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As chronic wasting disease (CWD) continues to spread across North America, the relationship between CWD and host genetics has become of interest. In Rocky Mountain elk (Cervus elaphus nelsoni), one or two copies of a leucine allele at codon 132 of the prion protein gene (132L*) has been shown to prolong the incubation period of CWD. Our study examined the relationship between CWD epidemiology and codon 132 evolution in elk from Wyoming, USA, from 2011 to 2018. Using PCR and Sanger sequencing, we genotyped 997 elk and assessed the relationship between genotype and CWD prevalence estimated from surveillance data. Using logistic regression, we showed that each 1% increase in CWD prevalence is associated with a 9.6% increase in the odds that an elk would have at least one copy of leucine at codon 132. In some regions, however, 132L* variants were found in the absence of CWD, indicating that evolutionary and epidemiologic patterns can be heterogeneous across space and time. We also provide evidence that naturally occurring CWD is not rare in 132L* elk, which merits the study of shedding kinetics in 132L* elk and the influence of genotype on CWD strain diversity. The management implications of cervid adaptations to CWD are difficult to predict. Studies that investigate the degree to which evolutionary outcomes are shaped by host spatial structure can provide useful epidemiologic insight, which can in turn aid management by informing scale and extent of mitigation actions.

Quality deer management (QDM) is the preeminent framework by which white-tailed deer are managed in the eastern USA. Historic differences surrounding the cultures, traditions, and regulations of deer hunting exist regionally between the Midwest and Southeast, yet no published study has investigated regional differences in attitudes and opinions of QDM among deer hunters. We surveyed 9,301 deer hunters across two Midwestern states (Missouri and Ohio) and two Southeastern states (Louisiana and South Carolina) and found that hunters across regions held similar motivations for deer hunting, as well as similar attitudes and opinions of strategies and expected outcomes of QDM. However, there existed several mismatches between the practices and ideology associated with QDM among Southeastern hunters. Southeastern hunters held greater support for greater antlered bag limits, protection of does, and supplemental feeding. We postulate that these mismatches are largely driven by regional differences in cultures, traditions, and regulations surrounding deer hunting. [ABSTRACT FROM AUTHOR]

Archaeological data confirm the widespread use of horse tack throughout the North European Bronze Age and the succeeding Hallstatt period in an inventory that included metal cheekpieces, phalerae, rein-knobs and other horse-related accessories. Similar usage has been assumed in the region of northern Poland, which has also furnished evidence for the use of horse gear accessories made of antler. This paper reports the results of traceological, isotopic and experimental research on three antler cheekpieces found at the Early Iron Age stronghold of Gzin in northern Poland and relates them further with archaeological and ethnographic patterns of horse exploitation. The results indicate that the artefacts from Gzin have rare comparanda and are isotopically consistent with the δ18Ow range of the site location, which can both be taken as evidence of their local origin. Moreover, traceological and experimental evidence from this study places the function and use of the analysed objects into a new light, undermining their use for horseback riding and suggesting that they were employed as cheekpiece coverings. Combined with the ethnographic record, ceramic iconography and archaeolinguistics, our findings fuel a discussion of deer valorisation during the Lusatian period in Poland, showing that the cheekpieces from Gzin may have served as elements of deer headgear. [ABSTRACT FROM AUTHOR]

The study of specimens of Lipoptena fortisetosa Maa, 1965 from various populations of its modern range revealed the presence of two forms that differ morphologically and genetically. Form A corresponds to the original description; form B is distinguished by the presence of a prescutellar suture and 4–5 acrostichal, 4–6 postalar setae. We hypothesise that initially these forms were associated with different hosts and were separated geographically. The L. fortisetosa species was introduced to Europe at least twice from different places and with different hosts. Later, both forms were able to move to other hosts. Now form A lives in Western Europe, Kazakhstan, and the south of the Russian Far East, and form B lives in Eastern Europe, the Sayan Mountains and the Baikal region. [ABSTRACT FROM AUTHOR]

Supplemental feeding of wild ungulates has long been and remains a common practice across Europe and North America. Yet by drawing animals together, supplemental feeding can have unintended, negative effects on individual species and broader ecological processes. These include increased risk of disease transmission, intraspecific and interspecific competition, and predation, which are of management concern for white‐tailed deer (Odocoileus virginianus) in the southeastern United States given the arrival of nonnative wild pigs (Sus scrofa) and coyotes (Canis latrans). We conducted a field experiment between March and July of 2021 to assess the effects of supplemental feeding on spatiotemporal activity patterns of deer and wild pigs at wildlife feeders, and space use of coyotes in the Piedmont region of South Carolina, USA. We observed support for our hypothesis that interspecific competition through increased visitation by larger groups of competitor species reduces use of foraging sites by other subordinate ungulates, where feeders highly visited by wild pigs were rarely visited by deer. While adult deer and wild pigs generally did not shift their temporal activity patterns at feeders, juvenile temporal activity shifted to more frequent visits of feeders during the night, supporting our hypothesis that supplemental feed could increase risk to predator exposure, as coyotes tend to be active during crepuscular hours. Our findings suggest that supplemental feed put out to encourage deer activity could actually deter deer if wild pigs occupy that area, and has potential negative demographic effects if juveniles are at increased risk of predation. Collectively, based on our data, we do not recommend supplemental feeding in the southeastern United States where white‐tailed deer, coyotes, and wild pigs co‐occur. More broadly, given how widespread the legal use of supplemental feed remains across the United States, we encourage landowners and policymakers to consider the full suite of potential direct and indirect, short‐term and long‐term negative impacts supplemental feeding can have on both target and nontarget wildlife populations. [ABSTRACT FROM AUTHOR]

Lead (Pb) is a heavy metal with no clear biological benefit that is toxic to wildlife and humans. Although Pb has been banned from several consumer products, it is not regulated in ammunition for big‐game hunting (e.g., cervids) in New York, USA. Wildlife species that scavenge remains of game harvested with Pb ammunition can be exposed to bullet fragments through ingestion and potentially develop toxicosis. To make evidence‐based decisions for reducing risk from bullet‐derived Pb, we first empirically identified avian and mammalian scavengers of white‐tailed deer (Odocoileus virginianus) remains in New York through an online survey during July‐August 2023, where community members submitted images of scavengers from game cameras. We conducted species vulnerability and utility assessments using published evidence and pre‐existing frameworks. Despite inherent limitations, we documented an extensive array of avian (n = 17) and mammalian (n = 14) scavengers. We estimated bald eagles (Haliaeetus leucocephalus) and golden eagles (Aquila chrysaetos) as most vulnerable to Pb toxicosis, followed collectively by American goshawks (Accipiter atricapillus), American crows (Corvus brachyrhynchos), and common ravens (C. corax). We also determined bald eagles to be best‐suited as bioindicators of bullet‐derived Pb, followed by American goshawks. Red‐tailed hawks (Buteo jamaicensis) and great horned owls (Bubo virginianus) equally ranked third. Integrating vulnerability and utility assessments point to bald eagles as the most vulnerable scavengers to Pb toxicosis and best‐suited bioindicators of bullet‐derived Pb. Because regulation of Pb ammunition for big‐game hunting is an ongoing socio‐political issue, wildlife managers and conservationists can address health risks through monitoring species vulnerable to toxicosis and instituting surveillance programs using the most effective bioindicators. [ABSTRACT FROM AUTHOR]

