Search

Antigen-presenting cells (APCs) play an important role in virus infection control by bridging innate and adaptive immune responses. Macrophages and dendritic cells (DCs) possess various surface receptors to recognize/internalize antigens, and antibody binding can enhance pathogen-opsonizing uptake by these APCs via interaction of antibody fragment crystallizable (Fc) domains with Fc receptors, evoking profound pathogen control in certain settings. Here, we examined phagocytosis-enhancing potential of Fc domains directly oriented on a retroviral virion/virus-like particle (VLP) surface. We generated an expression vector coding a murine Fc fragment fused to the transmembrane region (TM) of a retroviral envelope protein, deriving expression of the Fc-TM fusion protein on the transfected cell surface and production of virions incorporating the chimeric Fc upon co-transfection. Incubation of Fc-displaying simian immunodeficiency virus (SIV) with murine J774 macrophages and bone marrow-derived DCs derived Fc receptor-dependent enhanced uptake, being visualized by imaging cytometry. Alternative preparation of a murine leukemia virus (MLV) backbone-based Fc-displaying VLP loading an influenza virus hemagglutinin (HA) antigen resulted in enhanced HA internalization by macrophages, stating antigen compatibility of the design. Results show that the Fc-TM fusion molecule can be displayed on certain viruses/VLPs and may be utilized as a molecular adjuvant to facilitate APC antigen uptake. • Antibody Fc can be surface-displayed on virions and virus-like particles (VLPs). • Fc-displaying viruses/VLPs undergo enhanced uptake by antigen-presenting cells. • Imaging cytometry depicts enhanced Fc-displayed virus internalization. • Loading antigen-compatible design can harness immunization regimens. [ABSTRACT FROM AUTHOR]

Smart renewable energy harvesting has been implemented from hybrid sources such as solar and wind. The wireless sensor node is created for monitoring surface water. In the intelligent building, electrical energy is harvested from the hybrid source of solar and wind energy. The source energy was selected for the harvesting process by using a fuzzy controller. In this proposed method, piezo-electric reverse electro-wetting on di-electric energy harvesting is proposed where constant DC voltage is generated by a rectifier. A DC-DC converter is designed to power up the remote read-out sensor. The produced charge is transformed by a charge amplifier with the proportion of output voltage that is delivered to the wireless receiver. The harvested DC voltage varies with the temperature and external environmental effect. In our work, we obtained 6 × 10−3 W/m² of voltage and this harvested energy is monitored using the Internet of Things (IoT) by the proposed EHOR (Energy Harvested Optimized Routing) algorithm. [ABSTRACT FROM AUTHOR]

The intricate immunomodulatory mechanism underlying the onset and progression of chronic rhinosinusitis with nasal polyps (CRSwNP) in chronic sinusitis with nasal polyps remains poorly understood, particularly regarding the regulatory role of dendritic cells (DCs). MicroRNA has been identified as a modulator of DC function. Primary cells have a short time in vitro and their biological characteristics have not changed greatly which can better reflect the growth state of cells in the body, so as to obtain data closer to the physiological function in the body. Our research group has been engaged in the culture of human nasal epithelial progenitor cells (hNEPCs) for a long time, and in previous investigations, we observed downregulation of microRNA‐124 (miR‐124) in patients with chronic sinusitis and nasal polyps. Thus, our current study aims to elucidate the regulatory mechanism of DCs in chronic sinusitis with polyps by examining the impact of miR‐124 on DCs in this context. To accomplish this, we took the nasal mucosa of CRSwNP patients and normal nasal mucosa of non‐rhinitis patients for primary culture. We isolated exosomes from these cells for co‐culture experiments with DCs. Flow cytometry analysis was employed to assess the maturation phenotypes (MHC II, CD80 and CD86), cell cycle and apoptosis levels of DCs. Furthermore, enzyme‐linked immunosorbent assay was utilized to measure the levels of pro‐inflammatory cytokines (IL‐10, IL‐12 and IL‐23). The TLR4/NF‐κB signalling pathway in co‐cultured DCs was evaluated using the western blot assay. Through querying the Targetscan website, we predicted Rab27a as a target gene of miR‐124, which was subsequently validated through double luciferase assay, flow cytometry and other experimental approaches. Our findings demonstrate that MiR‐124 modulates the secretion of CRSwNP epithelial exosomes, thereby influencing DC function via its interaction with the target gene Rab27a. These results offer novel insights into potential therapeutic targets for the treatment of CRSwNP. [ABSTRACT FROM AUTHOR]

Background: Diabetic cataract (DC) is a common complication of diabetes and its etiology and progression are multi-factorial. In this study, the roles of specific protein 1 (SP1) and fibroblast growth factor 7 (FGF7) in DC development were explored. Methods: DC cell model was established by treating SRA01/04 cells with high glucose (HG). MTT assay was conducted to evaluate cell viability. Transwell assay and wound-healing assay were performed to assess cell migration and invasion. Western blot assay and qRT-PCR assay were conducted to measure the expression of N-cadherin, E-cadherin, Collagen I, Fibronectin, SP1 and FGF7 expression. CHIP assay and dual-luciferase reporter assay were conducted to analyze the combination between FGF7 and SP1. Results: FGF7 was upregulated in DC patients and HG-induced SRA01/04 cells. HG treatment promoted SRA01/04 cell viability, migration, invasion and epithelial-mesenchymal transition (EMT), while FGF7 knockdown abated the effects. Transcription factor SP1 activated the transcription level of FGF7 and SP1 overexpression aggravated HG-induced SRA01/04 cell injury. SP1 silencing repressed HG-induced SRA01/04 cell viability, migration, invasion and EMT, but these effects were ameliorated by upregulating FGF7. Additionally, SP1 knockdown inhibited the PI3K/AKT pathway by regulating the transcription level of FGF7. Conclusion: Transcription factor SP1 activated the transcription level of FGF7 and the PI3K/AKT pathway to regulate HG-induced SRA01/04 cell viability, migration, invasion and EMT. [ABSTRACT FROM AUTHOR]

