Malo, A, Amigó, J, Cases, A, Sterner, G, Jensen, G, Wikström, B, Jacobson, S, Lund, U, Weiss, L, Ståhl, A, von Albertini, B, Burnier, M, Meier, P, Martin, P, Uehlinger, D, Dickenmann, M, Yaqoob, M, Zehnder, D, Kalra, P, Padmanabhan, N, Roe, S, Eadington, D, Pritchard, N, Hutchison, A, Davies, S, Wilkie, M, Davies, M, Pai, P, Swift, P, Kwan, J, Goldsmith, D, Tomson, C, Stratton, J, Dasgupta, I, Sarkar, S, Moustafa, M, Gandhi, K, Jamal, A, Galindo-Ramos, E, Tuazon, J, Batlle, D, Tucker, K, Schiller-Moran, B, Assefi, A, Martinez, C, Samuels, L, Goldman, J, Cangiano-Rivera, J, Darwish, R, Lee, M, Topf, J, Kapatkin, K, Baer, H, Kopelman, R, Acharya, M, Tharpe, D, Bernardo, M, Nader, P, Guzman-Rivera, J, Pergola, P, Sekkarie, M, Alas, E, Zager, P, Liss, K, Navarro, J, Roppolo, M, Denu-Ciocca, C, Kshirsagar, A, El Khatib, M, Kant, K, Scott, D, Murthyr, B, Finkelstein, F, Keightley, G, Mccrary, R, Pitone, J, Cavalieri, T, Tsang, A, Pellegrino, B, Schmidt, R, Ahmad, S, Brown, C, Friedman, E, Mittman, N, Fadem, S, Shapiro, W, Reddy, M, Goldberger, S, Woredekal, Y, Agarwal, A, Anger, M, Haque, M, Chidester, P, Kohli, R, Rubinstein, S, Newman, G, Gladish, R, Ayodeji, O, Soman, S, Sprague, S, Hunt, N, Gehr, T, Rizk, D, Warnock, D, Polack, D, Pahl, M, Fischer, D, Dreyer, P, James, G, Husserl, F, Rogers, T, Raff, A, Sedor, J, Silver, M, Smith, M, Steinberg, S, Delgiorno, T, Jones, E, Cunha, P, Cheng, J, Pogue, V, Blumenthal, S, Brown, E, Charytan, C, Buerkert, J, Cook, M, Felsenfeld, A, Tareen, N, Gupta, A, Herman, T, Diamond, S, Hura, C, Laski, M, Maclaurin, J, Plumb, T, Brosnahan, G, Kumar, J, Henriquez, M, Poole, C, Osanloo, E, Matalon, A, Sholer, C, Arfeen, S, Azer, M, Belledonne, M, Gross, M, Dunnigan, E, Mcconnell, K, Becker, B, Rigolosi, R, Spiegel, D, Stegman, M, Patak, R, Streja, D, Ranjit, U, Youell, T, Wooldridge, T, Stafford, C, Cottiero, R, Weinberg, M, Schonefeld, M, Shahmir, E, Hazzan, A, Ashfaq, A, Bhandari, K, Cleveland, W, Culpepper, M, Golden, J, Lai, L, Lien, Y, Lorica, V, Robertson, J, Malireddi, K, Morse, S, Thakur, V, Israelit, A, Raguram, P, Alfred, H, Weise, W, Al-Saghir, F, El Shahawy, M, Rastogi, A, Nissenson, A, Kopyt, N, Lynn, R, Lea, J, Mcclellan, W, Teredesai, P, Ong, S, Tolkan, S, Sugihara, J, Minga, T, Mehrotra, R, Minasian, R, Bhatia, D, Specter, R, Capelli, J, Sidhu, P, Dalal, S, Dykes, P, Khan, M, Rahim, F, Saklayen, M, Thomas, A, Michael, B, Torres, M, Al-Bander, H, Murray, B, Abukurah, A, Gupta, B, Nosrati, S, Raja, R, Zeig, S, Braun, M, Amatya, A, Endsley, J, Sharon, Z, Dolson, G, Dumler, F, Ntoso, K, Rosansky, S, Kumar, N, Gura, V, Thompson, N, Goldfarb, D, Halligan, R, Middleton, J, Widerhorn, A, Arbeit, L, Arruda, J, Crouch, T, Friedman, L, Khokhar, S, Mittleman, J, Light, P, Taparia, B, West, C, Cotton, J, Dhingra, R, Kleinman, L, Arif, F, Lew, S, Nammour, T, Sterrett, J, Williams, M, Ramirez, J, Rubin, J, Mccarthy, J, Noble, S, Chaffin, M, and Rekhi, A.
- Subjects
Adult ,Male ,Dialysis ,Cinacalcet ,Cardiovascular Disease ,medicine.medical_specialty ,Calcimimetic ,medicine.medical_treatment ,Naphthalenes ,Coronary artery disease ,Renal Dialysis ,Internal medicine ,Odds Ratio ,medicine ,Humans ,Intensive care medicine ,Aged ,Etelcalcetide ,Hyperparathyroidism ,Hypocalcemia ,business.industry ,General Medicine ,Middle Aged ,medicine.disease ,Intention to Treat Analysis ,Cardiovascular Diseases ,Parathyroid Hormone ,Cinacalcet Hydrochloride ,Kidney Failure, Chronic ,Female ,Hyperparathyroidism, Secondary ,Secondary hyperparathyroidism ,business ,medicine.drug - Abstract
Disorders of mineral metabolism, including secondary hyperparathyroidism, are thought to contribute to extraskeletal (including vascular) calcification among patients with chronic kidney disease. It has been hypothesized that treatment with the calcimimetic agent cinacalcet might reduce the risk of death or nonfatal cardiovascular events in such patients.In this clinical trial, we randomly assigned 3883 patients with moderate-to-severe secondary hyperparathyroidism (median level of intact parathyroid hormone, 693 pg per milliliter [10th to 90th percentile, 363 to 1694]) who were undergoing hemodialysis to receive either cinacalcet or placebo. All patients were eligible to receive conventional therapy, including phosphate binders, vitamin D sterols, or both. The patients were followed for up to 64 months. The primary composite end point was the time until death, myocardial infarction, hospitalization for unstable angina, heart failure, or a peripheral vascular event. The primary analysis was performed on the basis of the intention-to-treat principle.The median duration of study-drug exposure was 21.2 months in the cinacalcet group, versus 17.5 months in the placebo group. The primary composite end point was reached in 938 of 1948 patients (48.2%) in the cinacalcet group and 952 of 1935 patients (49.2%) in the placebo group (relative hazard in the cinacalcet group vs. the placebo group, 0.93; 95% confidence interval, 0.85 to 1.02; P=0.11). Hypocalcemia and gastrointestinal adverse events were significantly more frequent in patients receiving cinacalcet.In an unadjusted intention-to-treat analysis, cinacalcet did not significantly reduce the risk of death or major cardiovascular events in patients with moderate-to-severe secondary hyperparathyroidism who were undergoing dialysis. (Funded by Amgen; EVOLVE ClinicalTrials.gov number, NCT00345839.).
- Published
- 2012
- Full Text
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5. Neuropatologia das epilepsias de difícil controle: estudo de 300 casos consecutivos
- Author
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Eduardo Paglioli, Maria Elisa Calcagnotto, Lígia Maria Barbosa-Coutinho, De Paula Ec, André Palmini, Arlete Hilbig, Paglioli Neto E, and Da Costa Jc
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Hippocampal sclerosis ,Pathology ,medicine.medical_specialty ,business.industry ,Neuroepithelial tumors ,Neuronal migration ,Neuropathology ,medicine.disease ,Temporal lobe ,Epilepsy ,Neurology ,Medicine ,Epilepsy surgery ,Neurology (clinical) ,business - Abstract
Fazemos uma análise das alterações neuropatológicas encontradas em 300 casos consecutivos de cirurgia da epilepsia realizadas durante período de 6 anos. O material foi predominantemente de lobo temporal (70,33%), sendo a esclerose hipocampal o diagnóstico mais frequente (44%), seguido das neoplasias (15%) e dos distúrbios da migração neuronal (10%). Os tumores mais frequentes foram o ganglioglioma (42,22%) e tumor neuroepitelial disembrioplástico (20%). Segue revisão dos diagnósticos mais comuns em epilepsia baseados nessa série e relatados na literatura.
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- 1999
- Full Text
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6. Participação do Mycoplasma pneumoniae na etiologia de infecções respiratórias agudas em Ribeirão Preto, São Paulo, Brasil
- Author
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Alcyone Artioli Machado, Rossini Aj, da Costa Jc, and Couch Rb
- Subjects
Microbiology (medical) ,Mycoplasma pneumoniae ,Pneumonia by Mycoplasma ,Infecções respiratórias ,lcsh:Arctic medicine. Tropical medicine ,lcsh:RC955-962 ,Prevalência do Mycoplasma pneumoniae ,medicine.disease_cause ,Serology ,MEDICINA VETERINÁRIA ,Pneumonias por micoplasma ,Respiratory infection ,Medicine ,Respiratory system ,business.industry ,Prevalence of Mycoplasma pneumoniae ,Mycoplasma ,Complement fixation test ,Infectious Diseases ,Immunology ,Etiology ,Parasitology ,business ,Counterimmunoelectrophoresis - Abstract
Para avaliar a participação etiológica do Mycoplasma pneumoniae em infecções respiratórias agudas, o isolamento dessa bactéria foi tentado em secreções do aparelho respiratório de 64 pacientes (média 24 anos) com quadro respiratório aguda Foi realizada, também, a pesquisa de anticorpos específicos anti-M. pneumoniae através da reação de fixação do complemento (FC) e da reação de contra-imunoeletroforese (CIE). O M. pneumoniae não foi isolado. O diagnóstico presuntivo de infecção pelo M. pneumoniae foi feito pela FC em 3,1% (2/64) e pela CIE em 1,6% (1/64) dos pacientes. Paralelamente, em 200 indivíduos sadios, os mesmos testes sorológicos foram realizados, sendo o índice de positividade de 4% (8/200) pela CIE e de 1% (2/200) pela FC. Apesar das aiscrepâncias observadas entre os dois métodos sorológicos, a FC parece ser indicada para diagnóstico da infecção, sendo a CIE recomendada nas avaliações soroepidemiológicas. Com base nos dados do nosso estudo, a prevalência das infecções respiratórias pelo M. pneumoniae parece ser baixa em nosso meio.Mycoplasma pneumoniae isolation was attempted in respiratory fluids from 64 patients with respiratory infection Complement fixation test (CF) and counter- immunoelectrophoresis (CIE) were used for Mycoplasma antibody detection using the patient sera. Mycoplasma pneumoniae was not isolated. Serologic diagnosis were positives in 3,1% (2/64) by CF test and 1,6% (1/64) by CIE. Serologic tests done in 200 health controls showed 4% (8/200)positives by CIE and 1 % (2/200) by CF. The results showed differences in sensitivity among the serologic tests. CF seems to be more indicated for Mycoplasma infection diagnosis while, CIE could be used for Mycoplasmas serosurveys. The prevalence of Mycoplasma pneumoniae infections was low (3,1%) in the 64 patients during our study period.
