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This article offers a canine history of the "critical period" concept, situating its emergence within a growing, interdisciplinary network of canine behavior studies that connected eugenically minded American veterinarians, behavioral geneticists, and dog lovers with large institutional benefactors. These studies established both logistical and conceptual foundations for large‐scale science with dogs while establishing a lingering interdependence between American dog science and eugenics. The article emphasizes the importance of dogs as subjects of ethological study, particularly in the United States, where some of the earliest organized efforts to analyze canine behavior began. Further, the article argues that the "critical period" is important not only for its lasting prominence in multiple fields of scientific inquiry, but also as a historiographical tool, one that invites reflection on the tendency of historians to emphasize a particular narrative structure of scientific advancement. [ABSTRACT FROM AUTHOR]

A seven-year-old female mongrel dog was presented at the Sokoine University of Agriculture Teaching Animal Hospital for second opinion regarding a progressive swelling of the right hind limb of two months duration. Clinical examination revealed a loss of body condition, tachypnea and tachycardia, a painful immobile solid mass of 20 cm x 14 cm x 10 cm located on the right stifle joint, leucocytosis, and anaemia. Radiographic examination of the joint revealed marked soft tissue swelling with amorphous areas of mineralization and complete destruction of the proximal tibia. Smooth and solid periosteal reaction was seen around the tibia and fibula with a Codman's triangle. Multiple nodules and a mass with soft tissue opacity were seen in the lung fields. Post-mortem examination revealed separation of tibia and fibula, softening of the proximal parts of the tibia, and complete integration of the proximal region of the two bones and articular tissues into the surrounding muscular tissues. Multifocal nodular lesions of variable sizes were mostly found in the lungs and partly in the liver. Histologically, predominantly oval and round cells with variable nuclear sizes and moderate mitoses were observed in tissue samples from the joint, lungs and the liver although some parts of the lung metastases showed both round and spindle shaped tumor cells. Clinical profile is suggestive of aggressive biphasic (spindle and epithelial) type of synovial cell sarcoma with lung and liver metastasis. Early radiographic and biopsy examination of persistent musculoskeletal nodules is recommended for early diagnosis and interventions. [ABSTRACT FROM AUTHOR]

The article describes the case of a neutered male Golden retriever service dog approximately 5 years of age presented with a sudden refusal to enter certain public venues, with a history of attempts to escape followed by barking, panting, trembling and pacing when left at home, during thunderstorms or at the sound of distant gunshots. Diagnosis include noise-related anxiety and noise phobia. Communication through a cure-response-reward system and psychotropic medications were prescribed.

Few veterinary studies have evaluated the response to chemotherapy treatment of canine intranasal tumours, while many have focused on the efficacy of radiation therapy. Given the higher costs and limited access to radiation therapy, alternative treatment options are needed. The study describes a cohort of dogs with histologically confirmed intranasal tumours treated with chemotherapy as a sole therapy. This retrospective study was conducted using data from the Melbourne Veterinary Specialist Centre (MVSC) database between 2004 and 2017. Dogs with a histologically confirmed intranasal tumour who received chemotherapy treatment were included. Signalment, presenting signs, tumour type, chemotherapy details, adverse events (AEs) and survival times were reviewed. Twenty‐nine dogs met the inclusion criteria. Overall median survival time for dogs in the study was 234 days (range 12‐1698 days). Median survival for dogs with adenocarcinoma or carcinoma (n = 12) was 280 days, transitional cell carcinoma (n = 6) 163 days, squamous cell carcinoma, anaplastic carcinoma or undifferentiated carcinoma (n = 7) 59 days and all sarcomas (n = 4) 448 days. Adverse events were reported following 28% of treatments and 69% of dogs experienced at least one AE. Twenty four per cent of all dogs experienced grade 3 or 4 toxicities. The chemotherapy protocol was generally well tolerated. The study suggests potential benefit in the use of chemotherapy for dogs with adenocarcinoma, carcinoma and sarcoma. [ABSTRACT FROM AUTHOR]

Visceral leishmaniasis (VL) is a life-threatening outcome of Leishmania infantum or Leishmania donovani infection. Dogs are the primary domestic reservoir of L. infantum parasites, and ownership of infected dogs increases the risk of human VL. Controlling infection within dog populations is regarded as critical to VL management in endemic countries, both preventing progression of canine disease and limiting parasite transmission to humans and dogs. Here we discuss various strategies that are used to diagnose canine visceral leishmaniasis (CVL) and the possibilities of adapting these for use within population screening and control programs. In addition, given the variable transmissibility of L. infantum to the sand fly vector, we outline some possibilities for the preferential identification of 'super-spreader' dogs among the overall infected population. Highlights VL is an important disease of dogs and humans, with dogs being the primary domestic reservoir of L. infantum parasites. Detection of diseased, infected, and parasite-transmitting 'super-spreader' dogs is likely critical for control. Dependent upon scale, direct and indirect detection methods can be used for surveillance. Novel molecular assays with point-of-care potential are emerging. New antigen targets are being characterized to enhance performance of serological assays. Adaptation to provide point-of-care serological tests to permit Leishmania detection and facilitate large-scale screening programs. [ABSTRACT FROM AUTHOR]