A web search, an online video, a connected Nest device, and hundreds of cloud services all give us a response in a fraction of a second. But what really happens when we click search or send a request. The request travels over the public internet and into fiber network. Millions of requests or packets of data travel through miles of cable over land and under sea, converging at one of the many data centers that operate all over the world. Data Center (DC) is the core site of data operation, storage and forwarding, which is also an important part of cloud platform. A large number of commercial switches and servers are usually deployed at the DC. The DC is a complex set of facilities. Data center networks (DCN) is a network applied in the DC, because the traffic in DC presents the typical characteristics of centralized exchange data and increased traffic, which puts forward further requirements for the DCN. DCN connects a large-scale server cluster and is a bridge for data transmission and storage. With the expansion of DC and the increasing number of service types, communication within data centers becomes more frequent. On the other hand, traffic between data centers has also increased dramatically. Considering the multi-layered transmission mode and traffic characteristics of DCN, this paper proposes a software defined networking (SDN) -based multi-layered traffic scheduling scheme for DCN, which is mainly focused on hop count, criticality and cost. Moreover, based on SDN architecture and Deep Q-Network of Reinforcement Learning (RL), the intelligent multi-layered traffic scheduling scheme is proposed to obtain the current optimal global routing strategy according to the real-time traffic demand in the network. The simulation results show that the proposed scheme outperforms benchmarks in terms of average throughput, normalized total throughput, link bandwidth utilization, average round-trip time and network traffic bandwidth loss rate. [ABSTRACT FROM AUTHOR]

Researchers have found that gentrification is less likely in Black neighborhoods than in White or Latinx neighborhoods, and that gentrification looks different in Black neighborhoods. For example, researchers have found that Black neighborhoods experience marginal gentrification—changes in the educational level but not the income of residents. This study uses Census and National Historical Geographic Information System (NHGIS) data to explore the relationship between gentrification and racial change in Washington, DC between 2000 and 2019. We measure gentrification using four distinct but related measures: change in home value, rent, average educational level, and household income. The results show a positive association between changes in the percentage of residents with college degrees and the percentage of White residents in neighborhoods that were majority Black in 2000. We also find a positive association between changes in the percentage of Latinx residents and the average rent. We do not find a significant relationship between racial change and changes in home value and average income. Our findings point to the importance of including race in models of gentrification as well as using different measures of gentrification to capture it more fully.

Smart renewable energy harvesting has been implemented from hybrid sources such as solar and wind. The wireless sensor node is created for monitoring surface water. In the intelligent building, electrical energy is harvested from the hybrid source of solar and wind energy. The source energy was selected for the harvesting process by using a fuzzy controller. In this proposed method, piezo-electric reverse electro-wetting on di-electric energy harvesting is proposed where constant DC voltage is generated by a rectifier. A DC-DC converter is designed to power up the remote read-out sensor. The produced charge is transformed by a charge amplifier with the proportion of output voltage that is delivered to the wireless receiver. The harvested DC voltage varies with the temperature and external environmental effect. In our work, we obtained 6 × 10−3 W/m2 of voltage and this harvested energy is monitored using the Internet of Things (IoT) by the proposed EHOR (Energy Harvested Optimized Routing) algorithm.

The equitable distribution of docked bikeshare is an important issue for transportation decision-makers. Gini coefficients and Lorenz curves can be used to study transportation inequality. This study used these methods to achieve three outcomes. First, this study identified a gap in bikeshare equity literature and provided a more nuanced measure of bikeshare supply to better evaluate how well bikeshare meets demand. Second, the updated measure of distributional equity was used to evaluate bikeshare equity in Washington, DC. Third, using historical trip data, the Gini coefficient calculations indicated which trip types are better served. Comparison of the results highlighted a potential conflict in program goals: equity versus supporting existing bikeshare members. Policy feedback may bias this trade-off in favor of members, reinforcing spatial inequalities. Overall, this paper further demonstrated how Gini coefficients and Lorenz curves can be useful tools for evaluating inequality in transportation systems. [ABSTRACT FROM AUTHOR]

Esophageal squamous cell carcinoma (ESCC) is characterized by molecular heterogeneity with various immune cell infiltration patterns, which have been associated with therapeutic sensitivity and resistance. In particular, dendritic cells (DCs) are recently discovered to be associated with prognosis and survival in cancer. However, how DCs differ among ESCC patients has not been fully comprehended. Recently, the advance of single-cell RNA sequencing (scRNA-seq) enables us to profile the cell types, states, and lineages in the heterogeneous ESCC tissues. Here, we dissect the ESCC tumor microenvironment at high resolution by integrating 192,078 single cells from 60 patients, including 4379 DCs. We then used Scissor, a method that identifies cell subpopulations from single-cell data that are associated bulk samples with genomic and clinical information, to stratify DCs into Scissorhi and Scissorlow subtypes. We applied the Scissorhi gene signature to stratify ESCC scRNAseq patient, and we found that PD-L1, TIGIT, PVR and IL6 ligand-receptor-mediated cell interactions existed mainly in Scissorhi patients. Finally, based on the Scissor results, we successfully developed a validated prognostic risk model for ESCC and further validated the reliability of the risk prediction model by recruiting 40 ESCC clinical patients. This information highlights the importance of these genes in assessing patient prognosis and may help in the development of targeted or personalized therapies for ESCC. [ABSTRACT FROM AUTHOR]

