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1. Benchmarking the design of the cryogenics system for the underground argon in DarkSide-20k

Author

Collaboration, DarkSide-20k, Acerbi, F., Adhikari, P., Agnes, P., Ahmad, I., Albergo, S., Albuquerque, I. F. M., Alexander, T., Alton, A. K., Amaudruz, P., Angiolilli, M., Aprile, E., Ardito, R., Corona, M. Atzori, Auty, D. J., Ave, M., Avetisov, I. C., Azzolini, O., Back, H. O., Balmforth, Z., Olmedo, A. Barrado, Barrillon, P., Batignani, G., Bhowmick, P., Blua, S., Bocci, V., Bonivento, W., Bottino, B., Boulay, M. G., Buchowicz, A., Bussino, S., Busto, J., Cadeddu, M., Cadoni, M., Calabrese, R., Camillo, V., Caminata, A., Canci, N., Capra, A., Caravati, M., Cárdenas-Montes, M., Cargioli, N., Carlini, M., Castellani, A., Castello, P., Cavalcante, P., Cebrian, S., Ruiz, J. Cela, Chashin, S., Chepurnov, A., Cifarelli, L., Cintas, D., Citterio, M., Cleveland, B., Coadou, Y., Cocco, V., Colaiuda, D., Vilda, E. Conde, Consiglio, L., Costa, B. S., Czubak, M., D'Aniello, M., D'Auria, S., Rolo, M. D. Da Rocha, Darbo, G., Davini, S., De Cecco, S., De Guido, G., Dellacasa, G., Derbin, A. V., Devoto, A., Di Capua, F., Di Ludovico, A., Di Noto, L., Di Stefano, P., Dias, L. K., Mairena, D. Díaz, Ding, X., Dionisi, C., Dolganov, G., Dordei, F., Dronik, V., Elersich, A., Ellingwood, E., Erjavec, T., Diaz, M. Fernandez, Ficorella, A., Fiorillo, G., Franchini, P., Franco, D., Gatti, H. Frandini, Frolov, E., Gabriele, F., Gahan, D., Galbiati, C., Galiński, G., Gallina, G., Gallus, G., Garbini, M., Abia, P. Garcia, Gawdzik, A., Gendotti, A., Ghisi, A., Giovanetti, G. K., Casanueva, V. Goicoechea, Gola, A., Grandi, L., Grauso, G., di Cortona, G. Grilli, Grobov, A., Gromov, M., Guerzoni, M., Gulino, M., Guo, C., Hackett, B. R., Hallin, A., Hamer, A., Haranczyk, M., Harrop, B., Hessel, T., Hill, S., Horikawa, S., Hu, J., Hubaut, F., Hucker, J., Hugues, T., Hungerford, E. V., Ianni, A., Ippolito, V., Jamil, A., Jillings, C., Jois, S., Kachru, P., Keloth, R., Kemmerich, N., Kemp, A., Kendziora, C. L., Kimura, M., Kish, A., Kondo, K., Korga, G., Kotsiopoulou, L., Koulosousas, S., Kubankin, A., Kunzé, P., Kuss, M., Kuźniak, M., Kuzwa, M., La Commara, M., Lai, M., Guirriec, E. Le, Leason, E., Leoni, A., Lidey, L., Lissia, M., Luzzi, L., Lychagina, O., Macfadyen, O., Machulin, I. N., Manecki, S., Manthos, I., Mapelli, L., Marasciulli, A., Mari, S. M., Mariani, C., Maricic, J., Martinez, M., Martoff, C. J., Matteucci, G., Mavrokoridis, K., McDonald, A. B., Mclaughlin, J., Merzi, S., Messina, A., Milincic, R., Minutoli, S., Mitra, A., Moharana, A., Moioli, S., Monroe, J., Moretti, E., Morrocchi, M., Mroz, T., Muratova, V. N., Murphy, M., Murra, M., Muscas, C., Musico, P., Nania, R., Nessi, M., Nieradka, G., Nikolopoulos, K., Nikoloudaki, E., Nowak, J., Olchanski, K., Oleinik, A., Oleynikov, V., Organtini, P., de Solórzano, A. Ortiz, Pallavicini, M., Pandola, L., Pantic, E., Paoloni, E., Papi, D., Pastuszak, G., Paternoster, G., Peck, A., Pegoraro, P. A., Pelczar, K., Pellegrini, L. A., Perez, R., Perotti, F., Pesudo, V., Piacentini, S. I., Pino, N., Plante, G., Pocar, A., Poehlmann, M., Pordes, S., Pralavorio, P., Price, D., Puglia, S., Bazetto, M. Queiroga, Ragusa, F., Ramachers, Y., Ramirez, A., Ravinthiran, S., Razeti, M., Renshaw, A. L., Rescigno, M., Retiere, F., Rignanese, L. P., Rivetti, A., Roberts, A., Roberts, C., Rogers, G., Romero, L., Rossi, M., Rubbia, A., Rudik, D., Sabia, M., Salomone, P., Samoylov, O., Sandford, E., Sanfilippo, S., Santone, D., Santorelli, R., Santos, E. M., Savarese, C., Scapparone, E., Schillaci, G., Schuckman II, F. G., Scioli, G., Semenov, D. A., Shalamova, V., Sheshukov, A., Simeone, M., Skensved, P., Skorokhvatov, M. D., Smirnov, O., Smirnova, T., Smith, B., Sotnikov, A., Spadoni, F., Spangenberg, M., Stefanizzi, R., Steri, A., Stornelli, V., Stracka, S., Sulis, S., Sung, A., Sunny, C., Suvorov, Y., Szelc, A. M., Taborda, O., Tartaglia, R., Taylor, A., Taylor, J., Tedesco, S., Testera, G., Thieme, K., Thompson, A., Thorpe, T. N., Tonazzo, A., Torres-Lara, S., Tricomi, A., Unzhakov, E. V., Vallivilayil, T. J., Van Uffelen, M., Velazquez-Fernandez, L., Viant, T., Viel, S., Vishneva, A., Vogelaar, R. B., Vossebeld, J., Vyas, B., Wada, M., Walczak, M. B., Wang, H., Wang, Y., Westerdale, S., Williams, L., Wojaczyński, R., Wojcik, M., Wojcik, M. M., Wright, T., Xiao, X., Xie, Y., Yang, C., Yin, J., Zabihi, A., Zakhary, P., Zani, A., Zhang, Y., Zhu, T., Zichichi, A., Zuzel, G., and Zykova, M. P.

Subjects
Physics - Instrumentation and Detectors, High Energy Physics - Experiment
Abstract

DarkSide-20k (DS-20k) is a dark matter detection experiment under construction at the Laboratori Nazionali del Gran Sasso (LNGS) in Italy. It utilises ~100 t of low radioactivity argon from an underground source (UAr) in its inner detector, with half serving as target in a dual-phase time projection chamber (TPC). The UAr cryogenics system must maintain stable thermodynamic conditions throughout the experiment's lifetime of >10 years. Continuous removal of impurities and radon from the UAr is essential for maximising signal yield and mitigating background. We are developing an efficient and powerful cryogenics system with a gas purification loop with a target circulation rate of 1000 slpm. Central to its design is a condenser operated with liquid nitrogen which is paired with a gas heat exchanger cascade, delivering a combined cooling power of >8 kW. Here we present the design choices in view of the DS-20k requirements, in particular the condenser's working principle and the cooling control, and we show test results obtained with a dedicated benchmarking platform at CERN and LNGS. We find that the thermal efficiency of the recirculation loop, defined in terms of nitrogen consumption per argon flow rate, is 95 % and the pressure in the test cryostat can be maintained within $\pm$(0.1-0.2) mbar. We further detail a 5-day cool-down procedure of the test cryostat, maintaining a cooling rate typically within -2 K/h, as required for the DS-20k inner detector. Additionally, we assess the circuit's flow resistance, and the heat transfer capabilities of two heat exchanger geometries for argon phase change, used to provide gas for recirculation. We conclude by discussing how our findings influence the finalisation of the system design, including necessary modifications to meet requirements and ongoing testing activities., Comment: 45 pages, 24 figures

Published
2024

2. DarkSide-20k sensitivity to light dark matter particles

Author

Collaboration, DarkSide-20k, Acerbi, F., Adhikari, P., Agnes, P., Ahmad, I., Albergo, S., Albuquerque, I. F. M., Alexander, T., Alton, A. K., Amaudruz, P., Angiolilli, M., Aprile, E., Ardito, R., Corona, M. Atzori, Auty, D. J., Ave, M., Avetisov, I. C., Azzolini, O., Back, H. O., Balmforth, Z., Olmedo, A. Barrado, Barrillon, P., Batignani, G., Bhowmick, P., Blua, S., Bocci, V., Bonivento, W., Bottino, B., Boulay, M. G., Buchowicz, A., Bussino, S., Busto, J., Cadeddu, M., Cadoni, M., Calabrese, R., Camillo, V., Caminata, A., Canci, N., Capra, A., Caravati, M., Cárdenas-Montes, M., Cargioli, N., Carlini, M., Castellani, A., Castello, P., Cavalcante, P., Cebrian, S., Ruiz, J. M. Cela, Chashin, S., Chepurnov, A., Cifarelli, L., Cintas, D., Citterio, M., Cleveland, B., Coadou, Y., Cocco, V., Colaiuda, D., Vilda, E. Conde, Consiglio, L., Costa, B. S., Czubak, M., D'Aniello, M., D'Auria, S., Rolo, M. D. Da Rocha, Darbo, G., Davini, S., De Cecco, S., De Guido, G., Dellacasa, G., Derbin, A. V., Devoto, A., Di Capua, F., Di Ludovico, A., Di Noto, L., Di Stefano, P., Dias, L. K., Mairena, D. Díaz, Ding, X., Dionisi, C., Dolganov, G., Dordei, F., Dronik, V., Elersich, A., Ellingwood, E., Erjavec, T., Diaz, M. Fernandez, Ficorella, A., Fiorillo, G., Franchini, P., Franco, D., Gatti, H. Frandini, Frolov, E., Gabriele, F., Gahan, D., Galbiati, C., Galiński, G., Gallina, G., Gallus, G., Garbini, M., Abia, P. Garcia, Gawdzik, A., Gendotti, A., Ghisi, A., Giovanetti, G. K., Casanueva, V. Goicoechea, Gola, A., Grandi, L., Grauso, G., di Cortona, G. Grilli, Grobov, A., Gromov, M., Guerzoni, M., Gulino, M., Guo, C., Hackett, B. R., Hallin, A., Hamer, A., Haranczyk, M., Harrop, B., Hessel, T., Hill, S., Horikawa, S., Hu, J., Hubaut, F., Hucker, J., Hugues, T., Hungerford, E. V., Ianni, A., Ippolito, V., Jamil, A., Jillings, C., Jois, S., Kachru, P., Keloth, R., Kemmerich, N., Kemp, A., Kendziora, C. L., Kimura, M., Kondo, K., Korga, G., Kotsiopoulou, L., Koulosousas, S., Kubankin, A., Kunzé, P., Kuss, M., Kuźniak, M., Kuzwa, M., La Commara, M., Lai, M., Guirriec, E. Le, Leason, E., Leoni, A., Lidey, L., Lissia, M., Luzzi, L., Lychagina, O., Macfadyen, O., Machulin, I. N., Manecki, S., Manthos, I., Mapelli, L., Marasciulli, A., Mari, S. M., Mariani, C., Maricic, J., Martinez, M., Martoff, C. J., Matteucci, G., Mavrokoridis, K., McDonald, A. B., Mclaughlin, J., Merzi, S., Messina, A., Milincic, R., Minutoli, S., Mitra, A., Moioli, S., Monroe, J., Moretti, E., Morrocchi, M., Mroz, T., Muratova, V. N., Murphy, M., Murra, M., Muscas, C., Musico, P., Nania, R., Nessi, M., Nieradka, G., Nikolopoulos, K., Nikoloudaki, E., Nowak, J., Olchanski, K., Oleinik, A., Oleynikov, V., Organtini, P., de Solórzano, A. Ortiz, Pallavicini, M., Pandola, L., Pantic, E., Paoloni, E., Papi, D., Pastuszak, G., Paternoster, G., Peck, A., Pegoraro, P. A., Pelczar, K., Pellegrini, L. A., Perez, R., Perotti, F., Pesudo, V., Piacentini, S., Pino, N., Plante, G., Pocar, A., Poehlmann, M., Pordes, S., Pralavorio, P., Price, D., Puglia, S., Bazetto, M. Queiroga, Ragusa, F., Ramachers, Y., Ramirez, A., Ravinthiran, S., Razeti, M., Renshaw, A. L., Rescigno, M., Retiere, F., Rignanese, L. P., Rivetti, A., Roberts, A., Roberts, C., Rogers, G., Romero, L., Rossi, M., Rubbia, A., Rudik, D., Sabia, M., Salomone, P., Samoylov, O., Sandford, E., Sanfilippo, S., Santone, D., Santorelli, R., Santos, E. M., Savarese, C., Scapparone, E., Schillaci, G., Schuckman II, F. G., Scioli, G., Semenov, D. A., Shalamova, V., Sheshukov, A., Simeone, M., Skensved, P., Skorokhvatov, M. D., Smirnov, O., Smirnova, T., Smith, B., Sotnikov, A., Spadoni, F., Spangenberg, M., Stefanizzi, R., Steri, A., Stornelli, V., Stracka, S., Sulis, S., Sung, A., Sunny, C., Suvorov, Y., Szelc, A. M., Taborda, O., Tartaglia, R., Taylor, A., Taylor, J., Tedesco, S., Testera, G., Thieme, K., Thompson, A., Tonazzo, A., Torres-Lara, S., Tricomi, A., Unzhakov, E. V., Vallivilayil, T. J., Van Uffelen, M., Velazquez-Fernandez, L., Viant, T., Viel, S., Vishneva, A., Vogelaar, R. B., Vossebeld, J., Vyas, B., Walczak, M. B., Wang, Y., Wang, H., Westerdale, S., Williams, L., Wojaczyński, R., Wojcik, M., Wojcik, M. M., Wright, T., Xie, Y., Yang, C., Yin, J., Zabihi, A., Zakhary, P., Zani, A., Zhang, Y., Zhu, T., Zichichi, A., Zuzel, G., and Zykova, M. P.