Farmed cervids are of growing economic importance in the midwestern United States. Although diseases of wild and captive cervids have been examined in more northerly climates, little information exists on the health challenges of deer in the Midwest. We characterized and summarized the causes of mortality in farmed white-tailed deer (Odocoileus virginianus) submitted to the University of Missouri Veterinary Medical Diagnostic Laboratory (Columbia, MO, USA) over a 19-y period (2004–2023). Of 388 cases examined, 253 (65%) were carcasses submitted for autopsy, and 135 (35%) cases were tissue samples harvested by field veterinarians. Infectious disease was the most common cause of mortality (n = 335; 86.3%). Of infectious causes, primary pneumonia was most common (n = 140; 41.7%), followed by septicemia (n = 68; 20.1%), and primary enteritis (n = 64; 19.1%). Viruses were detected in 18% of pneumonia cases. The most common non-infectious diagnoses were emaciation and trauma (both 4 each; 1%). Thirteen animals (3.4%) died of unknown causes. Forty-nine percent of cases were juvenile deer <1-y-old. Most cases were received in the summer (212; 54.6%). Infectious diseases, particularly bacteria and viruses, pose a significant health challenge to farmed deer in the midwestern United States. [ABSTRACT FROM AUTHOR]

Investigating the relationship between the physical environment and organismal adaptation is crucial to understanding the selective pressures that drive mammalian evolution. Of the varied approaches to exploring this relationship, macroscopic tooth wear analyses have become widely used due to their economic and simple methodologies. However, more work is needed to understand how assessments of wear patterns operate within finer levels of dietary and environmental contexts. It is specifically unclear if and how tooth wear differs inter‐ and intraspecifically between geographically widespread, large‐bodied mammalian populations. This project characterizes molar hypsodonty indices and mesowear scores of white‐tailed deer (Odocoileus virginianus) and mule deer (Odocoileus hemionus) populations across North and Central America, investigating if and how molar wear and morphology vary over the deer's large and ecologically variable geographic distribution. While mesowear scores generally do not strongly track environmental signals within and between Odocoileus species, hypsodonty indices reveal a strong correlation between crown height and aridity, which likely represents an adaptive response to abrasive diets in areas with low precipitation. [ABSTRACT FROM AUTHOR]

Chronic wasting disease (CWD) is a widely distributed prion disease of cervids with implications for wildlife conservation and also for human and livestock health. The structures of infectious prions that cause CWD and other natural prion diseases of mammalian hosts have been poorly understood. Here we report a 2.8 Å resolution cryogenic electron microscopy-based structure of CWD prion fibrils from the brain of a naturally infected white-tailed deer expressing the most common wild-type PrP sequence. Like recently solved rodent-adapted scrapie prion fibrils, our atomic model of CWD fibrils contains single stacks of PrP molecules forming parallel in-register intermolecular β-sheets and intervening loops comprising major N- and C-terminal lobes within the fibril cross-section. However, CWD fibrils from a natural cervid host differ markedly from the rodent structures in many other features, including a ~ 180° twist in the relative orientation of the lobes. This CWD structure suggests mechanisms underlying the apparent CWD transmission barrier to humans and should facilitate more rational approaches to the development of CWD vaccines and therapeutics. [ABSTRACT FROM AUTHOR]

Woodland caribou (Rangifer tarandus caribou) are declining across much of their distribution in Canada in response to habitat alteration, leading to unsustainable predation, particularly by wolves (Canis lupus). Habitat alteration can benefit the primary prey species of wolves (moose [Alces alces] and deer [Odocoileus spp.]) by creating early seral conditions that contain more of their preferred food types. This increase in primary prey populations results in elevated wolf abundance and heightened predation pressure on caribou. In response to the elevated wolf populations and the risks to caribou, managers have reduced wolf abundance in key areas. Ecological theory suggests that reducing wolf abundance would release moose from the top‐down effects of wolf predation, potentially allowing moose populations to grow. Elevated moose abundance thus has the potential to cause wolf populations to rebound quickly each year following reductions, suggesting a possible link between moose abundance and the number of wolves killed for caribou conservation. To test this idea we used a unique management situation in British Columbia and Alberta, Canada, where lethal wolf removals were annually conducted across specific southern mountain caribou population ranges and, in some places, moose populations were concurrently reduced via liberalized hunting. We used indices of moose abundance and wolf removal data to test the hypothesis that reducing moose populations to a historical abundance target by hunting leads to fewer wolves killed for caribou conservation. After controlling for habitat quality, wolves removed per km2 was 3.2 times lower in areas with reduced moose density ( x ¯ $\bar{x}$ = 1.55 wolves/1,000 km2 ± 0.33 [SE]) than in those without reduced moose density ( x ¯ $\bar{x}$ = 5.02 wolves/1,000 km2 ± 0.52). However, the average number of wolves removed per year decreased under both conditions. After 9 years, there was a 35% reduction in the predicted difference in the annual removal between areas with and without moose reduction. Our results suggest that policies that do not reduce or stabilize moose abundance will result in the removal of more wolves to increase caribou abundance. Like wolf reductions, moose reductions can also be controversial and affect local harvesters. Thus, understanding the consequences of actions that support caribou recovery is essential to supporting evidence‐based policy discussions. [ABSTRACT FROM AUTHOR]

Neurotransmitter:sodium symporters (NSSs) play critical roles in neural signaling by regulating neurotransmitter uptake into cells powered by sodium electrochemical gradients. Bacterial NSSs orthologs, including MhsT from Bacillus halodurans, have emerged as model systems to understand the structural motifs of alternating access in NSSs and the extent of conservation of these motifs across the family. Here, we apply a computational/experimental methodology to illuminate the conformational landscape of MhsT alternating access. Capitalizing on our recently developed method, Sampling Protein Ensembles and Conformational Heterogeneity with AlphaFold2 (SPEACH_ AF), we derived clusters of MhsT models spanning the transition from inward-facing to outward-facing conformations. Systematic application of double electron-electron resonance (DEER) spectroscopy revealed ligand-dependent movements of multiple structural motifs that underpin MhsT's conformational cycle. Remarkably, comparative DEER analysis in detergent micelles and lipid nanodiscs highlights the profound effect of the environment on the energetics of conformational changes. Through experimentally derived selection of collective variables, we present a model of ion and substrate-powered transport by MhsT consistent with the conformational cycle derived from DEER. Our findings not only advance the understanding of MhsT's function but also uncover motifs of conformational dynamics conserved within the broader context of the NSS family and within the LeuT-fold class of transporters. Importantly, our methodological blueprint introduces an approach that can be applied across a diverse spectrum of transporters to describe their conformational landscapes. [ABSTRACT FROM AUTHOR]

