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Simple Summary: Canine high-grade glioma (HGG) is a deadly disease in dogs, with limited treatment options. Involving the host immune system in the fight against HGG through immunotherapy has improved outcomes in many dogs, with a notable exception in the French bulldog breed. We hypothesized that French bulldog HGG responds differently to immunotherapy than other breeds, and that this response difference is attributable to differences in transcriptomic landscapes in the breed compared to other breeds. We performed bulk RNA sequencing (RNASeq) on tumors pre-treatment and post-treatment and examined the differentially expressed genes (DEGs) between the two timepoints in both French bulldogs and in our comparison group of boxers and Boston terriers. We then analyzed the differences in DEGs between French bulldogs and boxers and Boston terriers, and performed gene set enrichment analysis (GSEA) to categorize the observed changes by gene function. We confirmed that when compared to boxers and Boston terriers, the transcriptomes of French bulldog tumors change in significantly different ways following immunotherapy treatment, with many DEGs mapping to tumor-promoting pathways and immune pathways. This study highlights important breed-associated differences in HGG transcriptomes, which may guide patient-centered immunotherapy treatment to improve patient outcomes. Canine high-grade glioma (HGG) is among the deadliest and most treatment-resistant forms of canine cancer. Successful, widespread treatment is challenged by heterogeneity in tumor cells and the tumor microenvironment and tumor evolution following treatment. Immunotherapy is theoretically a strong novel therapy, since HGG-generated immunosuppression is a substantial malignancy mechanism. Immunotherapy has improved survival times overall, but has been associated with extremely poor outcomes in French bulldogs. Given this breed-specific observation, we hypothesized that within the French bulldog breed, there are key transcriptomic differences when compared to other breeds, and that their tumors change differently in response to immunotherapy. Using bulk RNA sequencing, French bulldog tumors were confirmed to differ substantially from boxer and Boston terrier tumors, with only 15.9% overlap in significant differentially expressed genes (DEGs). In upregulated DEGs, the magnitude of changes in expression post-treatment compared to pre-treatment was markedly greater in French bulldogs. Gene set enrichment analysis confirmed that following treatment, French bulldog tumors showed enrichment of key immune-associated pathways previously correlated with poor prognosis. Overall, this study confirmed that French bulldog HGG transcriptomes differ from boxer and Boston terrier transcriptomes, further refining description of the canine glioma transcriptome and providing important information to guide novel therapy development, both for specific dog breeds and for possible correlative variants of human glioblastoma. [ABSTRACT FROM AUTHOR]

Simple Summary: This study explored how well French Bulldogs understand nonverbal communication, specifically whether having a close bond with their owner helps them interpret pointing gestures. Researchers worked with 26 French Bulldogs and their owners, testing whether the dogs could follow their owner's pointing to find a treat hidden in a bowl. To measure the bond between each dog and their owner, they used a questionnaire and checked the dogs' salivary oxytocin levels, a hormone linked to positive emotions. The results showed that factors like the dog's age, gender, neuter status, and training experience affected how well they performed. Dogs with stronger bonds with their owners were generally better at understanding the pointing gestures, although oxytocin levels didn't directly influence their choices. The authors suggest that future research should include more dog breeds to better understand how the owner–dog relationship influences social skills over time. Our understanding of social cognition in brachycephalic dog breeds is limited. This study focused specifically on French Bulldogs and hypothesized that a closer relationship between dog and owner would improve the dogs' understanding of nonverbal cues, particularly pointing gestures. To investigate this, we tested twenty-six dogs and their owners in a two-way object choice test in which the familiar person pointed to the bowl. As understanding of pointing gestures is influenced by human–animal interaction, we used the Monash Dog Owner Relationship Scale (MDORS) to assess various aspects of the dog–owner relationship, including dog–owner interaction, emotional closeness, and negative aspects of dog ownership and measured salivary oxytocin levels. This study examined the dogs' correct choices and their straight approach patterns to a reward bowl. Several factors influenced the two measures of social cognition, such as the age and sex of the dog, dog–owner interaction, emotional closeness, and the choice time (time it took the dog to reach the reward bowl), but also that neutered dogs and those with no training history showed better cognitive performance. We found a very high number of correct choices, which could be attributed to the owners' high scores on the dog–owner interaction scale. Oxytocin showed no direct effect on these measures. To increase the generalizability of the results, we suggest future studies focus on more than one breed to capture the temporal development of the owner–dog relationship and social cognitive abilities. [ABSTRACT FROM AUTHOR]

Simple Summary: Taurine is essential for the health of small animals, particularly dogs, as a deficiency can lead to problems like impaired foetal development, retinal degeneration, and cardiomyopathy in certain breeds. This study presents a new validated liquid chromatography–tandem mass spectrometry (LC-MS/MS) method for fast and accurate taurine measurement in canine whole blood and plasma, with limits of 80 nmol/mL and 8 nmol/mL, respectively. The method allowed us to establish reliable reference intervals for healthy dogs and offered a practical and robust tool for routine veterinary use for monitoring taurine concentrations effectively. Several studies have highlighted the essential role of taurine in maintaining the health of small animals, particularly dogs. Taurine deficiency has been linked to various health issues, especially in certain dog breeds. Therefore, accurately assessing taurine levels in canine blood is crucial for diagnosing and monitoring these conditions. In this study, we present the development of a novel liquid chromatography–tandem mass spectrometry (LC-MS/MS) method for rapidly quantifying taurine concentrations in dog whole blood and plasma. The method was validated according to current guidelines, showing excellent accuracy, precision, and sensitivity across a wide concentration range. Specifically, the limit of quantification was set at 80 nmol/mL for whole blood and 8 nmol/mL for plasma, ensuring the method's reliability for both matrices. The application of this validated technique to blood samples of healthy dogs allowed for the establishment of reference intervals for taurine concentrations (148 to 495 nmol/mL for whole blood; 42 to 183 nmol/mL for plasma). Due to its robustness and simplicity, this method represents a valuable tool, supporting its routine use in health assessments and enabling more effective monitoring of taurine status in dogs. [ABSTRACT FROM AUTHOR]

Background/Objectives: The domestication of the grey wolf (Canis lupus) and subsequent creation of modern dog breeds have significantly shaped the genetic landscape of domestic canines. This study investigates the genomic effects of hybridization and breeding management practices in two hybrid wolfdog breeds: the Czechoslovakian Wolfdog (CSW) and the Saarloos Wolfdog (SAW). Methods: We analyzed the genomes of 46 CSWs and 20 SAWs, comparing them to 12 German Shepherds (GSHs) and 20 wolves (WLFs), which served as their ancestral populations approximately 70–90 years ago. Results: Our findings highlight that hybridization can increase genetic variability and mitigate the effects of inbreeding, as evidenced by the observed heterozygosity levels in both wolfdog breeds. However, the SAW genome revealed a higher coefficient of inbreeding and longer runs of homozygosity compared to the CSW, reflecting significant inbreeding during its development. Discriminant Analysis of Principal Components and fixation index analyses demonstrate that the CSW exhibits closer genetic proximity to the GSH than the SAW, likely due to differences in the numbers of GSHs used during their creation. Maximum likelihood clustering further confirmed clear genetic differentiation between these hybrid breeds and their respective ancestors, while shared ancestral polymorphism was detectable in all populations. Conclusions: These results highlight the role of controlled hybridization with captive-bred wolves and peculiar breeding strategies in shaping the genetic structure of wolfdog breeds. To ensure the long-term genetic health of these breeds, it is recommended to promote adequate and sustainable breeding practices to maintain genetic diversity, minimize inbreeding, and incorporate the careful selection of unrelated individuals from diverse lineages, while avoiding additional, uncontrolled crossings with wild wolves. [ABSTRACT FROM AUTHOR]

