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Long distance transportation can be a significant source of stress to cattle and is associated with increased risk of bovine respiratory disease (BRD). The administration of a nonsteroidal anti-inflammatory drug (NSAID) has been shown to reduce stress following long distance transport. The objective of this study was to compare performance, health, accelerometer activity, and well-being between calves receiving either tildipirosin (Zuprevo 18%; Merck Animal Health, Madison, NJ) alone or in combination with transdermal flunixin (BANAMINE Transdermal Pour-on Solution; Merck Animal Health, Madison, NJ) on arrival at the feedlot. Three hundred eighty-four polled, Continental × English, and English crossbred bulls (n = 199) and steers (n = 185) were enrolled into one of two treatments: 1) tildipirosin administered in the neck as a single dose of 4 mg/kg only (PLBO) 2) tildipirosin in combination with transdermal flunixin applied to the dorsal midline at a dose of 3.3 mg/kg (FTD). Outcomes measured were average daily gain (ADG), dry mater intake (DMI), gain to feed, morbidity, mortality, accelerometer activity data, and a daily visual analog scale (VAS) assessment of well-being. Body weight (BW) was determined by weighing individual animals; ADG was calculated as initial BW—final BW / total days on feed; DMI was calculated as daily pen feed allocation—feed remaining at next feeding / number of calves in the pen; and gain to feed was calculated as pen level ADG / pen level DMI. The VAS used was a 100 mm line anchored at each end by descriptors of “no pain” or “severe pain”. Statistical analysis was performed using JMP 13 computer software using pen as the experimental unit, lot number as a random variable, and treatment as a fixed variable. There was no treatment effect on DMI (P = 0.51). During the first 14 d on feed, FTD calves had a lower ADG of 0.90 kg/d compared with 1.33 kg/d in the PLBO group (P = 0.05). There were no differences observed in morbidity and mortality between groups (P = 0.29). There were no treatment differences from activity data (P = 0.19). The VAS assessment showed a significant time × treatment interaction (P

Recent research demonstrated that mid- or late-summer prescribed fires can be employed to manage sericea lespedeza (Lespedeza cuneata) infestations in the Kansas Flint Hills. The effects of prescribed fire applied during the growing season (i.e., August to October) on grazing performance of yearling cattle have not been evaluated. Native pastures (n = 18; 22 ± 4.0 ha) were grouped by watershed and assigned randomly to one of three prescribed-fire treatments: spring (7 April ± 2.1 d), summer (21 August ± 5.7 d), or autumn (2 October ± 9.9 d). Yearling beef cattle were grazed from May to August at a targeted stocking density of 280 kg live-weight/ha following prescribed-fire application. Forage biomass accumulations, soil cover, plant species composition, and root carbohydrate concentrations in four native plant species were evaluated. Total body weight (BW) gains and average daily gain were greater (P = 0.01) for cattle that grazed the spring and summer prescribed-fire treatments compared with those that grazed the autumn prescribed-fire treatment. As a result, final BW were greater (P = 0.04) in the spring and summer treatments than the autumn treatment. Conversely, forage biomass accumulations did not differ (P = 0.91) between fire regimes. Proportions of bare soil were greater (P < 0.01) in the spring treatment compared with the summer and autumn treatments, whereas proportions of litter on the soil surface were greater (P < 0.01) in summer- and autumn-burned pastures compared with spring-burned pastures. Total basal cover of graminoids and forbs did not differ (P ≤ 0.15) between prescribed fire treatments. Likewise, total basal cover of C3 or C4 perennial grasses did not differ (P ≥ 0.23) between prescribed-fire treatments. No treatment differences (P = 0.24) in root starch or root water-soluble carbohydrate concentrations in big bluestem (Andropogon gerardii), little bluestem (Schizachyrium scoparium), Indiangrass (Sorghastrum nutans), or purple prairieclover (Dalea purpurea) were detected. These data were interpreted to suggest that summer or autumn prescribed fire can be applied without reducing forage biomass accumulations, root carbohydrate concentrations in key native plant species, or considerably altering native plant populations compared with conventional spring-season prescribed fire; however, summer prescribed fire could be favored over spring or autumn prescribed fire both to maintain stocker cattle growth performance and to achieve control over sericea lespedeza.

Objective Pen and digestibility studies were conducted to analyze the effects of a high-energy programmed feeding protocol on nutrient digestibility, health, and performance of newly received growing cattle. Materials and Methods In Exp. 1, 354 heifers (initial BW = 214 ± 4 kg) consumed diets formulated to supply 0.99, 1.1, 1.21, or 1.32 Mcal of NEg/kg of DM, and each contained 40% Sweet Bran (Cargill Corn Milling, Blair, NE) on a DM basis. Treatments were offered to achieve 1.0 kg/d of ADG. In Exp. 2, 8 ruminally cannulated steers were used in a digestibility study using the diets from Exp. 1 in a 4 × 4 Latin rectangle design with 4 periods. Results and Discussion Morbidity and mortality rates were not different among treatments (P > 0.82) and nor was ADG (P ≥ 0.33). Dry matter intake decreased linearly (P 0.68) despite a linear decrease in average ruminal pH (P Implications and Applications In summary, these results indicate a high-energy programmed feeding protocol is more efficient than traditional lower-energy receiving diets based on roughages and does not negatively affect health or performance.