Over the past two decades, animal-assisted interventions (AAIs), defined as the purposeful incorporation of specially trained animals in services to improve human health, have become increasingly popular in clinical settings. However, to date, there have been few rigorously-designed studies aimed at examining the impact of AAIs on therapy animals, despite a notable potential for stress. The current study measured physiological and behavioral stress indicators in therapy dogs who participated in AAI sessions in pediatric oncology settings, while also examining the psychosocial effects for patients and their parents. This manuscript describes the study’s canine stress findings. A total of 26 therapy dog-handler teams were paired with newly diagnosed children with cancer at five children’s hospitals in the United States. These teams provided regular AAI visits to the child and his/her parent(s) for a period of four months. The teams completed a demographic form, the Canine Behavioral Assessment & Research Questionnaire (C-BARQ), and a self-report survey to document the types of activities that occurred during each session. Canine saliva was also collected at five baseline time points and 20 min after the start of study sessions for cortisol analysis, and all study sessions were video recorded to document the dog’s behavior via an ethogram measure. Data showed no significant differences in salivary cortisol levels between baseline (0.51 μg/dL) and AAI sessions (0.44 μg/dL; p = 0.757). Higher salivary cortisol was significantly associated with a higher number of stress behaviors per session (p = 0.039). There was a significant relationship between stress and affiliative session behaviors (p < 0.0001), indicating that dogs who exhibited more stress behaviors also exhibited more affiliative behaviors. The dog’s most commonly coded session behaviors were oral behaviors, such as lip licking, and tail wagging. The only C-BARQ factor that was found to have a significant association was stranger-directed fear; higher scores on this factor were significantly associated with the dog exhibiting fewer affiliative behaviors in sessions (b = 2.12, p = 0.042). Results show that therapy dogs did not have significantly increased physiological stress responses, nor did they exhibit significantly more stress-related behaviors than affiliative-related behaviors, while participating in AAIs in pediatric oncology settings. There was a significant relationship between canine cortisol and behavior, thus strengthening the argument for the use of cortisol in canine well-being research. This study discusses the importance of further investigation to confirm these findings, and to enhance therapy dog involvement in hospital settings. [ABSTRACT FROM AUTHOR]

The potential effects of adipose tissue-derived mesenchymal stem cells (AT-MSCs) on the growth and invasion of canine tumours including hepatocellular carcinoma (HCC) are not yet understood. Moreover in humans, the functional contribution of AT-MSCs to malignancies remains controversial. The purpose of this study was to investigate the effects of AT-MSCs on the proliferation and invasion of canine HCC cells in vitro. The effect of AT-MSCs on mRNA levels of factors related to HCC progression were also evaluated. Conditioned medium from AT-MSCs (AT-MSC-CM) significantly enhanced canine HCC cell proliferation and invasion. Moreover, mRNA expression levels of transforming growth factor-beta 1, epidermal growth factor A, hepatocyte growth factor, platelet-derived growth factor-beta, vascular endothelial growth factor, and insulin-like growth factor 2 were 2.3 ± 0.4, 2.0 ± 0.5, 5.7 ± 1.9, 1.7 ± 0.2, 2.1 ± 0.4, and 1.4 ± 0.3 times higher, respectively (P < 0.05). The mRNA expression level of MMP-2 also increased (to 4.0 ± 1.2 times control levels) in canine HCC cells co-cultured with AT-MSCs, but MMP-9 mRNA significantly decreased (to 0.5 ± 0.1 times control levels). These findings suggest that soluble factors from AT-MSCs promote the proliferation and invasion of canine HCC cells. [ABSTRACT FROM AUTHOR]

Osteosarcoma (OSA) is a rare cancer in people. However OSA incidence rates in dogs are 27 times higher than in people. Prognosis in both species is relatively poor, with 5 year OSA survival rates in people not having improved in decades. For dogs, 1 year survival rates are only around ~ 45%. Improved and novel treatment regimens are urgently required to improve survival in both humans and dogs with OSA. Utilising information from genetic studies could assist in this in both species, with the higher incidence rates in dogs contributing to the dog population being a good model of human disease. This review compares the clinical characteristics, gross morphology and histopathology, aetiology, epidemiology, and genetics of canine and human OSA. Finally, the current position of canine OSA genetic research is discussed and areas for additional work within the canine population are identified. [ABSTRACT FROM AUTHOR]

