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12. The percentage of CD133+ cells in human colorectal cancer cell lines is influenced by Mycoplasma hyorhinis infection

Author

Mirabelli Peppino, Gemei Marica, Mariotti Elisabetta, D'Alessio Francesca, Di Noto Rosa, Fortunato Giuliana, and Del Vecchio Luigi

Subjects
Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
Abstract

Abstract Background Mollicutes contamination is recognized to be a critical issue for the cultivation of continuous cell lines. In this work we characterized the effect of Mycoplasma hyorhinis contamination on CD133 expression in human colon cancer cell lines. Methods MycoAlert® and mycoplasma agar culture were used to detect mycoplasma contamination on GEO, SW480 and HT-29 cell lines. Restriction fragment length polymorphism assay was used to determine mycoplasma species. All cellular models were decontaminated by the use of a specific antibiotic panel (Enrofloxacin, Ciprofloxacin, BM Cyclin 1 and 2, Mycoplasma Removal Agent and MycoZap®). The percentage of CD133 positive cells was analyzed by flow cytometry on GEO, SW480 and HT-29 cell lines, before and after Mycoplasma hyorhinis eradication. Results Mycoplasma hyorhinis infected colon cancer cell lines showed an increased percentage of CD133+ cells as compared to the same cell lines rendered mycoplasma-free by effective exposure to antibiotic treatment. The percentage of CD133 positive cells increased again when mycoplasma negative cells were re-infected by Mycoplasma hyorhinis. Conclusions Mycoplasma hyorhinis infection has an important role on the quality of cultured human colon cancer cell lines giving a false positive increase of cancer stem cells fraction characterized by CD133 expression. Possible explanations are (i) the direct involvement of Mycoplasma on CD133 expression or (ii) the selective pressure on a subpopulation of cells characterized by constitutive CD133 expression. In keeping with United Kingdom Coordinating Committee on Cancer Research (UKCCCR) guidelines, the present data indicate the mandatory prerequisite, for investigators involved in human colon cancer research area, of employing mycoplasma-free cell lines in order to avoid the production of non-reproducible or even false data.

Published
2010
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15. Biochimica del pancreas

Author

F. Salvatore, M. Ruoppolo, Rammendola, R. Ammendola, Fbenfenati, F. Benfenati, Pbuono, P. Buono, Fbussolino, F. Bussolino, Casadio, R., Castagnolo, M., Cimino, F., Colonna, G., Dachà A. Daniele, M. Dachà A. Daniele, Della Ragione, F., Esposito, F., Fontana, S., Guarnieri, C., Merlini, G., Messana, I., Nicolini, C., Oliva, A., Orrù, S., Pechkova, E., Riboni, L., Samaja, M., Spina, A., CARSANA, ANTONELLA, DEL VECCHIO, LUIGI, DI NOTO, ROSA, FARAONIO, RAFFAELLA, FORTUNATO, GIULIANA, GROSSO, MICHELA, IZZO, PAOLA, PANE, FABRIZIO, PAOLELLA, GIOVANNI, PUCCI, PIETRO, SACCHETTI, LUCIA, F.Salvatore, M.Ruoppolo, R.Ammendola, F.Benfenati, P.Buono, F.Bussolino, A. Carsana, R. Casadio, M. Castagnolo, F. Cimino, G. Colonna, M. Dachà A. Daniele, F. Della Ragione, L. Del Vecchio, R. Di Noto, F. Esposito, R. Faraonio, S. Fontana, G. Fortunato, M. Grosso, C. Guarnieri, P. Izzo, G. Merlini, I. Messana, C. Nicolini, A. Oliva, S. Orrù, F. Pane, G. Paolella, E. Pechkova, P. Pucci, L. Riboni, L. Sacchetti, M. Samaja, A. Spina, P. Viani, F. Salvatore, F. Salvatore, M. Ruoppolo, Rammendola, R. Ammendola, Fbenfenati, F. Benfenati, Pbuono, P. Buono, Fbussolino, F. Bussolino, Carsana, Antonella, Casadio, R., Castagnolo, M., Cimino, F., Colonna, G., Dachà A. Daniele, M. Dachà A. Daniele, Della Ragione, F., DEL VECCHIO, Luigi, DI NOTO, Rosa, Esposito, F., Faraonio, Raffaella, Fontana, S., Fortunato, Giuliana, Grosso, Michela, Guarnieri, C., Izzo, Paola, Merlini, G., Messana, I., Nicolini, C., Oliva, A., Orrù, S., Pane, Fabrizio, Paolella, Giovanni, Pechkova, E., Pucci, Pietro, Riboni, L., Sacchetti, Lucia, Samaja, M., and Spina, A.