Effects of dietary energy level and intake of corn by-product-based diets on antibody production, acute phase protein response, stress, and immunocompetency of healthy and morbid newly received growing cattle were evaluated. Four dietary treatments were formulated to supply 0.99, 1.10, 1.21, and 1.32 Mcal NE(g)/ kg DM and were offered at 100%, 95%, 90%, and 85% of ad libitum based on 0.99/100 treatment intake, respectively. Thirty-two pens were utilized with approximately 12 animals/pen. Four animals from each pen (32/dietary treatment) were randomly selected and used to serve as a subset to monitor immune function and acute phase proteins following a split-plot design. In addition, two animals were randomly and independently selected from each pen (16/dietary treatment) and used to measure fecal cortisol metabolite. Additionally, animals removed from the pen one (M1), two (M2), or three (M3) times and classified as morbid were bled in conjunction with a healthy control (H) removed at the same time and the serum analyzed for the same parameters. A quadratic response to time (P < 0.01) was detected for haptoglobin concentrations and for antibody titers for bovine viral diarrhea type 1 (BVD-I) and infectious bovine rhinotracheitis (IBR; P < 0.01). Haptoglobin was lowest on arrival, highest on day 14, and similar to baseline levels by day 27. Titer levels for BVD-I and IBR were lowest on arrival, higher on day 14, and significantly higher on day 27. Titers for bovine viral diarrhea type 2 (BVD-II) responded linearly (P < 0.05) with lower levels on arrival and highest levels on day 27. Haptoglobin was elevated in morbid animals compared to healthy pen mates (P < 0.05). Titer levels for BVD-I and IBR were also higher in healthy animals compared to animals pulled for morbidity (P < 0.01). Fecal cortisol was higher on arrival than on day 14 (P < 0.05). Dietary treatment had no effect on any of the parameters investigated. In summary, high-energy receiving diets based on fermentable fiber from by-products can be fed to newly received growing cattle without negative effects on antibody production toward vaccines, inflammation, or overall stress. In addition, haptoglobin concentrations and titer levels for BVD-I and IBR viruses are higher in healthy animals compared to sick animals.

To improve profitability of newly arrived stressed feeder cattle, it is essential to minimize feed costs and health issues while optimizing growth performance. Effects of nucleotides (NA; PSB Complex; DSS Global LLC, McCormick, SC) in diets containing corn germ meal (CGM) or dried corn distillers grains with solubles (DDGS) on the health and performance of receiving and growing cattle were analyzed in 3 experiments. In Exp. 1, 213 crossbred heifers (BW 262 ± 67 kg) were used to compare CGM- and DDGS-containing diets and to determine the effects of NA in an 84-d trial. Treatments were a 2 × 3 factorial arrangement: 2 by-product–containing diets (CGM and DDGS) and 3 amounts of NA (0, 2, and 4 g/d). There were no effects of NA, corn by-product, or their interaction on growth performance (P ≥ 0.15). Experiment 2 evaluated performance and immunity effects of NA using 240 crossbred heifers (BW 268 ± 34 kg) in a 56-d trial. The diet contained DDGS, and treatments were 0, 2, and 4 g/d of NA. There were no effects of NA on growth performance (P ≥ 0.18) or fecal IgA concentration (P = 0.15). Experiment 3 used 4 ruminally cannulated Holstein heifers in a 4 × 4 Latin square design. Treatments were arranged as a 2 × 2 factorial: 2 by-product–containing diets (GCM and DDGS) and 2 amounts of NA (0 and 3 g/d). No treatment effects were observed for digestion. Ruminal pH was greater when NA was included (P < 0.05). Based on our results, diets can be formulated to contain 24.5% CGM (DM basis) in place of DDGS and maintain animal performance and digestibility. In our experiments, there was no benefit of supplementing NA in growing diets.

Methionine supplementation can improve immune function in transition dairy cattle. Our objective was to determine if supplemental methionine could reduce acute phase protein responses in newly received growing cattle. 384 crossbred heifers (222 kg initial BW; southeastern U.S. origin) were received in 4 truckloads (blocks) over 9 d. BW was measured at arrival. The following day (d 0) cattle were vaccinated for viral and clostridial diseases, received Draxxin (2.5 mg tulathromycin/kg BW), and were stratified within block by arrival BW to 1 of 8 pens containing 12 animals each. Cattle (in 32 total pens) were limit-fed at 2.2% of BW daily (DM basis) a ration containing 40% Sweet Bran, 34.5% dry-rolled corn, 10% corn silage, 7.5% supplement, 4% alfalfa hay, and 4% prairie hay. Within blocks, pens were assigned to 1 of 2 treatments: 0 (control) or 10 g/d Smartamine M (SM) to provide 6 g/d metabolizable methionine. Pen weights were collected weekly to adjust feed offered the following week. Individual BW and tail-vein blood samples were collected on d 0, 14, and 45. Plasma haptoglobin was measured to assess acute phase protein response. Incidences of morbidity (1.6% for control, 2.6% for SM) and mortality (0.5% for both control and SM) were low. Between d 0 and 45, no differences were observed for ADG (1.24 vs. 1.27 kg/d; control vs. SM, P = 0.55) or G:F (0.107 vs. 0.110, P = 0.28), but DMI was 1.3% greater (P < 0.01) for control than SM due to differences in diet DM. An interaction between treatment and linear effect of day was detected for plasma haptoglobin (P < 0.05); over time, haptoglobin increased more for control (2.15, 2.28, and 2.95 mg/mL at 0, 14, and 45 d) than for SM (2.35, 2.37, and 2.58 mg/mL). Supplemental methionine may alleviate acute phase protein responses in stressed receiving cattle.

Background Students with intellectual disabilities (IDs) have various learning difficulties and are at risk for school failure. Large inter-individual differences are described for reading, but it is unclear how these vary as a function of grade. The aim of this study was to examine various reading fluency, accuracy and comprehension parameters in second-to-eighth-grade Italian children with either borderline intellectual functioning (BIF) or mild ID (MID). Methods We examined 106 children with BIF (67 M and 39 F) and 168 children with MID (107 M and 61 F). The children were in the second to eighth grade and were comparable for chronological age (7 to 14 years). They were administered a battery of tests that assessed fluency and accuracy of word, pseudo-word and text reading, as well as text comprehension. Standardised scores allowed us to compare the performance of the two groups with normative values. Results Children with ID obtained generally low scores compared with normative values. Those with MID had greater difficulty than those with BIF. Furthermore, difficulty was greater for speed than for accuracy measures and for words than for pseudo-words. Difficulty (particularly in the case of reading speed) tended to be pronounced at later grades. Marked individual differences were present independently of MID-BIF subgrouping, as well as stimulus category and reading parameter. Conclusions As a group, children with ID showed difficulty in reading acquisition; the effect was greater for children with more severe ID, but large individual differences were observed in children with both BIF and MID. Relatively spared pseudo-word reading skills indicate efficient use of the grapheme-to-phoneme conversion routine. This processing mode may prove more ineffective at higher levels of schooling when even in regular orthographies such as Italian typically developing children rely on lexical activation.