Background: Rhipicephalus sanguineus is the most widely distributed tick in the world, which is partly due to its biological flexibility and the global distribution of its major host, the domestic dog. In Mediterranean region it could be principal reservoir host for Leishmania infantum, usually transmitted by the phlebotomine sand flies. In this study, we evaluated the vector potential of R. sanguineus in transmitting L. infantum to uninfected dogs. Methods: During 2014, five dogs with clinical manifestations of canine visceral leishmaniasis (CVL), high anti-Leishmania antibody titers and tick infestation, were selected from CVL endemic areas (Tehran and Alborz provinces). At least, twenty live ticks were removed from each infected dog. After morphological identification, the ticks were divided into two groups; ticks belonging to the first group were dissected for parasitological examinations and semi-nested PCR assay, and those of the second group were selected for the transmission of CVL caused by L. infantum to uninfected dogs. Following tick infestation, all uninfected dogs were kept for 9 months and examined monthly for clinical and serological tests. Results: Nearly, 67% of ticks were infected by L. infantum using the semi-nested PCR. All other parasitological tests of ticks were negative. Clinical examinations and serological tests of the investigated dogs revealed negative results. Nested-PCR test results performed on splenic biopsy samples of dogs were also negative. Conclusion: L. infantum-positive R. sanguineus ticks were unable to transfer L. infantum from infected dogs to healthy ones. The detection of L. infantum DNA in ticks collected from naturally infected dogs by semi-nested PCR does not prove their vectorial competence. [ABSTRACT FROM AUTHOR]

Canine resource guarding (RG) describes the behaviour used by a dog to achieve or maintain control over an item of perceived value. Three distinct behavioural patterns of RG have been proposed; rapid ingestion (i.e., rapid consumption of an edible item), avoidance (i.e., positioning of the head or body to maintain item control, or location change with the item) and aggression. Research and clinical treatment has been mainly focused on aggressive forms due to the potential for harm to people and other animals. However, rapid ingestion and avoidance patterns are equally important since they may be precursors to aggression, with owner response being an important influencing factor. If this hypothesis is correct, accurate owner recognition of all patterns of RG behaviour is an important first step in preventing future aggression around items. The aim of this study was to determine if owners were able to accurately identify types of RG behaviour patterns, and if additional dog experience influences this ability. Owners (n = 1438) were recruited to watch videos, each involving different examples of RG behaviour patterns, and asked to identify each of the categories they observed. Four canine behaviour experts independently validated the videos, prior to the study, for the type of behaviour pattern(s) displayed. Data were analysed using multi-level logistic regression in Stata 13. Owners were more likely to correctly identify the presence or absence of biting aggression compared to all other RG behaviour patterns (p < 0.001). Owners were significantly more likely to correctly identify the absence of a behaviour pattern (i.e., rule out the pattern's existence) compared to the presence of a behaviour pattern (p < 0.001). Owners that reported having an advanced knowledge of dog behaviour (p < 0.01) or had participated in professional dog training classes (p < 0.01) were significantly better at correctly identifying RG behaviour patterns. The results suggest owners are good at identifying obvious RG aggression but could improve their ability to recognize other patterns of RG. Future research is needed to examine whether identification can be improved through alternative methods of training. Future efforts could aim to educate owners on appropriate methods to prevent escalation of RG behaviour when it is observed. [ABSTRACT FROM AUTHOR]

Background: Fear/anxiety and anger/aggression greatly influence health, quality of life and social interactions. They are a huge burden to wellbeing, and personal and public economics. However, while much is known about the physiology and neuroanatomy of such emotions, little is known about their genetics - most importantly, why some individuals are more susceptible to pathology under stress. Results: We conducted genomewide association (GWA) mapping of breed stereotypes for many fear and aggression traits across several hundred dogs from diverse breeds. We confirmed those findings using GWA in a second cohort of partially overlapping breeds. Lastly, we used the validated loci to create a model that effectively predicted fear and aggression stereotypes in a third group of dog breeds that were not involved in the mapping studies. We found that i) known IGF1 and HMGA2 loci variants for small body size are associated with separation anxiety, touch-sensitivity, owner directed aggression and dog rivalry; and ii) two loci, between GNAT3 and CD36 on chr18, and near IGSF1 on chrX, are associated with several traits, including touch-sensitivity, non-social fear, and fear and aggression that are directed toward unfamiliar dogs and humans. All four genome loci are among the most highly evolutionarily-selected in dogs, and each of those was previously shown to be associated with morphological traits. We propose that the IGF1 and HMGA2 loci are candidates for identical variation being associated with both behavior and morphology. In contrast, we show that the GNAT3-CD36 locus has distinct variants for behavior and morphology. The chrX region is a special case due to its extensive linkage disequilibrium (LD). Our evidence strongly suggests that sociability (which we propose is associated with HS6ST2) and fear/ aggression are two distinct GWA loci within this LD block on chrX, but there is almost perfect LD between the peaks for fear/aggression and animal size. Conclusions: We have mapped many canine fear and aggression traits to single haplotypes at the GNAT3-CD36 and IGSF1 loci. CD36 is widely expressed, but areas of the amygdala and hypothalamus are among the brain regions with highest enrichment; and CD36-knockout mice are known to have significantly increased anxiety and aggression. Both of the other genes have very high tissue-specificity and are very abundantly expressed in brain regions that comprise the core anatomy of fear and aggression - the amygdala to hypothalamic-pituitary-adrenal (HPA) axis. We propose that reduced-fear variants at these loci may have been involved in the domestication process. [ABSTRACT FROM AUTHOR]