Subjects
High Energy Physics - Experiment, Astrophysics - Cosmology and Nongalactic Astrophysics
Abstract

The dual-phase liquid argon time projection chamber is presently one of the leading technologies to search for dark matter particles with masses below 10 GeV/c$^2$. This was demonstrated by the DarkSide-50 experiment with approximately 50 kg of low-radioactivity liquid argon as target material. The next generation experiment DarkSide-20k, currently under construction, will use 1,000 times more argon and is expected to start operation in 2027. Based on the DarkSide-50 experience, here we assess the DarkSide-20k sensitivity to models predicting light dark matter particles, including Weakly Interacting Massive Particles (WIMPs) and sub-GeV/c$^2$ particles interacting with electrons in argon atoms. With one year of data, a sensitivity improvement to dark matter interaction cross-sections by at least one order of magnitude with respect to DarkSide-50 is expected for all these models. A sensitivity to WIMP--nucleon interaction cross-sections below $1\times10^{-42}$ cm$^2$ is achievable for WIMP masses above 800 MeV/c$^2$. With 10 years exposure, the neutrino fog can be reached for WIMP masses around 5 GeV/c$^2$., Comment: 13 pages, 4 figures, supplementary material (4 figures)

Published
2024
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3. Directionality of nuclear recoils in a liquid argon time projection chamber

Author

Collaboration, The DarkSide-20k, Agnes, P., Ahmad, I., Albergo, S., Albuquerque, I. F. M., Alexander, T., Alton, A. K., Amaudruz, P., Corona, M. Atzori, Ave, M., Avetisov, I. Ch., Azzolini, O., Back, H. O., Balmforth, Z., Barrado-Olmedo, A., Barrillon, P., Basco, A., Batignani, G., Bocci, V., Bonivento, W. M., Bottino, B., Boulay, M. G., Busto, J., Cadeddu, M., Caminata, A., Canci, N., Cappello, G., Capra, A., Caprioli, S., Caravati, M., Cargioli, N., Carlini, M., Castello, P., Cataudella, V., Cavalcante, P., Cavuoti, S., Cebrian, S., Ruiz, J. M. Cela, Chashin, S., Chepurnov, A., Chyhyrynets, E., Cifarelli, L., Cintas, D., Citterio, M., Cleveland, B., Cocco, V., Vilda, E. Conde, Consiglio, L., Copello, S., Covone, G., Czubak, M., D'Aniello, M., D'Auria, S., Rolo, M. D. Da Rocha, Davini, S., de Candia, A., De Cecco, S., De Gruttola, D., De Filippis, G., Dell'Aquila, D., De Pasquale, S., De Rosa, G., Dellacasa, G., Derbin, A. V., Devoto, A., Di Capua, F., Di Noto, L., Dionisi, C., Di Stefano, P., Dolganov, G., Dordei, F., Elersich, A., Ellingwood, E., Erjavec, T., Diaz, M. Fernandez, Fiorillo, G., Franchini, P., Franco, D., Funicello, N., Gabriele, F., Gahan, D., Galbiati, C., Gallina, G., Gallus, G., Garbini, M., Abia, P. Garcia, Gendotti, A., Ghiano, C., Giganti, C., Giovanetti, G. K., Casanueva, V. Goicoechea, Gola, A., Grauso, G., di Cortona, G. Grilli, Grobov, A., Gromov, M., Guan, M., Guerzoni, M., Gulino, M., Guo, C., Hackett, B. R., Hallin, A. L., Hamer, A., Haranczyk, M., Hessel, T., Hill, S., Horikawa, S., Hubaut, F., Hucker, J., Hugues, T., Ianni, An., Ippolito, V., Jillings, C., Jois, S., Kachru, P., Kemmerich, N., Kemp, A. A., Kendziora, C. L., Kimura, M., Kochanek, I., Kondo, K., Korga, G., Koulosousas, S., Kubankin, A., Kuss, M., Kuzniak, M., La Commara, M., Lai, M., Guirriec, E. Le, Leason, E., Leoni, A., Li, X., Lidey, L., Lissia, M., Luzzi, L., Lychagina, O., Macfadyen, O., Machulin, I. N., Manecki, S., Manthos, I., Mapelli, L., Margotti, A., Marik, S. M., Mariani, C., Maricic, J., Marini, A., Martínez, M., Martoff, C. J., Matteucci, G., Mavrokoridis, K., McDonald, A. B., Messina, A., Milincic, R., Mitra, A., Moharana, A., Monroe, J., Moretti, E., Morrocchi, M., Mróz, T., Muratova, V. N., Muscas, C., Musico, P., Nania, R., Nessi, M., Nieradka, G., Nikolopoulos, K., Nowak, J., Olchansky, K., Oleinik, A., Oleynikov, V., Organtini, P., de Solórzano, A. Ortiz, Pagani, L., Pallavicini, M., Pandola, L., Pantic, E., Paoloni, E., Paternoster, G., Pegoraro, P. A., Pelczar, K., Pesudo, V., Piacentini, S., Pino, N., Pocar, A., Poehlmann, D. M., Pordes, S., Pralavorio, P., Price, D., Ragusa, F., Ramachers, Y., Razeti, M., Renshaw, A. L., Rescigno, M., Retiere, F., Rignanese, L. P., Ripoli, C., Rivetti, A., Roberts, A., Roberts, C., Rode, J., Rogers, G., Romero, L., Rossi, M., Rubbia, A., Sabia, M. A., Salomone, P., Sandford, E., Sanfilippo, S., Santone, D., Santorelli, R., Savarese, C., Scapparone, E., Schillaci, G., Schuckman II, F. G., Scioli, G., Simeone, M., Skensved, P., Skorokhvatov, M. D., Smirnov, O., Smirnova, T., Smith, B., Sosa, A., Spadoni, F., Spangenberg, M., Stefanizzi, R., Steri, A., Stornelli, V., Stracka, S., Stringer, M., Sulis, S., Sung, A., Suvorov, Y., Szelc, A. M., Tartaglia, R., Taylor, A., Taylor, J., Tedesco, S., Testera, G., Thieme, K., Thorpe, T. N., Tonazzo, A., Tricomi, A., Unzhakov, E. V., John, T. Vallivilayil, Van Uffelen, M., Viant, T., Viel, S., Vogelaar, R. B., Vossebeld, J., Wada, M., Walczak, M. B., Wang, H., Wang, Y., Westerdale, S., Williams, L., Wingerter-Seez, I., Wojaczynski, R., Wojcik, Ma. M., Wright, T., Xie, Y., Yang, C., Zabihi, A., Zakhary, P., Zani, A., Zichichi, A., Zuzel, G., and Zykova, M. P.

Subjects
Physics - Instrumentation and Detectors
Abstract

The direct search for dark matter in the form of weakly interacting massive particles (WIMP) is performed by detecting nuclear recoils (NR) produced in a target material from the WIMP elastic scattering. A promising experimental strategy for direct dark matter search employs argon dual-phase time projection chambers (TPC). One of the advantages of the TPC is the capability to detect both the scintillation and charge signals produced by NRs. Furthermore, the existence of a drift electric field in the TPC breaks the rotational symmetry: the angle between the drift field and the momentum of the recoiling nucleus can potentially affect the charge recombination probability in liquid argon and then the relative balance between the two signal channels. This fact could make the detector sensitive to the directionality of the WIMP-induced signal, enabling unmistakable annual and daily modulation signatures for future searches aiming for discovery. The Recoil Directionality (ReD) experiment was designed to probe for such directional sensitivity. The TPC of ReD was irradiated with neutrons at the INFN Laboratori Nazionali del Sud, and data were taken with 72 keV NRs of known recoil directions. The direction-dependent liquid argon charge recombination model by Cataudella et al. was adopted and a likelihood statistical analysis was performed, which gave no indications of significant dependence of the detector response to the recoil direction. The aspect ratio R of the initial ionization cloud is estimated to be 1.037 +/- 0.027 and the upper limit is R < 1.072 with 90% confidence level, Comment: 20 pages, 10 figures, submitted to Eur. Phys. J. C

Published
2023

4. Sensitivity projections for a dual-phase argon TPC optimized for light dark matter searches through the ionization channel

Author

Agnes, P., Ahmad, I., Albergo, S., Albuquerque, I. F. M., Alexander, T., Alton, A. K., Amaudruz, P., Corona, M. Atzori, Auty, D. J., Ave, M., Avetisov, I. Ch., Avetisov, R. I., Azzolini, O., Back, H. O., Balmforth, Z., Barbarian, V., Olmedo, A. Barrado, Barrillon, P., Basco, A., Batignani, G., Berzin, E., Bondar, A., Bonivento, W. M., Borisova, E., Bottino, B., Boulay, M. G., Buccino, G., Bussino, S., Busto, J., Buzulutskov, A., Cadeddu, M., Cadoni, M., Caminata, A., Canci, N., Capra, A., Caprioli, S., Caravati, M., Cárdenas-Montes, M., Cargioli, N., Carlini, M., Castello, P., Cataudella, V., Cavalcante, P., Cavuoti, S., Cebrian, S., Ruiz, J. M. Cela, Chashin, S., Chepurnov, A., Chyhyrynets, E, Cicalò, C., Cifarelli, L., Cintas, D., Cocco, V., Vilda, E. Conde, Consiglio, L., Copello, S., Covone, G., Cross, S., Czudak, P., D'Aniello, M., D'Auria, S., Rolo, M. D. Da Rocha, Dadoun, O., Daniel, M., Davini, S., De Candia, A., De Cecco, S., De Falco, A., De Filippis, G., De Gruttola, D., De Pasquale, S., De Rosa, G., Dellacasa, G., Derbin, A. V., Devoto, A., Di Capua, F., Di Noto, L., Di Stefano, P., Dionisi, C., Dolganov, G., Dordei, F., Doria, L., Erjavec, T., Diaz, M. Fernandez, Fiorillo, G., Franceschi, A., Franchini, P., Franco, D., Frolov, E., Funicello, N., Gabriele, F., Gahan, D., Galbiati, C., Gallina, G., Gallus, G., Garbini, M., Abia, P. Garcia, Gendotti, A., Ghiano, C., Giampaolo, R. A., Giganti, C., Giorgi, M. A., Giovanetti, G. K., Casanueva, V. Goicoechea, Gola, A., Gorman, D., Diaz, R. Graciani, Grauso, G., di Cortona, G. Grilli, Grobov, A., Gromov, M., Guan, M., Guerzoni, M., Gulino, M., Guo, C., Hackett, B. R., Hall, J. B., Hallin, A. L., Hamer, A., Helton, H., Haranczyk, M., Hessel, T., Hill, S., Horikawa, S., Hubaut, F., Hugues, T., Hungerford, E. V., Ianni, An., Ippolito, V., Jillings, C., Kachru, P., Kemp, A. A., Kendziora, C. L., Keppel, G., Khomyakov, A. V., Kimura, M., Kochanek, I., Kondo, K., Korga, G., Koulosousas, S., Kubankin, A., Kuss, M., Kuźniak, M., La Commara, M., Lai, M., Guirriec, E. Le, Leason, E., Li, X., Lidey, L., Lipp, J., Lissia, M., Longo, G., Luzzi, L., Macfadyen, O., Machulin, I. N., Manthos, I., Mapelli, L., Margotti, A., Mari, S. M., Mariani, C., Maricic, J., Marini, A., Martínez, M., Martoff, C. J., Masoni, A., Mavrokoridis, K., Mazzi, A., McDonald, A. B., Messina, A., Milincic, R., Moggi, A., Moharana, A., Monroe, J., Morrocchi, M., Mozhevitina, E. N., Mróz, T., Muratova, V. N., Muscas, C., Musico, P., Nania, R., Napolitano, T., Nessi, M., Nieradka, G., Nikolopoulos, K., Nikulin, I., Nowak, J., Olchansky, K., Oleinik, A., Oleynikov, V., Organtini, P., de Solórzano, A. Ortiz, Pagani, L., Pallavicini, M., Pandola, L., Pantic, E., Paoloni, E., Paternoster, G., Pegoraro, P. A., Pelczar, K., Pellegrino, C., Perotti, F., Pesudo, V., Piacentini, S., Pietropaolo, F., Pino, N., Pira, C., Pocar, A., Poehlmann, D. M., Pordes, S., Pralavorio, P., Price, D., Raffaelli, F., Ragusa, F., Ramachers, Y., Ramirez, A., Razeti, M., Razeto, A., Renshaw, A. L., Rescigno, M., Resnati, F., Retiere, F., Rignanese, L. P., Ripoli, C., Rivetti, A., Roberts, A., Roberts, C., Rode, J., Rogers, G., Romero, L., Rossi, M., Rubbia, A., Sadashivajois, S., Saffold, T. R., Samoylov, O., Sandford, E., Sanfilippo, S., Santone, D., Santorelli, R., Savarese, C., Scapparone, E., Scioli, G., Semenov, D. A., Shchagin, A., Sheshukov, A., Simeone, M., Skensved, P., Skorokhvatov, M. D., Smirnov, O., Smirnova, T., Smith, B., Sokolov, A., Spangenberg, M., Stefanizzi, R., Steri, A., Stracka, S., Strickland, V., Stringer, M., Sulis, S., Sung, A., Suvorov, Y., Szelc, A. M., Türkoğ, C., Tartaglia, R., Taylor, A., Taylor, J., Tedesco, S., Testera, G., Thieme, K., Thorpe, T. N., Tonazzo, A., Torres-Lara, S., Tricomi, A., Unzhakov, E. V., John, T. Vallivilayil, Van Uffelen, M., Viant, T., Viel, S., Vishneva, A., Vogelaar, R. B., Vossebeld, J., Wada, M., Walczak, M. B., Wang, Y., Westerdale, S., Wheadon, R. J., Williams, L., Wingerter-Seez, I., Wojaczyński, R., Wojcik, Ma. M., Wojcik, Ma., Wright, T., Xie, Y., Yang, C., Zabihi, A., Zakhary, P., Zani, A., Zichichi, A., Zuzel, G., and Zykova, M. P.