Introduction: Congenital vertebral malformations are common developmental abnormalities in screw-tailed brachycephalic dog breeds. Subsequent vertebral instability and/or vertebral canal stenosis caused by these malformations can lead to spinal cord compression manifesting in pain, paraparesis, ataxia and/or paralysis. Various methods for spinal stabilization are in common use. However, these are without significant risk due to narrow margins of surgical error and variable vertebral anatomy. We evaluate a novel method for spinal stabilization where a custom 3D-printed plate is created and surgically fitted to the patient's spine using custom 3D-printed drill guides. Objective: To describe the surgical technique and short-term outcomes in patients treated with custom 3D-printed plates and drill guides. Method: A retrospective analysis of 11 dogs from two referral hospitals which underwent this procedure was undertaken. Post-operative CT scans were assessed for spinal canal screw perforation using the modified Zdichavsky classification. Pre-operative and post-operative neurological status were assessed using the Modified Frankel Scale and the surgical technique including post-operative imaging and recovery findings were described. Results: Optimal screw placement (grade I) was achieved in 63% of placed screws across the eleven dogs. Partial penetration of the medial wall (grade IIa) was observed in 3% of screws and partial penetration of the lateral wall (grade IIIa) was observed in 29% of screws. Full penetration of the lateral pedicle wall (grade IIIb) was observed in 5% of screws and no screws fully penetrated the medial vertebral wall (grade IIb). Discussion: We demonstrated that custom 3D-printed drill guides and titanium plates can provide a safe peri-operative alternative for surgical spinal stabilization of dogs with vertebral column instability due to congenital vertebral malformations. Further research is needed to describe long-term outcomes of this surgical technique on patient health. [ABSTRACT FROM AUTHOR]

Fibrolipoma is defined as a typical lipoma transected by variable amounts of paucicellular and collagenous fibrous components. Oral and lingual fibrolipomas are well-recognized histological entities in human medicine that are slightly more prevalent in females, occur most commonly after the fourth decade, and arise from the buccal mucosa. The documentation of this neoplasm in the oral cavity is lacking in veterinary medicine. Through a multi-institutional retrospective compilation of cases submitted to diagnostic pathology services, here we describe the clinical and pathologic features of oral fibrolipomas in dogs. A total of 112 cases of oral fibrolipomas in dogs were retrieved. The mean age was 10.1 years (range 2–16 years, ±2.63 years standard deviation), with an average tumor size of 1.7 cm (range 0.2–8 cm, ±1.1 cm standard deviation). The most common location was the tongue (57.1%, 64/112), followed by the buccal mucosa (15.2%, 16/112), sublingual area (8.0%, 9/112), gingiva and lip (4.5%, 5/112 each), and palate (1 case). The anatomical location of oral fibrolipomas only differed significantly among the dog breeds (P <.001) but not among sex, age, anamnesis, or reason for submission. The tumor was most commonly reported in males (69.7%, 78/112), and in 62.5% (70/112) of the cases, the tumor was an incidental finding. Fibrolipoma should be considered a differential diagnosis when considering benign lingual and other oral soft tissue masses in dogs. [ABSTRACT FROM AUTHOR]

Brachycephalic obstructive airway syndrome (BOAS) is well documented in the three most popular brachycephalic dog breeds of the UK and several other countries: French Bulldogs, Pugs and Bulldogs. More extreme conformation has been found to be associated with increased risk of BOAS and other brachycephalic disease in these breeds, such as ocular, neurological, and dental disease. Less is known about how BOAS and other brachycephalic conformation-related disease affects other breeds such as the Boston Terrier. In this study, one-hundred and seven Boston Terriers were prospectively recruited from the UK dog population and underwent clinical assessment, respiratory function grading and conformational measurements. Whole-body barometric plethysmography was used in a smaller cohort of dogs to compare the quantitative differences in respiratory parameters between both affected and unaffected Boston Terriers, and control mesocephalic dogs. When compared to an equivalent study population of French Bulldogs and Bulldogs, it was found that Boston Terriers have a significantly higher proportion of BOAS Grade 0 dogs at 37.5% compared to 10% and 15.2% respectively (p<0.01). Within the breed, more extreme brachycephalic conformation was found to be associated with an increased risk of BOAS: specifically, nostril stenosis, facial foreshortening, abnormal scleral show, and higher neck to chest girth ratio. However, there is considerable overlap between measurements of affected and unaffected dogs in these variables. Therefore, the use of respiratory function grading is likely to be more advantageous for owners, breeders, and veterinary surgeons in accurately selecting unaffected dogs. [ABSTRACT FROM AUTHOR]

There is limited knowledge about the size of the UK dog population. This makes it difficult to reliably monitor population dynamics and management. A repeatable method of measuring the UK dog population, including owned and unowned dogs i.e., those housed long term by animal welfare charities, would help inform interventions to reduce risks to dog welfare such as poor breeding practices and the illegal import of young or pregnant dogs. However, estimating the total dog population in the UK is not straightforward. Although several estimates of dog numbers have been previously suggested, differing methodologies and limited public access to data sources makes comparison and replication difficult. In this study, we compiled a comprehensive dataset of UK pet dogs in 2019, from multiple sources, including a breed registry, veterinary corporations, pet insurance companies, animal welfare charities and an academic institution. Using a hierarchical Bayesian N-mixture model, we estimate 12.64 million (mean, 95% CI 8.54–15.16 million; median, 13.03 million, 95% CI 8.51–15.24 million) owned and unowned pet dogs within the UK in 2019. Estimates were modelled at the postcode area scale, allowing for aggregation to region or country level, as well as providing associated metrics of uncertainty. Furthermore, we provide spatial demographic estimates, regarding age, breed, cephalic index, and body size. Establishing a population baseline offers significant analytical benefits to welfare, veterinary, epidemiological, and business stakeholders alike: as it provides the spatial data required to underpin robust canine welfare strategies and campaigns. We plan to repeat the described process, in order to provide ongoing estimates: allowing for the interrogation of changes to the UK pet dog population over space and time. [ABSTRACT FROM AUTHOR]

Simple Summary: Within the elderly dog population, not all dogs of the same breed at the same age have the same vulnerability to the future development of adverse health outcomes. Understanding this biological heterogeneity is one key to advancing the health of geriatric pets. The quantitation of an individual's health deficits using a clinical tool called frailty index (FI) has been applied to aging human populations. Two previous studies have reported FI in dogs; neither focused on geriatric dogs. Our aim was to capitalize on The Exceptional Aging in Rottweilers Study (EARS), the first study of pet dogs living in North America that have reached extreme longevity, by developing an FI in geriatric dogs and then testing this tool as a predictor of mortality. We constructed a clinical frailty index, EARS-FI, using information attained through telephone interviews with owners of 93 dogs. Using EARS-FI, we showed these dogs have a broad range of FI values, an increase in frailty with increasing age, and an upper FI limit. Notably, we provide the first demonstration of increased mortality risk associated with increasing frailty in pet dogs with extreme longevity. The work has important implications for both pet owners and One Health investigators. EARS-FI should provide a useful tool for further in-depth analyses of linkages between health deficit accumulation and adverse clinical outcomes. This research benefits geriatric pets by contributing to a better understanding of frailty in aging dogs and sets the stage for identifying factors that undermine optimal trajectories of lifetime physical health. Frailty refers to a state of increased vulnerability to mortality and other adverse outcomes as a consequence of age-related decline in physiologic reserve and function. Comparative biomedical scientists are relied upon to innovate approaches to enhance understanding of the similarities and differences between humans and other animal species that can impact healthy aging. The research aim of this study was to develop a clinical frailty index (FI) in the Exceptional Aging in Rottweilers Study (EARS) and test its ability to predict all-cause mortality in elderly dogs. EARS is an ongoing lifetime cohort study of pet dogs with extreme longevity living in North America. Living 30% longer than the breed average, these dogs represent the canine counterpart to human centenarians. A 34-item FI (EARS-FI) was constructed to assess deficit accumulation using clinical data collected by telephone interviews with owners of 93 dogs with extreme longevity. Health deficits across multiple domains, including cognitive and sensory, cardiovascular and endocrine, and mobility, were included. The association between EARS-FI and subsequent mortality was tested in Kaplan-Meier survival analysis and in age-adjusted Cox proportional hazard models. Median (interquartile range) EARS-FI was 0.43 (0.38–0.50), and the estimated frailty limit was 0.68, consistent with data reported in humans with extreme longevity. Frailty index increased with increasing chronological age (p < 0.001). Deficit accumulation was significantly associated with increased mortality risk. Age-adjusted hazard ratio for mortality per 0.01 unit increase in FI was 1.05 (95%CI, 1.02–1.08; p = 0.001). This work provides the first demonstration of a strong association between frailty and mortality risk in pet dogs with extreme longevity. Notably, EARS-FI showed key features observed in the evaluation of frailty in aging human populations: heterogeneity, increase with chronological age, and estimated limit of <0.7. Validated here as a predictor of mortality in aged pet dogs, EARS-FI offers a useful tool for further comparative analyses of the linkages between deficit accumulation, mortality, and other adverse health outcomes. [ABSTRACT FROM AUTHOR]