Published
2013

17. Immunophenotyping of human mesenchymal stem cells from amnion of full-term placentas highlights CD13 as a new obesity marker in pregnancy

Author

IAFFALDANO, LAURA, NARDELLI, CARMELA, PASTORE, LUCIO, DI NOTO, ROSA, MARTINELLI, PASQUALE, SACCHETTI, LUCIA, DEL VECCHIO, LUIGI, Raia M., Mariotti E., Ferrigno M., Quaglia F., Iaffaldano, Laura, Raia, M., Nardelli, Carmela, Mariotti, E., Ferrigno, M., Quaglia, F., Pastore, Lucio, DI NOTO, Rosa, Martinelli, Pasquale, Sacchetti, Lucia, and DEL VECCHIO, Luigi

Published
2012

19. Identification and functional characterization of a new mutation leading to defective uptake of LDL-LDLR complex

Author

A. Ruotolo, M. Romano, M. D. Di Taranto, P. Mirabelli, A. Iannuzzi, M. Gentile, D'AGOSTINO, MARIA NICOLETTA, MAROTTA, GENNARO, DI NOTO, ROSA, DEL VECCHIO, LUIGI, RUBBA, PAOLO OSVALDO FEDERICO, FORTUNATO, GIULIANA, Ruotolo, A., Romano, M., Di Taranto, M. D., Mirabelli, P., D'Agostino, MARIA NICOLETTA, Iannuzzi, A., Marotta, Gennaro, Gentile, M., DI NOTO, Rosa, DEL VECCHIO, Luigi, Rubba, PAOLO OSVALDO FEDERICO, and Fortunato, Giuliana

Published
2011

20. Cord blood plasma adiponectin as predictive marker of obesity in newborns

Author

Labruna, Giuseppe, Nardelli, Carmela, Ferrigno, Maddalena, Mariotti, Elisabetta, Capobianco, Valentina, Quaglia, Filomena, Di Noto, Rosa, Del Vecchio, Luigi, Lucio Pastore, Martinelli, Pasquale, Sacchetti, Lucia, Labruna, Giuseppe, Nardelli, Carmela, M., Ferrigno, E., Mariotti, V., Capobianco, F., Quaglia, DI NOTO, Rosa, DEL VECCHIO, Luigi, Pastore, Lucio, Martinelli, Pasquale, and Sacchetti, Lucia

Published
2011

21. An improved method on stimulated T-lymphocytes to functionally characterize novel and known LDLR mutations

Author

Romano M, Di Taranto MD, Mirabelli P, Iannuzzi A, Gentile M, Raia M, D'AGOSTINO, MARIA NICOLETTA, MAROTTA, GENNARO, DI NOTO, ROSA, DEL VECCHIO, LUIGI, RUBBA, PAOLO OSVALDO FEDERICO, FORTUNATO, GIULIANA, Romano, M, DI TARANTO, MARIA DONATA, Mirabelli, P, D'Agostino, MARIA NICOLETTA, Iannuzzi, A, Marotta, Gennaro, Gentile, M, Raia, M, DI NOTO, Rosa, DEL VECCHIO, Luigi, Rubba, PAOLO OSVALDO FEDERICO, and Fortunato, Giuliana