Dendritic cell (DC)-based vaccines have emerged as a promising strategy in cancer immunotherapy due to low toxicity. However, the therapeutic efficacy of DC as a monotherapy is insufficient due to highly immunosuppressive tumor environment. To address these limitations of DC as immunotherapeutic agent, we have developed a polymeric nanocomplex incorporating (1) oncolytic adenovirus (oAd) co-expressing interleukin (IL)-12 and granulocyte-macrophage colonystimulating factor (GM-CSF) and (2) arginine-grafted bioreducible polymer with PEGylated paclitaxel (APP) to restore antitumor immune surveillance function in tumor milieu and potentiate immunostimulatory attributes of DC vaccine. Nanohybrid complex (oAd/APP) in combination with DC (oAd/APP+DC) induced superior expression level of antitumor cytokines (IL-12, GM-CSF, and interferon gamma) than either oAd/APP or DC monotherapy in tumor tissues, thus resulting in superior intratumoral infiltration of both endogenous and exogenous DCs. Furthermore, oAd/APP+DC treatment led superior migration of DC to secondary lymphoid organs, such as draining lymph nodes and spleen, in comparison with either monotherapy. Superior migration profile of DCs in oAd/APP+DC treatment group resulted in more prolific activation of tumor-specific T cells in these lymphoid organs and greater intratumoral infiltration of T cells. Additionally, oAd/ APP+DC treatment led to lower subset of tumor infiltrating lymphocytes and splenocytes being immunosuppressive regulatory T cells than any other treatment groups. Collectively, oAd/APP+DC led to superior induction of antitumor immune response and amelioration of immunosuppressive tumor microenvironment to elicit potent tumor growth inhibition than either monotherapy. [ABSTRACT FROM AUTHOR]

Objective: To evaluate a PCR based method of Polyacrylamide gel electrophoresis of short tandem repeats and its quantification for detecting donor chimerism after haematopoietic stem cell transplantation in acute leukaemias. Methods: The descriptive study was conducted at Genetic Resource Centre (GRC) Lab Rawalpindi from Feb 2018 - Nov 2020. A total of twenty patients with acute leukaemias having undergone HSCT were selected and assessed for the analysis of chimerism status. DNA extraction from the whole blood was done by chelex method and short tandem repeats were amplified by using conventional STR-PCR assay. Electrophoresis was carried out and 6% Polyacrylamide gels were used for the resultant amplified DNA products and then followed by their densitometry. These patients had undergone HSCT from Pakistan Institute of Medical Science and Armed Forces Bone Marrow Transplant Centre. Results: The peaks in the PAGE densitometry represented the donor chimerism in all post transplant samples of the patients. Conclusion: Our study showed that densitometry of STR PCR PAGE is a useful and cheaper method for demonstration of donor chimerism in acute leukaemia patients having undergone HSCT. Hence this method can be a valuable option in the monitoring of chimerism status in these patients and therefore helps in preventing graft failure by fast and early treatment strategies for these patients. [ABSTRACT FROM AUTHOR]

Multiracial neighborhood integration has become more common in U.S. metropolitan areas over the past three decades. This article takes up the question: are residents satisfied living in multiracial neighborhoods? Traditional theories of racial change predict low levels of satisfaction in these neighborhoods, while newer studies question that prediction. The article uses data representing all residents of multiracial neighborhoods in the Washington, DC, area to study neighborhood satisfaction in multiracial neighborhoods. The analysis finds evidence of shared satisfaction among residents regardless of race: large and equal shares of each racial group were satisfied. White residents were less satisfied than white residents of neighborhoods elsewhere in the metropolitan region, but were unlikely to perceive neighborhood decline. The shared satisfaction among residents of all races and the lack of racial antipathy to change among white residents suggests that multiracial neighborhoods offer sites to promote racial equity. [ABSTRACT FROM AUTHOR]

This article focuses on the archive of the Washington Female Orphan Asylum, founded in 1815, and places the study of philanthropy in conversation with scholarship on the archive in histories of slavery, colonization, and trauma. It argues, first, that philanthropic and reform institutions such as the asylum were domestic sites of empire and that their archives reveal the reach of statecraft into the intimate lives of women and families. The article explores, second, the role of emotion in archival research, which can highlight an archive's construction and its silences. The relinquishments within the asylum's records provoke emotion; as fragmentary evidence, they testify to trauma and demand the historian's care. [ABSTRACT FROM AUTHOR]

With due span of time, Cloud computing has grown phenomenally as it is a best approach for using the scalable shared pool of resources at economical cost. Though it seems easy but distribution of the jobs assigned to various Virtual Machines (VMs) is a cumbersome task for the Data Centre (DC) Controller. Various techniques have been proposed from time-to-time for balancing the load on distinct VMs available in the DCs. The primarily used load balancing techniques are Round Robin, Equal Spread Current Execution and Throttled. The major thrust for using the aforementioned techniques is to improve the performance parameters such as Overall Response Time (ORT), Data Center Processing Time (DCPT) and Cost. In this manuscript, a new algorithm named Pragmatic Load Balancing (PLB) has been designed and developed for optimization of afore-said parameters and various simulation scenarios have also been created for the evaluation of these parameters. In the given Scenarios created by using Cloud Analyst Simulator, different number of VMs like 20, 40, 60, 80 and 100 have been allotted with distinct number of DCs from 1 to 5 to the application at each Cloud Configuration and the divulged that the value of the performance parameters have been improved by using the PLB Algorithm in comparison to other available Load Balancing Algorithms. [ABSTRACT FROM AUTHOR]