Simple Summary: This study compared the muscle fiber composition of working and companion dog breeds to understand how their muscles adapt to breed-specific tasks. By analyzing the triceps brachii and biceps femoris muscles of 12 dogs, we found that working dogs had larger muscle fibers, a higher proportion of endurance-related fibers (types IIa and I), and more nuclei per fiber, which support strength and endurance. Companion dogs, on the other hand, had more fibers suited for short bursts of activity (type IIb) and fewer nuclei per fiber, suggesting slower muscle regeneration. These results highlight the role of selective breeding in shaping muscle structure and function in dogs. The structural and functional characteristics of skeletal muscle fibers play a crucial role in understanding the physical capabilities of dogs, particularly in relation to their breed-specific roles. This study aimed to compare the muscle fiber composition of working and companion dog breeds by analyzing the triceps brachii and biceps femoris muscles, focusing on fiber morphology, myosin heavy chain (MYH) isoform distribution, and nuclei per fiber. A total of 12 dogs, divided equally into working and companion breed groups, were used in this study. Muscle samples were collected post-mortem and prepared for histological analysis using cryosectioning. Immunohistochemical staining was employed to identify the expression of MYH isoforms, including MYH2, MYH4, and MYH7, which correspond to type IIa, IIb, and type I fibers, respectively. The results demonstrated significant differences between the two breed groups. Working dogs exhibited larger muscle fibers, a higher proportion of type IIa (MYH2) and type I (MYH7) fibers, and a greater number of nuclei per fiber, suggesting adaptations for endurance and strength. In contrast, companion dogs showed a higher proportion of type IIb (MYH4) fibers, indicative of their capacity for short bursts of activity rather than sustained exertion. Companion breeds also displayed a higher fiber density but fewer nuclei per fiber, which may contribute to slower muscle regeneration. These findings may provide insights into the muscle adaptations of dogs based on their breed-specific functional demands and highlight the importance of considering these differences in veterinary care and rehabilitation. The study underscores the influence of selective breeding on muscle structure and function in dogs and suggests further research into breed-specific muscle recovery mechanisms. [ABSTRACT FROM AUTHOR]

Canine Hip Dysplasia (CHD) is a congenital disease with a polygenic hereditary component, characterised by abnormal development of the coxo-femoral joint which results in poor coaptation of the femoral head in the acetabulum; the disease rapidly progresses to osteoarthritis of the hip. While dysplasia has been recognised in practically all canine breeds, it is much more common and of concern in medium and large dog breeds with rapid development. Dysplasia in predisposed breeds, particularly the German Shepherd, is the object of screening based on systematic radiological control in some countries. Our collected dataset comprises 507 X-ray images of dogs affected by hip dysplasia (HD). These images were meticulously evaluated using six Deep Convolutional Neural Network (CNN) models. Following an extensive analysis of the top-performing models, VGG16 emerged as the leader, achieving remarkable accuracy, recall, and precision scores of 98.32%, 98.35%, and 98.44%, respectively. Leveraging deep learning (DL) techniques, this approach excels in diagnosing CHD from hip X-rays with a high degree of accuracy. [ABSTRACT FROM AUTHOR]

The "VCS 2024 Abstracts" from the Veterinary & Comparative Oncology journal features research on various topics in veterinary oncology, including the diagnosis of lipomas in dogs, genetic alterations in canine osteosarcoma, and tolerability evaluation of combination doxorubicin and verdinexor in tumor-bearing dogs. Studies also explore PARP inhibition in feline oral squamous cell carcinoma treatment, tumor-infiltrating lymphocyte density in canine adenocarcinoma, and outcomes following radiation therapy for dogs with cardiac hemangiosarcoma. The research provides valuable insights into cancer treatment and prognosis in veterinary medicine, with promising results in EGFR/HER2 immunotherapy for canine cancers. Additionally, a study on genetic dependencies in feline oral squamous cell carcinoma using CRISPR-based screening aims to identify new therapeutic targets for this type of cancer. [Extracted from the article]

Domestication process effects are manifold, affecting genotype and phenotype, and assumed to be universal in animals by part of the scientific community. While mammals and birds have been thoroughly investigated, from taming to intensive selective breeding, fish domestication remains comparatively unstudied. The most widely bred and traded ornamental fish species worldwide, the goldfish, underwent the effect of long-term artificial selection on differing skeletal and soft tissue modules through ornamental domestication. Here, we provide a global morphological analysis in this emblematic ornamental domesticated fish. We demonstrate that goldfish exhibit unique morphological innovations in whole-body, cranial, and sensory (Weberian ossicles and brain) anatomy compared to their evolutionary clade, highlighting a remarkable morphological disparity within a single species comparable to that of a macroevolutionary radiation. In goldfish, as in the case of dogs and pigeons in their respective evolutionary contexts, the most ornamented varieties are extremes in the occupied morphological space, emphasizing the power of artificial selection for nonadaptive traits. Using 21st century tools on a dataset comprising the 16 main goldfish breeds, 23 wild close relatives, and 39 cypriniform species, we show that Charles Darwin's expressed wonder at the goldfish is justified. There is a commonality of overall pattern in the morphological differentiation of domesticated forms selected for ornamental purposes, but the singularity of goldfish occupation and extension within (phylo)morphospaces, speaks against a universality in the domestication process. [ABSTRACT FROM AUTHOR]

The enactment of the Dangerous Dogs Act 1991 is an example of a reactive introduction of legislation in response to a series of dog attacks that year. In September 2023, the Prime Minister, Rishi Sunak, announced that the Government would again be taking action to ban an additional dog breed type by the end of 2023 in another reactive action. The proposal to add the American Bully XL breed type to the list of banned breeds detailed in S.1 Dangerous Dogs Act 1991 came into force on 31 December 2023. No other proposals for reform of the Act have been suggested to date. This article will explore the legislative amendments and consider whether the Government's recent reform is sufficient to adequately protect the public from dangerous dogs and dog attacks. [ABSTRACT FROM AUTHOR]

The exam for the General Part of the German Civil Code (BGB AT) at the Ruprecht-Karls-University Heidelberg in the summer semester of 2023 dealt with issues of representation and association law, in particular the founding of a dog sports club and the acquisition of a property. Questions regarding claims of E against V, T against V, and F against V were discussed, including possible claims for damages by T against V due to lack of information about the representative authority. The applicability of § 31 BGB, the liability of the association for actions of its representatives, and the approval of contracts were also discussed, as well as claims of F against V due to withdrawn membership fees and tortious claims for damages. [Extracted from the article]

Background: Canine behavior plays an important role in the success of the human–dog relationship and the dog's overall welfare, making selection for behavior a vital part of any breeding program. While behaviors are complex traits determined by gene × environment interactions, genetic selection for desirable behavioral phenotypes remains possible. Methods: No genomic association studies of dog behavior to date have been reported on a commercial breeding (CB) cohort; therefore, we utilized dogs from these facilities (n = 615 dogs). Behavioral testing followed previously validated protocols, resulting in three phenotypes/variables [social fear (SF), non-social fear (NSF), and startle response (SR)]. Dogs were genotyped on the 710 K Affymetrix Axiom CanineHD SNP array. Results: Inbreeding coefficients indicated that dogs from CB facilities are statistically less inbred than dogs originating from other breeding sources. Heritability estimates for behavioral phenotypes ranged from 0.042 ± 0.045 to 0.354 ± 0.111. A genome-wide association analysis identified genetic loci associated with SF, NSF, and SR; genes near many of these loci have been previously associated with behavioral phenotypes in other populations of dogs. Finally, genetic risk scores demonstrated differences between dogs that were more or less fearful in response to test stimuli, suggesting that these behaviors could be subjected to genetic improvement. Conclusions: This study confirms several canine genetic behavioral loci identified in previous studies. It also demonstrates that inbreeding coefficients of dogs in CB facilities are typically lower than those in dogs originating from other breeding sources. SF and NSF were more heritable than SR. Risk allele and weighted risk scores suggest that fearful behaviors could be subjected to genetic improvement. [ABSTRACT FROM AUTHOR]

