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1. NFKBIE mutations are selected by the tumor microenvironment and contribute to immune escape in chronic lymphocytic leukemia

Author

Bonato, Alice, Chakraborty, Supriya, Bomben, Riccardo, Canarutto, Giulia, Felician, Giulia, Martines, Claudio, Zucchetto, Antonella, Pozzo, Federico, Vujovikj, Marija, Polesel, Jerry, Chiarenza, Annalisa, Del Principe, Maria Ilaria, Del Poeta, Giovanni, D’Arena, Giovanni, Marasca, Roberto, Tafuri, Agostino, Laurenti, Luca, Piazza, Silvano, Dimovski, Aleksandar J., Gattei, Valter, and Efremov, Dimitar G.

Published
2024
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3. Cognitive Health of Nonagenarians in Southern Italy: A Descriptive Analysis from a Cross-Sectional, Home-Based Pilot Study of Exceptional Longevity (Cilento Initiative on Aging Outcomes Or CIAO).

Author

Pizza, Vincenzo, Antonini, Paola, Marino, Rosella, D'Arena, Giovanni, Lucibello, Serena Grazia, Rizzo, Marianna, Brenner, David A, Jeste, Dilip V, and Somma, Salvatore Di

Subjects
Humans, Cross-Sectional Studies, Pilot Projects, Neuropsychological Tests, Aging, Longevity, Aged, 80 and over, Home Care Services, Italy, Female, Male, Cognitive Dysfunction, Cilento Region, cognitive health, lifestyle, longevity, General & Internal Medicine
Abstract

Background: Nonagenarians and centenarians (NCs) are an extremely fragile population, particularly in regard to their physical and cognitive function. The aim of this study was to define the neurocognitive profiles among 29 NCs and their 49 younger cohabitants aged 50-75 years from The Cilento Initiative on Aging Outcomes (CIAO) Pilot study in the South of Italy that had provided initial hypotheses regarding positive psychological traits related to exceptional longevity. Methods: During the home visits, lifestyle information with specific questionnaires, functional autonomy and the neuropsychological Mini Mental Scale Examination (MMSE), and the Alzheimer's Disease Assessment Scale-Cognitive (ADAS-Cog) scale were obtained by qualified study personnel. The total blood oxidative capacity was also determined by testing the reactive derivative of oxygen metabolites (d-ROM) and by the Biological Antioxidant Potential (BAP). In all individuals, the APOE genotype determination was also performed. Results: All the subjects in both groups showed high adherence to the Mediterranean Diet. None of the NCs had severe cognitive impairment, and a very low incidence of dementia was found. The data obtained on the Activities ed Instrumental Activities of Daily Living (ADL-IADL) scale showed that the majority of NCs (16/29) were autonomous in daily life activities. The comparative assessment of NCs and cohabitants showed no significant differences in the laboratory assessment of oxidative stress and APOE genotype. Conclusion: In the Cilento Region of Southern Italy, NCs seemed to have good cognitive status when compared to younger cohabitants aging 50-65 years without significant differences in oxidative stress markers or APOE genotype. These results might be related to optimal adherence to the Mediterranean diet, although other lifestyle factors and positive personality traits may also contribute to their healthy aging. Further studies on a larger population should be performed to confirm the results of this pilot study.

Published
2020

4. What Does Atypical Chronic Lymphocytic Leukemia Really Mean? A Retrospective Morphological and Immunophenotypic Study

Author

D’Arena, Giovanni, primary, Vitale, Candida, additional, Pietrantuono, Giuseppe, additional, Villani, Oreste, additional, Mansueto, Giovanna, additional, D’Auria, Fiorella, additional, Statuto, Teodora, additional, D’Agostino, Simona, additional, Sabetta, Rosalaura, additional, Tarasco, Angela, additional, Innocenti, Idanna, additional, Autore, Francesco, additional, Fresa, Alberto, additional, Valvano, Luciana, additional, Tomasso, Annamaria, additional, Cafaro, Lorenzo, additional, Lamorte, Daniela, additional, and Laurenti, Luca, additional

Published
2024
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7. T-Large Granular Lymphocytic Leukemia with Hepatosplenic T-Cell Lymphoma? A Rare Case of Simultaneous Neoplastic T-Cell Clones Highlighted by Flow Cytometry and Review of Literature.

Author

Libonati, Rossana, Soda, Michela, Statuto, Teodora, Valvano, Luciana, D'Auria, Fiorella, D'Arena, Giovanni, Pietrantuono, Giuseppe, Villani, Oreste, Mansueto, Giovanna Rosaria, D'Agostino, Simona, Di Somma, Massimo Dante, Telesca, Alessia, and Vilella, Rocchina

Subjects
LYMPHOCYTIC leukemia, T-cell lymphoma, LITERATURE reviews, FLOW cytometry, MOLECULAR cloning
Abstract

