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Although hundreds of keV in energy gain have already been demonstrated in dielectric laser accelerators (DLAs), the challenge of creating structures that can confine electrons for multiple millimeters remains. We focus here on dual gratings with single-sided drive, which have experimentally demonstrated energy modulation numerous times. Using a Finite-Difference Time-Domain simulation to find the fields within various DLA structures and correlating these results with particle tracking simulation, we look at the impact of teeth height and width, as well as gap and offset, on the performance of these structures. We find a tradeoff between electron throughput and acceleration; however, we also find that for any given grating geometry, there is a gap and offset that will allow some charge acceleration. For our 780 nm laser wavelength, this results in a 1200 nm optimal gap size for most gratings. [ABSTRACT FROM AUTHOR]

In dogs, symmetrical lupoid onychodystrophy (SLO) results in nail loss and an abnormal regrowth of the claws. In Bearded Collies, an autoimmune nature has been suggested because certain dog leukocyte antigen (DLA) class II haplotypes are associated with the condition. A genome-wide association study of the Bearded Collie revealed two regions of association that conferred risk for disease: one on canine chromosome (CFA) 12 that encompasses the DLA genes, and one on CFA17. Case-control association was employed on whole genome sequencing data to uncover putative causative variants in SLO within the CFA12 and CFA17 associated regions. Genotype imputation was then employed to refine variants of interest. Although no SLO-associated protein-coding variants were identified on CFA17, multiple variants, many with predicted damaging effects, were identified within potential candidate genes on CFA12. Furthermore, many potentially damaging alleles were fully correlated with the presence of DLA class II risk haplotypes for SLO, suggesting that the variants may reflect DLA class II haplotype association with disease or vice versa. Strong linkage disequilibrium in the region precluded the ability to isolate and assess the individual or combined effect of variants on disease development. Nonetheless, all were predictive of risk for SLO and, with judicious assessment, their application in selective breeding may prove useful to reduce the incidence of SLO in the breed.

The limited sensitivity of circulating tumor cell (CTC) detection in pancreatic adenocarcinoma (PDAC) stems from their extremely low concentration in the whole circulating blood, necessitating enhanced detection methodologies. This study sought to amplify assay-sensitivity by employing diagnostic leukapheresis (DLA) to screen large blood volumes. Sixty patients were subjected to DLA, with a median processed blood volume of ~ 2.8 L and approximately 5% of the resulting DLA-product analyzed using CellSearch (CS). Notably, DLA significantly increased CS-CTC detection to 44% in M0-patients and 74% in M1-patients, yielding a 60-fold increase in CS-CTC enumeration. DLA also provided sufficient CS-CTCs for genomic profiling, thereby delivering additional genomic information compared to tissue biopsy samples. DLA CS-CTCs exhibited a pronounced negative prognostic impact on overall survival (OS), evidenced by a reduction in OS from 28.6 to 8.5 months (univariate: p = 0.002; multivariable: p = 0.043). Additionally, a marked enhancement in sensitivity was achieved (by around 3-4-times) compared to peripheral blood (PB) samples, with positive predictive values for OS being preserved at around 90%. Prognostic relevance of CS-CTCs in PDAC was further validated in PB-samples from 228 PDAC patients, consolidating the established association between CTC-presence and reduced OS (8.5 vs. 19.0 months, p < 0.001). In conclusion, DLA-derived CS-CTCs may serve as a viable tool for identifying high-risk PDAC-patients and aiding the optimization of multimodal treatment strategies. Moreover, DLA enables comprehensive diagnostic profiling by providing ample CTC material, reinforcing its utility as a reliable liquid-biopsy approach. This high-volume liquid-biopsy strategy presents a potential pathway for enhancing clinical management in this malignancy. [ABSTRACT FROM AUTHOR]

Sequential pattern mining algorithms extract trendy sequence appearances inside ordered transactional datasets such as market basket datasets. There is a lack of research employing big data processing techniques to locate frequent sequences on large‐scale datasets. Furthermore, there is a need for optimized sequential pattern mining algorithms that run on ordered one‐dimensional sequences. We also observe a lack of sequential pattern search studies in the literature, where the focus is centered around multi‐dimensional data sequences. Existing approaches that deal with ordered one‐dimensional datasets suffer from scalability issues as the amount of data to be analyzed is enormous. This research investigates the big data processing techniques used to find frequent sequences in large‐scale datasets. It also proposes a scalable sequence pattern mining algorithm called Sequential Pattern Acquisition by Reducing Search Space (SPARSS) designed for distributed data processing systems that efficiently handle large datasets containing sequential one‐element data. It introduces a prototype implementation of SPARSS and provides information on the SPARSS's memory and time requirements, which were calculated as part of experimental studies on a real‐world dataset. The results confirm our expectations and demonstrate SPARSS's superior scalability and run‐time efficiency compared to other distributed algorithms. [ABSTRACT FROM AUTHOR]

