1. Sustained release of TGF-β1 from biodegradable microparticles prepared by a new green process in CO2 medium
- Author
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Frank Boury, Pierre Weiss, Alison Dénarnaud, Jérôme Guicheux, Cécile Boyer, Thomas Cordonnier, Amin Swed, Micro et Nanomédecines Biomimétiques (MINT), and Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Bretagne Loire (UBL)
- Subjects
Transforming growth factor beta1 (TGF-b1) ,Protein encapsulation ,Cell Survival ,Scanning electron microscope ,Drug Compounding ,[SDV]Life Sciences [q-bio] ,Glycofurol ,Pharmaceutical Science ,Nanotechnology ,isosorbide dimethyl ether ,Isosorbide Dimethyl Ether ,Cell Line ,Transforming Growth Factor beta1 ,Injectable solvent ,chemistry.chemical_compound ,Polylactic Acid-Polyglycolic Acid Copolymer ,Zeta potential ,Animals ,Humans ,Lactic Acid ,sustained release ,Cytotoxicity ,Mice, Knockout ,chemistry.chemical_classification ,Chemistry ,Atomic force microscopy ,Green Chemistry Technology ,Polymer ,Carbon Dioxide ,Fibroblasts ,3. Good health ,Lactic acid ,Drug Liberation ,Chemical engineering ,Delayed-Action Preparations ,Polyglycolic Acid ,Transforming growth factor - Abstract
International audience; The aim of this work was to encapsulate transforming growth factor b1 (TGF-b1) into PLGA microparticles for regenerative medicine applications. TGF-b1 was firstly precipitated to ensure its stability during subsequent encapsulation within microparticles. A novel emulsification/extraction process in CO2 medium under mild conditions of pressure and temperature was used to encapsulate the protein. Interestingly, non-volatile injectable solvents, isosorbide dimethyl ether (DMI) and glycofurol (GF), were employed to precipitate the protein and to dissolve the polymer. Good encapsulation efficiency was obtained with preserved bioactivity of the protein. The microparticles were characterized in terms of size and zeta potential. In addition, the morphology and surface properties were determined using scanning electron microscopy (SEM) and atomic force microscopy (AFM) respectively. In vitro release study of the protein from microparticles was presented to assess the capacity of these systems to control the protein release. Moreover, cytotoxicity study was performed and showed an excellent cytocompatibility of the obtained microparticles. Thus, we described an effective and original process for TGF-b1 encapsulation into PLGA microparticles. The obtained polymeric carriers could be used in many biomedical applications and were more specifically developed for cartilage regeneration.
- Published
- 2015
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