Antimicrobial metaphylaxis is an important tool used for the prevention of bovine respiratory disease complex, an economically important disease that typically affects newly weaned beef calves that are marketed and transported a distance from their origin. An experiment was conducted to determine the effects of administering 2 different metaphylactic products [tulathromycin (TULA) and gamithromycin (GAMI)] on differences on core body temperature, health, and performance on 284 highly stressed stocker calves. Calves were procured and delivered (transient time = 13.5h average) from a southeastern United States order buyer in 3 truckload lots during a 6-d period in October 2011. The core body temperatures in nonmorbid calves in the TULA calves were greater compared with the GAMI calves the first 2h after administration and at multiple time points on d 4 through 6 of the experiment (P < 0.05). Calves receiving TULA experienced less morbidity (P < 0.01) and less chronicity (P < 0.05) when compared with GAMI calves. Calves in the TULA group had greater ADG (P < 0.001) from arrival to d 45 compared with calves injected with GAMI. This experiment indicated that metaphylactic products may affect core body temperature differently, which may be an important consideration when managing the health of high-risk calves. Moreover, both health and performance differences may exist among calves administered different metaphylactic products. Therefore, product selection is an important decision when managing high-risk calves.

Two experiments were conducted to determine the effects of including ammoniated wheat straw in diets for beef cattle on growth performance and digestibility. Treatments consisted of diets containing 70% (DM basis) concentrate containing 40% (DM basis) wet corn gluten feed with 30% (DM basis) roughage from wheat straw (STRW), anhydrous ammonia–treated wheat straw (AMMN), or a 50:50 prairie hay and alfalfa hay blend (CONT). Experiment 1 used 288 crossbred steer calves (271 ± 28 kg) in a randomized complete block design in which steers were fed their experimental diets for 56 d. Final BW, ADG, and G:F were greater for calves fed CONT than for those fed either STRW or AMMN (P < 0.05). Experiment 2 used 6 ruminally fistulated Holstein heifers (288 kg) in a 3 × 3 Latin square design. Heifers fed CONT consumed less (P < 0.05) DM, OM, and ADF compared with those fed AMMN and STRW. Feeding heifers CONT improved (P < 0.05) digestibility of DM and OM but decreased (P < 0.05) digestibility of ADF. Ruminal pH was lowest (P < 0.05) for heifers fed CONT. The results of this experiment suggest that wheat straw and ammoniated wheat straw are equivalent roughage sources but inferior to a 50:50 blend of alfalfa hay and prairie hay when fed to growing calves at 30% of the dietary DM.

Effects of corn processing and of dietary inclusion of wet corn gluten feed (WCGF) on growth performance and digestibility were analyzed in 2 experiments. Dietary treatments were arranged as a 2 × 2 factorial. Diets contained corn as either whole-shelled corn (WSC) or as dry-rolled corn (DRC), and they contained either 47% corn with no WCGF or 29% corn with 30% WCGF. In Exp. 1, 279 crossbred calves (230 kg) were allocated to treatments in a complete block design and were used to measure performance and digestion during a 60-d receiving period. Corn processing did not affect (P ≥ 0.31) growth performance. Inclusion of WCGF in the diet increased final BW and ADG (P = 0.03) but did not affect gain efficiency (P = 0.45). Digestibility of DM was increased (P < 0.01) by dietary inclusion of WCGF, and this response was greater in diets containing DRC than in those containing WSC (interaction P = 0.02). For Exp. 2, a digestibility trial used 5 ruminally cannulated Holstein heifers (248 ± 13 kg BW) in a 4 × 4 Latin square with the additional animal administered the same treatment sequence as another heifer. A tendency (P = 0.09) was observed for heifers fed DRC to have greater DMI than those fed WSC. Dietary WCGF inclusion increased (P ≤ 0.01) DMI. Similar to observations in Exp. 1, DM digestibility was improved by addition of 30% WCGF to diets containing DRC but not diets containing WSC (interaction P = 0.02). Ruminal pH was not affected by corn processing (P = 0.90), but it tended (P = 0.09) to be increased by dietary WCGF additions. Ruminal VFA concentrations were not different between WSC and DRC, but dietary inclusion of 30% WCGF decreased (P < 0.01) acetate concentrations and increased (P = 0.05) butyrate concentrations. Liquid passage rate from the rumen and ruminal liquid volume were not affected by corn processing or dietary WCGF inclusion (P ≥ 0.66). In summary, processing corn had no effect on steer performance, but including WCGF in the diet at 30% of DM increased gains of steers over the 60-d receiving period. Diet digestibility did not follow the same pattern observed for gains and efficiencies.

Long distance transportation can be a significant source of stress to cattle and is associated with increased risk of bovine respiratory disease (BRD). The administration of a nonsteroidal anti-inflammatory drug (NSAID) has been shown to reduce stress following long distance transport. The objective of this study was to compare production parameters, morbidity, and mortality between calves receiving either tildipirosin alone or in combination with transdermal flunixin on arrival at the feedlot. 384 head of polled, Continental x English and English crossbred bulls and steers were enrolled into one of two treatments: 1) tildipirosin only (PLBO) 2) tildipirosin in combination with transdermal flunixin (FTD). Tildipirosin was administered in the neck as a single dose of 4 mg/kg alone, or in combination with transdermal flunixin applied to the dorsal midline at the label dose of 3.3 mg/kg. Outcomes measured were performance parameters, dry mater intake and feed efficiency, morbidity and mortality, pen pulls and treatments, a daily visual analog scale (VAS) assessment and accelerometer data. Statistical analysis was performed using computer software (JMP 13, SAS Institute, Cary, NC). There were no significant differences in average lot weights between treatment groups (P = 0.415). There were no treatment effects on the determined dry matter intake (P = 0.51). There were no significant differences observed in morbidity and mortality between groups (P = 0.292). VAS pain assessment showed significant differences for time (P < 0.0001) and time by treatment interaction (P < 0.0001), with FTD calves having lower (VAS) scores. There were no significant differences between treatments for the acceleration data (P = 0.190). Calves administered transdermal flunixin had lower VAS scores indicating less pain the first 36 hours after drug application. Over the 63-d feeding period, no advantages in performance were observed following FTD administration.