Excessive dog barking is among the leading sources of noise pollution worldwide; however, the reasons for the annoyance of barking to people remained un-investigated. Our questions were: is the annoyance rating affected by the acoustic parameters of barks; does the attributed inner state of the dog and the nuisance caused by its barks correlate; does the gender and country of origin affect the subjects’ sensitivity to barking. Participants from Hungary (N = 100) and Brazil (N = 60) were tested with sets of 27 artificial bark sequences. Subjects rated each bark according to the inner state of the dog and the annoyance caused by the particular bark. Subjects from both countries found high-pitched barks the most annoying: however, harsh, fast-pulsing, low-pitched barks were also unpleasant. Men found high-pitched barks more annoying than the women did. Annoyance ratings showed positive correlation with assumed negative inner states of the dog, positive emotional ratings showed negative correlation with the annoyance level. This is the first indication that acoustic features that were selected for effective vocal signalling may be annoying for human listeners. Among the explanations for this effect the role of affective communication and similar bioacoustics of particular animal vocalizations and baby cries are discussed. [ABSTRACT FROM AUTHOR]

The article describes the case of a 2-year-old spayed female Border Collie examined because it was frequently chasing lights and shadows and spinning in circles. Topics covered include the behavior and health history of the dog, the findings of physical and laboratory examinations which led to the diagnosis of vitreous degeneration and myodesopsia, and patient outcome following a behavior modification program and administration of antiseizure medications.

To investigate the stress distribution in a maxillary canine restored with each of four different post systems at different levels of alveolar bone loss. Two-dimensional finite element analysis (FEA) was performed by modeling a severely damaged canine with four different post systems: CAD/CAM zirconia, CAD/CAM glass fiber, cast titanium, and cast gold. A force of 100 N was applied to the crown, and the von Mises stresses were obtained. FEA revealed that the CAD/CAM zirconia post system produced the lowest maximum von Mises stress in the dentin layer at 115.8 MPa, while the CAD/CAM glass fiber post produced the highest stress in the dentin at 518.2 MPa. For a severely damaged anterior tooth, a zirconia post system is the best choice while a cast gold post ranks second. The CAD/CAM glass fiber post is least recommended in terms of stress level in the dentin. [ABSTRACT FROM AUTHOR]

Objective--To determine changes in Canine Brief Pain Inventory scores for dogs with osteoarthritis after administration of a monoclonal antibody (mAb) against nerve growth factor (NGF) that was modified by use of a proprietary process for administration to dogs. Animals--11 adult dogs. Procedures--Dogs received the anti-NGF mAb (0.2 mg/kg, IV) at various evaluation times during the study period; at other evaluation times, dogs received an equivalent volume of PBS solution IV. Owners determined Canine Brief Pain Inventory pain severity (PS) and pain interference (PI) scores immediately before (baseline) and 2, 4, and 6 weeks after administration of the anti-NGF mAb; owners were unaware of the evaluation time at which the mAb had been administered. Results--Compared with baseline PS scores (median, 4.75; range, 0.75 to 8.5), dogs had significantly lower PS scores 2 weeks (median, 3; range, 1 to 5.5) and 4 weeks (median, 2.25; range, 0.25 to 725) after administration of anti-NGF mAb. Compared with baseline Pi scores (median, 5.33; range, 1.17 to 9.33), dogs had significantly lower PI scores 2 weeks (median, 3; range, 0.67 to 6.83) and 4 weeks (median, 3.33; range, 0.67 to 6.67) after administration of anti-NGF mAb. The PS and PI scores 6 weeks after mAb administration were lower than baseline scores, although values were not significantly different. Conclusions and Clinical Relevance--Results of this study suggested the evaluated anti-NGF mAb decreased PS and PI scores for 4 weeks after administration. This treatment may be effective for alleviation of signs of pain in dogs with osteoarthritis for up to 4 weeks. [ABSTRACT FROM AUTHOR]

The geriatric canine is an increasing population where breed type determines the aged patient. Multi-medical problems exist and must be considered in any physiotherapy programme prescribed. Close attention should be paid to pain, and pain eliminated wherever possible to achieve better outcomes so that the therapist can achieve the functional and realistic goals being set. This article outlines the potential physical problems presented in the elderly canine and the associated physiotherapy that can be used to address these. The aim of the physiotherapy is to reduce pain and improve quality of life. [ABSTRACT FROM AUTHOR]