Lymphoproliferative diseases are a heterogeneous set of malignant clonal proliferations of lymphocytes. Despite well-established diagnostic criteria, the diagnosis remains difficult due to their variety in clinical presentation and immunophenotypic profile. Lymphoid T-cell disorders are less common than B-cell entities, and the lack of a clear immunophenotypic characteristic makes their identification hard. Flow cytometry turned out to be a useful tool in diagnosing T-cell disorders and to resolve complicated cases, especially if the number of analyzable neoplastic cells is small. We present a case of a 55-year-old man with simultaneous lymphoproliferative neoplastic T-cell clones, one αβ and the other γδ, identified and characterized by flow cytometry (FC), exploiting the variable expression intensity of specific markers. However, the patient's rapid decline made it impossible to define a differential diagnosis in order to confirm the identity of the γδ clone, which remains uncertain. This case is added to the few other cases already documented in the literature, characterized by the co-existence of T-large granular lymphocytic leukemia (T-LGLL)-αβ and T-LGLL-γδ/Hepatosplenic T-cell lymphoma (HSTCL). Our case underlines the key role of sensitive diagnostic tools in the assessment of potential relationship between the diagnosis, prognosis, and treatment in the two pathologies. [ABSTRACT FROM AUTHOR]

Published
2024
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9. Internalization of Pegylated Er:Y2O3 Nanoparticles inside HCT-116 Cancer Cells: Implications for Imaging and Drug Delivery

Author

Chiechio, Regina Maria, primary, Caponnetto, Angela, additional, Battaglia, Rosalia, additional, Ferrara, Carmen, additional, Butera, Ester, additional, Musumeci, Paolo, additional, Reitano, Riccardo, additional, Ruffino, Francesco, additional, Maccarrone, Giuseppe, additional, Di Pietro, Cinzia, additional, Marchi, Valérie, additional, Lanzanò, Luca, additional, Arena, Giovanni, additional, Grasso, Alfina, additional, Copat, Chiara, additional, Ferrante, Margherita, additional, and Contino, Annalinda, additional

Published
2023
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11. What Does Atypical Chronic Lymphocytic Leukemia Really Mean? A Retrospective Morphological and Immunophenotypic Study.

Author

D'Arena, Giovanni, Vitale, Candida, Pietrantuono, Giuseppe, Villani, Oreste, Mansueto, Giovanna, D'Auria, Fiorella, Statuto, Teodora, D'Agostino, Simona, Sabetta, Rosalaura, Tarasco, Angela, Innocenti, Idanna, Autore, Francesco, Fresa, Alberto, Valvano, Luciana, Tomasso, Annamaria, Cafaro, Lorenzo, Lamorte, Daniela, and Laurenti, Luca

Subjects
*CHRONIC lymphocytic leukemia, *FLOW cytometry, *RETROSPECTIVE studies, *ACQUISITION of data, *CANCER patients, *GENE expression, *SYMPTOMS, *MEDICAL records, *IMMUNOPHENOTYPING, *TUMOR markers, *PHENOTYPES
Abstract

Simple Summary: Chronic lymphocytic leukemia (CLL) may be atypical in terms of the cell morphology picture, but also with regard to the surface immunophenotypic profile. Aiming at assessing the impact of morphology and immunophenotype in defining the atypical characteristics of CLL in terms of clinical–biological features and prognosis, a retrospective analysis of a large cohort of CLL patients was performed. We found that morphology better predicts the prognosis of atypical CLL compared to immunophenotypic analysis. Also, discordant cases in terms of immunophenotype and morphology did not identify specific prognostic groups. Overall, the question that still needs to be answered is: does it make sense to focus on morphology and immunophenotypic features in CLL in the era of molecular markers used as prognostic indicators? Atypical chronic lymphocytic leukemia (CLL) is still defined according to morphological criteria. However, deviance from the typical surface immunological profile suggests an atypical immunological-based CLL. A large cohort of patients with CLL was retrospectively evaluated aiming at assessing morphological (FAB criteria), immunophenotypical (two or more discordances from the typical profile), and clinical–biological features of atypical CLL. Compared to typical cases, morphologically atypical CLL showed a greater percentage of unmutated IgVH and CD38 positivity, and a higher expression of CD20. Immunophenotypically atypical CLL was characterized by more advanced clinical stages, higher expression of CD20, higher rate of FMC7, CD79b and CD49d positivity, and by an intermediate–high expression of membrane surface immunoglobulin, compared to typical cases. When patients were categorized based on immunophenotypic and morphologic concordance or discordance, no difference emerged. Finally, morphological features better discriminated patients' prognosis in terms of time-to-first treatment, while concordant atypical cases showed overall a worse prognosis. Discordant cases by immunophenotype and/or morphology did not identify specific prognostic groups. Whether—in the era of molecular markers used as prognostic indicators—it does make sense to focus on morphology and immunophenotype features in CLL is still matter of debate needing further research. [ABSTRACT FROM AUTHOR]

Published
2024
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12. XPO1 mutations identify early‐stage CLL characterized by shorter time to first treatment and enhanced BCR signalling

