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196 results on '"Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA )"'

1. Polymers and nano-objects, a rational combination for developing health monitoring biosensors

Author: Cosnier, Serge, Holzinger, Michael, Paulo, Henrique, Buzzetti, Serge, Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA ), Département de Chimie Moléculaire (DCM), Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), Université Joseph Fourier - Grenoble 1 (UJF)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Cosnier, Serge
Subjects: [CHIM.MATE] Chemical Sciences/Material chemistry, [CHIM.ANAL] Chemical Sciences/Analytical chemistry, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, [CHIM.MATE]Chemical Sciences/Material chemistry
Abstract: International audience; Biosensors are essential tools in the health and the environmental sectors since they allow fast and reliable diagnostics and analysis. Biosensors are defined by the biological sensing element, which contains biomolecules or synthetic, bioinspired entities with unique specificity towards the analyte. The biological or bioinspired recognition even can be transduced into an electric, electrochemical, or optical signal. A constant challenge for the development of biosensors is the confinement of these selective entities in devices without altering the biological activity. Polymers are great candidates to serve as substrates for the immobilization or entrapment of bioreceptors related to well established synthesis routes with highly modulable chemical functions to create an almost ideal environment for the biomolecules. Nanomaterials are of constant increasing interest since they allow not only to increase drastically the specific surface for higher amounts of receptor units, but also provide electric or optical properties leading to enhanced signal capture. As for bioreceptors, such nanomaterials can be integrated polymer matrices for reliable processability. This review aims to summarize selected original examples about biosensors for health monitoring using beneficial combinations of nanomaterials and polymers.
Published: 2021

2. ATMP derived cobalt-metaphosphate complex as highly active catalyst for oxygen reduction reaction

Author: Dan Shan, Serge Cosnier, Shengli Zhang, Lian-Hua Xu, Haibo Zeng, Xueji Zhang, Shiying Guo, Wenju Wang, Robert S. Marks, Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA ), Département de Chimie Moléculaire (DCM), Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Ben-Gurion University of the Negev (BGU), and Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 010405 organic chemistry, Coordination polymer, Metaphosphate, Inorganic chemistry, chemistry.chemical_element, 010402 general chemistry, Electrocatalyst, 01 natural sciences, Catalysis, 0104 chemical sciences, Active center, chemistry.chemical_compound, chemistry, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Methanol, Physical and Theoretical Chemistry, ATMP, Cobalt, ComputingMilieux_MISCELLANEOUS
Abstract: Rational design and facile synthesis of highly active electrocatalysts with low cost for oxygen reduction reaction (ORR) are always of great challenge. Specifically, development of a new type of energy-saving materials with convenient method is regarded as the current bottleneck. Herein, an innovative strategy based on amino trimethylene phosphonic acid (ATMP) as chelating agent for cobalt-metaphosphate coordination polymer is reported to one-pot synthesis of a novel precursor in methanol for ORR electrocatalyst. Carbonization of the precursor at 900 °C at N2 atmosphere results in the feasible formation of cobalt metaphosphate based composite (Co(PO3)2/NC). A further step in the thermal cleavage at 650 °C at air for 4 h, Co(PO3)2/NC can be finally transformed into inorganic Co(PO3)2. Advanced spectroscopic techniques and density function theory (DFT) calculations are applied to confirm the main catalytically active center and the physical properties of Co(PO3)2/NC. This obtained Co(PO3)2/NC nanocomposite exhibits superior electrocatalysis to Co(PO3)2 with an enhanced onset potential (0.906 V vs. RHE) and diffusion limiting current (5.062 mA cm−2), which are roughly close to those of commercial 20% Pt/C (0.916 V, 5.200 mA cm−2).
Published: 2020
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3. Polymers and nano-objects, a rational combination for developing health monitoring biosensors

Author: Holzinger, Michael, Buzzetti, Paulo Henrique, Cosnier, Serge, Département de Chimie Moléculaire (DCM), Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA ), Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Holzinger, Michael
Subjects: [CHIM.ANAL] Chemical Sciences/Analytical chemistry, [CHIM.ANAL]Chemical Sciences/Analytical chemistry
Abstract: International audience; Biosensors are essential tools in the health and the environmental sectors since they allow fast and reliable diagnostics and analysis. Biosensors are defined by the biological sensing element, which contains biomolecules or synthetic, bioinspired entities with unique specificity towards the analyte. The biological or bioinspired recognition even can be transduced into an electric, electrochemical, or optical signal. A constant challenge for the development of biosensors is the confinement of these selective entities in devices without altering the biological activity. Polymers are great candidates to serve as substrates for the immobilization or entrapment of bioreceptors related to well established synthesis routes with highly modulable chemical functions to create an almost ideal environment for the biomolecules. Nanomaterials are of constant increasing interest since they allow not only to increase drastically the specific surface for higher amounts of receptor units, but also provide electric or optical properties leading to enhanced signal capture. As for bioreceptors, such nanomaterials can be integrated polymer matrices for reliable processability. This review aims to summarize selected original examples about biosensors for health monitoring using beneficial combinations of nanomaterials and polymers.
Published: 2021

4. Insights into carbon nanotube-assisted electro-oxidation of polycyclic aromatic hydrocarbons for mediated bioelectrocatalysis

Author: Alan Le Goff, Emerson M. Girotto, Pierre Yves Blanchard, Yuta Nishina, Serge Cosnier, Michael Holzinger, Paulo Henrique M. Buzzetti, Département de Chimie Moléculaire (DCM), Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), State University of Maringá, Research Core for Interdisciplinary Sciences [Okayama] (RCIS), Okayama University, Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA ), and Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: genetic structures, Glucose Dehydrogenases, 02 engineering and technology, Carbon nanotube, 010402 general chemistry, Photochemistry, 01 natural sciences, Catalysis, law.invention, Fungal Proteins, chemistry.chemical_compound, Electron transfer, Glucose dehydrogenase, law, Materials Chemistry, Polycyclic Aromatic Hydrocarbons, ComputingMilieux_MISCELLANEOUS, health care economics and organizations, Naphthalene, Nanotubes, Carbon, Metals and Alloys, Quinones, [CHIM.CATA]Chemical Sciences/Catalysis, General Chemistry, Electrochemical Techniques, Phenanthrene, 021001 nanoscience & nanotechnology, eye diseases, Coronene, 0104 chemical sciences, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Aspergillus, Glucose, chemistry, Ceramics and Composites, Biocatalysis, Flavin-Adenine Dinucleotide, Pyrene, sense organs, 0210 nano-technology, Oxidation-Reduction, Perylene
Abstract: A series of polycyclic aromatics, naphthalene, phenanthrene, perylene, pyrene, 1-pyrenebutyric acid N-hydroxysuccinimide ester (pyrene NHS) and coronene, were immobilized via π stacking on carbon nanotube (CNT) electrodes and electro-oxidized in aqueous solutions. The obtained quinones were characterized and evaluated for the mediated electron transfer with FAD dependent glucose dehydrogenase during catalytic glucose oxidation.
Published: 2021
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5. Monofunctional pyrenes at carbon nanotube electrodes for direct electron transfer H2O2 reduction with HRP and HRP-bacterial nanocellulose

Author: Sara Bocanegra-Rodríguez, Pilar Campíns-Falcó, Carmen Molins-Legua, Fabien Giroud, Serge Cosnier, Andrew J. Gross, Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA ), Département de Chimie Moléculaire (DCM), Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Biomedical Engineering, Biophysics, 02 engineering and technology, Carbon nanotube, 01 natural sciences, 7. Clean energy, Nanocellulose, law.invention, Catalysis, Biofuel cell cathode, Horseradish peroxidase, chemistry.chemical_compound, Electron transfer, law, Electrochemistry, [CHIM]Chemical Sciences, ComputingMilieux_MISCELLANEOUS, Chemistry, 010401 analytical chemistry, General Medicine, Nanocellulose electrode, 021001 nanoscience & nanotechnology, Combinatorial chemistry, 0104 chemical sciences, Electrochemical gas sensor, Electrochemical sensor, Direct electron transfer, Electrode, Bioelectrocatalysis, Pyrene, 0210 nano-technology, Biosensor, Biotechnology
Abstract: The non-covalent modification of carbon nanotube electrodes with pyrene derivatives is a versatile approach to enhance the electrical wiring of enzymes for biosensors and biofuel cells. We report here a comparative study of five pyrene derivatives adsorbed at multi-walled carbon nanotube electrodes to shed light on their ability to promote direct electron transfer with horseradish peroxidase (HRP) for H2O2 reduction. In all cases, pyrene-modified electrodes enhanced catalytic reduction compared to the unmodified electrodes. The pyrene N-hydroxysuccinimide (NHS) ester derivative provided access to the highest catalytic current of 1.4 mA cm−2 at 6 mmol L−1 H2O2, high onset potential of 0.61 V vs. Ag/AgCl, insensitivity to parasitic H2O2 oxidation, and a large linear dynamic range that benefits from insensitivity to HRP “suicide inactivation” at 4–6 mmol L−1 H2O2. Pyrene-aliphatic carboxylic acid groups offer better sensor sensitivity and higher catalytic currents at ≤ 1 mmol L−1 H2O2 concentrations. The butyric acid and NHS ester derivatives gave high analytical sensitivities of 5.63 A M−1 cm−2 and 2.96 A M−1 cm−2, respectively, over a wide range (0.25–4 mmol−1) compared to existing carbon-based HRP biosensor electrodes. A bacterial nanocellulose pyrene-NHS HRP bioelectrode was subsequently elaborated via “one-pot” and “layer-by-layer” strategies. The optimised bioelectrode exhibited slightly weaker voltage output, further enhanced catalytic currents, and a major enhancement in 1-week stability with 67% activity remaining compared to 39% at the equivalent electrode without nanocellulose, thus offering excellent prospects for biosensing and biofuel cell applications.
Published: 2021
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6. Postmodulation of the Metal–Organic Framework Precursor toward the Vacancy-Rich Cu x O Transducer for Sensitivity Boost: Synthesis, Catalysis, and H 2 O 2 Sensing

Author: Robert S. Marks, Junji Li, Serge Cosnier, Xueji Zhang, Guofang Shu, Dan Shan, Wen-Li Xin, Yu-Xuan Dai, Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA ), Département de Chimie Moléculaire (DCM), and Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Nanocomposite, Dopant, Chemistry, 02 engineering and technology, [CHIM.CATA]Chemical Sciences/Catalysis, 010402 general chemistry, 021001 nanoscience & nanotechnology, Electrochemistry, 01 natural sciences, 0104 chemical sciences, Analytical Chemistry, Catalysis, Transducer, Chemical engineering, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Vacancy defect, Atom, Metal-organic framework, 0210 nano-technology, ComputingMilieux_MISCELLANEOUS
Abstract: Metal-organic frameworks (MOFs) act as versatile coordinators for the subsequent synthesis of high-performance catalysts by providing dispersed metal-ion distribution, initial coordination condition, dopant atom ratios, and so on. In this work, a crystalline MOF trans-[Cu(NO3)2(Him)4] was synthesized as the novel precursor of a redox-alternating CuxO electrochemical catalyst. Through simple temperature modulation, the gradual transformation toward a highly active nanocomposite was characterized to ascertain the signal enhancing mechanism in H2O2 reduction. Owing to the proprietary structure of the transducer material and its ensuing high activity, a proof-of-principle sensor was able to provide an amplified sensitivity of 2330 μA mM-1 cm-2. The facile one-pot preparation and intrinsic nonenzymatic nature also suggests its wide potentials in medical settings.
Published: 2021
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7. Electrosynthesis of Pyrenediones on Carbon Nanotube Electrodes for Efficient Electron Transfer with FAD‐dependent Glucose Dehydrogenase in Biofuel Cell Anodes

Author: Emerson M. Girotto, Michael Holzinger, Yuta Nishina, Yannig Nedellec, Bridget Davies, Pierre Yves Blanchard, Paulo Henrique M. Buzzetti, Alan Le Goff, Andrew J. Gross, Serge Cosnier, Département de Chimie Moléculaire (DCM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Universidade Estadual de Maringá [Maringá] (UEM), Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), and Okayama University
Subjects: Chemistry, Cell, 02 engineering and technology, Carbon nanotube, 010402 general chemistry, 021001 nanoscience & nanotechnology, Electrosynthesis, 7. Clean energy, 01 natural sciences, Catalysis, 0104 chemical sciences, law.invention, Anode, Electron transfer, medicine.anatomical_structure, Chemical engineering, law, Biofuel, Glucose dehydrogenase, Electrode, Electrochemistry, medicine, [CHIM.OTHE]Chemical Sciences/Other, 0210 nano-technology
Abstract: International audience; A particularly efficient redox mediator for electron transfer between FAD‐dependent glucose dehydrogenase (FAD‐GDH) and carbon nanotube (CNT) based electrodes can be obtained via electrosynthetic oxidation of pyrene in aqueous buffer solution. 1H‐NMR spectroscopic studies reveal the formation of a 2 : 1 mixture of 1,6‐pyrenedione and 1,8‐pyrenedione at the electrode. The formed pyrenedione exhibits a well‐defined surface‐bound redox system at −0.1 V vs. SCE and provides excellent electron transfer kinetics with this enzyme. Furthermore, the π‐system of pyrenedione allows improved stacking behavior with the CNT walls, leading to enhanced stabilities compared to commonly used mediators like naphthoquinone. The electrosynthesis of pyrenedione for catalytic glucose oxidation is optimal at pH 2 using cyclic voltammetry or chronoamerometry. It is envisioned that the electrosynthetic methodology can be expanded to form different redox mediators for a series of enzymes.
Published: 2019
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8. Mononuclear Ni(II) Complexes with a S3O Coordination Sphere Based on a Tripodal Cysteine-Rich Ligand: pH Tuning of the Superoxide Dismutase Activity