Castration in weaned calves is stressful and affects profitability by reducing ADG and increasing susceptibility to disease. This study evaluated the effect of meloxicam, a nonsteroidal anti-inflammatory drug (NSAID), on performance and health of calves received as steers compared with bull calves surgically castrated on arrival at the feedlot. British × Continental bulls (n = 145) and steers (n = 113; BW = 193 to 285 kg) were transported for 12 h in 3 truckloads (d 0), weighed, and randomly assigned to receive either lactose placebo (CONT; 1 mg/kg) or meloxicam (MEL; 1 mg/kg) suspended in water and administered per os, 24 h before castration. On d 1, bulls were surgically castrated (CAST) and steers were processed without castration (STR). Combinations of CONT/MEL and CAST/STR were allocated to 24 pens (6 pens per treatment) of 8 to 14 calves each. Pen was the experimental unit. Plasma meloxicam concentrations at the time of castration (d 1) were determined by HPLC-mass spectroscopy. Pen-level ADG, DMI, and G:F were estimated using BW obtained on d 0, 14, and 28 and weigh-back of feed. Individual animals were classified as sick based on a depression score of ≥2 on a 5-point scale and a rectal temperature of ≥39.8°C. On d 0, 1, and 14, calf chute temperament was evaluated using a 4-point scale. Data were analyzed using generalized linear mixed models and survival curve analyses. Castration reduced pen ADG (P 0.45) were apparent by d 28. For all treatment groups, DMI increased with days on feed (P < 0.0001) but was less in CAST compared with STR calves (P < 0.016) throughout the study. From d 15 to 28, ADG increased (P = 0.0011) in CAST but not STR calves, and G:F decreased (P = 0.0004) in STR but not CAST calves. In CAST calves only, MEL treatment reduced the pen-level first pull rate (P = 0.04) and reduced bovine respiratory disease morbidity rate (P = 0.03). The frequency of chute escape behavior was greater on arrival and at castration in CAST vs. STR calves (P < 0.01) but not significantly different at d 14 (P = 0.22). Mean MEL concentrations at castration were no different between treated STR and CAST calves (P = 0.70). Meloxicam administration before castration in postweaning calves reduced the incidence of respiratory disease at the feedlot. These findings have implications for developing NSAID protocols for use in calves at castration with respect to addressing animal health and welfare concerns.

Bovine respiratory disease complex (BRDC) is the most important health issue in beef feeder calves. Our study was a randomized, blinded field trial to evaluate potential differences in health, production and behavior in feeder calves administered two different preventive health programs. Calves in two replicates (n=308 and n=305) were allocated to pens and then pens were randomly assigned a preventive health program. One program (Prog1) consisted of 1 injectable clostridial vaccine, 1 intranasal modified live respiratory vaccine, 1 topical and 1 oral parasiticide. The other program (Prog2) consisted of 1 injectable clostridial vaccine, 1 modified live respiratory vaccine and 1 injectable parasiticide. A greater percentage of calves in Prog1 (59.7%) experienced BRDC morbidity compared to the Prog2 program (47.8%). There were no differences between programs in mortality, case fatality, 1st treatment success or chronicity risks. The average daily gain over the entire study period for the Prog2 calves (1.23 kg) was greater than the Prog1 calves (1.16 kg). Calves administered Prog1 on average took more steps each day during the first 28 days of the study. Additionally, Prog1 calves spent more time lying down on certain days during the last 14 days of the study. During initial program administration, fewer Prog1 calves (39.8%) vocalized compared to Prog2 calves (47.8%). In this study, calves administered a program with fewer injections indicated less aversion to program administration than those administered more injections, but experienced greater morbidity and poorer performance.

Use of electronic animal identification technologies by livestock managers is increasing, but performance of these technologies can be variable when used in livestock production environments. This study was conducted to determine whether 1) read distance of low-frequency radio frequency identification (RFID) transceivers is affected by type of transponder being interrogated; 2) read distance variation of low-frequency RFID transceivers is affected by transceiver manufacturer; and 3) read distance of various transponder-transceiver manufacturer combinations meet the 2004 United States Animal Identification Plan (USAIP) bovine standards subcommittee minimum read distance recommendation of 60 cm. Twenty-four transceivers (n = 5 transceivers per manufacturer for Allflex, Boontech, Farnam, and Osborne; n = 4 transceivers for Destron Fearing) were tested with 60 transponders [n = 10 transponders per type for Allflex full duplex B (FDX-B), Allflex half duplex (HDX), Destron Fearing FDX-B, Farnam FDX-B, and Y-Tex FDX-B; n = 6 for Temple FDX-B (EM Microelectronic chip); and n = 4 for Temple FDX-B (HiTag chip)] presented in the parallel orientation. All transceivers and transponders met International Organization for Standardization 11784 and 11785 standards. Transponders represented both one-half duplex and full duplex low-frequency air interface technologies. Use of a mechanical trolley device enabled the transponders to be presented to the center of each transceiver at a constant rate, thereby reducing human error. Transponder and transceiver manufacturer interacted (P < 0.0001) to affect read distance, indicating that transceiver performance was greatly dependent upon the transponder type being interrogated. Twenty-eight of 30 combinations of transceivers and transponders evaluated met the minimum recommended USAIP read distance. The mean read distance across all 30 combinations was 45.1 to 129.4 cm. Transceiver manufacturer and transponder type interacted to affect read distance variance (P < 0.05). Maximum read distance performance of low-frequency RFID technologies with low variance can be achieved by selecting specific transponder-transceiver combinations.

In this paper, we present distribution-free tests to evaluate the effect of multiple treatments when there are a large number of repeated measurements from each subject nested in a treatment. We formulate new test statistics to account for heteroscedasticity and unbalanced designs. The asymptotic distributions for the test statistics are obtained when the repeated measurements from the same subject have long range dependence and weak dependence, respectively. The asymptotic results hold under the nonclassical setting in which the number of repeated measurements is large while the number of subjects per treatment may be small. A real application to compare cattle ear temperature profiles under different antibiotic treatments is given for illustration. Simulation studies are undertaken to compare the empirical performance of the proposed tests to commonly used methods.