To investigate the effect of individuals' personality on their dog breed choices, an online questionnaire was conducted with 581 dog keepers. Breeds were classified according to body size, functions, temperament, and ban status. Personality classifications of keepers were achieved through the Eysenck Personality Questionnaire in the Turkish language. To determine effects of individuals' personality on breed choices, the General Linear Model ANOVA and Kruskal Wallis analysis were applied. The result revealed no personality differences between keepers when dogs were grouped by body size, temperament, or function. In terms of the "lie" subscale, there was a difference between keepers who chose to have a banned dog breed versus those who did not. Results showed that keepers who scored lower on the "lie" subscale were found to prefer banned dog breeds. The outcomes of this study will help to better understand personality differences among dog keepers and their choices of dog breeds. [ABSTRACT FROM AUTHOR]

Background and Aim: Canine degenerative myelopathy (DM) is an autosomal recessive inherited disease that affects different dog breeds. It has an invariably fatal outcome once the clinical symptoms begin. This study aimed to investigate the population behavior of the mutation superoxide dismutase 1 (SOD1) c.118: G>A responsible for the high risk of developing DM in two populations of German Shepherd dogs from Uruguay and Paraguay. Materials and Methods: A total of 158 German Shepherd dogs from Uruguay (n = 114) and Paraguay (n = 44) were analyzed. Genomic DNA was extracted from peripheral whole blood. The SOD1 c.118: G>A mutation was identified by polymerase chain reaction-restriction fragment length polymorphism and subsequently validated using sequencing. Allelic and genotypic frequencies and Hardy-Weinberg equilibrium were calculated for both populations. The rate of clinical progression was evaluated in animals homozygous for the mutation. Results: The frequencies of allele A associated with a higher risk of DM, were 0.15 and 0.23 in Paraguay and Uruguay, respectively. Paraguay's population was found to be in Hardy-Weinberg equilibrium (p = 1.00), whereas the population of dogs from Uruguay deviated from equilibrium (p = 0.008). When comparing the populations, no significant difference was observed in the distribution of genotypes (p = 0.26). When evaluating the clinical progression rate, all animals aged >10 years showed clinical symptoms compatible with DM. Conclusion: This study demonstrated for the first time the presence of the SOD1:c118 G>A mutation in German Shepherd dogs from Uruguay and Paraguay. The frequency detected in Uruguay was significant. Although the frequency was lower in Paraguay, the allele was present. This demonstrates the need to implement genotyping tests as part of a possible DM control program in both countries studied. [ABSTRACT FROM AUTHOR]

Simple Summary: The disease investigation of companion animals is important for the clinical management of disease as well as the control of disease risk factors. The Bullmastiff is a giant brachycephalic dog breed which has been reported to have an increased risk of lymphoma, musculoskeletal and ocular disorders, as well as a shortened lifespan, although an in-depth disease investigation of the Bullmastiff has not been conducted. This study aimed to provide a comprehensive retrospective study of the breed, investigating the demography, longevity and mortality of Bullmastiffs attending veterinary practices in Australia over a ten-year period from 2008 to 2017. This was achieved through the systematic review of patient records from veterinary practices participating in the VetCompass research collaboration. This study described the demography, body weight, longevity and mortality of the Bullmastiff, revealing the study population to have a median age of 2.8 years and a mean body weight of 46.6 kg for males and 40.5 kg for females. The dogs had a median longevity of 8.5 years, and the major causes of mortality were mass lesions, old age and musculoskeletal, neurological and behavioural disorders. Desexing was found to significantly reduce the risk of mortality due to urogenital causes. The results of this study may assist in the veterinary care, health management, breeding guidelines and welfare of Bullmastiffs. The Bullmastiff is a giant breed of dog, and there is evidence the breed is predisposed to cancer and musculoskeletal disorders, though the disease investigation of the breed is limited. This study reports on the demography, longevity and mortality of Bullmastiffs attending veterinary practices in Australia over a ten-year period. VetCompass Australia collects patient data from veterinary practices across Australia for epidemiological analysis. All patient records of Bullmastiffs available in the VetCompass Australia database during this decade period were reviewed, with demographic information on the breed inclusive of coat colour, sex, neuter status, weight and location collated. Standardised veterinary diagnostic (VeNom) codes for the most appropriate cause of death were assigned to deceased dogs. The population comprised 2771 Bullmastiffs with an overall median age of 2.8 years. Within the group, 1259 were female (45.4%), 1491 were male (53.8%), and 21 dogs (0.8%) had no recorded sex or neuter status. Dogs grew rapidly in their first year, with an average gain of approximately 1 kg every 10 days. A slower growth rate continued in their second year, and growth plateaued as adulthood was reached, with the mean body weight of adult male dogs (46.6 kg) being heavier than that of females (40.5 kg). The age at death for the group was 8.5 years. The most common causes of death in the breed were mass lesions (28.2%), old age (9.9%), musculoskeletal-related disease (9.9%) and neurological (5.3%) and behavioural disorders (4.8%). Neutering was protective against mortality from urogenital causes (OR: 0.14; CI: 0.02–0.52; p = 0.003) and had a positive effect on longevity. This study provides demographic and health information on a population of Bullmastiffs attending veterinary practices in Australia, which will benefit evidence-based veterinary decisions for this breed. Additionally, the results may assist owners and breeders in making informed decisions on health risks and breeding programmes in the population. [ABSTRACT FROM AUTHOR]

Hereditary sensory and autonomic neuropathies (HSAN) represent a group of genetic diseases affecting the peripheral nervous system. In humans, at least 16 loci have been associated with the disorder but do not explain the disease origin of all patients. In dogs, similar conditions have been documented for decades in various breeds with a severe impact on life quality and are often referred to as acral mutilation syndrome (AMS). Causal variants in three genes have been identified to date, suggesting larger genetic heterogeneity in the dog population. Our aim was to explain the genetic etiology of an early‐onset HSAN/AMS in a purebred German Spitz. The affected dog showed progressive loss of pain sensation in the distal extremities, which led to intense licking, biting, and self‐mutilation of digits and paw pads. Whole‐genome sequencing identified a single candidate causal variant on chromosome 4 in the RETREG1 gene (c.656C>T, p.Pro219Leu). This missense variant was previously recognized as deleterious in a mixed breed dog family with similar clinical signs. Haplotype analyses and targeted genotyping revealed a likely German Spitz ancestry of these mixed breed dogs. Further screening of an extensive cohort of ~900 000 dogs of various breeds hinted at the variant allele origin in the German Spitz breed. Disruption of RETREG1 inhibits endoplasmic reticulum turnover and leads to neuron degeneration. Our findings provide evidence that this variant underlies the recessive form of HSAN/AMS in the German Spitz and support the use of whole‐genome sequencing‐based veterinary precision medicine for early diagnosis and prevention via a genetic test. [ABSTRACT FROM AUTHOR]

Assessment of individual behavioral characteristics (IBC) of domestic dogs is of both fundamental and practical interest to researchers. We described in this work the development and assessment of the reliability of a methodology for assessing IBC based on an online survey of owners who did not have special training. Four thousand five hundred thirty respondents took part in the survey. The questionnaire included 20 questions, which were rated on a Likert scale. Using the factor analysis we identified 6 factors, by which the IBC of different breed groups were compared according to the FCI classification, and the prognostic role of breed in determining differences in the IBC of dogs was assessed. A comparison of the data from our questionnaire with similar ones obtained in the United States and Japan (Q-BARK) showed that the identified factors generally coincided (Cronbach's Alpha was 0.73, which indicated sufficient reliability of the methodology), although the questions for the Russian audience were formulated differently. Thus, this questionnaire can be used to assess the IBC, the formation of a modern behavioral portrait of a particular breed, its compliance with existing standards, as well as changes that occur as a result of the lack of selection, for example, for working qualities. [ABSTRACT FROM AUTHOR]