Abstract

The main causes of familial hypercholesterolemia (FH) are mutations in LDL receptor (LDLR) gene. Functional studies are necessary to demonstrate the LDLR function impairment caused by mutations and would be useful as a diagnostic tool if they allow discrimination between FH patients and controls. In order to identify the best method to detect LDLR activity, we compared continuous Epstein-Barr virus (EBV)-transformed B-lymphocytes and mitogen stimulated T-lymphocytes. In addition, we characterized both novel and known mutations in the LDLR gene. T-lymphocytes and EBV-transformed B-lymphocytes were obtained from peripheral blood of 24 FH patients and 24 control subjects. Functional assays were performed by incubation with fluorescent LDL followed by flow cytometry analysis. Residual LDLR activity was calculated normalizing fluorescence for the mean fluorescence of controls. With stimulated T-lymphocytes we obtained a better discrimination capacity between controls and FH patients compared with EBV-transformed B-lymphocytes as demonstrated by receiver operating characteristic (ROC) curve analysis (the areas under the curve are 1.000 and 0.984 respectively; P < 0.0001 both). The characterization of LDLR activity through T-lymphocytes is more simple and faster than the use of EBV-transformed B-lymphocytes and allows a complete discrimination between controls and FH patients. Therefore the evaluation of residual LDLR activity could be helpful not only for mutation characterization but also for diagnostic purposes.

Published
2011

22. Ruolo dell’epigenetica nella gravidanza della donna obesa

Author

M. Ferrigno, V. Capobianco, E. Mariotti, F. Quaglia, NARDELLI, CARMELA, DI NOTO, ROSA, DEL VECCHIO, LUIGI, PASTORE, LUCIO, IAFFALDANO, LAURA, MARTINELLI, PASQUALE, SACCHETTI, LUCIA, M., Ferrigno, V., Capobianco, Nardelli, Carmela, DI NOTO, Rosa, E., Mariotti, DEL VECCHIO, Luigi, Pastore, Lucio, Iaffaldano, Laura, F., Quaglia, Martinelli, Pasquale, and Sacchetti, Lucia

Published
2010

23. Identification and functional characterization of LDLR mutations in familial hypercholesterolemia patients from Southern Italy

Author

Romano M., Di Taranto M. D., D'AGOSTINO, MARIA NICOLETTA, MAROTTA, GENNARO, Gentile M., Abate G., Mirabelli P., DI NOTO, ROSA, DEL VECCHIO, LUIGI, RUBBA, PAOLO OSVALDO FEDERICO, FORTUNATO, GIULIANA, Romano, M., Di Taranto, M. D., D'Agostino, MARIA NICOLETTA, Marotta, Gennaro, Gentile, M., Abate, G., Mirabelli, P., DI NOTO, Rosa, DEL VECCHIO, Luigi, Rubba, PAOLO OSVALDO FEDERICO, and Fortunato, Giuliana

Abstract

OBJECTIVE: Autosomal dominant hypercholesterolemias are due to defects in the LDL receptor (LDLR) gene, in the apolipoprotein B-100 gene or in the proprotein convertase subtilisin/kexin type 9 gene. The aim of this study was to identify and functionally characterize mutations in the LDLR gene that account for most cases of familial hypercholesterolemia (FH). METHODS: We enrolled 56 unrelated patients from Southern Italy with a clinical diagnosis of FH. The mutation screening was performed by direct sequencing of the promoter and the 18 exons of the LDLR gene and by multiplex ligation-dependent probe amplification (MLPA) analysis to search for large rearrangements. RESULTS AND CONCLUSION: We found 5 new mutations, the causative role of which was demonstrated by functional characterization performed by quantification of fluorescent LDL uptake in EBV-transformed B lymphocytes. These results enlarge the spectrum of FH-causative LDLR mutations. Lastly, screening for large rearrangements is highly recommended for the genetic diagnosis of FH.