Simple Summary: This study looked at the reasons why people sought help for their dog's behavior, where those dogs were acquired by the owner, and which breeds of dogs were seen most often. All dogs in this study presented to a veterinary college specialty hospital in the United States. For dogs presenting to the behavioral medicine service, the types of behavior problems and sources of dogs from 1923 cases over twenty years (1997 to 2017) were evaluated. The breeds of dogs presenting for behavior (822) compared to those presenting to the rest of the hospital for non-behavioral reasons (51,052) were compared over ten years (2007 to 2016). The most common behavioral issue was aggression (72.2% of cases). Dogs obtained from breeders decreased and dogs adopted from shelters increased over twenty years. Dogs from the herding and terrier groups presented more often to the behavioral medicine service compared to the rest of the hospital. While these findings are specific to this population, these results could be helpful in making choices about the allocation of resources for the prevention of behavioral problems, such as genetics research or educational support to shelters. Finally, we found that the variations in terminology and available data made comparisons of behavior problems challenging. The purpose of this retrospective case study was to evaluate trends over time in case distribution, sources, and breeds of dogs presenting to the behavioral medicine service at a veterinary college referral hospital in the United States. For case distribution and sources, the available records from the behavior service (n = 1923) from 1997 to 2017 were evaluated. Breeds of dogs presenting to all services (n = 51,052) were compared to behavior cases (n = 822) from 2007 to 2016. Over twenty years, 72.2% of dogs presented for aggression, 20.1% for anxieties/fears/phobias, and 7.4% for miscellaneous behavioral problems. Dogs acquired from breeders decreased and dogs from shelters, rescues, or adopted as a stray increased over twenty years (p < 0.0001). The Herding (p = 0.0124) and Terrier (p < 0.0001) groups were overrepresented for behavior problems as compared to all other services over ten years. Variations in terminology and diagnostic approach made comparisons with earlier studies difficult, which underscores a need for a more consistent methodology in veterinary behavioral medicine. Understanding trends in sources of dogs could direct resources aimed at guiding owners when acquiring a pet dog and preventing behavioral problems. Findings related to breeds could help guide research focused on the genetic contributions to behavior. [ABSTRACT FROM AUTHOR]

Investigators surveyed 30 U.S. military veterans with PTSD who reported having benefited from living with a dog. The subject population included men and women aged 34 to 67, with a mean of 56.9 years (SD = 8.1), who were being treated at two Department of Veterans Affairs (VA) outpatient clinics. Participants received a questioimaire packet designed to assess aspects of their mental and physical health and relationship with a canine companion, which they completed at home and returned either in person or by mail. The packet consisted of the PTSD Checklist-Military. Version (PCL-M); Beck Depression Inventory, Second Edition (BDI-II); Veterans Short Form Health Survey and Health Behaviors Questionnaire (SF-36); Dog Information Sheet; Dog Relationship Questionnaire; and Lexington Attachment to Pets Scale. Respondents indicated that since adopting their dog they had experienced improvement in several areas, including feeling calmer, less lonely, less depressed, and less worried about their and their family's safety. These results suggest that living with a companion dog may help relieve some of the psychological distress associated with PTSD in some veterans. [ABSTRACT FROM AUTHOR]

Abstract: Background: Transumbilical laparoscopy allows the patient to undergo various surgical procedures associated with abdominal disease. The aim of this study was to evaluate the feasibility and safety of transumbilical thoracic exploration and surgical lung biopsy in a canine survival model. Methods: We performed the procedure in 12 dogs weighting 7.1–9.1 kg. The thoracic cavity was accessed using a metal tube inserted via umbilical and diaphragmatic incisions. After transumbilical thoracoscopy, we resected the predetermined lung lobe with an electrocautery loop. We carried out daily clinical examinations, including determination of respiratory rate and rectal temperature. Laboratory parameters (white blood cell count) and inflammatory parameters, including serum interleukin-6 and C-reactive protein, were measured before surgery and at postoperative days 1, 3, 7, and 14. We performed necropsies 2 wk after surgery. Results: We successfully performed corrected surgical lung biopsies for the predetermined lung lobe in all animals, with a median time of 43.5 min (range, 32–65 min). We observed two perioperative complications: One dog had minor postoperative air leakage and one had hemodynamic collapse because of inadequate ventilation. These animals recovered well without signs of perioperative infection. Necropsies at 2 wk after surgery showed no evidence of mediastinitis or peritonitis. Conclusions: Exposure of the thoracic cavity and surgical lung biopsy via a transumbilical incision is feasible in this canine model of survival. This procedure may have potential advantages over currently used transthoracic thoracoscopy techniques. [Copyright &y& Elsevier]