One field study was conducted to evaluate serum steroid hormone profiles of steers exhibiting characteristics attributed to buller steer syndrome and to assess the potential association between buller steer syndrome and the monoamine neurotransmitter enzyme monoamine oxidase type A (MAO-A) mRNA. Whole blood serum was collected at 3 time points from 600 crossbred steers: 1) before placement on intensively stocked early summer pastures (pregrass), 2) after arrival to a commercial feedlot (feedlot arrival; BW = 403 kg), and 3) when a steer exhibited classical buller characteristics (diagnosis of buller; n = 25). The pregrass progesterone period was suppressed (P < 0.05) only in sera obtained from bullers as compared with control animals (342 vs. 673 pg/mL). From feedlot arrival to the diagnosis of buller, a reduction (P < 0.05) in progesterone occurred in bullers (349 vs. 217 pg/mL). An increase (P < 0.05) in testosterone occurred in bullers from feedlot arrival to diagnosis of a buller (7.4 vs. 21.7 pg/mL). Estradiol-17β from bullers and controls increased (P < 0.01) from pregrass to feedlot arrival. Buller and control estradiol and testosterone levels were not different at any sampling time. From the same group of steers, 10 bullers and 12 controls, MAO-A mRNA levels were analyzed in postmortem samples removed from the frontal cortex region of the brain. The MAO-A mRNA levels measured by the real-time quantitative PCR method were 74.5% greater (P = 0.03) in bullers than controls. This study indicates bullers have suppressed serum concentrations of progesterone and a potential predisposition for heightened MAO-A activity.

Five studies were conducted to evaluate the effects of lipid source on performance and health of stressed feeder cattle. A total of 332 heifers (195 +/- 2.37 kg initial BW) in trial 1 and 336 heifers (206 +/- 1.70 kg initial BW) in trial 2 were fed diets containing ground flaxseed (FLAX), rolled full-fat soybeans (SOY), or tallow (TAL) at 13, 20, or 4%, respectively (DM basis). All diets were formulated to be isonitrogenous and isocaloric. The ADG and G:F for the first 7 d and for the entire feeding period were greater (P < 0.05) for TAL and FLAX than for SOY. Percentage of animals treated and retreated for bovine respiratory disease did not differ among dietary treatments. The FLAX treatment increased (P < 0.05) total n-3 PUFA concentrations in the plasma, whereas SOY increased (P < 0.05) plasma concentrations of total n-6 PUFA. In trial 3, 18 steers were individually fed diets containing TAL and 18 steers were fed a diet containing SOY (20% of DM). In trials 4 and 5, 18 steers were individually fed diets containing TAL and 18 steers were fed diets containing FLAX (12.9% of DM). On d 14 and 17 of study 3, 4, and 5, 16 steers from each dietary treatment were injected i.v. with Escherichia coli O55:B5 lipopolysaccharide (LPS), and 2 steers from each diet were injected with saline. Rectal temperatures after LPS challenge were lower (P < 0.05) for SOY and FLAX than for TAL, and plasma TNF was greater (P < 0.05) for SOY than for TAL. Serum haptoglobin and blood fibrinogen increased and white blood cell count decreased in response to LPS, but none of these variables was affected by treatment. Although this research failed to measure an effect of lipid source on feedlot morbidity or mortality, these studies indicate that altering the source and type of dietary fatty acids may modify the immune response in stressed feeder cattle and that performance may be hindered by feeding full-fat soybeans to receiving cattle.

In this work, purification of lactoferrin from whey was performed with high recovery rate. Lactoferrin was then exploited in the preparation of food emulsions. Two tertiary emulsions, formed by olive oil, lecithin, chitosan, and lactoferrin, were compared: both the emulsions showed similar turbidity and stability. In the secondary emulsion formed by oil/lecithin/chitosan, the pH was increased to 9 before addition of lactoferrin. Then, lactoferrin was added, and the pH was stabilized above pH 9. Lactoferrin was found in amounts of 1 to 2.5 mg/ml in the multiple experiments. A fraction of the added lactoferrin was also present in a milky layer above the emulsion layer. This was, to our knowledge, the first study of emulsions made exploiting the interactions between lactoferrin and chitosan. It was noted that chitosan droplets remained soluble, although the hydrocolloid solubility occurs at pH lower than 5.9. These results showed the feasibility of manufacturing lactoferrin-based emulsions as functional foods.

The accelerated growth of individual animal identification systems is likely to generate significant amounts of data that need to be synchronized, filtered, analyzed, managed, and acted on in real time by data-mining software and animal health professionals who possess a dual understanding of beef systems production and technology associated with management information and record-keeping systems. Ultimately, the resulting information can be used seamlessly throughout a vertically coordinated production system to conduct management and animal health compliance audits, initiate timely animal and product recall measures, and reveal complex biologic and economic relations.

The concept of preconditioning calves has been around for a long time; yet, adoption of the practice has been slow. Current trends in the beef industry are consistent with management requirements and benefits of preconditioning, which likely will increase interest in the practice. This research estimated premiums received for preconditioned calves and the expected returns from a preconditioning program. Preconditioned calves sold in the fall received a premium of approximately $0.099 to $1.21/kg relative to non-preconditioned calves. Premiums were less when calves were sold in the winter, were less for heavier calves, and were less when cattle markets were strong. Based on a 45-d postweaning preconditioning program, cow-calf producers can increase returns about $14 per head when compared with the sale of calves at weaning with no preconditioning program. Returns associated with preconditioned calves in the feedlot are in the $40 to $60 range (per head; $0.154 to $0.243/kg), indicating that premiums paid for preconditioned calves will likely increase as the quality and

Effects of corn processing and of dietary inclusion of wet corn gluten feed (WCGF) on growth performance and digestibility were analyzed in 2 experiments. Dietary treatments were arranged as a 2 × 2 factorial. Diets contained corn as either whole-shelled corn (WSC) or as dry-rolled corn (DRC), and they contained either 47% corn with no WCGF or 29% corn with 30% WCGF. In Exp. 1, 279 crossbred calves (230 kg) were allocated to treatments in a complete block design and were used to measure performance and digestion during a 60-d receiving period. Corn processing did not affect (P ≥ 0.31) growth performance. Inclusion of WCGF in the diet increased final BW and ADG (P = 0.03) but did not affect gain efficiency (P = 0.45). Digestibility of DM was increased (P0.01) by dietary inclusion of WCGF, and this response was greater in diets containing DRC than in those containing WSC (interaction P = 0.02). For Exp. 2, a digestibility trial used 5 ruminally cannulated Holstein heifers (248 ± 13 kg BW) in a 4 × 4 Latin square with the additional animal administered the same treatment sequence as another heifer. A tendency (P = 0.09) was observed for heifers fed DRC to have greater DMI than those fed WSC. Dietary WCGF inclusion increased (P ≤ 0.01) DMI. Similar to observations in Exp. 1, DM digestibility was improved by addition of 30% WCGF to diets containing DRC but not diets containing WSC (interaction P = 0.02). Ruminal pH was not affected by corn processing (P = 0.90), but it tended (P = 0.09) to be increased by dietary WCGF additions. Ruminal VFA concentrations were not different between WSC and DRC, but dietary inclusion of 30% WCGF decreased (P0.01) acetate concentrations and increased (P = 0.05) butyrate concentrations. Liquid passage rate from the rumen and ruminal liquid volume were not affected by corn processing or dietary WCGF inclusion (P ≥ 0.66). In summary, processing corn had no effect on steer performance, but including WCGF in the diet at 30% of DM increased gains of steers over the 60-d receiving period. Diet digestibility did not follow the same pattern observed for gains and efficiencies.