In this study, the DC and RF performances of multi fingered AlGaN/GaN are studied in simulation, and the DC and RF performance of the traditional T-gate HEMT and the multi-finger HEMT are compared.The reason for the performance difference is that the use of multi finger gate technology reduces the total parasitic capacitance and the total transit time of the carrier through the device compared to conventional T-gate devices, resulting in an increase in FT and Fmax. Based on the study of conventional AlGaN/GaN HEMT devices, an InAlN/GaN HEMT device with two-finger gate is designed. Due to the matching of InAlN and GaN lattice, the polarisation effect and carrier density of the heterojunction formed by InAlN and GaN are larger than those of conventional AlGaN/GaN heterojunction, which can ensure the device to have a larger output current while operating at high frequency. The results show that the peak FT and Fmax of the proposed InAlN/GaN HEMT are 239.5 GHz and 331.5 GHz, respectively. [ABSTRACT FROM AUTHOR]

Multifunctional building materials with self-sensing capability have great potential for civil engineering applications. The self-sensing capability of typically calcium aluminosilicate matrices of cementitious or geopolymer materials is adopted by admixing electrically conductive admixtures in an amount that ensures optimal electrical properties and their proportionality to mechanical loading. The paper aims to evaluate the self-sensing capability of 4 metashale geopolymer mortars with graphite powder (GP) and carbon fibers (CF) in different ratios, including MGF 5/0, MGF 4.5/0.5, MGF 4/1, and MGF 3/0. The 4-probe measurements at 21 V DC input voltage on (100 × 100 × 100) mm3 samples with embedded copper-grid electrodes evaluate the gauge factor, which corresponds to the monitored changes in electrical resistivity. Despite the limitations of DC measurements, the self-sensing capability is observed for all the mixtures. The most promising response to dynamic loading with an FCR of 0.018%, is observed for the MGF 4.5/0.5 sample.

BackgroundVasovagal syncope (VVS) is a prevalent medical condition with a lack of efficient methods for its detection.AimThis study aimed to explore an objective clinical indicator in diagnosing VVS.MethodsThe retrospective analysis involved clinical data of 243 syncope patients from 1 June 2020 to 31 July 2023. Among them, 108 patients had a negative result in the tilt test (TTT), while the remaining 135 patients had a positive result in the TTT. Relevant statistical methods were utilized to examine the correlation between VVS and different indicators of heart rate variability.ResultsAfter screening, 354 patients being considered for VVS were evaluated, resulting in a final sample size of 243. Sex, age, deceleration capacity (DC), and standard deviation of all normal-to-normal intervals (SDNNs) were the variables that showed statistical significance between the TTT(−) group and the TTT(+) group. Independent risk factors identified by multivariate logistic regression were DC [odds ratio (OR) 1.710, 95% confidence interval (CI) 1.388–2.106, P

Abstract The expansion of the DC fast‐charging (DCFC) network is expected to accelerate the transition to sustainable transportation by offering drivers additional charging options for longer journeys. However, DCFC places significant stress on the grid, leading to costly system upgrades and high monthly operational expenses. Incorporating energy storage into DCFC stations can mitigate these challenges. This article conducts a comprehensive review of DCFC station design, optimal sizing, location optimization based on charging/driver behaviour, electric vehicle charging time, cost of charging, and the impact of DC power on fast‐charging stations. The review is closely aligned with current state‐of‐the‐art technologies and encompasses academic research contributions. A critical assessment of 146 research articles published from 2000 to 2023 identifies research gaps and explores avenues for future study based on the literature review.

Summary: Background: Monitoring hepatitis B virus (HBV) and hepatitis C virus (HCV) liver-related morbidity and mortality is key to evaluate progress towards elimination targets. Methods: HBV and HCV notifications in NSW, Australia (1995–2022) were linked to hospital and mortality records. Temporal trends in decompensated cirrhosis (DC), hepatocellular carcinoma (HCC), and mortality were evaluated among people notified for HBV and HCV. Segmented Poisson regression models were used to assess the impact of the viral hepatitis elimination era (1 January 2015–31 December 2022) on advanced liver disease and mortality. Findings: During 1995–2022, there were 64,865 people with an HBV notification and 112,277 people with an HCV notification in NSW. Between 2002 and 2022, there were significant reductions in age-adjusted HBV- and HCV-related DC, HCC, and liver-related mortality. Among those with HBV, age-standardised incidence per 1000 person-years (py) in 2002, 2015, and 2022 was 3.08, 1.47, and 1.16 for DC (p

The High-Efficiency Video Coding (HEVC/H.265) standard was created by a group called the Joint Collaborative Team on Video Coding (JCT-VC) and was released in 2013.In HEVC Rate-Distortion Optimization (RDO) is employed for partitioning the coding tree units into coding units recursively due to this the computational complexity increases. However, changing from macroblocks to coding tree units the compression efficiency increases and increases the encoding of the video sequence. This paper presents a Support Vector Machine (SVM)-based method for finding the fastest coding unit division in intra-prediction HEVC without compromising compression efficiency. All partitions of CTU are assessed using five characteristics: Root Mean Square Error (RMSE), Sub CU Complexity Difference (SCCD), Standard Deviation (SD), Directional Complexity (DC), and Quantization Parameter (QP) to optimize the intra-prediction of HEVC in all intra-configurations. The simulation results reveal combining directional complexity and standard deviation yields a more accurate classification. SVM was used to separate split-unsplit data, and the conventional rate-distortion optimisation technique was applied to separate samples that were difficult to separate. The results show that the encoding process has been completed 67.44% faster, albeit with a marginal increase in bit rate. [ABSTRACT FROM AUTHOR]

