1. Degradation of Antimicrobial Histatin-variant Peptides in Staphylococcus aureus and Streptococcus mutans
- Author
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J. Groenink, D. Lowies, E.C.I. Veerman, W. van 't Hof, A.L.A Ruissen, A.V. Nieuw Amerongen, and Orale Biochemie (OUD, ACTA)
- Subjects
0301 basic medicine ,Staphylococcus aureus ,Time Factors ,Antimicrobial peptides ,Peptide ,Histatins ,Cysteine Proteinase Inhibitors ,medicine.disease_cause ,Microbiology ,Streptococcus mutans ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Humans ,Protease Inhibitors ,Salivary Proteins and Peptides ,General Dentistry ,chemistry.chemical_classification ,biology ,Chemistry ,030206 dentistry ,Antimicrobial ,biology.organism_classification ,Cystatins ,Peptide Fragments ,Anti-Bacterial Agents ,Amino acid ,030104 developmental biology ,Biochemistry ,Histatin ,Salivary Cystatins ,Electrophoresis, Polyacrylamide Gel ,Bacteria - Abstract
Histidine-free variants of salivary histatin 5 have a broad antimicrobial activity against various bacteria. In relation to a possible therapeutic application, we were interested in the susceptibility of these small peptides (14 amino acids long) to microbial proteinases and whether this affects their antimicrobial activity. Analyses by SDS-PAGE of supernatants of peptide-bacteria incubation showed a reduction in protein bands within 15 minutes’ incubation, as a result of cellular internalization. Degradation products of dhvar1 and dhvar2 appeared within one hour in the supernatants of Streptococcus mutans and Staphylococcus aureus. In contrast, the variants dhvar3 and dhvar4 were more resistant to degradation under the same conditions. MALDI-TOF analyses identified cleavage of dhvar1 and dhvar2 at Glu6. The N-terminal peptide part (1–6) of dhvar1 and 2 showed no bactericidal activity, while peptide fragment (7–14) showed a highly reduced bactericidal activity.
- Published
- 2003
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