SummaryVery-high-purity Factor VIII concentrates produced by monoclonal or recombinant technology have been postulated to be more antigenic resulting in an increased risk of inhibitor development in hemophilia A patients. However, previous reports, mainly based on prevalence figures, may have understimated the “true” risk of this complication in patients treated with less pure Factor VIII concentrates. The present study, started in 1975, has been designed to calculate the risk of inhibitor development in patients with severe or moderate hemophilia A, followed since their first exposure to intermediate or high-purity Factor VIII concentrates, produced by conventional technologies. Sixty-four hemophiliacs fulfilled the enrollment criteria. Inhibitors developed in 20.3% (13/64) of all patients and in 23% (11/48) of those with severe Factor VIII deficiency. Eleven patients manifested a strong anamnestic response after exposure to Factor VIII (high responders) and 2 had low inhibitor concenlialions despite repeated Factor Vlll infusions (low responders). The incidence of inhibitor development was 24.6 per 1000 patient yeuis of observalion. The, cumulative! risk of inhibitor formation was 19,9% at age of 6 years, and 20.3% at 5 years after the first exposure. The risk was 19.3% at 70 days of exposure to Factor VIII concentrates, and 17.2% after a total of 50,000 units of Factor VIII given.Further stuides are needed to confirm the above risk of acquiring an inhibitor, which indicates and under-estimations by previous studies. In addition, more data is needed to demonstrate whether very high purity Factor VIII concentrates may be more antigenie than conventional preparations.

Castration in weaned calves is stressful and affects profitability by reducing ADG and increasing susceptibility to disease. This study evaluated the effect of meloxicam, a nonsteroidal anti-inflammatory drug (NSAID), on performance and health of calves received as steers compared with bull calves surgically castrated on arrival at the feedlot. British × Continental bulls (n = 145) and steers (n = 113; BW = 193 to 285 kg) were transported for 12 h in 3 truckloads (d 0), weighed, and randomly assigned to receive either lactose placebo (CONT; 1 mg/kg) or meloxicam (MEL; 1 mg/kg) suspended in water and administered per os, 24 h before castration. On d 1, bulls were surgically castrated (CAST) and steers were processed without castration (STR). Combinations of CONT/MEL and CAST/STR were allocated to 24 pens (6 pens per treatment) of 8 to 14 calves each. Pen was the experimental unit. Plasma meloxicam concentrations at the time of castration (d 1) were determined by HPLC-mass spectroscopy. Pen-level ADG, DMI, and G:F were estimated using BW obtained on d 0, 14, and 28 and weigh-back of feed. Individual animals were classified as sick based on a depression score of ≥2 on a 5-point scale and a rectal temperature of ≥39.8°C. On d 0, 1, and 14, calf chute temperament was evaluated using a 4-point scale. Data were analyzed using generalized linear mixed models and survival curve analyses. Castration reduced pen ADG (P0.001) and G:F (P0.001) from d 1 to 14, yet no effects (P0.45) were apparent by d 28. For all treatment groups, DMI increased with days on feed (P0.0001) but was less in CAST compared with STR calves (P0.016) throughout the study. From d 15 to 28, ADG increased (P = 0.0011) in CAST but not STR calves, and G:F decreased (P = 0.0004) in STR but not CAST calves. In CAST calves only, MEL treatment reduced the pen-level first pull rate (P = 0.04) and reduced bovine respiratory disease morbidity rate (P = 0.03). The frequency of chute escape behavior was greater on arrival and at castration in CAST vs. STR calves (P0.01) but not significantly different at d 14 (P = 0.22). Mean MEL concentrations at castration were no different between treated STR and CAST calves (P = 0.70). Meloxicam administration before castration in postweaning calves reduced the incidence of respiratory disease at the feedlot. These findings have implications for developing NSAID protocols for use in calves at castration with respect to addressing animal health and welfare concerns.

Use of electronic animal identification technologies by livestock managers is increasing, but performance of these technologies can be variable when used in livestock production environments. This study was conducted to determine whether 1) read distance of low-frequency radio frequency identification (RFID) transceivers is affected by type of transponder being interrogated; 2) read distance variation of low-frequency RFID transceivers is affected by transceiver manufacturer; and 3) read distance of various transponder-transceiver manufacturer combinations meet the 2004 United States Animal Identification Plan (USAIP) bovine standards subcommittee minimum read distance recommendation of 60 cm. Twenty-four transceivers (n = 5 transceivers per manufacturer for Allflex, Boontech, Farnam, and Osborne; n = 4 transceivers for Destron Fearing) were tested with 60 transponders [n = 10 transponders per type for Allflex full duplex B (FDX-B), Allflex half duplex (HDX), Destron Fearing FDX-B, Farnam FDX-B, and Y-Tex FDX-B; n = 6 for Temple FDX-B (EM Microelectronic chip); and n = 4 for Temple FDX-B (HiTag chip)] presented in the parallel orientation. All transceivers and transponders met International Organization for Standardization 11784 and 11785 standards. Transponders represented both one-half duplex and full duplex low-frequency air interface technologies. Use of a mechanical trolley device enabled the transponders to be presented to the center of each transceiver at a constant rate, thereby reducing human error. Transponder and transceiver manufacturer interacted (P0.0001) to affect read distance, indicating that transceiver performance was greatly dependent upon the transponder type being interrogated. Twenty-eight of 30 combinations of transceivers and transponders evaluated met the minimum recommended USAIP read distance. The mean read distance across all 30 combinations was 45.1 to 129.4 cm. Transceiver manufacturer and transponder type interacted to affect read distance variance (P0.05). Maximum read distance performance of low-frequency RFID technologies with low variance can be achieved by selecting specific transponder-transceiver combinations.