Published
2010

24. The CEINGE Biobank of biological samples: storage and management

Author

A. Scotto di Frega, M. Schiattarella, M. Romano, P. Mirabelli, DI NOTO, ROSA, CARSANA, ANTONELLA, FRISSO, GIULIA, SALVATORE, FRANCESCO, FORTUNATO, GIULIANA, A., Scotto di Frega, M., Schiattarella, M., Romano, P., Mirabelli, DI NOTO, Rosa, Carsana, Antonella, Frisso, Giulia, Salvatore, Francesco, and Fortunato, Giuliana

Published
2009

31. High Aminopeptidase N/CD13 Levels Characterize Human Amniotic Mesenchymal Stem Cells and Drive Their Increased Adipogenic Potential in Obese Women

Author

Iaffaldano, Laura, primary, Nardelli, Carmela, additional, Raia, Maddalena, additional, Mariotti, Elisabetta, additional, Ferrigno, Maddalena, additional, Quaglia, Filomena, additional, Labruna, Giuseppe, additional, Capobianco, Valentina, additional, Capone, Angela, additional, Maruotti, Giuseppe Maria, additional, Pastore, Lucio, additional, Di Noto, Rosa, additional, Martinelli, Pasquale, additional, Sacchetti, Lucia, additional, and Del Vecchio, Luigi, additional

Published
2013
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33. Chimeric Beta-Defensin Analogs, Including the Novel 3NI Analog, Display Salt-Resistant Antimicrobial Activity and Lack Toxicity in Human Epithelial Cell Lines

Author

Scudiero, Olga, primary, Galdiero, Stefania, additional, Nigro, Ersilia, additional, Del Vecchio, Luigi, additional, Di Noto, Rosa, additional, Cantisani, Marco, additional, Colavita, Irene, additional, Galdiero, Massimiliano, additional, Cassiman, Jean-Jacques, additional, Daniele, Aurora, additional, Pedone, Carlo, additional, and Salvatore, Francesco, additional

Published
2013
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35. Novel Synthetic, Salt-Resistant Analogs of Human Beta-Defensins 1 and 3 Endowed with Enhanced Antimicrobial Activity

Author

Scudiero, Olga, primary, Galdiero, Stefania, additional, Cantisani, Marco, additional, Di Noto, Rosa, additional, Vitiello, Mariateresa, additional, Galdiero, Massimiliano, additional, Naclerio, Gino, additional, Cassiman, Jean-Jacques, additional, Pedone, Carlo, additional, Castaldo, Giuseppe, additional, and Salvatore, Francesco, additional

Published
2010
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40. Extended flow cytometry characterization of normal bone marrow progenitor cells by simultaneous detection of aldehyde dehydrogenase and early hematopoietic antigens: implication for erythroid differentiation studies

Author

Mirabelli, Peppino, primary, Di Noto, Rosa, additional, Lo Pardo, Catia, additional, Morabito, Paolo, additional, Abate, Giovanna, additional, Gorrese, Marisa, additional, Raia, Maddalena, additional, Pascariello, Caterina, additional, Scalia, Giulia, additional, Gemei, Marica, additional, Mariotti, Elisabetta, additional, and Del Vecchio, Luigi, additional

Published
2008
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42. The percentage of CD133+ cells in humancolorectal cancer cell lines is influenced byMycoplasma hyorhinis infection.

Author

Mariotti, Elisabetta, Gemei, Marica, Mirabelli, Peppino, D'Alessio, Francesca, Di Noto, Rosa, Fortunato, Giuliana, and Del Vecchio, Luigi

Subjects
COLON cancer, MYCOPLASMATACEAE, CANCER treatment, CELL lines, CANCER research, BIOTECHNOLOGY
Abstract

Background: Mollicutes contamination is recognized to be a critical issue for the cultivation of continuous cell lines. In this work we characterized the effect of Mycoplasma hyorhinis contamination on CD133 expression in human colon cancer cell lines. Methods: MycoAlert® and mycoplasma agar culture were used to detect mycoplasma contamination on GEO, SW480 and HT-29 cell lines. Restriction fragment length polymorphism assay was used to determine mycoplasma species. All cellular models were decontaminated by the use of a specific antibiotic panel (Enrofloxacin, Ciprofloxacin, BM Cyclin 1 and 2, Mycoplasma Removal Agent and MycoZap®). The percentage of CD133 positive cells was analyzed by flow cytometry on GEO, SW480 and HT-29 cell lines, before and after Mycoplasma hyorhinis eradication. Results: Mycoplasma hyorhinis infected colon cancer cell lines showed an increased percentage of CD133+ cells as compared to the same cell lines rendered mycoplasma-free by effective exposure to antibiotic treatment. The percentage of CD133 positive cells increased again when mycoplasma negative cells were re-infected by Mycoplasma hyorhinis. Conclusions: Mycoplasma hyorhinis infection has an important role on the quality of cultured human colon cancer cell lines giving a false positive increase of cancer stem cells fraction characterized by CD133 expression. Possible explanations are (i) the direct involvement of Mycoplasma on CD133 expression or (ii) the selective pressure on a subpopulation of cells characterized by constitutive CD133 expression. In keeping with United Kingdom Coordinating Committee on Cancer Research (UKCCCR) guidelines, the present data indicate the mandatory prerequisite, for investigators involved in human colon cancer research area, of employing mycoplasma-free cell lines in order to avoid the production of non-reproducible or even false data. [ABSTRACT FROM AUTHOR]