Subjects
Physics - Instrumentation and Detectors, High Energy Physics - Experiment
Abstract

Dark matter lighter than 10 GeV/c$^2$ encompasses a promising range of candidates. A conceptual design for a new detector, DarkSide-LowMass, is presented, based on the DarkSide-50 detector and progress toward DarkSide-20k, optimized for a low-threshold electron-counting measurement. Sensitivity to light dark matter is explored for various potential energy thresholds and background rates. These studies show that DarkSide-LowMass can achieve sensitivity to light dark matter down to the solar neutrino floor for GeV-scale masses and significant sensitivity down to 10 MeV/c$^2$ considering the Migdal effect or interactions with electrons. Requirements for optimizing the detector's sensitivity are explored, as are potential sensitivity gains from modeling and mitigating spurious electron backgrounds that may dominate the signal at the lowest energies.

Published
2022
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5. Separating $^{39}$Ar from $^{40}$Ar by cryogenic distillation with Aria for dark matter searches

Author

DarkSide Collaboration, Agnes, P., Albergo, S., Albuquerque, I. F. M., Alexander, T., Alici, A., Alton, A. K., Amaudruz, P., Arba, M., Arpaia, P., Arcelli, S., Ave, M., Avetissov, I. Ch., Avetisov, R. I., Azzolini, O., Back, H. O., Balmforth, Z., Barbarian, V., Olmedo, A. Barrado, Barrillon, P., Basco, A., Batignani, G., Bondar, A., Bonivento, W. M., Borisova, E., Bottino, B., Boulay, M. G., Buccino, G., Bussino, S., Busto, J., Buzulutskov, A., Cadeddu, M., Cadoni, M., Caminata, A., Canesi, E. V., Canci, N., Cappello, G., Caravati, M., C, M., Cargioli, N., Carlini, M., Carnesecchi, F., Castello, P., Castellani, A., Catalanotti, S., Cataudella, V., Cavalcante, P., Cavuoti, S., Cebrian, S., Ruiz, J. M. Cela, Celano, B., Chashin, S., Chepurnov, A., Cical, C., Cifarelli, L., Cintas, D., Coccetti, F., Cocco, V., Colocci, M., Vilda, E. Conde, Consiglio, L., Copello, S., Corning, J., Covone, G., Czudak, P., D'Aniello, M., D'Auria, S., Rolo, M. D. Da Rocha, Dadoun, O., Daniel, M., Davini, S., De Candia, A., De Cecco, S., De Falco, A., De Filippis, G., De Gruttola, D., De Guido, G., De Rosa, G., Della Valle, M., Dellacasa, G., De Pasquale, S., Derbin, A. V., Devoto, A., Di Noto, L., Di Eusanio, F., Dionisi, C., Di Stefano, P., Dolganov, G., Dongiovanni, D., Dordei, F., Downing, M., Erjavec, T., Falciano, S., Farenzena, S., Diaz, M. Fernandez, Filip, C., Fiorillo, G., Franceschi, A., Franco, D., Frolov, E., Funicello, N., Gabriele, F., Galbiati, C., Garbini, M., Abia, P. Garcia, Gendotti, A., Ghiano, C., Giampaolo, R. A., Giganti, C., Giorgi, M. A., Giovanetti, G. K., Gligan, M. L., Casanueva, V. Goicoechea, Gola, A., Goretti, A. M., Diaz, R. Graciani, Grigoriev, G. Y., Grobov, A., Gromov, M., Guan, M., Guerzoni, M., Guetti, M., Gulino, M., Guo, C., Hackett, B. R., Hallin, A., Haranczyk, M., Hill, S., Horikawa, S., Hubaut, F., Hugues, T., Hungerford, E. V., Ianni, An., Ippolito, V., James, C. C., Jillings, C., Kachru, P., Kemp, A. A., Kendziora, C. L., Keppel, G., Khomyakov, A. V., Kim, S., Kish, A., Kochanek, I., Kondo, K., Korga, G., Kubankin, A., Kugathasan, R., Kuss, M., Kuźniak, M., La Commara, M., La Delfa, L., La Grasta, D., Lai, M., Lami, N., Langrock, S., Leyton, M., Li, X., Lidey, L., Lippi, F., Lissia, M., Longo, G., Maccioni, N., Machulin, I. N., Mapelli, L., Marasciulli, A., Margotti, A., Mari, S. M., Maricic, J., Marinelli, M., Mart, M., Rojas, A. D. Martinez, Martini, A., Martoff, C. J., Mascia, M., Masetto, M., Masoni, A., Mazzi, A., McDonald, A. B., Mclaughlin, J., Messina, A., Meyers, P. D., Miletic, T., Milincic, R., Miola, R., Moggi, A., Moharana, A., Moioli, S., Monroe, J., Morisi, S., Morrocchi, M., Mozhevitina, E. N., Mr, T., Muratova, V. N., Murenu, A., Muscas, C., Musenich, L., Musico, P., Nania, R., Napolitano, T., Agasson, A. Navrer, Nessi, M., Nikulin, I., Nowak, J., Oleinik, A., Oleynikov, V., Pagani, L., Pallavicini, M., Palmas, S., Pandola, L., Pantic, E., Paoloni, E., Paternoster, G., Pegoraro, P. A., Pellegrini, L. A., Pellegrino, C., Pelczar, K., Perotti, F., Pesudo, V., Picciau, E., Pietropaolo, F., Pinna, T., Pocar, A., Podda, P., Poehlmann, D. M., Pordes, S., Poudel, S. S., Pralavorio, P., Price, D., Raffaelli, F., Ragusa, F., Ramirez, A., Razeti, M., Razeto, A., Renshaw, A. L., Rescia, S., Rescigno, M., Resnati, F., Retiere, F., Rignanese, L. P., Ripoli, C., Rivetti, A., Rode, J., Romero, L., Rossi, M., Rubbia, A., Rucaj, M., Sabiu, G. M., Salatino, P., Samoylov, O., S, E., Sandford, E., Sanfilippo, S., Sangiorgio, V. A., Santacroce, V., Santone, D., Santorelli, R., Santucci, A., Savarese, C., Scapparone, E., Schlitzer, B., Scioli, G., Semenov, D. A., Shaw, B., Shchagin, A., Sheshukov, A., Simeone, M., Skensved, P., Skorokhvatov, M. D., Smirnov, O., Smith, B., Sokolov, A., Stefanizzi, R., Steri, A., Stracka, S., Strickland, V., Stringer, M., Sulis, S., Suvorov, Y., Szelc, A. M., Zsücs-Balázs, J. Z., Tartaglia, R., Testera, G., Thorpe, T. N., Tonazzo, A., Torres-Lara, S., Tosti, S., Tricomi, A., Tuveri, M., Unzhakov, E. V., Usai, G., John, T. Vallivilayil, Vescovi, S., Viant, T., Viel, S., Vishneva, A., Vogelaar, R. B., Wada, M., Wang, H., Wang, Y., Westerdale, S., Wheadon, R. J., Williams, L., Wojcik, Ma. M., Wojcik, Ma., Xiao, X., Yang, C., Zani, A., Zenobio, F., Zichichi, A., Zuzel, G., and Zykova, M. P.

Subjects
Physics - Instrumentation and Detectors, Astrophysics - Instrumentation and Methods for Astrophysics
Abstract

The Aria project consists of a plant, hosting a 350 m cryogenic isotopic distillation column, the tallest ever built, which is currently in the installation phase in a mine shaft at Carbosulcis S.p.A., Nuraxi-Figus (SU), Italy. Aria is one of the pillars of the argon dark-matter search experimental program, lead by the Global Argon Dark Matter Collaboration. Aria was designed to reduce the isotopic abundance of $^{39}$Ar, a $\beta$-emitter of cosmogenic origin, whose activity poses background and pile-up concerns in the detectors, in the argon used for the dark-matter searches, the so-called Underground Argon (UAr). In this paper, we discuss the requirements, design, construction, tests, and projected performance of the plant for the isotopic cryogenic distillation of argon. We also present the successful results of isotopic cryogenic distillation of nitrogen with a prototype plant, operating the column at total reflux.

Published
2021
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6. Study of cosmogenic activation above ground for the DarkSide-20k experiment

Author

Elersich, A., Agnes, P., Ahmad, I., Albergo, S., Albuquerque, I.F.M., Alexander, T., Alton, A.K., Amaudruz, P., Atzori Corona, M., Ave, M., Avetisov, I.Ch., Azzolini, O., Back, H.O., Balmforth, Z., Barrado-Olmedo, A., Barrillon, P., Basco, A., Batignani, G., Bocci, V., Bonivento, W.M., Bottino, B., Boulay, M.G., Busto, J., Cadeddu, M., Caminata, A., Canci, N., Capra, A., Caprioli, S., Caravati, M., Cargioli, N., Carlini, M., Castello, P., Cavalcante, P., Cavuoti, S., Cebrian, S., Cela Ruiz, J.M., Chashin, S., Chepurnov, A., Chyhyrynets, E., Cifarelli, L., Cintas, D., Citterio, M., Cleveland, B., Cocco, V., Colaiuda, D., Conde Vilda, E., Consiglio, L., Copello, S., Covone, G., Czubak, M., D’Aniello, M., D’Auria, S., Da Rocha Rolo, M.D., Davini, S., De Cecco, S., De Gruttola, D., De Pasquale, S., De Rosa, G., Dellacasa, G., Derbin, A.V., Devoto, A., Di Capua, F., Di Noto, L., Di Stefano, P., Dolganov, G., Dordei, F., Ellingwood, E., Erjavec, T., Fernandez Diaz, M., Fiorillo, G., Franchini, P., Franco, D., Funicello, N., Gabriele, F., Gahan, D., Galbiati, C., Gallina, G., Gallus, G., Garbini, M., Garcia Abia, P., Gendotti, A., Ghiano, C., Giganti, C., Giovanetti, G.K., Goicoechea Casanueva, V., Gola, A., Grauso, G., Grilli di Cortona, G., Grobov, A., Gromov, M., Guan, M., Guerzoni, M., Gulino, M., Guo, C., Hackett, B.R., Hallin, A.L., Hamer, A., Haranczyk, M., Hessel, T., Hill, S., Horikawa, S., Hubaut, F., Hucker, J., Hugues, T., Ianni, An., Ippolito, V., Jillings, C., Jois, S., Kachru, P., Kemp, A.A., Kendziora, C.L., Kimura, M., Kochanek, I., Kondo, K., Korga, G., Koulosousas, S., Kubankin, A., Kuss, M., Kuzniak, M., La Commara, M., Lai, M., Le Guirriec, E., Leason, E., Leoni, A., Lidey, L., Lissia, M., Luzzi, L., Lychagina, O., Macfadyen, O., Machulin, I.N., Manecki, S., Manthos, I., Mapelli, L., Margotti, A., Mari, S.M., Mariani, C., Maricic, J., Marini, A., Martínez, M., Martoff, C.J., Matteucci, G., Mavrokoridis, K., McDonald, A.B., Messina, A., Milincic, R., Mitra, A., Moharana, A., Monroe, J., Moretti, E., Morrocchi, M., Mróz, T., Muratova, V.N., Muscas, C., Musico, P., Nania, R., Nessi, M., Nieradka, G., Nikolopoulos, K., Nowak, J., Olchansky, K., Oleinik, A., Oleynikov, V., Organtini, P., Ortiz de Solórzano, A., Pagani, L., Pallavicini, M., Pandola, L., Pantic, E., Paoloni, E., Paternoster, G., Pegoraro, P.A., Pelczar, K., Pellegrino, C., Pesudo, V., Piacentini, S., Pietrofaccia, L., Pino, N., Pocar, A., Poehlmann, D.M., Pordes, S., Pralavorio, P., Price, D., Ragusa, F., Ramachers, Y., Razeti, M., Renshaw, A.L., Rescigno, M., Retiere, F., Rignanese, L.P., Ripoli, C., Rivetti, A., Roberts, A., Roberts, C., Rode, J., Rogers, G., Romero, L., Rossi, M., Rubbia, A., Sabia, M.A., Salomone, P., Sandford, E., Sanfilippo, S., Santone, D., Santorelli, R., Savarese, C., Scapparone, E., Schillaci, G., Schuckman II, F.G., Scioli, G., Simeone, M., Skensved, P., Skorokhvatov, M.D., Smirnov, O., Smirnova, T., Smith, B., Spadoni, F., Spangenberg, M., Stefanizzi, R., Steri, A., Stornelli, V., Stracka, S., Stringer, M., Sulis, S., Sung, A., Suvorov, Y., Szelc, A.M., Tartaglia, R., Taylor, A., Taylor, J., Tedesco, S., Testera, G., Thieme, K., Thorpe, T.N., Tonazzo, A., Tricomi, A., Unzhakov, E.V., Vallivilayil John, T., Van Uffelen, M., Viant, T., Viel, S., Vogelaar, R.B., Vossebeld, J., Wada, M., Walczak, M.B., Wang, H., Wang, Y., Westerdale, S., Williams, L., Wingerter-Seez, I., Wojaczynski, R., Wojcik, Ma.M., Wright, T., Xie, Y., Yang, C., Zabihi, A., Zakhary, P., Zani, A., Zichichi, A., Zuzel, G., and Zykova, M.P.