Very few studies have investigated the seasonal aspect of grass pollen sensitisation or its evolution in the allergic canine population. The aim of this study was to evaluate both these aspects. A study of canine grass pollen sensitisation was performed throughout the statistical analysis of 261 intradermal skin testings (IDT=25 allergens tested on average) performed from January 1999 to December 2010. IDTs were performed on dogs with pruritic allergic skin disease. X2 Tests were used for statistical analysis. Two hundred and thirteen dogs (81.6 per cent) were sensitised to at least one allergen, and 56 (21.5 per cent) to at least one grass pollen. No increase in the rate of positive IDT was recorded over three periods: 1999-2002, 2003-2006 and 2007-2010. No statistical correlation was detected between sex, age or birth month and grass sensitisation. Moreover, no link was found between the season in which the IDT was performed and sensitisation, indicating that there is no seasonality to the sensitisation. However, considering the grasses tested over these three periods (grass mix and rye grass), a clear and significant increase in the percentage of dogs sensitised to grass pollen was observed between 1999-2002 and 2007-2010 (14.4 per cent and 27.7 per cent, respectively). The possible reasons for this sensitisation increase of dogs to grass pollen are discussed. [ABSTRACT FROM AUTHOR]

Mutations in RPGRIP1 are associated with early onset retinal degenerations in humans and dogs. Dogs homozygous for a 44 bp insertion including a polyA29 tract potentially leading to premature truncation of the protein, show cone rod degeneration. This is rapid and blinding in a colony of dogs in which the mutation was characterised but in dogs with the same mutation in the pet population there is very variable disease severity and rate of progression. Objective: We hypothesized that this variability must be associated with leakiness of the RPGRIP1 mutation, allowing continued RPGRIP1 production. The study was designed to discover mechanisms that might allow such leakiness. Methods: We analysed alternate start sites and splicing of RPGRIP1 transcripts; variability of polyAn length in the insertion and slippage at polyAn during transcription/translation. Results and Significance: We observed a low rate of use of alternative start codons having potential to allow forms of transcript not including the insertion, with the possibility of encoding truncated functional RPGRIP1 protein isoforms. Complex alternative splicing was observed, but did not increase this potential. Variable polyAn length was confirmed in DNA from different RPGRIP1-/- dogs, yet polyAn variability did not correspond with the clinical phenotypes and no individual was found that carried a polyAn tract capable of encoding an in-frame variant. Remarkably though, in luciferase reporter gene assays, out-of-frame inserts still allowed downstream reporter gene expression at some 40% of the efficiency of inframe controls. This indicates a major role of transcriptional or translational frameshifting in RPGRIP1 expression. The known slippage of reverse transcriptases as well as RNA polymerases and thermostable DNA polymerases on oligoA homopolymers meant that we could not distinguish whether the majority of slippage was transcriptional or translational. This leakiness at the mutation site may allow escape from severe effects of the mutation for some dogs. [ABSTRACT FROM AUTHOR]

Abstract: This article presents a clinical case of predatory aggression that represents a problem for public health. The patient was a 2-year-old male German shepherd referred to the behavior clinic at the School of Veterinary Medicine owing to its aggressive behavior toward people and other dogs. The dog tended to form a pack with the other dogs of the household and killed neighborhood dogs, cats, hens, and rabbits. The presumptive diagnosis was “predatory aggression” associated with “territorial and protective aggression.” The risk analysis determined that the dog should be euthanized. Nevertheless, the owner refused, and hence behavior modification and drug therapy were recommended. A year later, the dog killed again (another dog), indicating that despite the drug and behavioral treatment, it is likely that its predatory aggression will never be resolved. [Copyright &y& Elsevier]

This study compared the prevalence of Sarcocystis neurona canine serum antibodies from dogs with and without a presumptive diagnosis of neurologic disease, and sera from dogs with no accompanying history. Generally, Sarcocystis have a limited host range. However, Sarcocystis neurona causes neurologic disease in many species, including dogs. Major surface antigens displayed by strains of S. neurona may be important in host susceptibility. The results of this study indicate that while S. neurona is an uncommon infection in dogs and SnSAG 1 strains may be more prevalent than SnSAG 5 or SnSAG6 strains, all are implicated in canine protozoal encephalitis due to S. neurona. [ABSTRACT FROM AUTHOR]