Simple Summary: Canine hip dysplasia is a painful condition common in large dog breeds, leading to joint instability and osteoarthritis. Accurate diagnosis is crucial for guiding breeding decisions to help reduce its prevalence. However, evaluating hip health status can be challenging, as minor positioning changes during X-rays can distort images and hinder proper hip joint assessment. In this study, we developed an artificial intelligence tool for the automatic evaluation of pelvic alignment in X-rays. By detecting subtle asymmetries in bone structure, the tool determines whether a dog's hips are properly aligned. Our findings showed that the artificial intelligence tool performed as accurately as an expert human examiner in detecting misalignment. This automated approach could improve the reliability of canine hip dysplasia screenings, saving veterinarians time and reducing misdiagnosis risks due to human error. Ultimately, this technology has the potential to enhance medical care for dogs and support breeders in making more informed choices, contributing to canine health improvements and reducing canine hip dysplasia incidence in future generations. Canine hip dysplasia (CHD) screening relies on accurate positioning in the ventrodorsal hip extended (VDHE) view, as even mild pelvic rotation can affect CHD scoring and impact breeding decisions. This study aimed to assess the association between pelvic rotation and asymmetry in obturator foramina areas (AOFAs) and to develop a computer vision model for automated AOFA measurement. In the first part, 203 radiographs were analyzed to examine the relationship between pelvic rotation, assessed through asymmetry in iliac wing and obturator foramina widths (AOFWs), and AOFAs. A significant association was found between pelvic rotation and AOFA, with AOFW showing a stronger correlation (R2 = 0.92, p < 0.01). AOFW rotation values were categorized into minimal (n = 71), moderate (n = 41), marked (n = 37), and extreme (n = 54) groups, corresponding to mean AOFA ± standard deviation values of 33.28 ± 27.25, 54.73 ± 27.98, 85.85 ± 41.31, and 160.68 ± 64.20 mm2, respectively. ANOVA and post hoc testing confirmed significant differences in AOFA across these groups (p < 0.01). In part two, the dataset was expanded to 312 images to develop the automated AOFA model, with 80% allocated for training, 10% for validation, and 10% for testing. On the 32 test images, the model achieved high segmentation accuracy (Dice score = 0.96; Intersection over Union = 0.93), closely aligning with examiner measurements. Paired t-tests indicated no significant differences between the examiner and model's outputs (p > 0.05), though the Bland–Altman analysis identified occasional discrepancies. The model demonstrated excellent reliability (ICC = 0.99) with a standard error of 17.18 mm2. A threshold of 50.46 mm2 enabled effective differentiation between acceptable and excessive pelvic rotation. With additional training data, further improvements in precision are expected, enhancing the model's clinical utility. [ABSTRACT FROM AUTHOR]

Simple Summary: Canine mast cell tumors (MCTs) are the more prevalent form of skin tumor in dogs. Variants in a gene named KIT, coding for a transmembrane type III tyrosine kinase receptor, have been identified in these tumors and are likely to be disease-causing. These variants have been found to cause an excessive activation of the c-kit receptor, which, in turn, results in uncontrolled cell proliferation. Knowledge of the KIT variants is useful for understanding the molecular mechanism at the base of the disease, guiding targeted treatment, and for prognostic purposes. While many KIT variants have already been identified in exons 8–11 and 17, the full spectrum of canine MCT variants in both cutaneous and subcutaneous subtypes is not yet complete. Here, we collected 62 cutaneous and subcutaneous MCT samples from 56 dogs from 21 breeds, sequenced the full coding sequence of the KIT gene, and identified its variants. We confirmed previously known variants on exons 8–11 and identified new ones in subcutaneous MCT. Additionally, we confirmed the differential distribution of KIT variants between cutaneous and subcutaneous MCTs. Overall, we expanded the knowledge on the KIT mutational spectrum of these tumor types. Canine mast cell tumors (MCTs) are common skin neoplasms with varying biological behaviors. The KIT proto-oncogene plays a key role in the development of these tumors, and internal tandem duplications on exon 11 are usually associated with more aggressive behavior, increased local recurrence, and decreased survival time. However, apart from exons 8–11 and 17, there is limited understanding of the overall KIT mutational landscape in canine MCTs. This work aims to analyze the entire KIT coding sequence (21 exons) in a cohort of 62 MCTs, which included 38 cutaneous and 24 subcutaneous tumors, and potentially identify new variants. In addition to confirming previously reported activating KIT mutations in exons 8, 9, and 11, we identified new variants in exons 2, 3, 5, 16, and the 3′ untranslated region (UTR). Notably, these last variants include an amino acid change (Asp/His) in exon 16. Additionally, we confirmed a differential prevalence of KIT variants in cutaneous and subcutaneous MCTs. These findings enhance our understanding of the KIT proto-oncogene coding sequence and provide valuable information for future confirmatory studies. [ABSTRACT FROM AUTHOR]

The Korean Sapsaree dog is a native breed known for its distinctive appearance and historical significance in Korean culture. The accurate estimation of breeding values is essential for the genetic improvement and conservation of such indigenous breeds. This study aimed to evaluate the accuracy of breeding values for body height, body length, chest width, hair length, and distraction index (DI) traits in Korean Sapsaree dogs. Additionally, a genome-wide association study (GWAS) was conducted to identify the genomic regions and nearby candidate genes influencing these traits. Phenotypic data were collected from 378 Korean Sapsaree dogs, and of these, 234 individuals were genotyped using the 170k Illumina CanineHD BeadChip. The accuracy of genomic predictions was evaluated using the traditional BLUP method with phenotypes only on genotyped animals (PBLUP-G), another traditional BLUP method using a pedigree-based relationship matrix (PBLUP) for all individuals, a GBLUP method based on a genomic relationship matrix, and a single-step GBLUP (ssGBLUP) method. Heritability estimates for body height, body length, chest width, hair length, and DI were 0.45, 0.39, 0.32, 0.55, and 0.50, respectively. Accuracy values varied across methods, with ranges of 0.22 to 0.31 for PBLUP-G, 0.30 to 0.57 for PBLUP, 0.31 to 0.54 for GBLUP, and 0.39 to 0.67 for ssGBLUP. Through GWAS, 194 genome-wide significant SNPs associated with studied Sapsaree traits were identified. The selection of the most promising candidate genes was based on gene ontology (GO) terms and functions previously identified to influence traits. Notable genes included CCKAR and DCAF16 for body height, PDZRN3 and CNTN1 for body length, TRIM63, KDELR2, and SUPT3H for chest width, RSPO2, EIF3E, PKHD1L1, TRPS1, and EXT1 for hair length, and DDHD1, BMP4, SEMA3C, and FOXP1 for the DI. These findings suggest that significant QTL, combined with functional candidate genes, can be leveraged to improve the genetic quality of the Sapsaree population. This study provides a foundation for more effective breeding strategies aimed at preserving and enhancing the unique traits of this Korean dog breed. [ABSTRACT FROM AUTHOR]

Monitoring mortality rates offers crucial insights into public health by uncovering the hidden impacts of diseases, identifying emerging trends, optimising resource allocation, and informing effective policy decisions. Here, we present a novel approach to analysing premature mortality in companion animals, utilising data from 28,159 deceased dogs and 24,006 deceased cats across the United Kingdom. By employing PetBERT-ICD, an automated large language model (LLM) based International Classification of Disease 11 syndromic classifier, we reveal critical insights into the causes and patterns of premature deaths. Our findings highlight the significant impact of behavioural conditions on premature euthanasia in dogs, particularly in ages one to six. We also identify a 19% increased risk of premature mortality in brachycephalic dog breeds, raising important animal welfare concerns. Our research establishes a strong correlation between socioeconomic status and premature mortality in cats and dogs. Areas with the lowest Index of Multiple Deprivation (IMD) scores show nearly a 50% reduction in the risk of premature mortality across cats and dogs, underscoring the powerful impact that socioeconomic factors can have on pet health and longevity. This research underscores the necessity of examining the socioeconomic disparities affecting animal health outcomes. By addressing these inequities, we can better safeguard the well-being of our companion animals. [ABSTRACT FROM AUTHOR]

Background: DNA methylation is a covalent bond modification that is observed mainly at cytosine bases in the context of CG pairs. DNA methylation patterns reflect the status of individual tissues, such as cell composition, age, and the local environment, in mammals. Genetic factors also impact DNA methylation, and the genetic diversity among various dog breeds provides a valuable platform for exploring this topic. Compared to those in the human genome, studies on the profiling of methylation in the dog genome have been less comprehensive. Results: Our study provides extensive profiling of DNA methylation in the whole blood of three dog breeds using whole-genome bisulfite sequencing. The difference in DNA methylation between breeds was moderate after removing CpGs overlapping with potential genetic variation. However, variance in methylation between individuals was common and often occurred in promoters and CpG islands (CGIs). Moreover, we adopted contextual awareness methodology to characterize DNA primary sequences using natural language processing (NLP). This method could be used to effectively separate unmethylated CGIs from highly methylated CGIs in the sequences that are identified by the conventional criteria. Conclusions: This study presents a comprehensive DNA methylation landscape in the dog blood. Our observations reveal the similar methylation patterns across dog breeds, while CGI regions showed high variations in DNA methylation level between individuals. Our study also highlights the potential of NLP approach for analyzing low-complexity DNA sequences, such as CGIs. [ABSTRACT FROM AUTHOR]

