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1. Infoeduk@ - Informational-educational system

Author

B.V. Koracak and D. Kacian

Subjects
Computer science, E-learning (theory), Modular system, ComputingMilieux_COMPUTERSANDEDUCATION, Information system, Mathematics education, Computer aided instruction, Educational systems
Abstract

All educational institutions have their own educational systems. These are essentially alike, but there are always differences which require that the institutionpsilas information system be adapted to its specific end-user needs. Adapting the information system of the College of Safety to the needs of their students and teachers, the Centre for Information Systems Ltd. (Centar za informacijske sustave d.o.o.) has developed Infoeduk@, a modular system which includes administrative information and education in a single location.

Published
2008
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2. Bioluminescence, chemiluminescence

Author

A. Dwenger, C. Beychok, G. Schweitzer, H. C. Pape, G. Röllig, M. L. Nerlich, E. Jonas, M. Funck, T. Zimmermann, S. Albrecht, R. Schuster, G. Lauschke, W. Jaroß, V. Kaever, E. Schmitz, K. Resch, H. Brandl, W. -D. Böhm, R. Beckert, E. Köstler, M. Menschikowski, D. Kacian, T. Lawrence, M. Sanders, J. Putnam, M. Majlessi, S. McDonough, T. Ryder, J. J. Santana Rodríguez, Z. Sosa Ferrera, A. Afonso Perera, and V. González Díaz

Subjects
Inorganic Chemistry, Posters, Biochemistry, Physical Chemistry, Analytical Chemistry
Published
1990

4. Contents, Vol. 14, 1975

Author

T. Gedde-Dahl, P.M Conneally, G.R. Douglas, J. Prosser, E. Grace, J.L. Hamerton, K.-H. Grzeschik, M.A. Ferguson-Smith, P.L. Pearson, G. Corneo, E.A. Nichols, G.J. Wullems, M. Bobrow, N.C. Sun, J.E. Syrett, J.K. McDougall, R. Rebourcet, J. Frézal, V.A. McKusick, F.H. Ruddle, W. Keijzer, A. Westerveld, T.G. Gedde-Dahl, R.M. Baker, R.C. Miller, F. Hecht, P. Goodfellow, B. Olaisen, J. Ott, A.J.J. Reuser, A. Vust, C.W.H. Partridge, D.R. Bolling, J. Fleming, S.E. Gardiner, P.J. McAlpine, D.M. Steffensen, E.R. Giblett, D. Kacian, E.M. Wurzer-Figurelli, R.S. Kucherlapati, T.B. Shows, K. Berg, H. Bosker, K.C. Atwood, M.C. Rattazzi, D.W. Buck, K.W. Jones, A.G.J.M. van der Linden, R.P. Clayton, J. German, W. Prensky, V. Niewczas-Late, D. Bergsma, C.M. Giles, D.A. Meyers, E.M. Eicher, D.S. Borgaonkar, D. Mutton, K.P. Glen, P.M. Ellis, K. Hirschhorn, E. Jones, S. Povey, A. Hoogeveen, J. van der Horst, J.J. van Rood, P.M. Price, B. Bengtsson, W.G. Burgerhout, C.C. Chang, L.R. Weitkamp, R.E. Giles, E. Magenis, H. van Someren, H.M.A. Beyersbergen van Henegouwen, R.A. Buckland, K. Willecke, U. Francke, D. Bootsma, C. Partridge, R. Kennett, E. Johnston, D.A. Hopkinson, A.E. Greene, O. Mutchinick, R.P. Creagan, R.S.K. Chaganti, L. Komarnicki, T. Mohandas, M. Fellous, K. Fenger, J.H. Edwards, G. Beckman, A.S. Henderson, M.L. Rivas, L. Beckman, W.R.T. Los, L.L. Coriell, I. Purdom, N. van Cong, P. Meera Khan, H.A. de Wit-Verbeek, G.B. Côté, A.R. Mitchell, Veronica van Heyningen, S.A. Sørensen, L. Wang, E. Solomon, J.A. Brown, W.S. Volkers, H. Galjaard, J.E. Anderson, C. Pangalos, A.P.M. Jongsma, R. Sanger, S.M. Elsevier, E. Lovrien, A.B. Bijnen, M.K. Fagerhol, J.A. Pierce, M. Lewis, E.H.Y. Chu, W.B. Bias, D. Weil, S. Chen, J. de Wit, C.G. Palmer, P.W. Teesdale, F.A. McMorris, G. Miller, A.D. Merritt, R.G. Davidson, J.R. Gosden, I. Schreuder, W.F. Bodmer, P.J.L. Cook, J.E. Gray, N. Busby, H. Kaita, R.C. Elston, Y.-S. Teng, S.M. Bowser Riley, R.A. Oosterbaan, P.L. Townes, E.B. Robson, H.J. Evans, L.E. Nijenhuis, and A. Hagemeijer