Published
2023
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8. Measurement of isotopic separation of argon with the prototype of the cryogenic distillation plant Aria for dark matter searches

Author

Aaron, E., Agnes, P., Ahmad, I., Albergo, S., Albuquerque, I. F. M., Alexander, T., Alton, A. K., Amaudruz, P., Atzori Corona, M., Ave, M., Avetisov, I. Ch., Azzolini, O., Back, H. O., Balmforth, Z., Barrado, A., Barrillon, P., Basco, A., Batignani, G., Bocci, V., Bonivento, W. M., Bottino, B., Boulay, M. G., Busto, J., Cadeddu, M., Caminata, A., Canci, N., Capra, A., Caprioli, S., Caravati, M., Cargioli, N., Carlini, M., Castello, P., Cavalcante, P., Cavuoti, S., Cebrian, S., Cela Ruiz, J. M., Chashin, S., Chepurnov, A., Chyhyrynets, E., Cifarelli, L., Cintas, D., Citterio, M., Cleveland, B., Cocco, V., Conde Vilda, E., Consiglio, L., Copello, S., Covone, G., Czubak, M., D’Aniello, M., D’Auria, S., Da Rocha Rolo, M. D., Davini, S., De Cecco, S., De Guido, G., De Gruttola, D., De Pasquale, S., De Rosa, G., Dellacasa, G., Derbin, A. V., Devoto, A., Di Capua, F., Di Noto, L., Di Stefano, P., Dolganov, G., Dordei, F., Ellingwood, E., Erjavec, T., Farenzena, S., Fernandez Diaz, M., Fiorillo, G., Franchini, P., Franco, D., Funicello, N., Gabriele, F., Gahan, D., Galbiati, C., Gallina, G., Gallus, G., Garbini, M., Garcia Abia, P., Gendotti, A., Ghiano, C., Giganti, C., Giovanetti, G. K., Goicoechea Casanueva, V., Gola, A., Grauso, G., Grilli di Cortona, G., Grobov, A., Gromov, M., Guan, M., Guerzoni, M., Gulino, M., Guo, C., Hackett, B. R., Hallin, A. L., Hamer, A., Haranczyk, M., Hessel, T., Hill, S., Horikawa, S., Hubaut, F., Hucker, J., Hugues, T., Ianni, An., Ippolito, V., Jillings, C., Jois, S., Kachru, P., Kemp, A. A., Kendziora, C. L., Kimura, M., Kochanek, I., Kondo, K., Korga, G., Koulosousas, S., Kubankin, A., Kuss, M., Kuźniak, M., La Commara, M., Lai, M., Lami, N., Le Guirriec, E., Leason, E., Leoni, A., Lidey, L., Lippi, F., Lissia, M., Luzzi, L., Lychagina, O., Maccioni, N., Macfadyen, O., Machulin, I. N., Manecki, S., Manthos, I., Mapelli, L., Margotti, A., Mari, S. M., Mariani, C., Maricic, J., Marini, A., Martínez, M., Martoff, C. J., Mascia, M., Masoni, A., Matteucci, G., Mavrokoridis, K., Maxia, C., McDonald, A. B., Messina, A., Milincic, R., Mitra, A., Moharana, A., Moioli, S., Monroe, J., Moretti, E., Morrocchi, M., Mróz, T., Muratova, V. N., Muscas, C., Musico, P., Nania, R., Nessi, M., Nikolopoulos, K., Nowak, J., Olchansky, K., Oleinik, A., Oleynikov, V., Organtini, P., de Solórzano, A. Ortiz, Pagani, L., Pallavicini, M., Pandola, L., Pantic, E., Paoloni, E., Paternoster, G., Pegoraro, P. A., Pelczar, K., Pellegrini, L. A., Pellegrino, C., Pesudo, V., Piacentini, S., Pietrofaccia, L., Pino, N., Pocar, A., Poehlmann, D. M., Pordes, S., Pralavorio, P., Price, D., Ragusa, F., Ramachers, Y., Razeti, M., Renshaw, A. L., Rescigno, M., Retiere, F., Rignanese, L. P., Ripoli, C., Rivetti, A., Roberts, A., Roberts, C., Rode, J., Rogers, G., Romero, L., Rossi, M., Rubbia, A., Sabia, M. A., Sabiu, G. M., Salomone, P., Sandford, E., Sanfilippo, S., Santone, D., Santorelli, R., Savarese, C., Scapparone, E., Schillaci, G., Schukman, F., Scioli, G., Simeone, M., Skensved, P., Skorokhvatov, M. D., Smirnov, O., Smirnova, T., Smith, B., Spadoni, F., Spangenberg, M., Stefanizzi, R., Steri, A., Stornelli, V., Stracka, S., Stringer, M., Sulis, S., Sung, A., Suvorov, Y., Szelc, A. M., Tartaglia, R., Taylor, A., Taylor, J., Tedesco, S., Testera, G., Thieme, K., Thorpe, T. N., Tonazzo, A., Tricomi, A., Unzhakov, E. V., Vallivilayil John, T., Van Uffelen, M., Viant, T., Viel, S., Vogelaar, R. B., Vossebeld, J., Wada, M., Walczak, M. B., Wang, H., Wang, Y., Westerdale, S., Williams, L., Wingerter-Seez, I., Wojaczyński, R., Wojcik, Ma. M., Wright, T., Xie, Y., Yang, C., Zabihi, A., Zakhary, P., Zani, A., Zichichi, A., Zuzel, G., and Zykova, M. P.

Published
2023
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11. Strengthening of RC Buildings with Steel Elements

Author

Castro, J. M., Araújo, M., D’Aniello, M., Landolfo, R., de Freitas, Vasco Peixoto, Series editor, Costa, Anibal, Series editor, Delgado, João M.P.Q., Series editor, Costa, Aníbal, editor, Arêde, António, editor, and Varum, Humberto, editor

Published
2018
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13. The NAC family transcription factor GmNAC42–1 regulates biosynthesis of the anticancer and neuroprotective glyceollins in soybean

Author

Md Asraful Jahan, Brianna Harris, Matthew Lowery, Katie Coburn, Aniello M. Infante, Ryan J. Percifield, Amanda G. Ammer, and Nik Kovinich

Subjects
Phytoalexin, Transcription factor, NAC, Isoflavonoids, Glyceollins, Biotechnology, TP248.13-248.65, Genetics, QH426-470
Abstract

Abstract Background Glyceollins are isoflavonoid-derived pathogen-inducible defense metabolites (phytoalexins) from soybean (Glycine max L. Merr) that have important roles in providing defense against pathogens. They also have impressive anticancer and neuroprotective activities in mammals. Despite their potential usefulness as therapeutics, glyceollins are not economical to synthesize and are biosynthesized only transiently and in low amounts in response to specific stresses. Engineering the regulation of glyceollin biosynthesis may be a promising approach to enhance their bioproduction, yet the transcription factors (TFs) that regulate their biosynthesis have remained elusive. To address this, we first aimed to identify novel abiotic stresses that enhance or suppress the elicitation of glyceollins and then used a comparative transcriptomics approach to search for TF gene candidates that may positively regulate glyceollin biosynthesis. Results Acidity stress (pH 3.0 medium) and dehydration exerted prolonged (week-long) inductive or suppressive effects on glyceollin biosynthesis, respectively. RNA-seq found that all known biosynthetic genes were oppositely regulated by acidity stress and dehydration, but known isoflavonoid TFs were not. Systemic acquired resistance (SAR) genes were highly enriched in the geneset. We chose to functionally characterize the NAC (NAM/ATAF1/2/CUC2)-family TF GmNAC42–1 that was annotated as an SAR gene and a homolog of the Arabidopsis thaliana (Arabidopsis) indole alkaloid phytoalexin regulator ANAC042. Overexpressing and silencing GmNAC42–1 in elicited soybean hairy roots dramatically enhanced and suppressed the amounts of glyceollin metabolites and biosynthesis gene mRNAs, respectively. Yet, overexpressing GmNAC42–1 in non-elicited hairy roots failed to stimulate the expressions of all biosynthesis genes. Thus, GmNAC42–1 was necessary but not sufficient to activate all biosynthesis genes on its own, suggesting an important role in the glyceollin gene regulatory network (GRN). The GmNAC42–1 protein directly bound the promoters of biosynthesis genes IFS2 and G4DT in the yeast one-hybrid (Y1H) system. Conclusions Acidity stress is a novel elicitor and dehydration is a suppressor of glyceollin biosynthesis. The TF gene GmNAC42–1 is an essential positive regulator of glyceollin biosynthesis. Overexpressing GmNAC42–1 in hairy roots can be used to increase glyceollin yields > 10-fold upon elicitation. Thus, manipulating the expressions of glyceollin TFs is an effective strategy for enhancing the bioproduction of glyceollins in soybean.

Published
2019
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14. Site-Dependent Lineage Preference of Adipose Stem Cells

Author

Tingliang Wang, Ryan C. Hill, Monika Dzieciatkowska, Lian Zhu, Aniello M. Infante, Gangqing Hu, Kirk C. Hansen, and Ming Pei

Subjects
adipogenesis, adipose stem cell, chondrogenesis, infrapatellar fat pad, osteogenesis, subcutaneous adipose tissue, Biology (General), QH301-705.5
Abstract

Adult stem cells have unique properties in both proliferation and differentiation preference. In this study, we hypothesized that adipose stem cells have a depot-dependent lineage preference. Four rabbits were used to provide donor-matched adipose stem cells from either subcutaneous adipose tissue (ScAT) or infrapatellar fat pad (IPFP). Proliferation and multi-lineage differentiation were evaluated in adipose stem cells from donor-matched ScAT and IPFP. RNA sequencing (RNA-seq) and proteomics were conducted to uncover potential molecular discrepancy in adipose stem cells and their corresponding matrix microenvironments. We found that stem cells from ScAT exhibited significantly higher proliferation and adipogenic capacity compared to those from donor-matched IPFP while stem cells from IPFP displayed significantly higher chondrogenic potential compared to those from donor-matched ScAT. Our findings are strongly endorsed by supportive data from transcriptome and proteomics analyses, indicating a site-dependent lineage preference of adipose stem cells.