Canine multiple system degeneration (CMSD) is an early onset, progressive movement disorder affecting Kerry Blue Terriers and Chinese Crested dogs. The associated pathologic lesions include degeneration of the cerebellum, caudate nucleus, and substantia nigra. CMSD is inherited as an autosomal recessive trait in both dog breeds. Previous linkage mapping localized the CMSD locus to a 15 MB region on canine chromosome 1 (CFA1). Next-generation sequencing was used to generate whole-genome sequences from the DNA of an affected dog from each breed. The resulting sequence reads were aligned to the NCBI canine reference genome (build 3.1). Among the homozygous sequence variants within the CFA1 target region, a nonsense variant in exon 15 of SERAC1 was identified in the affected Kerry Blue Terrier, while in the Chinese Crested dog, a 4 bp deletion in the SERAC1 exon 4 acceptor splice site was found. RT-PCR showed that this deletion resulted in exon 4 skipping. Genotyping of large cohorts of Kerry Blue Terriers and Chinese Crested dogs for the respective breed-specific SERAC1 variants showed complete concordance between genotype and disease phenotype. Genotype–phenotype concordance was also observed in offspring generated by cross breeding between SERAC1-heterozygous Kerry Blue Terrier and Chinese Crested dogs, with only the compound heterozygotes exhibiting the disease phenotype, further confirming the recessive inheritance of CMSD. Variants in human SERAC1 are associated with disorders with a range of ages of disease onset and patterns of clinical signs, but that are all characterized by movement abnormalities similar to those of the dogs with CMSD. Canine CMSD could serve as a valuable model to elucidate the mechanisms underlying SERAC1-deficiency disorders and to evaluate potential therapeutic interventions. [ABSTRACT FROM AUTHOR]

When classifying breeds of dogs, the accuracy of classification significantly affects breed identification and dog research. Using images to classify dog breeds can improve classification efficiency; however, it is increasingly challenging due to the diversities and similarities among dog breeds. Traditional image classification methods primarily rely on extracting simple geometric features, while current convolutional neural networks (CNNs) are capable of learning high-level semantic features. However, the diversity of dog breeds continues to pose a challenge to classification accuracy. To address this, we developed a model that integrates multiple CNNs with a machine learning method, significantly improving the accuracy of dog images classification. We used the Stanford Dog Dataset, combined image features from four CNN models, filtered the features using principal component analysis (PCA) and gray wolf optimization algorithm (GWO), and then classified the features with support vector machine (SVM). The classification accuracy rate reached 95.24% for 120 breeds and 99.34% for 76 selected breeds, respectively, demonstrating a significant improvement over existing methods using the same Stanford Dog Dataset. It is expected that our proposed method will further serve as a fundamental framework for the accurate classification of a wider range of species. [ABSTRACT FROM AUTHOR]

Background and Aim: Computed tomographic (CT) images can elucidate the variations of cardiac orientation that this information among dog breeds has never been reported. This study aimed to explore the heart orientations of dogs with different thoracic types and study their effects on vertebral heart score (VHS) measurements using CT images. Materials and Methods: Thoracic CT images of 115 mature dogs without thoracic abnormalities were retrospectively examined. The dogs were classified into four groups: Normal Broad, Abnormal Broad, Normal, and Deep. All dogs were also classified based on their heart deviations. The VHSs were evaluated using lateral VHS, dorsal VHS, and adjusted VHS, and all were compared. Results: In the normal broad and abnormal broad groups, the lateral VHS and lateral long-axis dimensions were significantly lower than those obtained from the dorsal and adjusted VHSs. In addition, heart deviations were mostly observed in the normal broad and abnormal broad groups. Nevertheless, little evidence was found in the normal and deep groups. The lateral VHS and lateral long-axis dimensions were significantly reduced by heart deviation more than the dorsal and adjusted VHSs. Conclusion: Cardiac orientations among dog breeds can affect VHSs of lateral projection, especially in the broad thoracic group. Clinical evaluation of the VHS in the broad thoracic dogs should be performed on the dorsal view for more accurate measurement of heart size. [ABSTRACT FROM AUTHOR]

Importance: The composition of the gut microbiota is essential for a dog’s health and its adaptation to the environment. Different bacteria can produce the same essential metabolites beneficial to health owing to bacterial functional redundancy in microbial communities. Objective: This study examined the gut bacterial communities of dogs from different breeds, all kept under identical domestication conditions. Methods: Noninvasive sampling and 16S rRNA high-throughput sequencing were used to compare the composition and function of the gut microbiota of three dog breeds: the Chinese Kunming dog (CKD), German Shepherd dog (GSD), and Belgian Malinois dog (BMD). Results: The gut microbiota of the three dog breeds consisted of 257 species across 146 genera, 60 families, 35 orders, 15 classes, and 10 phyla. The dominant bacterial phyla across the three breeds were Firmicutes (57.44%), Fusobacteriota (28.86%), and Bacteroidota (7.63%), while the dominant bacterial genera across the three breeds were Peptostreptococcus (21.08%), Fusobacterium (18.50%), Lactobacillus (12.37%), and Cetobacter (10.29%). Further analysis revealed significant differences in the intestinal flora of the three breeds at the phylum and genus levels. The intestinal flora of BMD was significantly richer than that of CKD and GSD. The functional prediction and Kyoto Encyclopedia of Genes and Genomes analysis showed that the primary functions of the gut microbiota in these breeds were similar, with significant enrichment in various metabolic pathways, including carbohydrate and amino acid metabolism, secondary metabolite biosynthesis, and microbial metabolism in different environments. The intestinal flora of these breeds also played a crucial role in genetic information processing, including transcription, translation, replication, and material transport. Conclusions and Relevance: These results provide novel insights into the intestinal flora of intervention dogs and suggest novel methods to improve their health status, which help increase microbial diversity and normalize metabolite production in diseased dogs. [ABSTRACT FROM AUTHOR]

• Developmental validation of novel qPCR multiplex for canine DNA quantification. • Canine DNA successfully recovered and quantified from attacked livestock. • Methods reveal limitations of current commercial canine STR kit. • Clear steps for improvements in canine forensic DNA pipeline identified and discussed. The absence of a standardised method to quantify canine DNA recovered from livestock attacks leaves forensic providers without an important quality control step to help support their decision making. Typically used to normalise the amount of DNA for STR amplification, modern forensic DNA quantification approaches use qPCR of target genes and can also include an Internal Positive Controls (IPC) to determine the presence of PCR inhibitors. The co-amplification of livestock DNA alongside canine DNA has meant that previously developed qPCR methods are not suitable for use so a standardised approach is needed. This research describes the development of a Taq-man multiplex qPCR assay that simultaneously quantifies the autosomal MC1R and Y-specific SRY gene to determine the concentration of canine DNA recovered from attacked livestock. Data suggests that the method is robust and reproducible with no significant difference in the standard curves produced from multiple runs or from different DNA standards derived from different canines. Assay sensitivity of between 15 and 31 pg is consistent with other forensic quantification assays and also in line with the sensitivity of the two tested canine STR kits, Canine Genotype 2.1 Kit and CaDNAP Panels 1 and 2. The assay is highly specific to canines when tested against 163 different dogs representing 33 different breeds and no cross-amplification of non-target species' DNA was observed even from livestock DNA tested at 31.25 ng/µl. This strongly suggests that any DNA detected on evidence collected from attacked livestock is canine. The assay also shows robust tolerance to common livestock inhibitors continuing to amplify when inhibitor-spiked DNA samples were tested. Both mixed and inhibited DNA samples underwent STR typing using two canine forensic STR kits with data showing the Canine Genotype 2.1 Kit displaying pronounced cross-amplification of livestock DNA and and/or extensive PCR inhibition leading to the complete loss of amplification when using this kit. Conversely the CaDNAP Panels 1 and 2 showed little cross-amplification of livestock DNA and improved inhibitor tolerance suggesting that this approach was better suited for the analysis of livestock attack samples. Findings are discussed and the impact of the observations on future work in this area are explored. [ABSTRACT FROM AUTHOR]