A heavy-duty engine is homologated in a test cell. However, starting with Euro VI regulation, the in-service conformity is controlled with the engine installed in the vehicle using portable emission measurement systems (PEMS). In Europe, the application of solid particle number (SPN) PEMS started in 2021 for compression ignition (diesel) vehicles and in 2023 for positive ignition vehicles, thus including those operating with compressed natural gas (CNG). Even though today only particles with sizes > 23 nm are regulated, the Euro 7 proposal includes particles > 10 nm. There are not many studies on the accuracy of the SPN PEMS, especially for heavy-duty applications. In this study, PEMS measuring > 23 and >10 nm from two instrument manufacturers were compared with laboratory-grade instruments. The particle detector of one PEMS was a condensation particle counter (CPC), and of the other a the diffusion charger (DC). The results showed the robustness and good accuracy (40% or 1 × 1011 #/kWh) of the PEMS for ambient temperatures from −7 °C to 35 °C, active regeneration events, different fuels (Diesel B7, HVO, and CNG), different test cycles, cold start or hot engine operations, and high exhaust gas humidity content. Nevertheless, for the DC-based PEMS, sensitivity to pre-charged urea particles was identified, and for the CPC-based PEMS, sensitivity to pressure changes with one vehicle was nnoticed. Nevertheless, the results of this study confirm that the PEMS are accurate enough to measure even the stricter Euro 7 limits. [ABSTRACT FROM AUTHOR]

Type I interferons (IFN-I) play pivotal roles in tumor therapy for three decades, underscoring the critical importance of maintaining the integrity of the IFN-1 signaling pathway in radiotherapy, chemotherapy, targeted therapy, and immunotherapy. However, the specific mechanism by which IFN-I contributes to these therapies, particularly in terms of activating dendritic cells (DCs), remains unclear. Based on recent studies, aberrant DNA in the cytoplasm activates the cyclic GMP-AMP synthase (cGAS)- stimulator of interferon genes (STING) signaling pathway, which in turn produces IFN-I, which is essential for antiviral and anticancer immunity. Notably, STING can also enhance anticancer immunity by promoting autophagy, inflammation, and glycolysis in an IFN-I-independent manner. These research advancements contribute to our comprehension of the distinctions between IFN-I drugs and STING agonists in the context of oncology therapy and shed light on the challenges involved in developing STING agonist drugs. Thus, we aimed to summarize the novel mechanisms underlying cGAS-STING-IFN-I signal activation in DC-mediated antigen presentation and its role in the cancer immune cycle in this review. [ABSTRACT FROM AUTHOR]

Dendritic cell (DC)-based vaccines have emerged as a promising strategy in cancer immunotherapy due to low toxicity. However, the therapeutic efficacy of DC as a monotherapy is insufficient due to highly immunosuppressive tumor environment. To address these limitations of DC as immunotherapeutic agent, we have developed a polymeric nanocomplex incorporating (1) oncolytic adenovirus (oAd) co-expressing interleukin (IL)-12 and granulocyte-macrophage colony-stimulating factor (GM-CSF) and (2) arginine-grafted bioreducible polymer with PEGylated paclitaxel (APP) to restore antitumor immune surveillance function in tumor milieu and potentiate immunostimulatory attributes of DC vaccine. Nanohybrid complex (oAd/APP) in combination with DC (oAd/APP+DC) induced superior expression level of antitumor cytokines (IL-12, GM-CSF, and interferon gamma) than either oAd/APP or DC monotherapy in tumor tissues, thus resulting in superior intratumoral infiltration of both endogenous and exogenous DCs. Furthermore, oAd/APP+DC treatment led superior migration of DC to secondary lymphoid organs, such as draining lymph nodes and spleen, in comparison with either monotherapy. Superior migration profile of DCs in oAd/APP+DC treatment group resulted in more prolific activation of tumor-specific T cells in these lymphoid organs and greater intratumoral infiltration of T cells. Additionally, oAd/APP+DC treatment led to lower subset of tumor infiltrating lymphocytes and splenocytes being immunosuppressive regulatory T cells than any other treatment groups. Collectively, oAd/APP+DC led to superior induction of antitumor immune response and amelioration of immunosuppressive tumor microenvironment to elicit potent tumor growth inhibition than either monotherapy.

A novel electrical setup for a domestic house is suggested that uses both an AC bus bar fuelled by conventional electrical utilities and a DC bus bar powered by roof-mounted solar panels. Residential rooftop solar panels (RTSP) have become more and more common in recent years. Despite the fact that RTSP produce DC power, the power electronics device is set up to convert DC to AC in order to supply power to the house. As replacements for conventional AC appliances, DC appliances and Brushless DC (BLDC) motor-based devices are now readily accessible. A normal two-bedroom condominium was used for the research, and the AC bus arrangement was changed to include DC bus power from rooftop solar cells and AC bus bar power from the state power system. As before, the AC bus will continue to power high power appliances like geysers and pump engines while the DC bus will continue to power the DC appliances. A combination DC-AC house with rooftop solar electricity has been studied for feasibility using simulation studies using Matlab and Simulink. The early findings made public are encouraging for a novel combination DC-AC house setup.

