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24 results on '"Dähnert, Lisa"'

2. Hepatitis E virus persists in the ejaculate of chronically infected men

Author

Horvatits, Thomas, Wißmann, Jan-Erik, Johne, Reimar, Groschup, Martin H., Gadicherla, Ashish K., Schulze zur Wiesch, Julian, Eiden, Martin, Todt, Daniel, Reimer, Rudolph, Dähnert, Lisa, Schöbel, Anja, Horvatits, Karoline, Lübke, Rabea, Wolschke, Christine, Ayuk, Francis, Rybczynski, Meike, Lohse, Ansgar W., Addo, Marylyn M., Herker, Eva, Lütgehetmann, Marc, Steinmann, Eike, and Pischke, Sven

Published
2021
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5. Hepatitis E virus: Efficacy of pasteurization of plasma‐derived VWF/FVIII concentrate determined by pig bioassay

Author

Dähnert, Lisa, Schlosser, Josephine, Fast, Christine, Fröhlich, Andreas, Gröner, Albrecht, Lange, Elke, Roth, Nathan J., Schäfer, Wolfram, Schröder, Charlotte, and Eiden, Martin

Subjects
pasteurization, infectivity, viruses, plasma product, 500 Naturwissenschaften und Mathematik::570 Biowissenschaften, Biologie::570 Biowissenschaften, Biologie, hepatitis E virus, inactivation
Abstract

Background Hepatitis E virus (HEV) is the leading cause of acute hepatitis throughout the world. Increasing blood component transfusion-associated HEV infections highlight the need for reliable virus inactivation procedures for plasma derivatives from pooled plasma donations. Study Design and Methods An animal infection study was conducted to evaluate the efficiency of HEV inactivation by pasteurization during the manufacturing process of the von Willebrand Factor/Factor VIII (VWF/FVIII) concentrate Haemate P/Humate-P (CSL Behring, Marburg, Germany). For this purpose, groups of pigs were inoculated with stabilized VWF/FVIII intermediate spiked with HEV-positive liver homogenate and exposed to increasing incubation times of 0, 3, 6, and 10 h at 60 degrees C. Animals were evaluated for virus replication over 27 days and in a subsequent trial over 92 days. Results Virus replication was detected in animals up to the 6-h pasteurization group. In contrast, pasteurization for 10 h did not reveal virus detection when the observation period was 27 days. In an additional experiment using the 10-h pasteurized material, two individuals started virus excretion and seroconverted when the observation period was extended to 92 days. Based on the total infection rate (2 of 12) of the animals inoculated with the sample pasteurized for 10 h, a virus reduction factor of at least 4.7 log(10) is calculated. Conclusion This study demonstrates that pasteurization at 60 degrees C for 10 h of an HEV-positive plasma derivative leads to the effective reduction of infectivity, resulting in a VWF/FVIII product with an appropriate margin of safety for HEV.

Published
2021

6. Hepatitis E virus: Efficacy of pasteurization of plasma‐derived VWF / FVIII concentrate determined by pig bioassay

Author

Dähnert, Lisa, primary, Schlosser, Josephine, additional, Fast, Christine, additional, Fröhlich, Andreas, additional, Gröner, Albrecht, additional, Lange, Elke, additional, Roth, Nathan J., additional, Schäfer, Wolfram, additional, Schröder, Charlotte, additional, Eiden, Martin, additional, and Groschup, Martin H., additional

Published
2021
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8. Hepatitis E virus shedding in semen of chronically, but not acute HEV infected individuals

Author

Horvatits, Thomas, primary, Groschup, Martin, additional, Grönmeyer, Johanna, additional, Eiden, Martin, additional, Dähnert, Lisa, additional, Rutter, Karoline, additional, Lübke, Rabea, additional, Ayuk, Francis, additional, Sabranski, Michael, additional, Rybczynski, Meike, additional, Lohse, Ansgar, additional, Addo, Marylyn, additional, Herker, Eva, additional, zur Wiesch, Julian Schulze, additional, Luetgehetmann, Marc, additional, and Pischke, Sven, additional