Background: Early detection and monitoring of SARS-CoV-2 infections in animal populations living in close proximity to humans is crucial for preventing reverse zoonosis of new viral strains. Evidence accumulated has revealed widespread SARS-CoV-2 infection among white-tailed deer (WTD), (Odocoileus virginianus) populations in the United States except in the southeast region. Therefore, the objective was to conduct surveillance for evidence of SARS-CoV-2 infection among WTD in Mississippi. Materials and Methods: Blood, kidney tissues, and nasal swab samples were collected in 17 counties from hunter-harvested deer during 2021-2022 and 2022-2023.Samples of kidney tissue were collected to evaluate for detecting antibody as a possible alternative to blood that is not always available from dead WTD. Nasal swab samples were tested for SARS-CoV-2 viral RNA by a RT-PCR assay. Sera and kidney tissue samples were tested for SARS-CoV-2 antibody by an enzyme-linked immunoassay (ELISA) and sera by a plaque reduction neutralization test (PRNT80). Results: The results of testing sera and kidney homogenate samples provided the first evidence of SARS-CoV-2 infection among WTD in Mississippi. The infection rate during 2021–2022 was 67% (10/15) based on the detection of neutralizing antibody by the PRNT80 and 26%(16/62) based on the testing of kidney tissue homogenates by an ELISA, and viral RNA was detected in 25% (3/12) of nasal swab samples. In 2022 to 2023, neutralizing antibody was detected in 62% (28/45) of WTD serum samples. In contrast, antibodies were not detected in 220 kidney homogenates by an ELISA nor was viral RNA detected in 220 nasal swab samples. Evidence of WTD activity was common in urban areas during the survey. Conclusion: Overall, the findings documented the first SARS-CoV-2 infection among WTD in Mississippi and showed that WTD commonly inhabited urban areas as a possible source of acquiring infection from humans infected with this virus. [ABSTRACT FROM AUTHOR]

To protect wild deer populations (and their beneficiaries) from the negative consequences of chronic wasting disease (CWD), it is crucial to engage deer hunters in disease prevention. Early qualitative findings suggest CWD-relevant behaviors evoke moral or ethical considerations in deer hunters which may help to explain behaviors. The present study applies norm-activation theory (NAT) to explore the relationship between personal norms and CWD-preventative behavioral intentions. We surveyed 2,000 New York, USA residents licensed to hunt in the bordering CWD-positive state of Pennsylvania, USA, and find most hunters have activated personal norms to help prevent a CWD (re)introduction to New York. We find as perceived effectiveness of the behaviors (in helping to prevent CWD) increases, the odds of activated personal norms increase, as do intentions to perform the behaviors. Our findings offer insights into deer hunter involvement in CWD prevention and the potential influence of internal motivation on the respective behaviors. [ABSTRACT FROM AUTHOR]

Background: Tens of thousands of deer are hit by cars in the UK each year. Many die, many immediately run away despite injury, but some are incapacitated and a vet may be called to the animal by the driver, a passer‐by or the police. Less often a deer is brought to a veterinary practice. Occasionally, vets may be asked to attend deer trapped in fencing or incapacitated in other ways. As more rural practices have come to serve companion animals only, and more wild deer have been moving into suburban and even urban environments, companion animal vets unfamiliar with deer have occasionally found themselves having to deal with these species. Aim of the article: This article provides relevant information for situations where companion animal vets are tasked with dealing with injured wild deer, including legal, practical, ethical and welfare issues. [ABSTRACT FROM AUTHOR]

Overabundant ungulate populations are frequently hard to control in woodlands and protected areas, such as El Palmar National Park, where controlled shooting failed to reduce the relative abundance of axis deer (Axis axis) over a decade. We compared the effectiveness of spotlight hunting to cull axis deer with daytime and nighttime controlled shooting over bait stations. We tested whether harvest composition differed among methods by multiple regression and fitted functional response models to culling and sighting data. The trial included 18 sessions and 49 hunter groups, each supervised by a park ranger, who traveled a randomly allocated, fixed itinerary in a slow-moving vehicle, sighted and shot the deer, and retrieved and measured the carcass. Spotlighting culled 2.8–6.2 times more deer per hunting party-session and a significantly greater fraction of subadult or adult deer than daytime and overnight shooting. Relatively more females were culled by spotlighting (58.5%) than by daytime (43.5%) and overnight (47.3%) sessions. Pregnancy rates were 2.4–3.8 times greater by spotlighting (34.0%) than in overnight (14.1%) or daytime (9.0%) sessions. The deer harvested by spotlighting or overnight shooting were significantly heavier than those culled by daytime sessions. A Type II functional response fitted the data better than other competing models. Inter-method differences in harvest size and composition most likely explain the historical inability of daytime shooting to curb deer numbers despite intensified efforts. Spotlighting conducted by supervised, organized hunters was effective for targeted deer control and removed deer with greater reproductive value than shooting over bait stations. [ABSTRACT FROM AUTHOR]

This paper reports the activity concentrations of 137Cs, 90Sr, 239+240Pu, 241Am, and 3Н in the form of tritiated water (НТО) and organically bound tritium (ОBТ) in the tissues and organs of roe deer (Capreolus pygargus Pal., 1771) that inhabit the 'Degelen' test location of the Semipalatinsk Test Site. Tissues and organs were sampled from six deer by killing. The activity concentrations of specific radionuclides in the samples were measured using γ-, α-, and β-spectrometry. The radionuclide activity concentrations in the tissues and organs showed considerable variation, for example, 0.6–170 Bq kg-1 for 137Cs and 0.3–2.8×103 Bq kg-1 for 90Sr. The activity concentrations of radionuclides in animal muscular tissue did not exceed permissible values for the meat of wild animals. The tissues and organs in the roe deer were arranged as follows in descending order of their ability to accumulate 137Cs and 90Sr: for 137Cs, muscular tissue–kidneys–lungs–spleen–heart–liver–bone tissue; for 90Sr, bone tissue–liver–lungs–muscular tissue–spleen–heart–kidneys. The activity concentrations of 241Am and 239+240Pu did not exceed the minimum detectable activity. Therefore, no quantitative values could be determined for 241Am, and the 239+240Pu activity concentration could be derived for only one sample: 0.5±0.1 Bq kg-1 (liver). The distribution pattern of these radionuclides in the tissues and organs of the roe deer could not be determined because of insufficient data. The HTO volumetric activity in the tissues and organs of the examined animals ranged from 2.6×10−2 to 77 kBq l-1; activity concentration of OBT, 3.0×10−2 to 16 kBq kg-1; and OBT-to-HTO ratios, 2.0×10−3 to 5.3×102. This ratio can serve as an indicator of how long the examined animals stay in radioactively contaminated ecosystems. Within the 'Degelen' site, the activity concentrations of 90Sr and tritium, in the form of HTO and OBT, are expected to be high in the bone tissues, soft tissues, and organs, respectively. [ABSTRACT FROM AUTHOR]

Riboswitches control gene regulation upon external stimuli such as environmental factors or ligand binding. The fluoride sensing riboswitch from Thermotoga petrophila is a complex regulatory RNA proposed to be involved in resistance to F− cytotoxicity. The details of structure and dynamics underpinning the regulatory mechanism are currently debated. Here we demonstrate that a combination of pulsed electron paramagnetic resonance (ESR/EPR) spectroscopies, detecting distances in the angstrom to nanometre range, can probe distinct regions of conformational flexibility in this riboswitch. PELDOR (pulsed electron‐electron double resonance) revealed a similar preorganisation of the sensing domain in three forms, i.e. the free aptamer, the Mg2+‐bound apo, and the F−‐bound holo form. 19F ENDOR (electron‐nuclear double resonance) was used to investigate the active site structure of the F−‐bound holo form. Distance distributions without a priori structural information were compared with in silico modelling of spin label conformations based on the crystal structure. While PELDOR, probing the periphery of the RNA fold, revealed conformational flexibility of the RNA backbone, ENDOR indicated low structural heterogeneity at the ligand binding site. Overall, the combination of PELDOR and ENDOR with sub‐angstrom precision gave insight into structural organisation and flexibility of a riboswitch, not easily attainable by other biophysical techniques. [ABSTRACT FROM AUTHOR]