Subjects
Botany, Genetics, Biology, Molecular Biology, Genetics (clinical)
Published
1975
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5. References to the committee reports

Author

L.R. Weitkamp, J.E. Anderson, F.A. McMorris, L. Komarnicki, O. Mutchinick, S.M. Elsevier, T. Mohandas, J.L. Hamerton, G. Miller, J.R. Gosden, E. Lovrien, D.R. Bolling, J. Fleming, H.J. Evans, G.J. Wullems, J. Prosser, N.C. Sun, H. Bosker, K.C. Atwood, W. Keijzer, H.M.A. Beyersbergen van Henegouwen, A.B. Bijnen, M.K. Fagerhol, J. Ott, J. van der Horst, E. Grace, C.W.H. Partridge, R.C. Miller, Y.-S. Teng, P.J. McAlpine, J.E. Gray, J. de Wit, H. van Someren, K. Willecke, K. Hirschhorn, V. Niewczas-Late, T.B. Shows, D.A. Hopkinson, A.E. Greene, L. Beckman, J.A. Pierce, H.A. de Wit-Verbeek, P.W. Teesdale, R. Kennett, E. Johnston, R.E. Giles, G.B. Côté, P.L. Pearson, E. Magenis, A.G.J.M. van der Linden, M. Bobrow, V.A. McKusick, N. van Cong, J.H. Edwards, A.D. Merritt, P. Meera Khan, R.G. Davidson, P.L. Townes, I. Schreuder, S.A. Sørensen, K.W. Jones, J. Frézal, M.L. Rivas, E.B. Robson, D. Weil, A. Hoogeveen, R.A. Buckland, J.J. van Rood, K.-H. Grzeschik, E. Jones, E.A. Nichols, W.F. Bodmer, P.J.L. Cook, D.S. Borgaonkar, L. Wang, E. Solomon, B. Olaisen, K. Fenger, A.S. Henderson, A.J.J. Reuser, A. Vust, G. Beckman, E.M. Wurzer-Figurelli, J.E. Syrett, W.G. Burgerhout, C.C. Chang, D. Kacian, Veronica van Heyningen, S.E. Gardiner, A.R. Mitchell, W.B. Bias, C.M. Giles, S. Chen, F. Hecht, J.A. Brown, N. Busby, H. Kaita, C.G. Palmer, D.A. Meyers, R.C. Elston, T.G. Gedde-Dahl, R.M. Baker, A.P.M. Jongsma, K. Berg, R.S. Kucherlapati, L.E. Nijenhuis, I. Purdom, R. Sanger, R.P. Clayton, U. Francke, J.K. McDougall, J. German, P.M. Ellis, S.M. Bowser Riley, P.M. Price, M. Lewis, S. Povey, E.H.Y. Chu, R.A. Oosterbaan, L.L. Coriell, P. Goodfellow, D. Mutton, K.P. Glen, C. Partridge, R.S.K. Chaganti, D. Bootsma, M. Fellous, W. Prensky, W.R.T. Los, D.M. Steffensen, G. Corneo, D.W. Buck, E.M. Eicher, A. Westerveld, B. Bengtsson, M.C. Rattazzi, F.H. Ruddle, E.R. Giblett, W.S. Volkers, R.P. Creagan, T. Gedde-Dahl, P.M Conneally, G.R. Douglas, M.A. Ferguson-Smith, H. Galjaard, C. Pangalos, R. Rebourcet, D. Bergsma, and A. Hagemeijer