Published
2020
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16. Additional file 5: of High sensitivity of domestic pigs to intravenous infection with HEV

Author

Dähnert, Lisa, Eiden, Martin, Schlosser, Josephine, Fast, Christine, Schröder, Charlotte, Lange, Elke, Gröner, Albrecht, Schäfer, Wolfram, and Groschup, Martin

Subjects
3. Good health
Abstract

Alignment of the hypervariable region. Alignment done with Geneious version 10.2 created by Biomatters. Available from https://www.geneious.com (DOCX 244 kb)

17. Additional file 4: of High sensitivity of domestic pigs to intravenous infection with HEV

Author

Dähnert, Lisa, Eiden, Martin, Schlosser, Josephine, Fast, Christine, Schröder, Charlotte, Lange, Elke, Gröner, Albrecht, Schäfer, Wolfram, and Groschup, Martin

Subjects
viruses, virus diseases, digestive system diseases, 3. Good health
Abstract

Results of the RT-qPCR from serum and the porcine IgG HEV-Ab ELISA; The graphs display the individual curves of viral RNA in serum and the detection of Anti-HEV-IgG antibodies in correlation to days after infection (DOCX 462 kb)

18. Additional file 4: of High sensitivity of domestic pigs to intravenous infection with HEV

Author

Dähnert, Lisa, Eiden, Martin, Schlosser, Josephine, Fast, Christine, Schröder, Charlotte, Lange, Elke, Gröner, Albrecht, Schäfer, Wolfram, and Groschup, Martin

Subjects
viruses, virus diseases, digestive system diseases, 3. Good health
Abstract

Results of the RT-qPCR from serum and the porcine IgG HEV-Ab ELISA; The graphs display the individual curves of viral RNA in serum and the detection of Anti-HEV-IgG antibodies in correlation to days after infection (DOCX 462 kb)

19. Additional file 1: of High sensitivity of domestic pigs to intravenous infection with HEV

Author

Dähnert, Lisa, Eiden, Martin, Schlosser, Josephine, Fast, Christine, Schröder, Charlotte, Lange, Elke, Gröner, Albrecht, Schäfer, Wolfram, and Groschup, Martin

Subjects
viruses, virus diseases, digestive system diseases, 3. Good health
Abstract

Amplification curve of HEV specific qRT-PCR. A) Amplification curve of HEV specific qRT-PCR targeting the HEV standard (red line) and the RNA extracts from the inocula (blue line). B) Standard curves were obtained by Ct values plotted against the log of starting quantity. C) Obtained Ct values and determined copy numbers (DOCX 219 kb)

21. Additional file 1: of High sensitivity of domestic pigs to intravenous infection with HEV

Author

Dähnert, Lisa, Eiden, Martin, Schlosser, Josephine, Fast, Christine, Schröder, Charlotte, Lange, Elke, Gröner, Albrecht, Schäfer, Wolfram, and Groschup, Martin

Subjects
viruses, virus diseases, digestive system diseases, 3. Good health
Abstract

Amplification curve of HEV specific qRT-PCR. A) Amplification curve of HEV specific qRT-PCR targeting the HEV standard (red line) and the RNA extracts from the inocula (blue line). B) Standard curves were obtained by Ct values plotted against the log of starting quantity. C) Obtained Ct values and determined copy numbers (DOCX 219 kb)

22. Additional file 5: of High sensitivity of domestic pigs to intravenous infection with HEV

Author

Dähnert, Lisa, Eiden, Martin, Schlosser, Josephine, Fast, Christine, Schröder, Charlotte, Lange, Elke, Gröner, Albrecht, Schäfer, Wolfram, and Groschup, Martin

Subjects
3. Good health
Abstract

Alignment of the hypervariable region. Alignment done with Geneious version 10.2 created by Biomatters. Available from https://www.geneious.com (DOCX 244 kb)