Author: Christelle Gateau, Pascale Maldivi, Pascale Delangle, Olivier Proux, Jérémy Domergue, Alan Le Goff, Jacques Pécaut, Carole Duboc, Colette Lebrun, Chimie Interface Biologie pour l’Environnement, la Santé et la Toxicologie (CIBEST), SYstèmes Moléculaires et nanoMatériaux pour l’Energie et la Santé (SYMMES), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Département de Chimie Moléculaire - Chimie Inorganique Redox Biomimétique (DCM - CIRE), Université Joseph Fourier - Grenoble 1 (UJF)-Institut de Chimie Moléculaire de Grenoble (ICMG)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Observatoire des Sciences de l'Univers de Grenoble (OSUG), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), Département de Chimie Moléculaire (DCM), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Conception d’Architectures Moléculaires et Processus Electroniques (CAMPE), Chimie Interface Biologie pour l’Environnement, la Santé et la Toxicologie (CIBEST ), Institut de Chimie du CNRS (INC)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Département de Chimie Moléculaire - Chimie Inorganique Redox Biomimétique (DCM - CIRE ), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Département Interfaces pour l'énergie, la Santé et l'Environnement (DIESE), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Observatoire des Sciences de l'Univers de Grenoble (OSUG ), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national des sciences de l'Univers (INSU - CNRS)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Département de Chimie Moléculaire - Chimie Inorganique Redox (DCM - CIRE ), and ANR-17-EURE-0003,CBH-EUR-GS,CBH-EUR-GS(2017)
Subjects: Coordination sphere, Stereochemistry, Superoxide dismutase activity, Ligands, 010402 general chemistry, 01 natural sciences, Inorganic Chemistry, Superoxide dismutase, Biomimetic Materials, Coordination Complexes, Nickel, [CHIM]Chemical Sciences, Cysteine, Physical and Theoretical Chemistry, ComputingMilieux_MISCELLANEOUS, Sulfur Compounds, biology, Superoxide Dismutase, 010405 organic chemistry, Ligand, Chemistry, Hydrogen-Ion Concentration, 3. Good health, 0104 chemical sciences, biology.protein, Oxidation-Reduction
Abstract: The superoxide dismutase (SOD) activity of mononuclear NiII complexes, whose structures are inspired by the NiSOD, has been investigated. They have been designed with a sulfur-rich pseudopeptide li...
Published: 2019
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9. Efficient Electrochemical CO2/CO Interconversion by an Engineered Carbon Monoxide Dehydrogenase on a Gas-Diffusion Carbon Nanotube-Based Bioelectrode

Author: Julien Pérard, Clara Rinaldi, Christine Cavazza, Bruno Guigliarelli, Umberto Contaldo, Alan Le Goff, BioEnergie et Environnement (BEE), Laboratoire de Chimie et Biologie des Métaux (LCBM - UMR 5249), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA ), Département de Chimie Moléculaire (DCM), Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Bioénergétique et Ingénierie des Protéines (BIP ), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), ANR-11-LABX-0003,ARCANE,Grenoble, une chimie bio-motivée(2011), ANR-17-EURE-0003,CBH-EUR-GS,CBH-EUR-GS(2017), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), and Département de Chimie Moléculaire - Bioélectrochimie pour les capteurs, l’énergie et les nanomatériaux (DCM - BioCEN)
Subjects: medicine.medical_specialty, bioelectrocatalysis, Inorganic chemistry, carbon monoxide dehydrogenase, Overpotential, 010402 general chemistry, Electrochemistry, 01 natural sciences, Catalysis, medicine, [PHYS]Physics [physics], biology, carbon nanotubes, 010405 organic chemistry, Chemistry, Rhodospirillum rubrum, Active site, General Chemistry, [CHIM.CATA]Chemical Sciences/Catalysis, [CHIM.MATE]Chemical Sciences/Material chemistry, biology.organism_classification, 0104 chemical sciences, Turnover number, Bioelectrochemistry, biology.protein, nickel enzymes, EPR, Carbon monoxide dehydrogenase, CO2 RR
Abstract: International audience; Carbon monoxide dehydrogenase catalyzes the reversible oxidation of CO to CO2. The monofunctional enzyme from Rhodospirillum rubrum (RrCODH) has been extensively characterized in the past, although its use and investigation by bioelectrochemistry have been limited. Here, we developed a heterologous system yielding a highly stable and active recombinant RrCODH in one-step purification, with CO oxidation activity reaching a maximum of 26 500 U.mg(-1), making RrCODH the most active CODH under ambient conditions described so far. Electron paramagnetic resonance was used to precisely characterize the recombinant RrCODH, demonstrating the integrity of the active site. Selective CO2/CO interconversion with maximum turnover frequencies of 150 s(-1) for CO oxidation (1.5 mA cm(-2) at 250 mV overpotential) and 420 s(-1) for CO2 reduction (4.2 mA cm(-2) at 180 mV overpotential) is catalyzed by the recombinant RrCODH immobilized on MWCNT electrodes modified with 1-pyrenebutyric acid adamantyl amide (MWCNTADA), either in a classic three-electrode cell or in specifically designed CO2/CO-diffusing electrodes. This functional device is stable for hours with a turnover number of at least 800 000. The performances of recombinant RrCODH-modified MWCNTADA are close to the best metal-based and molecular-based catalysts. These results greatly increase the benchmark for bioelectrocatalysis of reversible CO2 conversion.
Published: 2021
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10. Microcapsule-based biosensor containing catechol for the reagent-free inhibitive detection of benzoic acid by tyrosinase

Author: Pierre Agostini, Omar Elmazria, Serge Cosnier, Karine Gorgy, Chantal Gondran, Stefan Mc Murtry, Yannig Nedellec, Département de Chimie Moléculaire (DCM), Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut Jean Lamour (IJL), Université de Lorraine (UL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA ), and Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Tyrosinase, Biomedical Engineering, Biophysics, Enzyme electrode, Catechols, Capsules, 02 engineering and technology, Biosensing Techniques, 01 natural sciences, chemistry.chemical_compound, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Electrochemistry, ComputingMilieux_MISCELLANEOUS, Benzoic acid, Detection limit, Catechol, Monophenol Monooxygenase, Nanotubes, Carbon, 010401 analytical chemistry, Substrate (chemistry), General Medicine, Benzoic Acid, 021001 nanoscience & nanotechnology, Enzymes, Immobilized, 0104 chemical sciences, chemistry, Indicators and Reagents, 0210 nano-technology, Biosensor, Methylene blue, Biotechnology, Nuclear chemistry
Abstract: A biosensor based on the release of the enzyme substrate from its structure was developed for the inhibitive detection of benzoic acid. A polyurethane support comprising two perforated microcapsules (800 μm in diameter) filled with methylene blue as a model compound and covered with a conductive deposit of multiwalled carbon nanotubes, continuously released this stored dye for 24 h. An increase in methylene blue concentration of 0.5–0.75 μmol L-1 h-1 and 1.5–2 μmol L-1 h-1, in the presence and absence of the multiwalled carbon nanotube coating, respectively, was demonstrated by UV–vis spectroscopy in a 2 mL UV cuvette. The same configuration with microcapsules filled with catechol was modified by a laponite clay coating containing tyrosinase enzyme. The resulting biosensor exhibits a constant cathodic current at −0.155 V vs AgCl/Ag, due to the reduction of the ortho-quinone produced enzymatically from the released catechol. The detection of benzoic acid was recorded from the decrease in cathodic current due to its inhibiting action on the tyrosinase activity. Reagentless biosensors based on different deposited quantity of tyrosinase (100, 200, 400 and 600 μg) were investigated for the detection of catechol and applied to the detection of benzoic acid as inhibitor. The best performance was obtained with the 400 μg-based configuration, namely a detection limit of 0.4 μmol L-1 and a sensitivity of 228 mA L mol−1. After the inhibition process, the biosensors recover 97–100% of their activity towards catechol, confirming a reversible inhibition by benzoic acid.
Published: 2021
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11. Functionalizable Glyconanoparticles for a Versatile Redox Platform

Author: Christophe Travelet, Paulo Henrique M. Buzzetti, Muhammad Mumtaz, Serge Cosnier, Karine Gorgy, Marie Carrière, Redouane Borsali, Département de Chimie Moléculaire (DCM), Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherches sur les Macromolécules Végétales (CERMAV), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA ), and Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: General Chemical Engineering, block copolymer, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Redox, host-guest interaction, Dynamic light scattering, glyconanoparticles, Copolymer, General Materials Science, QD1-999, chemistry.chemical_classification, Cyclodextrin, Chemistry, anthraquinone sulfonate, Communication, Anthraquinone sulfonate, Spherical morphology, host–guest interaction, 021001 nanoscience & nanotechnology, maltoheptaose, 0104 chemical sciences, [CHIM.POLY]Chemical Sciences/Polymers, Chemical engineering, Transmission electron microscopy, β-cyclodextrin, Cyclic voltammetry, 0210 nano-technology, Functionalizable Glyconanoparticles
Abstract: International audience; A series of new glyconanoparticles (GNPs) was obtained by self-assembly by direct nanoprecipitation of a mixture of two carbohydrate amphiphilic copolymers consisting of polystyrene-block-β-cyclodextrin and polystyrene-block-maltoheptaose with different mass ratios, respectively 0–100, 10–90, 50–50 and 0–100%. Characterizations for all these GNPs were achieved using dynamic light scattering, scanning and transmission electron microscopy techniques, highlighting their spherical morphology and their nanometric size (diameter range 20–40 nm). In addition, by using the inclusion properties of cyclodextrin, these glyconanoparticles were successfully post-functionalized using a water-soluble redox compound, such as anthraquinone sulfonate (AQS) and characterized by cyclic voltammetry. The resulting glyconanoparticles exhibit the classical electroactivity of free AQS in solution. The amount of AQS immobilized by host–guest interactions is proportional to the percentage of polystyrene-block-β-cyclodextrin entering into the composition of GNPs. The modulation of the surface density of the β-cyclodextrin at the shell of the GNPs may constitute an attractive way for the elaboration of different electroactive GNPs and even GNPs modified by biotinylated proteins
Published: 2021
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12. Electrochemical modification at multiwalled carbon nanotube electrodes with Azure A for FADglucose dehydrogenase wiring: Structural optimization to enhance catalytic activity and stability

Author: Seiya Tsujimura, Andrew J. Gross, Michael Holzinger, Shunya Tanaka, Université de Tsukuba = University of Tsukuba, Département de Chimie Moléculaire (DCM), Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Holzinger, Michael, Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), Université Joseph Fourier - Grenoble 1 (UJF)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and ANR-15-JTIC-0002,Mocca-Cell,Design moléculaire d'architectures 3D biocatalytiques à base de nanostructures de carbone pour les piles à glucose de longue durée(2015)
Subjects: Nanotube, bioelectrocatalysis, Materials Science (miscellaneous), Azure A, Phenothiazine, 02 engineering and technology, 010402 general chemistry, Electrochemistry, glucose dehydrogenase electrode, 01 natural sciences, Catalysis, diazonium electrochemical grafting, chemistry.chemical_compound, Electron transfer, Glucose dehydrogenase, Materials Chemistry, [CHIM]Chemical Sciences, ComputingMilieux_MISCELLANEOUS, bioanode, [CHIM.CATA] Chemical Sciences/Catalysis, mediated electron transfer, Buffer solution, [CHIM.CATA]Chemical Sciences/Catalysis, 021001 nanoscience & nanotechnology, 0104 chemical sciences, General Energy, chemistry, Chemical engineering, FADGDH, Electrode, 0210 nano-technology, multiwalled carbon nanotube
Abstract: Electrochemical grafting is a suitable technology for fabricating electrode surfaces with new chemical functionalities whilst maintaining the bulk properties of the electrode, and electrochemical amine oxidation and diazonium salt reduction are two widely used techniques to achieve this end. Herein, we report the electrochemical reductive grafting of Azure A onto multiwalled carbon nanotube (MWCNT) electrodes for the efficient wiring of flavin adenine dinucleotide (FAD) dependent glucose dehydrogenase. The diazonium salt of Azure A is formed in situ and subsequently grafted onto the electrode surface through electrochemical reduction. The formal potential of the resultant Azure-A-modified electrode shifted to −0.05 V vs. Ag/AgCl upon radical coupling to the MWCNT electrode. Electron transfer from FAD buried in the protein shell to the electrode via Azure A was then observed in the presence of glucose in the buffer solution. This study focused on the important effect of CNT mass loading on Azure-A loading as well as bioelectrocatalytic activity and storage stability. The three-dimensional porous structure of the MWCNT electrode was determined to be favorable for the immobilization of flavin adenine dinucleotide dependent glucose dehydrogenase and efficient electron transfer via the Azure-A functionalities. The optimized 300 µg CNT-loaded modified electrode on glassy carbon (3 mm diameter) retains its initial activity for 3 d and 25% of its initial activity after 10 d. Furthermore, we show that grafted Azure A is stably immobilized on the MWCNTs for 1 month; therefore, the limiting stability factor is enzyme leaching and/or deactivation.
Published: 2021
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13. Freestanding biopellet electrodes based on carbon nanotubes and protein compression for direct and mediated bioelectrocatalysis