Published
2020
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15. Dysregulation of metabolic‐associated pathways in muscle of breast cancer patients: preclinical evaluation of interleukin‐15 targeting fatigue

Author

Joseph Bohlen, Sarah L. McLaughlin, Hannah Hazard‐Jenkins, Aniello M. Infante, Cortney Montgomery, Mary Davis, and Emidio E. Pistilli

Subjects
Cachexia, Breast cancer, Fatigue, Transcriptome, IL‐15, Diseases of the musculoskeletal system, RC925-935, Human anatomy, QM1-695
Abstract

Abstract Background Breast cancer patients report a perception of increased muscle fatigue, which can persist following surgery and standardized therapies. In a clinical experiment, we tested the hypothesis that pathways regulating skeletal muscle fatigue are down‐regulated in skeletal muscle of breast cancer patients and that different muscle gene expression patterns exist between breast tumour subtypes. In a preclinical study, we tested the hypothesis that mammary tumour growth in mice induces skeletal muscle fatigue and that overexpression of the cytokine interleukin‐15 (IL‐15) can attenuate mammary tumour‐induced muscle fatigue. Methods Early stage non‐metastatic female breast cancer patients (n = 14) and female non‐cancer patients (n = 6) provided a muscle biopsy of the pectoralis major muscle during mastectomy, lumpectomy, or breast reconstruction surgeries. The breast cancer patients were diagnosed with either luminal (ER+/PR+, n = 6), triple positive (ER+/PR+/Her2/neu+, n = 5), or triple negative (ER−/PR−/Her2/neu−, n = 3) breast tumours and were being treated with curative intent either with neoadjuvant chemotherapy followed by surgery or surgery followed by standard post‐operative therapy. Biopsies were used for RNA‐sequencing to compare the skeletal muscle gene expression patterns between breast cancer patients and non‐cancer patients. The C57BL/6 mouse syngeneic mammary tumour cell line, E0771, was used to induce mammary tumours in immunocompetent mice, and isometric muscle contractile properties and fatigue properties were analysed following 4 weeks of tumour growth. Results RNA‐sequencing and subsequent bioinformatics analyses revealed a dysregulation of canonical pathways involved in oxidative phosphorylation, mitochondrial dysfunction, peroxisome proliferator‐activated receptor signalling and activation, and IL‐15 signalling and production. In a preclinical mouse model of breast cancer, the rate of muscle fatigue was greater in mice exposed to mammary tumour growth for 4 weeks, and this greater muscle fatigue was attenuated in transgenic mice that overexpressed the cytokine IL‐15. Conclusions Our data identify novel genes and pathways dysregulated in the muscles of breast cancer patients with early stage non‐metastatic disease, with particularly aberrant expression among genes that would predispose these patients to greater muscle fatigue. Furthermore, we demonstrate that IL‐15 overexpression can attenuate muscle fatigue associated with mammary tumour growth in a preclinical mouse model of breast cancer. Therefore, we propose that skeletal muscle fatigue is an inherent consequence of breast tumour growth, and this greater fatigue can be targeted therapeutically.

Published
2018
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16. Genome-wide interrogation of hepatic FXR reveals an asymmetric IR-1 motif and synergy with LRH-1

Author

Chong, Hansook Kim, Infante, Aniello M., Seo, Young-Kyo, Jeon, Tae-Il, Zhang, Yanqiao, Edwards, Peter A., Xie, Xiaohui, and Osborne, Timothy F.

Subjects
farnesoid-x-receptor, orphan nuclear receptor, fatty-acid synthase, ppar-gamma-rxr, bile-acid, activated receptor, target genes, binding, liver, dna
Abstract

We used mouse hepatic chromatin enriched with an FXR antibody and chromatin immunoprecipitation-sequencing (ChIP-seq) to evaluate FXR binding on a genome-wide scale. This identified 1656 FXR-binding sites and 10% were located within 2 kb of a transcription start site which is much higher than predicted by random occurrence. A motif search uncovered a canonical nuclear receptor IR-1 site, consistent with in vitro DNA-binding studies reported previously. A separate nuclear receptor half-site for monomeric receptors such as LRH-1 was co-enriched and FXR activation of four newly identified promoters was significantly augmented by an LRH-1 expression vector in a co-transfection assay. There were 1038 genes located within 20 kb of a peak and a gene set enrichment analysis showed that genes identified by our ChIP-seq analysis are highly correlated with genes activated by an FXR-VP16 adenovirus in primary mouse hepatocytes providing functional relevance to the genome-wide binding study. Gene Ontology analysis showed FXR-binding sites close to many genes in lipid, fatty acid and steroid metabolism. Other broad gene clusters related to metabolism, transport, signaling and glycolysis were also significantly enriched. Thus, FXR may have a much wider role in cellular metabolism than previously appreciated.

Published
2010

18. The European Physical Journal C

Author

Aaron, E., Agnes, P., Ahmad, I., Albergo, S., Albuquerque, I. F. M., Alexander, T., Alton, A. K., Amaudruz, P., Atzori Corona, M., Ave, M., Avetisov, I. Ch., Azzolini, O., Back, H. O., Balmforth, Z., Barrado, A., Barrillon, P., Basco, A., Batignani, G., Bocci, V., Bonivento, W. M., Bottino, B., Boulay, M. G., Busto, J., Cadeddu, M., Caminata, A., Canci, N., Capra, A., Caprioli, S., Caravati, M., Cargioli, N., Carlini, M., Castello, P., Cavalcante, P., Cavuoti, S., Cebrian, S., Cela Ruiz, J. M., Chashin, S., Chepurnov, A., Chyhyrynets, E., Cifarelli, L., Cintas, D., Citterio, M., Cleveland, B., Cocco, V., Conde Vilda, E., Consiglio, L., Copello, S., Covone, G., Czubak, M., D’Aniello, M., D’Auria, S., Da Rocha Rolo, M. D., Davini, S., De Cecco, S., De Guido, G., De Gruttola, D., De Pasquale, S., De Rosa, G., Dellacasa, G., Derbin, A. V., Devoto, A., Di Capua, F., Di Noto, L., Di Stefano, P., Dolganov, G., Dordei, F., Ellingwood, E., Erjavec, T., Farenzena, S., Fernandez Diaz, M., Fiorillo, G., Franchini, P., Franco, D., Funicello, N., Gabriele, F., Gahan, D., Galbiati, C., Gallina, G., Gallus, G., Garbini, M., Garcia Abia, P., Gendotti, A., Ghiano, C., Giganti, C., Giovanetti, G. K., Goicoechea Casanueva, V., Gola, A., Grauso, G., Grilli di Cortona, G., Grobov, A., Gromov, M., Guan, M., Guerzoni, M., Gulino, M., Guo, C., Hackett, B. R., Hallin, A. L., Hamer, A., Haranczyk, M., Hessel, T., Hill, S., Horikawa, S., Hubaut, F., Hucker, J., Hugues, T., Ianni, An., Ippolito, V., Jillings, C., Jois, S., Kachru, P., Kemp, A. A., Kendziora, C. L., Kimura, M., Kochanek, I., Kondo, K., Korga, G., Koulosousas, S., Kubankin, A., Kuss, M., Kuźniak, M., La Commara, M., Lai, M., Lami, N., Le Guirriec, E., Leason, E., Leoni, A., Lidey, L., Lippi, F., Lissia, M., Luzzi, L., Lychagina, O., Maccioni, N., Macfadyen, O., Machulin, I. N., Manecki, S., Manthos, I., Mapelli, L., Margotti, A., Mari, S. M., Mariani, C., Maricic, J., Marini, A., Martínez, M., Martoff, C. J., Mascia, M., Masoni, A., Matteucci, G., Mavrokoridis, K., Maxia, C., Mcdonald, A. B., Messina, A., Milincic, R., Mitra, A., Moharana, A., Moioli, S., Monroe, J., Moretti, E., Morrocchi, M., Mróz, T., Muratova, V. N., Muscas, C., Musico, P., Nania, R., Nessi, M., Nikolopoulos, K., Nowak, J., Olchansky, K., Oleinik, A., Oleynikov, V., Organtini, P., Ortiz de Solórzano, A., Pagani, L., Pallavicini, M., Pandola, L., Pantic, E., Paoloni, E., Paternoster, G., Pegoraro, P. A., Pelczar, K., Pellegrini, L. A., Pellegrino, C., Pesudo, V., Piacentini, S., Pietrofaccia, L., Pino, N., Pocar, A., Poehlmann, D. M., Pordes, S., Pralavorio, P., Price, D., Ragusa, F., Ramachers, Y., Razeti, M., Renshaw, A. L., Rescigno, M., Retiere, F., Rignanese, L. P., Ripoli, C., Rivetti, A., Roberts, A., Roberts, C., Rode, J., Rogers, G., Romero, L., Rossi, M., Rubbia, A., Sabia, M. A., Sabiu, G. M., Salomone, P., Sandford, E., Sanfilippo, S., Santone, D., Santorelli, R., Savarese, C., Scapparone, E., Schillaci, G., Schukman, F., Scioli, G., Simeone, M., Skensved, P., Skorokhvatov, M. D., Smirnov, O., Smirnova, T., Smith, B., Spadoni, F., Spangenberg, M., Stefanizzi, R., Steri, A., Stornelli, V., Stracka, S., Stringer, M., Sulis, S., Sung, A., Suvorov, Y., Szelc, A. M., Tartaglia, R., Taylor, A., Taylor, J., Tedesco, S., Testera, G., Thieme, K., Thorpe, T. N., Tonazzo, A., Tricomi, A., Unzhakov, E. V., Vallivilayil John, T., Van Uffelen, M., Viant, T., Viel, S., Vogelaar, R. B., Vossebeld, J., Wada, M., Walczak, M. B., Wang, H., Wang, Y., Westerdale, S., Williams, L., Wingerter-Seez, I., Wojaczyński, R., Wojcik, Ma. M., Wright, T., Xie, Y., Yang, C., Zabihi, A., Zakhary, P., Zani, A., Zichichi, A., Zuzel, G., Zykova &amp, M. P., DarkSide-20k, Collaboration, Centre de Physique des Particules de Marseille (CPPM), Aix Marseille Université (AMU)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), AstroParticule et Cosmologie (APC (UMR_7164)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Observatoire de Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Laboratoire de Physique Nucléaire et de Hautes Énergies (LPNHE (UMR_7585)), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), DarkSide-20k, Aaron, E., Agnes, P., Ahmad, I., Albergo, S., Albuquerque, I. F. M., Alexander, T., Alton, A. K., Amaudruz, P., Atzori Corona, M., Ave, M., Avetisov, I. C., Azzolini, O., Back, H. O., Balmforth, Z., Barrado, A., Barrillon, P., Basco, A., Batignani, G., Bocci, V., Bonivento, W. M., Bottino, B., Boulay, M. G., Busto, J., Cadeddu, M., Caminata, A., Canci, N., Capra, A., Caprioli, S., Caravati, M., Cargioli, N., Carlini, M., Castello, P., Cavalcante, P., Cavuoti, S., Cebrian, S., Cela Ruiz, J. M., Chashin, S., Chepurnov, A., Chyhyrynets, E., Cifarelli, L., Cintas, D., Citterio, M., Cleveland, B., Cocco, V., Conde Vilda, E., Consiglio, L., Copello, S., Covone, G., Czubak, M., D'Aniello, M., D'Auria, S., Da Rocha Rolo, M. D., Davini, S., De Cecco, S., De Guido, G., De Gruttola, D., De Pasquale, S., De Rosa, G., Dellacasa, G., Derbin, A. V., Devoto, A., Di Capua, F., Di Noto, L., Di Stefano, P., Dolganov, G., Dordei, F., Ellingwood, E., Erjavec, T., Farenzena, S., Fernandez Diaz, M., Fiorillo, G., Franchini, P., Franco, D., Funicello, N., Gabriele, F., Gahan, D., Galbiati, C., Gallina, G., Gallus, G., Garbini, M., Garcia Abia, P., Gendotti, A., Ghiano, C., Giganti, C., Giovanetti, G. K., Goicoechea Casanueva, V., Gola, A., Grauso, G., Grilli di Cortona, G., Grobov, A., Gromov, M., Guan, M., Guerzoni, M., Gulino, M., Guo, C., Hackett, B. R., Hallin, A. L., Hamer, A., Haranczyk, M., Hessel, T., Hill, S., Horikawa, S., Hubaut, F., Hucker, J., Hugues, T., Ianni, A., Ippolito, V., Jillings, C., Jois, S., Kachru, P., Kemp, A. A., Kendziora, C. L., Kimura, M., Kochanek, I., Kondo, K., Korga, G., Koulosousas, S., Kubankin, A., Kuss, M., Kuzniak, M., La Commara, M., Lai, M., Lami, N., Le Guirriec, E., Leason, E., Leoni, A., Lidey, L., Lippi, F., Lissia, M., Luzzi, L., Lychagina, O., Maccioni, N., Macfadyen, O., Machulin, I. N., Manecki, S., Manthos, I., Mapelli, L., Margotti, A., Mari, S. M., Mariani, C., Maricic, J., Marini, A., Martinez, M., Martoff, C. J., Mascia, M., Masoni, A., Matteucci, G., Mavrokoridis, K., Maxia, C., Mcdonald, A. B., Messina, A., Milincic, R., Mitra, A., Moharana, A., Moioli, S., Monroe, J., Moretti, E., Morrocchi, M., Mroz, T., Muratova, V. N., Muscas, C., Musico, P., Nania, R., Nessi, M., Nikolopoulos, K., Nowak, J., Olchansky, K., Oleinik, A., Oleynikov, V., Organtini, P., de Solorzano, A. O., Pagani, L., Pallavicini, M., Pandola, L., Pantic, E., Paoloni, E., Paternoster, G., Pegoraro, P. A., Pelczar, K., Pellegrini, L. A., Pellegrino, C., Pesudo, V., Piacentini, S., Pietrofaccia, L., Pino, N., Pocar, A., Poehlmann, D. M., Pordes, S., Pralavorio, P., Price, D., Ragusa, F., Ramachers, Y., Razeti, M., Renshaw, A. L., Rescigno, M., Retiere, F., Rignanese, L. P., Ripoli, C., Rivetti, A., Roberts, A., Roberts, C., Rode, J., Rogers, G., Romero, L., Rossi, M., Rubbia, A., Sabia, M. A., Sabiu, G. M., Salomone, P., Sandford, E., Sanfilippo, S., Santone, D., Santorelli, R., Savarese, C., Scapparone, E., Schillaci, G., Schukman, F., Scioli, G., Simeone, M., Skensved, P., Skorokhvatov, M. D., Smirnov, O., Smirnova, T., Smith, B., Spadoni, F., Spangenberg, M., Stefanizzi, R., Steri, A., Stornelli, V., Stracka, S., Stringer, M., Sulis, S., Sung, A., Suvorov, Y., Szelc, A. M., Tartaglia, R., Taylor, A., Taylor, J., Tedesco, S., Testera, G., Thieme, K., Thorpe, T. N., Tonazzo, A., Tricomi, A., Unzhakov, E. V., Vallivilayil John, T., Van Uffelen, M., Viant, T., Viel, S., Vogelaar, R. B., Vossebeld, J., Wada, M., Walczak, M. B., Wang, H., Wang, Y., Westerdale, S., Williams, L., Wingerter-Seez, I., Wojaczynski, R., Wojcik, M. M., Wright, T., Xie, Y., Yang, C., Zabihi, A., Zakhary, P., Zani, A., Zichichi, A., Zuzel, G., and Zykova, M. P.