The anti lymphoma potentials of Vernonia cinerea were evaluated. In a short-term in vitro study, ethyl acetate extract (VCEA) was found to be cytotoxic to DLA cells with an LC50 of 61.24 μg mL−1. GC-MS analysis of VCEA revealed presence of several volatile molecules including nerolidol. Molecular docking study using nerolidol with Ferroportin-1 predicted strong binding affinity, indicating ferroptosis induction which was later confirmed by in vitro assays. VCEA also demonstrated strong reduction in the proliferation of YAC-1, another lymphoma cell line with G2/M arrest leading to apoptosis. The tumor reduction potentials of VCEA was further confirmed using the DLA-induced solid tumor model in mice. [ABSTRACT FROM AUTHOR]

Low-power pulsed laser-MAG hybrid welding was carried out by different heat source arrangement methods (lead mode, distance between laser spot and welding wire tip (DLA)). The coupling effect and welding characteristics of hybrid heat sources under different modes were studied by using high-speed image (HSI) and real-time electrical signals. HSI observation and electronic signals showed that the lead mode and DLA significantly affected the coupling state of the heat source and welding characteristics (weld morphology, process stability, and droplet transfer). The influence of lead mode on weld forming was more significant than that of DLA. In laser-lead mode, when DLA = 1 ~ 2 mm, the laser and arc generated a perfect coupling effect, resulting in a good weld formation, less spatter, and greater penetration. In arc-lead mode, the weld formation was poor and the undercut defect was common, while the penetration was greater than that in laser-lead mode. In terms of welding process stability, the arc-lead mode was better than the laser-lead mode. When DLA = 1 ~ 2 mm, stable droplet transfer could be realized under both modes. [ABSTRACT FROM AUTHOR]

BackgroundPrimary hypoadrenocorticism (Addison's disease, AD) and symmetrical lupoid onychodystrophy (SLO) are two clinical conditions with an autoimmune etiology that occur in multiple dog breeds. In man, autoimmunity is associated with polymorphisms in immune-related genes that result in a reduced threshold for, or defective regulation of, T cell activation. The major histocompatibility complex (MHC) class II genes encode molecules that participate in these functions, and polymorphisms within these genes have been associated with autoimmune conditions in dogs and humans. Bearded collies have a relatively high prevalence of autoimmune diseases, particularly AD and SLO. Our study assessed the relationship between particular MHC (dog leukocyte antigen, DLA) class II haplotypes and the two autoimmune diseases most common in this breed. Moreover, five unrelated breeds at increased risk for AD were studied for comparative purposes and analyzed in the context of extant literature.ResultsA single DLA class II three-locus haplotype, determined by sequence-based typing, was associated with increased risk for AD (DLA-DRB1*009:01/DQA1*001:01/DQB1*008:02) in bearded collies. Comparative analysis with the five additional breeds showed limited allele sharing, with DQA1*001:01 and DQB1*002:01 being the only alleles observed in all breeds. A distinct three-locus risk haplotype (DLA-DRB1*001:01/DQA1*001:01/DQB1*002:01) was associated with AD in the West Highland white terrier and Leonberger. Two different risk haplotypes were associated with increased risk for SLO in the bearded collie (DLA-DRB1*018:01/DQA1*001:01/DQB1*002:01 and DLA-DRB1*018:01/DQA1*001:01/ DQB1*008:02).ConclusionTwo-locus DQ haplotypes composed of DLA-DQA1*001:01 in association with DLA-DQB1*002:01 or DLA-DQB1*008:02 make up the four risk haplotypes identified in the present study and are also found in other risk haplotypes previously associated with diabetes mellitus and hypothyroidism across different dog breeds. Our findings build upon previously published data to suggest that this two-locus (DQ) model serves as a good indicator for susceptibility to multiple organ-specific autoimmune diseases in the canine population. However, it is also clear that additional loci are necessary for actual disease expression. Investigation of affected and unaffected dogs carrying these predisposing DQ haplotype signatures may allow for the identification of those additional genetic components that determine autoimmune disease expression and organ specificity.

ObjectiveSudden acquired retinal degeneration syndrome (SARDS) is one of the leading causes of acute blindness in dogs, with an unknown etiology and no effective treatment. Certain breeds such as Dachshunds are overrepresented among SARDS patients, and therefore, the syndrome is suspected to have a genetic component. The objective of this study was to determine if a genetic locus associated with SARDS in Dachshunds could be identified using a genome-wide association study (GWAS).ProceduresGenome-wide association mapping was performed in 15 SARDS-affected and 16 unaffected Dachshunds. Genotyping of three classical DLA class II genes (DLA-DRB1, DLA-DQA1, and DLA-DQB1) was performed in 34 SARDS-affected and 66 unaffected Dachshunds to evaluate for an association in this region.ResultsAlthough no single nucleotide polymorphisms (SNPs) were of genome-wide statistical significance (PBonferroni

Symmetrical lupoid onychodystrophy (SLO) is characterized by inflammation of the nail bed and nail sloughing that causes affected dogs considerable pain. Disease etiology remains unclear, although an autoimmune component is suspected. A genome-wide association study on Bearded Collies revealed regions of association on canine chromosomes (CFA) 12 and 17. The large region of association on CFA12 likely consists of two smaller linked regions, both of which are also linked to the dog leukocyte antigen (DLA) class II genes. Dogs homozygous for the alternate allele at the top CFA12 SNP also carried two DLA class II risk haplotypes for SLO, and this locus explained most of the increased risk for disease seen throughout the CFA12 region of association. A stronger peak was seen on CFA17 when analysis was done solely on dogs that carried DLA class II risk haplotypes for SLO. The majority of SLO dogs carried a homozygous alternate genotype on CFA12 and at least one CFA17 risk haplotype. Our findings offer progress toward uncovering the genetic basis of SLO. While the contribution of the CFA17 region remains unclear, both CFA12 and CFA17 regions are significantly associated with SLO disease expression in the Bearded Collie and contain potential candidate genes for this disease.