- Published
- 1991
7. Copaifera langsdorffii aqueous extract and its galloylquinic acid display gastroprotective activity
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Da Costa, JC, primary, Motta, ED, additional, Lemos, M, additional, and Bastos, JK, additional
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- 2013
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8. Assessment of the efficiency of captive-bolt stunning in cattle and feasibility of associated behavioural signs
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Gouveia, KG, primary, Ferreira, PG, additional, da Costa, JC Roque, additional, Vaz-Pires, P, additional, and da Costa, P Martins, additional
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- 2009
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9. Psychoneuroendocrine characteristics of common obesity clinical subtypes
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Martins, JM, primary, Trinca, A, additional, Afonso, A, additional, Carreiras, F, additional, Falcão, J, additional, Nunes, JS, additional, do Vale, S, additional, and da Costa, JC, additional
- Published
- 2001
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10. Imunidade à rubéola: inquérito soro-epidemiológico em hospital, Estado de São Paulo - Brasil
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Alcyone Artioli Machado, da Costa Jc, and Campos Ad
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Geography ,Immunity ,Environmental health ,Public Health, Environmental and Occupational Health ,medicine ,medicine.disease ,Rubella - Abstract
Os títulos de anticorpos no soro pela reação de inibição da hemaglutinação para rubéola, empregando o caulim para adsorção de beta-lipoproteínas bloqueadores inespecíficos, foram determinados em funcionárias do Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo (Brasil), no período de 1982-1983 e confrontados com idade, cor, unidade hospitalar, local, cargo, tempo de emprego, antecedentes de rubéola ou comunicante na vigência ou não de gravidez. Participaram do estudo 1.886 funcionárias (88,9% de 2.121) tendo títulos com a distribuição: 9,6% @ 0); 87,1% das funcionárias negaram antecedentes de rubéola e destas 73,9% tiveram títulos > 20; 57,6% negaram ser comunicantes e apresentaram títulos ³ 20; em 1,1% que referiram história de rubéola, os títulos foram £20; 97% negaram contacto com rubéola durante a gravidez. Houve somente um caso de malformação congênita após rubéola no primeiro trimestre da gravidez. Das 351 funcionárias sãs, e com títulos ³ 640, em 9,4% demonstrou-se IgM específica. Não foi notada flutuação significativa dos títulos em diferentes amostras em período de observação de até um ano. Conclui-se que a maioria das funcionárias é imune à rubéola (título > 20) independente de quaisquer parâmetros analisados; a presença de IgM específica em algumas funcionárias pode ser compatível com doença subclínica. Este inquérito foi considerado útil na orientação de funcionárias grávidas comunicantes de caso suspeito ou confirmado de rubéola, e para as não-grávidas e não-imunes a indicação da profilaxia pela vacina.
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- 1988
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11. FUEL 214-Hydrogen purification from coal gasification using inorganic membranes in Australia
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Duke, Mikel C., Gopalakrishnan, Suraj, Abdel-Jawad, Madhat, Simin Liu, Macrossan, Michael, and Da Costa, Jc Diniz
12. Multiple subpial transections for medically refractory multifocal epilepsy
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Da Costa, Jc, Palmini, A., Maria Elisa Calcagnotto, Appel, Chc, and Portuguez, Mw
13. Safety and feasibility of intra-arterial autologous bone marrow mononuclear cells transplantation in acute ischemic stroke
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Freitas, Gr, Furtado-De-Mendonca, Ml, Bezerra, Dc, Silva, Sa, Falcao, Ch, Goncalez, Cs, Moreira, Rc, Haddad, Af, Tuche, Fa, Claudio Mesquita, Oliveira, Aa, Araujo, Md, Garicochea, B., Machado, Dc, Raupp, Ef, Andre, C., Dohmann, Hj, Borojevic, R., Da Costa, Jc, Mendez-Otero, R., Dohmann, H., and Friederich, M.
14. Neurosurgical pathology of 590 epilepsy patients in southern Brazil
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Barbosa-Coutinho, Lm, Hilbig, A., Ribeiro, M., Maria Elisa Calcagnotto, Da Costa, Jc, Palmini, A., Paglioli-Neto, E., and Paula, E.
15. Hypoglycemic effects of Cecropia pachystachya in normal and alloxan-induced diabetic rats.
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Aragão DMO, Guarize L, Lanini J, da Costa JC, Garcia RMG, and Scio E
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ETHNOPHARMACOLOGICAL RELEVANCE: Leaves of Cecropia pachystachya are described in the folk medicine as possessing antitusive, expectorant, antiasthmatic and hypoglycemic effects. AIM OF THE STUDY: To investigate the hypoglycemic and antioxidant effects of methanolic extract from the leaves of Cecropia pachystachya. The total amount of phenolic and flavonoids compounds was determined and the qualitative composition of the extract was analyzed. MATERIALS AND METHODS: The hypoglycemic effect of the extract was tested in normal, glucose loading and alloxan-induced diabetic rats. The antioxidant activity was assessed by DPPH free radical scavenging and reduction power assays. The total amount of phenolic and flavonoids compounds was determined by Folin-Denis and AlCl(3) reagent method, respectively. The qualitative composition of the extract was analyzed using a HPLC-DAD system. RESULTS: The glucose tolerance test showed that in diabetic rats, the extract caused a significant hypoglycemic effect with a blood glucose reduction of 68% after 12 h. The administration of the extract in alloxan-induced diabetic rats also produced a significant reduction in the blood glucose levels at all points being more pronounced at 90 min (reduction of 60%). After 120 min, no significant difference was observed between the blood levels of the rats treated with the extract and those treated with the standard drugs (metformin and glibenclamide). The extract also presented relevant antioxidant activity with IC(50)=3.1 µg/ml (DPPH assay) and EC(50)=10.8 µg/ml (reduction power). Results were compared with the reference antioxidants quercetin, rutin, and ascorbic acid. The content of flavonoids was 83 mg/g plant and that of phenolics was 326 mg/g plant. Chlorogenic acid and the C-glycosylated flavones, orientin and isoorientin, were identified in the extract. CONCLUSIONS: In conclusion, the findings showed that the folk medicinal plant Cecropia pachystachya possesses hypoglycemic and antioxidant effects which confirmed the traditional use of the plant in the treatment of diabetes. Chlorogenic acid and the C-glycosylated flavonoids may explain these activities. [ABSTRACT FROM AUTHOR]
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- 2010
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16. Physical Activity and Sedentary Behavior on Bone Health in Adults: A Systematic Review.
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da Costa JC, Barbosa CCL, Pelegrini A, Serassuelo Junior H, Gomez-Campos RA, Fernandes RA, and Ronque ERV
- Abstract
The objective was to analyze the longitudinal associations of physical activity (PA) and Sedentary behavior (SB) on bone health indicators (BHI) in healthy young adults. Articles were selected from five databases and 17 longitudinal studies were selected after meeting the inclusion and exclusion criteria. Among these, only one study assessed the association between childhood PA and adult BHI, the results showed no significant association between PA and BHI. Positive associations between adolescence and adulthood were observed between PA with bone mineral content and density and bone microarchitecture. In adulthood, the results were divergent; among the three studies located, two found a positive association. SB was negatively associated with bone mineral density and content from childhood to adulthood. However, from adolescence to adulthood, it was negatively associated with total tibia area but positively associated with trabecular thickness, cortical thickness, and cortical bone mineral. The evidence of the positive effect of PA and its intensities between adolescence and adulthood on BHI seems to be more consolidated, and it is still necessary to understand the role of intensities, volume of PA and SB in different periods of life with BHI in adulthood., Competing Interests: The authors declare that they have no conflict of interest., (Thieme. All rights reserved.)
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- 2024
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17. Development and Characterization of 3D-Printed PLA/Exfoliated Graphite Composites for Enhanced Electrochemical Performance in Energy Storage Applications.
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Dos Santos AL, de Souza FCR, Martins da Costa JC, Gonçalves DA, Passos RR, and Pocrifka LA
- Abstract
This research introduces a new way to create a composite material (PLA/EG) for 3D printing. It combines polylactic acid (PLA) with exfoliated graphite (EG) using a physical mixing method, followed by direct mixing in a single-screw extruder. Structural and vibrational analyses using X-ray diffraction and Fourier transform infrared spectroscopy confirmed the PLA/EG's formation (composite). The analysis also suggests physical adsorption as the primary interaction between the two materials. The exfoliated graphite acts as a barrier (thermal behavior), reducing heat transfer via TG. Electrochemical measurements reveal redox activity (cyclic voltammetry) with a specific capacitance of ~ 6 F g
-1 , low solution resistance, and negligible charge transfer resistance, indicating ion movement through a Warburg diffusion process. Additionally, in terms of complex behavior (electrochemical impedance spectroscopy), the PLA/EG's actual capacitance C'(ω) displayed a value greater than 1000 μF cm-2 , highlighting the composite's effectiveness in storing charge. These results demonstrate that PLA/EG composites hold significant promise as electrodes in electronic devices. The methodology used in this study not only provides a practical way to create functional composites but also opens doors for new applications in electronics and energy storage.- Published
- 2024
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18. Non-Cytotoxic Graphene Nanoplatelets Upregulate Cell Proliferation and Self-Renewal Genes of Mesenchymal Stem Cells.
