Search

The aim of this research was to estimate the effects of grading up of Gentile di Puglia with Ile de France on carcass traits and the composition of cuts in lambs slaughtered at 35 and 54 days of age. Data was collected on 223 lambs of both sexes, single birth, belonging to the genetic types Ile de France (IF), Gentile di Puglia (GP) and the result of grading up of the latter with Ile de France rams (F1 and F2). About half of the subjects were fed with maternal milk (natural feeding) while the others were fed on a milk replacer (artificial feeding). The lambs of both groups were randomly allocated in two slaughter age subgroups: 35 and 56 days. The results of statistical analysis showed that the considered factors of variation significantly influences the weight of the carcass, the percentage of cuts and their composition. Most of the variation was due to the age and to the feeding system. The F2 lambs on average had the heaviest carcass and sides, the GP had the highest percentage of fore quarter while the IF had the highest percentage of back quarter. Furthermore F1 lambs did not show any heterotic effect compared with both parental breeds, while F2 breeds showed better performance. The carcass of the males resulted heavier than the females, with a lower percentage of dissectible fat at the neck, brisket, shoulder and leg and a higher percentage of meat in the shoulder cut. The lambs slaughtered at 35 days of age showed a higher incidence of kidney, shoulder and leg weight, while at 56 days furnished a higher percentage of kidney fat, brisket and loin weight. The artificially fed lambs furnished a lighter carcass and neck and shoulder cuts than naturally fed lambs, less kidney fat and a higher percentage of kidney, rib and loin weight. The employment of artificial feeding permitted a higher percentage of lean and less dissectible fat as well as a higher lean/bone ratio. These results show that Ile de France rams crossbreed with Gentile di Puglia determine good productive performance if the lambs derive from the F2 crossbreed and are artificially fed and slaughtered at 56 days of age.

The study was carried out on 86 ‘Cilentana’ goats, distributed in four herds. Milk samples were collected during morning and evening milking, and pH, dry matter, fat, protein and lactose percentage as well as the lactodynamometric profile were detected to determine enzymatic phase duration, coagulation speed and curd consistency. Polymorphism of the s1-casein protein was detected. Four alleles (designated A, B, E and F) and nine genotypes were detected. The association of these nine genotypes with chemical composition and lactodynamometric characteristics was investigated. The BF genotype was the most frequent (35.5 %), followed by EE (15.1 %) and AF and BB (10.4%). The F allele showed the highest frequency and it negatively influenced cheesemaking as it was associated with a low s1-casein content. Goats with the AB genotype produced milk with higher dry matter, protein and fat content compared with the FF genotype. Moreover, milk from the AB genotype has a lower enzymatic phase duration and a higher coagulation speed than the BB genotype. Curd consistency is higher for milk from the AA genotype, when compared with the EF genotype. All the chemical and lactodynamometric characteristics varied with herd and months of control; furthermore, the milk obtained from morning milking showed better coagulation properties.

Vengono descritti gli interventi che le regioni italiane hanno intrapreso a sostegno delle razze autoctone minacciate. Gli interventi sono riportati suddivisi per specie: bovini, ovini, caprini, equini, asinini, suini, avicoli

Vengono descritte le azioni proposte dal gruppo di lavoro ASPA sulla biodiversità animale per la salvaguardia delle risorse genetiche animali all'interno delle priorità strategiche del global plan of action.

Summary The genetic identification of the population of origin of individuals, including animals, has several practical applications in forensics, evolution, conservation genetics, breeding and authentication of animal products. Commercial high-density single nucleotide polymorphism (SNP) genotyping tools that have been recently developed in many species provide information from a large number of polymorphic sites that can be used to identify population-/breed-informative markers. In this study, starting from Illumina BovineSNP50 v1 BeadChip array genotyping data available from 3711 cattle of four breeds (2091 Italian Holstein, 738 Italian Brown, 475 Italian Simmental and 407 Marchigiana), principal component analysis (PCA) and random forests (RFs) were combined to identify informative SNP panels useful for cattle breed identification. From a PCA preselected list of 580 SNPs, RFs were computed using ranking methods (Mean Decrease in the Gini Index and Mean Accuracy Decrease) to identify the most informative 48 and 96 SNPs for breed assignment. The out-of-bag (OOB) error rate for both ranking methods and SNP densities ranged from 0.0 to 0.1% in the reference population. Application of this approach in a test population (10% of individuals pre-extracted from the whole data set) achieved 100% of correct assignment with both classifiers. Linkage disequilibrium between selected SNPs was relevant (r2 > 0.6) only in few pairs of markers indicating that most of the selected SNPs captured different fractions of variance. Several informative SNPs were in genes/QTL regions that affect or are associated with phenotypes or production traits that might differentiate the investigated breeds. The combination of PCA and RF to perform SNP selection and breed assignment can be easily implemented and is able to identify subsets of informative SNPs useful for population assignment starting from a large number of markers derived by high-throughput genotyping platforms.