Author

Moia, Riccardo, primary, Terzi di Bergamo, Lodovico, additional, Talotta, Donatella, additional, Bomben, Riccardo, additional, Forestieri, Gabriela, additional, Spina, Valeria, additional, Bruscaggin, Alessio, additional, Cosentino, Chiara, additional, Almasri, Mohammad, additional, Dondolin, Riccardo, additional, Bittolo, Tamara, additional, Zucchetto, Antonella, additional, Baldoni, Stefano, additional, Del Giudice, Ilaria, additional, Mauro, Francesca Romana, additional, Maffei, Rossana, additional, Chiarenza, Annalisa, additional, Tafuri, Agostino, additional, Laureana, Roberta, additional, Del Principe, Maria Ilaria, additional, Zaja, Francesco, additional, D'Arena, Giovanni, additional, Olivieri, Jacopo, additional, Rasi, Silvia, additional, Mahmoud, Abdurraouf, additional, Al Essa, Wael, additional, Awikeh, Bassel, additional, Kogila, Sreekar, additional, Bellia, Matteo, additional, Mouhssine, Samir, additional, Sportoletti, Paolo, additional, Marasca, Roberto, additional, Scarfò, Lydia, additional, Ghia, Paolo, additional, Gattei, Valter, additional, Foà, Robin, additional, Rossi, Davide, additional, and Gaidano, Gianluca, additional

Published
2023
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13. P606: MEASUREMENT OF INTRACLONAL DIVERSIFICATION REFINES THE PROGNOSTIC IMPACT OF IGHV MUTATIONS IN CLL

Author

Vit, Filippo, primary, Bittolo, Tamara, additional, Papotti, Robel, additional, Pozzo, Federico, additional, Zucchetto, Antonella, additional, Tissino, Erika, additional, Zaina, Eva, additional, Catarossi, Ilaria, additional, Laureana, Roberta, additional, Olivieri, Jacopo, additional, Bulian, Pietro, additional, Levato, Luciano, additional, D’arena, Giovanni, additional, Armiento, Daniele, additional, Zamò, Alberto, additional, Leich, Ellen, additional, Rosenwald, Andreas, additional, Arons, Evgeny, additional, J. Kreitman, Robert, additional, Gentile, Massimo, additional, Laurenti, Luca, additional, Zaja, Francesco, additional, Tafuri, Agostino, additional, Chiarenza, Annalisa, additional, Ilaria DEL Principe, Maria, additional, Gattei, Valter, additional, and Bomben, Riccardo, additional

Published
2023
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14. P597: XPO1 MUTATIONS IDENTIFY EARLY STAGE CLL CHARACTERIZED BY SHORTER TIME TO FIRST TREATMENT AND ENHANCED BCR SIGNALING

Author

Talotta, Donatella, primary, Moia, Riccardo, additional, Terzi DI Bergamo, Lodovico, additional, Bomben, Riccardo, additional, Forestieri, Gabriela, additional, Spina, Valeria, additional, Bruscaggin, Alessio, additional, Cosentino, Chiara, additional, Almasri, Mohammad, additional, Dondolin, Riccardo, additional, Bittolo, Tamara, additional, Zucchetto, Antonella, additional, Baldoni, Stefano, additional, Del Giudice, Ilaria, additional, Romana Mauro, Francesca, additional, Maffei, Rossana, additional, Chiarenza, Annalisa, additional, Tafuri, Agostino, additional, Laureana, Roberta, additional, Ilaria Del Principe, Maria, additional, Zaja, Francesco, additional, D’arena, Giovanni, additional, Olivieri, Jacopo, additional, Rasi, Silvia, additional, Mahmoud, Abdurraouf, additional, Al Essa, Wael, additional, Awikeh, Bassel, additional, Kogila, Sreekar, additional, Bellia, Matteo, additional, Mouhssine, Samir, additional, Sportoletti, Paolo, additional, Marasca, Roberto, additional, Scarfò, Lydia, additional, Ghia, Paolo, additional, Gattei, Valter, additional, Foà, Robin, additional, Rossi, Davide, additional, and Gaidano, Gianluca, additional

Published
2023
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15. Is re-challenge still an option as salvage therapy in multiple myeloma? The case of REal-life BOrtezomib re-Use as secoND treatment for relapsed patients exposed frontline to bortezomib-based therapies (the REBOUND Study)

Author

Musto, Pellegrino, Simeon, Vittorio, Cascavilla, Nicola, Falcone, Antonietta, Petrucci, Maria Teresa, Cesini, Laura, Di Raimondo, Francesco, Conticello, Concetta, Ria, Roberto, Catalano, Lucio, Salvatore, Dalila, Mastrullo, Lucia, Gagliardi, Alfredo, Villani, Oreste, Pietrantuono, Giuseppe, D’Arena, Giovanni, Mansueto, Giovanna, Bringhen, Sara, Genuardi, Mariella, Di Renzo, Nicola, Reddiconto, Giovanni, Fragasso, Alberto, Caravita, Tommaso, Scapicchio, Daniele, Marziano, Gioacchino, Boccadoro, Mario, Mangiacavalli, Silvia, and Corso, Alessandro

Published
2019
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16. Preliminary analysis of double‐negative T, double‐positive T, and natural killer T‐like cells in B‐cell chronic lymphocytic leukemia

Author

Valvano, Luciana, primary, Nozza, Filomena, additional, D'Arena, Giovanni, additional, D'Auria, Fiorella, additional, De Luca, Luciana, additional, Pietrantuono, Giuseppe, additional, Mansueto, Giovanna, additional, Villani, Oreste, additional, D'Agostino, Simona, additional, Lamorte, Daniela, additional, Calice, Giovanni, additional, and Statuto, Teodora, additional