The free‐standing triboelectric nanogenerator (F‐S TENG) is demonstrated to be an efficient energy conversion device. However, limited by the inherent features of high‐voltage, low alternating current and high output impedance, TENGs suffer from inefficient energy utilization and difficulties in practical applications. Therefore, the efficient harvest of mechanical energy and optimization of output are currently critical issues for TENGs. Here, a strategy is proposed for optimizing the inherent features by coupling F‐S TENG to a capacitor auto‐conversion circuit (CAC). For the first time, a specific idea is proposed explicitly to reduce the output impedance by the management circuit. Eventually, a high current density of 32 A m−2 and a charge density of 1.75 mC m−2 are achieved, which is 8.78 times higher than previous works. The power density and energy density reach to 124 W m−2 and 252 mJ m−2 under 470 and 150 Ω respectively. Then, 5000 LEDs and two lamps rated at 220 V and 50 W are manually illuminated. The resulting pulse output is utilized as a trigger source for the thyristor, enabling DC contactless switching. This work provides a novel approach to realize high‐performance DC output of TENGs and effectively reduce external load impedance. [ABSTRACT FROM AUTHOR]

Borderline personality disorder (BPD) is a severe psychiatric disorder characterized by emotion dysregulation, impulsivity, and interpersonal disturbances. Several structural and functional neuroimaging abnormalities have been described in BPD. In particular, resting-state functional magnetic resonance imaging (rs-fMRI) studies have recently suggested various connectivity alterations within and between large-scale brain networks in BPD. This review aimed at providing an updated summary of the evidence reported by the available rs-fMRI studies in BPD individuals. A search on PubMed, Scopus, and Web of Science was performed to identify rs-fMRI alterations in BPD. A total of 15 studies met our inclusion criteria. Overall, aberrant resting-state functional connectivity (rs-FC) within and between default mode network (DMN), salience network (SN), and central executive network (CEN) were observed in BPD compared to healthy controls, as well as selective functional impairments in bilateral amygdala, anterior and posterior cingulate cortex, hippocampus, and prefrontal cortex. The observational design, small sample size, prevalence of females, high rates of concurrent comorbidities and medications, and heterogeneity across imaging methodologies limit the generalizability of the results. The identification of altered patterns of rs-FC within and between selective brain networks, including DMN, SN, and CEN, could further our knowledge of the clinical symptoms of BPD, and therefore, future studies with multimodal methodologies and longitudinal designs are warranted to further explore the neural correlates of this disorder. • The functional neurobiological underpinnings of BPD are largely unknown. • This review summarizes fMRI studies exploring rs-FC in BPD. • fMRI studies show alterations in rs-FC within and between brain networks. • Results need to be replicated in larger and more homogeneous samples. • Further studies with a longitudinal design and multimodal imaging are warranted. [ABSTRACT FROM AUTHOR]

Non-small-cell lung cancer (NSCLC) remains one of the leading causes of death worldwide. While NSCLCs possess antigens that can potentially elicit T cell responses, defective tumor antigen presentation and T cell activation hinder host anti-tumor immune responses. The NSCLC tumor microenvironment (TME) is composed of cellular and soluble mediators that can promote or combat tumor growth. The composition of the TME plays a critical role in promoting tumorigenesis and dictating anti-tumor immune responses to immunotherapy. Dendritic cells (DCs) are critical immune cells that activate anti-tumor T cell responses and sustain effector responses. DC vaccination is a promising cellular immunotherapy that has the potential to facilitate anti-tumor immune responses and transform the composition of the NSCLC TME via tumor antigen presentation and cell–cell communication. Here, we will review the features of the NSCLC TME with an emphasis on the immune cell phenotypes that directly interact with DCs. Additionally, we will summarize the major preclinical and clinical approaches for DC vaccine generation and examine how effective DC vaccination can transform the NSCLC TME toward a state of sustained anti-tumor immune signaling. [ABSTRACT FROM AUTHOR]

One of the critical assessments in determining the functionality of a device is the issue of reliability in the context of the trap charges at the semiconductor and oxide interface. This article presents the results of a thorough investigation into the impact of interface trap charges (ITCs) on graphene-based channel double-gate dual-material gate graphene nanoribbon vertical tunnel field-effect transistors (DG-DMG-GNR-VTFETs). The proposed device, which takes into account GNR channel material, is compared to the conventional material, a silicon-based channel (DG-DMG-Si-VTFET), in the presence (positive/negative) or absence of trap charges, with the exact dimension specification. This work aims to assess the linearity of the proposed device and the dependability of devices with the influence of ITCs on the DC aspect, low power, and high-frequency parameters. Because of the special properties of graphene, it was found that the DG-DMG-GNR-VTFET performs better than the DG-DMG-Si-VTFET. The simulation in this work was performed using the Silvaco ATLAS TCAD device simulator. [ABSTRACT FROM AUTHOR]