Author: Anastasiia Berezovska, Fabien Giroud, Serge Cosnier, Yannig Nedellec, Andrew J. Gross, Département de Chimie Moléculaire (DCM), Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA ), and Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Horseradish peroxidase, lcsh:Chemistry, Glucose dehydrogenase, Diaphorase, Electrochemistry, Glucose oxidase, isoelectric point, Bilirubin oxidase, biology, Chemistry, Cytochrome c, biofuel cells, [CHIM.MATE]Chemical Sciences/Material chemistry, 021001 nanoscience & nanotechnology, biosensors, 0104 chemical sciences, Isoelectric point, lcsh:Industrial electrochemistry, lcsh:QD1-999, disk electrodes, Galactose oxidase, biology.protein, bioelectrodes, 0210 nano-technology, enzyme wiring, lcsh:TP250-261, Nuclear chemistry
Abstract: International audience; The fabrication of bioelectrocatalytic protein electrodes by the simple compression of carbon nanotube and protein powders was investigated using a series of proteins including bilirubin oxidase (BOx), bovine serum albumin (BSA), catalase, cytochrome C (Cyt C), diaphorase, FAD-dependent glucose dehydrogenase (FAD-GDH), galactose oxidase (GAOx), glucose oxidase (GOx), horseradish peroxidase (HRP), laccase and urease. The isoelectric points (pI) of the proteins ranged from 3.5 to 12 and the molecular weights ranged from 12.4 to 480 kDa. The compression of diaphorase, laccase, BOx, FAD-GDH, catalase and urease gave mechanically stable biopellets in 0.1 M phosphate buffer pH 7.4. For Cyt C, BSA, GAOx, GOx, and HRP, contact with buffer destabilised the biopellet and induced a fast destruction of the composite. In parallel, stable redox pellets were obtained with differently charged redox mediators: hexaammineruthenium (III) chloride, potassium hexacyanoferrate or 1,4-naphthoquinone. Several parameters were explored to shed new light on the factors that determine biopellet stability. Stable biopellets capable of direct and mediated electron transfer were subsequently elaborated, for O 2 reduction and glucose oxidation, respectively, based on the simplified procedure that does not require stabilising protection membranes or holders on the contrary to existing methods.
Published: 2021
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14. Complexes of the Bis(di-tert-butyl-aniline)amine Pincer Ligand: The Case of Copper

Author: Christian Philouze, Florian Molton, Solène Gentil, Nicolas Leconte, Fabrice Thomas, Alan Le Goff, Département de Chimie Moléculaire - Chimie Inorganique Redox Biomimétique (DCM - CIRE ), Département de Chimie Moléculaire (DCM), Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Solar fuels, hydrogen and catalysis (SolHyCat), Laboratoire de Chimie et Biologie des Métaux (LCBM - UMR 5249), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA ), Département de Chimie Moléculaire - Chimie Inorganique Redox (DCM - CIRE ), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), and ANR-17-EURE-0003,CBH-EUR-GS,CBH-EUR-GS(2017)
Subjects: Iminosemiquinones, Electronic structure, Coordination sphere, Spin states, 010405 organic chemistry, Ligand, chemistry.chemical_element, Disproportionation, Radicals, [CHIM.INOR]Chemical Sciences/Inorganic chemistry, 010402 general chemistry, 01 natural sciences, Copper, 0104 chemical sciences, law.invention, Inorganic Chemistry, Crystallography, Deprotonation, chemistry, law, [CHIM.COOR]Chemical Sciences/Coordination chemistry, Electron paramagnetic resonance, Pincer ligand, Phenylenediamine
Abstract: International audience; TheN,N-bis(2-amino-3,5-di-tert-butylphenyl)amine pincer ligand was coordinated to copper. Depending on the copper source, a mononuclear complex1(+)or a trimer2could be isolated and were structurally characterized. Complex1(+)consists of two deprotonated iminobenzoquinone ligands coordinated to a Cu(I) center. Complex2is trinuclear with a (3:3) (M:L) stoichiometry, featuring a three-fold repetition of a unit made of a Cu(II) center coordinated to a tridentate ligand radical-dianion. In2, the metal ions are bridged by an anilido nitrogen. The coordination sphere of each copper is completed to four by a neighboring iminosemiquinone moiety. Complex1(+)belongs to an electron-transfer series. The paramagnetic complexes1and1(2+)were generated and characterized by EPR and Vis-NIR spectroscopy. Complex1exhibits an isotropic resonance at g = 2.00, which is reminiscent of Cu(I) iminosemiquinone species. The dication1(2+)exhibits a metal-based ground spin state and hence is described as a Cu(II) iminobenzoquinone complex. Both1and1(+)show a NIR band (954, 980 nm) of high intensity (> 20 mm(-1) cm(-1)) assigned to ligand-based charge transfer transitions. Two-electron reduction of1produces2via ligand release and disproportionation. Conversely, oxidation of2affords1(+). Finally, carbon-nanotube-supported complex2is active towards electrocatalytic reduction of H2O2.
Published: 2020
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15. Enhanced Electrochemiluminescence of Porphyrin-Based Metal–Organic Frameworks Controlled via Coordination Modulation

Author: Wen-Rong Cai, Huaiguo Xue, Haibo Zeng, Serge Cosnier, Dan Shan, Robert S. Marks, Xueji Zhang, Ben-Gurion University of the Negev (BGU), Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA ), Département de Chimie Moléculaire (DCM), Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Chemistry, 010401 analytical chemistry, Crystal growth, 010402 general chemistry, 01 natural sciences, Combinatorial chemistry, Porphyrin, 0104 chemical sciences, Analytical Chemistry, Solvent, chemistry.chemical_compound, Electrochemiluminescence, Metal-organic framework, [CHIM.COOR]Chemical Sciences/Coordination chemistry, Cyclic voltammetry, Hybrid material, Nanoscopic scale, ComputingMilieux_MISCELLANEOUS
Abstract: Precise control over the composition, morphology, and size of porphyrin-based metal-organic frameworks is challenging, but the extension of these hybrid materials will enable the creation of novel electrochemiluminescence (ECL) emitters. The coordination of various entities is made from Zn2+ ions and meso-tetra(4-carboxyphenyl)porphine (TCPP), modulated by both solvent and bathophenanthrolinedisulfonic acid disodium salt (BPS) as capping agent, resulting in limited crystal growth of Zn-TCPP in DMF/H2O (v/v, 1:1) and the formation of nanoscale TCPP-Zn-BPS. The role of BPS is also evaluated using Zn-TCPP and BPS-Zn-TCPP as controls, prepared in the absence of BPS and different coordinating sequences of ligands, respectively. The newly obtained TCPP-Zn-BPS exhibits a variety of different morphologies, as well as spectral and optoelectronic properties. The ECL behavior of TCPP-Zn-BPS is investigated by using H2O2 as co-reactant. The amplification of ECL is further studied by ECL spectroscopies and cyclic voltammetry, with the corresponding mechanism proposed.
Published: 2020
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16. Controllable Display of Sequential Enzymes on Yeast Surface with Enhanced Biocatalytic Activity toward Efficient Enzymatic Biofuel Cells

Author: Shuqin Fan, Serge Cosnier, Bo Liang, Xiangjiang Tang, Aihua Liu, Xinxin Xiao, Elisabeth Lojou, Lu Bai, Qingdao University, Bioénergétique et Ingénierie des Protéines (BIP ), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA ), Département de Chimie Moléculaire (DCM), Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Qingdao Institute of Marine Geology, China Geological Survey, Qingdao 266071, China, Université Paris Diderot - Paris 7 (UPD7), Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Qingdao University (QDU), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), and ANR-16-CE05-0024,Enzymor,Bases moléculaires de l'immobilisation fonctionnelle d'enzymes pour des biopiles performantes(2016)
Subjects: Bioelectric Energy Sources, Starch, Kinetics, Saccharomyces cerevisiae, 010402 general chemistry, 7. Clean energy, 01 natural sciences, Biochemistry, Catalysis, Glucose Oxidase, chemistry.chemical_compound, Colloid and Surface Chemistry, Enzyme Stability, Glucose oxidase, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, ComputingMilieux_MISCELLANEOUS, chemistry.chemical_classification, biology, General Chemistry, Enzymes, Immobilized, Combinatorial chemistry, Yeast, 0104 chemical sciences, [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics, Enzyme, chemistry, Biocatalysis, biology.protein, Glucan 1,4-alpha-Glucosidase, [CHIM.OTHE]Chemical Sciences/Other, Biosensor
Abstract: A precisely localized enzyme cascade was constructed by integrating two sequential enzymes, glucoamylase (GA) and glucose oxidase (GOx), on a yeast cell surface through an a-agglutinin receptor as the anchoring motif with cohesin-dockerin interaction. The overall catalytic activities of the combinant strains were significantly dependent on the assembly method, enzyme molecular size, enzyme order, and enzyme stoichiometry. The combinant strain with GA-DocC initially bound scaffoldin prior to GOx-DocT exhibited a higher overall reaction rate. The highest overall reaction rate (29.28 ± 1.15 nmol H2O2 min-1mL-1) was achieved when GA/GOx ratio was 2:1 with enzyme order: yeast-GA-GOx-GA, 4-fold enhancement compared to free enzyme mixture. Further, the first example of starch/O2 enzymatic biofuel cells (EBFCs) using codisplayed GA/GOx based bioanodes were assembled, demonstrating excellent direct biomass-to-electricity conversion. The optimized EBFC registered an open-circuit voltage of 0.78 V and maximum power density (Pmax) of 36.1 ± 2.5 μW cm-2, significantly higher than the Pmax for other starch/O2 EBFCs reported so far. Therefore, this work highlights rational organization of sequential enzymes for enhanced biocatalytic activity and stability, which would find applications in biocatalysis, enzymatic biofuel cells, biosensing, and bioelectro-synthesis.
Published: 2020
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17. Diazonium electrografting vs. physical adsorption of Azure A at carbon nanotubes for mediated glucose oxidation with FAD‐GDH

Author: Yuta Nishina, Clara Colomies, Michael Holzinger, Serge Cosnier, Fabien Giroud, Andrew J. Gross, Seiya Tsujimura, Shunya Tanaka, Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA ), Département de Chimie Moléculaire (DCM), Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Division of Materials Science, Faculty of Pure and Applied Sciences, University of Tsukuba
Subjects: Chemistry, [SDV]Life Sciences [q-bio], Azure A, 02 engineering and technology, Carbon nanotube, 010402 general chemistry, 021001 nanoscience & nanotechnology, Photochemistry, 01 natural sciences, 7. Clean energy, Catalysis, 0104 chemical sciences, law.invention, chemistry.chemical_compound, Adsorption, law, Electrochemistry, [CHIM]Chemical Sciences, 0210 nano-technology
Abstract: International audience; The electrochemical reduction of aryldiazonium salts is a versatile and direct route to obtain robust covalently-modified electrodes. We report here a comparative study of Azure-A modified carbon nanotube electrodes prepared by diazonium electrografting and by physical adsorption for bioelectrocatalytic glucose oxidation with fungal FAD-glucose dehydrogenase from Aspergillus sp. The electrografted and adsorbed electrodes exhibited different reversible electroactivity consistent with polymer-type and monomer-type phenothiazine surface assemblies, respectively. The electrografted Azure A electrodes exhibited superior mediated bioelectrocatalysis compared to the adsorbed Azure A electrodes. A more than 10-fold higher catalytic current up to 2 mA cm-2 at 0.2 V vs. Ag/AgCl together with a similarly low onset potential of-0.05 V vs. Ag/AgCl was observed at the electrografted electrodes. Faster estimated electron transfer kinetics and a +200 mV potential shift for the polymer-type redox couple vs. the adsorbed monomer-type couple underlines the favourable driving force for mediated electron transfer with the buried FAD active site for the diazonium-derived bioelectrode. Keywords: Phenothiazine redox mediator • FAD dependant glucose dehydrogenase • biofuel cell anode • enzymatic bioelectrode • bioelectrocatalysis 2
Published: 2020
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18. Functionalized tungsten disulfide nanotubes for dopamine and catechol detection in a tyrosinase-based amperometric biosensor design