Although hundreds of keV in energy gain have already been demonstrated in dielectric laser accelerators (DLAs), the challenge of creating structures that can confine electrons for multiple millimeters remains. We focus here on dual gratings with single-sided drive, which have experimentally demonstrated energy modulation numerous times. Using a Finite-Difference Time-Domain simulation to find the fields within various DLA structures and correlating these results with particle tracking simulation, we look at the impact of teeth height and width, as well as gap and offset, on the performance of these structures. We find a tradeoff between electron throughput and acceleration; however, we also find that for any given grating geometry, there is a gap and offset that will allow some charge acceleration. For our 780 nm laser wavelength, this results in a 1200 nm optimal gap size for most gratings.

Abstract Background In Europe, golden jackals (Canis aureus) have been expanding their range out of the southern and southeastern Balkans towards central Europe continually since the 1960s. Here, we investigated the level of functional diversity at the MHC class II DLA-DQA1 exon 2 in golden jackal populations from Bulgaria, Serbia, and Hungary. Specifically, we tested for positive selection on and geographic variation at that locus due to adaptation to supposedly regionally varying pathogenic landscapes. To test for potential fitness effects of different protein variants on individual body condition, we used linear modeling of individual body mass indexes (bmi) and accounted for possible age, sex, geographical, and climatic effects. The latter approach was performed, however, only on Serbian individuals with appropriate data. Results Only three different DLA-DQA1 alleles were detected, all coding for different amino-acid sequences. The neutrality tests revealed no significant but positive values; there was no signal of spatial structuring and no deviation from the Hardy–Weinberg equilibrium across the studied range of expansion. However, we found a signal of trans-species polymorphism and significant test results for positive selection on three codons. Our information-theory based linear modeling results indicated an effect of ambient temperature on the occurrence of individual DLA-DQA1 genotypes in individuals from across the studied expansion range, independent from geographical position. Our linear modeling results of individual bmi values indicated that yearlings homozygous for DLA-DQA1*03001 reached values typical for adults contrary to yearlings carrying other genotypes (protein combinations). This suggested better growth rates and thus a possible fitness advantage of yearlings homozygous for DLA-DQA1*03001. Conclusions Our results indicate a demographic (stochastic) signal of reduced DLA-DQA1 exon 2 variation, in line with the documented historical demographic bottleneck. At the same time, however, allelic variation was also affected by positive selection and adaptation to varying ambient temperature, supposedly reflecting geographic variation in the pathogenic landscape. Moreover, an allele effect on body mass index values of yearlings suggested differential fitness associated with growth rates. Overall, a combination of a stochastic effect and positive selection has shaped and is still shaping the variation at the studied MHC locus.

The organic sulfur compounds dimethylsulfoniopropionate (DMSP) and dimethyl sulfoxide (DMSO) play major roles in the marine microbial food web and have substantial climatic importance as sources and sinks of dimethyl sulfide (DMS). Seasonal shifts in the abundance and diversity of the phytoplankton and bacteria that cycle DMSP are likely to impact marine DMS (O) (P) concentrations, but the dynamic nature of these microbial interactions is still poorly resolved. Here, we examined the relationships between microbial community dynamics with DMS (O) (P) concentrations during a 2-year oceanographic time series conducted on the east Australian coast. Heterogenous temporal patterns were apparent in chlorophyll a (chl a) and DMSP concentrations, but the relationship between these parameters varied over time, suggesting the phytoplankton and bacterial community composition were affecting the net DMSP concentrations through differential DMSP production and degradation. Significant increases in DMSP were regularly measured in spring blooms dominated by predicted high DMSP-producing lineages of phytoplankton (Heterocapsa , Prorocentrum , Alexandrium , and Micromonas), while spring blooms that were dominated by predicted low DMSP-producing phytoplankton (Thalassiosira) demonstrated negligible increases in DMSP concentrations. During elevated DMSP concentrations, a significant increase in the relative abundance of the key copiotrophic bacterial lineage Rhodobacterales was accompanied by a three-fold increase in the gene, encoding the first step of DMSP demethylation (dmdA). Significant temporal shifts in DMS concentrations were measured and were significantly correlated with both fractions (0.2–2 μm and >2 μm) of microbial DMSP lyase activity. Seasonal increases of the bacterial DMSP biosynthesis gene (dsyB) and the bacterial DMS oxidation gene (tmm) occurred during the spring-summer and coincided with peaks in DMSP and DMSO concentration, respectively. These findings, along with significant positive relationships between dsyB gene abundance and DMSP, and tmm gene abundance with DMSO, reinforce the significant role planktonic bacteria play in producing DMSP and DMSO in ocean surface waters. Our results highlight the highly dynamic nature and myriad of microbial interactions that govern sulfur cycling in coastal shelf waters and further underpin the importance of microbial ecology in mediating important marine biogeochemical processes. [ABSTRACT FROM AUTHOR]