Aims: We investigated the presence of SARS‐CoV‐2 in free‐ranging wildlife populations in Northeastern Minnesota on the Grand Portage Indian Reservation and Isle Royale National Park. Methods and Results: One hundred twenty nasal samples were collected from white‐tailed deer, moose, grey wolves and black bears monitored for conservation efforts during 2022–2023. Samples were tested for viral RNA by RT‐qPCR using the CDC N1/N2 primer set. Our data indicate that no wildlife samples were positive for SARS‐CoV‐2 RNA. Conclusions: Continued surveillance is therefore crucial to better understand the changing landscape of zoonotic SARS‐CoV‐2 in the Upper Midwest. [ABSTRACT FROM AUTHOR]

We surveyed Cudjoe and Sugarloaf keys in Florida to estimate Odocoileus virginianus clavium (Key Deer) numbers using 14 motion-detecting infrared cameras to determine population numbers after deer had been translocated to these keys in 2005. We activated 6 cameras deployed on Sugarloaf Key and 8 cameras on Cudjoe Key on 11 March 2023 and retrieved them on 1 April 2023. We obtained 1340 and 2192 photos of Key Deer on Sugarloaf and Cudjoe keys, respectively. We individually identified 8 males on Sugarloaf Key and 15 males on Cudjoe Key by their antler configuration and considered them "marked" for a Lincoln–Petersen mark–resight population estimate. The Lincoln–Petersen method estimated 36 Key Deer on Sugarloaf Key and 74 Key Deer on Cudjoe Key. These estimates indicate the translocation thus far has been effective, but there is a need to monitor the efficacy of translocations over time to ensure the long-term viability of the species. [ABSTRACT FROM AUTHOR]

High-quality observations of the real world are crucial for a variety of applications, including producing 3D printed replicas of small-scale scenes and conducting inspections of large-scale infrastructure. These 3D observations are commonly obtained by combining multiple sensor measurements from different views. Guiding the selection of suitable views is known as the Next Best View (NBV) planning problem. Most NBV approaches reason about measurements using rigid data structures (e.g., surface meshes or voxel grids). This simplifies next best view selection but can be computationally expensive, reduces real-world fidelity and couples the selection of a next best view with the final data processing. This paper presents the Surface Edge Explorer (SEE), a NBV approach that selects new observations directly from previous sensor measurements without requiring rigid data structures. SEE uses measurement density to propose next best views that increase coverage of insufficiently observed surfaces while avoiding potential occlusions. Statistical results from simulated experiments show that SEE can attain similar or better surface coverage with less observation time and travel distance than evaluated volumetric approaches on both small- and large-scale scenes. Real-world experiments demonstrate SEE autonomously observing a deer statue using a 3D sensor affixed to a robotic arm. [ABSTRACT FROM AUTHOR]

Climate change causes far‐reaching disruption in nature, where tolerance thresholds already have been exceeded for some plants and animals. In the short term, deer may respond to climate through individual physiological and behavioral responses. Over time, individual responses can aggregate to the population level and ultimately lead to evolutionary adaptations. We systematically reviewed the literature (published 2000–2022) to summarize the effect of temperature, rainfall, snow, combined measures (e.g., the North Atlantic Oscillation), and extreme events, on deer species inhabiting boreal and temperate forests in terms of their physiology, spatial use, and population dynamics. We targeted deer species that inhabit relevant biomes in North America, Europe, and Asia: moose, roe deer, wapiti, red deer, sika deer, fallow deer, white‐tailed deer, mule deer, caribou, and reindeer. Our review (218 papers) shows that many deer populations will likely benefit in part from warmer winters, but hotter and drier summers may exceed their physiological tolerances. We found support for deer expressing both morphological, physiological, and behavioral plasticity in response to climate variability. For example, some deer species can limit the effects of harsh weather conditions by modifying habitat use and daily activity patterns, while the physiological responses of female deer can lead to long‐lasting effects on population dynamics. We identified 20 patterns, among which some illustrate antagonistic pathways, suggesting that detrimental effects will cancel out some of the benefits of climate change. Our findings highlight the influence of local variables (e.g., population density and predation) on how deer will respond to climatic conditions. We identified several knowledge gaps, such as studies regarding the potential impact on these animals of extreme weather events, snow type, and wetter autumns. The patterns we have identified in this literature review should help managers understand how populations of deer may be affected by regionally projected futures regarding temperature, rainfall, and snow. [ABSTRACT FROM AUTHOR]

The frequency of severe wildfires is on the rise in the Mediterranean Basin as a result of climate change and land abandonment. Recurrent wildfires may retard or impede ecosystem recovery, frequently requiring the implementation of restoration practices. In that context, a post‐fire deer exclusion experiment was conducted in a Mediterranean mixed forest ecosystem with high population densities of deer species (Fallow deer and Red deer). A deer exclusion area was established as a form of restoration intervention to promote post‐fire vegetation recovery. We surveyed plant species during the second and third springs after fire in both deer‐excluded and deer‐allowed areas in order to detect changes in the community. We analyzed the plant species composition, species diversity, and structure of herbaceous and woody plant communities. We also compared the frequency of annual and perennial herb species, functional groups, and post‐fire plant regeneration strategies. The main differences were due to differences between springs rather than of deer‐excluded and deer‐allowed plots. Deer consumed both woody and herbaceous species, favoring the assemblage of herbaceous over woody species. Deer favored the establishment of annual forbs, including unpalatable and/or exotic species, while limiting the abundance of graminoids. Observed deer impacts could have been magnified during the second spring by a drought. Our study demonstrates that deer exclusion can be an effective restoration practice for promoting post‐fire herbaceous regeneration in forests with significant densities of wild ungulates, although seasonal effects may override restoration practice effects. [ABSTRACT FROM AUTHOR]