Summary Constitutive heterochromatin (HC = C-banding) distribution was studied in pig (Sus 5crofa) chromosomes from 20 animals belonging to Cinta Senese and Calabrese breeds raised in southern Italy. The use of CBG-banding, sequential GBG/CBA-banding and sequential GBGA/g- NOR/CBA-banding techniques allowed more detailed characterization of C-banding patterns in pig chromosomes (SSC). The following features were noticed: (a) all autosomes and the X-chromosome showed centromeric C-positive bands; (b) the entire q-arm and proximal part of the p-arm y chromosome were C-positive: (c) clear interstitial C-positive bands were noticed in SSC1q17, SSC3p14 and SSC16q21; (d) the nucleolus organizer (NO) chromosome 10 showed two distinct HC-blocks very far apart in both arms with large, polymorphic (different size) NORs between the chromosome pair, while NO-chromosome 8 showed only one C-positive band (the smallest) in the q-arms; (e) C-band polymorphism was observed between and within chromosome pairs also in relat...

SUMMARYBiological diversity is the main measure of genetic evolution; it links to the state of genetic polymorphism as influenced by envirornmental changes and modulates the speed of transferring genetic information. The authors concentrate in this note on the importance of the contribution of indigenous animal genetic resources when addressing the complex and economically most important problem of the intrinsic quality of products of animal origin, with special reference to regional/local specificity.

A new and unusual reciprocal translocation was detected in a heifer of the Agerolese cattle breed during a routine cytogenetic screening carried out on 13 animals (2 males and 11 females) kept at the ConSDABI Conservation Center in Benevento (Southern Italy). The 13 animals investigated had a normal karyotype except for a 1-year-old female, which carried one autosome smaller than the smallest normal bovine autosomes. This small autosome showed very little C-banding in comparison to the other autosomes, while another medium-sized autosome showed 2 distinct and prominent C-bands. RBA-banding and karyotype analysis revealed that these 2 chromosomes were the result of a reciprocal translocation between chromosomes 11 and 25. FISH analysis with BAC142G06 mapping to the proximal (subcentromeric) region of both BTA25 and der11, BAC513H08 (ELN) mapping to BTA25q22dist and der25, and BAC533C11 mapping to the proximal region of BTA11 and der11 confirmed the localization of the breakpoints on band q11 (centromere) of chromosome 11 and q14–21 of chromosome 25. Ag-NOR and sequential RBA/Ag-NOR techniques detected the presence of NORs on both BTA11 and BTA25 and both der11 and der25. To our knowledge, this is the first report of a reciprocal translocation event in cattle with the breakpoint located in the centromeric region.

SUMMARYAfter a rapid review of the sociocultural aspects justifying that rare breeds be protected, of the aim of a conservation programme. and of the possible strategies, this paper describes in details the activities implemented at the National Centre for the preservation of germplasm of animals at risk of extinction, at Circello, in the south of Italy. In this centre, established on 310 ha, are presently raised 281 animals of more than 30 different breeds, among which 11 cattle breeds, 7 sheep breeds and 10 goat breeds. Activities of the centre include preservation, description, multiplication, improvement and use of the breeds at risk. Collaboration with other insttitutions within the Mediterranean region are listed.