Abstract Most studies on dogs’ olfactory performance focused only on some individuals pre-trained for a task or on specially selected/trained detection dogs. Here, using the Natural Detection Task (NDT) that does not require training, we assessed the effect of several potential factors on the performance of a large sample of dogs (N = 527/tested, 484/analysed). Olfactory success was associated with breeds, but breed groups (selected for olfaction, cooperation, or both) were proven non-relevant, suggesting that breed-specific traits are more influential than functional breed group characteristics. Border collies, selected for herding, reached higher success levels than golden retrievers, Hungarian/German vizslas and basset/bloodhounds, selected for hunting/olfaction tasks. Beagles found the hidden food quicker than border collies, basset/bloodhounds, golden retrievers, Labradors, and cocker spaniels. Dogs with higher Responsiveness to training personality scores were more successful, while higher training level linked to slower successful search. Activity/Excitability scores, attention-deficit/hyperactivity disorder scores, and owners’ rewarding style were not associated with performance. Overall, large within-breed/breed-group variabilities reflect complex interactions between genetic and environmental factors influencing performance. Given the complex nature of life-like detection tasks, the NDT may provide more information about dogs’ olfactory ability than on their potential effectiveness in detection work.

Cancer is a leading cause of death in domestic dogs. Deaths due to cancer vary widely among breeds, providing an opportunity for testing the multi-stage model of carcinogenesis. This model underpins evolutionary and basic studies of cancer suppression and predicts a linear increase in cancer with breed size, an expectation complicated by bigger breeds having a shorter lifespan (decreasing risk). Using three independent datasets, the weight and lifespan of breeds provided a good fit of lifetime cancer mortality to the multi-stage model, the fit suggesting many canine cancers are initiated by four driver mutations. Of 85 breeds in more than one dataset, only flat-coated retriever showed significantly elevated cancer mortality, with Scottish terrier, Bernese mountain dog and bullmastiff also showing notable risk (greater than 50% over expected). Analysis of breed clades suggested terriers experience elevated cancer mortality. There was no evidence that the lower mass-specific metabolic rate of larger breeds reduced cancer risk. Residuals indicated increased breed inbreeding shortened expected lifespan, but had no overall effect on cancer mortality. The results provide a baseline for identifying increased breed risk for specific cancers and demonstrate that, unless selection promotes increased cancer suppression, the evolution of larger longer-lived animals leads to a predictable increased cancer risk.

Background: While complex dog–human coexistence has been deeply investigated, there is a relative scarcity of similar knowledge regarding dog–dog interactions. Social learning, a fundamental synchronizing mechanism between dogs and humans, was recently found to be influenced by the functional breed selection of dogs: with the cooperative breeds being more effective learners from a human demonstrator than the independent working breeds were. Here, we investigated whether these differences would also be present when dogs had to learn from another dog and how to effectively perform a detour around a transparent V-shaped obstacle. We tested dogs from 28 independent and 19 cooperative breeds in three consecutive trials. In the control groups, all dogs had to detour on their own the obstacle. In the dog demonstration groups, in trial 1, the subjects had to detour on their own, but before the next two trials, a trained dog showed them the solution. Results: We found that the performance of the two breed groups was the same in the without demonstration groups. However, after observing the dog demonstrator, the independent dogs learned the task more successfully than the cooperative breeds did. In the case of the independent working breeds, detour latencies significantly dropped along the consecutive trials, and these dogs also showed higher rate of successful detours after observing the demonstrator dog's action than in the control group. Conclusions: This is the first study where the consequences of functional breed selection were confirmed in a scenario that involved conspecific social learning in dogs. The results fit well to the ecologically valid framework of the evolutionary past of dog breed formation, in which cooperative breeds were selected for their interactivity with humans, whereas independent breeds often had to work together with their conspecifics. [ABSTRACT FROM AUTHOR]

The Kazakh Tobet is an indigenous Kazakh dog breed that has been used to guard livestock since ancient times. To understand the genetic structure and phylogenetic relationship of the Kazakh Tobet breed with other herding and livestock guarding dog breeds, we analysed short tandem repeat data of 107 Kazakh Tobet dogs from different regions of Kazakhstan and Mongolia, as well as whole genome sequencing data from two Kazakh Tobet dogs and 43 dogs from 24 working breeds. Our results indicate a high genetic diversity of the Kazakh Tobet, with the average number of alleles per locus ranging from 6.00 to 10.22 and observed heterozygosity ranging from 76 to 78%. The breed has a complex genetic structure characterised by seven different clusters. The neighbour-joining tree constructed based on 14,668,406 autosomal and the maximum likelihood tree based on mitochondrial D-loop sequences indicate a common genetic heritage between the Kazakh Tobet, the Central Asian Shepherd Dog and the Turkish Akbash. The presence of haplotype A18 in the Kazakh Tobets supports the hypothesis of the ancient origin of the breed, which was previously suggested by archaeological finds and written sources. These results provide an important genetic basis for the ongoing efforts to improve the Kazakh Tobet breed, to ensure its preservation as an independent genetic lineage and to recognise a breed on an international level. [ABSTRACT FROM AUTHOR]

Objective: to analyze Brazilian trends regarding kinetherapy as a therapeutic intervention for the health of adults and elderly people. Method: narrative review study, carried out on the theses and dissertations portal of the Coordination for the Improvement of Higher Education Personnel and on the Brazilian Digital Library of Theses and Dissertations. Narrative and descriptive analysis was carried out. Results: there was a predominance of dissertations, with a qualitative approach, produced by the areas of speech therapy and psychology. No homogeneity was identified in the aspects involving dog therapy (dog breed and number, duration and intervals of sessions). The dog acts as a facilitator of interactions and the establishment of emotional bonds. Kinotherapy can provide relief from stress, tension and depressive symptoms. Conclusion: national trends in kinetherapy were identified as a therapeutic intervention to promote and recover the health of adults and elderly people. This is a growing practice in healthcare. [ABSTRACT FROM AUTHOR]

Kennel club registrations have been used in a number of studies to estimate ownership levels and fashion trends among dog breeds. They cannot, however, give a complete overview of the contemporary trade in pet dogs, and additionally, may be particularly poor at monitoring trends among some welfare-vulnerable breeds. Here, we compared data from online advertisements for the UK sales of single-breed dogs over an 18-month period, with equivalent data (including for prior and subsequent years) from the UK Kennel Club. Kennel Club registration rates and breed popularity rates online were positively correlated. However, some breeds, including the Jack Russell terrier, Border collie and Chihuahua were considerably over-represented in online advertisements when compared with Kennel Club registrations, indicating a hidden market for certain breeds. In addition, the online dataset provided information on the sale and re-homing of adult dogs, showing high rates of adult homing of the Staffordshire bull terrier, Siberian husky and Rottweiler. We conclude that online pet-sales websites are an important and complementary source of data about the market in, and popularity of, a wide range of dog breeds. [ABSTRACT FROM AUTHOR]

Dilated cardiomyopathy (DCM) is a major cause of morbidity and mortality in various dog breeds, being the second most common acquired cardiac disease in dogs. The most frequently affected breeds are Doberman Pinchers, Great Danes, Boxers, Irish Wolfhounds and others. We found out that the Weimaraner breed also suffers from this disease. Prospective dog screening may identify animals with DCM at Stage B. In the study, a total of 331 dogs of the Weimaraner breed were enrolled. Of the total number, 300 dogs were healthy. Seventeen dogs were diagnosed with preclinical DCM. The cut-off value for preclinical DCM of NTproBNP (N-terminal prohormone of brain natriuretic peptide) was 405 pmol/l, sensitivity was 73% and specificity was 72%. The cut-off value for preclinical DCM of troponin I was 0.04 ng/ml, sensitivity was 66% and specificity was 88%. This is the first study to evaluate the use of NTproBNP and cTnI as markers of preclinical DCM in Weimaraners. Setting the cut-off values for these parameters shows its clinical validity in detecting the disease, such as the possibility of using these tests in routine clinical practice. [ABSTRACT FROM AUTHOR]