Author: Serge Cosnier, Michael Holzinger, Chantal Gondran, Jean-Paul Lellouche, Quentin Palomar, Département de Chimie Moléculaire (DCM), Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA ), and Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Surface Properties, Dopamine, Tyrosinase, Inorganic chemistry, Tungsten disulfide, Catechols, Biomedical Engineering, Biosensing Techniques, 02 engineering and technology, Carbon nanotube, Sulfides, Glassy carbon, 010402 general chemistry, 01 natural sciences, Michaelis–Menten kinetics, law.invention, chemistry.chemical_compound, law, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Humans, General Materials Science, Particle Size, Electrodes, ComputingMilieux_MISCELLANEOUS, Catechol, Nanotubes, Monophenol Monooxygenase, Substrate (chemistry), Electrochemical Techniques, Equipment Design, General Chemistry, General Medicine, Tungsten Compounds, 021001 nanoscience & nanotechnology, 0104 chemical sciences, [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry, chemistry, 0210 nano-technology, Biosensor
Abstract: WS2 nanotubes functionalized with carboxylic acid functions (WS2-COOH) were used for improved immobilization of the enzyme tyrosinase in order to form an electrochemical biosensor towards catechol and dopamine. The nanotubes were deposited on glassy carbon electrodes using a dispersion-filtration-transfer procedure to assure the reproducibility of the deposits. After the electrochemical and morphological characterization of these WS2-COOH nanotube deposits, the formed biosensors showed very satisfying performance towards catechol detection with a linear range of 0.6-70 μmol L-1 and a sensitivity of 10.7 ± 0.2 mA L mol-1. The apparent Michaelis Menten constant of this system is slightly lower than the KM value of tyrosinase in solution, reflecting an excellent accessibility of the active site of the enzyme combined with a good mass transport of the target molecule through the deposit. For dopamine detection, we observed an accumulation of this substrate due to electrostatic interactions between the amine function of dopamine and the carboxylic acid groups of the nanotubes. This led to improved signal capture at low dopamine concentrations. With linear ranges of 0.5-10 μmol L-1 and 10-40 μmol L-1, and respective sensitivities of 6.2 ± 0.7 mA L mol-1 and 3.4 ± 0.4 mA L mol-1, the overall sensor performance is within the average of comparable results using carbon nanotubes. Nonetheless, the simplified handling of these nanotubes and their reduced environmental impact make these WS2-COOH nanotubes a promising nanomaterial for biosensing applications.
Published: 2020
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19. Solubilized Enzymatic Fuel Cell (SEFC) for Quasi-Continuous Operation Exploiting Carbohydrate Block Copolymer Glyconanoparticle Mediators

Author: Jules L. Hammond, Fabien Giroud, Serge Cosnier, Redouane Borsali, Andrew J. Gross, Christophe Travelet, Département de Chimie Moléculaire (DCM), Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Centre de Recherches sur les Macromolécules Végétales (CERMAV ), and Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Centre National de la Recherche Scientifique (CNRS)
Subjects: Continuous operation, Energy Engineering and Power Technology, chemistry.chemical_element, 02 engineering and technology, 010402 general chemistry, 7. Clean energy, 01 natural sciences, Oxygen, Redox, [SPI.MAT]Engineering Sciences [physics]/Materials, Materials Chemistry, [CHIM]Chemical Sciences, ComputingMilieux_MISCELLANEOUS, chemistry.chemical_classification, Renewable Energy, Sustainability and the Environment, Chemistry, food and beverages, Substrate (chemistry), [CHIM.MATE]Chemical Sciences/Material chemistry, Carbohydrate, 021001 nanoscience & nanotechnology, Combinatorial chemistry, 0104 chemical sciences, Quinone, [CHIM.POLY]Chemical Sciences/Polymers, Fuel Technology, Enzyme, Chemistry (miscellaneous), Biofuel, 0210 nano-technology
Abstract: International audience; Enzymatic biofuel cells are ecofriendly power sources that can deliver μW−mW outputs from renewable substrates, but their stability is a major issue owing to enzyme fragility. The vast majority of reported biofuel cells can only generate power continuously for relatively short periods of time. Here we report a novel "solubilized enzymatic fuel cell (SEFC)" concept for continuous long-term operation. Avoiding surface immobilization techniques allows bio- catalytic activity to be easily restored or replenished. The biofuel cell exploits freely diffusing enzymes and β-cyclodextrin-coated glycona- noparticles with entrapped quinone and thiazoline redox mediators, for mediated glucose and oxygen conversion. The cell was designed with permselective membranes to enable substrate and proton diffusion while trapping the enzymes and glyconanoparticles in separate compartments. The SEFC exhibited a peak power loss of only 26.3% after 7 days of continuous charge−discharge cycling at 50 μA; thus, SEFCs may be envisaged to power lab-on-a-chip devices for periods of several weeks.
Published: 2018
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20. Carbon nanotube-based flexible biocathode for enzymatic biofuel cells by spray coating

Author: Didier Chaussy, Ahlem Romdhane, Noémie Lalaoui, Nadège Reverdy-Bruas, Serge Cosnier, Naceur Belgacem, Alan Le Goff, Michael Holzinger, Awatef Ben Tahar, Systèmes Nanobiotechnologiques et Biomimétiques (TIMC-IMAG-SyNaBi), Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 (TIMC-IMAG), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Laboratoire Génie des procédés papetiers (LGP2 ), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut National Polytechnique de Grenoble (INPG)-Centre National de la Recherche Scientifique (CNRS), Département de Chimie Moléculaire (DCM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), and Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])
Subjects: Materials science, Carbon nanotubes, Energy Engineering and Power Technology, 02 engineering and technology, Carbon nanotube, 010402 general chemistry, Electrochemistry, 01 natural sciences, 7. Clean energy, law.invention, Immobilization, law, Conductive ink, [CHIM]Chemical Sciences, Electrical and Electronic Engineering, Physical and Theoretical Chemistry, Laccase, Renewable Energy, Sustainability and the Environment, Biofuel cell, Dispersion, 021001 nanoscience & nanotechnology, Enzymes, 0104 chemical sciences, Chemical engineering, Electrode, Spray coating, 0210 nano-technology, Dispersion (chemistry), Current density, Layer (electronics)
Abstract: International audience; Enzymatic biofuel cells are emerging technologies which gain a lot of interest as a power source for implantable devices. Effective enzyme wiring and simplification of the cell architecture are the main limitations of the fuel cell development nowadays. In the present study, spray coating was used as a reliable process to produce flexible biocathodes. That was achieved through spray coating of a conductive ink formulated by surfactant assisted carbon nanotubes dispersion. A thin continuous layer of carbon nanotubes (CNTs) was successfully coated on top of a gas diffusion layer paper. The film thickness was modulated by varying the CNT load in the ink and the number of deposited layers. It varied between 1 and 7.8 μm for a number of deposited layers between 5 and 100. Laccase enzyme was then wired to printed CNT electrodes thanks to the host-guest interaction between the hydrophobic pocket of laccase and pyrene-adamantane. Electrochemical investigations showed that laccase was effectively wired to the CNTs and presented a favorable orientation toward oxygen reduction. The biocathode current density was dependent of the number of deposited CNT layers and reached an optimum at 170 μA cm−2 for 50 deposited layers.
Published: 2018
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21. Towards eco-friendly power sources: In series connected glucose biofuel cells power a disposable ovulation test

Author: Caroline Abreu, Yannig Nedellec, Alan Le Goff, Serge Cosnier, Olivier Ondel, Michael Holzinger, François Buret, Ampère, Département Bioingénierie (BioIng), Ampère (AMPERE), École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-École Centrale de Lyon (ECL), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département de Chimie Moléculaire (DCM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Ampère, Département Energie Electrique (EE), Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Région Auvergne-Rhône-AlpesPlate-forme Chimie NanoBio http://icmg.ujf-grenoble.fr/ICMG-SITE/ICMG/index.php?page=ICMG-SITE/IC, and ANR-11-LABX-0003,ARCANE,Grenoble, une chimie bio-motivée(2011)
Subjects: Battery (electricity), Materials science, Ovulation test, [SDV]Life Sciences [q-bio], Disposable device, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, 7. Clean energy, In series connected stack, Automotive engineering, Stack (abstract data type), Materials Chemistry, Electrical and Electronic Engineering, Eco-Friendly power source, Instrumentation, [SDE.IE]Environmental Sciences/Environmental Engineering, Open-circuit voltage, [SPI.NRJ]Engineering Sciences [physics]/Electric power, Metals and Alloys, 021001 nanoscience & nanotechnology, Condensed Matter Physics, Environmentally friendly, Enzymatic glucose biofuel cells, 0104 chemical sciences, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Power (physics), Biofuel, 0210 nano-technology, Voltage drop, Biofuel Cells
Abstract: International audience; A disposable ovulation test is operated using a flow stack of 5 in series connected glucose biofuel cells giving an open circuit voltage (OCV) of 3.5 V. Such tests consume at total around 60 μWh of energy and in average 1.7 mW of power during one run of around 12 min which are supplied by a CR1220 3 V lithium button battery in the commercialized device. We replaced this battery by the glucose biofuel cell stack and observed during the measurements and display steps peak currents up to 1.4 mA inducing a voltage drop down to 2.8 V during few seconds (4 mW of power consumption). Even such power peaks are supported by the presented biofuel cell stack assuring the necessary operational cell voltage and current. After the measurements towards Luteinizing Hormone (LH) detection the display remains turned on for 8 min and consumes just 2 μW which allows to reestablish the initial OCV and to run a further test. The presented results demonstrate as proof of concept the suitability of eco-friendly enzymatic glucose biofuel cells as alternative power source for disposable devices.
Published: 2018
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22. Glucose oxidase bioanodes for glucose conversion and H2O2 production for horseradish peroxidase biocathodes in a flow through glucose biofuel cell design

Author: Olivier Ondel, Michael Holzinger, Caroline Abreu, Serge Cosnier, Alan Le Goff, François Buret, Yannig Nedellec, Ampère, Département Bioingénierie (BioIng), Ampère (AMPERE), École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-École Centrale de Lyon (ECL), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), Département de Chimie Moléculaire (DCM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Ampère, Département Energie Electrique (EE), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), platform Chimie NanoBio ICMG FR 2607 (PCN-ICMG), Institute Carnot PolyNat at Grenoble, and ANR-11-LABX-0003,ARCANE,Grenoble, une chimie bio-motivée(2011)
Subjects: H2O2, Energy Engineering and Power Technology, 02 engineering and technology, Electrolyte, 010402 general chemistry, 01 natural sciences, 7. Clean energy, Horseradish peroxidase, law.invention, chemistry.chemical_compound, law, [CHIM]Chemical Sciences, Glucose oxidase, Electrical and Electronic Engineering, Physical and Theoretical Chemistry, Cellulose, biology, Renewable Energy, Sustainability and the Environment, Chemistry, Substrate (chemistry), 021001 nanoscience & nanotechnology, Biofuel cells, Cathode, 0104 chemical sciences, Anode, Glucose, Membrane, Flow stacks, Chemical engineering, biology.protein, 0210 nano-technology
Abstract: International audience; Bioelectrocatalytic carbon nanotube pellets comprising glucose oxidase (GOx) at the anode and horseradish peroxidase (HRP) at the cathode were integrated in a glucose/H2O2 flow-through fuel cell setup. The porous bioelectrodes, separated with a cellulose membrane, were assembled in a design allowing the fuel/electrolyte flow through the entire fuel cell with controlled direction. An air saturated 5 mmol L−1 glucose solution was directed through the anode where glucose is used for power conversion and for the enzymatic generation of hydrogen peroxide supplying the HRP biocathode with its substrate. This configuration showed an open circuit voltage (OCV) of 0.6 V and provided 0.7 ± 0.035 mW at 0.41 V. Furthermore, different charge/discharge cycles at 500 Ω and 3 kΩ were applied to show the long term stability of this setup producing 290 μW h (1.04 J) of energy after 48 h. The biofuel cell design further allows a convenient assembly of several glucose biofuel cells in reduced volumes and its connection in parallel or in series.
Published: 2018
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23. Buckypaper bioelectrodes: emerging materials for implantable and wearable biofuel cells