The organic sulfur compounds dimethylsulfoniopropionate (DMSP) and dimethyl sulfoxide (DMSO) play major roles in the marine microbial food web and have substantial climatic importance as sources and sinks of dimethyl sulfide (DMS). Seasonal shifts in the abundance and diversity of the phytoplankton and bacteria that cycle DMSP are likely to impact marine DMS (O) (P) concentrations, but the dynamic nature of these microbial interactions is still poorly resolved. Here, we examined the relationships between microbial community dynamics with DMS (O) (P) concentrations during a 2-year oceanographic time series conducted on the east Australian coast. Heterogenous temporal patterns were apparent in chlorophyll a (chl a) and DMSP concentrations, but the relationship between these parameters varied over time, suggesting the phytoplankton and bacterial community composition were affecting the net DMSP concentrations through differential DMSP production and degradation. Significant increases in DMSP were regularly measured in spring blooms dominated by predicted high DMSP-producing lineages of phytoplankton (Heterocapsa, Prorocentrum, Alexandrium, and Micromonas), while spring blooms that were dominated by predicted low DMSP-producing phytoplankton (Thalassiosira) demonstrated negligible increases in DMSP concentrations. During elevated DMSP concentrations, a significant increase in the relative abundance of the key copiotrophic bacterial lineage Rhodobacterales was accompanied by a three-fold increase in the gene, encoding the first step of DMSP demethylation (dmdA). Significant temporal shifts in DMS concentrations were measured and were significantly correlated with both fractions (0.2–2 μm and >2 μm) of microbial DMSP lyase activity. Seasonal increases of the bacterial DMSP biosynthesis gene (dsyB) and the bacterial DMS oxidation gene (tmm) occurred during the spring-summer and coincided with peaks in DMSP and DMSO concentration, respectively. These findings, along with significant positive relationships between dsyB gene abundance and DMSP, and tmm gene abundance with DMSO, reinforce the significant role planktonic bacteria play in producing DMSP and DMSO in ocean surface waters. Our results highlight the highly dynamic nature and myriad of microbial interactions that govern sulfur cycling in coastal shelf waters and further underpin the importance of microbial ecology in mediating important marine biogeochemical processes.

[Display omitted] Understanding deagglomeration, agglomerate formation and structure for very small nanoparticles (NPs), due to their more facile agglomeration, is critical for processing or tailoring agglomerates for nanostructured materials. We propose that by controlling and fine-tuning the interplay of agglomeration (colloidal interaction) and deagglomeration (hydrodynamic forces), the design of agglomerate size, microstructure and morphology is possible even for very small NPs. Here, we investigate very small SnO 2 NPs (10 nm) generated in the gas phase as model system. Small-angle X-ray scattering (SAXS) and dynamic light scattering (DLS) are used to study dispersions in aqueous media across the entire pH range (2–12) at various NaCl concentrations treated with ultrasound. Parallel to size and size distribution, agglomerate morphology and microstructure are analyzed by means of the mass fractal dimension, d m and modeled with ab initio shape simulations. The critical coagulation concentration (CCC) is determined for the alkaline region where the SnO 2 NPs are highly charged. Quantitative analysis of SAXS and DLS data reveals that size and size distribution of the agglomerates depend similarly on the electrostatic interaction influenced by pH and salinity as observed by the zeta potential. In contrast d m is mainly influenced by the salt concentration. Ab initio shape simulations support these experimental findings. [ABSTRACT FROM AUTHOR]

Aim: The aim of the study was to assess the effect of changes in population size and migration on variation in functional immunity genes in the previously bottlenecked population of the grey wolf, Canis lupus. Location: Eastern/Central Europe: Poland, Czechia, Slovakia. Methods: We genotyped 7 immunity genes: three MHC‐DLA genes (dog leukocyte antigen) and four Toll‐like receptor (TLR) genes among 130 wolves originating from three populations: two lowland (Baltic and Central European), and highland Carpathian. We contrasted the population structure in immunity genes with a neutral structure based on 13 microsatellites, and we analysed signatures of selection in the immunity loci. Results: We found high overall genetic variance in immunity genes and no evidence for decreased diversity in the recently established populations. The population structure in immunity loci was weak, with pairwise FST lower than for neutral markers. Although the results of neutrality tests were not significant, we identified codons under selection, both positive and negative. Main Conclusions: We demonstrated that despite recent population expansion which is expected to result in decreased genetic diversity, the diversity of immunity genes in the newly established wolf population is similar to those in the source population. This suggests that migrations do not cause allele loss in grey wolf. Signatures of selection on codon level, but not in tests using allele frequencies, suggest the contrasting effects of demography and selection. [ABSTRACT FROM AUTHOR]