Abstract: Thunderstorm simulation in the laboratory setting induces fearful and anxious behavior in beagles, most notably manifested by increased inactivity (“freezing”), which, in a companion study, was ameliorated by the anxiolytic diazepam (). Using this protocol, the present study assessed the efficacy of Harmonease, a chewable oral anxiolytic botanical product containing a proprietary blend of extracts of Magnolia officinalis and Phellodendron amurense. A balanced, placebo-controlled, blinded, single crossover design including 20 healthy adult beagles was used for this study. After a baseline thunderstorm test, subjects received Harmonease Chewable Tablets or placebo treatment daily and were reassessed on the treatment day 7. After a 7-day washout period, the treatments were crossed over and a design identical to that used in the first phase was used. The thunderstorm test was performed in an open-field arena (8 ft × 9 ft) and consisted of three 3-minute phases: an anticipatory phase in which no stimulus was provided; the thunderstorm phase in which a thunderstorm track was played over a speaker system; and a recovery phase in which no stimulus was presented. Inactivity duration was considered the primary variable for assessing efficacy which was measured by a trained observer. Difference in number of dogs improved versus worsened by treatment group was significant at P < 0.05. Specifically, 12 of 20 (60%) dogs improved from baseline when treated with Harmonease, whereas only 5 of 20 (25%) improved on placebo. Furthermore, 9 of 20 (45%) placebo dogs showed increased inactivity duration (worsened), whereas only 4 of 20 (20%) treatment dogs worsened. Increases in distance travelled consistent with reduced inactivity were also seen under Harmonease. Harmonease reduced fear-related inactivity or freezing in dogs in this thunderstorm simulation model. This supports past studies demonstrating that the combination of botanical extracts in Harmonease is effective in dogs for the management of stress-related behaviors. [Copyright &y& Elsevier]

The first pair of US Army animal-assisted therapy (AAT) dogs deployed to Iraq in December 2007 with the 85th Medical Detachment Combat and Operational Stress Control unit. As of this writing, 6 dogs have deployed to Iraq and Afghanistan, offering Soldiers a small reminder of home. Army occupational therapists led the way in this endeavor as primary handlers; the path has been rocky but ultimately rewarding. This article depicts how occupational therapists used AAT and animal-assisted activities to help Soldiers cope with the stressors of living in a deployed environment. Challenges and lessons-learned, including anecdotal examples, are discussed. [ABSTRACT FROM AUTHOR]

Combat and operational stress control (COSC) dogs represent a new category of military working dog. America's VetDogs, a nongovernmental, not-for-profit organization, trains and provides therapy dogs to work with the US Army's combat and operational stress control teams deployed to Afghanistan or Iraq. By taking the therapy/service dog concept to the next level, these dogs have become an important modality in the Army's initiative to safeguard Soldiers' behavioral health while deployed, allowing COSC unit members to break down stigmas that are still present when dealing with behavioral health issues. The training process begins by choosing a pool of dogs, exposing them to different sensory experiences over several months, and training the primary and secondary handlers who will be responsible for the dogs while deployed in theater. After their deployment ends, the dogs are retrained by America's VetDogs to further serve in military or Veterans Administration medical centers as physical, occupational, or behavioral therapy dogs. [ABSTRACT FROM AUTHOR]

Research has examined the physiological and psychosocial impact of animal-assisted activities (AAA) and animal-assisted therapy (AAT). The current review article summarizes the benefits of AAA and AAT for hospitalized patients with medical disorders, psychiatric patients, and residents of nursing homes and longterm care facilities. The literature regarding inclusion of animals in business and organizational settings is also reviewed. Although there is clear evidence of improved physical and psychological health from AAA and AAT in the civilian population, there is a dearth of published findings of the evaluation of such benefits for military personnel. [ABSTRACT FROM AUTHOR]

In this article, the author focuses on animals that are used in medical settings. It mentions the Americans with Disabilities Act (ADA) defines the use of service animals which says that dog are trained to perform tasks for the benefit of an individual with a disability. Further it mentions the animal-assisted therapy is an individualized healthcare treatment plan for individuals with physical, emotional, or cognitive dysfunction.

An introduction is presented in which the editor discusses various reports within the issue on topics including domesticated animals, various aspects of animal-assistance developments, and benefits of canine-assisted therapy in the civilian medical environment.

In this article, the author describes how the U.S. Army took the initiative to establish policies and standards across Army medicine for the use of Canines in Army Medicine. It mentions the U.S. Army formalize service and therapy animal use throughout all its medical activities. Further it discusses the governing the U.S. Department of Defense (DoD) regulation that establishes the processes to maintain human-animal bond programs, and animal-assisted therapy.

Summary The aim of this study was to validate two commercially available ELISA assays for total ghrelin measurement in dogs: one canine-specific and one originally designed for measuring human ghrelin. The two assays showed intra-assay coefficient of variations (CVs) lower than 10%, while the inter-assay CVs exceeded the 15% limit. Sample dilutions resulted in linear regression equations with correlation coefficients close to 1. In order to compare methods and verify ability of the ghrelin assays to differentiate between low and high levels, ghrelin concentrations were measured in plasma samples obtained before and at different times after glucose administration in five Beagle dogs. A statistically significant changes in ghrelin after glucose administration was recorded only with assay B. In conclusion, the human ELISA validated in this study showed a good intra-assay precision, accuracy, and when applied to the glucose injection study, was better in distinguishing high and low canine ghrelin levels than the canine ELISA assay. [ABSTRACT FROM AUTHOR]