Author: Michael Holzinger, Andrew J. Gross, Serge Cosnier, Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques [2016-2019] (DCM - BEA [2016-2019]), Département de Chimie Moléculaire [2016-2019] (DCM [2016-2019]), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Département de Chimie Moléculaire (DCM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), and Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])
Subjects: chemistry.chemical_classification, Materials science, Biocompatibility, Renewable Energy, Sustainability and the Environment, Nanotechnology, Buckypaper, 02 engineering and technology, Carbon nanotube, Polymer, 010402 general chemistry, 021001 nanoscience & nanotechnology, 7. Clean energy, 01 natural sciences, Pollution, 0104 chemical sciences, law.invention, Nuclear Energy and Engineering, chemistry, law, [CHIM]Chemical Sciences, Environmental Chemistry, 0210 nano-technology, Energy harvesting, ComputingMilieux_MISCELLANEOUS, Biofuel Cells
Abstract: Carbon nanotubes (CNTs) have been widely exploited for the development of enzymatic biofuel cells with sufficient power densities in the μW to mW range for operating low-power bioelectronic devices from renewable substrates. Buckypaper is a randomly oriented self-supporting film of carbon nanotubes, resembling an electronic paper, with excellent prospects for the construction of high performance enzymatic electrodes for use in biofuel cells. Attractive properties of buckypaper materials include large specific surface areas, high electrical conductivity, flexibility, biocompatibility, scalable production and the ability for efficient electron transfer with enzymes. Buckypapers are ideal self-supporting frameworks for enzymes and guest molecules such as metals, polymers and redox molecules, permitting the development of a wide range of catalytic bioelectrode interfaces. This review summarizes recent developments and advances of buckypaper bioelectrodes as an emerging component for body-integrated energy harvesting biodevices.
Published: 2018
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24. Fe-MOGs-based enzyme mimetic and its mediated electrochemiluminescence for in situ detection of H2O2 released from Hela cells

Author: Dan Shan, Junji Li, Xueji Zhang, Robert S. Marks, Ling-Yu Ruan, Serge Cosnier, Li-Ping Zong, Jun-Song Wang, Nanjing University of Science and Technology (NJUST), Ben-Gurion University of the Negev (BGU), Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA ), Département de Chimie Moléculaire (DCM), and Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Biomedical Engineering, Biophysics, hydrogen peroxide, 02 engineering and technology, 01 natural sciences, Horseradish peroxidase, Catalysis, Luminol, Hela cells, HeLa, chemistry.chemical_compound, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Electrochemistry, Electrochemiluminescence, [CHIM.COOR]Chemical Sciences/Coordination chemistry, chemistry.chemical_classification, Detection limit, biology, Chemistry, electrochemiluminescence (ECL), 010401 analytical chemistry, 1 10-phenanthroline-2 9-dicarboxylic acid (PDA), General Medicine, enzyme mimetic, 021001 nanoscience & nanotechnology, biology.organism_classification, Combinatorial chemistry, 0104 chemical sciences, Enzyme, biology.protein, 0210 nano-technology, Biosensor, Biotechnology
Abstract: International audience; Enzyme mimetics have attracted wide interest due to their inherent enzyme-like activity and unique physicochemical properties, as well as promising applications in disease diagnosis, treatment and monitoring. Inspired by the attributes of nonheme iron enzymes, synthetic models were designed to mimic their capability and investigate the catalytic mechanisms. Herein, metal-organic gels (Fe-MOGs) with horseradish peroxidase (HRP) like Fe-NX structure were successfully synthesized though the coordination between iron and 1,10-phenanthroline-2,9-dicarboxylic acid (PDA) and exhibited excellent peroxidase-like activity. Its structure-activity relationship and the in-situ electrochemiluminescence (ECL) detection of H2O2 secreted by Hela cells were further investigated. The highly dispersed Fe-NX active sites inside Fe-MOGs were able to catalyze the decomposition of H2O2 into large amounts of reactive oxygen species (ROS) via a Fenton-like reaction under a low overpotential. Due to the accumulation of ROS free radicals, the luminol ECL emission was significantly amplified. A proof-ofconcept biosensor was constructed with a detection limit as low as 2.2 nM and a wide linear range from 0.01 to 40 μM. As a novel metal organic gels based enzyme mimetic, Fe-MOGs show great promises in early cancer detection and pathological process monitoring.
Published: 2021
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25. Nanostructured photoactivatable electrode surface based on pyrene diazirine

Author: Robert S. Marks, Serge Cosnier, Oana Hosu, Robert Săndulescu, Kamal Elouarzaki, Karine Gorgy, Cecilia Cristea, Département de Chimie Moléculaire (DCM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Analytical Chemistry Department, University of Medicine and Pharmacy, Université Cluj-Napoca, facultatea, and Ben-Gurion University of the Negev (BGU)
Subjects: biology, 010405 organic chemistry, chemistry.chemical_element, 010402 general chemistry, Photochemistry, Electrochemistry, 01 natural sciences, 0104 chemical sciences, lcsh:Chemistry, chemistry.chemical_compound, lcsh:Industrial electrochemistry, lcsh:QD1-999, chemistry, Covalent bond, Diazirine, Photografting, biology.protein, [CHIM]Chemical Sciences, Pyrene, Glucose oxidase, Platinum, ComputingMilieux_MISCELLANEOUS, Derivative (chemistry), lcsh:TP250-261
Abstract: An original versatile methodology for molecular grafting on different surfaces via the photoinduced formation of a covalent bond based on a diazirine group is reported. The synthesis and electrochemical behavior of a new diazirine derivative which acts as a molecular linking bridge bearing both a photoactivatable covalent binding group (diazirine) and a non-covalent binding group (pyrene) is described. The resulting pyrene-diazirine was electropolymerized onto a platinum electrode and under UV irradiation was successfully used to graft ferrocenemethanol and glucose oxidase (model small molecule and protein, respectively), conferring specific properties on the resulting materials. Furthermore, the immobilization of the diazirine derivative onto multi-walled carbon nanotubes (MWCNT) by π-stacking interaction or by electropolymerization onto both bare and MWCNT-coated platinum electrodes enabled photografting of glucose oxidase. Keywords: Diazirine, Pyrene, Photografting, Electropolymerization, Glucose oxidase, Carbon nanotubes
Published: 2017
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26. Tuning the redox potential of vitamin K3 derivatives by oxidative functionalization using a Ag(<scp>i</scp>)/GO catalyst

Author: Hassan M.A. Hassan, Michael Holzinger, Soliman I. El-Hout, Ibrahim A. Ibrahim, Hideyuki Suzuki, Farid A. Harraz, E.A. El-Sharkawy, Seiya Tsujimura, Yuta Nishina, Said M. El-Sheikh, Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), Département de Chimie Moléculaire (DCM), and Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])
Subjects: 010405 organic chemistry, Chemistry, Metals and Alloys, Vitamin K3, General Chemistry, Oxidative phosphorylation, Alkylation, 010402 general chemistry, Photochemistry, 01 natural sciences, Redox, Catalysis, 0104 chemical sciences, 3. Good health, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Materials Chemistry, Ceramics and Composites, Molecule, Surface modification, HOMO/LUMO, ComputingMilieux_MISCELLANEOUS
Abstract: We propose herein initial results to develop optimum redox mediators by the combination of computational simulation and catalytic functionalization of the core structure of vitamin K3. We aim to correlate the calculated energy value of the LUMO of different vitamin K3 derivatives with their actual redox potential. For this, we optimized the catalytic alkylation of 1,4-naphthoquinones with a designed Ag(i)/GO catalyst and synthesized a series of molecules.
Published: 2017
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27. A diethyleneglycol-pyrene-modified Ru(II) catalyst for the design of buckypaper bioelectrodes and the wiring of glucose dehydrogenases

Author: Serge Cosnier, Luminita Fritea, Andrew J. Gross, Alan Le Goff, Karine Gorgy, Bertrand Reuillard, Département de Chimie Moléculaire (DCM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Centre National de la Recherche Scientifique (CNRS), Laboratoire d'électrochimie organique et de photochimie redox (LEOPR), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS), Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), and Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])
Subjects: Chemistry, Buckypaper, 02 engineering and technology, Carbon nanotube, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, Catalysis, 0104 chemical sciences, law.invention, chemistry.chemical_compound, Chemical engineering, law, Glucose dehydrogenase, Electrochemistry, Pyrene, [CHIM]Chemical Sciences, 0210 nano-technology, ComputingMilieux_MISCELLANEOUS
Abstract: International audience; A novel water-soluble Ru complex bearing phenanthrolinequinone ligands, diethyleneglycol linkers and pyrene anchoring groups was synthesized. Electrical wiring of NAD-and FAD-dehydrogenases is demonstrated via NADH oxidation and mediated electron transfer respectively. Bioelectrocatalysis is facilitated by the flexible and hydrophilic PEG groups, and strong binding of pyrene groups with the carbon nanotubes (CNTs) of the electrodes. Furthermore, owing to the surfactant properties of the PEG-modified complex, high quality CNT dispersions were obtained for homogeneous buckypaper preparation.
Published: 2019
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28. Electrospun Biomaterials

Author: Serge Cosnier, Issei Otsuka, Redouane Borsali, Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), Département de Chimie Moléculaire (DCM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Centre de Recherches sur les Macromolécules Végétales (CERMAV ), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), and Otsuka, Issei
Subjects: [SDV.IB.BIO] Life Sciences [q-bio]/Bioengineering/Biomaterials, [CHIM.POLY] Chemical Sciences/Polymers, [CHIM.MATE] Chemical Sciences/Material chemistry, [CHIM.POLY]Chemical Sciences/Polymers, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [CHIM.MATE]Chemical Sciences/Material chemistry, [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials, ComputingMilieux_MISCELLANEOUS, [SDV.BIO] Life Sciences [q-bio]/Biotechnology
Abstract: International audience
Published: 2019

29. 1. Buckypapers for bioelectrochemical applications

Author: Michael Holzinger, Serge Cosnier, Andrew J. Gross, Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), Département de Chimie Moléculaire (DCM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), and Centre National de la Recherche Scientifique (CNRS)
Subjects: Materials science, 010405 organic chemistry, [CHIM]Chemical Sciences, 010402 general chemistry, 01 natural sciences, ComputingMilieux_MISCELLANEOUS, 0104 chemical sciences
Abstract: International audience
Published: 2019
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30. Bio-electrode pour la detection et/ou l'oxydation du glucose et son procede de fabrication et dispositif la comprenant

Author: Giroud, Fabien, Gross, Andrew James, Cosnier, Serge, GROSS, Andrew, Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), Département de Chimie Moléculaire (DCM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), and Centre National de la Recherche Scientifique (CNRS)
Subjects: Electrochimie, [CHIM] Chemical Sciences, technology, industry, and agriculture, Electrochemistry, [CHIM]Chemical Sciences, A61B5/1486 Measuring characteristics of blood in vivo, e.g. gas concentration, pH value, Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue using enzyme electrodes, e.g. with immobilised oxidase, humanities
Abstract: The invention concerns a bioelectrode and a device comprising same, for detecting or oxidising glucose. The bioelectrode of the invention comprises a layer of carbon nanotubes to which aromatic molecules are bonded, and FAD-GDH enzymes being adsorbed on the aromatic molecules. The invention applies to the field of biosensors and biofuel cells in particular.
Published: 2019

31. Enzymatic versus Electrocatalytic Oxidation of NADH at Carbon-Nanotube Electrodes Modified with Glucose Dehydrogenases: Application in a Bucky-Paper-Based Glucose Enzymatic Fuel Cell

Author: Sadagopan Krishnan, Noémie Lalaoui, Michael Holzinger, Nicholas Means, Charuksha Walgama, Alan Le Goff, Serge Cosnier, Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), Département de Chimie Moléculaire (DCM), and Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])
Subjects: 010405 organic chemistry, Inorganic chemistry, chemistry.chemical_element, Buckypaper, Carbon nanotube, 010402 general chemistry, 01 natural sciences, 7. Clean energy, Catalysis, 0104 chemical sciences, Ruthenium, law.invention, Anode, chemistry, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, law, Glucose dehydrogenase, Diaphorase, Electrode, Electrochemistry, ComputingMilieux_MISCELLANEOUS
Abstract: In this work, we have designed and compared functionalized carbon-nanotube-based electrodes for the electrocatalytic oxidation of NADH. We show that oxidation of NADH at neutral pH values can be performed by pristine multi-walled carbon nanotubes (MWCNT) and directly wired diaphorase or ruthenium phenanthrolinequinone molecular catalysts. Glucose dehydrogenase was immobilized on these MWCNT electrodes by an activated-ester-modified pyrene linker. Glucose-oxidizing bioelectrodes were compared and integrated into a bucky-paper-based glucose/O2 biofuel cell, using a bilirubin-oxidase-modified bucky-paper cathode. The best configuration was obtained using a NADH-oxidizing molecular catalyst at the anode. The biofuel cell exhibits an open-circuit voltage of 0.62 V and delivers a power output of 0.47 mW cm−2.
Published: 2016
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32. Diazonium Functionalisation of Carbon Nanotubes for Specific Orientation of Multicopper Oxidases: Controlling Electron Entry Points and Oxygen Diffusion to the Enzyme