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Nicoletti NF, Marinowic DR, Perondi D, Budelon Gonçalves JI, Piazza D, da Costa JC, and Falavigna A
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- Animals, Mice, Chlorocebus aethiops, Cell Self Renewal drug effects, Cell Self Renewal genetics, Vero Cells, Gene Expression Regulation drug effects, Nanoparticles chemistry, Cell Line, Nanostructures chemistry, Cell Proliferation drug effects, Mesenchymal Stem Cells drug effects, Mesenchymal Stem Cells metabolism, Mesenchymal Stem Cells cytology, Graphite chemistry, Graphite pharmacology
- Abstract
Graphene nanoplatelets (UGZ-1004) are emerging as a promising biomaterial in regenerative medicine. This study comprehensively evaluates UGZ-1004, focusing on its physical properties, cytotoxicity, intracellular interactions, and, notably, its effects on mesenchymal stem cells (MSCs). UGZ-1004 was characterized by lateral dimensions and layer counts consistent with ISO standards and demonstrated a high carbon purity of 0.08%. Cytotoxicity assessments revealed that UGZ-1004 is non-toxic to various cell lines, including 3T3 fibroblasts, VERO kidney epithelial cells, BV-2 microglia, and MSCs, in accordance with ISO 10993-5:2020/2023 guidelines. The study focused on MSCs and revealed that UGZ-1004 supports their gene expression alterations related to self-renewal and proliferation. MSCs exposed to UGZ-1004 maintained their characteristic surface markers. Importantly, UGZ-1004 promoted significant upregulation of genes crucial for cell cycle regulation and DNA repair, such as CDK1, CDK2, and MDM2. This gene expression profile suggests that UGZ-1004 can enhance MSC self-renewal capabilities, ensuring robust cellular function and longevity. Moreover, UGZ-1004 exposure led to the downregulation of genes associated with tumor development, including CCND1 and TFDP1, mitigating potential tumorigenic risks. These findings underscore the potential of UGZ-1004 to not only bolster MSC proliferation but also enhance their self-renewal processes, which are critical for effective regenerative therapies. The study highlights the need for continued research into the long-term impacts of graphene nanoplatelets and their application in MSC-based regenerative medicine.
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- 2024
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19. Influence of Different Diagnostic Criteria on Alzheimer Disease Clinical Research.
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Bieger A, Brum WS, Borelli WV, Therriault J, De Bastiani MA, Moreira AG, Benedet AL, Ferrari-Souza JP, Da Costa JC, Souza DO, Castilhos RM, Schumacher Schuh AF, Fagundes Chaves ML, Schöll M, Zetterberg H, Blennow K, Pascoal TA, Gauthier S, Rosa-Neto P, Schilling LP, and Zimmer ER
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- Humans, Aged, Female, Male, Aged, 80 and over, Cognitive Dysfunction diagnosis, Biomarkers, Practice Guidelines as Topic standards, Neuroimaging, Cohort Studies, Biomedical Research standards, Biomedical Research methods, Alzheimer Disease diagnosis
- Abstract
Background and Objectives: Updates in Alzheimer disease (AD) diagnostic guidelines by the National Institute on Aging-Alzheimer's Association (NIA-AA) and the International Working Group (IWG) over the past 11 years may affect clinical diagnoses. We assessed how these guidelines affect clinical AD diagnosis in a cohort of cognitively unimpaired (CU) and cognitively impaired (CI) individuals., Methods: We applied clinical and biomarker data in algorithms to classify individuals from the Alzheimer's Disease Neuroimaging Initiative (ADNI) cohort according to the following diagnostic guidelines for AD: 2011 NIA-AA, 2016 IWG-2, 2018 NIA-AA, and 2021 IWG-3, assigning the following generic diagnostic labels: (1) not AD (nAD), (2) increased risk of developing AD (irAD), and (3) AD. Diagnostic labels were compared according to their frequency, convergence across guidelines, biomarker profiles, and prognostic value. We also evaluated the diagnostic discordance among the criteria., Results: A total of 1,195 individuals (mean age 73.2 ± 7.2 years, mean education 16.1 ± 2.7, 44.0% female) presented different repartitions of diagnostic labels according to the 2011 NIA-AA (nAD = 37.8%, irAD = 23.0%, AD = 39.2%), 2016 IWG-2 (nAD = 37.7%, irAD = 28.7%, AD = 33.6%), 2018 NIA-AA (nAD = 40.7%, irAD = 9.3%, AD = 50.0%), and 2021 IWG-3 (nAD = 51.2%, irAD = 8.4%, AD = 48.3%) frameworks. Discordant diagnoses across all guidelines were found in 512 participants (42.8%) (138 [91.4%] occurring in only β-amyloid [CU 65.4%, CI 34.6%] and 191 [78.6%] in only tau-positive [CU 71.7%, CI 28.3%] individuals). Differences in predicting cognitive impairment between nAD and irAD groups were observed with the 2011 NIA-AA (hazard ratio [HR] 2.21, 95% CI 1.34-3.65, p = 0.002), 2016 IWG-2 (HR 2.81, 95% CI 1.59-4.96, p < 0.000), and 2021 IWG-3 (HR 3.61, 95% CI 2.09-6.23, p < 0.000), but not with 2018 NIA-AA (HR 1.69, 95% CI 0.87-3.28, p = 0.115)., Discussion: Over 42% of the studied population presented discordant diagnoses when using the different examined AD criteria, mostly in individuals with a single positive biomarker. Except for 2018 NIA-AA, all guidelines identified asymptomatic individuals at risk of cognitive impairment. Our findings highlight important differences between the guidelines, emphasizing the necessity for updated criteria with enhanced staging metrics, considering clinical, research, therapeutic, and trial design aspects.
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- 2024
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20. Physical fitness components are bone mineral density predictors in adulthood: cross-sectional study.
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da Costa JC, de Freitas MCM, Barbosa CCL, Guzmán AB, de Campos LFCC, Gómez-Campos R, Cossio-Bolaños M, and Ronque ERV
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- Humans, Male, Female, Cross-Sectional Studies, Young Adult, Adult, Adolescent, Muscle Strength physiology, Hand Strength physiology, Body Composition physiology, Bone Density physiology, Physical Fitness physiology, Absorptiometry, Photon
- Abstract
Background: Health-related physical fitness (HRPF) attributes are considered important markers beneficial to various health outcomes. However, the literature is divergent regarding HRPF and bone health in adulthood, especially due to the end of the second and beginning of the third decades of life when the peak bone mass period occurs., Objective: To analyze which HRPF variables are areal bone mineral density (aBMD) predictors in adult males and females., Methods: This study evaluated 137 healthy young adults aged 18-25 years (50% males). Dual-energy X-ray absorptiometry (DXA) was used to estimate fat mass and lean mass and aBMD, hand grip strength test, sit-ups test, flexibility test, lower limb muscle strength and 20-meter run were used to evaluate physical fitness. Multiple linear regression using the backward method was used to analyze bone mineral density predictors by sex., Results: HRPF indicators showed correlations from R = 0.28 in the right femoral neck aBMD to R = 0.61 in the upper limbs aBMD in males; in females, correlations from R = 0.27 in total body aBMD to R = 0.68 in the lower limbs aBMD were found. In males, body mass and HRPF indicators were aBMD predictors with HRPF indicators explaining variance from R²=0.214 in the lumbar spine to R²=0.497 in the upper limbs, and in females, with the exception of the lumbar spine, variance from R²=0.237 in the right femoral neck aBMD to R²=0.442 in the lower limbs aBMD was found., Conclusion: Health-related physical fitness components were able to predict aBMD in different anatomical regions in young adults, especially muscle strength and cardiorespiratory fitness indicators for males, while only lean mass and fat mass for females., (© 2024. The Author(s).)
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- 2024
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21. Case Report: Molecular Analyses of Cell-Cycle-Related Genes in Cortical Brain Tissue of a Patient with Rasmussen Encephalitis.
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Gonçalves JIB, de Castro VR, Martins WA, Xavier FAC, Da Costa JC, Neto EP, Palmini A, and Marinowic DR
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- Humans, Male, Cell Cycle Proteins genetics, Cell Cycle Proteins metabolism, Brain pathology, Brain metabolism, Brain-Derived Neurotrophic Factor genetics, Brain-Derived Neurotrophic Factor metabolism, Cerebral Cortex pathology, Cerebral Cortex metabolism, Female, Tumor Necrosis Factor-alpha metabolism, Tumor Necrosis Factor-alpha genetics, Cell Cycle genetics, Encephalitis genetics, Encephalitis pathology, Encephalitis metabolism
- Abstract
Rasmussen's encephalitis (RE) stands as a rare neurological disorder marked by progressive cerebral hemiatrophy and epilepsy resistant to medical treatment. Despite extensive study, the primary cause of RE remains elusive, while its histopathological features encompass cortical inflammation, neuronal degeneration, and gliosis. The underlying molecular mechanisms driving disease progression remain largely unexplored. In this case study, we present a patient with RE who underwent hemispherotomy and has remained seizure-free for over six months, experiencing gradual motor improvement. Furthermore, we conducted molecular analysis on the excised brain tissue, unveiling a decrease in the expression of cell-cycle-associated genes coupled with elevated levels of BDNF and TNF-α proteins. These findings suggest the potential involvement of cell cycle regulators in the progression of RE.
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- 2024
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22. Are COVID-19 Polymorphisms in ACE and ACE2 Prognosis Predictors?
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Guarienti FA, Xavier FAC, Ferraz MD, Wagner F, Marinowic DR, da Costa JC, and Machado DC
- Abstract
Regardless of the containment of the SARS-CoV-2 pandemic, it remains paramount to comprehensively understand its underlying mechanisms to mitigate potential future health and economic impacts, comparable to those experienced throughout the course of the pandemic. The angiotensin-converting enzyme 2 (ACE2) provides anchorage for SARS-CoV-2 binding, thus implicating that ACE and ACE2 might contribute to the variability in infection severity. This study aimed to elucidate predisposing factors influencing the disease course among people infected by SARS-CoV-2, focusing on angiotensin-converting enzyme (ACE) and ACE2 polymorphisms. Notably, despite similar demographics and comorbidities, COVID-19 patients exhibit substantial differences in prognosis. Genetic polymorphisms in ACE and ACE2 have been implicated in disease progression, prompting our investigation into their role in COVID-19 evolution. Using next-generation sequencing (NGS), we analyzed ACE and ACE2 genes in a sample group comprising six subjects infected by SARS-CoV-2. Our findings revealed a correlation between specific polymorphisms and COVID-19 outcomes. Specifically, ACE and ACE2 intronic deletions were observed in all deceased patients, suggesting a potential association with mortality. These results highlight the significance of genetic factors in shaping the clinical course of COVID-19, emphasizing the importance of further research into the impact of genetic variations on COVID-19 severity.
- Published
- 2024
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23. Nutritional and Inflammatory Markers Associated with SARS-CoV-2 Infection in the Elderly.