Subjects
Physics - Instrumentation and Detectors, WIMP, dark matter, DarkSide-20k, stability, buildings, programming, nitrogen, cryogenic distillation, Gran Sasso, astro-ph.IM, cryogenics, quality, Dark Matter, Argon, [PHYS.PHYS.PHYS-INS-DET]Physics [physics]/Physics [physics]/Instrumentation and Detectors [physics.ins-det], Astrophysics - Instrumentation and Methods for Astrophysics, experimental results
Abstract

The Aria cryogenic distillation plant, located in Sardinia, Italy, is a key component of the DarkSide-20k experimental program for WIMP dark matter searches at the INFN Laboratori Nazionali del Gran Sasso, Italy. Aria is designed to purify the argon, extracted from underground wells in Colorado, USA, and used as the DarkSide-20k target material, to detector-grade quality. In this paper, we report the first measurement of argon isotopic separation by distillation with the 26 m tall Aria prototype. We discuss the measurement of the operating parameters of the column and the observation of the simultaneous separation of the three stable argon isotopes: $${}^{36}\hbox {Ar}$$ 36 Ar , $${}^{38}\textrm{Ar}$$ 38 Ar , and $${}^{40}\textrm{Ar}$$ 40 Ar . We also provide a detailed comparison of the experimental results with commercial process simulation software. This measurement of isotopic separation of argon is a significant achievement for the project, building on the success of the initial demonstration of isotopic separation of nitrogen using the same equipment in 2019.

Published
2023
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19. Figure S1 from Human Breast Cancer Xenograft Model Implicates Peroxisome Proliferator–activated Receptor Signaling as Driver of Cancer-induced Muscle Fatigue

Author

Wilson, Hannah E., primary, Rhodes, Kacey K., primary, Rodriguez, Daniel, primary, Chahal, Ikttesh, primary, Stanton, David A., primary, Bohlen, Joseph, primary, Davis, Mary, primary, Infante, Aniello M., primary, Hazard-Jenkins, Hannah, primary, Klinke, David J., primary, Pugacheva, Elena N., primary, and Pistilli, Emidio E., primary

Published
2023
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20. Table S3 from Human Breast Cancer Xenograft Model Implicates Peroxisome Proliferator–activated Receptor Signaling as Driver of Cancer-induced Muscle Fatigue

Author

Wilson, Hannah E., primary, Rhodes, Kacey K., primary, Rodriguez, Daniel, primary, Chahal, Ikttesh, primary, Stanton, David A., primary, Bohlen, Joseph, primary, Davis, Mary, primary, Infante, Aniello M., primary, Hazard-Jenkins, Hannah, primary, Klinke, David J., primary, Pugacheva, Elena N., primary, and Pistilli, Emidio E., primary

Published
2023
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21. Data from Human Breast Cancer Xenograft Model Implicates Peroxisome Proliferator–activated Receptor Signaling as Driver of Cancer-induced Muscle Fatigue

Author

Emidio E. Pistilli, Elena N. Pugacheva, David J. Klinke, Hannah Hazard-Jenkins, Aniello M. Infante, Mary Davis, Joseph Bohlen, David A. Stanton, Ikttesh Chahal, Daniel Rodriguez, Kacey K. Rhodes, and Hannah E. Wilson

Abstract

Purpose:This study tested the hypothesis that a patient-derived orthotopic xenograft (PDOX) model would recapitulate the common clinical phenomenon of breast cancer–induced skeletal muscle (SkM) fatigue in the absence of muscle wasting. This study additionally sought to identify drivers of this condition to facilitate the development of therapeutic agents for patients with breast cancer experiencing muscle fatigue.Experimental Design:Eight female BC-PDOX–bearing mice were produced via transplantation of tumor tissue from 8 female patients with breast cancer. Individual hind limb muscles from BC-PDOX mice were isolated at euthanasia for RNA-sequencing, gene and protein analyses, and an ex vivo muscle contraction protocol to quantify tumor-induced aberrations in SkM function. Differentially expressed genes (DEG) in the BC-PDOX mice relative to control mice were identified using DESeq2, and multiple bioinformatics platforms were employed to contextualize the DEGs.Results:We found that SkM from BC-PDOX–bearing mice showed greater fatigability than control mice, despite no differences in absolute muscle mass. PPAR, mTOR, IL6, IL1, and several other signaling pathways were implicated in the transcriptional changes observed in the BC-PDOX SkM. Moreover, 3 independent in silico analyses identified PPAR signaling as highly dysregulated in the SkM of both BC-PDOX–bearing mice and human patients with early-stage nonmetastatic breast cancer.Conclusions:Collectively, these data demonstrate that the BC-PDOX model recapitulates the expected breast cancer–induced SkM fatigue and further identify aberrant PPAR signaling as an integral factor in the pathology of this condition.

Published
2023
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22. Table S3 from Human Breast Cancer Xenograft Model Implicates Peroxisome Proliferator–activated Receptor Signaling as Driver of Cancer-induced Muscle Fatigue

Author

Emidio E. Pistilli, Elena N. Pugacheva, David J. Klinke, Hannah Hazard-Jenkins, Aniello M. Infante, Mary Davis, Joseph Bohlen, David A. Stanton, Ikttesh Chahal, Daniel Rodriguez, Kacey K. Rhodes, and Hannah E. Wilson

Abstract

Table S3 shows data for correlational analyses between muscle contractile parameters and STAT activation

Published
2023
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23. Figure S1 from Human Breast Cancer Xenograft Model Implicates Peroxisome Proliferator–activated Receptor Signaling as Driver of Cancer-induced Muscle Fatigue

Author

Emidio E. Pistilli, Elena N. Pugacheva, David J. Klinke, Hannah Hazard-Jenkins, Aniello M. Infante, Mary Davis, Joseph Bohlen, David A. Stanton, Ikttesh Chahal, Daniel Rodriguez, Kacey K. Rhodes, and Hannah E. Wilson

Abstract

Figure S1 shows additional data from Enrichr in silico analyses, and protein analyses of STAT 3 and STAT5.

Published
2023
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27. DUAL STEEL ECCENTRICALLY BRACED FRAMES DESIGNED ACCORDING TO EUROCODE 8

Author

Costanzo S., D'Aniello M., Landolfo R., Costanzo, S., D'Aniello, M., and Landolfo, R.

Subjects
Eurocode 8, High strength steel, Dual steel, Seismic design, Eccentrically braced frame
Abstract

The use of high strength (HS) steel in seismic applications is very attractive due to economical and mechanical benefits. The combined use of HSS for non-dissipative members and MCS for dissipative zones is generally termed “dual-steel” concept and it could likely represent a valuable aid to satisfy capacity design criteria and to control the global frame behaviour, even contemporarily reducing the constructional cost. The current paper investigates the benefits of applying dual-steel concept to the seismic design of eccentrically braced frames equipped with short links in the framework of EN 1998-1:2005. With this purpose, a comprehensive numerical parametric study has been carried out: both static and dynamic nonlinear analyses have been performed to investigate the advantages of using HSS to enhance strength and ductility of a set of mid and high-rise simple and dual eccentrically braced frames.