Published
2023
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17. The VLA-4 integrin is constitutively active in circulating chronic lymphocytic leukemia cells via BCR autonomous signaling: a novel anchor-independent mechanism exploiting soluble blood-borne ligands

Author

Tissino, Erika, Gaglio, Annalisa, Nicolò, Antonella, Pozzo, Federico, Bittolo, Tamara, Rossi, Francesca Maria, Bomben, Riccardo, Nanni, Paola, Cattarossi, Ilaria, Zaina, Eva, Zimbo, Anna Maria, Ianna, Giulia, Capasso, Guido, Forestieri, Gabriela, Moia, Riccardo, Datta, Moumita, Härzschel, Andrea, Olivieri, Jacopo, D’Arena, Giovanni, Laurenti, Luca, Zaja, Francesco, Chiarenza, Annalisa, Palumbo, Giuseppe A., Martino, Enrica Antonia, Gentile, Massimo, Rossi, Davide, Gaidano, Gianluca, Del Poeta, Giovanni, Laureana, Roberta, Del Principe, Maria Ilaria, Maity, Palash C., Jumaa, Hassan, Hartmann, Tanja Nicole, Zucchetto, Antonella, and Gattei, Valter

Abstract

In chronic lymphocytic leukemia (CLL), survival of neoplastic cells depends on microenvironmental signals at lymphoid sites where the crosstalk between the integrin VLA-4 (CD49d/CD29), expressed in ~40% of CLL, and the B-cell receptor (BCR) occurs. Here, BCR engagement inside–out activates VLA-4, thus enhancing VLA-4-mediated adhesion of CLL cells, which in turn obtain pro-survival signals from the surrounding microenvironment. We report that the BCR is also able to effectively inside–out activate the VLA-4 integrin in circulating CD49d-expressing CLL cells through an autonomous antigen-independent BCR signaling. As a consequence, circulating CLL cells exhibiting activated VLA-4 express markers of BCR pathway activation (phospho-BTK and phospho-PLC-γ2) along with higher levels of phospho-ERK and phospho-AKT indicating parallel activation of downstream pathways. Moreover, circulating CLL cells expressing activated VLA-4 bind soluble blood-borne VCAM-1 leading to increased VLA-4-dependent actin polymerization/re-organization and ERK phosphorylation. Finally, evidence is provided that ibrutinib treatment, by affecting autonomous BCR signaling, impairs the constitutive VLA-4 activation eventually decreasing soluble VCAM-1 binding and reducing downstream ERK phosphorylation by circulating CLL cells. This study describes a novel anchor-independent mechanism occurring in circulating CLL cells involving the BCR and the VLA-4 integrin, which help to unravel the peculiar biological and clinical features of CD49d+ CLL.

Published
2024
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18. Internalization of Pegylated Er:Y2O3 Nanoparticles inside HCT-116 Cancer Cells: Implications for Imaging and Drug Delivery.

Author

Chiechio, Regina Maria, Caponnetto, Angela, Battaglia, Rosalia, Ferrara, Carmen, Butera, Ester, Musumeci, Paolo, Reitano, Riccardo, Ruffino, Francesco, Maccarrone, Giuseppe, Di Pietro, Cinzia, Marchi, Valérie, Lanzanò, Luca, Arena, Giovanni, Grasso, Alfina, Copat, Chiara, Ferrante, Margherita, and Contino, Annalinda

Abstract

Lanthanide-doped nanoparticles, featuring sharp emission peaks with narrow bandwidth, exhibit high downconversion luminescence intensity, making them highly valuable in the fields of bioimaging and drug delivery. High-crystallinity Y2O3 nanoparticles (NPs) doped with Er3+ ions were functionalized by using a pegylation procedure to confer water solubility and biocompatibility. The NPs were thoroughly characterized using transmission electron microscopy (TEM), inductively coupled plasma mass spectrometry (ICP-MS), and photoluminescence measurements. The pegylated nanoparticles were studied both from a toxicological perspective and to demonstrate their internalization within HCT-116 cancer cells. Cell viability tests allowed for the identification of the "optimal" concentration, which yields a detectable fluorescence signal without being toxic to the cells. The internalization process was investigated using a combined approach involving confocal microscopy and ICP-MS. The obtained data clearly indicate the efficient internalization of NPs into the cells with emission intensity showing a strong correlation with the concentrations of nanoparticles delivered to the cells. Overall, this research contributes significantly to the fields of nanotechnology and biomedical research, with noteworthy implications for imaging and drug delivery applications. [ABSTRACT FROM AUTHOR]

Published
2023
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19. Supplementary Data from TP53 Mutations with Low Variant Allele Frequency Predict Short Survival in Chronic Lymphocytic Leukemia