MHC genes are highly polymorphic as antigen presenting molecules for adaptive immune response in vertebrates. In this study, we evaluated the diversity of dog leukocyte antigen (DLA) class II genes among two Korean breeds, the Sapsaree and the Jindo, and three Chinese native breeds, Tibetan Mastiff, Pug, and Pekingese, and determined their genetic relationships. Sequence-based typing of DLA-DRB1 and DLA-DQB1 was performed in 138 dogs. We identified 27 alleles for DRB1 and 24 for DQB1, including five new alleles. DRB1*015:01 was shared among all five breeds and was a major allele for the Pug, Tibetan Mastiff, and Sapsaree. The observed heterozygosity for the five breeds ranged from 0.67 to 1.00 for DRB1 and from 0.80 to 1.00 for DQB1. A total of 40 DRB1-DQB1 haplotypes, including nine new haplotypes, were identified. Interestingly, most haplotypes (33/40, 82%) were specific to a single breed, and only seven were present in multiple breeds. Haplotype sharing analysis together with previously available data from 109 breeds revealed that compared to within region distances, Asian breeds were more distant than breeds from other regions. As a first report on the analysis of DLA genes of dog breeds in Korean peninsula, our results show that these breeds carry unique DLA class II haplotype lineages. Our results indicate that the diversity and breed specificity of DLA class II genes are much higher among East Asian breeds than among breeds from other regions. [ABSTRACT FROM AUTHOR]

• The fractal growth of chromium dendrites in electrodeposition was studied. • The DLA simulation results are consistent with the actual experimental results. • Dendrite morphology is closely related to particle motion. • The dendritic clusters are primarily composed of three-dimensional grains. This investigated the fractal growth behavior of dendrites during the electrodeposition of chromium metal within two-dimensional confined interface. The effect of influencing factors (voltage, electrolyte concentration, catalyst concentration, and deposition time) on the morphology and fractal dimension of the deposited dendrites was investigated using zero-dimensional point electrodes and one-dimensional wire electrodes. The results indicate that as the voltage and electrolyte concentration increase, the fractal dimension of the point electrode decreases, while that of the wire electrode increases. Additionally, increasing the concentration of the catalyst and the deposition time led to an increase in the fractal dimension for both the point electrode and the wire electrode. This is due to the morphology of the dendritic clusters growing more open or denser depending on the aggregation mode of particles under different experimental conditions. This further leads to changes in fractal dimension. A diffusion-limited aggregation (DLA) model was developed to simulate the motion of particles during deposition. The model demonstrates that the changes in fractal dimension and the morphology of the aggregates formed by particles moving randomly are consistent with the experimental results. Finally, the distribution of grains on the sediment surface revealing that fractal dendrite clusters in a two-dimensional sediment interface consist of three-dimensional grains. [ABSTRACT FROM AUTHOR]

Background: In Europe, golden jackals (Canis aureus) have been expanding their range out of the southern and southeastern Balkans towards central Europe continually since the 1960s. Here, we investigated the level of functional diversity at the MHC class II DLA-DQA1 exon 2 in golden jackal populations from Bulgaria, Serbia, and Hungary. Specifically, we tested for positive selection on and geographic variation at that locus due to adaptation to supposedly regionally varying pathogenic landscapes. To test for potential fitness effects of different protein variants on individual body condition, we used linear modeling of individual body mass indexes (bmi) and accounted for possible age, sex, geographical, and climatic effects. The latter approach was performed, however, only on Serbian individuals with appropriate data. Results: Only three different DLA-DQA1 alleles were detected, all coding for different amino-acid sequences. The neutrality tests revealed no significant but positive values; there was no signal of spatial structuring and no deviation from the Hardy-Weinberg equilibrium across the studied range of expansion. However, we found a signal of trans-species polymorphism and significant test results for positive selection on three codons. Our information-theory based linear modeling results indicated an effect of ambient temperature on the occurrence of individual DLA-DQA1 genotypes in individuals from across the studied expansion range, independent from geographical position. Our linear modeling results of individual bmi values indicated that yearlings homozygous for DLA-DQA1*03001 reached values typical for adults contrary to yearlings carrying other genotypes (protein combinations). This suggested better growth rates and thus a possible fitness advantage of yearlings homozygous for DLA-DQA1*03001. Conclusions: Our results indicate a demographic (stochastic) signal of reduced DLA-DQA1 exon 2 variation, in line with the documented historical demographic bottleneck. At the same time, however, allelic variation was also affected by positive selection and adaptation to varying ambient temperature, supposedly reflecting geographic variation in the pathogenic landscape. Moreover, an allele effect on body mass index values of yearlings suggested differential fitness associated with growth rates. Overall, a combination of a stochastic effect and positive selection has shaped and is still shaping the variation at the studied MHC locus. [ABSTRACT FROM AUTHOR]

A model of particle-cluster aggregation on the rewired small-world network is studied. In this model, particles can adjoin to the end of the rewired long-range edges. The rewired long-range edge is controlled by two parameters, the reconnecting probability and distance controlling parameter. The simulation results indicate that, with the increasing of the length and the number of rewired long-range edges, the patterns become diffuse and the fractal dimensions of the aggregates decrease. The range of the value of dimension and the feature of patterns obtained in the model are consistent with the amyloid deposits. [ABSTRACT FROM AUTHOR]

