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21 results on '"Díaz-Anaya, Adriana María"'

4. Seroprevalence and risk factors associated with the presence of bovine leptospirosis in the municipality of Sotaquirá, Colombia

Author

Bulla-Castañeda, Diana María, Buitrago, Henry Alexander Lopez, Lancheros-Buitrago, Deisy Johana, Díaz-Anaya, Adriana María, Garcia-Corredor, Diego Jose, Tobón-Torreglosa, Julio Cesar, Ortega, Diego Ortiz, and Pulido-Medellín, Martín Orlando

Subjects
Cattle, Leptospira, Leptospirosis, Prevalence
Abstract

Background: Bovine leptospirosis is a zoonotic, infectious, and cosmopolitan disease of worldwide distribution, caused by the spirochete Leptospira spp., which has been diagnosed in humans; domestic mammals, such as dogs, sheep, goats, swine, horses and cattle; and wild animals. It is considered a significant cause of economic losses in livestock because it causes infertility, abortion and reduced milk production. Aim: To establish the prevalence and the main risk factors associated with Leptospira spp. in cattle in the municipality of Sotaquirá, Colombia. Methods: An observational, descriptive, cross-sectional study with simple random sampling was carried out. 1,000 cattle of Ayrshire, Holstein, Jersey, Normande, Zebu, and crossbreeds were sampled. Blood samples were taken by coccygeal venipuncture and processed by microscopic agglutination technique; animals were considered positive when titers were ≥1:100. The data obtained were processed with the statistical program EpiInfo®. Results: A general apparent prevalence (AP) of 16% (160/1,000) was established, where the crossbreeds (20.5% AP), the 2–4 years age group (17% AP), and the serovars Leptospira interrogans serogroup Pomona (5.1%) and L. interrogans serogroup Sjroe serovar Hardjo (3.4%) presented the highest seropositivity. The variables barnyard, artificial insemination, and use of certified semen were identified as protective factors against the disease, while diarrhea was considered a risk factor. Conclusion: The prevalence in this study is within the range of those reported at the national level; however, it is essential to establish plans to control and prevent the disease.

Published
2022

9. Determinación de anticuerpos antidiarrea viral bovina (DVB) en vacas lecheras de un municipio de Boyacá (Colombia)

Author

González-Bautista, Edgar Daniel, Bulla-Castañeda, Diana María, Díaz-Anaya, Adriana María, García-Corredor, Diego José, Pulido-Medellín, Martín Orlando, González-Bautista, Edgar Daniel, Bulla-Castañeda, Diana María, Díaz-Anaya, Adriana María, García-Corredor, Diego José, and Pulido-Medellín, Martín Orlando

Abstract

Bovine viral diarrhea (BVD) is an infectious pathology generated by a pestivirus of worldwide distribution, which causes reproductive problems and economic losses. The objective of this study was to establish bovine viral diarrhea virus (BVDV) positivity in cows from the municipality of Tuta (Boyacá, Colombia), and to analyze age and racial groups and reproductive and management variables as possible risk factors. A total of 374 blood samples were taken and the indirect ELISA test was performed using the Serelisa® BVD p80 Ab Mono Blocking kit; the data were processed with EpiInfo®. A seroprevalence of 41.7% was found. Crossbreds and cattle >4 years had the highest seroprevalence. Animals >4 years old (p= 0.0000001922) were statistically associated with the presence of the disease and were considered a risk factor for BVDV. Control and prevention programs should be established to hinder its dissemination in the area., Resumen La diarrea viral bovina (DVB) es una patología infecciosa generada por un pestivirus de distribución mundial, causante de problemas reproductivos y pérdidas económicas. El objetivo del presente estudio fue establecer la positividad al virus de diarrea viral bovina (vDVB) en vacas del municipio de Tuta (Boyacá, Colombia), y analizar los grupos etarios, raciales y las variables reproductivas y de manejo como posibles factores de riesgo. Se tomaron 374 muestras de sangre, a las cuales se les realizó la prueba ELISA indirecta, implementando el kit Serelisa® BVD p80 Ab Mono Blocking; los datos se procesaron con EpiInfo®. Se encontró una seroprevalencia del 41,7 %. Los cruces raciales y los bovinos >4 años presentaron la seroprevalencia más alta. Los animales >4 años (p= 0,0000001922) presentaron asociación estadística con la presencia de la enfermedad, y se consideró factor de riesgo para vDVB. Se deben establecer programas de control y prevención que dificulten su diseminación en la zona.