Author

Bomben, Riccardo, primary, Rossi, Francesca Maria, primary, Vit, Filippo, primary, Bittolo, Tamara, primary, D'Agaro, Tiziana, primary, Zucchetto, Antonella, primary, Tissino, Erika, primary, Pozzo, Federico, primary, Vendramini, Elena, primary, Degan, Massimo, primary, Zaina, Eva, primary, Cattarossi, Ilaria, primary, Varaschin, Paola, primary, Nanni, Paola, primary, Berton, Michele, primary, Braida, Alessandra, primary, Polesel, Jerry, primary, Cohen, Jared A., primary, Santinelli, Enrico, primary, Biagi, Annalisa, primary, Gentile, Massimo, primary, Morabito, Fortunato, primary, Fronza, Gilberto, primary, Pozzato, Gabriele, primary, D'Arena, Giovanni, primary, Olivieri, Jacopo, primary, Bulian, Pietro, primary, Pepper, Chris, primary, Hockaday, Anna, primary, Schuh, Anna, primary, Hillmen, Peter, primary, Rossi, Davide, primary, Chiarenza, Annalisa, primary, Zaja, Francesco, primary, Di Raimondo, Francesco, primary, Del Poeta, Giovanni, primary, and Gattei, Valter, primary

Published
2023
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21. Supplementary Table 1, Figures 1-4 from Nicotinamide Blocks Proliferation and Induces Apoptosis of Chronic Lymphocytic Leukemia Cells through Activation of the p53/miR-34a/SIRT1 Tumor Suppressor Network

Author

Audrito, Valentina, primary, Vaisitti, Tiziana, primary, Rossi, Davide, primary, Gottardi, Daniela, primary, D'Arena, Giovanni, primary, Laurenti, Luca, primary, Gaidano, Gianluca, primary, Malavasi, Fabio, primary, and Deaglio, Silvia, primary

Published
2023
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23. The immunomodulatory molecule TIGIT is expressed by chronic lymphocytic leukemia cells and contributes to anergy

Author

Arruga, Francesca, primary, Rubin, Marta, additional, Papazoglou, Despoina, additional, Iannello, Andrea, additional, Ioannou, Nikolaos, additional, Moia, Riccardo, additional, Rossi, Davide, additional, Gaidano, Gianluca, additional, Coscia, Marta, additional, Laurenti, Luca, additional, D’Arena, Giovanni, additional, Allan, John N., additional, Furman, Richard R., additional, Vaisitti, Tiziana, additional, Ramsay, Alan G., additional, and Deaglio, Silvia, additional

Published
2023
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25. Bendamustine in combination with rituximab for elderly patients with previously untreated B-cell chronic lymphocytic leukemia: A retrospective analysis of real-life practice in Italian hematology departments

Author

Laurenti, Luca, Innocenti, Idanna, Autore, Francesco, Vannata, Barbara, Efremov, Dimitar G., Ciolli, Stefania, Del Poeta, Giovanni, Mauro, Francesca Romana, Cortelezzi, Agostino, Borza, Paola Anticoli, Ghio, Francesco, Mondello, Patrizia, Murru, Roberta, Gozzetti, Alessandro, Cariccio, Maria Rosa Lanza, Piccirillo, Nicola, Boncompagni, Riccardo, Cantonetti, Maria, Principe, Maria Ilaria Del, Reda, Gianluigi, Bongarzoni, Velia, Cervetti, Giulia, Pitini, Vincenzo, Foà, Robin, Sica, Simona, and D’Arena, Giovanni

Published
2015
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28. An Unsupervised Machine Learning Method Stratifies Chronic Lymphocytic Leukemia Patients in Novel Categories with Different Risk of Early Treatment

Author

Cuturello, Francesca, primary, Pozzo, Federico, additional, Villegas Garcia, Edith Natalia, additional, Rossi, Francesca Maria, additional, Degan, Massimo, additional, Nanni, Paola, additional, Cattarossi, Ilaria, additional, Zaina, Eva, additional, Varaschin, Paola, additional, Braida, Alessandra, additional, Berton, Michele, additional, Zannier, Laura, additional, Vit, Filippo, additional, Tissino, Erika, additional, Bittolo, Tamara, additional, Laureana, Roberta, additional, D'Arena, Giovanni, additional, Laurenti, Luca, additional, Tafuri, Agostino, additional, Olivieri, Jacopo, additional, Zaja, Francesco, additional, Chiarenza, Annalisa, additional, Del Principe, Maria Ilaria, additional, Bomben, Riccardo, additional, Zucchetto, Antonella, additional, Cozzini, Stefano, additional, Ansuini, Alessio, additional, Cazzaniga, Alberto, additional, and Gattei, Valter, additional

Published
2022
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29. MicroRNA-155 in serum-derived extracellular vesicles as a potential biomarker for hematologic malignancies - a short report

Author

Caivano, Antonella, La Rocca, Francesco, Simeon, Vittorio, Girasole, Marco, Dinarelli, Simone, Laurenzana, Ilaria, De Stradis, Angelo, De Luca, Luciana, Trino, Stefania, Traficante, Antonio, D’Arena, Giovanni, Mansueto, Giovanna, Villani, Oreste, Pietrantuono, Giuseppe, Laurenti, Luca, Del Vecchio, Luigi, and Musto, Pellegrino

Published
2017
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31. A Multi-Parametric and Multi-Layer Study to Investigate the Largest 2022 Hunga Tonga–Hunga Ha’apai Eruptions