Simple Summary: The relationship between the saliva urea N (SUN) and plasma urea N (PUN) concentrations was investigated in red deer calves (n = 23) over a five-month period with the objective of creating an easy tool to quickly evaluate circulating urea nitrogen concentrations. The experimental design comprised a 2 × 2 factorial arrangement with different seasonality phenotypes (high and low) and two different forage-based diets, namely a medium-quality diverse treatment (Diverse) or a low-quality perennial ryegrass–white clover treatment (PRG), which were offered ad libitum. Blood and saliva samples for the determination of the PUN and SUN were evaluated monthly at civil dawn between April and September of 2022. A moderately strong relationship between the PUN and SUN was identified by simple linear regression (R2 = 0.65; p < 0.001). The interaction between diet and the date of sampling had a significant effect on the relationship between the SUN and PUN (p < 0.001). This interaction is likely explained by the seasonal variation in the crude protein (CP) content in the diet. The use of a mixed-model procedure improved the model fit, increasing the R2 by 0.12 and reducing the root mean square error by 0.341. There was no effect of seasonality on the prediction estimates of the PUN from the SUN, although a tendency for a sex–seasonality interaction was observed (p = 0.09). Therefore, an evaluation of the diet and date of sampling can be used to improve the reliability of the prediction estimates of the PUN from the SUN in red deer calves. Further investigation into the effects of significant factors on this relationship is required to improve the reliability of the model before the SUN can be used to predict the PUN. Red deer (Cervus elaphus), like other ruminants, excrete approximately 70% of the nitrogen they ingest. Developing ways in which to reduce the rate of loss, such as manipulating the diet or selecting for efficiency of growth, requires close monitoring of the plasma urea N (PUN) concentration which, in turn, requires a simple, safe, and reliable method for collecting samples. Saliva is easier to collect than blood, but the relationship between the salivary urea N (SUN) and the PUN is not known for red deer. This was therefore evaluated in two strains of mixed-sex red deer calves (Cervus elaphus): a phenotype with a high seasonality of growth (H, n = 10) and a phenotype with a low seasonality of growth (L, n = 13). Both phenotypes were divided into two groups, which were each offered one of two forage-based diets ad libitum: a medium-quality diverse treatment and a low-quality perennial ryegrass–white clover treatment. Blood and saliva samples for the determination of the PUN and SUN were collected at dawn every four weeks for five months (April to September 2022). There was a strong linear relationship between the PUN and SUN in the pooled sample (R2 = 0.65, p < 0.001). The estimations of the PUN were significantly improved by adding diet and the date of sampling into the model (p < 0.001), but not phenotype (p > 0.75). SUN represents a reliable index of the PUN, and collecting saliva therefore represents a simple and inexpensive alternative to collecting blood samples in studies of nitrogen metabolism in red deer. [ABSTRACT FROM AUTHOR]

• The DEHP was detected in serum and hair of M. berezovskii in captivity in some farms. • MA fed female M. berezovskii improved the survival rate of M. berezovskii pups. • DEHP induces oxidative stress and apoptosis of PMCs by reducing PRDX6 expression. • MA prevents DEHP inducing apoptosis via upregulating PRDX6 in PMCs. Chinese forest musk deer (FMD), an endangered species, have exhibited low reproductive rates even in captivity due to stress conditions. Investigation revealed the presence of di(2-ethylhexyl) phthalate (DEHP), an environmental endocrine disruptor, in the serum and skin of captive FMDs. Feeding FMDs with maslinic acid (MA) has been observed to alleviate the stress response and improve reproductive rates, although the precise molecular mechanisms remain unclear. Therefore, this study aims to investigate the molecular mechanisms underlying the alleviation of DEHP-induced oxidative stress and cell apoptosis in primary peritubular myoid cells (PMCs) through MA intake. Primary PMCs were isolated and exposed to DEHP in vitro. The results demonstrated that DEHP significantly suppressed antioxidant levels and promoted cell apoptosis in primary PMCs. Moreover, interfering with the expression of PRDX6 was found to induce excessive reactive oxygen species (ROS) production and cell apoptosis in primary PMCs. Supplementation with MA significantly upregulated the expression of PRDX6, thereby attenuating DEHP-induced oxidative stress and cell apoptosis in primary PMCs. These findings provide a theoretical foundation for mitigating stress levels and enhancing reproductive capacity of in captive FMDs. [Display omitted] [ABSTRACT FROM AUTHOR]

Chronic wasting disease (CWD) is a neurodegenerative disease that threatens deer population health and management. This qualitative study explored processors' perception of CWD risks, and the solutions or barriers they experience regarding managing these risks. Processors can play a critical role preventing consumption of CWD-positive venison by keeping CWD-positive deer out of facilities and implementing practices that minimize the risk of cross-contamination should CWD-positive deer enter facilities. Our analysis of semi-structured interviews of wild game processors found that they are concerned about CWD but have not altered processing practices, believing that most of the responsibilities for preventing CWD spread lie with hunters. Processors suggested several practices that state regulatory agencies can implement to aid in efforts to ensure that no CWD-positive deer enter facilities. This research may inform state agencies' CWD communication efforts and identify actionable policies that better ensure processors can help prevent the consumption of CWD-positive venison. [ABSTRACT FROM AUTHOR]

Globally, collisions with vehicles result in millions of animal deaths every year, representing a major issue for wildlife conservation and management. Consequently, and importantly, much research has focused on understanding patterns of animal–vehicle collisions with the aim to reduce roadkill of wildlife. However, existing data on animal–vehicle collisions might also represent a novel opportunity to monitor wildlife populations. For this purpose, we compared data of >1.2 million hunter‐shot deer and >40,000 deer–vehicle collisions collected over 11 years in Denmark. We show that deer–vehicle collision data can be useful for population monitoring of roe deer (Capreolus capreolus), fallow deer (Dama dama), and red deer (Cervus elaphus). Roe deer was the most numerous species, representing 90% of observations based both on deer–vehicle collisions and on hunting bag statistics. After accounting for factors related to road infrastructure (road length and density, traffic volume), local (municipality) deer–vehicle collisions were highly correlated with hunting bag data for roe and red deer (Pearson's r > 0.7) but not fallow deer, likely due to biases in hunting bags. Moreover, we used deer–vehicle collision data to map spatiotemporal changes in the distribution of fallow and red deer, and demographic changes in all species. Combined, our results suggest that animal–vehicle collision data can be a useful tool to supplement existing methods for monitoring wildlife populations, which will be relevant for the management of these populations. We point to important shortcomings in both animal–vehicle collision and hunting bag data and provide recommendations on how to improve their accuracy in the future, to be applicable for a broader range of species. [ABSTRACT FROM AUTHOR]

Background: HEV is endemic in several Middle Eastern countries including Saudi Arabia, which hosts the annual pilgrimage for Muslims from around the world. One of the Hajj rituals is the sacrifice of animals, including camels, cows, goats, and sheep. HEV Zoonosis is established in swine and other suspected species, including deer, rabbits, dromedary, and Bactrian camels. HEV was identified in small, domesticized animals like goats, cows, sheep, and horses. We previously investigated HEV seroprevalence in Camels. This study aimed to evaluate HEV seroprevalence in other highly consumed ruminants in Saudi Arabia, namely cows, sheep, and goats. Methods: Sera from cows (n = 47), goats (n = 56), and sheep (n = 67) were analyzed for the presence of HEV-IgG by using in-house developed ELISA assays. Results: The highest seroprevalence was found in sheep (62.7%), followed by cows (38.3%), and then goats (14.3%), with a p-value of < 0.001. No other demographic characteristics of the animals were significantly correlated with the HEV seroprevalence. Conclusions: This study provides baseline data as the first study on the seroprevalence of HEV in ruminant animals in Saudi Arabia. The high seroprevalence found in sheep and cows must be further investigated for the potential zoonotic HEV transmission to humans. Further studies are needed to investigate the active viremia in these animal species through nucleic acid detection and sequencing to provide data on the circulating HEV genotypes among the targeted animal species. The detection of HEV in different animal products, such as milk, liver, and others, also remains an important study area to consider. [ABSTRACT FROM AUTHOR]

