1. Functional Expression and Cellular mRNA Localization of a G Protein-Activated K+inward Rectifier Isolated from Rat Brain
- Author
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E. Dissmann, A. Spauschus, Christine Karschin, D. von Pfeil, Andreas Karschin, and Erhard Wischmeyer
- Subjects
DNA, Complementary ,Potassium Channels ,Structural similarity ,G protein ,Xenopus ,Protein subunit ,Molecular Sequence Data ,Biophysics ,Transfection ,Biochemistry ,Cell Line ,Open Reading Frames ,GTP-Binding Proteins ,Chlorocebus aethiops ,Animals ,Amino Acid Sequence ,RNA, Messenger ,G protein-coupled inwardly-rectifying potassium channel ,Potassium Channels, Inwardly Rectifying ,Rats, Wistar ,Receptor ,Molecular Biology ,In Situ Hybridization ,Gene Library ,Base Sequence ,Sequence Homology, Amino Acid ,biology ,Inward-rectifier potassium ion channel ,cDNA library ,Brain ,Cell Biology ,biology.organism_classification ,Molecular biology ,Recombinant Proteins ,Rats ,Cell biology ,G Protein-Coupled Inwardly-Rectifying Potassium Channels ,Oocytes ,Female - Abstract
We have cloned by homology screening from a rat brain cDNA library a GIRK3-type (Kir 3.3) inwardly rectifying K + channel subunit with high structural similarity to other subfamily members whose activity is thought to be controlled by receptor-stimulated G proteins. When heterologously expressed both in Xenopus oocytes and in mammalian COS-7 cells, rbGIRK3 subunits individually fail to form functional channels. In contrast, when coexpressed with other GIRK subunits, rbGIRK3 gives rise to prominent currents which are enhanced by the stimulation of coexpressed 5-HT 1A receptors. In-situ hybridizations show that of all GIRK subunits rbGIRK3 is most widely distributed and strongly expressed throughout the rat brain and thus may play an important role in central signal processing.
- Published
- 1996
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