Convegno CNR - Progetto finalizzato Biotecnologie Genova

The present study was carried out in order to set up a standardized quantitative assay for spontaneous micronuclei in bovine lymphocytes. For this purpose the cytokinesis-block micronucleus (MN) method, originally proposed by Fenech and Morley (1985) for human lymphocytes, was applied to peripheral blood lymphocytes of 20 healthy cows of Italian Friesian breed. The results demonstrate that the optimal concentration of cytochalasin B to obtain the highest frequency of binucleated cells (mean = 400.26 +/- 23.76/1000 cells scored) was 6 micrograms/ml. The baseline frequency of spontaneous MN formation in 500 binucleated cells was 12.3 +/- 4.1, i.e., 3 times higher than that reported in human lymphocytes (Fenech and Morley, 1985; Scarfi et al., 1991). The possible reason(s) for this difference (sensitivity to cytochalasin B, chromosome number, environmental genotoxic pollutants) is discussed.

the same prometaphase chromosomes of cattle, is presented with the aim of establishing correlations between G and R bands. The results of the present investigation contributed to the establishment of new standard GTG and RBA-banded karyotypes at prometaphase level, useful for the precise identification of chromosomal abnormalities, comparative cytogenetics and gene mapping in the species Bos taurus L.

R-banded prometaphase karyotypes of the goat are presented using both fluorescent and light staining techniques. A model for the standardization of the R-banded prometaphase goat karyotype is suggested.

The study was carried out on 13 muscles (Table 1) of water buffalo (Italian) and bovine (Italian Fviesian) calves raised under the same micro- environmental conditions and slaughtered at 20, 28 and 36 weeks of age (Table 2). The rheological (hardness, chewiness and water holding capacity, (WHO), chemical, (dry matter, protein, fat and ash) and colour variables (brightness and spectrophotometric curves) have been evaluated on raw meat. The more significant results were: 1) The muscle confirms its marked individuality by determining independently from age and species all the studied variables. 2) Age is determinant in varying the organoleptic characteristics — at 36 weeks the meat is harder, darker, fatter (positive variation in relation to palatability), requires more chewing energy and has more WHC (positive variation) than at 20 and 28 weeks. 3) The organoleptic characteristics of the meat of the two species vary in a similar way according to the slaughter age. 4) Within the age and considering the qualitative variables all together, the buffalo calves seem superior to the bovine and provide more uniform carcasses. 5) At this stage it seems opportune to integrate these results with breeding, slaughtering and cutting data, and to complete the slaughtering ages at 44 and 52 weeks. 6) The results confirm the usefulness of techniques employed for evaluation of organoleptic characteristics of meat in view of its technology, marketing and genetic improvement.

The eating quality of seasoned hams from eight pig genetic types was evaluated using a sensory panel. The results showed that the factors examined (genetic type, sex, ham weight category and panellist) had significant effects on the organoleptic characteristics of the ham. The effects of sex and ham weight on meat quality varied between genetic types. It was concluded that the production standards for ‘Modena’ and ‘Parma’ hams should be re-examined in light of these results to ensure a quality seasoned ham from the genetic types of pig that are in use.

G,Q and R-banding pattern comparisons between the Murrah type of Bubalus bubalis L. (2n = 50) and the Holstein Friesian breed of Bos taurus L. (2n = 60) chromosomes revealed that the autosomes are similar in both species; in the former, the five pairs of submetacentrics correspond to centric fusions of chromosomes 1-29, 2-22, 8-19, 5-28 and 16-25 in the latter. Silver staining on somatic cells of buffalo revealed telomeric Ag-NORs located on six pairs of autosomes, identified as 3p, 4p, 8, 21, 23 and 24. Only one pair of nucleolus organizer chromosomes is common to both species, namely the 4p of buffalo which corresponds to the 28 of cattle. The remaining NORs are located on different pairs. Out of 18 individuals, the number of Ag-NORs per cell varied between 3 and 10, with a mean value of 6.3 ± 1.7; the mean number of nucleolar associations per cell was between 0.1 and 1.8. In the total population of 317 cells the two variables followed the binomial and the Poisson distributions, respectively, and their correlation coefficient was not statistically significant (r = +0.36). In addition to the mean number of Ag-NORs, the mean number of nucleolar associations per cell should be used for a better understanding of the differences among individuals.

High rates of structural chromosome aberrations were associated with increased yields of sister chromatid exchanges (SCE) in metaphase chromosomes of a new born female calf affected by a congenital malformation. The frequency of abnormal cells was 25 per cent in the abnormal calf, 8 per cent in its dam and 3 per cent in a group of four healthy cows. Chromatid and chromosome breaks were the most frequent types of chromosome aberration found in the malformed calf; centric fusions, chromosome fragments and deletions were much less common. The mean rate of SCE/cell in the malformed calf was nearly twice that of the control and the difference was statistically significant. Possible factors involved in the occurrence of such a malformation are discussed.