Author: Michael Holzinger, Noémie Lalaoui, Alan Le Goff, Serge Cosnier, Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), Département de Chimie Moléculaire (DCM), and Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])
Subjects: Oxidoreductases Acting on CH-CH Group Donors, Surface Properties, Inorganic chemistry, Molecular Conformation, chemistry.chemical_element, Electrons, 02 engineering and technology, Carbon nanotube, 010402 general chemistry, Electrocatalyst, 01 natural sciences, Oxygen, Catalysis, law.invention, Diffusion, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, law, Electrodes, ComputingMilieux_MISCELLANEOUS, chemistry.chemical_classification, Diazonium Compounds, biology, Nanotubes, Carbon, Chemistry, Organic Chemistry, Electrochemical Techniques, General Chemistry, 021001 nanoscience & nanotechnology, biology.organism_classification, Copper, 0104 chemical sciences, Hypocreales, Electrode, Myrothecium verrucaria, Oxidoreductases, 0210 nano-technology, Oxidation-Reduction
Abstract: We report the controlled orientation of bilirubin oxidases (BOD) from Myrothecium verrucaria on multiwalled carbon nanotubes (MWCNTs) functionalised by electrografting of 6-carboxynaphthalenediazonium and 4-(2-aminoethyl)benzenediazonium salts. On negatively charged naphthoate-modified MWCNTs, a high-potential (0.44 V vs. SCE) oxygen reduction electrocatalysis is observed, occurring via the T1 copper centre. On positively charged ammonium-modified MWCNTs, a low-potential (0.15 V) oxygen reduction electrocatalysis is observed, occurring through a partially oxidised state of the T2/T3 trinuclear copper cluster. Finally, chemically modified naphthoate MWCNTs exhibit high bioelectrocatalytic current densities of 3.9 mA cm(-2) under air at gas-diffusion electrode.
Published: 2016
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33. Direct Electron Transfer between a Site-Specific Pyrene-Modified Laccase and Carbon Nanotube/Gold Nanoparticle Supramolecular Assemblies for Bioelectrocatalytic Dioxygen Reduction

Author: Michael Holzinger, Yasmina Mekmouche, Noémie Lalaoui, Thierry Tron, Pierre Rousselot-Pailley, Serge Cosnier, Viviane Robert, Alan Le Goff, Reynaldo Villalonga, Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), Département de Chimie Moléculaire (DCM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Institut des Sciences Moléculaires de Marseille (ISM2), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Institut de Chimie du CNRS (INC), and Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Inorganic chemistry, Population, Nanoparticle, 02 engineering and technology, Carbon nanotube, 010402 general chemistry, Electrocatalyst, 01 natural sciences, Reductive amination, Catalysis, law.invention, chemistry.chemical_compound, Electron transfer, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, law, education, ComputingMilieux_MISCELLANEOUS, education.field_of_study, General Chemistry, 021001 nanoscience & nanotechnology, 0104 chemical sciences, chemistry, Chemical engineering, Colloidal gold, Pyrene, 0210 nano-technology
Abstract: Strategies to maximize direct electron transfer (DET) between redox enzymes and electrodes include the oriented immobilization of enzymes onto an electroactive surface. Here, we present a strategy for achieving a controlled orientation of a fungal laccase on carbon nanotube-based electrodes. A homogeneous population of pyrene-modified laccase is obtained via the reductive amination of a unique surface accessible lysine residue engineered near the T1 copper center of the enzyme. Immobilization of the site-specific functionalized enzyme is achieved either via π-stacking of pyrene on pristine CNT electrodes or through pyrene/β-cyclodextrin host guest interactions on β-cyclodextrin-modified gold nanoparticles (β-CD-AuNPs). Contrasting with unmodified and nonspecifically modified (pyrene-NHS) laccase-electrodes, an efficient DET is obtained at these nanostructured assemblies. Modeling the direct bioelectrocatalysis of dioxygen reduction reveals a heterogeneity in ET rates on MWCNT electrodes wheras β-CD-AuNPs ...
Published: 2016
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34. Dumbbell-shaped carbon quantum dots/AuNCs nanohybrid as an efficient ratiometric fluorescent probe for sensing cadmium (II) ions and l-ascorbic acid

Author: Dan Shan, Wen Jun Niu, Xueji Zhang, Sheng Yuan Deng, Rong Hui Zhu, Serge Cosnier, Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), Département de Chimie Moléculaire (DCM), and Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])
Subjects: Detection limit, Cadmium, Materials science, Quenching (fluorescence), Fluorophore, Inorganic chemistry, chemistry.chemical_element, 02 engineering and technology, General Chemistry, 010402 general chemistry, 021001 nanoscience & nanotechnology, Ascorbic acid, Photochemistry, 01 natural sciences, Fluorescence, 0104 chemical sciences, Nanoclusters, chemistry.chemical_compound, chemistry, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, General Materials Science, 0210 nano-technology, ComputingMilieux_MISCELLANEOUS, Histidine
Abstract: In this work, for the first time, an efficient carbon quantum dots/gold nanoclusters (CQDs/AuNCs) nanohybrid based ratiometric fluorescent probe for sensitive and selective sensing of cadmium (II) ions and l-ascorbic acid (AA) has been established. The blue emissive CQDs, prepared by a one-step hydrothermal treatment with alanine and histidine, play the role of reference fluorophore. The red emissive AuNCs, capped by 11-mercaptoundecanoic acid (MUA), serve as a specific recognition element. Different from MUA-capped AuNCs, the proposed CQDs/AuNCs nanohybrid exhibits high selectivity and sensitivity to cadmium (II) ions with a detection limit of 32.5 nM, which is attributed to the static quenching and the inner filter effect (IFE) of Cd2+ to CQDs/AuNCs nanohybrid. Unbelievable, in the presence of AA, the above-quenched sensor is recovered gradually. The proposed fluorescence sensor displays an enhanced performance for AA determination in the range from 0.15 to 15 μM (R = 0.995) with a detection limit down to 0.105 μM. More importantly, this fluorescent “on–off–on” method also offers the excellent performance to Cd2+ and AA detection in lake water and in human serum, respectively.
Published: 2016
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35. Cubic PdNP-based air-breathing cathodes integrated in glucose hybrid biofuel cells

Author: Fabien Giroud, J.J.S. Acuña, Michael Holzinger, D. Faggion Junior, Josiel B. Domingos, Raoudha Haddad, C. E. Maduro de Campos, Serge Cosnier, Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), Département de Chimie Moléculaire (DCM), and Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])
Subjects: Materials science, Open-circuit voltage, Reducing agent, Nanotechnology, 02 engineering and technology, Glassy carbon, 010402 general chemistry, 021001 nanoscience & nanotechnology, Ascorbic acid, 7. Clean energy, 01 natural sciences, Cathode, 0104 chemical sciences, law.invention, Anode, Chemical engineering, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, law, Glucose dehydrogenase, General Materials Science, Limiting oxygen concentration, 0210 nano-technology, ComputingMilieux_MISCELLANEOUS
Abstract: Cubic Pd nanoparticles (PdNPs) were synthesized using ascorbic acid as a reducing agent and were evaluated for the catalytic oxygen reduction reaction. PdNPs were confined with multiwalled carbon nanotube (MWCNT) dispersions to form black suspensions and these inks were dropcast onto glassy carbon electrodes. Different nanoparticle sizes were synthesized and investigated upon oxygen reduction capacities (onset potential and electrocatalytic current densities) under O2 saturated conditions at varying pH values. Strong evidence of O2 diffusion limitation was demonstrated. In order to overcome oxygen concentration and diffusion limitations in solution, we used a gas diffusion layer to create a PdNP-based air-breathing cathode, which delivered -1.5 mA cm(-2) at 0.0 V with an onset potential of 0.4 V. This air-breathing cathode was combined with a specially designed phenanthrolinequinone/glucose dehydrogenase-based anode to form a complete glucose/O2 hybrid bio-fuel cell providing an open circuit voltage of 0.554 V and delivering a maximal power output of 184 ± 21 μW cm(-2) at 0.19 V and pH 7.0.
Published: 2016
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36. Long duration stabilization of porous silicon membranes in physiological media: Application for implantable reactors

Author: Frederic Gaillard, Donald K. Martin, Chantal Gondran, Marie-Line Cosnier, Guillaume Nonglaton, Philippe Cinquin, Jean-Pierre Alcaraz, Guillaume Costa, Abdoullatif Baraket, Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Systèmes Nanobiotechnologiques et Biomimétiques (TIMC-IMAG-SyNaBi), Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications Grenoble - UMR 5525 (TIMC-IMAG), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Université Grenoble Alpes (UGA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Université Grenoble Alpes (UGA), Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA ), Département de Chimie Moléculaire (DCM), Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Gestes Medico-chirurgicaux Assistés par Ordinateur (TIMC-GMCAO), Translational Innovation in Medicine and Complexity / Recherche Translationnelle et Innovation en Médecine et Complexité - UMR 5525 (TIMC ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Gestes Médico-chirurgicaux Assistés par Ordinateur (TIMC-IMAG-GMCAO), and ANR-17-EURE-0003,CBH-EUR-GS,CBH-EUR-GS(2017)
Subjects: Silicon, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Time Factors, Materials science, Diffusion, Bioengineering, 02 engineering and technology, 010402 general chemistry, Porous silicon, 01 natural sciences, Fluorescence, law.invention, Biomaterials, Bioreactors, law, Immersion (virtual reality), ComputingMilieux_MISCELLANEOUS, Filtration, chemistry.chemical_classification, Escherichia coli Proteins, Biomolecule, Membranes, Artificial, Prostheses and Implants, Silanes, 021001 nanoscience & nanotechnology, Culture Media, 0104 chemical sciences, Glucose, Membrane, chemistry, Chemical engineering, Mechanics of Materials, PEGylation, Nanoparticles, Surface modification, Fluorescein, 0210 nano-technology, Porosity
Abstract: The natural biodegradabilty of porous silicon (pSi) in physiological media limits its wider usage for implantable systems. We report the stabilization of porous silicon (pSi) membranes by chemical surface oxidation using RCA1 and RCA2 protocols, which was followed by a PEGylation process using a silane-PEG. These surface modifications stabilized the pSi to allow a long period of immersion in PBS, while leaving the pSi surface sufficiently hydrophilic for good filtration and diffusion of several biomolecules of different sizes without any blockage of the pSi structure. The pore sizes of the pSi membranes were between 5 and 20 nm, with the membrane thickness around 70 μm. The diffusion coefficient for fluorescein through the membrane was 2 × 10−10 cm2 s−1, and for glucose was 2.2 × 10−9 cm2 s−1. The pSi membrane maintained that level of glucose diffusion for one month of immersion in PBS. After 2 months immersion in PBS the pSi membrane continued to operate, but with a reduced glucose diffusion coefficient. The chemical stabilization of pSi membranes provided almost 1 week stable and functional biomolecule transport in blood plasma and opens the possibility for its short-term implantation as a diffusion membrane in biocompatible systems.
Published: 2020
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37. Beyond the hype surrounding biofuel cells: What's the future of enzymatic fuel cells?