Published
2021

11. Serodiagnóstico de Paratuberculosis en bovinos del municipio de Sogamoso, Boyacá (Colombia)

Author

Bulla Castañeda, Diana María, Díaz Anaya, Adriana María, García Corredor, Diego José, Pulido Medellín, Martín Orlando, Bulla Castañeda, Diana María, Díaz Anaya, Adriana María, García Corredor, Diego José, and Pulido Medellín, Martín Orlando

Abstract

Bovine Paratuberculosis (BPT), or Johne's Disease, is an infection of the gastrointestinal tract caused by Mycobacterium avium, subspecies paratuberculosis (Map), which is characterized by chronic granulomatous enteritis and lymphadenitis in ruminants. The most relevant concern regarding the importance of BPT is its possible link to Crohn's disease (CD) in humans, however this association is still under investigation. The seroprevalence of BPT was determined in the municipality of Sogamoso (Boyacá), where 604 blood samples were collected, their serum was processed by the indirect ELISA technique with the commercial PARACHEK® 2 KIT (Prionics, Switzerland), following the manufacturer's instructions. The seroprevalence was 10,9% (66/604), with the 2 to 3 years age group and the Jersey breed having the highest seroprevalence. A significant statistical association was found (p≤0,05) with the age of the individuals tested and the supply of concentrate. The seroprevalence found indicates that active transmission of the disease is taking place, and that the available control measures are not being carried out or are not effective enough., A Paratuberculose Bovina (PTB) ou Doença de Johne é uma infecção do trato gastrointestinal causada pela Mycobacterium avium subespécie paratuberculosis (Map), caracterizada por gerar enterite granulomatosa crónica e linfadenite em ruminantes. A preocupação mais relevante em relação à importância do PTB é seu possível vínculo com a Doença de Crohn (DC) em seres humanos, no entanto, essa associação ainda está sob investigação. A soroprevalência do PTB foi determinada no município de Sogamoso (Boyacá), onde foram coletadas 604 amostras de sangue, cujo soro foi processado pela técnica ELISA indireta com o kit comercial PARACHEK® 2 KIT (Prionics, Suíça). A soroprevalência foi de 10,9% (66/604), onde a faixa etária de 2 a 3 anos e a raça Jersey foram as que apresentaram maior soroprevalência. Foi encontrada associação estatisticamente significante (p≤0,05) com a idade dos indivíduos avaliados e o suprimento de concentrado. A soroprevalência encontrada indica que a transmissão ativa da doença está ocorrendo e que as medidas de controle disponíveis não estão sendo realizadas ou não são eficazes o suficiente., La Paratuberculosis Bovina (PTB) o Enfermedad de Johne, es una infección del tracto gastrointestinal causada por Mycobacterium avium, subespecie paratuberculosis (Map), que se caracteriza por generar enteritis granulomatosa crónica y linfadenitis en rumiantes. La preocupación más relevante en relación con la importancia de la PTB es su posible vínculo con la Enfermedad de Crohn (EC) en humanos, sin embargo, esta asociación aún está bajo investigación. Se determinó la seroprevalencia de PTB en el municipio de Sogamoso (Boyacá), donde se recolectaron 604 muestras de sangre, cuyo suero fue procesado mediante la técnica de ELISA indirecta con el kit comercial PARACHEK® 2 KIT (Prionics, Suiza). La seroprevalencia fue de 10,9% (66/604), donde el grupo etario de 2 a 3 años y la raza Jersey fueron los de mayor seroprevalencia. Se encontró asociación estadística significativa (p≤0,05) entre la edad de los individuos evaluados y el suministro de concentrado. La seroprevalencia encontrada indica que se está produciendo una transmisión activa de la enfermedad y que las medidas de control disponibles no están siendo llevadas a cabo o no son lo suficientemente efectivas.

Published
2020

12. Epidemiología molecular de las enfermedades parasitarias del tracto respiratorio en pequeños rumiantes del departamento de Boyacá

Author

Díaz Anaya, Adriana María, Vargas Duarte, Jimmy Jolman (Director de tesis), and Pulido Medellín, Martín Orlando (Codirector de tesis)

Subjects
Caprinos, Maestría en Ciencias Biológicas - Tesis y disertaciones académicas, Epidemiología veterinaria, Parasitología veterinaria, Epidemiología molecular, Agrosavia, Ovinos
Abstract

1 recurso en línea (93 páginas) : ilustraciones color, figuras, tablas., El objetivo del presente trabajo es realizar una aproximación a la epidemiología molecular de las enfermedades parasitarias presentes en el tracto respiratorio de ovinos y caprinos de la provincia Norte del departamento de Boyacá., Bibliografía y webgrafía: páginas 38-46, Maestría, Magíster en Ciencias Biológicas

Published
2019

14. Prevalencia de Fasciola hepatica en Bovinos Sacrificados en la Planta de Beneficio del Municipio de Une, Cundinamarca, Colombia

Author

Giraldo Forero, Julio Cesar, Díaz Anaya, Adriana María, and Pulido Medellín, Martín Orlando

Subjects
trematode, estudio transversal, bilis, cattle, trematodo, bovinos, cross-sectional study, bile, Fasciola hepatica
Abstract