Summary An autosomal recessive form of cerebellar abiotrophy occurs in Australian Kelpie dogs. Clinical signs range from mild ataxia with intention tremor to severe ataxia with seizures. A whole-genome mapping analysis was performed using Affymetrix Canine SNP array v2 on 11 affected and 19 control dogs, but there was no significant association with disease. A homozygosity analysis identified a three megabase region likely to contain the disease mutation. The region spans 29.8-33 Mb on chromosome 3, for which all affected dogs were homozygous for a common haplotype. Microsatellite markers were developed in the candidate region for linkage analysis that resulted in a logarithm of odds score suggestive of linkage. The candidate region contains 29 genes, none of which are known to cause ataxia. [ABSTRACT FROM AUTHOR]

Tail-chasing is widely celebrated as normal canine behaviour in cultural references. However, all previous scientific studies of tail-chasing or 'spinning' have comprised small clinical populations of dogs with neurological, compulsive or other pathological conditions; most were ultimately euthanased. Thus, there is great disparity between scientific and public information on tail-chasing. I gathered data on the first large (n = 400), non-clinical tail-chasing population, made possible through a vast, free, online video repository, YouTube™. The demographics of this online population are described and discussed. Approximately one third of tail-chasing dogs showed clinical signs, including habitual (daily or 'all the time') or perseverative (difficult to distract) performance of the behaviour. These signs were observed across diverse breeds. Clinical signs appeared virtually unrecognised by the video owners and commenting viewers; laughter was recorded in 55% of videos, encouragement in 43%, and the commonest viewer descriptors were that the behaviour was 'funny' (46%) or 'cute' (42%). Habitual tail-chasers had 6.5+/-2.3 times the odds of being described as 'Stupid' than other dogs, and perseverative dogs were 6.8+/-2.1 times more frequently described as 'Funny' than distractible ones were. Compared with breed- and age-matched control videos, tail-chasing videos were significantly more often indoors and with a computer/television screen switched on. These findings highlight that tail-chasing is sometimes pathological, but can remain untreated, or even be encouraged, because of an assumption that it is 'normal' dog behaviour. The enormous viewing figures that YouTube™ attracts (mean+/-s.e. = 863+/-197 viewings per tail-chasing video) suggest that this perception will be further reinforced, without effective intervention. [ABSTRACT FROM AUTHOR]

Abstract: We present the first report of a stray dog that recovered from canine compulsive disorder (CD)/obsessive compulsive disorder (OCD) leading to self-mutilation in Turkey. The dog was brought to a private veterinary clinic in Ankara with severe injuries on his rear paws and tail. During the routine clinical examination, it was noticed that he was displaying repetitive attacks to his hind end. A presumptive diagnosis of CD/OCD was made on the basis of the clinical examination and outdoor observation. After 6 months of treatment involving behavioral modification and medication, a large decrease in intensity and frequency of the CD was observed. [Copyright &y& Elsevier]

Abstract: The purpose of the study was to measure the effects of a dose of 0.2 mg/kg dextroamphetamine on body temperature, heart rate, motor activity, and associated behavior changes in Beagle dogs. Reliability of a collar-mounted accelerometer as an objective measure of motor activity was also investigated by comparing motor activity with that observed using video recordings. A total of 12 research colony Beagle dogs (13-20-months-old) served as their own control in this placebo-controlled crossover design, receiving both placebo and 0.2 mg/kg dextroamphetamine as treatment. Baseline and posttreatment values for body temperature, heart rate, and motor activity were obtained using a rectal temperature, heart rate monitor, and a collar-mounted accelerometer, respectively. Behavior sequences were filmed and analyzed. Repeated measures model indicated that dogs receiving a dose of 0.2 mg/kg dextroamphetamine had a significantly (P = 0.044) reduced heart rate as compared with placebo. There was no effect of treatment on the dogs’ body temperature, motor activity, or other behaviors such as “lip-licking,” “panting,” and “yawning.” There was a significant linear and positive relationship between the gross motor activity as measured by observational video and the accelerometer counts (P < 0.0001). Several behavioral textbooks used in clinical practice distinguish canine hyperactivity–hyperkinesis from overactivity by physiological and behavioral responses to amphetamines in a clinical setting. The authors of these textbooks suggest that true hyperactive–hyperkinetic dogs provided with oral amphetamines will paradoxically calm down, and have >15% reduction in heart rate. However, no data exist on the various effects of a low dose (0.2 mg/kg) of oral dextroamphetamine in dogs. The results of this study indicate that although as a group the medicated dogs showed a significantly lower heart rate than the placebo group, individual Beagle dogs showed variability in changes of heart rate. The use of the accelerometer in this study is a reliable tool for measuring motor activity in the dog. [Copyright &y& Elsevier]