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Aiming at the vibration reduction problems of pipeline systems under high temperature, a high temperature mechanics model of the metal rubber coated damping structure of the pipe-lines was established by equivalent linearization method. The model was used for analyzing the stiffness and response amplitude of metal rubber treated with different temperatures. The thermal-vibration joint test system of the pipelines was set up firstly. Then the damping performance of the coated damping structure of the pipelines was verified with different ambient temperatures and metal rubber densities, where the insertion loss was taken as the evaluation index. The results show that reducing he density of metal rubber may effectively increase the vibration attenuation effectiveness of the pipelines. At the same time, the effect of the ambient temperature on the metal rubber coated damping structure is negligible, indicating that the coated damping structure may work well in a high temperature environment. [ABSTRACT FROM AUTHOR]

Purpose: The prime objective of the present study was to investigate the anticancer properties of angustifoline against COLO-205 human colon cancer cells. Its effects on cell autophagy, apoptosis, cell invasion and cell migration, and cell cycle arrest were also evaluated in the current study., Methods: WST-1 assay was used to study cytotoxic effects of the compound on the cell viability. Effects on apoptosis and cell cycle arrest were evaluated by flow cytometry. In vitro wound healing assay and matrigel assay were carried out to study the effects of angustifoline on cell migration and cell invasion respectively. To confirm autophagy, we evaluated the expression of several autophagy-associated proteins using Western blot assay along with transmission electron microscopy (TEM)., Results: The findings indicated that angustifoline induced dose- and time-dependent cytotoxicity in COLO-205 human colon cancer cells along with inhibiting cancer cell colony formation. Angustifoline-treated cells exhibited cell shrinkage along with distortion of the normal cell morphology. Angustifoline-treated cells were also arrested in the G2/M phase of the cell cycle, showing strong dose-dependence. The compound also led to inhibition of cell migration and cell invasion. The results showed that treatment of these cells led to generation of autophagic cell vesicles. Furthermore, it was observed that the expression of Beclin-1 and LC3-II proteins was significantly upregulated in the angustifoline-administered COLO-205 cells., Conclusions: In brief, the present study hints towards the potent anticancer potential of the natural product angustifoline against COLO-205 human colon cancer cells with in depth mechanistic studies.