Published
2022

29. Separating $^{39}$Ar from $^{40}$Ar by cryogenic distillation with Aria for dark matter searches

Author

Agnes, P., Albergo, S., Albuquerque, I.F.M., Alexander, T., Alici, A., Alton, A.K., Amaudruz, P., Arba, M., Arpaia, P., Arcelli, S., Ave, M., Avetissov, I.Ch., Avetisov, R.I., Azzolini, O., Back, H.O., Balmforth, Z., Barbarian, V., Olmedo, A. Barrado, Barrillon, P., Basco, A., Batignani, G., Bondar, A., Bonivento, W.M., Borisova, E., Bottino, B., Boulay, M.G., Buccino, G., Bussino, S., Busto, J., Buzulutskov, A., Cadeddu, M., Cadoni, M., Caminata, A., Canesi, E.V., Canci, N., Cappello, G., Caravati, M., Cárdenas-Montes, M., Cargioli, N., Carlini, M., Carnesecchi, F., Castello, P., Castellani, A., Catalanotti, S., Cataudella, V., Cavalcante, P., Cavuoti, S., Cebrian, S., Cela Ruiz, J.M., Celano, B., Chashin, S., Chepurnov, A., Cicalò, C., Cifarelli, L., Cintas, D., Coccetti, F., Cocco, V., Colocci, M., Vilda, E. Conde, Consiglio, L., Copello, S., Corning, J., Covone, G., Czudak, P., d'Aniello, M., d'Auria, S., da Rocha Rolo, M., Dadoun, O., Daniel, M., Davini, S., de Candia, A., De Cecco, S., De Falco, A., de Filippis, G., De Gruttola, D., De Guido, G., De Rosa, G., Della Valle, M., Dellacasa, G., De Pasquale, S., Derbin, A.V., Devoto, A., Di Noto, L., Di Eusanio, F., Dionisi, C., Di Stefano, P., Dolganov, G., Dongiovanni, D., Dordei, F., Downing, M., Erjavec, T., Falciano, S., Farenzena, S., Diaz, M. Fernandez, Filip, C., Fiorillo, G., Franceschi, A., Franco, D., Frolov, E., Funicello, N., Gabriele, F., Galbiati, C., Garbini, M., Abia, P. Garcia, Gendotti, A., Ghiano, C., Giampaolo, R.A., Giganti, C., Giorgi, M.A., Giovanetti, G.K., Gligan, M.L., Casanueva, V. Goicoechea, Gola, A., Goretti, A.M., Graciani Diaz, R., Grigoriev, G.Y., Grobov, A., Gromov, M., Guan, M., Guerzoni, M., Guetti, M., Gulino, M., Guo, C., Hackett, B.R., Hallin, A., Haranczyk, M., Hill, S., Horikawa, S., Hubaut, F., Hugues, T., Hungerford, E.V., Ianni, An., Ippolito, V., James, C.C., Jillings, C., Kachru, P., Kemp, A.A., Kendziora, C.L., Keppel, G., Khomyakov, A.V., Kim, S., Kish, A., Kochanek, I., Kondo, K., Korga, G., Kubankin, A., Kugathasan, R., Kuss, M., Kuźniak, M., La Commara, M., La Delfa, L., La Grasta, D., Lai, M., Lami, N., Langrock, S., Leyton, M., Li, X., Lidey, L., Lippi, F., Lissia, M., Longo, G., Maccioni, N., Machulin, I.N., Mapelli, L., Marasciulli, A., Margotti, A., Mari, S.M., Maricic, J., Marinelli, M., Martínez, M., Rojas, A.D. Martinez, Martini, A., Martoff, C.J., Mascia, M., Masetto, M., Masoni, A., Mazzi, A., Mcdonald, A.B., Mclaughlin, J., Messina, A., Meyers, P.D., Miletic, T., Milincic, R., Miola, R., Moggi, A., Moharana, A., Moioli, S., Monroe, J., Morisi, S., Morrocchi, M., Mozhevitina, E.N., Mr, T., Mróz, T., Muratova, V.N., Murenu, A., Muscas, C., Musenich, L., Musico, P., Nania, R., Napolitano, T., Navrer Agasson, A., Nessi, M., Nikulin, I., Nowak, J., Oleinik, A., Oleynikov, V., Pagani, L., Pallavicini, M., Palmas, S., Pandola, L., Pantic, E., Paoloni, E., Paternoster, G., Pegoraro, P.A., Pellegrini, L.A., Pellegrino, C., Pelczar, K., Perotti, F., Pesudo, V., Picciau, E., Pietropaolo, F., Pinna, T., Pocar, A., Podda, P., Poehlmann, D.M., Pordes, S., Poudel, S.S., Pralavorio, P., Price, D., Raffaelli, F., Ragusa, F., Ramirez, A., Razeti, M., Razeto, A., Renshaw, A.L., Rescia, S., Rescigno, M., Resnati, F., Retiere, F., Rignanese, L.P., Ripoli, C., Rivetti, A., Rode, J., Romero, L., Rossi, M., Rubbia, A., Rucaj, M., Sabiu, G.M., Salatino, P., Samoylov, O., Sánchez García, E., Sandford, E., Sanfilippo, S., Sangiorgio, V.A., Santacroce, V., Santone, D., Santorelli, R., Santucci, A., Savarese, C., Scapparone, E., Schlitzer, B., Scioli, G., Semenov, D.A., Shaw, B., Shchagin, A., Sheshukov, A., Simeone, M., Skensved, P., Skorokhvatov, M.D., Smirnov, O., Smith, B., Sokolov, A., Stefanizzi, R., Steri, A., Stracka, S., Strickland, V., Stringer, M., Sulis, S., Suvorov, Y., Szelc, A.M., Zsücs-Balázs, J.Z., Tartaglia, R., Testera, G., Thorpe, T.N., Tonazzo, A., Torres-Lara, S., Tosti, S., Tricomi, A., Tuveri, M., Unzhakov, E.V., Usai, G., John, T. Vallivilayil, Vescovi, S., Viant, T., Viel, S., Vishneva, A., Vogelaar, R.B., Wada, M., Wang, H., Wang, Y., Westerdale, S., Wheadon, R.J., Williams, L., Wojcik, Ma.M., Wojcik, Ma., Xiao, X., Yang, C., Zani, A., Zenobio, F., Zichichi, A., Zuzel, G., Zykova, M.P., Agnes P., Albergo S., Albuquerque I.F.M., Alexander T., Alici A., Alton A.K., Amaudruz P., Arba M., Arpaia P., Arcelli S., Ave M., Avetissov I.C., Avetisov R.I., Azzolini O., Back H.O., Balmforth Z., Barbarian V., Barrado Olmedo A., Barrillon P., Basco A., Batignani G., Bondar A., Bonivento W.M., Borisova E., Bottino B., Boulay M.G., Buccino G., Bussino S., Busto J., Buzulutskov A., Cadeddu M., Cadoni M., Caminata A., Canesi E.V., Canci N., Cappello G., Caravati M., Cardenas-Montes M., Cargioli N., Carlini M., Carnesecchi F., Castello P., Castellani A., Catalanotti S., Cataudella V., Cavalcante P., Cavuoti S., Cebrian S., Cela Ruiz J.M., Celano B., Chashin S., Chepurnov A., Cicalo C., Cifarelli L., Cintas D., Coccetti F., Cocco V., Colocci M., Conde Vilda E., Consiglio L., Copello S., Corning J., Covone G., Czudak P., D'Aniello M., D'Auria S., Da Rocha Rolo M.D., Dadoun O., Daniel M., Davini S., De Candia A., De Cecco S., De Falco A., De Filippis G., De Gruttola D., De Guido G., De Rosa G., Della Valle M., Dellacasa G., De Pasquale S., Derbin A.V., Devoto A., Di Noto L., Di Eusanio F., Dionisi C., Di Stefano P., Dolganov G., Dongiovanni D., Dordei F., Downing M., Erjavec T., Falciano S., Farenzena S., Fernandez Diaz M., Filip C., Fiorillo G., Franceschi A., Franco D., Frolov E., Funicello N., Gabriele F., Galbiati C., Garbini M., Garcia Abia P., Gendotti A., Ghiano C., Giampaolo R.A., Giganti C., Giorgi M.A., Giovanetti G.K., Gligan M.L., Goicoechea Casanueva V., Gola A., Goretti A.M., Graciani Diaz R., Grigoriev G.Y., Grobov A., Gromov M., Guan M., Guerzoni M., Guetti M., Gulino M., Guo C., Hackett B.R., Hallin A., Haranczyk M., Hill S., Horikawa S., Hubaut F., Hugues T., Hungerford E.V., Ianni A., Ippolito V., James C.C., Jillings C., Kachru P., Kemp A.A., Kendziora C.L., Keppel G., Khomyakov A.V., Kish A., Kochanek I., Kondo K., Korga G., Kubankin A., Kugathasan R., Kuss M., Kuzniak M., La Commara M., La Delfa L., La Grasta D., Lai M., Lami N., Langrock S., Leyton M., Li X., Lidey L., Lippi F., Lissia M., Longo G., Maccioni N., Machulin I.N., Mapelli L., Marasciulli A., Margotti A., Mari S.M., Maricic J., Marinelli M., Martinez M., Martinez Rojas A.D., Martini A., Mascia M., Masetto M., Masoni A., Mazzi A., McDonald A.B., Mclaughlin J., Messina A., Meyers P.D., Miletic T., Milincic R., Miola R., Moggi A., Moharana A., Moioli S., Monroe J., Morisi S., Morrocchi M., Mozhevitina E.N., Mroz T., Muratova V.N., Murenu A., Muscas C., Musenich L., Musico P., Nania R., Napolitano T., Navrer Agasson A., Nessi M., Nikulin I., Nowak J., Oleinik A., Oleynikov V., Pagani L., Pallavicini M., Palmas S., Pandola L., Pantic E., Paoloni E., Paternoster G., Pegoraro P.A., Pellegrini L.A., Pellegrino C., Pelczar K., Perotti F., Pesudo V., Picciau E., Pietropaolo F., Pinna T., Pocar A., Podda P., Poehlmann D.M., Pordes S., Poudel S.S., Pralavorio P., Price D., Raffaelli F., Ragusa F., Ramirez A., Razeti M., Razeto A., Renshaw A.L., Rescia S., Rescigno M., Resnati F., Retiere F., Rignanese L.P., Ripoli C., Rivetti A., Rode J., Romero L., Rossi M., Rubbia A., Rucaj M., Sabiu G.M., Salatino P., Samoylov O., Sanchez Garcia E., Sandford E., Sanfilippo S., Sangiorgio V.A., Santacroce V., Santone D., Santorelli R., Santucci A., Savarese C., Scapparone E., Schlitzer B., Scioli G., Semenov D.A., Shaw B., Shchagin A., Sheshukov A., Simeone M., Skensved P., Skorokhvatov M.D., Smirnov O., Smith B., Sokolov A., Stefanizzi R., Steri A., Stracka S., Strickland V., Stringer M., Sulis S., Suvorov Y., Szelc A.M., Szucs-Balazs J.Z., Tartaglia R., Testera G., Thorpe T.N., Tonazzo A., Torres-Lara S., Tosti S., Tricomi A., Tuveri M., Unzhakov E.V., Usai G., Vallivilayil John T., Vescovi S., Viant T., Viel S., Vishneva A., Vogelaar R.B., Wada M., Wang H., Wang Y., Westerdale S., Wheadon R.J., Williams L., M.Wojcik M., Wojcik M., Xiao X., Yang C., Zani A., Zenobio F., Zichichi A., Zuzel G., Zykova M.P., Centre de Physique des Particules de Marseille (CPPM), Aix Marseille Université (AMU)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physique Nucléaire et de Hautes Énergies (LPNHE (UMR_7585)), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), AstroParticule et Cosmologie (APC (UMR_7164)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Observatoire de Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), DarkSide-20k, Agnes, P., Albergo, S., Albuquerque, I. F. M., Alexander, T., Alici, A., Alton, A. K., Amaudruz, P., Arba, M., Arpaia, P., Arcelli, S., Ave, M., Avetissov, I. C., Avetisov, R. I., Azzolini, O., Back, H. O., Balmforth, Z., Barbarian, V., Barrado Olmedo, A., Barrillon, P., Basco, A., Batignani, G., Bondar, A., Bonivento, W. M., Borisova, E., Bottino, B., Boulay, M. G., Buccino, G., Bussino, S., Busto, J., Buzulutskov, A., Cadeddu, M., Cadoni, M., Caminata, A., Canesi, E. V., Canci, N., Cappello, G., Caravati, M., Cardenas-Montes, M., Cargioli, N., Carlini, M., Carnesecchi, F., Castello, P., Castellani, A., Catalanotti, S., Cataudella, V., Cavalcante, P., Cavuoti, S., Cebrian, S., Cela Ruiz, J. M., Celano, B., Chashin, S., Chepurnov, A., Cicalo, C., Cifarelli, L., Cintas, D., Coccetti, F., Cocco, V., Colocci, M., Conde Vilda, E., Consiglio, L., Copello, S., Corning, J., Covone, G., Czudak, P., D'Aniello, M., D'Auria, S., Da Rocha Rolo, M. D., Dadoun, O., Daniel, M., Davini, S., De Candia, A., De Cecco, S., De Falco, A., De Filippis, G., De Gruttola, D., De Guido, G., De Rosa, G., Della Valle, M., Dellacasa, G., De Pasquale, S., Derbin, A. V., Devoto, A., Di Noto, L., Di Eusanio, F., Dionisi, C., Di Stefano, P., Dolganov, G., Dongiovanni, D., Dordei, F., Downing, M., Erjavec, T., Falciano, S., Farenzena, S., Fernandez Diaz, M., Filip, C., Fiorillo, G., Franceschi, A., Franco, D., Frolov, E., Funicello, N., Gabriele, F., Galbiati, C., Garbini, M., Garcia Abia, P., Gendotti, A., Ghiano, C., Giampaolo, R. A., Giganti, C., Giorgi, M. A., Giovanetti, G. K., Gligan, M. L., Goicoechea Casanueva, V., Gola, A., Goretti, A. M., Graciani Diaz, R., Grigoriev, G. Y., Grobov, A., Gromov, M., Guan, M., Guerzoni, M., Guetti, M., Gulino, M., Guo, C., Hackett, B. R., Hallin, A., Haranczyk, M., Hill, S., Horikawa, S., Hubaut, F., Hugues, T., Hungerford, E. V., Ianni, A., Ippolito, V., James, C. C., Jillings, C., Kachru, P., Kemp, A. A., Kendziora, C. L., Keppel, G., Khomyakov, A. V., Kish, A., Kochanek, I., Kondo, K., Korga, G., Kubankin, A., Kugathasan, R., Kuss, M., Kuzniak, M., La Commara, M., La Delfa, L., La Grasta, D., Lai, M., Lami, N., Langrock, S., Leyton, M., Li, X., Lidey, L., Lippi, F., Lissia, M., Longo, G., Maccioni, N., Machulin, I. N., Mapelli, L., Marasciulli, A., Margotti, A., Mari, S. M., Maricic, J., Marinelli, M., Martinez, M., Martinez Rojas, A. D., Martini, A., Mascia, M., Masetto, M., Masoni, A., Mazzi, A., Mcdonald, A. B., Mclaughlin, J., Messina, A., Meyers, P. D., Miletic, T., Milincic, R., Miola, R., Moggi, A., Moharana, A., Moioli, S., Monroe, J., Morisi, S., Morrocchi, M., Mozhevitina, E. N., Mroz, T., Muratova, V. N., Murenu, A., Muscas, C., Musenich, L., Musico, P., Nania, R., Napolitano, T., Navrer Agasson, A., Nessi, M., Nikulin, I., Nowak, J., Oleinik, A., Oleynikov, V., Pagani, L., Pallavicini, M., Palmas, S., Pandola, L., Pantic, E., Paoloni, E., Paternoster, G., Pegoraro, P. A., Pellegrini, L. A., Pellegrino, C., Pelczar, K., Perotti, F., Pesudo, V., Picciau, E., Pietropaolo, F., Pinna, T., Pocar, A., Podda, P., Poehlmann, D. M., Pordes, S., Poudel, S. S., Pralavorio, P., Price, D., Raffaelli, F., Ragusa, F., Ramirez, A., Razeti, M., Razeto, A., Renshaw, A. L., Rescia, S., Rescigno, M., Resnati, F., Retiere, F., Rignanese, L. P., Ripoli, C., Rivetti, A., Rode, J., Romero, L., Rossi, M., Rubbia, A., Rucaj, M., Sabiu, G. M., Salatino, P., Samoylov, O., Sanchez Garcia, E., Sandford, E., Sanfilippo, S., Sangiorgio, V. A., Santacroce, V., Santone, D., Santorelli, R., Santucci, A., Savarese, C., Scapparone, E., Schlitzer, B., Scioli, G., Semenov, D. A., Shaw, B., Shchagin, A., Sheshukov, A., Simeone, M., Skensved, P., Skorokhvatov, M. D., Smirnov, O., Smith, B., Sokolov, A., Stefanizzi, R., Steri, A., Stracka, S., Strickland, V., Stringer, M., Sulis, S., Suvorov, Y., Szelc, A. M., Szucs-Balazs, J. Z., Tartaglia, R., Testera, G., Thorpe, T. N., Tonazzo, A., Torres-Lara, S., Tosti, S., Tricomi, A., Tuveri, M., Unzhakov, E. V., Usai, G., Vallivilayil John, T., Vescovi, S., Viant, T., Viel, S., Vishneva, A., Vogelaar, R. B., Wada, M., Wang, H., Wang, Y., Westerdale, S., Wheadon, R. J., Williams, L., M. Wojcik, M., Wojcik, M., Xiao, X., Yang, C., Zani, A., Zenobio, F., Zichichi, A., Zuzel, G., Zykova, M. P., Avetissov, I. Ch., Cárdenas-Montes, M., Cicalò, C., D’Aniello, M., D’Auria, S., Ianni, An., Kuźniak, M., Martínez, M., Mróz, T., Sánchez García, E., Wojcik, Ma. M., Wojcik, Ma., Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), and ANR-18-IDEX-0001,Université de Paris,Université de Paris(2018)