Abstract Background Monochorionic (MC) twins present a higher incidence of unfavorable clinical perinatal outcomes than dichorionic (DC) twins, often in association with placental vascular anastomosis. In this study, we profiled the umbilical cord plasma metabolomes of uncomplicated MC and DC twin pregnancies and related these to several offspring outcomes, previously associated with birthweight. Methods Umbilical vein blood samples were collected at birth from 25 pairs of uncomplicated MC twins and 24 pairs of uncomplicated DC twins. The samples were subjected to gas chromatography-mass spectrometry-based metabolomics. 152 metabolites were identified from the cord plasma samples of MC and DC twins. Partial least squares discriminant analysis and pathway analysis were performed to compare within DC/MC twin pairs and between DC and MC twins. A generalized estimating equation (GEE) model was utilized to explore the correlation between metabolic differences and birthweight discordance within and between twin pairs. Results Our study revealed clear differences between the metabolite profiles of umbilical cord plasma of MC and DC twins. Metabolite profiles in MC within twin pairs and DC within twin pairs were characterized by the differences in 2 − hydroxyglutaramic acid levels and nicotinamide levels, respectively. The metabolic pathways of GSH, tryptophan, and fatty acid metabolism, were significantly downregulated in MC twins compared to DC twins. In addition, the concentration of caffeine and decamethyl-cyclopentasiloxane (D5) was positively correlated with birthweight in MC and DC twins. Conclusion This study demonstrated that the altered metabolites in umbilical plasma made contributions to the different chorionicities between uncomplicated MC twins and DC twins. The chorionicity of twins seems to affect the metabolic cross-talk between co-twin pairs and be related to birthweight discordance of twins.

The effect of electric field/current on creep deformation was examined in fine-grained 8 mol% Y 2 O 3 stabilized cubic ZrO 2 (8Y-CSZ) under direct and alternative current (DC and AC) conditions. Even at similar sample temperature of 1160–1170 °C, although the electric fields/currents accelerate the deformation of 8Y-CSZ, the acceleration effect (athermal effect), which cannot be explained by an increase of the sample temperature due to Joule heating, is much pronounced in AC than in DC. Under the deformation without the electric field/current, the creep behavior can be characterized by diffusional creep processes with a stress exponent of n ≈ 1, whereas under DC and AC, the predominant mechanism changes to grain boundary sliding (GBS) with n ≈ 2. This indicates that the athermal effect under the electric field/current changes the deformation mechanism from diffusional creep to GBS mechanisms by enhancing GBS and its rate controlling process of cation diffusivity, especially in AC. [ABSTRACT FROM AUTHOR]

Background: Monochorionic (MC) twins present a higher incidence of unfavorable clinical perinatal outcomes than dichorionic (DC) twins, often in association with placental vascular anastomosis. In this study, we profiled the umbilical cord plasma metabolomes of uncomplicated MC and DC twin pregnancies and related these to several offspring outcomes, previously associated with birthweight. Methods: Umbilical vein blood samples were collected at birth from 25 pairs of uncomplicated MC twins and 24 pairs of uncomplicated DC twins. The samples were subjected to gas chromatography-mass spectrometry-based metabolomics. 152 metabolites were identified from the cord plasma samples of MC and DC twins. Partial least squares discriminant analysis and pathway analysis were performed to compare within DC/MC twin pairs and between DC and MC twins. A generalized estimating equation (GEE) model was utilized to explore the correlation between metabolic differences and birthweight discordance within and between twin pairs. Results: Our study revealed clear differences between the metabolite profiles of umbilical cord plasma of MC and DC twins. Metabolite profiles in MC within twin pairs and DC within twin pairs were characterized by the differences in 2 − hydroxyglutaramic acid levels and nicotinamide levels, respectively. The metabolic pathways of GSH, tryptophan, and fatty acid metabolism, were significantly downregulated in MC twins compared to DC twins. In addition, the concentration of caffeine and decamethyl-cyclopentasiloxane (D5) was positively correlated with birthweight in MC and DC twins. Conclusion: This study demonstrated that the altered metabolites in umbilical plasma made contributions to the different chorionicities between uncomplicated MC twins and DC twins. The chorionicity of twins seems to affect the metabolic cross-talk between co-twin pairs and be related to birthweight discordance of twins. [ABSTRACT FROM AUTHOR]

A utility's water main repair was a triple‐bottom‐line success, thanks to having a plan in place that considered efficiency, costs, and customer service, allowing the utility to respond quickly to unforeseen events. [ABSTRACT FROM AUTHOR]

The diverse populations of tissue-resident and transitory T cells present in the skin share a common functional need to enter, traverse, and interact with their environment. These processes are largely dependent on the regulated expression of adhesion molecules, such as selectins and integrins, which mediate bidirectional interactions between immune cells and skin stroma. Dysregulation and engagement of adhesion pathways contribute to ectopic T-cell activity in tissues, leading to the initiation and/or exacerbation of chronic inflammation. In this paper, we review how the molecular interactions supported by adhesion pathways contribute to T-cell dynamics and function in the skin. A comprehensive understanding of the molecular mechanisms underpinning T-cell adhesion in inflammatory skin disorders will facilitate the development of novel tissue-specific therapeutic strategies.

In Washington, DC, Black residents have experienced unprecedented levels of cultural and physical displacement since 2000. Because of gentrification, the first “chocolate city,” long been defined by its blackness, has experienced shifts in the economy and commitments by the local government, that privilege policies that facilitate the displacement of Black families. Everyday struggles against gentrification have been of wide-ranging theoretical concern and pose an ongoing challenge for scholars in geography to understand the ways people resist gentrification and displacement. In this article, I show through an analysis of the anti-gentrification movement, #DontMuteDC, how Black people challenge the processes of gentrification by reclaiming space and resisting capitalist dispossession through cultural production. I demonstrate the relationship between Black sound aesthetics, gentrification, and a spatial politics of reclamation. I analyze the movement’s emphasis on go-go music as part of a process to (re)claim their place in the city, which I argue disrupts structures governing and managing normative space. I propose reclamation aesthetics as an analytic through which we can understand Black cultural production and Black place- and space-making practices as responses to socio-spatial inequities.