Published
2010
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43. Cytometric Profiling of CD133+Cells in Human Colon Carcinoma Cell Lines Identifies a Common corePhenotype and Cell Type-specific Mosaics

Author

Gemei, Marica, Di Noto, Rosa, Mirabelli, Peppino, and Del Vecchio, Luigi

Abstract

In colorectal cancer, CD133+cells from fresh biopsies proved to be more tumorigenic than their CD133–counterparts. Nevertheless, the function of CD133 protein in tumorigenic cells seems only marginal. Moreover, CD133 expression alone is insufficient to isolate truecancer stem cells, since only 1 out of 262 CD133+cells actually displays stem-cell capacity. Thus, new markers for colorectal cancer stem cells are needed. Here, we show the extensive characterization of CD133+cells in 5 different colon carcinoma continuous cell lines (HT29, HCT116, Caco2, GEO and LS174T), each representing a different maturation level of colorectal cancer cells. Markers associated with stemness, tumorigenesis and metastatic potential were selected. We identified 6 molecules consistently present on CD133+cells: CD9, CD29, CD49b, CD59, CD151, and CD326. By contrast, CD24, CD26, CD54, CD66c, CD81, CD90, CD99, CD112, CD164, CD166, and CD200 showed a discontinuous behavior, which led us to identify cell type-specific surface antigen mosaics. Finally, some antigens, e.g. CD227, indicated the possibility of classifying the CD133+cells into 2 subsets likely exhibiting specific features. This study reports, for the first time, an extended characterization of the CD133+cells in colon carcinoma cell lines and provides a “dictionary” of antigens to be used in colorectal cancer research.

Published
2013
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44. Complete remission in acute myeloid leukaemia with t(8;21) following treatment with G-CSF: flow cytometric analysis of in vivo andin vitro effects on cell maturation.

Author

Ferrara, Felicetto, Di Noto, Rosa, Viola, Assunta, Russo, Consiglia, Boccuni, Piernicola, Costantini, Silvia, Dello Russo, Annamaria, Lo Pardo, Catia, and Del Vecchio, Luigi

Subjects
*ACUTE myeloid leukemia treatment, *GRANULOCYTE-colony stimulating factor, *CHROMOSOMAL translocation, *FLOW cytometry
Abstract

A 75-year-old patient diagnosed as having acute myeloid leukaemia with t(8;21) received G-CSF alone as induction therapy. Complete remission was achieved following 2 weeks of treatment. Flow cytometric analysis, performed by CD45 technique modified by the introduction of preliminary gating with LDS-751, confirmed the disappearance of blast cells along with myeloid maturation. Finally, in vitro studies demonstrated that G-CSF, as compared to other differentiation inducers, was able to induce a striking effect toward neutrophilic differentiation. [ABSTRACT FROM AUTHOR]

Published
1999
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45. MicroRNA-Mediated Gene Regulation Is Different in Placental Tissues From Obese and Not Obese Pregnant Women at Delivery

