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5. The Effects of Seleno-Methionine in Cadmium-Challenged Human Primary Chondrocytes.

Author

Urzì Brancati, Valentina, Aliquò, Federica, Freni, José, Pantano, Alice, Galipò, Erika, Puzzolo, Domenico, Minutoli, Letteria, Marini, Herbert Ryan, Campo, Giuseppe Maurizio, and D'Ascola, Angela

Subjects
CELL physiology, PLANT-based diet, CELL survival, CARTILAGE cells, POLLUTION, CARTILAGE regeneration
Abstract

Cadmium (Cd) is a potentially toxic element able to interfere with cellular functions and lead to disease or even death. Cd accumulation has been demonstrated in cartilage, where it can induce damage in joints. The aim of this study was to evaluate the effect of CdCl2 on primary cultures of human chondrocytes and the possible protective effect of seleno-methionine (Se-Met). Human primary articular chondrocytes were cultured and treated as follows: control groups, cells challenged with 7.5 μM and 10 μM CdCl2 alone, and cells pretreated with 10 and 20 μM Se-Met and then challenged with 7.5 μM and 10 μM CdCl2. Twenty-four hours after incubation, cell viability, histological evaluation with hematoxylin–eosin stain, and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay were performed. Furthermore, reverse transcription-PCR was carried out to evaluate mRNA levels of BAX, BAK1, CASP-3, and CASP-9. After CdCl2 challenge at both doses, a reduced cell viability and an overexpression of BAX, BAK1, CASP-3, and CASP-9 genes, as well as a high number of TUNEL-positive cells, were demonstrated, all parameters becoming higher as the dose of CdCl2 was increased. The pretreatment with Se-Met lowered the expression of all considered genes, improved cell viability and morphological changes, and reduced the number of TUNEL-positive cells. It was concluded that Se-Met plays a protective role against CdCl2-induced structural and functional changes in chondrocytes in vitro, as it improved cell viability and showed a positive role in the context of the apoptotic pathways. It is therefore suggested that a translational, multifaceted approach, with plant-based diets, bioactive functional foods, nutraceuticals, micronutrients, and drugs, is possibly advisable in situations of environmental pollution caused by potentially toxic elements. [ABSTRACT FROM AUTHOR]

Published
2024
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16. Dynamics of astrocytes Ca2+ signaling: a low-cost fluorescence customized system for 2D cultures.

Author

Musotto, Rosa, Wanderlingh, Ulderico, D'Ascola, Angela, Spatuzza, Michela, Catania, Maria Vincenza, De Pittà, Maurizio, and Pioggia, Giovanni

Subjects
ASTROCYTES, FLUORESCENCE, FLUORESCENCE microscopy, CUSTOMIZATION, DATA analysis
Abstract

In an effort to help reduce the costs of fluorescence microscopy and expand the use of this valuable technique, we developed a low-cost platform capable of visualising and analysing the spatio-temporal dynamics of intracellular Ca2+ signalling in astrocytes. The created platform, consisting of a specially adapted fluorescence microscope and a data analysis procedure performed with Imagej Fiji software and custom scripts, allowed us to detect relative changes of intracellular Ca2+ ions in astrocytes. To demonstrate the usefulness of the workflow, we applied the methodology to several in vitro astrocyte preparations, specifically immortalised human astrocyte cells and wild-type mouse cells. To demonstrate the reliability of the procedure, analyses were conducted by stimulating astrocyte activity with the agonist dihydroxyphenylglycine (DHPG), alone or in the presence of the antagonist 2-methyl-6-phenylethyl-pyridine (MPEP). [ABSTRACT FROM AUTHOR]

Published
2024
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19. Polychlorinated biphenyls (PCBs) modulate AHR pathways leading to the altered expression of genes involved in extra-cellular matrix (ECM) remodeling and epigenetic regulation in thyrocytes

Author

Minuti, Aurelio, primary, Aliquo, Federica, additional, Giani, Fiorenza, additional, Lagana, Martina, additional, Ragonese, Marta, additional, Avenoso, Angela, additional, Cannavo, Salvatore, additional, Scuruchi, Michele, additional, D'Ascola, Angela, additional, and Ruggeri, Rosaria, additional

Published
2023
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22. Corrigendum: Exploiting curcumin synergy with natural products using quantitative analysis of dose-effect relationships in an experimental in vitro model of osteoarthritis

Author

D’Ascola, Angela, primary, Irrera, Natasha, additional, Ettari, Roberta, additional, Bitto, Alessandra, additional, Pallio, Giovanni, additional, Mannino, Federica, additional, Atteritano, Marco, additional, Campo, Giuseppe M., additional, Minutoli, Letteria, additional, Arcoraci, Vincenzo, additional, Squadrito, Violetta, additional, Picciolo, Giacomo, additional, Squadrito, Francesco, additional, and Altavilla, Domenica, additional

Published
2022
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30. Dioxin-like polychlorinated biphenyl (pcbs) congeners induce inflammatory responses and reduce thyroid-specific genes expression in human thyrocytes via ahr pathways

Author

Ruggeri, Rosaria, primary, Minuti, Aurelio, additional, Aliquo, Federica, additional, Giani, Fiorenza, additional, Masto, Roberta, additional, Romano, Davide, additional, Avenoso, Angela, additional, Teresa, Cristani Maria, additional, Trimarchi, Francesco, additional, Campenni, Alfredo, additional, D'Ascola, Angela, additional, and Cannavo, Salvatore, additional