Health and feed performance parameters of 293 beef stocker calves at risk for bovine respiratory disease were compared after metaphylactic administration of one of two antimicrobials (tulathromycin or tilmicosin) with different durations of activity; the antimicrobial was administered 1 day after arrival. Calves that received metaphylactic tulathromycin displayed significant improvement in morbidity, mortality, and first-treatment success rates (P.05) compared with tilmicosin-treated calves. Tulathromycin-treated calves also showed a significantly improved average daily gain and feed:gain ratio (P.05) compared with tilmicosin-treated calves. Under conditions of this study, calves receiving tulathromycin were healthier through a 43-day growing phase compared with calves receiving tilmicosin. This health difference likely accounted for the differences in feed performance between the treatment groups.

Five studies were conducted to evaluate the effects of lipid source on performance and health of stressed feeder cattle. A total of 332 heifers (195 +/- 2.37 kg initial BW) in trial 1 and 336 heifers (206 +/- 1.70 kg initial BW) in trial 2 were fed diets containing ground flaxseed (FLAX), rolled full-fat soybeans (SOY), or tallow (TAL) at 13, 20, or 4%, respectively (DM basis). All diets were formulated to be isonitrogenous and isocaloric. The ADG and G:F for the first 7 d and for the entire feeding period were greater (P0.05) for TAL and FLAX than for SOY. Percentage of animals treated and retreated for bovine respiratory disease did not differ among dietary treatments. The FLAX treatment increased (P0.05) total n-3 PUFA concentrations in the plasma, whereas SOY increased (P0.05) plasma concentrations of total n-6 PUFA. In trial 3, 18 steers were individually fed diets containing TAL and 18 steers were fed a diet containing SOY (20% of DM). In trials 4 and 5, 18 steers were individually fed diets containing TAL and 18 steers were fed diets containing FLAX (12.9% of DM). On d 14 and 17 of study 3, 4, and 5, 16 steers from each dietary treatment were injected i.v. with Escherichia coli O55:B5 lipopolysaccharide (LPS), and 2 steers from each diet were injected with saline. Rectal temperatures after LPS challenge were lower (P0.05) for SOY and FLAX than for TAL, and plasma TNF was greater (P0.05) for SOY than for TAL. Serum haptoglobin and blood fibrinogen increased and white blood cell count decreased in response to LPS, but none of these variables was affected by treatment. Although this research failed to measure an effect of lipid source on feedlot morbidity or mortality, these studies indicate that altering the source and type of dietary fatty acids may modify the immune response in stressed feeder cattle and that performance may be hindered by feeding full-fat soybeans to receiving cattle.

In this study label-free a SPR immunosensor and a protein array based methods were used for their application in bacterial detection. L. monocytogenes, one of the most difficult to treat bacteria, was used as model pathogen. While the use of DNA arrays for bacteria detection and identification is largely documented, no studies are available on protein array (PA) based bacteria detection. In protein array approach an affinity-purified monoclonal antibody was used as capture antibody. Protein array detection limit was of 10 2-10 3CFU/mL. SPR immunoassays were prepared by chemically binding L. monocytogenes cells on a proper gold substrate. After immobilization of antigen on gold substrate, a further incubation with anti-L. monocytogenes antibodies was performed. The technique was revealed as powerful and fast method for the monitoring of binding between the investigated antigen and antibodies. On the other side, array based methods, although more expensive due to the labelling step, can be used with a set of species-specific antibodies allowing for simultaneous detection of multiple pathogens in a single hybridization step in no more then 2.5 h.

In Exp. 1, 300 heifers (260 kg initial BW) were used to compare growth performance of cattle fed forage-free diets containing predominantly soybean hulls with that of cattle receiving roughage- and corn-based diets and to determine whether cattle fed soybean hull-based diets would respond to supplementation with methionine hydroxy analogue (MHA), lipid-coated betaine, or concentrated separator by-product (CSB; a source of betaine). Treatments included 1) a roughage-based diet fed at 2.75% of BW, 2) a corn-based diet fed at 1.5% of BW, 3) a corn-based diet fed at 2.25% of BW, 4) a soybean hull-based diet fed at 1.5% of BW (SH1.5), 5) a soybean hull-based diet fed at 2.25% of BW (SH2.25), 6) SH1.5 top-dressed with 11.4 g/d Alimet (10 g/d MHA), 7) SH2.25 top-dressed with 11.4 g/d Alimet, 8) SH2.25 top-dressed with 7 g/d of a lipid-coated betaine product (4.2 g/d betaine), and 9) SH2.25 top-dressed with 250 g/d CSB (15.5 g/d betaine). Supplemental MHA, betaine, and CSB did not change DMI, ADG, or gain:feed ratio for cattle fed soybean hulls. Heifers fed soybean hull-based diets gained 29% slower (P < 0.05) and had 27% lower gain:feed ratios than heifers fed the corn-based diets. Cattle fed soybean hull-based diets had gains that were lower (P < 0.05) than those of cattle fed the roughage-based diets, but gain:feed ratios were similar because cattle were fed less of the soybean hull-based diets. Roughage-fed cattle had similar gains but 25% lower (P < 0.05) gain:feed ratios than cattle fed the corn-based diets. In Exp. 2, degradation by ruminal microbes of betaine in anhydrous betaine, betaine-HCl, feed-grade betaine, lipid-coated betaine, and CSB was evaluated in vitro using ruminal inocula collected from steers fed a high-grain or high-roughage diet. The roughage diet led to less betaine disappearance than the grain diet. More betaine was degraded from CSB than from other sources, perhaps because sugars provided by CSB stimulated fermentation, but no large differences occurred among the other four sources. Betaine from all sources was extensively degraded, although some betaine may escape ruminal degradation.