In relazione alle frazioni dell'intervallo interparto e stata studiata l'efficienza riproduttiva nelle vacche (445 soggetti) di razza Frisona italiana, allevate in una grande azienda del Salernitano (Italia), come numero di inseminazioni per concepimento, come percentuale degli aborti sul totale di essi e sui concepimenti, e come lunghezza del periodo dal concepimento all'aborto.

Despite the growing diffusion of cosmopolite specialized breeds, several autochthonous cattle breeds and populations are still bred in Italy. The aim of this study was to provide a high-resolution picture of the genome-wide diversity and population structure of Italian local cattle breeds using medium-density genome-wide SNP markers. After data editing, the dataset included 800 samples from 32 breeds that were genotyped for 31 013 SNPs. For several breeds we observed a low level of polymorphism and genetic diversity, that together with the small effective population sizes confirmed threat of extinction. According to the runs of homozygosity analysis, evidence of recent inbreeding was strong in some breeds. Low pair-wise genetic differentiation values, shared ancestry, admixture events, and reticulations observed on the phylogenetic tree between some breeds, all suggest high levels of gene flow. Clear clusters and relationships between breeds that originated from the same region or area were detected. However, in spite the complex admixture history of most breeds, many of the local Italian cattle breeds have retained unique identities and are differentiated breeds, and this can be due to differences in the origin, differences in climate, genetic isolation and inbreeding. This study represents the first exhaustive analysis of Italian cattle diversity. The results largely agreed with the breeding history of the Italian cattle breeds. The population structure and the low genetic diversity presented here for several breeds should be useful in creating conservation strategies. If reproductive isolation within groups of the same herd and area is maintained in subsequent generations, the short-term rate of inbreeding will increase dangerously, resulting in a decrease in the effective population size and seriously damaging the breeds future. Thus, efforts should be made to improve genetic diversity in these breeds. In particular, mating decisions will play an important role in limiting inbreeding and will increase the size of these local breeds.

Analysis of genomic data is increasingly becoming part of the livestock industry and is an invaluable resource for effective management of breeding programs in small populations. The recent availability of genome-wide SNP panels allows providing background information concerning genome structure in domestic animals, opening new perspectives to livestock genetics. BOVITA was established to join local efforts and resources for the genomic characterization of Italian local cattle breeds. Despite the growing diffusion of some cosmopolite specialized breeds, several autochthonous breeds are still bred in Italy. The main aim of the BOVITA is to investigate the genomic structure of Italian local cattle breeds, to provide information on their genetic status that will be useful for the management of the genetic variability, as a contribution to biodiversity conservation and prioritization actions. A total of about 800 animals (20-32 per breed) belonging to thirty Italian cattle breeds (Agerolese, Barà-Pustertaler, Burlina, Cabannina, Calvana, Chianina, Cinisara, Garfagnina, Italian Brown, Italian Holstein, Italian Simmental, Marchigiana, Maremmana, Modenese, Modicana, Mucca Pisana, Pezzata Rossa d’Oropa, Piedmontese, Pinzgau, Podolica, Pontremolese, Pustertaler, Reggiana, Rendena, Romagnola, Rossa Siciliana, Sarda, Sardo-Bruna, Sardo-Modicana and Ottonese-Varzese) and two cosmopolitan breeds (Charolaise and Limousine) genotyped with the Illumina BovineSNP50 v2 BeadChip array were collected for the analysis. The genotypes of several breeds were detected in the frame of the project, whereas for some breeds these data are derived by previous studies. The dataset will be analyzed to: study several aspects of population genetic diversity, multi-dimensional scaling plot, population structure, linkage disequilibrium, and runs of homozygosity. In addition, comparative analysis of conserved haplotypes will be conducted to identify genomic segments under selection pressure. Such information also provides important insights into the mechanisms of evolution and is useful for the annotation of significant functional genomics regions. Data analysis will also be useful to select SNPs suitable for parentage test and breed genetic traceability. The analysis of the data will pinpoint the genetic distinctiveness of Italian breeds. Moreover, the obtained results contribute to a better characterization of history and genetic structure of Italian cattle breeds.