Abstract The structure of cellular prion proteins encoded by the prion protein gene (PRNP) impacts susceptibility to transmissible spongiform encephalopathies, including chronic wasting disease (CWD) in deer. The recent emergence of CWD in Northern European reindeer (Rangifer tarandus), moose (Alces alces alces) and red deer (Cervus elaphus), in parallel with the outbreak in North America, gives reason to investigate PRNP variation in European deer, to implement risk assessments and adjust CWD management for deer populations under threat. We here report PRNP-sequence data from 911 samples of German red, roe (Capreolus capreolus), sika (Cervus nippon) and fallow deer (Dama dama) as well as additional data from 26 Danish red deer close to the German border and four zoo species not native to Germany. No PRNP sequence variation was observed in roe and fallow deer, as previously described for populations across Europe. In contrast, a broad PRNP variation was detected in red deer, with non-synonymous polymorphisms at codons 98, 226 and 247 as well as synonymous mutations at codons 21, 78, 136 and 185. Moreover, a novel 24 bp deletion within the octapeptide repeat was detected. In summary, 14 genotypes were seen in red deer with significant differences in their geographical distribution and frequencies, including geographical clustering of certain genotypes, suggesting “PRNP-linages” in this species. Based on data from North American CWD and the genotyping results of the European CWD cases, we would predict that large proportions of wild cervids in Europe might be susceptible to CWD once introduced to naive populations.

Deer production options were modelled in two contrasting environments, Otago and Hawkes Bay, New Zealand, to estimate greenhouse gas emissions, productivity and profitability. A standard New Zealand deer farm was compared to the deer production scenarios: (1) increasing hind longevity through improved reproductive performance, (2) improving animal health management to increase weaner growth, (3) pre-winter finishing using larger hinds and superior growth genetics sires or (4) shifting to a mixed velvet antler/venison enterprise. Production (kg product/ha) increased as scenarios 1–3 were applied, being greatest for scenario 3, in both environments (+21% and +2% in the Otago and Hawkes Bay environments respectively). Production increases in the Hawkes Bay environment were muted by the inclusion of beef and sheep enterprises. Production in Otago and Hawkes Bay was reduced in scenario 4 by 12 and 7% but achieved increased gross margin (33% and 20% respectively). Some small differences in the magnitude of response were seen between the environments. Changes in total GHG emissions per hectare ranged between +4.6% and −6.7%. GHG emission per $ gross margin was reduced by up to 15%–21%. Altering production options in the deer industry altered total GHG emissions per hectare and reduced GHG emissions per kg of product. [ABSTRACT FROM AUTHOR]

Under an optimal foraging scenario, prey selection would be expected to occur when food resources are abundant. A positive frequency‐dependent prey selection would elicit prey switching when the abundance of main food resources decreases, potentially favouring community resilience to the effects of intensive, selective predation on a single prey. We assessed whether a positive frequency‐dependent prey selection by the wolf Canis lupus occurred in two areas hosting abundant populations of wild ungulates, one in northern (Słowiński National Park, Poland) and the other one in southern (Maremma Regional Park, Italy) Europe, throughout a cold semester. In Słowiński, ungulate community was dominated by red deer Cervus elaphus (57% availability) over wild boar Sus scrofa (35%) and roe deer Capreolus capreolus (8%); wild boar and fallow deer Dama dama (43–37%) were more abundant than roe deer (20%) in Maremma. In both areas, wolf diet was dominated by wild ungulates, with a major use of red deer in Słowiński and wild boar in Maremma. Prey selection occurred in both areas, and it was addressed towards the most abundant prey in Słowiński, that is, the red deer, but only towards the wild boar in Maremma, where the fallow deer was used according to availability. In Slowinski, high red deer density may have driven wolf prey selection, while the shifting of activity rhythms of the fallow deer in the Maremma as antipredator response to wolf presence may have reduced predation. Despite its comparable densities between the two areas, the wild boar was selected in Maremma and under‐used in Słowiński. Results provide partial support to positive frequency‐dependent selection, emphasising the spatiotemporal plasticity of wolf–prey relationships. The relative role of prey density and other factors (e.g., antipredator behavioural responses) should be assessed at longer temporal scales. [ABSTRACT FROM AUTHOR]

Deer hunting was heavily ritualized in medieval Europe, as indicated by historical and archaeological evidence; it also emphasized social differentiation. The butchery of a deer carcass ('unmaking') was integral to the ritual and led to different body parts being destined for individuals of differing status. Archaeologically, the practice is particularly visible in high-status sites in Britain, but documentary and archaeological sources are consistent in pinpointing its earliest occurrence in twelfth-century France. In Italy, late medieval evidence for such 'unmaking' is present but is not supported by any known historical sources. Red and fallow deer were butchered in a formalized manner, whereas the data for roe deer are unclear. Although the Normans contributed to the diffusion of the 'unmaking' practice, in France it is also found outside the core area of Norman influence. The extensive spread of the practice demonstrates the connectedness of the medieval hunting culture in Europe. [ABSTRACT FROM AUTHOR]

Understanding how the nutritional properties of food resources drive foraging choices is important for the management and conservation of wildlife populations. For moose (Alces alces), recent experimental and observational studies during the winter have shown macronutrient balancing between available protein (AP) and highly metabolizable macronutrients (total non‐structural carbohydrates [TNC] and lipids). Here, we combined the use of continuous‐recording camera collars with plant nutrient analyses and forage availability measurements to obtain a detailed insight into the food and nutritional choices of three wild moose in Norway over a 5‐day period in summer. We found that moose derived their macronutrient energy primarily from carbohydrates (74.2%), followed by protein (13.1%), and lipids (12.7%). Diets were dominated by deciduous tree browse (71%). Willows (Salix spp.) were selected for and constituted 51% of the average diet. Moose consumed 25 different food items during the study period of which 9 comprised 95% of the diet. Moose tightly regulated their intake of protein to highly metabolizable macronutrients (AP:TNC + lipids) to a ratio of 1:2.7 (0.37 ± 0.002SD). They did this by feeding on foods that most closely matched the target macronutrient ratio such as Salix spp., or by combining nutritionally imbalanced foods (complementary feeding) in a non‐random manner that minimized deviations from the intake target. The observed patterns of macronutrient balancing aligned well with the findings of winter studies. Differential feeding on nutritionally balanced downy birch (Betula pubescens) leaves versus imbalanced twigs+leaves across moose individuals indicated that macronutrient balancing may occur on as fine a scale as foraging bites on a single plant species. Utilized forages generally met the suggested requirement thresholds for the minerals calcium, phosphorus, copper, molybdenum, and magnesium but tended to be low in sodium. Our findings offer new insights into the foraging behavior of a model species in ungulate nutritional ecology and contribute to informed decision‐making in wildlife and forest management. [ABSTRACT FROM AUTHOR]