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Budelon Gonçalves JI, Lermen FM, Gonçalves JB, Zanirati G, Machado DC, Marques HM, Erwig HS, Becker BM, Wagner F, Boff MO, Rocha MG, Da Costa JC, and Marinowic ED
- Subjects
- Humans, Aged, Male, Female, Aged, 80 and over, Severity of Illness Index, Inflammation blood, Nutritional Status, Brazil epidemiology, Cohort Studies, Body Mass Index, Overweight blood, COVID-19 blood, Biomarkers blood, SARS-CoV-2
- Abstract
The COVID-19 pandemic, caused by the SARS-CoV-2 virus, has posed unprecedented challenges to global health systems, particularly among vulnerable populations such as the elderly. Understanding the interplay between anthropometric markers, molecular profiles, and disease severity is crucial for effective clinical management and intervention strategies. We conducted a cohort study comprising 43 elderly COVID-19 patients admitted to São Lucas Hospital, PUCRS, Brazil. Anthropometric measurements, including calf circumference (CC) and abdominal circumference (AC), were assessed alongside molecular analyses of peripheral blood samples obtained within 48 h of hospital admission. Sociodemographic data were collected from electronic medical records for comprehensive analysis. Our findings revealed a possible relationship between overweight status, increased abdominal adiposity, and prolonged hospitalization duration, alongside heightened disease severity. We also found no significant correlations between BMI, vitamin D levels, and clinical outcomes. Elevated oxygen requirements were observed in both normal and overweight individuals, with the latter necessitating prolonged oxygen therapy. Molecular analyses revealed changes in the inflammatory profile regarding the outcome of the patients. Our study highlights the critical importance of both anthropometric and molecular markers in predicting disease severity and clinical outcomes in elderly individuals with COVID-19.
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- 2024
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24. Extracellular Vesicles: The Next Generation of Biomarkers and Treatment for Central Nervous System Diseases.
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Zanirati G, Dos Santos PG, Alcará AM, Bruzzo F, Ghilardi IM, Wietholter V, Xavier FAC, Gonçalves JIB, Marinowic D, Shetty AK, and da Costa JC
- Subjects
- Humans, Animals, MicroRNAs genetics, MicroRNAs metabolism, Mesenchymal Stem Cells metabolism, Neurodegenerative Diseases therapy, Neurodegenerative Diseases metabolism, Neurodegenerative Diseases diagnosis, Extracellular Vesicles metabolism, Biomarkers, Central Nervous System Diseases metabolism, Central Nervous System Diseases therapy, Central Nervous System Diseases diagnosis
- Abstract
It has been widely established that the characterization of extracellular vesicles (EVs), particularly small EVs (sEVs), shed by different cell types into biofluids, helps to identify biomarkers and therapeutic targets in neurological and neurodegenerative diseases. Recent studies are also exploring the efficacy of mesenchymal stem cell-derived extracellular vesicles naturally enriched with therapeutic microRNAs and proteins for treating various diseases. In addition, EVs released by various neural cells play a crucial function in the modulation of signal transmission in the brain in physiological conditions. However, in pathological conditions, such EVs can facilitate the spread of pathological proteins from one brain region to the other. On the other hand, the analysis of EVs in biofluids can identify sensitive biomarkers for diagnosis, prognosis, and disease progression. This review discusses the potential therapeutic use of stem cell-derived EVs in several central nervous system diseases. It lists their differences and similarities and confers various studies exploring EVs as biomarkers. Further advances in EV research in the coming years will likely lead to the routine use of EVs in therapeutic settings.
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- 2024
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25. Zinc-Modified Titanate Nanotubes as Radiosensitizers for Glioblastoma: Enhancing Radiotherapy Efficacy and Monte Carlo Simulations.
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Diz FM, Monteiro WF, Silveira IS, Ruano D, Zotti ER, Weimer RD, Melo MN, Schossler Lopes JG, Scheffel TB, Caldas LVE, da Costa JC, Morrone FB, and Ligabue RA
- Abstract
Radiotherapy (RT) is the established noninvasive treatment for glioblastoma (GBM), a highly aggressive malignancy. However, its effectiveness in improving patient survival remains limited due to the radioresistant nature of GBM. Metal-based nanostructures have emerged as promising strategies to enhance RT efficacy. Among them, titanate nanotubes (TNTs) have gained significant attention due to their biocompatibility and cost-effectiveness. This study aimed to synthesize zinc-modified TNTs (ZnTNT) from sodium TNTs (NaTNT), in addition to characterizing the formed nanostructures and evaluating their radiosensitization effects in GBM cells (U87 and U251). Hydrothermal synthesis was employed to fabricate the TNTs, which were characterized using various techniques, including transmission electron microscopy (TEM), energy-dispersive spectroscopy, scanning-transmission mode, Fourier-transform infrared spectroscopy, ICP-MS (inductively coupled plasma mass spectrometry), X-ray photoelectron spectroscopy, and zeta potential analysis. Cytotoxicity was evaluated in healthy (Vero) and GBM (U87 and U251) cells by the MTT assay, while the internalization of TNTs was observed through TEM imaging and ICP-MS. The radiosensitivity of ZnTNT and NaTNT combined with 5 Gy was evaluated using clonogenic assays. Monte Carlo simulations using the MCNP6.2 code were performed to determine the deposited dose in the culture medium for RT scenarios involving TNT clusters and cells. The results demonstrated differences in the dose deposition values between the scenarios with and without TNTs. The study revealed that ZnTNT interfered with clonogenic integrity, suggesting its potential as a powerful tool for GBM treatment., Competing Interests: The authors declare no competing financial interest., (© 2024 The Authors. Published by American Chemical Society.)
- Published
- 2024
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26. Evaluation of the effects of the Zika Virus-Immunoglobulin G + complex on murine microglial cells.
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da Silva Siqueira L, Rodrigues FVF, Zanatta Â, Gonçalves JIB, Ghilardi IM, Alcará AM, Becker NB, Pinzetta G, Zanirati G, Becker BMA, Erwig HS, da Costa JC, and Marinowic DR
- Abstract
After the Zika virus (ZIKV) epidemic in Brazil, ZIKV infections were linked to damage to the central nervous system (CNS) and congenital anomalies. Due to the virus's ability to cross the placenta and reach brain tissue, its effects become severe, leading to Congenital Zika Syndrome (CZS) and resulting in neuroinflammation, microglial activation, and secretion of neurotoxic factors. The presence of ZIKV triggers an inadequate fetal immune response, as the fetus only has the protection of maternal antibodies of the Immunoglobulin G (IgG) class, which are the only antibodies capable of crossing the placenta. Because of limited understanding regarding the long term consequences of ZIKV infection and the involvement of maternal antibodies, this study sought to assess the impact of the ZIKV + IgG⁺complex on murine microglial cells. The cells were exposed to ZIKV, IgG antibodies, and the ZIKV + IgG⁺complex for 24 and 72 h. Treatment-induced cytotoxic effects were evaluated using the cell viability assay, oxidative stress, and mitochondrial membrane potential. The findings indicated that IgG antibodies exhibit cytotoxic effects on microglia, whether alone or in the presence of ZIKV, leading to compromised cell viability, disrupted mitochondrial membrane potential, and heightened oxidative damage. Our conclusion is that IgG antibodies exert detrimental effects on microglia, triggering their activation and potentially disrupting the creation of a neurotoxic environment. Moreover, the presence of antibodies may correlate with an elevated risk of ZIKV-induced neuroinflammation, contributing to long-term CNS damage., (© 2024. The Author(s) under exclusive licence to The Journal of NeuroVirology, Inc.)
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- 2024
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27. Factor Associated with Adherence to the Protein and Fat Counting Strategy by Adults with Type 1 Diabetes Mellitus.
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Uliana GC, da Costa JC, Quaresma AR, da Fonseca AA, Ohaze KB, Alves LSC, and Gomes DL
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- Humans, Male, Adult, Female, Cross-Sectional Studies, Patient Compliance, Middle Aged, Dietary Fats administration & dosage, Glycemic Control methods, Young Adult, Surveys and Questionnaires, Blood Glucose metabolism, Diabetes Mellitus, Type 1 blood, Dietary Proteins administration & dosage, Glycated Hemoglobin metabolism, Glycated Hemoglobin analysis
- Abstract
Carbohydrate counting is one of the dietary strategies used for the management of type 1 diabetes (T1DM), and counting proteins and fats allows individuals to achieve better glycemic and metabolic control, reducing glycemic variability and long-term complications. The aim of this paper is to analyze the factors associated with adherence to the protein- and fat-counting strategy in adults with T1DM. This cross-sectional study was conducted from November 2021 to June 2022 through an online questionnaire. We applied Pearson's Chi-square test with adjusted residual analysis and a binomial logistic regression test using SPSS software, version 24.0, considering p < 0.05 as indicative of statistical significance. There was an association between performing protein and lipid counting and having a higher education level, income exceeding three minimum wages, and having adequate glycated hemoglobin. Performing protein and lipid counting increased the chances of having adequate HbA1c by 4.3 times. Protein and lipid counting was a predictor of having adequate HbA1c. The results suggest that considering the practice of counting proteins and fats is important as a strategy to optimize glycemic control.
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- 2024
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28. Correlation between low handgrip strength and metabolic syndrome in older adults: a systematic review.
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d'Avila JC, Moreira El Nabbout TG, Georges Moreira El Nabbout H, Silva ADS, Barbosa Ramos Junior AC, Fonseca ERD, Santana Carlos A, and de Azeredo Siqueira R
- Subjects
- Humans, Aged, Male, Female, Muscle Weakness physiopathology, Risk Factors, Insulin Resistance physiology, Metabolic Syndrome physiopathology, Hand Strength physiology
- Abstract
Muscle weakness has been associated to insulin resistance and metabolic syndrome in the general population. However, it is still unclear whether this association is maintained in older adults. This study investigated correlations between low handgrip strength (HGS) and metabolic syndrome, or some of its components, in older adults through a systematic review of the literature. Searches were conducted in the Virtual Health Library Regional Portal, Scopus, Cochrane, Embase, MEDLINE/ PubMed, SciELO, and Web of Science databases for relevant studiesinvestigating muscle weakness (measured by hand dynamometer) and metabolic syndrome or its components in older adult populations, published up to September 2023. From the 2050 references initially identified, 20 studies, comprising a total of 31,264 older adults of both genders, completely met the inclusion/exclusion criteria. Eighteen studies showed that lower HGS was associated with metabolic syndrome or some of its risk factors, such as abdominal obesity, hyperglycemia, insulin resistance, dyslipidemia, or high blood pressure. Two studies found that older men with high blood pressure had increased HGS. Most studies included in this systematic review revealed a significant correlation between reduced HGS and metabolic syndrome or some of its components, especially abdominal obesity and insulin resistance. We conclude that below-average HGS can be associated with metabolic syndrome in older adults.
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- 2024
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29. Induced Pluripotent Stem Cells and Organoids in Advancing Neuropathology Research and Therapies.