Author: Fabien Giroud, Michael Holzinger, Andrew J. Gross, Serge Cosnier, Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), Département de Chimie Moléculaire (DCM), and Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])
Subjects: Chemistry, 02 engineering and technology, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, 7. Clean energy, 0104 chemical sciences, Analytical Chemistry, Electrochemistry, Fuel cells, [CHIM]Chemical Sciences, Biochemical engineering, 0210 nano-technology, Biofuel Cells, ComputingMilieux_MISCELLANEOUS
Abstract: After a short comparison of biofuel cells based on enzymes and microorganisms, several important developments and applications of enzymatic fuel cells (EFCs) are discussed. This discussion emphasizes how to evaluate the performance of EFCs, and highlights the influence of temperature and how it must be carefully considered for practical use of EFCs as power sources. Some of the latest and most important innovations in EFC design using buckypapers and redox nanoparticles are briefly reviewed.
Published: 2018
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38. Bioelectrode for detecting and/or oxidising glucose and method for the production thereof and device comprising same

Author: Giroud, Fabien, Gross, Andrew James, Cosnier, Serge, Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), Département de Chimie Moléculaire (DCM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), and Centre National de la Recherche Scientifique (CNRS)
Subjects: [CHIM]Chemical Sciences
Abstract: The invention concerns a bioelectrode and a device comprising same, for detecting or oxidising glucose. The bioelectrode of the invention comprises a layer of carbon nanotubes to which aromatic molecules are bonded, and FAD-GDH enzymes being adsorbed on the aromatic molecules. The invention applies to the field of biosensors and biofuel cells in particular.; L'invention concerne une bioélectrode et un dispositif la comprenant, pour la détection ou l'oxydation du glucose. La bioélectrode de l'invention comprend une couche de nanotubes de carbone auxquels des molécules aromatiques sont liées, et des enzymes FAD-GDH étant adsorbées aux molécules aromatiques. L'invention trouve application dans le domaine des biocapteurs et des biopiles à combustible, en particulier.
Published: 2018

39. Impedimetric quantification of anti-dengue antibodies using functional carbon nanotube deposits validated with blood plasma assays

Author: Quentin Palomar, Michael Holzinger, Serge Cosnier, Robert S. Marks, Chantal Gondran, Département de Chimie Moléculaire (DCM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Laboratoire d'électrochimie organique et de photochimie redox (LEOPR), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS), Ben-Gurion University of the Negev (BGU), Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), and Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])
Subjects: Chromatography, Chemistry, General Chemical Engineering, 010401 analytical chemistry, 02 engineering and technology, Carbon nanotube, Dengue virus, 021001 nanoscience & nanotechnology, medicine.disease_cause, medicine.disease, 01 natural sciences, 0104 chemical sciences, Dielectric spectroscopy, Dengue fever, law.invention, law, Covalent bond, Electrochemistry, medicine, Surface modification, [CHIM]Chemical Sciences, Cyclic voltammetry, 0210 nano-technology, Biosensor, ComputingMilieux_MISCELLANEOUS
Abstract: A high performance impedimetric immunosensor for the dengue virus antibody detection is presented. The setup profits from the formation of controlled and reproducible carbon nanotube (CNT) deposits on electrodes. Their easy functionalization via electrogeneration of a polypyrrole-NHS (N-hydroxysuccinimido 11-(pyrrol-1-yl) undecanoate) film enables the immobilization of the Dengue Virus 2 NS1 glycoprotein, the receptor unit, on the porous CNT layer via covalent amide coupling to provide the necessary selectivity towards Dengue NS1 antibody. All building steps of this immunosensor and the performance of this system were monitored by impedance spectroscopy and cyclic voltammetry. The resulting impedimetric dengue biosensor was tested in bovine blood plasma in addition to conventional measurements under controlled environment. After optimization, this immunosensor shows a good linearity in a wide concentration range (10−13–10−5 g mL−1).
Published: 2018
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40. Oriented Immobilization of [NiFeSe] Hydrogenases on Covalently and Noncovalently Functionalized Carbon Nanotubes for H 2 /Air Enzymatic Fuel Cells

Author: Christine Cavazza, Hélène Jamet, Alan Le Goff, Syamim Muhamad Che Mansor, Solène Gentil, Serge Cosnier, Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), Département de Chimie Moléculaire (DCM), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Département de Chimie Moléculaire - Chimie Inorganique Redox Biomimétique (DCM - CIRE), Institut de Chimie Moléculaire de Grenoble (ICMG)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Institut de Biosciences et de Biotechnologies de Grenoble (ex-IRTSV) (BIG), Institut National de la Santé et de la Recherche Médicale (INSERM)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Université Grenoble Alpes (UGA), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Laboratoire de Chimie et Biologie des Métaux (LCBM - UMR 5249), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques [2009-2015] (DCM - BEA [2009-2015]), Département de Chimie Moléculaire [2007-2015] (DCM [2007-2015]), Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), Département de Chimie Moléculaire - Chimie Inorganique Redox Biomimétique [2009-2015] (DCM - CIRE [2009-2015]), and Institut National de la Santé et de la Recherche Médicale (INSERM)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])
Subjects: Hydrogenase, hydrogen oxidation, 02 engineering and technology, Carbon nanotube, 010402 general chemistry, Electrochemistry, 01 natural sciences, Anthraquinone, Catalysis, law.invention, Hydrophobic effect, hydrogenases, chemistry.chemical_compound, law, [CHIM]Chemical Sciences, diazonium, ComputingMilieux_MISCELLANEOUS, carbon nanotubes, General Chemistry, Quartz crystal microbalance, biofuel cells, [CHIM.CATA]Chemical Sciences/Catalysis, [CHIM.MATE]Chemical Sciences/Material chemistry, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Chemical engineering, chemistry, Covalent bond, 0210 nano-technology
Abstract: International audience; We report the oriented immobilization of [NiFeSe] hydrogenases on both covalently and noncovalently modified carbon nanotubes (CNTs) electrodes. A specific interaction of the [NiFeSe] hydrogenase from Desulfomicrobium baculatum with hydrophobic organic molecules was probed by electrochemistry, quartz crystal microbalance with dissipation monitoring (QCM-D), and theoretical calculations. Taking advantage of these hydrophobic interactions, the enzyme was efficiently wired on anthraquinone and adamantane-modified CNTs. Because of rational immobilization onto functionalized CNTs, the O-2-tolerant [NiFeSe]-hydrogenase is able to efficiently operate in a H-2/air gas-diffusion enzymatic fuel cell.
Published: 2018
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41. Direct Electrochemistry of Bilirubin Oxidase from Magnaporthe orizae on Covalently-Functionalized MWCNT for the Design of High-Performance Oxygen-Reducing Biocathodes

Author: Solène Gentil, Marie Carrière, Serge Cosnier, Alan Le Goff, Sébastien Gounel, Nicolas Mano, Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), Département de Chimie Moléculaire (DCM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Vectorisation moléculaire et cellulaire, Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-RP Rorer-Centre National de la Recherche Scientifique (CNRS), Centre de recherches Paul Pascal (CRPP), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), ANR-11-LABX-0003,ARCANE,Grenoble, une chimie bio-motivée(2011), Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques [2016-2019] (DCM - BEA [2016-2019]), Département de Chimie Moléculaire [2016-2019] (DCM [2016-2019]), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Chimie Interface Biologie pour l’Environnement, la Santé et la Toxicologie [2017-2019] (CIBEST [2017-2019]), SYstèmes Moléculaires et nanoMatériaux pour l’Energie et la Santé (SYMMES), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Centre de Recherche Paul Pascal (CRPP), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), and ANR-11-LABX-0003/11-LABX-0003,ARCANE,Grenoble, une chimie bio-motivée(2011)
Subjects: Oxidoreductases Acting on CH-CH Group Donors, chemistry.chemical_element, 02 engineering and technology, 010402 general chemistry, Electrochemistry, 01 natural sciences, Oxygen, Catalysis, Diffusion, [CHIM]Chemical Sciences, Bilirubin oxidase, Hypoxia, Electrodes, ComputingMilieux_MISCELLANEOUS, biology, Organic Chemistry, General Chemistry, 021001 nanoscience & nanotechnology, biology.organism_classification, Combinatorial chemistry, 0104 chemical sciences, Nanostructures, Magnaporthe, chemistry, Covalent bond, Electrode, Surface modification, Myrothecium verrucaria, 0210 nano-technology
Abstract: Herein, the direct electrochemistry of bilirubin oxidase from Magnaporthe orizae (MoBOD) was studied on CNTs functionalized by electrografting several types of diazonium salts. The functionalization induces favorable or unfavorable orientation of MoBOD, the latter being compared to the well-known BOD from Myrothecium verrucaria (MvBOD). On the same nanostructured electrodes, MoBOD can surpass MvBOD in terms of both current densities and minimal overpotentials. Added to the fact that MoBOD is also highly active at the gas-diffusion electrode (GDE), these findings make MoBOD one of the MCOs with the highest catalytic activity towards the oxygen reduction reaction (ORR).
Published: 2018
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42. Comparison of Commercial and Lab-made MWCNT Buckypaper: Physicochemical Properties and Bioelectrocatalytic O 2 Reduction

Author: Fabien Giroud, Michael Holzinger, Serge Cosnier, Xiaohong Chen, Andrew J. Gross, Lardato, Marielle, Département de Chimie Moléculaire (DCM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Department of Neurology, the Third Affiliated Hospital, Sun Yat-Sen University, Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques [2016-2019] (DCM - BEA [2016-2019]), Département de Chimie Moléculaire [2016-2019] (DCM [2016-2019]), and Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])
Subjects: Chemistry, Buckypaper, O2 reduction, 02 engineering and technology, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, 3. Good health, 0104 chemical sciences, Analytical Chemistry, Chemical engineering, [CHIM] Chemical Sciences, Electrochemistry, [CHIM]Chemical Sciences, 0210 nano-technology, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2018
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43. Ferricyanide confined into the integrative system of pyrrolic surfactant and SWCNTs: The enhanced electrochemial sensing of paracetamol

Author: Xiao-Yan Wu, Xueji Zhang, Serge Cosnier, Shengyuan Deng, Dan Shan, Wen-Jun Niu, Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), Département de Chimie Moléculaire (DCM), Université Joseph Fourier - Grenoble 1 (UJF)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Université Joseph Fourier - Grenoble 1 (UJF)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Aqueous solution, Tetrafluoroborate, Chemistry, General Chemical Engineering, Inorganic chemistry, Carbon nanotube, Redox, Amperometry, law.invention, chemistry.chemical_compound, law, Electrochemistry, [CHIM]Chemical Sciences, Differential pulse voltammetry, Ferricyanide, Cyclic voltammetry, ComputingMilieux_MISCELLANEOUS
Abstract: An enhanced paracetamol sensor was developed based on the integrative system of single walled carbon nanotubes (SWCNTs) and the pyrrolic surfactant, (11-pyrrolyl-1-yl-undecyl)triethylammonium tetrafluoroborate (A 2 ), in which confined the redox probe, Fe(CN) 6 3−/4− . A well-defined redox peaks of the firmly confined Fe(CN) 6 3−/4− with dramatically negative shift in the half-wave potential compared to Fe(CN) 6 3−/4− in aqueous solution was observed at GCE/polyA 2 -Fe(CN) 6 3−/4− /SWCNTs. To understand the unusual Fe(CN) 6 3−/4− partitioning in this microheterogeneous system, the responses of the Fe(CN) 6 3−/4− at the electrodes modified by SWCNTs, polyA 2 , SWCNTs/polyA 2 , and polyA 2 /SWCNTs were investigated, respectively. The results of cyclic voltammetry (CV) and differential pulse voltammetry (DPV) indicated that the proposed electrode exhibiting efficient electrocatalytic capability towards the paracetamol oxidation. Thereafter, an amperometric assay for paracetamol at GCE/polyA 2 -Fe(CN) 6 3−/4− /SWCNTs was developed at 0.4 V with an enhanced linear range from 3.4×10 −7 ∼7.98 × 10 −4 M.
Published: 2015
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44. Biofunctionalizable flexible bucky paper by combination of multi-walled carbon nanotubes and polynorbornene-pyrene – Application to the bioelectrocatalytic reduction of oxygen

Author: Dafni Moatsou, Rachel K. O'Reilly, Raoudha Haddad, Serge Cosnier, Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), Département de Chimie Moléculaire (DCM), Université Joseph Fourier - Grenoble 1 (UJF)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Université Grenoble Alpes [2016-2019] (UGA [2016-2019])
Subjects: chemistry.chemical_classification, Materials science, Nanotechnology, Buckypaper, 02 engineering and technology, General Chemistry, Carbon nanotube, Polymer, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, 0104 chemical sciences, law.invention, chemistry.chemical_compound, Membrane, Adsorption, chemistry, law, Electrode, [CHIM]Chemical Sciences, Pyrene, General Materials Science, 0210 nano-technology, ComputingMilieux_MISCELLANEOUS, Filtration
Abstract: Owing to their conductivity, carbon nanotubes (CNTs) coatings are widely used for modifying electrode surfaces. In particular, the formation of bucky papers (BP) based on CNT assemblies obtained by filtration, represents an attractive way of creating a new kind of electrode. However, these BP are brittle and difficult to manipulate, hence their applications remain limited due to their fragility and lack of flexibility. Notably, their use for “in vivo” experiments is markedly hampered by the potential release of CNTs. The strengthening of the mechanical stability of BP has hereby been explored by the combination of BP and linear polymeric chains displaying non-covalent π-interactions with CNTs. These organic polymers act as a cross-linking agent throughout the BP assembly thus conferring stability and flexibility. Flexible BP electrodes were produced by simply mixing poly(norbornene) bearing pyrene groups and multi-walled CNTs, both dispersed in dimethylformamide followed by filtration through a PTFE membrane. The immobilization of laccase as a model protein was achieved on the resulting BP electrodes by adsorption or chemical grafting. These biocathodes displayed a direct electrical communication with laccase, allowing the reduction of oxygen in water (pH 5) with maximum current densities of ca 1.1 mA cm−2 at 0.4 V vs. SCE.
Published: 2015
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45. Synthesis and electrochemical characterization of original 'TEMPO' functionalized multiwall carbon nanotube materials: Application to iron (II) detection