Grazing trials were conducted utilizing individually supplemented lactating beef cows in a 2-yr study to determine the effect of supplemental escape protein (EP) on cow/calf performance during the active growth periods of smooth brome and big bluestem. Graded levels (0, .11, .23, and .34 kg of EP/animal from an equal-protein-basis mixture of blood meal and corn gluten meal were offered daily, replacing corn starch and molasses, which was used as the energy control. All cows received 582 g of supplemental DM/d. Analyses for the smooth brome study indicated a cubic response (P less than .05) to milk production and calf daily gain. No response to EP supplementation (P greater than .10) was observed for the big bluestem study. Analysis of esophageal extrusa samples collected throughout the grazing seasons for both years demonstrated that quality of forage selected by the animal decreased as the trial progressed. Using a 16-h modified dacron bag technique that did not adjust for microbial attachment, protein escaping degradation for smooth brome and big bluestem was estimated to range from 7.0 to 14.5% and 22.2 to 30.3%, respectively. There was limited response to a moderate level of EP supplementation of lactating cows grazing smooth brome, but calf gains were depressed by both the low and the high levels of EP. Lactating cows grazing big bluestem did not respond to EP supplementation, but added energy supplement depressed calf gains.

The objectives of this research were to determine 1) the effects of hydrolysis time on feather meal (FTH) protein digestion and ruminal escape and 2) whether adding blood meal (BM) to FTH evoked a complementary response in animal performance. A lamb digestion trial was conducted to estimate true protein digestibility of soybean meal (SBM), BM, and FTH hydrolyzed for 10, 12, 15, or 18 min. Ruminal escape was estimated in situ. Two 94-d growth trials were conducted using 60 growing calves (226 kg) per trial to evaluate urea, FTH, BM, and 87.5:12.5, 75:25, and 50:50 combinations (CP percentage basis) of FTH:BM. There were small numerical differences in estimated escape of protein from the rumen and DM and protein digestibilities due to hydrolysis time. True protein digestibility of the 10- and 18-min samples was 5% higher (P less than .05) than for the 12- or 15-min FTH samples. In the growth trial, the slope-ratio technique showed that the most efficiently used protein supplement was 100% BM (protein efficiency = 2.45 +/- .19). No differences (P = .30) in protein efficiency were observed among supplements containing various combinations of FTH:BM. There was a quadratic (P less than .01) response to the level of BM, indicating a complementary effect. The largest complementary effect occurred at the 12.5% level of the BM addition. There were no nutritionally important effects of hydrolysis time between 10 and 18 min. Furthermore, supplements can be formulated more economically using small amounts of BM with FTH without compromising biological efficiency.

Meningeal localization from hematological malignancies represents a rare event, associated with unfavourable prognosis. Treatment is based on the administration of high doses of drugs able to cross the hemato-encephalic barrier, such as Methotrexate (MTX) and cytarabine (ara-C), cranial and cranial-spinal irradiation and intrathecal (IT) administration of ara-C and/or MTX. These treatments often fail in producing a symptoms improvement and are loaded by an high rate of side effects or by a disconfortable schedule of administration. Depocyte® is a preparation of ara-C englobed in a lipidic multivescicular frame that allows a gradual and harmonic release of the drug for, at least, 14 days. This feature has allowed its utilization in treatment and prophylaxis of CNS localizations in hematological malignancies, being effective in both settings. In our Department. two patients with meningeal localizations from hematological malignancies, have been treated with Depocyte®. Patients characteristics are listed in the table. The former, a female 24 year-old patient, suffered from Burkitt’s lymphoma. After second cycle of therapy in accordance with CHOP scheme, she showed signs and symptoms of meningeal localizations, characterized by cephalea, diplopia with lateral deviation of right eye and, subsequently, seizures. IT treatment with Depocyte® at the dose of 50 mg every 14 days has been started, achieving a partial remission of symptoms, with a reduction of cephalea and of frequency of seizures. The patient died because of a neurological worsening of the disease and its systemic diffusion. The latter, a male 25 year-old patient, suffering from T-ALL, has shown neurological symptoms after a cycle of induction and during CR. Symptoms have been characterized by cephalea and bilateral paralysis of the VII pair of cranial nerves, as well as by dysphagia and difficult deglutition. Treatment with Depocyte® has allowed an improvement of neurological symptoms and blasts disappeared from the CSF. In spite of therapy, remission has resulted short with a relapse of bone marrow desease. Therapy with Depocyte®, in accordance with what is already reported in literature, has proved to be an effective treatment of meningeal localizations, although a complete and lasting remission has not been achieved in our patients, related mainly to the aggressiveness of the underlying disease. However, thanks to its gradual release formulation, Depocyte® allows a lower frequency of IT administrations, compared with traditional drugs; moreover, it has shown a quite good handiness and efficacy in a short-term response of reported patients. Pazient 1 Pazient 2 Diagnosis Burkitt’s lymphoma T-ALL Age/Sex 24/F 25/M Diagnosis date May 2005 October 2005 CNS Prophylaxis None I.T. MTX Type of relapse Isolated Isolated + Combined Treatment Depocyte® Depocyte® Relapse date August 2005 November 2005 Symptoms III pair of cranial nerves, diplopia, cephalea, seizures VII pair of cranial nerves, cephalea, dysphagia Response (blasts in CNS) N.E. CR Evolution Progression, death Relapse

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Net- and twine-wrapped bales were stored from July 1990 until April 1991 at three Kansas sites. Changes in dry matter (DM), acid detergent fiber (ADF), and available crude protein (ACP) levels during the storage period were not significantly different between net- and twine-wrapped bales. Although a significant difference in DM change between inside and outside storage was found at one site, it was not considered important because DM changes were small. No difference in ADF or ACP changes were found between initial samples from the full bale and those taken from the outer 10 cm (4 in.) of the bales after outside storage. Neutral detergent fiber (NDF) increases were greater (p < 0.05) in twine-wrapped bales. The NDF increase in the outer 10 cm (4 in.) of twine-wrapped bales stored outside was greater (p < 0.05) than that in net-wrapped bales. The minimal weathering loss was attributed to lower than average rainfall experienced during the storage period, high density bales, and low initial hay moisture content (m.c.) at two sites. Based on the results of this study, net wrap is not justified on the basis of reduced weathering loss. However, the trend towards higher fiber concentrations in the outer 10 cm (4 in.) of twine-wrapped bales indicates that net-wrap may reduce weathering loss when weather conditions are more severe.