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Pazzin DB, Previato TTR, Budelon Gonçalves JI, Zanirati G, Xavier FAC, da Costa JC, and Marinowic DR
- Subjects
- Humans, Animals, Neuropathology methods, Regenerative Medicine methods, Neurodegenerative Diseases therapy, Neurodegenerative Diseases pathology, Cell Differentiation, Organoids pathology, Induced Pluripotent Stem Cells cytology
- Abstract
This review delves into the groundbreaking impact of induced pluripotent stem cells (iPSCs) and three-dimensional organoid models in propelling forward neuropathology research. With a focus on neurodegenerative diseases, neuromotor disorders, and related conditions, iPSCs provide a platform for personalized disease modeling, holding significant potential for regenerative therapy and drug discovery. The adaptability of iPSCs, along with associated methodologies, enables the generation of various types of neural cell differentiations and their integration into three-dimensional organoid models, effectively replicating complex tissue structures in vitro. Key advancements in organoid and iPSC generation protocols, alongside the careful selection of donor cell types, are emphasized as critical steps in harnessing these technologies to mitigate tumorigenic risks and other hurdles. Encouragingly, iPSCs show promising outcomes in regenerative therapies, as evidenced by their successful application in animal models.
- Published
- 2024
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30. Effects of climate change and mixtures of pesticides on the Amazonian fish Colossoma macropomum.
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de Souza SS, Bruce KHR, da Costa JC, Pereira D, da Silva GS, and Val AL
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- Animals, Climate Change, Antioxidants metabolism, Malathion, Pesticides toxicity, Characiformes metabolism
- Abstract
Several studies highlighted the complexity of mixing pesticides present in Amazonian aquatic environments today. There is evidence that indicates that ongoing climate change can alter the pattern of pesticide use, increasing the concentration and frequency of pesticide applications. It is known that the combination of thermal and chemical stress can induce interactive effects in aquatic biota, which accentuates cell and molecular damage. However, considering that the effects of climate change go beyond the increase in temperature the objective of this study was to evaluate the effect of climate change scenarios proposed by 6 th IPCC report and a mixture of pesticides on the tambaqui (Colossoma macropomum). The hypothesis of this study is that the negative effects will be accentuated by the combination of an extreme climate changes scenario and a mixture of pesticides. To test the hypothesis, juvenile tambaqui were exposed to a combination of four pesticides (chlorpyrifos, malathion, carbendazim and atrazine) in two scenarios, one that simulates current environmental conditions and another that predicted the environmental scenario for the year 2100. Fish were subjected to the experimental conditions for 96 h. At the end of the experiment, samples of blood, gills, liver, brain, and muscle were obtained for hematological, genotoxic, biochemical, and histopathological analyses. The results demonstrate that environmentally realistic concentrations of pesticides, when mixed, can alter the biochemical responses of tambaqui. The extreme scenario promotes hematological adjustments, but impairs branchial antioxidant enzymes. There is an interaction between the mixture of pesticides and the extreme scenario, accentuating liver tissue damage, which demonstrates that even increased activity of antioxidant and biotransformation enzymes were not sufficient to prevent liver damage., Competing Interests: Declaration of competing interest The authors have no conflicts of interest to declare., (Copyright © 2024. Published by Elsevier B.V.)
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- 2024
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31. In vivo exposure to high temperature compromises quality of the sperm in Colossoma macropomum.
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da Silva Castro J, Santos FBP, da Costa JC, Heinrichs-Caldas W, and Val AL
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- Male, Animals, Temperature, Sperm Motility, Spermatozoa, Semen, Characiformes
- Abstract
Increases in temperature can affect the reproduction of fish by decreasing the quality of gametes for fertilization. Therefore, this study aimed to evaluate the in vivo effect of temperature on the production and sperm quality of Colossoma macropomum, which is an economically relevant species for Brazil, and other countries. Broodstock were exposed for 10 days at temperatures of 29 °C (n = 4) and 35 °C (n = 4). After exposure, semen was collected and sperm quality was evaluated for kinetic, biochemical, morphological parameters, membrane integrity, and oxygen consumption. The sperm quality of males of specimens of C. macropomum exposed in vivo to the higher temperature was compromised and showed a difference in all the analyses (P < 0.05). Sperm showed decreased motility (51.9 ± 11.6 s) compared to the control (61.3 ± 5.5 s); curvilinear (CLV), straight line (SLV), and average path (APV) velocities decreased, and straightness (STR) and beat cross frequency (BCF) increased; oxygen consumption decreased (32.8 ± 3.4 pmol. (s.ml)
-1 , picomol per second per ml) compared to the control (46.6 ± 3.5 pmol. (s.ml)-1 ); and the activity of catalase (CAT) and glutathione S-transferase (GST) enzymes increased and decreased, respectively, in sperm from fish exposed to high temperatures. Furthermore, an increase in non-viable sperm with damaged membranes and sperm with altered morphology was observed. The observed alterations indicate that C. macropomum confined in environments with high temperatures possibly produces more sperm that are unviable for the fertilization process., Competing Interests: Declaration of Competing Interest The authors declare that there is no conflict of interest., (Copyright © 2024 Elsevier B.V. All rights reserved.)- Published
- 2024
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32. Influence of Zika virus on the cytotoxicity, cell adhesion, apoptosis and inflammatory markers of glioblastoma cells.
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Marinowic DR, Zanirati GG, Azevedo PN, Zanatta Â, Plentz I, Alcará AM, Morrone FB, Scheffel TB, Cappellari AR, Roehe PM, Muterle Varela AP, Machado DC, Spillari Viola F, and Da Costa JC
- Abstract
Glioblastoma (GBM) is one of the most common types of brain tumor in adults. Despite the availability of treatments for this disease, GBM remains one of the most lethal and difficult types of tumors to treat, and thus, a majority of patients die within 2 years of diagnosis. Infection with Zika virus (ZIKV) inhibits cell proliferation and induces apoptosis, particularly in developing neuronal cells, and thus could potentially be considered an alternative for GBM treatment. In the present study, two GBM cell lines (U-138 and U-251) were infected with ZIKV at different multiplicities of infection (0.1, 0.01 and 0.001), and cell viability, migration, adhesion, induction of apoptosis, interleukin levels and CD14/CD73 cell surface marker expression were analyzed. The present study demonstrated that ZIKV infection promoted loss of cell viability and increased apoptosis in U-138 cells, as measured by MTT and triplex assay, respectively. Changes in cell migration, as determined by wound healing assay, were not observed; however, the GBM cell lines exhibited an increase in cell adhesion when compared with non-tumoral cells (Vero). The Luminex immunoassay showed a significant increase in the expression levels of IL-4 specifically in U-251 cells (MOI 0.001) following exposure to ZIKV. There was no significant change in the expression levels of IFN-γ upon ZIKV infection in the cell lines tested. Furthermore, a marked increase in the percentage of cells expressing the CD14 surface marker was observed in both GBM cell lines compared with in Vero cells; and significantly increased CD73 expression was observed particularly in U-251 cells, when compared with uninfected cells. These findings indicate that ZIKV infection could lead to reduced cell viability, elevated CD73 expression, improved cellular adherence, and higher rates of apoptosis in glioblastoma cells. Further studies are required to explore the potential use of ZIKV in the treatment of GBM., Competing Interests: JCDC is a researcher of Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq; Federal District, Brazil), which provided funding for the present study. The other authors declare that they have no competing interests., (Copyright: © 2024 Marinowic et al.)
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- 2024
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33. The cortical thickness of tricenarian cocaine users assembles features of an octogenarian brain.
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Rothmann LM, Tondo LP, Borelli WV, Esper NB, Portolan ET, Franco AR, Portuguez MW, Ferreira PE, Bittencourt AML, Soder RB, Viola TW, da Costa JC, and Grassi-Oliveira R
- Subjects
- Adult, Aged, 80 and over, Humans, Aged, Cross-Sectional Studies, Brain diagnostic imaging, Head, Octogenarians, Cocaine
- Abstract
It has been suggested that substance use disorders could lead to accelerated biological aging, but only a few neuroimaging studies have investigated this hypothesis so far. In this cross-sectional study, structural neuroimaging was performed to measure cortical thickness (CT) in tricenarian adults with cocaine use disorder (CUD, n
1 = 30) and their age-paired controls (YC, n1 = 30), and compare it with octogenarian elder controls (EC, n1 = 20). We found that CT in the right fusiform gyrus was similar between CUD and EC, thinner than the expected values of YC. We also found that regarding CT of the right inferior temporal gyrus, right inferior parietal cortex, and left superior parietal cortex, the CUD group exhibited parameters that fell in between EC and YC groups. Finally, CT of the right pars triangularis bordering with orbitofrontal gyrus, right superior temporal gyrus, and right precentral gyrus were reduced in CUD when contrasted with YC, but those areas were unrelated to CT of EC. Despite the 50-year age gap between our age groups, CT of tricenarian cocaine users assembles features of an octogenarian brain, reinforcing the accelerated aging hypothesis in CUD., (© 2023 Wiley Periodicals LLC.)- Published
- 2024
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34. Exploring CD39 and CD73 Expression as Potential Biomarkers in Prostate Cancer.
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Gardani CFF, Pedrazza EL, Paz VS, Zanirati GG, da Costa JC, Andrejew R, Ulrich H, Scholl JN, Figueiró F, Rockenbach L, and Morrone FB
- Abstract
Prostate cancer (PC) is the most diagnosed tumor in males and ranks as the second leading cause of male mortality in the western world. The CD39 and CD73 enzymes play a crucial role in cancer regulation by degrading nucleotides and forming nucleosides. This study aimed to investigate the expression of the CD39 and CD73 enzymes as potential therapeutic targets for PC. The initial part of this study retrospectively analyzed tissue samples from 23 PC patients. Using the TissueFAXS
TM cytometry platform, we found significantly higher levels of CD39-labeling its intensity compared to CD73. Additionally, we observed a correlation between the Gleason score and the intensity of CD39 expression. In the prospective arm, blood samples were collected from 25 patients at the time of diagnosis and after six months of treatment to determine the expression of CD39 and CD73 in the serum extracellular vesicles (EVs) and to analyze nucleotide hydrolysis. Notably, the expression of CD39 in the EVs was significantly increased compared to the CD73 and/or combined CD39/CD73 expression levels at initial collection. Furthermore, our results demonstrated positive correlations between ADP hydrolysis and the transurethral resection and Gleason score. Understanding the role of ectonucleotidases is crucial for identifying new biomarkers in PC.- Published
- 2023
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35. WNT pathway in focal cortical dysplasia compared to perilesional nonlesional tissue in refractory epilepsies.