Predators impose top‐down forces on prey populations, with the strength of those effects often varying over space and time and among demographic groups. In ungulates, predation risk is typically greatest for neonatal offspring, with some suggesting that predators can key in on adult activity to locate hidden neonates. However, few field studies to date have been able to directly assess the influence of maternal care on ungulate neonate survival. Using a population of white‐tailed deer under heavy coyote predation pressure, we tested the maternal dispersion hypothesis, which suggests the dispersion of maternal activity temporally and spatially attenuates risk of predation for ungulate neonates during this vulnerable altricial phase. We compared support for this hypothesis with more commonly tested hypotheses regarding the influence of habitat conditions and intrinsic factors on neonatal survival. Fawn survival to 16 weeks was 27.7%, with coyotes accounting for 59% of fawn mortalities. In support of our maternal temporal diffusion hypothesis, we found that neonatal survival decreased as more maternal visits (proportionally) occurred at night. The only other significant (p <.1) predictor of fawn survival was birth timing, with fawn survival decreasing as the season progressed. Given that fawn survival declined as the proportion of nighttime visits increased, and that wild pig presence and human disturbance can push doe and fawn activity toward nocturnal hours, additional research is needed to determine whether managing pig and human disturbance can decrease fawn mortality. More broadly, given the importance of recruitment in ungulate population dynamics, our finding opens a potentially important new line of inquiry on how maternal behaviors influence predation risk in large animal predator–prey ecology. [ABSTRACT FROM AUTHOR]

Simple Summary: China's deer population is rich in resources and species; how to improve the health status of the deer population and reduce the occurrence of intestinal diseases is a necessary direction for current research. In this study, Enterococcus faecalis was used as the research object to explore the effect of lactic acid bacteria on deer growth performance and intestinal health and to further investigate the role played by lactic acid bacteria on the intestinal health status of deer. The results of this study showed that the addition of Enterococcus faecalis to the feed promoted weight gain, improved immune function, altered the structure of the intestinal flora of fallow deer, especially increasing the abundance of Firmicutes phylum, and increased the abundance of the Ruminococcaceae and Lacertococcaceae at the same phylum as that of Enterococcus faecalis. Furthermore, metabolomic analyses also revealed that Enterococcus faecalis supplementation increased the metabolism of lipids, carbohydrates and phospholipids by the intestinal microbiota. In conclusion, this study revealed the beneficial effects of lactic acid bacteria on growth traits, immunity and intestinal health of deer and provided a scientific basis for the further development of deer-specific probiotic products to promote intestinal health and reduce intestinal diseases in deer species in the future. Intestinal diseases are one of the diseases that affect the growth and immunity of deer. Currently, more lactic acid bacteria (LAB) are available as feed additives to improve the intestinal ecological balance of ruminants in production practices. In this study, Enterococcus faecalis was supplemented in the feed of fallow deer for 170 d, and body weights, blood indices and immune levels of fallow deer were counted at 35, 65 and 170 d. The effects of Enterococcus faecalis on the intestinal microbiota and the metabolism of fallow deer were analysed using 16S rDNA and UPLC-MS/MS methods. The results showed that the addition of Enterococcus faecalis to the diet improved body weight and immune function and increased the aggregation of gut microbiota in fallow deer. The addition of Enterococcus faecalis altered the community structure of intestinal microorganisms in fallow deer and increased the number of beneficial bacteria. In addition, combined with metabolomics analysis, it was found that supplementation with Enterococcus faecalis significantly altered the metabolites of fallow deer, mainly regulating lipid metabolism, carbohydrate metabolism and phospholipid metabolism. In conclusion, this study presents, for the first time, evidence that the LAB strain Enterococcus faecalis can be used as a potential probiotic for deer and points to a new direction for the treatment of intestinal disorders in the deer family. [ABSTRACT FROM AUTHOR]

Neolicaphrium recens (Mammalia, Litopterna) was a slender South American Native Ungulate (SANU). The assessment of its body size is a big challenge because there are no living representatives and no clear close morphological analogues. In this contribution, we report for the first time a femur of N. recens exhumed from Late Pleistocene sediments of Uruguay, improving the knowledge of the postcranial skeleton of the species. This stylopodial element is important for doing palaeobiological inferences, including estimating the body mass. We performed body mass estimations by previous allometric equations. As a method to directly test the models and their usefulness in fossils, we applied the equations on six specimens of extant deers of known body weight and with skeletons preserved. According to the obtained data, the transverse width at the middle of the diaphysis and the circumference of the bone at that level can be used with confidence to estimate the body mass of fossil ungulates. We point out a set of more probable values of body mass for N. recens: 28.76298 ± 10.6412, 27.78496 ± 8.0576, and 26.72 ± 10.42 kg. Morphological variables as well as the models for estimating body masses in fossils are widely discussed. [ABSTRACT FROM AUTHOR]

The structure of cellular prion proteins encoded by the prion protein gene (PRNP) impacts susceptibility to transmissible spongiform encephalopathies, including chronic wasting disease (CWD) in deer. The recent emergence of CWD in Northern European reindeer (Rangifer tarandus), moose (Alces alces alces) and red deer (Cervus elaphus), in parallel with the outbreak in North America, gives reason to investigate PRNP variation in European deer, to implement risk assessments and adjust CWD management for deer populations under threat. We here report PRNP-sequence data from 911 samples of German red, roe (Capreolus capreolus), sika (Cervus nippon) and fallow deer (Dama dama) as well as additional data from 26 Danish red deer close to the German border and four zoo species not native to Germany. No PRNP sequence variation was observed in roe and fallow deer, as previously described for populations across Europe. In contrast, a broad PRNP variation was detected in red deer, with non-synonymous polymorphisms at codons 98, 226 and 247 as well as synonymous mutations at codons 21, 78, 136 and 185. Moreover, a novel 24 bp deletion within the octapeptide repeat was detected. In summary, 14 genotypes were seen in red deer with significant differences in their geographical distribution and frequencies, including geographical clustering of certain genotypes, suggesting "PRNP-linages" in this species. Based on data from North American CWD and the genotyping results of the European CWD cases, we would predict that large proportions of wild cervids in Europe might be susceptible to CWD once introduced to naive populations. [ABSTRACT FROM AUTHOR]

Populations of large herbivores can be efficiently managed by regulating the number of females, which is directly related to recruitment. This study aims to determine seasonal changes in the attractiveness of salt water to female Sika deer (Cervus nippon) in order to cull females more efficiently. Females drank saltwater well at similar times of the year for saltwater with different installation dates. In particular, the frequency of drinking increased from early April to mid-July. Previous studies have shown that salt water is an effective attractant for capturing females, but the results of this study suggest that females may be captured more effectively in spring and early summer. This is the birthing season for sika deer. Culling females in late pregnancy and lactation may also directly reduce recruitment due to higher mortality of young. Saltwater-induced culling of female sika deer may be an effective method of managing overpopulation of the deer. [ABSTRACT FROM AUTHOR]

Chronic wasting disease (CWD) is now a major health concern among US cervid populations, including white-tailed deer (Odocoileus virginianus). Texas is home to the greatest number of deer breeding facilities and most bred deer in the United States. The vast majority of the state's CWD cases occur at deer breeding facilities. CWD risk on deer breeding facilities is largely owed to the close proximity of deer pens. To reduce the risk of CWD transmission, state authorities use strict management strategies including culling of infected and potentially infected deer populations. State wildlife biologists provide recommendations that inform the CWD containment strategies. However, there is contention between deer breeders and state wildlife authorities over CWD. Property contradictions—whereby the state must manage the public trust deer herd on privately owned land—complicates the state's biopower and provides breeders the opportunity to reconfigure biopolitics. We draw on qualitative research data to demonstrate how CWD science becomes entangled among trophy antler genetics, physical abnormalities, ranching science, conspiracy narratives, hunting culture, and public trust property. We show how both the state and private entrepreneurs manipulate scientific knowledge toward the regulation of bodies: hunters, ranchers, and deer breeders on one hand, and the animals themselves, on the other. [ABSTRACT FROM AUTHOR]