Subjects
Genetics, Library science, Biology, Bioinformatics, Molecular Biology, Genetics (clinical)
Published
1975
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6. On the feasibility of mapping low-multiplicity genes by in situ by hybridization

Author

E.M. Eicher, D Kacian, K.C. Atwood, and Ann S. Henderson

Subjects
Chromosome Mapping, Nucleic Acid Hybridization, In situ hybridization, Computational biology, Biology, Cell biology, Nucleic acid thermodynamics, Hemoglobins, Genes, Genetics, Humans, Multiplicity (chemistry), Molecular Biology, Gene, Genetics (clinical)
Published
1975

7. Serological analysis of reverse transcriptase of the Mason-Pfizer monkey virus

Author

A. Yaniv, Jeffrey Schlom, Steven S. Witkin, D. Colcher, Sol Spiegelman, D. Kacian, and T. Ohno

Subjects
biology, Hepatitis B virus DNA polymerase, DNA polymerase, viruses, Viral transformation, RNA-Directed DNA Polymerase, Haplorhini, biology.organism_classification, Virology, Molecular biology, Feline leukemia virus, Reverse transcriptase, Virus, Mammary tumor virus, hemic and lymphatic diseases, Murine leukemia virus, biology.protein, Animals, Macaca, RNA Viruses, Serotyping, Oncogenic Viruses
Abstract

Antiserum prepared against partially purified DNA polymerase from Mason-Pfizer monkey virus neutralized the endogenous DNA polymerase of that virus and of X381, an agent morphologically indistinguishable from MPMV, isolated from cultures prepared from the lactating mammary gland of a rhesus monkey. The antiserum did not inhibit the DNA polymerase activities of avian myeloblastosis virus, feline leukemia virus, Rauscher murine leukemia virus, Friend murine leukemia virus, murine mammary tumor virus, and simian sarcoma virus-1.

Published
1974

12. Investigation of transcription-mediated amplification as a rapid test method for Enterococci in recreational water.

Author

Walkley N, Moore D, McGee C, Kacian D, and Weeks I

Subjects
Enterococcus genetics, Regression Analysis, Reproducibility of Results, Enterococcus isolation & purification, Recreation, Transcription, Genetic, Water Microbiology
Abstract

Growth-based methods for detecting faecal contamination of recreational water require at least 24 hours to yield results, which can delay detection, action and remediation, if required. Such delays can put human health at risk while closing beaches inappropriately can cause unnecessary economic losses. There is a requirement for more rapid methods to facilitate the management of pollution events. In this study, undertaken in our respective laboratories, a molecular assay based on transcription-mediated amplification (TMA) technology was compared with established growth-based methods. The assays were used to quantitate enterococcal faecal indicator bacteria (relative to the legislative threshold of 104 colony forming units/100 mL) in 138 water samples collected from 41 different locations, representative of different recreational water types routinely sampled in Southern California. The results demonstrate the efficacy of the TMA assay for the detection of Enterococcus species in recreational water as a rapid alternative to traditional methods.

Published
2008
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13. Rapid tests for detection and quantitation of Enterococcus contamination in recreational waters.

Author

Morgan R, Morris C, Livzey K, Hogan J, Buttigieg N, Pollner R, Kacian D, and Weeks I

Subjects
Cell Extracts analysis, In Situ Hybridization, Fluorescence methods, Nucleic Acid Amplification Techniques methods, Oligonucleotide Probes analysis, Polymerase Chain Reaction methods, RNA, Ribosomal metabolism, Sensitivity and Specificity, Transcription, Genetic, Enterococcus isolation & purification, Feces microbiology, Recreation, Water Microbiology, Water Supply analysis
Abstract