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Marinowic DR, Zanirati GG, Xavier FAC, Varella FJ, Azevedo SPDC, Ghilardi IM, Pereira-Neto NG, Koff MAE, Paglioli E, Palmini A, Abreu JG, Machado DC, and da Costa JC
- Subjects
- Child, Wnt Signaling Pathway genetics, Malformations of Cortical Development, Group I, beta Catenin, Epilepsy, Humans, Seizures, Focal Cortical Dysplasia, Drug Resistant Epilepsy genetics, Drug Resistant Epilepsy surgery
- Abstract
Background: Focal cortical dysplasia (FCD) is a malformation of cortical development that causes medical refractory seizures, and one of the main treatments may be surgical resection of the affected area of the brain. People affected by FCD may present with seizures of variable severity since childhood. Despite many medical treatments available, only surgery can offer cure. The pathophysiology of the disease is not yet understood; however, it is known that several gene alterations may play a role. The WNT/β-catenin pathway is closely related to the control and balance of cell proliferation and differentiation in the central nervous system. The aim of this study was to explore genes related to the WNT/β-catenin pathway in lesional and perilesional brain tissue in patients with FCD type II., Methods: Dysplastic and perilesional tissue from the primary dysplastic lesion of patients with FCD type IIa were obtained from two patients who underwent surgical treatment. The analysis of the relative expression of genes was performed by a qRT-PCR array (super array) containing 84 genes related to the WNT pathway., Results: Our results suggest the existence of molecular alteration in some genes of the WNT pathway in tissue with dysplastic lesions and of perilesional tissue. We call this tissue of normal-appearing adjacent cortex (NAAC). Of all genes analyzed, a large number of genes show similar behavior between injured, perilesional and control tissues. However, some genes have similar characteristics between the perilesional and lesional tissue and are different from the control brain tissue, presenting the perilesional tissue as a molecularly altered material., Conclusion: Our results suggest that the perilesional area after surgical resection of tissue with cortical dysplasia presents molecular changes that may play a role in the recurrence of seizures in these patients. The perilesional tissue should receive expanded attention beyond the somatic mutations described and associated with FCD, such as mTOR, for example, to new signaling pathways that may play a crucial role in seizure recurrence., (© 2023. BioMed Central Ltd., part of Springer Nature.)
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- 2023
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36. Characteristics of carbohydrate counting practice associated with adequacy of glycated hemoglobin in adults with type 1 diabetes mellitus in Brazil.
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Uliana GC, Camara LN, Paracampo CCP, da Costa JC, and Gomes DL
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- Adult, Humans, Glycated Hemoglobin, Brazil epidemiology, Cross-Sectional Studies, Diabetes Mellitus, Type 1, Nutrition Therapy
- Abstract
Background: The Carbohydrate Counting (CC) is directly associated with achieving glycemic control by people with Type 1 Diabetes Mellitus (T1DM). Therefore, this study aims to analyze characteristics of the CC practice associated with the adequacy of glycated hemoglobin (HbA1c) in adults with T1DM in Brazil., Methods: The study was cross-sectional, carried out using an online form with questions about knowledge of CC, clinical, anthropometric, sociodemographic data, follow-up with health professionals and understanding of the concepts of CC. Pearson's chi-square test and binomial logistic regression analysis (p<0.05) were applied., Results: 173 adults participated, of which 57.2% had increased HbA1c (≥7%). Having the diabetes duration <10 years (p=0.006), performing the CC at lunch (p=0.040) and dinner (p=0.018), using specific applications to perform the CC (p=0.001), having learned to perform CC with a nutritionist (p=0.037) and knowing how to correctly define the concepts of food bolus (p=0.001), correction bolus (p<0.001) and insulin/carbohydrate ratio (p<0.001) was associated with having adequate HbA1c (<7%). Participants who were undergoing CC practice were 3.273 times more likely to have adequate HbA1c and participants with diabetes duration <10 years were 2.686 times more likely to have adequate HbA1c., Conclusion: It was concluded that variables transversal to CC favor adequate HbA1c values in adults with T1DM and that practicing CC and having a diabetes duration of less than 10 years are predictive factors of having adequate HbA1c., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Uliana, Camara, Paracampo, da Costa and Gomes.)
- Published
- 2023
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37. Clinical validation and diagnostic accuracy of 99m Tc-EDDA/HYNIC-TOC compared to 111 In-DTPA-octreotide in patients with neuroendocrine tumours: the LACOG 0214 study.
- Author
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Moriguchi-Jeckel CM, Madke RR, Radaelli G, Viana A, Nabinger P, Fernandes B, Gössling G, Berdichevski EH, Vilas E, Giacomazzi J, Rocha MS, Borges JA, Hoffmann E, Greggio S, Venturin GT, Barrios CH, Zaffaroni F, Werutsky G, and da Costa JC
- Abstract
99m Tc-EDDA/HYNIC-TOC is an easily available and cheaper radionuclide that could be used for somatostatin-receptor-based imaging of neuroendocrine tumours (NETs). We aimed to evaluate the diagnostic performance of99m Tc-EDDA/HYNIC-TOC compared to111 In-DTPA-octreotide in patients (pts) with NETs. We performed a prospective diagnostic study including pts with biopsy-confirmed NET and at least one visible lesion at conventional imaging. Two independent nuclear medicine physicians evaluated pts who underwent99m Tc and111 In scans and images. The primary outcome was comparative diagnostic accuracy of99m Tc and111 In. Secondary outcomes include safety. Nine pts were included and performed 14 paired scans. Overall, 126 lesions were identified.99m Tc demonstrated superior sensitivity both when all images were analysed (93.7, 95% CI 88.1% - 96.8% versus 74.8%, 95% CI 66.6 - 81.6%, p < 0.001) and when liver-specific images were analysed (97.8%, 95% CI 92.7% - 99.5% versus 85.1%, 95% CI 76.6% - 91.0%, p < 0.001).99m Tc was also associated with a lower negative likelihood ratio (LR) (0.002, 95% CI 0.009 - 0.1 versus 0.19, 95% CI 0.12 - 0.42, p = 0.009) when evaluating hepatic lesions. Adverse events happened in 3 pts after111 In and in 2 pts after99m Tc, all grade 1. The99m Tc demonstrated a higher sensitivity overall and a better negative LR in liver-specific images compared to111 In in pts with NETs. Our findings suggest that99m Tc is an alternative to111 In and is especially useful in ruling out liver metastases. NCT02691078., Competing Interests: Dr Moriguchi-Jeckel and Dr Werutsky report grants from Financiadora de Estudos e Projetos (FINEP) for the conduct of the study. Drs R. Madke, Viana, Nabinger, and Fernandes report employment relationships with the RPH Group. Dr. Werutsky reports personal fees from AstraZeneca, Bayer, Beigene, Daiichi Sankyo, Genentech/Roche, GSK, Lilly, MSD, Novartis, Pfizer, Sanofi, and Seattle Genetics outside the submitted work. The other authors declare that they have no conflict of interest., (© the authors; licensee ecancermedicalscience.)- Published
- 2023
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38. Tailored Approach for Persistent Olfactory Dysfunction After SARS-CoV-2 Infection: A Pilot Study.
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Sousa FA, Machado AS, da Costa JC, Silva AC, Pinto AN, Coutinho MB, Meireles L, and Sousa CAE
- Subjects
- Humans, Pilot Projects, SARS-CoV-2, Smell, Glucocorticoids, COVID-19 complications, COVID-19 therapy, Olfaction Disorders diagnosis, Olfaction Disorders etiology, Olfaction Disorders therapy
- Abstract
Objective: So far, no original studies explored non-randomized, standardized protocols for COVID-19 associated olfactory dysfunction. The main objective was to determine the efficacy of a new protocol for post-COVID olfactopathy while assessing the benefit of adding adjuvant therapies to olfactory training., Methods: Patients suffering from long-lasting post-COVID-19 olfactory dysfunction were evaluated. A non-randomized protocol based on individual nasal endoscopy findings and patient's preferences was applied. Patients were assigned for olfactory training alone or olfactory training + adjuvant therapy. Participants performed olfactory objective and subjective evaluations at first consultation and 3 months after treatment, and results were compared., Results: A total of 47 patients were enrolled. All groups showed significant improvement in olfactory thresholds at 3-month follow-up suggesting protocol effectiveness (olfactory training group alone showed a mean threshold difference of 2.9, P < .001; Olfactory training + Topical Corticosteroid showed a mean threshold difference of 4, P = .006; Olfactory training + Topical Corticosteroid + Vitamin B complex showed a mean threshold difference of 4.4, P = .006; Olfactory training + Intranasal Vitamin A and E showed a mean threshold difference of 4.4, P < .001). Olfactory training alone showed lower mean olfactory threshold improvement, when compared to patients undergoing olfactory training + adjuvant therapy (olfactory training alone mean improvement 2.9 ± 2.3 vs olfactory training + adjuvants mean improvement 4.3 ± 2.458, P = .03)., Conclusions: This is one of the first studies to demonstrate results in the treatment of post-COVID-19 persistent olfactory impairment. A customized approach based on endoscopy findings and patient's preferences may be a valid option for the management of persistent post-COVID-19 olfactory disorder. Adjuvant therapy could be considered in addition to olfactory training, but further studies are needed in order to confirm their effectiveness in this setting., Level of Evidence: 2c (outcomes research).
- Published
- 2023
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39. Maturity-associated variation in the body size, physical fitness, technical efficiency, and network-based centrality measures in young soccer players.
- Author
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Borges PH, da Costa JC, Ramos-Silva LF, Menegassi VM, Praça GM, Moura FA, and Ronque ERV
- Subjects
- Humans, Male, Exercise Test methods, Physical Fitness, Body Size, Soccer, Athletic Performance, Running
- Abstract
This study aimed to observe the relationships between the maturity status on the network-based centrality measures of young athletes in small-sided soccer games (SSG). The study included 81 male players (14.4 ± 1.1 years). Measurements included height, sitting height, body mass, and bone age (TW3 method). The applied protocols were the following: Countermovement Jump (CMJ), Yo-Yo Intermittent Recovery Test Level 1 (YYIRT1), Repeated Sprints Ability (RSA), observational analysis of techniques, and interactions performed by players in SSG. The relationship between the set of evaluated variables within each maturity status was obtained from the correlational analysis of networks (P < 0.05). The maturity status explained a significant portion of the variance in body mass (η
2 = 0.37), height (η2 = 0.30), sitting height (η2 = 0.30), and performance on the YYIRT1 (η2 = 0.08), CMJ (η2 = 0.14), and RSA (η2 = 0.13). No effect of maturity status on network-based centrality measures of young athletes was identified (P > 0.05). For the late maturity group, there was a correlation between the degree of centrality and physical growth indicators (rmean = 0.88). For players with maturation "on time", physical growth indicators relate to the degree of prestige (rmean = 0.36). It is concluded that body size and bone age impact how late and on-time maturity groups interact within the match., (© 2023. The Author(s).)- Published
- 2023
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40. Interrelationship between muscle fitness in childhood and bone mineral density in adulthood: mediation analysis of muscle fitness in adulthood.