Author

D’Arcangelo, Serena, primary, Bonforte, Alessandro, additional, De Santis, Angelo, additional, Maugeri, Salvatore Roberto, additional, Perrone, Loredana, additional, Soldani, Maurizio, additional, Arena, Giovanni, additional, Brogi, Federico, additional, Calcara, Massimo, additional, Campuzano, Saioa A., additional, Cianchini, Gianfranco, additional, Del Corpo, Alfredo, additional, Di Mauro, Domenico, additional, Fidani, Cristiano, additional, Ippolito, Alessandro, additional, Lepidi, Stefania, additional, Marchetti, Dedalo, additional, Nardi, Adriano, additional, Orlando, Martina, additional, Piscini, Alessandro, additional, Regi, Mauro, additional, Sabbagh, Dario, additional, Zhima, Zeren, additional, and Yan, Rui, additional

Published
2022
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36. SLAMF1 regulation of chemotaxis and autophagy determines CLL patient response

Author

Bologna, Cinzia, Buonincontri, Roberta, Serra, Sara, Vaisitti, Tiziana, Audrito, Valentina, Brusa, Davide, Pagnani, Andrea, Coscia, Marta, D'Arena, Giovanni, Mereu, Elisabetta, Piva, Roberto, Furman, Richard R., Rossi, Davide, Gaidano, Gianluca, Terhorst, Cox, and Deaglio, Silvia

Subjects
Lymphocytic leukemia -- Research -- Development and progression -- Care and treatment -- Patient outcomes, Health care industry
Abstract

Chronic lymphocytic leukemia (CLL) is a variable disease; therefore, markers to identify aggressive forms are essential for patient management. Here, we have shown that expression of the costimulatory molecule and microbial sensor SLAMF1 (also known as CD150) is lost in a subset of patients with an aggressive CLL that associates with a shorter time to first treatment and reduced overall survival. SLAMF1 silencing in CLL-like Mec-1 cells, which constitutively express SLAMF1, modulated pathways related to cell migration, cytoskeletal organization, and intracellular vesicle formation and recirculation. SLAMF1 deficiency associated with increased expression of CXCR4, CD38, and CD44, thereby positively affecting chemotactic responses to CXCL12. SLAMF1 ligation with an agonistic monoclonal antibody increased ROS accumulation and induced phosphorylation of p38, JNK1/2, and BCL2, thereby promoting the autophagic flux. Beclin1 dissociated from BCL2 in response to SLAMF1 ligation, resulting in formation of the autophagy macrocomplex, which contains SLAMF1, beclin1, and the enzyme VPS34. Accordingly, SLAMF1-silenced cells or [SLAMF1.sup.lo] primary CLL cells were resistant to autophagy-activating therapeutic agents, such as fludarabine and the BCL2 homology domain 3 mimetic ABT-737. Together, these results indicate that loss of SLAMF1 expression in CLL modulates genetic pathways that regulate chemotaxis and autophagy and that potentially affect drug responses, and suggest that these effects underlie unfavorable clinical outcome experienced by [SLAMF1.sup.lo] patients., Introduction Chronic lymphocytic leukemia (CLL), the most common form of adult leukemia in Europe and North America, is characterized by the expansion of a population of mature B-lymphocytes that accumulate [...]

Published
2016

37. High serum levels of extracellular vesicles expressing malignancy-related markers are released in patients with various types of hematological neoplastic disorders

Author

Caivano, Antonella, Laurenzana, Ilaria, De Luca, Luciana, La Rocca, Francesco, Simeon, Vittorio, Trino, Stefania, D’Auria, Fiorella, Traficante, Antonio, Maietti, Maddalena, Izzo, Tiziana, D’Arena, Giovanni, Mansueto, Giovanna, Pietrantuono, Giuseppe, Laurenti, Luca, Musto, Pellegrino, and Del Vecchio, Luigi

Published
2015
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38. Flow cytometric evaluation of measurable residual disease in chronic lymphocytic leukemia: Where do we stand?

Author

D’Arena, Giovanni, primary, Sgambato, Alessandro, additional, Volpe, Silvestro, additional, Coppola, Giuseppe, additional, Amodeo, Rachele, additional, Tirino, Virginia, additional, D’Auria, Fiorella, additional, Statuto, Teodora, additional, Valvano, Luciana, additional, Pietrantuono, Giuseppe, additional, Deaglio, Silvia, additional, Efremov, Dimitar, additional, Laurenti, Luca, additional, and Aiello, Antonella, additional

Published
2022
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39. Design and Development of the New Diagnostics Control System for the SPES Project at INFN-LNL

Author

Savarese, Giovanni, Arena, Giovanni, Bortolato, Damiano, Gelain, Fabio, Marcato, Davide, Martinelli, Valentina, Munaron, Enrico, and Roetta, Marco

Subjects
Device Control and Integrating Diverse Systems, Accelerator Physics
Abstract

The need to get finer data to describe the beam is a relevant topic for all laboratories. For the SPES project at Laboratori Nazionali di Legnaro (LNL) a new diagnostic control system with more performing hardware, with respect to the one used in legacy accelerators based on Versabus Module Eurocard (VME) ADCs, has been developed. The new system uses a custom hardware to acquire signals in real time. These data and ancillary operations are managed by a control system based on the Experimental Physics and Industrial Control System (EPICS) standard and shown to users on a Control System Studio (CSS) graphical user interface. The new system improves the basic functionalities, current read-back over Beam Profilers (BP) and Faraday Cups (FC) and handlings control, with new features such as: multiple hardware gain levels selection, broken wires correction through polynomial interpolation and roto-translations taking into account alignment parameters. Another important feature, integrated with the usage of a python Finite State Machine (FSM), is the capability to control an emittance meter to quickly acquire data and calculate beam longitudinal phase space through the scubeex method., Proceedings of the 18th International Conference on Accelerator and Large Experimental Physics Control Systems, ICALEPCS2021, Shanghai, China