Presently, growth-based tests are used for the detection and quantitation of microbiological contaminants in the environment. These tests take a minimum of 24 h to generate a result, which compromises the ability to take the most appropriate action. This report describes a rapid test for Enterococcus in recreational water as an indicator of faecal contamination. This method involves (1) isolation and lysis of the target organism, (2) purification of ribosomal RNA (rRNA) from the lysate and (3) amplification and detection of the purified rRNA. rRNA is used as the target since, in contrast to DNA, there are hundreds to thousands of copies in the cell. The rRNA is purified from the lysate by target capture onto magnetic microspheres, which removes interfering substances present in the sample. The rRNA is then quantitated using transcription-mediated amplification (TMA) with real-time homogeneous detection of amplicon using a fluorescent oligonucleotide probe. Compared to polymerase chain reaction (PCR) amplification, TMA is isothermal, more rapid, and ideally suited to RNA detection. The test described here demonstrates sensitive detection and quantitation of enterococci over a wide dynamic range with a high level of analytical specificity. The latter is particularly important for accurate and relevant monitoring both for protecting public health and for source tracking. Many conventional microbiological tests are time-consuming, exhibit limited dynamic range and are known to lack specificity. This assay demonstrates the advantages achievable by the application of TMA of rRNA targets to current environmental testing challenges.

Published
2007
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14. Bioluminescence, chemiluminescence.

Author

Dwenger A, Beychok C, Schweitzer G, Pape HC, Röllig G, Nerlich ML, Jonas E, Funck M, Zimmermann T, Albrecht S, Schuster R, Lauschke G, Jaroß W, Kaever V, Schmitz E, Resch K, Brandl H, Böhm WD, Beckert R, Köstler E, Menschikowski M, Kacian D, Lawrence T, Sanders M, Putnam J, Majlessi M, McDonough S, Ryder T, Santana Rodríguez JJ, Sosa Ferrera Z, Afonso Perera A, and González Díaz V

Published
1990
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16. Regulation of globin synthesis in the thalassemias.

Author

Bank A, Gambino R, Ramirez F, Maniatis G, Natta C, Kacian D, Spiegelman S, and Marks PA

Subjects
Animals, Carcinoma, Krebs 2 metabolism, Cell-Free System, Humans, Nucleic Acid Hybridization, Polyribosomes metabolism, Protein Biosynthesis, RNA, Messenger metabolism, RNA, Ribosomal metabolism, Rabbits, Reticulocytes metabolism, Thalassemia blood, Globins biosynthesis, Thalassemia metabolism
Published
1974
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17. A limited number of globin genes in human DNA.

Author

Gambino R, Kacian D, O'Donnell J, Ramirez F, Marks PA, and Bank A

Subjects
Base Sequence, Centrifugation, Density Gradient, Chromatography, Haploidy, Humans, Hydroxyapatites, Kinetics, Nucleic Acid Hybridization, RNA, Messenger isolation & purification, Spleen analysis, Thalassemia metabolism, DNA analysis, Genes, Globins biosynthesis
Abstract

The number of globin genes in human cells was determined by hybridizing DNA from human spleens to (3)H-labeled DNA complementary to human globin mRNA. Assuming the rates of reannealing of complementary DNA and cellular DNA are similar, the extent of hybridization of complementary DNA at various ratios of cellular DNA to complementary DNA indicate that there are fewer than 10 globin gene copies per haploid human genome. An alternative analysis of the data, which introduces no assumptions concerning the relative rates of reaction of complementary DNA and cellular DNA, indicates fewer than 20 globin gene copies are present. DNA isolated from the spleen of a patient with beta(+) thalassemia contained a number of globin gene copies similar to that of normal DNA.

Published
1974
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21. Nucleotide sequence analysis of RNA synthesized from rabbit globin complementary DNA.

Author

Poon R, Paddock GV, Heindell H, Whitcome P, Salser W, Kacian D, Bank A, Gambino R, and Ramirez F

Subjects
Alkaline Phosphatase, Amino Acid Sequence, Animals, Base Sequence, DNA, Phosphoric Diester Hydrolases, Phosphorus Radioisotopes, RNA biosynthesis, RNA-Directed DNA Polymerase, Rabbits, Ribonucleases, Templates, Genetic, Globins, RNA analysis, RNA, Messenger analysis
Abstract

Rabbit globin complementary DNA made with RNA-dependent DNA polymerase (reverse transcriptase) was used as template for in vitro synthesis of (32)P-labeled RNA. The sequences of the nucleotides in most of the fragments resulting from combined ribonuclease T(1) and alkaline phosphatase digestion have been determined. Several fragments were long enough to fit uniquely with the alpha or beta globin amino-acid sequences. These data demonstrate that the cDNA was copied from globin mRNA and contained no detectable contaminants.

Published
1974
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