This study focused on identification of antiproliferative mechanism of Murva by in silico methods and compared the in vivo anticancer activity of methanol extracts of Marsdenia tenacissima (MEMT) and Sansevieria roxburghiana (MESR), are known to be Murva. PASS online study was carried out with Dresgenin from M.tenacissima and Lupeol from S.roxburghiana to know the possible biological activities and were taken as ligands for the target BCL-2 protein. Tryphan blue dye exclusion assay was done with different concentration of MEMT and MESR. In in vivo, 90 mice were organized in to 9 groups with ten mice in each. All animals except group I, intraperitoneal inoculated with DLA cell lines. Group I-normal control received distilled water, Group II cancer control received distilled water, Group III -received 20mg/kg of standard 5-Fluorouracil, i.p, Group IV - 100mg/kg of Venetoclax, p.o, Group V &VI treated with 100 and 200mg/kg of MEMT, Group VII &VIII treated with 50 and 100mg/kg of MESR and IX received combination of MEMT & MESR for 14 days. On 15th day haematological and tissue antioxidant level were measured. Insilico study results revealed that ligand has antineoplastic and Caspase 3 stimulant activity, binding scores of -9.1 and -9.2 (Kcal/mol) with BCL-2. In vitro cytotoxicity result showed MEMT (56%), MESR (68%) and the combination (92%) of cell death. In in vivo study, most significant (p<0.001) effect with combination on altered haematological and antioxidant level. The pharmacological effect was due the binding of Dresgenin and Lupeol binds with BCL-2 protein. [ABSTRACT FROM AUTHOR]

We approach the problem of implementing mixed-datatype support within the general matrix multiplication (gemm) operation of the BLAS-like Library Instantiation Software framework, whereby each matrix operand A, B, and C may be stored as single- or double-precision real or complex values. Another factor of complexity, whereby the matrix product and accumulation are allowed to take place in a precision different from the storage precisions of either A or B, is also discussed. We first break the problem into orthogonal dimensions, considering the mixing of domains separately from mixing precisions. Support for all combinations of matrix operands stored in either the real or complex domain is mapped out by enumerating the cases and describing an implementation approach for each. Supporting all combinations of storage and computation precisions is handled by typecasting the matrices at key stages of the computation—during packing and/or accumulation, as needed. Several optional optimizations are also documented. Performance results gathered on a 56-core Marvell ThunderX2 and a 52-core Intel Xeon Platinum demonstrate that high performance is mostly preserved, with modest slowdowns incurred from unavoidable typecast instructions. The mixed-datatype implementation confirms that combinatorial intractability is avoided, with the framework relying on only two assembly microkernels to implement 128 datatype combinations. [ABSTRACT FROM AUTHOR]

Abstract Background Primary hypoadrenocorticism (Addison’s disease, AD) and symmetrical lupoid onychodystrophy (SLO) are two clinical conditions with an autoimmune etiology that occur in multiple dog breeds. In man, autoimmunity is associated with polymorphisms in immune-related genes that result in a reduced threshold for, or defective regulation of, T cell activation. The major histocompatibility complex (MHC) class II genes encode molecules that participate in these functions, and polymorphisms within these genes have been associated with autoimmune conditions in dogs and humans. Bearded collies have a relatively high prevalence of autoimmune diseases, particularly AD and SLO. Our study assessed the relationship between particular MHC (dog leukocyte antigen, DLA) class II haplotypes and the two autoimmune diseases most common in this breed. Moreover, five unrelated breeds at increased risk for AD were studied for comparative purposes and analyzed in the context of extant literature. Results A single DLA class II three-locus haplotype, determined by sequence-based typing, was associated with increased risk for AD (DLA-DRB1*009:01/DQA1*001:01/DQB1*008:02) in bearded collies. Comparative analysis with the five additional breeds showed limited allele sharing, with DQA1*001:01 and DQB1*002:01 being the only alleles observed in all breeds. A distinct three-locus risk haplotype (DLA-DRB1*001:01/DQA1*001:01/DQB1*002:01) was associated with AD in the West Highland white terrier and Leonberger. Two different risk haplotypes were associated with increased risk for SLO in the bearded collie (DLA-DRB1*018:01/DQA1*001:01/DQB1*002:01 and DLA-DRB1*018:01/DQA1*001:01/ DQB1*008:02). Conclusion Two-locus DQ haplotypes composed of DLA-DQA1*001:01 in association with DLA-DQB1*002:01 or DLA-DQB1*008:02 make up the four risk haplotypes identified in the present study and are also found in other risk haplotypes previously associated with diabetes mellitus and hypothyroidism across different dog breeds. Our findings build upon previously published data to suggest that this two-locus (DQ) model serves as a good indicator for susceptibility to multiple organ-specific autoimmune diseases in the canine population. However, it is also clear that additional loci are necessary for actual disease expression. Investigation of affected and unaffected dogs carrying these predisposing DQ haplotype signatures may allow for the identification of those additional genetic components that determine autoimmune disease expression and organ specificity.