Musk is an important animal product, but the musk secretion mechanism of forest musk deer (Moschus berezovskii) is still unclear. The musk synthesis process in forest musk deer is extremely complex, and many raw materials are directly or indirectly derived from forest musk deer blood. In this study, metabolomics was used to analyze the blood of forest musk deer in secretory and non-secretory phases for the first time, aim at explaining the secretion mechanism from the perspective of blood metabolism. We found that P450-related, choline-related, axonal regeneration and other pathways and related metabolites were significantly enriched during the musk secretion of forest musk deer. These pathways and metabolites related to P450 and choline in blood may have important implications for the mechanism of musk secretion in forest musk deer, because blood components were closely related to musk components and could provide raw materials for musk synthesis in musk gland cells. [ABSTRACT FROM AUTHOR]

Increasing consumer interest in game meat has globally expanded wild animal farming, with deer being a prominent non-traditional species farmed in numerous countries. Consequently, methods for assessing the welfare of captive animals have been developed over the last few decades, and despite its limitations, the Five Domains Model has become one of the most popular practical tools for welfare assessment. Our review focuses on welfare indicators in farmed red and fallow deer, the primary deer species in European farms. Among 57 studies, the majority emphasized deer health and nutrition, neglecting aspects like behavior and mental state. Notably, a significant portion explored human-animal interactions, especially in deer transportation. Summarizing literature for each domain, we propose reliable physical, behavioral, and physiological welfare indicators for farmed red and fallow deer. This comprehensive approach addresses current research gaps and contributes to enhancing the overall well-being of farmed deer. [ABSTRACT FROM AUTHOR]

BACKGROUND RESULTS CONCLUSION Deer‐derived materials (antler, venison, fetus, penis, bone, tail, and others) are some of the most valuable traditional animal‐based medicinal and food materials in China. In production, processing, and trade, the quality of deer products varies. The market is confusing, and counterfeit and shoddy products are common. There is an urgent need to establish an accurate identification method.Two pairs of primers suitable for identifying deer‐derived medicinal materials were obtained by screening the cytochrome oxidase I (COI) sequences of 18 species from nine genera of the deer family. The two primers were used to identify the species and adulteration of 22 batches of commercially available deer‐derived products with a mini‐barcode combining high‐resolution melting (HRM) technology and methodical investigation. Deer‐derived materials (sika and red deer) were correctly identified by species using varying DNA amounts (1 to 500 ng). The two pairs of primers COI‐1FR and COI‐2FR yielded melting temperatures (Tm) of 80.55 to 81.00 °C and 82.00 to 82.50 °C for sika deer, and 81.00 to 82.00 °C and 81.40 to 82.00 °C for red deer. Twenty‐two batches of commercially available samples were analyzed by HRM analysis and conventional amplification sequencing, and it was found that the species samples had an error rate of species labeling of 31.8%. Four batches of samples were identified as mixed (adulterated) in the HRM analysis.The combination of DNA mini‐barcode with HRM analysis facilitated the accurate identification of species of deer‐derived materials, especially the identification of samples in an adulterated mixed state. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Populations in isolated and small fragments lose genetic variability very fast and are usually of conservation concern because they are at greater risk of local extinction. The largest native deer in South America, Blastocerus dichotomus (Illiger, 1815), is a Vulnerable species according to the IUCN categorization, which inhabits tropical and subtropical swampy areas. In Argentina, its presence has been restricted to four isolated fragments. Here we examine the genetic diversity and differentiation among three of them, including the three different patches that form the southernmost population, using 18 microsatellite markers genotyped by Amplicon Sequencing of DNA extracted from fecal samples. Genetic diversity was low (HE < 0.45) in all three populations studied. We found three genetic clusters compatible with the geographic location of the samples. We also found a metapopulation dynamics that involves the patches that make up the southernmost population, with evidence of a barrier to gene flow between two of them. Our results point to the creation of a corridor as a necessary and urgent management action. This is the first study, at the population level, employing microsatellite genotyping by Amplicon Sequencing with non-invasive samples in an endangered species. [ABSTRACT FROM AUTHOR]

Resource pulses are ecologically important phenomenon that occur in most ecosystems globally. Following optimal foraging theory, many consumers switch to pulsatile foods when available, examples of which include fruit mast and vulnerable young prey. Yet how the availability of resource pulses shapes the ecology of predators is still an emerging area of research; and how much individual variation there is in response to pulses is not well understood. We hypothesized that resource pulses would lead to dietary convergence in our population, which we tested by tracking both population‐level and individual coyote diets for 3 years in South Carolina, USA. We (1) described seasonal dietary shifts in relation to resource pulses; (2) compared male and female diets across seasons; and (3) tested this dietary convergence hypothesis by quantifying individual dietary variation both across and within periods when resource pulses were available. We found that pulses of white‐tailed deer fawns and blackberries composed over half of coyote diet in summer, and persimmon fruits were an important component in fall. Male and female coyotes generally had similar diets, but males consumed more deer in fall, perhaps driven by scavenging more. We found support for our dietary convergence hypothesis, where individuals had more similar diets during resource pulses compared to a non‐pulse period. We also found that this convergence happened before peak availability, suggesting a non‐symmetric response to pulse availability. We show that nearly all coyotes eat fawns, suggesting that targeted efforts to remove "fawn killers" would be in vain. Instead, given how quickly coyotes collectively converge on resource pulses, our findings show that resource pulses could potentially be used by managers to alter the behavior of apex predators. More broadly, we open a new line of inquiry into how variation in individual foraging decisions scales up to shape the effects of resource pulses on ecological communities. [ABSTRACT FROM AUTHOR]

The Barda Wildlife Sanctuary (Barda WLS) is a semi-arid ecosystem that plays an important role in the conservation of mammalian carnivores. It is essential to evaluate the population of important wild prey species in Barda, which has conservation implications for mammalian predators and themselves. We assessed the density, biomass, and population structure of wild prey species by surveying road (n = 10) and trail (n = 8) transects using the distance sampling framework method. We used male-to-female sex ratios and the percent contribution of each age class to assess population structure. Mean group size (MGS), median group size (Mdgs), and mean crowding (Mc) were calculated to analyze group size. Of the species studied, Indian peafowl had the highest density (51.61 ± 7.08 individuals/km2), followed by wild pig (10.77 ± 4.59), blue bull (3.50 ± 0.51), Indian hare (3.03 ± 0.73), and spotted deer (0.62 ± 0.04). The male-to-adult female sex ratio was biased towards females, except for Indian peafowl. All species had small group sizes except wild pigs. The results of this study can aid in its development as one of the important protected areas in Gujarat for the conservation of mammalian predators, along with framing conservation strategies for Barda WLS. [ABSTRACT FROM AUTHOR]