Parkinson's Disease (PD) is a clinically diagnosed disease that carries a reported misdiagnosis rate of 10–20%. Recent scientific discoveries have provided evidence of volatile organic compounds in sebum that are unique to patients with PD. The primary objective of this study was to determine if companion dogs could be trained to distinguish between sebum samples provided by PD-positive patients and PD-negative human controls. This was a randomized, handler-blind, controlled study. Twenty-three canines of varying breeds, ages, and environmental backgrounds were included. The study period encompassed 200 total working days from 2021 to 2022. Factors investigated included donor gender and levodopa drug affectivity, as well as canine breed, age, and duration of training time. The findings in this study were compiled from data collected during the final two years of a seven-year research program. For this two-year reporting period, when averaged as a group, the 23 dogs were 89% sensitive and 87% specific to olfactory distinction between PD-positive and PD-negative human donor samples. Ten of the twenty-three dogs averaged 90% or higher in both sensitivity and specificity. In 161 separate trials, a dog was presented with both novel PD-positive and PD-negative samples. For these novel exposures, the dogs collectively averaged 86% sensitivity and 89% specificity. PD medication was also investigated and was found to have no discernible impact on canine sensitivity or specificity results. Study findings support the application of companion dogs, trained with force-free, reward-based methodologies, for the detection of PD-positive and PD-negative samples under controlled conditions. [ABSTRACT FROM AUTHOR]

Objective: To evaluate the prevalence, type, site and breed predisposition of thoracic congenital vertebral malformations (CVM) in neurologically normal brachycephalic 'screw‐tail' dog breeds. Study Design: Retrospective case series. Animals: Neurologically normal French Bulldogs (n = 63), British Bulldogs (n = 42), Boston Terriers (n = 4) and Pugs (n = 86) presenting for brachycephalic obstructive airway syndrome treatment. Methods: Either a plain computer tomography scan of the thorax or lateral and dorso‐ventral digital radiographs of the thoracic vertebral column of each dog were assessed for any vertebral malformations and classed according to the Modified McMaster vertebral abnormality classification scheme proposed by Guiterrez et al. Results: The prevalence of dogs with one or more abnormal vertebrae was 96.82% in French Bulldogs, 90.47% in British Bulldogs, and 76.74% in Pugs. Pugs had significantly lower instances of CVMs than French Bulldogs. The four Boston Terriers all had vertebral malformations, but the results were not considered to be statistically significant due to their small sample size. In French Bulldogs and British Bulldogs, ventral hypoplasia (Type 3) occurred at greater rates than all other types, followed by ventral and median hypoplasia (Types 7) and symmetrical hypoplasia (Type 8). In Pugs, symmetrical hypoplasia (Type 8) was the most common and then ventral hypoplasia (Type 3). The most affected vertebrae were T6–T9 in Pugs and T5–T10 in French Bulldogs and British Bulldogs. Conclusions: The majority of neurologically normal brachycephalic 'screw‐tail' dogs assessed in this paper possessed one or more congenital thoracic vertebral malformations. Dogs with vertebral malformations were found to be more likely to have more than one affected vertebra. Sex had no effect on the prevalence of CVM. Clinical Relevance: A substantial number of neurologically normal brachycephalic 'screw‐tail' dogs suffer from congenital vertebral malformations. Thus, the need for selective breeding programmes to eliminate this hereditary condition is crucial. [ABSTRACT FROM AUTHOR]

ABSTRACT Large dog breeds commonly produce unformed faeces. The present study hypothesised that foods for large dog breeds require higher starch gelatinisation (SG) to reduce organic matter flow to colon. Fifteen Rottweilers (Ro; 49.4 ± 6.12 kg), 18 Beagles (Be; 12.13 ± 1.75 kg) and 20 Shih‐Tzus (ST; 4.62 ± 1.15 kg) were fed one of three diets, all based on the same sorghum formulation, processed to obtain three levels of SG: SG90 (91.8% SG), SG50 (50.7% SG) and SG30 (27.4% SG). Foods were provided for 23 days, and the coefficient of total tract apparent digestibility (CTTAD) of nutrients, faecal production and fermentation products, Na, K and Cl apparent absorption and gastrointestinal transit time (GTT) were evaluated. Results were submitted to analysis of variance considering SG, breed and their interactions, and means compared by the Tukey test (p < 0.05). All dogs promptly ate the foods. Faeces scores were lower for Ro than for Be and ST, and lower for SG30 than SG90 (p < 0.05). Faeces pH was higher, and total short‐chain fatty acids were lower for SG90 than for SG50 and SG30 (p < 0.01), regardless of breed. No diet effect was observed for GTT (p > 0.05), but it was lower for Ro (41.7 ± 6.2 h) than for ST (48.7 ± 8.6 h). The CTTAD of nutrients was lower for SG30, intermediary for SG50 and higher for SG90 (p < 0.05), and among breeds CTTAD was higher for Be than ST and Ro (p < 0.05), which did not differ from each other, except for crude protein CTTAD which was lower for Ro (p < 0.05). The apparent absorption of Na and K was higher for the SG90 treatment, and for Be in comparison with Ro and ST (p < 0.05). Food SG had a remarkable influence on Ro faeces formation and on the CTTAD in all breeds. Greater fermentation in the colon and lower protein CTTAD may be involved in Ro unformed faeces formation. [ABSTRACT FROM AUTHOR]

Three-dimensional (3D)-printed models of bones are a convenient and durable alternative to real bone specimens, and they have been used in anatomy laboratories. It is necessary to identify the precise advantages of 3D-printed models from all perspectives; not only the improvement in students' knowledge of anatomy but also the students' assessment of such models. Here, students of veterinary medicine and animal science evaluated the reproducibility and effectiveness of 3D-printed models as a learning tool by completing our questionnaires, with a focus on their understanding of the skull-morphological differences among dog breeds. With the COVID-19 pandemic having obliged veterinary universities to provide courses online, we also investigated how the pandemic affected the students' evaluation of the 3D-printed models. The questionnaire results revealed that the animal science students were satisfied with the reproducibility of the 3D-printed models, but the veterinary students were not (they preferred to use real specimens). The skull differences were well understood by both types of students, indicating that 3D-printed models are effective for learning about rare skeletal specimens. The veterinary students who experienced the COVID-19 pandemic tended to choose real specimens more often than those who did not have this experience. Our results suggest that the use of 3D-printed models as an introduction and the use of real specimens in anatomy laboratory courses can be adequate for veterinary students. Together our findings suggest ways to improve the educational performance of 3D-printed models for veterinary students who need to understand the anatomy of many species. [ABSTRACT FROM AUTHOR]

Simple Summary: This study focused on a 10-year-old dog presenting with weight loss, excessive drinking, and liver-related issues. The aim was to understand a rare type of lymphoma affecting the liver and spleen, known as hepatosplenic T-cell lymphoma. By examining tissue samples from the spleen, liver, and gallbladder, researchers identified abnormal cells indicating hepatosplenic T-cell lymphoma. Surgery and subsequent treatment with toceranib, a drug not commonly used for this lymphoma in dogs, led to a notable improvement in the dog's condition. This finding suggests toceranib could be an effective treatment option for hepatosplenic T-cell lymphoma in canines. The study's results highlight the potential of new treatments for rare diseases in pets, offering hope for improved care and outcomes in veterinary oncology. Toceranib phosphate (toceranib) is approved for canine mast cell tumor treatment. However, no long-term response to toceranib in canine HSTCL has been reported. Here, we describe a case of a 10-year-old castrated mixed-breed dog that presented with a 3-month history of weight loss, polydipsia, and polyuria. The clinicopathological and imaging abnormalities included icterus, biliary obstruction, and splenomegaly with multiple diffuse splenic hypoechoic nodules. On day 21, a cholecystectomy was performed to remove the obstruction, followed by a liver biopsy and splenectomy. Cytology of the spleen and liver showed many small lymphocytes with intracytoplasmic granules (sGLs). Splenic and hepatic infiltration of neoplastic CD3/granzyme B-positive small cells and lymphocytic cholecystitis with granzyme B-negative small cells were noted. T-cell receptor gene clonal rearrangements were observed in the liver tissues. The dog was diagnosed with a hepatosplenic T-cell lymphoma (HSTCL) of sGLs concurrent with lymphocytic cholecystitis. The icterus resolved after surgery, but there was progressive elevation of liver enzyme levels. Toceranib was administered from day 39, resulting in decreased liver enzyme levels, and the dog remained in good condition. The dog stayed in remission after toceranib administration and survived for 460 days. Toceranib should be considered an effective treatment option for canine HSTCL. [ABSTRACT FROM AUTHOR]