Published
2022
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40. Pysmlib: A Python Finite State Machine Library for EPICS

Author

Marcato, Davide, Arena, Giovanni, Bellato, Marco, Bortolato, Damiano, Gelain, Fabio, Lilli, Giordano, Martinelli, Valentina, Munaron, Enrico, Roetta, Marco, and Savarese, Giovanni

Subjects
Software Technology Evolution, Accelerator Physics
Abstract

In the field of Experimental Physics and Industrial Control Systems (EPICS)*, the traditional tool to implement high level procedures is the Sequencer*. While this is a mature, fast, and well-proven software, it comes with some drawbacks. For example, it���s based on a custom C-like programming language which may be unfamiliar to new users and it often results in complex, hard to read code. This paper presents pysmlib, a free and open source Python library developed as a simpler alternative to the EPICS Sequencer. The library exposes a simple interface to develop event-driven Finite State Machines (FSM), where the inputs are connected to Channel Access Process Variables (PV) thanks to the PyEpics** integration. Other features include parallel FSM with multi-threading support and input sharing, timers, and an integrated watchdog logic. The library offers a lower barrier to enter and greater extensibility thanks to the large ecosystem of scientific and engineering python libraries, making it a perfect fit for modern control system requirements. Pysmlib has been deployed in multiple projects at INFN Legnaro National Laboratories (LNL), proving its robustness and flexibility., Proceedings of the 18th International Conference on Accelerator and Large Experimental Physics Control Systems, ICALEPCS2021, Shanghai, China

Published
2022
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42. Subcutaneous immunoglobulins in chronic lymphocytic leukemia with secondary antibody deficiency. A monocentric experience during Covid‐19 pandemics

Author

Innocenti, Idanna, primary, Tomasso, Annamaria, additional, Benintende, Giulia, additional, Autore, Francesco, additional, Fresa, Alberto, additional, Vuono, Florenzia, additional, Stirparo, Luca, additional, Galli, Eugenio, additional, D’Arena, Giovanni, additional, Sorà, Federica, additional, Efremov, Dimitar, additional, and Laurenti, Luca, additional

Published
2022
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44. Atypical Mature T-Cell Neoplasms: The Relevance of the Role of Flow Cytometry

Author

Statuto, Teodora, D’Auria, Fiorella, Del Vecchio, Luigi, Mansueto, Giovanna Rosaria, Villani, Oreste, Lalinga, Anna Vittoria, Possidente, Luciana, Nozza, Filomena, Vona, Gabriella, Rago, Luciana, Storto, Giovanni, Gasparini, Vanessa Rebecca, Zambello, Renato, D’Arena, Giovanni, and Valvano, Luciana

Subjects
angioimmunoblastic t-cell lymphoma, diagnosis, peripheral t-cell lymphoma not otherwise specified, Angioimmunoblastic T-cell lymphoma, Diagnosis, Flow cytometry, Immunophenotype, Peripheral T-cell lymphoma not otherwise specified, T-cell prolymphocytic leukemia, flow cytometry, Case Series, t-cell prolymphocytic leukemia, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, lcsh:RC254-282, OncoTargets and Therapy, immunophenotype
Abstract