By ignoring the fire damage that would result from a nuclear attack and taking into account blast damage alone, US war planners were able to demand a far larger nuclear arsenal than necessary. [ABSTRACT FROM AUTHOR]

The demand and necessity of direct current (DC) has been increasing owing to large capacity and long distance power transmission. DC application of TP (thermal plastic) materials, which can increase the power transmission capacity compared to existing insulation materials, has been the primary focus of existing studies. This study presents the development of a highly reliable accelerated aging system that can implement the aging conditions of DC power cables. Moreover, we verify its reliability by testing TP model cables and analyzing the aging characteristics. Accordingly, we developed an accelerated aging system that can simulate thermal, electrical, and mechanical stresses collectively. We used the accelerated aging system to perform an accelerated aging test of the TP model cable for 40 cycles. The control precision of the accelerated aging system during the test was numerically calculated. The system was evaluated by using the conduction current and chemical characteristics of the aged TP model cables. The experimental results show that the precision of accelerated aging system is 1% for 1 cycle. The conduction current of the aged TP model cables was increased by more than 100% before aging, thereby it is verified the performance of the system accelerates the aging of power cables. It is expected that the highly reliable accelerated aging system of power cables can be used to perform long-term reliability tests and as diagnostic technology during the development of DC power cables.

This article discusses the current state of DC HVDC technology. The article describes the history of DC HVDC technology, starting with its early development as a voltage source, and then discusses the introduction of line-cutting into current designs. The article discusses control and co-ordination of transformers, as well as the need for a DC breaker to facilitate control of multiple plants. Recent developments in the design of DC circuit breakers are discussed. The importance of reliability is recognized, especially in relation to cables, and the issues surrounding cable design are explained. Ongoing and planned installations of VHF-VHF DC installations are described.

ABSTRACT Innate lymphoid cells (ILCs) are mainly resident in mucosal tissues such as gastrointestinal tract and respiratory tract, so they are closely linked to the microbiota. ILCs can protect commensals to maintain homeostasis and increase resistance to pathogens. Moreover, ILCs also play an early role in defense against a variety of pathogenic microorganisms including pathogenic bacteria, viruses, fungi and parasites, before the intervention of adaptive immune system. Due to the lack of adaptive antigen receptors expressed on T cells and B cells, ILCs need to use other means to sense the signals of microbiota and play a role in corresponding regulation. In this review, we focus on and summarize three major mechanisms used in the interaction between ILCs and microbiota: the mediation of accessory cells represented by dendritic cells; the metabolic pathways of microbiota or diet; the participation of adaptive immune cells.

Invariant natural killer (iNKT) cells are among the first innate immune cells to elicit early protective immunity that controls invading viral pathogens. The role of the iNKT cell subsets iNKT1, iNKT2, and iNKT17 in herpesvirus immunity remains to be fully elucidated. In this study, we examined the protective role of cornea-resident iNKT cell subsets using the mouse model of ocular herpesvirus infection and disease. Wild-type (WT) C57BL/6 (B6) mice and CD1d knockout (KO) mice were infected ocularly with herpes simplex virus 1 (HSV-1) (strain McKrae). Cornea, spleen, and liver were harvested at 0, 2, 5, 8, and 14 days postinfection (p.i.), and the frequency and function of the three major iNKT cell subsets were analyzed and correlated with symptomatic and asymptomatic corneal herpesvirus infections. The profiles of 16 major pro- and anti-inflammatory cytokines were analyzed in corneal lysates using Western blot and Luminex assays. Early during ocular herpesvirus infection (i.e., day 2), the gamma interferon (IFN-γ)-producing PLZFloRORγtlo (promyelocytic leukemia zinc finger, retinoic acid-related orphan receptor gT) iNKT1 cell subset was the predominant iNKT cell subset in infected asymptomatic corneas. Moreover, compared to the asymptomatic corneas of HSV-1-infected WT mice, the symptomatic corneas CD1d KO mice, with iNKT cell deficiency, had increased levels of the inflammatory cytokine interleukin-6 (IL-6) and decreased levels of IL-12, IFN-γ, and the JAK1, STAT1, NF-κB, and extracellular signal-regulated kinase 1/2 (ERK1/2) pathways. Our findings suggest that IFN-γ-producing PLZFloRORγtlo iNKT1 cells play a role in the protective innate immune response against symptomatic ocular herpes.IMPORTANCE We investigated the protective role of iNKT cell subsets in asymptomatic ocular herpesvirus infection. We found that early during ocular herpesvirus infection (i.e., on day 2 postinfection), IFN-γ-producing PLZFloRORγtlo iNKT1 cells were the predominant iNKT cell subset in infected corneas of asymptomatic B6 mice (with little to no corneal herpetic disease), compared to corneas of symptomatic mice (with severe corneal herpetic disease). Moreover, compared to asymptomatic corneas of wild-type (WT) B6 mice, the symptomatic corneas of CD1d KO mice, which lack iNKT cells, showed (i) decreases in the levels of IFN-γ and IL-12, (ii) an increase in the level of the inflammatory cytokine IL-6; and (iii) downregulation of the JAK1, STAT1, NF-κB, and ERK1/2 pathways. The findings suggest that early during ocular herpesvirus infection, cornea-resident IFN-γ-producing PLZFloRORγtlo iNKT1 cells provide protection from symptomatic ocular herpes.