- Author
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Barbosa CCL, da Costa JC, Romanzini CLP, Batista MB, Blasquez-Shigaki G, Fernandes RA, Martinho DV, Oliveira T, Ribeiro LP, Coelho-E-Silva MJ, and Ronque ERV
- Subjects
- Child, Female, Humans, Male, Absorptiometry, Photon, Hand Strength physiology, Mediation Analysis, Young Adult, Lumbar Vertebrae diagnostic imaging, Lumbar Vertebrae physiology, Exercise Test, Upper Extremity diagnostic imaging, Upper Extremity physiology, Bone Density physiology, Muscle Strength physiology, Physical Fitness physiology
- Abstract
Background: This study was aimed to examine the relationship between muscular fitness indicators in childhood and areal bone mineral density (aBMD) in adulthood and to verify whether the relationship is mediated by performance on muscular fitness indicators in adulthood., Methods: A sample of 138 healthy adults (69 males; 22.3 years) were followed after a previous assessment at the age of 7-10 years. Stature, body mass and muscular fitness indicators (handgrip strength, standing long jump and sit-ups tests) were assessed in childhood and adulthood. Additionally, total body, upper limbs, lower limbs, right femoral neck and lumbar spine aBMD was assessed in adulthood using dual X-ray absorptiometry. Analysis included descriptive statistics; t-test or Mann-Whitney U-test for comparison between males and females, multiple linear regression for the prediction aBMD from muscular fitness indicators in childhood, mediation analysis of the respective muscular fitness indicators in adulthood and the relationship between muscular fitness indicators in childhood and aBMD., Results: Males were stronger compared to females regarding muscular fitness indicators in childhood and adulthood, and presented higher mean values for aBMD in adulthood, except for lumbar spine (p < 0.05). Regression analysis revealed that some muscular fitness indicators in childhood showed significant positive relationship with bone health indicators in adulthood, such as: handgrip strength and total body aBMD (β = 0.005; R
2 = 0.35; p = 0.040) and upper limbs aBMD (β = 0.005; R2 = 0.55; p = 0.019); and sit-ups test was a significant predictors of lumbar spine BMD (β = 0.003; R2 = 0.06; p = 0.039). Mediation analysis pointed out the following: adulthood handgrip strength mediated relationships between childhood handgrip strength and total aBMD (indirect effect (IE) = 0.0025; 95%CI = 0.0005-0.0048), and upper limbs aBMD (IE = 0.0040; 95%CI = 0.0017-0.0069)., Conclusions: Muscular fitness indicators in childhood showed significant relationship with bone health indicators in adulthood and the sit-ups test in childhood had direct effect on lumbar spine aBMD in adulthood. Adulthood handgrip strength mediated the relationship between childhood handgrip strength and total body and upper limb aBMD, pointing out that muscular fitness in childhood may be a aBMD determinant in adulthood, especially when higher muscle fitness performance is maintained in adulthood., (© 2023. The Author(s).)- Published
- 2023
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41. Malathion alters the transcription of target genes of the tumour suppressor tp53 and cancerous processes in Colossoma macropomum: Mechanisms of DNA damage response, oxidative stress and apoptosis.
- Author
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de Souza SS, da Costa JC, da Silva GS, and de Almeida-Val VMF
- Subjects
- Animals, Malathion toxicity, bcl-2-Associated X Protein metabolism, Antioxidants metabolism, Tumor Suppressor Protein p53 genetics, Tumor Suppressor Protein p53 metabolism, Proto-Oncogene Proteins c-bcl-2 genetics, Proto-Oncogene Proteins c-bcl-2 metabolism, Apoptosis, Oxidative Stress, DNA Damage, Neoplasms, Insecticides toxicity
- Abstract
Different classes of pesticides such as fungicides, herbicides, and insecticides, can induce differential expression of genes that are involved in tumorigenesis events in fish, including the expression of tumor suppressor tp53. The degree and duration of the stressful condition is decisive in defining which tp53-dependent pathway will be activated. Herein we evaluate the target genes expression that participates in the regulation pathway of the tumor suppressor tp53 and in the cancerous processes in tambaqui after exposure to malathion. Our hypothesis is that malathion promotes a gene response that is differentially regulated over time, with positive regulation of tp53 target genes related to the apoptotic pathway and a negative regulation of genes that promote antioxidant responses. The fish were exposed to a sublethal concentration of the insecticide for 6 and 48 h. Liver samples were used to analyze the expression of 11 genes using real-time PCR. Overall, the malathion promoted over time increases in tp53 expression and differential expression of tp53 related genes. The exposure resulted in the activation of damage response related genes, caused a positive expression of atm/atr genes. The pro-apoptotic gene bax was up-regulated and the anti-apoptotic bcl2 was down-regulated. Increased expression of mdm2 and sesn1 in the first hours of exposure and no effect on the antioxidant genes sod2 and gpx1 were also observed. We also witnessed an increase in the expression of the hif-1α gene, with no effect on ras proto-oncogene. The extension of this stressful condition accentuated tp53 transcription, and minimized the levels of mdm2, sens1 and bax; however, it down regulated the levels of bcl2 and the bcl2/bax ratio, which indicates the maintenance of the apoptotic response to the detriment of an antioxidant response., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)
- Published
- 2023
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42. Guillain-Barré syndrome associated with COVID-19: A systematic review.
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Pimentel V, Luchsinger VW, Carvalho GL, Alcará AM, Esper NB, Marinowic D, Zanirati G, and da Costa JC
- Abstract
With the outbreak of coronavirus disease 2019 (COVID-19), the whole world was impacted by a pandemic. With the passage of time and knowledge about the dynamics and viral propagation of this disease, the short-, medium- and long-term repercussions are still being discovered. During this period, it has been learned that various manifestations of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can affect the nervous system. In recent months, a variety of studies and case reports have proposed an association between COVID-19 and Guillain-Barré syndrome (GBS). The present work aims to systematically review the publications available to date to verify the relationship between these two pathologies and the characteristics of post-COVID GBS. There were 156 studies included in this work, resulting in a total of 436 patients. The findings show a mean age of the patients of 61,38 years and a male majority. The GBS symptoms began on average 19 days after the onset of COVID-19 infection. Regarding GBS, the main manifestations found included generalized weakness, reflex reduction, facial paresis/paralysis and hypoesthesia. As expected, the most common result in cerebrospinal fluid (CSF) analysis was albuminocytological dissociation. A pattern of blood analysis findings common to all patients was not observed due to non-standardization of case reports. Regarding electrodiagnostic studies, acute inflammatory demyelinating polyneuropathy (AIDP) appeared as the most common subtype of GBS in this study. There have been reports, to a lesser extent, of acute motor axonal neuropathy (AMAN), acute sensorimotor axonal neuropathy (AMSAN), the pharyngeal-cervical-brachial variant (PCB), and Miller-Fisher syndrome (MFS). The GBS treatment used was mainly intravenous immunoglobulin (IVIG) and plasma exchange (PLEX). Therefore, the present study reports a high prevalence of hospitalization and intensive care units ICU admissions, conjecturing a relationship between the development of GBS and the severity of COVID-19. Despite the severity, most patients showed improvement in GBS symptoms after treatment, and their residual symptoms did not include motor involvement. Therefore, the development of GBS seems to be related to COVID-19 infection, as reported by the present systematic review., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2022 Published by Elsevier Inc.)
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- 2023
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43. Body size, maturation and motor performance in young soccer players: relationship of technical actions in small-sided games.
- Author
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da Costa JC, Borges PH, Ramos-Silva LF, Weber VMR, Moreira A, and Vaz Ronque ER
- Abstract
The objective of this study was to investigate the relative contributions of body size, skeletal age, and motor performance variables with technical actions through an ecological model during small-sided soccer games, and the interaction of biological maturation with technical and motor performance in young players. In this cross-sectional study, eighty-two young players (14.4 ± 1.1 years), belonging to state-level soccer teams and divided by category (U-13 and U-15), were included. Players having an injury in the evaluation period were not included in the study. Measurements of body size, skeletal age (SA), motor tests, and technical actions in small-sided games (SSG) were performed (3 × 3 plus goalkeeper) in two periods (halves) of four minutes. Differences between age groups were found for SA (ES = -2.36), chronological age (ES = -3.89), body mass (ES = -2.09), height (ES = -1.90), and fat-free mass (ES = -2.09). Positive associations were found between body size (R = 0.43 to R = 0.48) and manipulation (R = 0.50 to R = 0.52) indicators and numbers of technical actions (CB and SS), except for stature with LB (R = -0.42) in the U-13 age group. In the U-15 category, skeletal age (R = -0.29 to R = -0.30) and body mass (R = -0.28 to R = -0.29) were negatively associated with the number of technical actions (RB, NB, LB, and OB) (P > 0.05) and positively with the balance with LB (R = 0.26). In conclusion, body size, SA, and motor performance influenced technical actions in SSG differentially in each category. U-13 heavier players and those with a better motor performance presented higher involvement due to the higher., Competing Interests: The authors declared no conflict of interests regarding the publication of this manuscript., (Copyright © Biology of Sport 2023.)
- Published
- 2023
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44. Association between cardiorrespiratory fitness and cognitive control: is somatic maturity an important mediator?
- Author
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Weber VMR, da Costa JC, Volpato LA, Romanzini M, Castro-Piñero J, and Ronque ERV
- Subjects
- Humans, Adolescent, Oxygen, Exercise, Cognition, Oxygen Consumption, Cardiorespiratory Fitness
- Abstract
Background: Recently some articles presented information related to the possible effect of maturity over the cognitive control and cardiorespiratory fitness, however little is known about the real effects of maturity in the relation of these variables. In this sense, the purpose of this study was to examine the potential mediating role of somatic maturity on the association between cardiorespiratory fitness (CRF) and cognitive control., Methods: This three-year longitudinal research comprises two data collection groups: a baseline conducted in 2016 with 394 adolescents (aged 11.7 ± 0.6 years) and a follow-up in 2019 with 134 adolescents (aged 14.9 ± 0.7 years). Anthropometry data, 20-m shuttle run test and peak height velocity (PHV) to determine the maximum oxygen uptake (VO
2max ) and somatic maturity, respectively, were collected at both sampling times. In parallel, the Sociodemographic and cognitive control function variables were included in the follow-up to evaluate the inhibitory control (by the Stroop test) and the visuo-spatial working memory (by the Corsi block-tapping test). Associations between CRF and cognitive functions were computed by multiple linear regression, with mediation as a function of PHV., Results: CRF exhibited transversal associations with reaction time in congruent (β = -0.004; p = 0.001) and incongruent (β = -0.005; p = 0.004) stimulus-responses. Meanwhile, the variation in VO2max over the three year-study had a significant impact on the reaction time of congruent (β = -0.006; p = 0.001) and incongruent (β = -0.006; p = 0.012) responses at follow-up. However, PHV did not show a significant association with the cognitive functions, indicating no mediating role., Conclusions: Although the associations between CRF and the cognitive functions exhibited great transversal and longitudinal impacts, somatic maturity did not affect the cognitive control functions, associating exclusively with CRF., (© 2022. The Author(s).)- Published
- 2022
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45. Paecilomyces variotti in deep dental caries.