The aim if this study was to determine the prevalence of Fasciola hepatica in cattle at the slaughterhouse of the municipality of Une, Cundinamarca, Colombia. Sample size was calculated based on the slaughter rate of 2013 plus 6% increase (n=142). Adult parasites were collected from the biliary duct. Bile and faecal material were also collected. The modified Ritchie-Frick technique was used to analyse faecal samples. The prevalence of F. hepatica was 39.4% based on eggs in the bile, 32.4% based on the presence of adult parasites in biliary ducts and 15.5% based on the presence of eggs in faecal samples. Positive animals for presence of adult parasites plus eggs in bile and faecal samples was 15.5%. El objetivo del presente estudio fue determinar la prevalencia de Fasciola hepatica en bovinos sacrificados en la planta de beneficio del municipio de Une, Cundinamarca, Colombia. El tamaño muestral fue calculado con base a la tasa de sacrificio de 2013 con 6% de incremento (n=142). Durante el eviscerado se recolectaron distomas adultos del ducto biliar, así como muestras del contenido biliar y materia fecal. En el examen coproparasitológico se empleó la técnica de Ritchie-Frick modificada. La prevalencia de F. hepatica fue 39.4% por presencia de huevos en el contenido biliar, 32.4% por presencia de parásitos adultos en los ductos biliares y 15.5% por presencia de huevos en materia fecal. El valor porcentual de animales positivos por presencia de estadio adulto, así como huevos en bilis y materia fecal fue de 15.5%.

Published
2017

18. Estudio coproparasitológico en ovinos al pastoreo en Boyacá, Colombia.

Author

Díaz-Anaya, Adriana María, Chavarro-Tulcán, Ginette Isabel, Pulido Medellín, Martín Orlando, García-Corredor, Diego, and Vargas-Avella, Julio Cesar

Subjects
*SHEEP parasites, *GASTROINTESTINAL diseases, *MICROBIOLOGY, *FECES
Abstract

The purpose of this study was to know the mainly gastrointestinal parasites affecting sheep and their prevalence in Boyacá. Six hundred and thirty seven fecal samples of grazing sheep were collected. It was found that 89.4 % of sheep were parasitized. The samples were processed by the modified Ritchie technique and visualized in optical microscopy. The Eimeriidae family had the highest prevalence (63 %), followed by Trichostrongylidae (47.4 %), Dyctiocaulidae (38.1 %) and Strongyloidae with a prevalence of 21.5 %. The families Fasciolidae (6.3 %), Trichuridae (5.7 %), Anoplocefalidae (2.4 %), Toxocaridae (1.3 %), Taeniidae (0.3 %), and Capillaridae (0.2 %) had a lower prevalence. These results are consistent with the prevailing climatic conditions in the production areas, where egg and larvae survival values have decreased. It is important to implement health programs, provide adequate food for animals, reduce stress factors and establish regular deworming programs for the control of these parasite populations in such region. [ABSTRACT FROM AUTHOR]

Published
2017

20. Determinación de anticuerpos anti Neospora caninum en vacas de la provincia de Sugamuxi, Colombia.

Author

Pulido Medellín, Martín Orlando, Díaz Anaya, Adriana María, García, Diego José, and Andrade Becerra, Roy José

Subjects
*NEOSPORA caninum, *IMMUNOGLOBULINS, *COWS, *DAIRY farms
Abstract

The purpose of the present study was establishing the presence of anti Neospora caninum antibodies in cows in the province of Sugamuxi, Colombia, through indirect ELISA and identifying variables which influence susceptibility of bovines to this protozoan. Blood samples from 400 females were evaluated through indirect ELISA test. A 57.5 % Neospora caninum seroprevalence was found. No relationship between pregnancy and presence of parasite was found. Moreover, 27.7 % of infested cows repeated estrus. A 100 % presence of this parasite was determined in females with a history of five parturitions. Results of this study confirm presence of anti Neospora caninum antibodies in dairy farm animals in the province of Sugamuxi, and this points out potential risks for this type of farms. [ABSTRACT FROM AUTHOR]

Published
2013

21. [Nematodes with zoonotic potential in parks of the city of Tunja, Colombia].

Author

Díaz-Anaya AM, Pulido-Medellín MO, and Giraldo-Forero JC

Subjects
Animals, Cities, Colombia, Dog Diseases epidemiology, Dogs parasitology, Helminthiasis, Animal epidemiology, Humans, Intestinal Diseases, Parasitic epidemiology, Intestinal Diseases, Parasitic veterinary, Larva, Nematoda growth & development, Ovum, Disease Reservoirs, Feces parasitology, Nematoda isolation & purification, Parks, Recreational statistics & numerical data, Soil parasitology, Urban Health, Zoonoses transmission
Abstract

Objective: To identify the presence of parasites with zoonotic potential in major parks in the city of Tunja, Boyacá., Materials and Methods: Twenty eight parks in the city were selected, where 124 samples of feces of dogs and soil were collected with the help of a spatula, gathering approximately 150 g per sample. They were processed by the method of concentration of Ritchie modified making the identification of parasitic forms in an optical microscope., Results: A 60.7% of the parks were positive to nematodes in samples of canine fecal material and 100% on soil. Found nematodes were eggs and larvae of Toxocara spp., Ancylostoma spp., Trichuris vulpis and Strongiloides spp., Conclusion: This study demonstrated the potential risk of transmission of zoonoses caused by nematodes in canines and for the need to strengthen public health measures to reduce the risk shows the population exposed to such zoonoses.

Published
2015

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