Teodora Statuto,1,* Fiorella D’Auria,2,* Luigi Del Vecchio3,4,†, Giovanna Rosaria Mansueto,5 Oreste Villani,5 Anna Vittoria Lalinga6,†, Luciana Possidente,6 Filomena Nozza,1 Gabriella Vona,1 Luciana Rago,7 Giovanni Storto,8 Vanessa Rebecca Gasparini,9 Renato Zambello,10 Giovanni D’Arena,5,* Luciana Valvano1,* 1Laboratory of Clinical Research and Advanced Diagnostics, Centro Di Riferimento Oncologico Della Basilicata (IRCCS-CROB), Rionero in Vulture, Pz, Italy; 2Unit of Clinical Pathology, Centro Di Riferimento Oncologico Della Basilicata (IRCCS-CROB), Rionero in Vulture, Pz, Italy; 3CEINGE Biotecnologie Avanzate S.c.a.r.l, Federico II University, Naples, Italy; 4Department of Molecular Medicine and Medical Biotechnology (DMMBM), Federico II University, Naples, Italy; 5Hematology Department of Basilicata, Centro Di Riferimento Oncologico Della Basilicata (IRCCS-CROB), Rionero in Vulture, Pz, Italy; 6Pathology Unit, Centro Di Riferimento Oncologico Della Basilicata (IRCCS-CROB), Rionero in Vulture, Pz, Italy; 7Radiotherapy Unit, Centro Di Riferimento Oncologico Della Basilicata (IRCCS-CROB), Rionero in Vulture, Pz, Italy; 8Department of Nuclear Medicine, Centro Di Riferimento Oncologico Della Basilicata (IRCCS-CROB), Rionero in Vulture, Pz, Italy; 9Department of Medicine, University of Padova - Veneto Institute of Molecular Medicine, VIMM, Padova, PD, Italy; 10Hematology and Clinical Immunology, Department of Medicine, Padua School of Medicine, Padova, PD, Italy†Anna Vittoria Lalinga passed away on January 26, 2020 and Luigi Del Vecchio passed away on August 16, 2018*These authors contributed equally to this workCorrespondence: Luciana ValvanoLaboratory of Clinical Research and Advanced Diagnostics, Centro Di Riferimento Oncologico Della Basilicata (IRCCS-CROB), Rionero in Vulture, Pz, ItalyTel +39 0972 726395Fax +39 0972 723509Email luciana.valvano@crob.itAbstract: Lymphoproliferative disorders are a heterogeneous group of malignant clonal proliferations of lymphocytes whose diagnosis remains challenging, despite diagnostic criteria are now well established, due to their heterogeneity in clinical presentation and immunophenotypic profile. Lymphoid T-cell disorders are more rarely seen than B-cell entities and more difficult to diagnose for the absence of a specific immunophenotypic signature. Flow cytometry is a useful tool in diagnosing T-cell lymphoproliferative disorders since it is not only able to better characterize T-cell neoplasms but also to resolve some very complicated cases, in particular those in which a small size population of neoplastic cells is available for the analysis. Here, we report three patients with mature T-cell neoplasms with atypical clinical and biological features in which analysis of peripheral blood and bone marrow specimens by means of multicolor flow cytometry was very useful to identify and characterize three rare T-cell lymphoproliferative disorders, such as angioimmunoblastic T-cell lymphoma, peripheral T-cell lymphoma not otherwise specified and T-cell prolymphocytic leukemia. The aim of this case series report is not only to describe three rare cases of lymphoproliferative neoplasms but also to raise awareness that a fast, highly sensitive, and reproducible procedure, such as flow cytometry immunophenotyping, can have a determinant diagnostic role in these patients.Keywords: flow cytometry, immunophenotype, angioimmunoblastic T-cell lymphoma, peripheral T-cell lymphoma not otherwise specified, T-cell prolymphocytic leukemia, diagnosis

Published
2020

47. Italian Cytometry Society (GIC) endorsement of consensus recommendations for measurable residual disease in chronic lymphocytic leukemia

Author

D'Arena, Giovanni, primary, Volpe, Silvestro, additional, Amodeo, Rachele, additional, Tirino, Virginia, additional, D'Auria, Fiorella, additional, Annamaria, Giordano, additional, Pietrantuono, Giuseppe, additional, Laurenti, Luca, additional, Aiello, Antonella, additional, and Musto, Pellegrino, additional

Published
2021
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49. Flow cytometric evaluation of measurable residual disease in chronic lymphocytic leukemia: Where do we stand?

Author

D'Arena, Giovanni, Sgambato, Alessandro, Volpe, Silvestro, Coppola, Giuseppe, Amodeo, Rachele, Tirino, Virginia, D'Auria, Fiorella, Statuto, Teodora, Valvano, Luciana, Pietrantuono, Giuseppe, Deaglio, Silvia, Efremov, Dimitar, Laurenti, Luca, and Aiello, Antonella

Subjects
CHRONIC lymphocytic leukemia, CHRONIC diseases, CHRONIC leukemia, FLOW cytometry, TREATMENT effectiveness
Abstract

Measurable residual disease (MRD) has emerged as a relevant parameter of response to therapy in chronic lymphocytic leukemia (CLL). Although several methods have been developed, flow cytometry has emerged as the most useful and standardized approach to measure and quantify MRD. The improved sensitivity of MRD measurements has been paralleled by the development of more effective therapeutic strategies for CLL, increasing the applicability of MRD detection in this setting. Chemotherapy and chemoimmunotherapy have firstly demonstrated their ability to obtain a deep MRD. Combined targeted therapies are also demonstrating a high molecular response rate and prospective trials are exploring the role of MRD to guide the duration of treatment in this setting. In this review we briefly summarize what we have learned about MRD with emphasis on its flow cytometric detection. [ABSTRACT FROM AUTHOR]

Published
2022
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50. CD200 Baseline Serum Levels Predict Prognosis of Chronic Lymphocytic Leukemia

Author

D’Arena, Giovanni, primary, Vitale, Candida, additional, Coscia, Marta, additional, Lamorte, Daniela, additional, Pietrantuono, Giuseppe, additional, Perutelli, Francesca, additional, D’Auria, Fiorella, additional, Statuto, Teodora, additional, Valvano, Luciana, additional, Tomasso, Annamaria, additional, Griggio, Valentina, additional, Jones, Rebecca, additional, Mansueto, Giovanna, additional, Villani, Oreste, additional, D’Agostino, Simona, additional, Viglioglia, Vito, additional, De Feo, Vincenzo, additional, Calapai, Fabrizio, additional, Mannucci, Carmen, additional, Sgambato, Alessandro, additional, Efremov, Dimitar G., additional, and Laurenti, Luca, additional

Published
2021
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