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Daibert FK, Oliveira MM, Lima-Junior JC, da Costa GL, Alves FR, Gonçalves LS, and Pires FR
- Abstract
Paecilomyces variotti ( P. variotti ) is a fungal species found in soil, wood and some foods, and has been associated with some severe systemic infections. P. variotti has not been previously identified in carious tissue, and the aim of the present study is to report the presence of P. variotti in a deep carious lesion discussing its possible local and systemic associations. A 28 year-old male was submitted to extraction of the upper left second premolar (tooth #25) presenting a deep carious lesion. After extraction the tooth was cleaved in its long axis, and the infected dentinal tissue was curetted and submitted to microbiological analysis using CHROMagar® Candida medium and Malt Extract Agar. Macroscopic and microscopic analysis confirmed the presence of P. variotti in the carious tissue. Post-operatory period was uneventful, healing of the dental socket was complete, and the patient remained well during the follow-up period. P. variotti , a fungus not considered saprophyte in the oral cavity, was encountered in a deep caries lesion, and its potential association with local and systemic infections should be considered. Key words: Paecilomyces variotti, dental caries., Competing Interests: Conflicts of interest The authors declare that they have no conflict of interest., (Copyright: © 2022 Medicina Oral S.L.)
- Published
- 2022
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46. What do we have that is new in antifungal peptides? A patent review.
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Zambrano P, Xavier LC, Santos AM, Rossato L, da Costa JC, Serafini MR, Aragón M, Souto RB, and Alves IA
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- Humans, Peptides pharmacology, Peptides therapeutic use, Antifungal Agents pharmacology, Antifungal Agents therapeutic use, Mycoses drug therapy, Mycoses microbiology
- Abstract
Drugs used to fight fungal infections may cause toxic or adverse drug interactions. For this reason, there is an increase in the development of natural, semisynthetic and synthetic antifungal peptides. This study aimed to perform a patent review to identify the advances in peptides to treat fungal infections. In a preliminary assessment, 597 patents were identified from the database. Then, duplicated patents (62) and those with titles in disagreement with the scope of this review (196) were excluded. Then, six patents were not in English or Spanish. Following the screening, 288 patents were outside the focus of this review, according to their abstract and description. The final selection covered 45 patents.
- Published
- 2022
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47. When to test for COVID-19 using real-time reverse transcriptase polymerase chain reaction: a systematic review.
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Dos Santos PG, Vieira HCVS, Wietholter V, Gallina JP, Andrade TR, Marinowic DR, Zanirati GG, and da Costa JC
- Subjects
- COVID-19 Testing, Clinical Laboratory Techniques methods, Humans, Middle Aged, Prospective Studies, RNA-Directed DNA Polymerase, Real-Time Polymerase Chain Reaction, Retrospective Studies, Reverse Transcriptase Polymerase Chain Reaction, COVID-19 diagnosis
- Abstract
Objectives: The aim of this study was to evaluate the time in days between symptom onset and first positive real-time reverse transcriptase polymerase chain reaction (RT-PCR) result for COVID-19., Methods: This systematic review was conducted in the MEDLINE (PubMed), Embase, and Scopus databases using the following descriptors: "COVID-19", "SARS-CoV-2", "coronavirus", "RT-PCR", "real time PCR", and "diagnosis"., Results: The included studies were conducted in 31 different countries and reported on a total of 6831 patients. The median age of the participants was 49.95 years. The three most common symptoms were fever, cough, and dyspnea, which affected 4012 (58.68%), 3192 (46.69%), and 2009 patients (29.38%), respectively. Among the 90 included studies, 13 were prospective cohorts, 15 were retrospective cohorts, 36 were case reports, 20 were case series, and six were cross-sectional studies. The overall mean time between symptom onset and positive test result was 6.72 days. Fourteen articles were analyzed separately for the temporal profile of RT-PCR test results; the best performance was on days 22-24, when 98% of test results were positive., Conclusion: These findings corroborate the RT-PCR COVID-19 testing practices of some health units. In addition, the most frequently described symptoms of these patients can be considered the initial symptoms of infection and used in decision-making about RT-PCR testing., (Copyright © 2022. Published by Elsevier Ltd.)
- Published
- 2022
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48. Barthel's Index: A Better Predictor for COVID-19 Mortality Than Comorbidities.
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da Costa JC, Manso MC, Gregório S, Leite M, and Pinto JM
- Abstract
Background: The most consistently identified mortality determinants for the new coronavirus 2019 (COVID-19) infection are aging, male sex, cardiovascular/respiratory diseases, and cancer. They were determined from heterogeneous cohorts that included patients with different disease severity and previous conditions. The main goal of this study was to determine if activities of daily living (ADL) dependence measured by Barthel's index could be a predictor for COVID-19 mortality., Methods: A prospective cohort study was performed with a consecutive sample of 340 COVID-19 patients representing patients from all over the northern region of Portugal from October 2020 to March 2021. Mortality risk factors were determined after controlling for demographics, ADL dependence, admission time, comorbidities, clinical manifestations, and delay-time for diagnosis. Central tendency measures were used to analyze continuous variables and absolute numbers (proportions) for categorical variables. For univariable analysis, we used t test, chi-square test, or Fisher exact test as appropriate (α=0.05). Multivariable analysis was performed using logistic regression. IBM SPSS version 27 statistical software was used for data analysis., Results: The cohort included 340 patients (55.3% females) with a mean age of 80.6±11.0 years. The mortality rate was 19.7%. Univariate analysis revealed that aging, ADL dependence, pneumonia, and dementia were associated with mortality and that dyslipidemia and obesity were associated with survival. In multivariable analysis, dyslipidemia (odds ratio [OR], 0.35; 95% confidence interval [CI], 0.17-0.71) was independently associated with survival. Age ≥86 years (pooled OR, 2.239; 95% CI, 1.100-4.559), pneumonia (pooled OR, 3.00; 95% CI, 1.362-6.606), and ADL dependence (pooled OR, 6.296; 95% CI, 1.795-22.088) were significantly related to mortality (receiver operating characteristic area under the curve, 82.1%; p<0.001)., Conclusion: ADL dependence, aging, and pneumonia are three main predictors for COVID-19 mortality in an elderly population.
- Published
- 2022
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49. Combined effect of game position and body size on network-based centrality measures performed by young soccer players in small-sided games.
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Borges PH, da Costa JC, Ramos-Silva LF, Praça GM, and Ronque ERV
- Abstract
This study verified the effects of body size and game position on interactions performed by young soccer players in small-sided games (SSG). The sample consisted of 81 Brazilian soccer players (14.4 ± 1.1 years of age). Height, body mass, and trunk-cephalic height were measured. SSG was applied in the GK + 3v3 + GK format, and Social Network Analyses were carried out through filming the games to obtain the following prominence indicators: degree centrality, closeness centrality, degree prestige, and proximity prestige, in addition to network intensity and number of goals scored. Factorial ANCOVA (bone age as covariate) was used to test the effects of game position, body size, and respective interaction on centrality measurements ( p < 0.05). Similarity between game positions in body size indicators ( p > 0.05) was observed. The game position affected degree centrality ( p = 0.01, η
2 = 0.16), closeness centrality ( p = 0.01, η2 = 0.11), and network intensity ( p = 0.02, η2 = 0.09), in which midfielders presented the highest network prominence values when compared to defenders and forwards. In conclusion, midfielders are players with high interaction patterns in the main offensive plays, which behavior is independent of body size., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be considered as a potential conflict of interest., (Copyright © 2022 Borges, Costa, Ramos-Silva, Praça and Ronque.)- Published
- 2022
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50. Clozapine induces astrocyte-dependent FDG-PET hypometabolism.
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Rocha A, Bellaver B, Souza DG, Schu G, Fontana IC, Venturin GT, Greggio S, Fontella FU, Schiavenin ML, Machado LS, Miron D, da Costa JC, Rosa-Neto P, Souza DO, Pellerin L, and Zimmer ER
- Subjects
- Animals, Fluorodeoxyglucose F18 metabolism, Glucose metabolism, Glutamic Acid metabolism, Glutamic Acid pharmacology, Humans, Male, Positron-Emission Tomography, Rats, Rats, Wistar, Astrocytes, Clozapine metabolism, Clozapine pharmacology
- Abstract
Purpose: Advances in functional imaging allowed us to visualize brain glucose metabolism in vivo and non-invasively with [
18 F]fluoro-2-deoxyglucose (FDG) positron emission tomography (PET) imaging. In the past decades, FDG-PET has been instrumental in the understanding of brain function in health and disease. The source of the FDG-PET signal has been attributed to neuronal uptake, with hypometabolism being considered as a direct index of neuronal dysfunction or death. However, other brain cells are also metabolically active, including astrocytes. Based on the astrocyte-neuron lactate shuttle hypothesis, the activation of the glutamate transporter 1 (GLT-1) acts as a trigger for glucose uptake by astrocytes. With this in mind, we investigated glucose utilization changes after pharmacologically downregulating GLT-1 with clozapine (CLO), an anti-psychotic drug., Methods: Adult male Wistar rats (control, n = 14; CLO, n = 12) received CLO (25/35 mg kg-1 ) for 6 weeks. CLO effects were evaluated in vivo with FDG-PET and cortical tissue was used to evaluate glutamate uptake and GLT-1 and GLAST levels. CLO treatment effects were also assessed in cortical astrocyte cultures (glucose and glutamate uptake, GLT-1 and GLAST levels) and in cortical neuronal cultures (glucose uptake)., Results: CLO markedly reduced in vivo brain glucose metabolism in several brain areas, especially in the cortex. Ex vivo analyses demonstrated decreased cortical glutamate transport along with GLT-1 mRNA and protein downregulation. In astrocyte cultures, CLO decreased GLT-1 density as well as glutamate and glucose uptake. By contrast, in cortical neuronal cultures, CLO did not affect glucose uptake., Conclusion: This work provides in vivo demonstration that GLT-1 downregulation induces astrocyte-dependent cortical FDG-PET hypometabolism-mimicking the hypometabolic signature seen in people developing dementia-and adds further evidence that astrocytes are key contributors of the FDG-PET signal., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)- Published
- 2022
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