The immune system is one of the most important biological systems of our body. Immunomodulation is either increase or decrease in the immune response. Objective: To evaluate the immunomodulatory activity of the leaves of Thespesia populnea (Family Malvaceae) in cancer cell line induced Balb/c mice. Methods: Various extracts of the leaves of Thespesia populnea (TpFf) were evaluated for the flavonoid content and the higher content of flavonoid was found to be in ethyl acetate extract and it was used to evaluate the potential immunomodulatory activity. The ethyl acetate flavonoid extract was evaluated for immunomodulatory activity in in vivo studies, using Balb/c mice as the animal model. The extract was tested for hematological parameters, immunological parameters, weight and Cellularity of lymphoid organs and Histopathological observation of spleen and thymus, using sheep red blood cells (SRBC) as the antigen. PBS served as a control in all the tests. Findings: The ethyl acetate extract exhibited a significant decrease in the WBC and increase in the RBC and Hb when compared to Ehrlich's lymphoma ascites (ELA) and Dalton's lymphoma ascites (DLA) induced groups. It also resulted in a significant increase in the IL-2, IFN-γ, weight of spleen and thymus and Cellularity of lymphoid organs and malignant changes observed in the DLA and EAC tumor induced spleen and thymus sections. Conclusion: The ethyl acetate extract was found to stimulate the immune responses in Balb/c mice induced ELA and DLA. [ABSTRACT FROM AUTHOR]

Almost all efforts to optimize high-performance matrix-matrix multiplication have been focused on the case where matrices contain real elements. The community's collective assumption appears to have been that the techniques and methods developed for the real domain carry over directly to the complex domain. As a result, implementors have mostly overlooked a class of methods that compute complex matrix multiplication using only real matrix products. This is the second in a series of articles that investigate these so-called induced methods. In the previous article, we found that algorithms based on the more generally applicable of the two methods--the 4M method--lead to implementations that, for various reasons, often underperform their real domain counterparts. To overcome these limitations, we derive a superior 1M method for expressing complex matrix multiplication, one which addresses virtually all of the shortcomings inherent in 4M. Implementations are developed within the BLIS framework, and testing on microarchitectures by three vendors confirms that the 1M method yields performance that is generally competitive with solutions based on conventionally implemented complex kernels, sometimes even outperforming vendor libraries. [ABSTRACT FROM AUTHOR]

This is the first study to look at the characteristics of funds accepting the $2.7 trillion taxpayer guarantee of money market mutual funds during the 2008 financial crisis. Fund shares that benefited from Federal Reserve's asset-backed commercial paper program were significantly more likely to get bailed out by taxpayers and sponsors. The paper tests if funds adhering to the SEC's 2010 liquidity reforms prior to their enactment were less likely to be bailed out in 2008. Finally, it examines if funds subject to the 2014 floating NAV regulations were more likely to be bailed out in 2008. [ABSTRACT FROM AUTHOR]

This paper introduces a modified DLA model, based on a fractional diffusion mechanism, as a novel approach to modeling fractal growth. The specific memory performance of fractional operators can be reflected macroscopically in aggregated patterns eventually. The influence of the model's order on the structure and behavior of its pattern is further quantitatively described by anisotropy index and fractal dimension. Some simulations are provided to illustrate the correctness and effectiveness of the main results. [ABSTRACT FROM AUTHOR]

Addison's disease (AD) is a life-threatening endocrine disorder that occurs spontaneously in both humans and dogs. Associations between MHC class II genes and AD have been shown in several human studies. Our goal was to identify MHC class II associations with AD in a large population of Standard Poodles, a breed highly predisposed to AD. We sequenced exon 2 of the class II genes DLA-DRB1, DLA-DQA1, and DLA-DQB1 in 110 affected and 101 unaffected Standard Poodles and tested for association with AD. After correcting for population structure, two haplotypes were found to confer risk of developing AD in a sex-specific manner: DLA-DRB1*015:01-DQA1*006:01-DQB1*023:01 in males (x2p = 0.03, OR 2.1) and DLA-DRB1*009:01-DQA1*001:01-DQB1*008:01:1 in females (x2p = 0.02, OR 8.43). Sex-specific associations have been previously described in human populations, but this is the first report of this kind in dogs. Consistent with findings in other studies, we found the DLA-DQA1*006:01 allele (x2p = 0.04) to be associated with AD in males independent of haplotype. In females, the haplotype DLA-DRB1*009:01-DQA1*001:01-DQB1*008:01:1 confers a very high risk for developing AD, although its frequency was rare (9 of 124 females) in our study population. Further studies are warranted to validate the findings of this exploratory dataset and to assess the usefulness of this haplotype as a risk marker for AD in female Standard Poodles. Our results highlight the importance of evaluating MHC class II disease associations in large populations, and accounting for both biological sex and population structure. [ABSTRACT FROM AUTHOR]

• Low power pulsed laser-induced MAG hybrid welding can be applied to welding at a certain workpiece inclination angle, with excellent welding stability, proving its potential for application in all position welding. • The mechanism of D LA and workpiece inclination angle on droplet transfer in low power pulsed laser-induced MAG hybrid welding was revealed. • This work provides a theoretical basis for the popularization and application of laser-induced MAG all-position hybrid welding technology. In this work, the effects of workpiece inclination angles (θ), distance between laser spot and wire tip (D LA) on arc morphology, droplet transfer characteristics and stability were investigated by high-speed images (HSI), mathematical statistics and real-time electric signals. The coupling effect of heat source was evaluated by taking penetration as the main reference index. At the same time, the stability of the droplet transfer was evaluated based on the droplet transfer characteristics and the variation coefficient of current and voltage. The results showed that D LA had significant effect on droplet transfer in laser- induced MAG hybrid welding with varied workpiece angles. At D LA was 1 mm, the laser plasma compressed the arc, leading to the increase of the electromagnetic resistance of droplet transfer, which would prolong the formation and shedding time of droplet, making the droplet transfer mode change from "multi drop per pulse" to "one drop per pulse", and improving the stability of droplet transfer. The D LA affected the droplet transfer path and space instantaneous flight velocity. When D LA was −1 and 1 mm, the displacement of the droplet transfer reaches the minimum and maximum, respectively. When D LA was 1 mm, the droplet transfer was the most stable and maximum velocity could be obtained with varied workpiece inclination angles. This work provides a theoretical basis for the popularization and application of laser-induced MAG all-position hybrid welding technology. [ABSTRACT FROM AUTHOR]