Published
2022
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32. Catalytic Nanomedicine. Cu/TiO2–SiO2 Nanoparticles as Treatment of Diabetic Foot Ulcer: A Case Report

Author

Junqueira Virginia Berlanga Campos, Rahimi Nahid, Hashemi Mohammad, Alejandro Velázquez-Muñoz Javier, Liu Hui-Hui, M. Campo Giuseppe, Bahari Gholamreza, You Shou-jiang, Li Tianzhong, Murad Neif, D`Ascola Angela, López-Goerne Tessy, Mehdi Hashemi Seyed, Bacci Marcelo Rodrigues, Mandraffino Giuseppe, Reyes-González Jesús, Jin Xu, Azizi Mahsa, Qu Xinyuan, Chauhan Ritika, Zhang Chunyuan, Li Zheng, Yilin Guo, Saitta Antonino, Zatta Simone Meneghetti, Madhumalini M., Zhang Liping, Sun Hexu, Chauhan Vinita, Shi Jijun, Du Lei, Feder David, Cao Yong-jun, Avenoso Angela, Armando Pérez-Dávalos Luis, Kumar Dhaked Ram, Rocha Roberto Odebrecht, Sonkar Priyanka, Devi T. Meera, Fonseca Fernando Luiz Affonso, Adami Fernando, Alves Beatriz da Costa Aguiar, Yang Mengsu, Campo Salvatore, Ramírez-Olivares Paola, Bruschetta Giuseppe, Dong Weichao, Azzalis Ligia Ajaime, Chagas Antonio Carlos Palandri, and Scuruchi Michele

Subjects
Chemistry, Biomedical Engineering, Medicine (miscellaneous), Nanoparticle, Bioengineering, 02 engineering and technology, 021001 nanoscience & nanotechnology, medicine.disease, 030226 pharmacology & pharmacy, Catalysis, 03 medical and health sciences, 0302 clinical medicine, Diabetic foot ulcer, medicine, Nanomedicine, 0210 nano-technology, Nuclear chemistry
Abstract

Introduction: Diabetes mellitus is one of the most important health issues worldwide. The rising incidence of the disease has resulted in a parallel increase of complications such as diabetic foot ulcers (DFU). It is estimated that 25% of patients with diabetes will develop foot ulcers over a lifetime. In Mexico, this lifetime incidence is about 40% to 50%, and 20% of the cases will require amputations. DFU can result in staggering financial burdens for the healthcare system and the patient. Therapies that promote rapid and complete healing and reduce the need for expensive surgical procedures would impact these costs substantially. Methods: Cu/TiO2–SiO2 nanoparticles were synthesized by T. López et al. sol-gel patented method. After synthesis, they were characterized using transmission electron microscopy (TEM) and X-ray diffraction. Then, they were embedded in a polymeric gel matrix. The Cu/TiO2–SiO2 nanogel was used as conservative therapy for a chronic non-healing DFU on a 62-year old female with several comorbidities and chronic complications of diabetes. Wound debridement was performed prior to nanogel administration. The nanogel was applied over the ulcer on alternate days for an initial period of 2 weeks and then continued for 10 months. Results: Significant improvement was observed in the wound healing process since the first applications. The infection was limited and tissue regeneration was enhanced until complete healing of the ulcer. Conclusion: Cu/TiO2–SiO2 nanogel therapy enhanced reepithelialization and healing of the DFU. The successful outcome allowed to avoid the amputation that was proposed for the patient.

Published
2020
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35. Expression and Change of miRs 145, 221 and 222 in Hypertensive Subjects Treated with Enalapril, Losartan or Olmesartan

Author

Mandraffino, Giuseppe, primary, Lo Gullo, Alberto, additional, Cinquegrani, Maria, additional, D’Ascola, Angela, additional, Sinicropi, Davide, additional, Imbalzano, Egidio, additional, Blando, Giuseppe, additional, Campo, Giuseppe Maurizio, additional, Morace, Carmela, additional, Giuffrida, Clemente, additional, Campo, Salvatore, additional, Squadrito, Giovanni, additional, and Scuruchi, Michele, additional

Published
2021
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48. Altered Long Noncoding RNA Expression Profile in Multiple Myeloma Patients with Bisphosphonate-Induced Osteonecrosis of the Jaw

Author

Allegra, Alessandro, primary, Mania, Manuela, additional, D’Ascola, Angela, additional, Oteri, Giacomo, additional, Siniscalchi, Enrico Nastro, additional, Avenoso, Angela, additional, Innao, Vanessa, additional, Scuruchi, Michele, additional, Allegra, Andrea Gaetano, additional, Musolino, Caterina, additional, and Campo, Salvatore, additional

Published
2020
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49. β-Caryophyllene Inhibits Cell Proliferation through a Direct Modulation of CB2 Receptors in Glioblastoma Cells

Author

Irrera, Natasha, primary, D’Ascola, Angela, additional, Pallio, Giovanni, additional, Bitto, Alessandra, additional, Mannino, Federica, additional, Arcoraci, Vincenzo, additional, Rottura, Michelangelo, additional, Ieni, Antonio, additional, Minutoli, Letteria, additional, Metro, Daniela, additional, Vaccaro, Mario, additional, Altavilla, Domenica, additional, and Squadrito, Francesco, additional

Published
2020
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