Author

Ferrigno, Maddalena, Nardelli, Carmela, Iaffaldano, Laura, Quaglia, Filomena, Martinelli, Pasquale, Sacchetti, Lucia, Capobianco, Valentina, Di Noto, Rosa, Mariotti, Elisabetta, Del Vecchio, Luigi, Lucio Pastore, M., Ferrigno, Nardelli, Carmela, Iaffaldano, Laura, F., Quaglia, Martinelli, Pasquale, Sacchetti, Lucia, V., Capobianco, DI NOTO, Rosa, E., Mariotti, DEL VECCHIO, Luigi, and Pastore, Lucio

46. 683.Adenoviral Vector-Mediated Expression of Bone Morphogenetic Protein-4 in Mesenchymal Stem Cells Induces Differentiation into the Osteoblastic Lineage and Bone Production In Vivo

Author

Lombardo, Barbara, Di Noto, Rosa, Esposito, Maria Teresa, Battista, Sabrina, Ciapetti, Gabriela, Capitani, Ombretta, Nicolais, Luigi, Netti, Paolo, Salvatore, Francesco, and Pastore, Lucio

Subjects
*BONE regeneration, *MESENCHYMAL stem cells
Abstract

An abstract of the article "Adenoviral Vector-Mediated Expression of Bone Morphogenetic Protein-4 in Mesenchymal Stem Cells Induces Differentiation into the Osteoblastic Lineage and Bone Production In Vivo," by Barbara Lombardo and colleagues is presented.

Published
2005
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47. Solid-phase synthesis and pharmacological evaluation of novel nucleoside-tethered dinuclear platinum(II) complexes

Author

Francesco Salvatore, Francesca D'Alessio, Jussara Amato, Francesco Ruffo, Nicola Borbone, Stefano D'Errico, Valentina D'Atri, Giorgia Oliviero, Gennaro Piccialli, Rosa Di Noto, Vincenzo Piccialli, D'Errico, Stefano, Oliviero, Giorgia, Piccialli, Vincenzo, Amato, Jussara, Borbone, Nicola, D'Atri, Valentina, D'Alessio, F, DI NOTO, Rosa, Ruffo, Francesco, Salvatore, Francesco, and Piccialli, Gennaro

Subjects
Antimetabolites, Antineoplastic, Stereochemistry, Clinical Biochemistry, Pharmaceutical Science, chemistry.chemical_element, Antineoplastic Agents, Ethylenediamine, nucleoside, Diamines, Biochemistry, Dinuclear platinum complexe, Inhibitory Concentration 50, Structure-Activity Relationship, chemistry.chemical_compound, Solid-phase synthesis, Hexylamine, Cell Line, Tumor, Diamine, Drug Discovery, Tumor Cells, Cultured, medicine, Humans, Inosine, Molecular Biology, Solid-Phase Synthesis Techniques, Solid-phase synthesi, Aged, Platinum, Molecular Structure, Chemistry, Organic Chemistry, Nucleosides, antitumor compounds, Drug Design, Molecular Medicine, Female, Amine gas treating, Drug Screening Assays, Antitumor, Nucleoside, HeLa Cells, medicine.drug
Abstract

Three novel inosine-based dinuclear platinum complexes have been synthesized via a solid-phase strategy. In these compounds, the metal is linked both to the N-7 of the purine nucleus and to the terminal amine group of a hexylamine side chain installed on N-1. Cis- or trans- diamine as well as ethylenediamine ligands are coordinated to platinum along with a chloride. The synthesised complexes were tested against four different human tumor cell lines. One of these complexes proved to be more cytotoxic than cisplatin against the MCF7 cancer cell line in a short-term exposure assay.

Published
2011
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48. Culture Conditions Allow Selection of Different Mesenchymal Progenitors from Adult Mouse Bone Marrow

Author

Rosa Di Noto, Peppino Mirabelli, Maria Teresa Esposito, Luigi Del Vecchio, Silvia Parisi, Donatella Montanaro, Lucio Pastore, Marisa Gorrese, M. T., Esposito, DI NOTO, Rosa, P., Mirabelli, M., Gorrese, Parisi, Silvia, D., Montanaro, DEL VECCHIO, Luigi, and Pastore, Lucio