23. Different Outcomes of Experimental Hepatitis E Virus Infection in Diverse Mouse Strains, Wistar Rats, and Rabbits.

Author

Schlosser, Josephine, Dähnert, Lisa, Dremsek, Paul, Tauscher, Kerstin, Fast, Christine, Ziegler, Ute, Gröner, Albrecht, Ulrich, Rainer G, Groschup, Martin H, and Eiden, Martin

Subjects
*HEPATITIS E virus, *LABORATORY rats, *LABORATORY rabbits, *IMMUNE response, *GENOTYPES
Abstract

Hepatitis E virus (HEV) is the causative agent of acute hepatitis E in humans in developing countries, but autochthonous cases of zoonotic genotype 3 (HEV-3) infection also occur in industrialized countries. In contrast to swine, rats, and rabbits, natural HEV infections in mice have not yet been demonstrated. The pig represents a well-established large animal model for HEV-3 infection, but a suitable small animal model mimicking natural HEV-3 infection is currently missing. Therefore, we experimentally inoculated C57BL/6 mice (wild-type, IFNAR−/−, CD4−/−, CD8−/−) and BALB/c nude (nu/nu) mice, Wistar rats, and European rabbits with a wild boar-derived HEV-3 strain and monitored virus replication and shedding, as well as humoral immune responses. HEV RNA and anti-HEV antibodies were detected in one and two out of eight of the rats and all rabbits inoculated, respectively, but not in any of the mouse strains tested. Remarkably, immunosuppressive dexamethasone treatment of rats did not enhance their susceptibility to HEV infection. In rabbits, immunization with recombinant HEV-3 and ratHEV capsid proteins induced protection against HEV-3 challenge. In conclusion, the rabbit model for HEV-3 infection may serve as a suitable alternative to the non-human primate and swine models, and as an appropriate basis for vaccine evaluation studies. [ABSTRACT FROM AUTHOR]

Published
2019
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24. Kinetics of maternally derived antibodies upon intramuscular vaccination against classical swine fever with Suvaxyn® CSF Marker (CP7_E2alf).

Author

Schröder, Charlotte, Dräger, Carolin, Aebischer, Andrea, Dähnert, Lisa, Breidenstein, Christiane, Mamerow, Svenja, Leidenberger, Simone, Beer, Martin, and Blome, Sandra

Subjects
*CLASSICAL swine fever vaccines, *IMMUNOGLOBULINS, *ANIMAL vaccination, *SWINE diseases
Abstract

Classical swine fever (CSF) remains one of the most important viral diseases that impact on sustainable pig production world wide. To control the disease in either endemic situations or in case of large, high-impact contingencies, safe and highly efficacious live attenuated vaccines exist since decades. However, until recently, the available live vaccines did not allow a serological marker concept that would be important to circumvent long-term trade restrictions. Recently, a new live attenuated marker vaccine, Suvaxyn ® CSF Marker (Zoetis), was licensed by the European Medicines Agency (EMA). To supplement the data that are necessary for the design of appropriate vaccination strategies, a trial was carried out with single “emergency-type” vaccination of two pregnant sows. Focus was laid on the kinetics of maternally derived antibodies (MDA) in the screening assays of their offspring that would be used in case of a CSF outbreaks, i.e. CSFV E2 and E rns antibody ELISA. Neutralization peroxidase linked antibody assays were carried out to allow a rough estimate of protection. Upon vaccination with Suvaxyn ® CSF Marker 21 days before farrowing, MDAs were measurable in all piglets born to the vaccinated sows. The E2 ELISA reactivities showed an almost linear decrease over 10 weeks after which all piglets were tested negative in the ELISA again. No problems were observed in DIVA assays (E rns antibodies) when heat-inactivated sera were used. The protective effect of MDA needs further investigations as the titers were found to be lower than reported for C-strain vaccines. [ABSTRACT FROM AUTHOR]

Published
2016
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