Se emplea el plasma de la descarga luminiscente anormal DLA en la remoción del lubricante de compactos de hierro pulvimetalúrgico en atmósfera de hidrógeno, con el fin de establecer las condiciones óptimas del ciclo térmico en la configuración catódica para mantener las propiedades ofrecidas del hierro. Para la preparación de las mezclas se utiliza un mezclador rotatorio. El material se conforma con presión uniaxial en matriz cilíndrica, calculando su densidad y porosidad. Con TGA y DSC se estudian la descomposición térmica y la eliminación del lubricante; luego, se realizan los ciclos de remoción en la DLA. Con TGA se monitorea el estado de remoción del lubricante de cada ciclo en la DLA. Se caracterizan los compactos en verde y los del proceso de remoción con microscopia óptica y SEM. Se halló una adecuada remoción en la configuración catódica a 400 °C durante 10 minutos, removiéndose la totalidad de lubricante. El ciclo propuesto conviene en aplicaciones industriales por las altas tasas de calentamiento alcanzadas con reducción del consumo de energía, así como de la necesaria en mantener la descarga, referidas a los hornos resistivos utilizados.

The main aim of the article is to establish the status of expression dla odmiany in Polish; attention focuses both on the conceptual level of language analyzed in the syntactic-semantic perspective and also on the metatextual level described in functional terms. The expressions like dla zdrowia, dla zabawy and also dla przykładu, dla przypomnienia serve as a background for this analysis. The obtained results demonstrate that the uses of dla odmiany represent: 1) syntactical construction, which realizes two patterns: a) zrobić coś [dla _ {np. odmiany (od czegoś)}], b) [(zrobić coś1)] dla _ {np. odmiany} (zrobić coś2) and 2) metatextual language unit dla odmiany. [ABSTRACT FROM AUTHOR]

Diagnostic leukapheresis (DLA) is based on continuous centrifugation that collects mononuclear cells from peripheral blood with a density of 1.055–1.08 g/ml. As epithelial cells have a similar density, DLA cocollects circulating tumor cell (CTCs) along with the targeted mononuclear cells. Here, we report on our single center experience applying DLA in 40 nonmetastatic and metastatic breast cancer patients and its impact on CTC detection. We found that the use of just 5% of the DLA product (corresponding to a median peripheral blood volume of around 60 ml) in the CellSearch® assay already leads to a significant increase in CTC detection frequency and yield. The implementation of the method was unproblematic, and we did not observe any adverse events in our patient cohort. Extrapolating the CTC counts in the DLA samples to the whole DLA product indicated that enormous CTC numbers could be harvested by this approach (around 205x more CTCs than in the 7.5 ml blood sample in M1 patients). In conclusion, DLA is a clinically safe method to collect CTCs from liters of blood enabling a real liquid biopsy. Yet, further technical developments are required to process whole DLA products and exploit the full potential of this approach. As it is foreseeable that DLA will be used by several groups, and hopefully ultimately brought to the patients in a routine setting, we discuss recommendations on the minimum of required information for reporting on DLAs to allow comparison across different approaches. © 2018 International Society for Advancement of Cytometry [ABSTRACT FROM AUTHOR]

Abstract We present a methodology for designing and measuring pulse front tilt in an ultrafast laser for use in dielectric laser acceleration. Previous research into dielectric laser accelerating modules has focused on measuring high accelerating gradients in novel structures, but has done so only for short electron–laser coupling lengths. Here we demonstrate an optical design to extend the laser–electroninteraction to 1 mm. [ABSTRACT FROM AUTHOR]

A series of laser-tungsten inert gas hybrid welding with filler wire experiments were performed on the TA15 titanium alloys to investigate the effect of welding parameters, such as the distance between the axis of the laser beam and the tip of the tungsten electrode (Dla), the defocused distance of the laser beam, and its CO2 concentration in the shielding gas, on the weld appearance and dimension. The mechanism of the weld formation was then analyzed. The results demonstrated that by adjusting space position between the heat sources and protecting the shielding gas content, the undercut defect could be suppressed effectively in the hybrid welding process. With the increase in the Dla, defocused distance, and CO2 concentration, the width of the top weld presented an increasing trend, while the width and reinforcement of the backside showed a decreasing trend. The ridgelike appearance may be effectively suppressed if the Dla, defocused distance of the laser beam, and CO2 concentration in the argon shielding gas were more than 2 mm, 4 mm, and 2%, respectively, because the Dla, defocused distance, and CO2-determined energy concentration, surface tension, and Marangoni convection determine the molten metal flow direction. [ABSTRACT FROM AUTHOR]

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