Subjects
Homeobox protein NANOG, Aging, Stromal cell, Biomedical Engineering, Clinical uses of mesenchymal stem cells, Bone Marrow Cells, Bioengineering, Biology, Leukemia Inhibitory Factor, Biochemistry, Biomaterials, Mice, Osteogenesis, medicine, Animals, Cell Shape, Cells, Cultured, Cell Proliferation, Stem cell transplantation for articular cartilage repair, Homeodomain Proteins, Adipogenesis, Mesenchymal stem cell, Mesenchymal Stem Cells, Nanog Homeobox Protein, Cell biology, Mice, Inbred C57BL, medicine.anatomical_structure, Culture Media, Conditioned, Immunology, NIH 3T3 Cells, Bone marrow, Stem cell, Chondrogenesis, Adult stem cell
Abstract

The use of adult stem cells in tissue engineering approaches will benefit from the establishment of culture conditions that allow the expansion and maintenance of cells with stem cell–like activity and high differentiation potential. In the field of adult stem cells, bone marrow stromal cells (BMSCs) are promising candidates. In the present study, we define, for the first time, conditions for optimizing the yields of cultures enriched for specific progenitors of bone marrow. Using four distinct culture conditions, supernatants from culture of bone fragments, marrow stroma cell line MS-5, embryonic fibroblast cell line NIH3T3, and a cocktail of epidermal growth factor (EGF) and platelet-derived growth factor (PDGF), we isolated four different sub-populations of murine BMSCs (mBMSCs). These cells express a well-known marker of undifferentiated embryonic stem cells (Nanog) and show interesting features in immunophenotype, self-renewal ability, and differentiation potency. In particular, using NIH3T3 conditioned medium, we obtained cells that showed impairment in osteogenic and chondrogenic differentiation while retaining high adipogenic potential during passages. Our results indicate that the choice of the medium used for isolation and expansion of mBMSCs is important for enriching the culture of desired specific progenitors.

Published
2009
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49. Divergent expression of CD133 in different studies on HCT-116 cell line

Author

Luigi Del Vecchio, Giuliana Fortunato, Marica Gemei, Rosa Di Noto, Peppino Mirabelli, Gemei, M., Mirabelli, P., DI NOTO, Rosa, Fortunato, Giuliana, and DEL VECCHIO, Luigi

Subjects
Cancer Research, Text mining, Oncology, Established cell line, Expression (architecture), Cell culture, business.industry, Immunology, Biology, business, Cell biology
Published
2010
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50. Complete remission in acute myeloid leukaemia with t(8;21) following treatment with G-CSF: flow cytometric analysis of in vivo and in vitro effects on cell maturation

Author

Rosa Di Noto, Annamaria Dello Russo, Consiglia Russo, Felicetto Ferrara, Silvia Costantini, Assunta Viola, Catia Lo Pardo, P Boccuni, Luigi Del Vecchio, Ferrara, F., DI NOTO, Rosa, Viola, A., Russo, C., Boccuni, P., Costantini, S., Dello Russo, A., Lo Pardo, C., and DEL VECCHIO, Luigi

Subjects
Male, Myeloid, Chromosomes, Human, Pair 21, Cellular differentiation, medicine.medical_treatment, Biology, Cell Maturation, Translocation, Genetic, Flow cytometry, In vivo, hemic and lymphatic diseases, Granulocyte Colony-Stimulating Factor, medicine, Humans, Aged, medicine.diagnostic_test, Hematology, Flow Cytometry, medicine.disease, Granulocyte colony-stimulating factor, Leukemia, Treatment Outcome, medicine.anatomical_structure, Cytokine, Leukemia, Myeloid, Acute Disease, Immunology, Cell Division, Chromosomes, Human, Pair 8
Abstract

A 75-year-old patient diagnosed as having acute myeloid leukaemia with t(8;21) received G-CSF alone as induction therapy. Complete remission was achieved following 2 weeks of treatment. Flow cytometric analysis, performed by CD45 technique modified by the introduction of preliminary gating with LDS-751, confirmed the disappearance of blast cells along with myeloid maturation. Finally, in vitro studies demonstrated that G-CSF, as compared to other differentiation inducers, was able to induce a striking effect toward neutrophilic differentiation.

Published
1999
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