Author: Karine Gorgy, Luisa-Roxana Popescu Mandoc, Kamal Elouarzaki, Cristina-Andreea Amarandei, Michael Holzinger, Serge Cosnier, Eleonora-Mihaela Ungureanu, MOLTECH-Anjou, Université d'Angers (UA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), Département de Chimie Moléculaire (DCM), and Université Joseph Fourier - Grenoble 1 (UJF)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Detection limit, Materials science, Stacking, Carbon nanotube, Electrochemistry, law.invention, Catalysis, Electrochemical gas sensor, lcsh:Chemistry, chemistry.chemical_compound, chemistry, Chemical engineering, lcsh:Industrial electrochemistry, lcsh:QD1-999, law, Molecule, Pyrene, Organic chemistry, [CHIM]Chemical Sciences, ComputingMilieux_MISCELLANEOUS, lcsh:TP250-261
Abstract: We report the synthesis and electrochemical behavior of an original catalyst bearing a 2,2,6,6-tetramethyl-1-piperidinyloxy (TEMPO) unit and a pyrene group. Anchoring of this bi-functional molecule by π -stacking onto multiwalled carbon nanotubes allows the study of the electrochemical properties of the compound in the immobilized state. These resulting functionalized TEMPO materials were used to detect iron(II) in solution with an experimental detection limit of 3.9 × 10−8 mol L−1 and a linear range between 7.8 × 10−8 and 1.4 × 10−6 mol L−1. Keywords: TEMPO, Multiwalled carbon nanotubes, Pyrene, Iron(II), Electrochemical sensor
Published: 2015
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46. Design of a reduced-graphene-oxide composite electrode from an electropolymerizable graphene aqueous dispersion using a cyclodextrin-pyrrole monomer. Application to dopamine biosensing

Author: Luminiţa Fritea, Alan Le Goff, Serge Cosnier, Jean-Luc Putaux, Cecilia Cristea, Mihaela Tertis, Robert Săndulescu, Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), Département de Chimie Moléculaire (DCM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Joseph Fourier - Grenoble 1 (UJF), Centre National de la Recherche Scientifique (CNRS), Iuliu Hatieganu University of Medicine and Pharmacy, Analytical Chemistry Department, and University of Medicine and Pharmacy
Subjects: Materials science, Aqueous solution, Nanocomposite, Graphene, General Chemical Engineering, Inorganic chemistry, Oxide, 02 engineering and technology, Glassy carbon, 010402 general chemistry, 021001 nanoscience & nanotechnology, Electrochemistry, 01 natural sciences, 0104 chemical sciences, law.invention, chemistry.chemical_compound, Monomer, chemistry, Chemical engineering, law, [CHIM]Chemical Sciences, 0210 nano-technology, ComputingMilieux_MISCELLANEOUS, Pyrrole
Abstract: A new cyclodextrin-modified pyrrole monomer (Py-CD) was synthesized and used both as an aqueous surfactant for the stabilization of aqueous reduced graphene oxide (RGO) dispersion and as an electropolymerizable unit to generate a RGO-polypyrrole composite electrode. This nanocomposite was used in combination with amphiphilic pyrrole derivative for the entrapment of tyrosinase, an enzyme catalyzing the oxidation of phenols and diphenols. Compared to glassy carbon electrodes, these nanostructured electrodes exhibit enhanced electroactive surface area and electrochemical properties and achieve higher performances towards catechol and dopamine biosensing.
Published: 2015
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47. A membraneless air-breathing hydrogen biofuel cell based on direct wiring of thermostable enzymes on carbon nanotube electrodes

Author: Pascale Infossi, Michael Holzinger, Sébastien Gounel, Raoudha Haddad, Anne de Poulpiquet, Michel Mermoux, Elisabeth Lojou, Noémie Lalaoui, Serge Cosnier, Nicolas Mano, Alan Le Goff, Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), Département de Chimie Moléculaire (DCM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Joseph Fourier - Grenoble 1 (UJF), Bioénergétique et Ingénierie des Protéines (BIP ), Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Joseph Fourier - Grenoble 1 (UJF), Centre de Recherche Paul Pascal (CRPP), Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Electrochimie et de Physico-chimie des Matériaux et des Interfaces (LEPMI ), Institut de Chimie du CNRS (INC)-Institut National Polytechnique de Grenoble (INPG)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Université Joseph Fourier - Grenoble 1 (UJF)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS), ANR-13-BIME-0003,CAROUCELL,Nanostructuration des anode et cathode pour une biopile à combustible H2/O2(2013), European Project: ANR-11-LABX-0003-01, European Project: ANR-12-BS08-0011-01, Université Joseph Fourier - Grenoble 1 (UJF)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Université Joseph Fourier - Grenoble 1 (UJF)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut National Polytechnique de Grenoble (INPG)-Institut de Chimie du CNRS (INC)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)
Subjects: Oxidoreductases Acting on CH-CH Group Donors, Materials science, Hydrogenase, Hydrogen, Bioelectric Energy Sources, Carbon nanotubes, chemistry.chemical_element, Bacillus, Nanotechnology, 02 engineering and technology, Carbon nanotube, 010402 general chemistry, 7. Clean energy, 01 natural sciences, Catalysis, law.invention, law, Materials Chemistry, Bilirubin oxidase, Electrodes, biology, Nanotubes, Carbon, Bacillus pumilus, Air, fungi, Metals and Alloys, Biofuel cell, [CHIM.CATA]Chemical Sciences/Catalysis, General Chemistry, Enzymes, Immobilized, 021001 nanoscience & nanotechnology, biology.organism_classification, Cathode, Enzymes, 0104 chemical sciences, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Anode, Oxygen, [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry, Chemical engineering, chemistry, Electrode, Ceramics and Composites, 0210 nano-technology, Oxidation-Reduction
Abstract: International audience; A biocathode was designed by the modification of a carbon nanotube (CNT) gas-diffusion electrode with bilirubin oxidase from Bacillus pumilus, achieving high current densities up to 3 mA cm−2 for the reduction of O2 from air. A membraneless air-breathing hydrogen biofuel cell was designed by combination of this cathode with a functionalized CNT-based hydrogenase anode.
Published: 2015
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48. Freestanding HRP–GOx redox buckypaper as an oxygen-reducing biocathode for biofuel cell applications

Author: Michael Holzinger, M. Bourourou, A. Le Goff, Robert S. Marks, Serge Cosnier, Kamal Elouarzaki, Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), Département de Chimie Moléculaire (DCM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Joseph Fourier - Grenoble 1 (UJF), Institute of Hydrology, and School of Materials Science & Engineering
Subjects: biology, Renewable Energy, Sustainability and the Environment, Chemistry, chemistry.chemical_element, Nanotechnology, Buckypaper, 7. Clean energy, Pollution, Oxygen, Redox, Horseradish peroxidase, Engineering::Materials::Energy materials [DRNTU], Catalysis, Electron transfer, chemistry.chemical_compound, Nuclear Energy and Engineering, Chemical engineering, biology.protein, [CHIM]Chemical Sciences, Environmental Chemistry, Glucose oxidase, Hydrogen peroxide, ComputingMilieux_MISCELLANEOUS
Abstract: Horseradish peroxidase (HRP) was immobilized on redox buckypapers followed by electropolymerization of pyrrole-modified concanavalin A enabling the subsequent additional immobilization of the glycoprotein glucose oxidase (GOx). Biocatalytic buckypapers were formed using pyrene-modified 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) or bis-Pyr-ABTS, a redox mediator, as a cross-linker. ABTS-functionalized buckypaper enhances the electron transfer of the bioelectrocatalytic reduction of H2O2 by HRP. Since H2O2 is produced during glucose oxidation by GOx in the presence of oxygen, the bienzymatic GOx–HRP biocathode achieves the complete reduction of oxygen into water. A clearly improved performance of the biocathode was obtained by using an improved biocompatible immobilization strategy, enabling the prevention of enzyme loss while ensuring both diffusion of glucose and O2 and the local production of H2O2. These freestanding flexible oxygen-reducing biocathodes can operate under physiological conditions and show a high onset potential at 0.60 (±0.01) V. In the presence of glucose (5 mM), such biocathodes exhibit a stable current density output of 1.1 (±0.1) mA cm−2 at 0.1 V under continuous one-hour discharge. Furthermore, a marked increase in lifetime was observed, the biocathode displaying 64% of its initial electrocatalytic activity after 15 days. Accepted version
Published: 2015
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49. Layer-by-layer scaffold formation using magnetic attraction between HiPCO® single-walled carbon nanotubes and magnetic nanoparticles: Application for high performance immunosensors

Author: Michael Holzinger, Maryam Tabrizian, Serge Cosnier, Meenakshi Singh, Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), Département de Chimie Moléculaire (DCM), Université Joseph Fourier - Grenoble 1 (UJF)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Department of Biomedical Engineering [Montréal] (BME), and McGill University = Université McGill [Montréal, Canada]
Subjects: chemistry.chemical_classification, Materials science, Layer by layer, Nanotechnology, General Chemistry, Polymer, Carbon nanotube, Amperometry, law.invention, chemistry, Impurity, law, [CHIM]Chemical Sciences, Magnetic nanoparticles, General Materials Science, Particle size, Biosensor, ComputingMilieux_MISCELLANEOUS
Abstract: Iron particle impurities of HiPCO® single-walled carbon nanotubes (SWCNTs) are treated as undesirable moieties and considerable times and efforts is allocated towards research and development to reduce their amount in HiPCO® SWCNTs. Taking advantage of this impurity, 3D nanostructured scaffolds are built via layer by layer (LBL) deposition of HiPCO® SWCNTs and magnetic nanoparticles which are retained via magnetic interaction with the iron particle impurities during the scaffold formation. The resulting scaffold has an inhomogeneous structure with large vacancies that can further be reinforced by electrogeneration of a functional polymer film to enable the immobilization of bioreceptor units for biosensing. Magnetic nanoparticles of different sizes are used to adjust the pore sizes within the scaffolds in order to determine the optimal particle size for their application as highly sensitive immunosensors. Scaffolds made of the magnetic nanoparticles with in average 500 nm sizes led to a sensitivity of 88 μA μg −1 mL cm −2 equivalent to a detection limit of 10 ng mL −1 for cholera antitoxin. This is by far the highest sensitivity for anti-CT immunosensors compared to amperometric transduction or ELISA.
Published: 2015
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50. Redox-Active Glyconanoparticles as Electron Shuttles for Mediated Electron Transfer with Bilirubin Oxidase in Solution

Author: Xiaohong Chen, Redouane Borsali, Andrew J. Gross, Fabien Giroud, Serge Cosnier, Christophe Travelet, Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), Département de Chimie Moléculaire (DCM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Centre de Recherches sur les Macromolécules Végétales (CERMAV ), and Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])
Subjects: Models, Molecular, Oxidoreductases Acting on CH-CH Group Donors, Nanoparticle, Electrons, Nanotechnology, Biosensing Techniques, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, 7. Clean energy, Biochemistry, Redox, Catalysis, Electron Transport, Electron transfer, Colloid and Surface Chemistry, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Molecule, [CHIM]Chemical Sciences, Benzothiazoles, Bilirubin oxidase, Electrodes, ComputingMilieux_MISCELLANEOUS, Cyclodextrins, Pyrenes, Aqueous solution, Chemistry, Electrochemical Techniques, General Chemistry, 021001 nanoscience & nanotechnology, Combinatorial chemistry, Electron transport chain, 0104 chemical sciences, Hypocreales, Nanoparticles, Sulfonic Acids, 0210 nano-technology, Oxidation-Reduction
Abstract: We demonstrate self-assembly, characterization and bioelectrocatalysis of redox-active cyclodextrin-coated nanoparticles. The nanoparticles with host–guest functionality are easy to assemble and permit entrapment of hydrophobic redox molecules in aqueous solution. Bis-pyrene-ABTS encapsulated nanoparticles were investigated electrochemically and spectroscopically. Their use as electron shuttles is demonstrated via an intraelectron transfer chain between neighboring redox units of clustered particles (Dh,DLS = 195 nm) and the mono- and trinuclear Cu sites of bilirubin oxidases. Enhanced current densities for mediated O2 reduction are observed with the redox nanoparticle system compared to equivalent bioelectrode cells with dissolved mediator. Improved catalytic stability over 2 days was also observed with the redox nanoparticles, highlighting a stabilizing effect of the polymeric architecture. Bioinspired nanoparticles as mediators for bioelectrocatalysis promises to be valuable for future biofuel cells an...
Published: 2017
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