Subjects
Astrophysics and Astronomy, Physics - Instrumentation and Detectors, Physics::Instrumentation and Detectors, FOS: Physical sciences, isotopic distillation argon cryogenic, Instrumentation and Detectors (physics.ins-det), dark matter: detector, fabrication, argon: nuclide, astro-ph.IM, dark-matter, DarkSide-20k, Argon, cryogenics, pile-up, [PHYS.PHYS.PHYS-INS-DET]Physics [physics]/Physics [physics]/Instrumentation and Detectors [physics.ins-det], Detectors and Experimental Techniques, Astrophysics - Instrumentation and Methods for Astrophysics, Instrumentation and Methods for Astrophysics (astro-ph.IM), physics.ins-det, performance
Abstract

Aria is a plant hosting a ${350}\,\hbox {m}$ cryogenic isotopic distillation column, the tallest ever built, which is being installed in a mine shaft at Carbosulcis S.p.A., Nuraxi-Figus (SU), Italy. Aria is one of the pillars of the argon dark-matter search experimental program, lead by the Global Argon Dark Matter Collaboration. It was designed to reduce the isotopic abundance of ${^{39}\hbox {Ar}}$ in argon extracted from underground sources, called Underground Argon (UAr), which is used for dark-matter searches. Indeed, ${^{39}\hbox {Ar}}$ is a $\beta $-emitter of cosmogenic origin, whose activity poses background and pile-up concerns in the detectors. In this paper, we discuss the requirements, design, construction, tests, and projected performance of the plant for the isotopic cryogenic distillation of argon. We also present the successful results of the isotopic cryogenic distillation of nitrogen with a prototype plant. The Aria project consists of a plant, hosting a 350 m cryogenic isotopic distillation column, the tallest ever built, which is currently in the installation phase in a mine shaft at Carbosulcis S.p.A., Nuraxi-Figus (SU), Italy. Aria is one of the pillars of the argon dark-matter search experimental program, lead by the Global Argon Dark Matter Collaboration. Aria was designed to reduce the isotopic abundance of $^{39}$Ar, a $\beta$-emitter of cosmogenic origin, whose activity poses background and pile-up concerns in the detectors, in the argon used for the dark-matter searches, the so-called Underground Argon (UAr). In this paper, we discuss the requirements, design, construction, tests, and projected performance of the plant for the isotopic cryogenic distillation of argon. We also present the successful results of isotopic cryogenic distillation of nitrogen with a prototype plant, operating the column at total reflux.

Published
2021
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30. NUDT7 regulates total hepatic CoA levels and the composition of the intestinal bile acid pool in male mice fed a Western diet

Author

Schuyler D. Vickers, Stephanie A. Shumar, Dominique C. Saporito, Amina Kunovac, Quincy A. Hathaway, Breeanna Mintmier, Judy A. King, Rachel D. King, Vazhaikkurichi M. Rajendran, Aniello M. Infante, John M. Hollander, and Roberta Leonardi

Subjects
Cell Biology, Molecular Biology, Biochemistry
Abstract

Nudix hydrolase 7 (NUDT7) is an enzyme that hydrolyzes CoA species, is highly expressed in the liver, and resides in the peroxisomes. Peroxisomes are organelles where the preferential oxidation of dicarboxylic fatty acids occurs and where the hepatic synthesis of the primary bile acids cholic acid and chenodeoxycholic acid is completed. We previously showed that liver-specific overexpression of NUDT7 affects peroxisomal lipid metabolism but does not prevent the increase in total liver CoA levels that occurs during fasting. We generated Nudt7

Published
2022

32. Glyceollin Transcription Factor GmMYB29A2 Regulates Soybean Resistance to Phytophthora sojae

Author

Ryan Percifield, Brianna Harris, Aniello M. Infante, Asraful Jahan, Nik Kovinich, and Matthew Lowery

Subjects
0106 biological sciences, biology, Physiology, Chemistry, food and beverages, Promoter, Plant Science, biology.organism_classification, 01 natural sciences, Elicitor, Cell biology, chemistry.chemical_compound, Glyceollin I, Genetics, Gene silencing, Phytophthora sojae, MYB, Phytophthora, 010606 plant biology & botany, Glyceollin
Abstract

Glyceollin isomers I, II, and III are the major pathogen-elicited secondary metabolites (i.e. phytoalexins) of soybean (Glycine max) that, collectively with other 5-deoxyisoflavonoids, provide race-specific resistance to Phytophthora sojae. The NAC-family transcription factor (TF) GmNAC42-1 is an essential regulator of some but not all glyceollin biosynthesis genes, indicating other essential TF(s) of the glyceollin gene regulatory network remain to be identified. Here, we conducted comparative transcriptomics on soybean hairy roots of the variety Williams 82 and imbibing seeds of Harosoy 63 upon treatment with wall glucan elicitor from P. sojae and identified two homologous R2R3-type MYB TF genes, GmMYB29A1 and GmMYB29A2, up-regulated during the times of peak glyceollin biosynthesis. Overexpression and RNA interference silencing of GmMYB29A2 increased and decreased expression of GmNAC42-1, GmMYB29A1, and glyceollin biosynthesis genes and metabolites, respectively, in response to wall glucan elicitor. By contrast, overexpressing or silencing GmMYB29A1 decreased glyceollin I accumulation with marginal or no effects on the expressions of glyceollin synthesis genes, suggesting a preferential role in promoting glyceollin turnover and/or competing biosynthetic pathways. GmMYB29A2 interacted with the promoters of two glyceollin I biosynthesis genes in vitro and in vivo. Silencing GmMYB29A2 in Williams 82, a soybean variety that encodes the resistance gene Rps1k, rendered it compatible with race 1 P. sojae, whereas overexpressing GmMYB29A2 rendered the susceptible Williams variety incompatible. Compatibility and incompatibility coincided with reduced and enhanced accumulations of glyceollin I but not other 5-deoxyisoflavonoids. Thus, GmMYB29A2 is essential for accumulation of glyceollin I and expression of Phytophthora resistance.

Published
2020
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37. Overexpression of Nudt7 decreases bile acid levels and peroxisomal fatty acid oxidation in the liver

Author

Roberta Leonardi, Stephanie A. Shumar, Evan W. Kerr, Aniello M. Infante, and Paolo Fagone

Subjects
Male, 0301 basic medicine, medicine.drug_class, Coenzyme A, QD415-436, 030204 cardiovascular system & hematology, coenzyme A, Nudix hydrolase, Biochemistry, beta-oxidation, Bile Acids and Salts, Mice, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Endocrinology, Peroxisomes, medicine, Animals, Metabolomics, Pyrophosphatases, Beta oxidation, Research Articles, Triglycerides, chemistry.chemical_classification, Bile acid, Chemistry, Nudix hydrolase 7, Fatty Acids, bile acid metabolism, Lipid metabolism, Cell Biology, Peroxisome, Mice, Inbred C57BL, Metabolic pathway, Cholesterol, 030104 developmental biology, Enzyme, Liver, Oxidation-Reduction, peroxisomes, metabolomics
Abstract

Lipid metabolism requires CoA, an essential cofactor found in multiple subcellular compartments, including the peroxisomes. In the liver, CoA levels are dynamically adjusted between the fed and fasted states. Elevated CoA levels in the fasted state are driven by increased synthesis; however, this also correlates with decreased expression of Nudix hydrolase (Nudt)7, the major CoA-degrading enzyme in the liver. Nudt7 resides in the peroxisomes, and we overexpressed this enzyme in mouse livers to determine its effect on the size and composition of the hepatic CoA pool in the fed and fasted states. Nudt7 overexpression did not change total CoA levels, but decreased the concentration of short-chain acyl-CoAs and choloyl-CoA in fasted livers, when endogenous Nudt7 activity was lowest. The effect on these acyl-CoAs correlated with a significant decrease in the hepatic bile acid content and in the rate of peroxisomal fatty acid oxidation, as estimated by targeted and untargeted metabolomics, combined with the measurement of fatty acid oxidation in intact hepatocytes. Identification of the CoA species and metabolic pathways affected by the overexpression on Nudt7 in vivo supports the conclusion that the nutritionally driven modulation of Nudt7 activity could contribute to the regulation of the peroxisomal CoA pool and peroxisomal lipid metabolism.

Published
2019

38. Retrofit of non-code conforming moment resisting beam-to-column joints: A case study

Author

Tartaglia R., Milone A., D'Aniello M., Landolfo R., Tartaglia, R., Milone, A., D'Aniello, M., and Landolfo, R.

Subjects
Beam-to-column joint, FEM, Seismic retrofitting, Moment resisting frame, Mechanics of Materials, Existing structure, Metals and Alloys, Building and Construction, Civil and Structural Engineering
Abstract

The non-linear monotonic and hysteretic behaviour of as-built and retrofitted beam-to-column joints of an existing non-code conforming steel multi-storey building was investigated by means of finite element simulations. The building case study was originally designed to resist only gravity and wind loads without any capacity design principle. The as-built joints have continuous beam and interrupted columns that are bolted with end-plate splice to the tapered flange of the beams in the nodal zone. These joints were analysed to assess their non-linear response. The finite element analyses showed adequate resistance against gravity loads, but poor performance under seismic and column loss scenarios. Two retrofit strategies were designed in order to enhance the local and global performance in order to guarantee the weak beam-strong column condition, namely: (i) strengthening the column and its connection; (ii) weakening of the beam. Different types of interventions were designed per examined strategy and numerically analysed to assess the global and local response of both external and internal joints. A TOPSIS multicriteria analysis was also performed to identify the most suitable type of intervention.

Published
2022
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39. Separating $${^{39}\hbox {Ar}}$$ <math> <mrow> <msup> <mrow></mrow> <mn>39</mn> </msup> <mtext>Ar</mtext> </mrow> </math> from $${^{40}\hbox {Ar}}$$ <math> <mrow> <msup> <mrow></mrow> <mn>40</mn> </msup> <mtext>Ar</mtext> </mrow> </math> by cryogenic distillation with Aria for dark-matter searches

Author

Agnes, P., Albergo, S., Albuquerque, I., Alexander, T., Alici, A., Alton, A., Amaudruz, P., Arba, M., Arpaia, P., Arcelli, S., Ave, M., Avetissov, I., Avetisov, R., Azzolini, O., Back, H., Balmforth, Z., Barbarian , V., Barrado Olmedo, A., Barrillon, P., Basco, A., Batignani, G., Bondar, A., Bonivento, W., Borisova, E., Bottino, B., Boulay, M., Buccino, G., Bussino, S., Busto, J., Buzulutskov, A., Cadeddu, M., Cadoni, M., Caminata, A., Canesi, E., Canci, N., Cappello, G., Caravati, M., Cárdenas-Montes, M., Cargioli, N., Carlini , M., Carnesecchi, F., Castello, P., Castellani, A., Catalanotti, S., Cataudella, V., Cavalcante, P., Cavuoti, S., Cebrian, S., Cela Ruiz, J., Celano, B., Chashin, S., Chepurnov, A., Cicalò, C., Cifarelli, L., Cintas, D., Coccetti, F., Cocco, V., Colocci, M., Conde Vilda, E., Consiglio, L., Copello, S., Corning, J., Covone, G., Czudak, P., D’Aniello, M., D’Auria, S., Da Rocha Rolo, M., Dadoun, O., Daniel, M., Davini, S., De Candia , A., De Cecco, S., De Falco, A., De Filippis, G., De Gruttola, D., De Guido, G., De Rosa, G., Della Valle, M., Dellacasa, G., De Pasquale, S., Derbin, A., Devoto, A., Di Noto, L., Di Eusanio, F., Dionisi, C., Di Stefano, P., Dolganov, G., Dongiovanni, D., Dordei, F., Downing, M., Erjavec, T., Falciano, S., Farenzena, S., Fernandez Diaz, M., Filip, C., Fiorillo, G., Franceschi, A., Franco, D., Frolov, E., Funicello, N., Gabriele, F., Galbiati, C., Garbini, M., Garcia Abia, P., Gendotti, A., Ghiano, C., Giampaolo, R., Giganti, C., Giorgi, M., Giovanetti, G., Gligan, M., Goicoechea Casanueva, V., Gola, A., Goretti, A., Graciani Diaz, R., Grigoriev, G., Grobov, A., Gromov, M., Guan, M., Guerzoni, M., Guetti, M., Gulino, M., Guo, C., Hackett, B., Hallin, A., Haranczyk, M., Hill, S., Horikawa, S., Hubaut, F., Hugues, T., Hungerford, E., Ianni, An., Ippolito, V., James, C., Jillings, C., Kachru, P., Kemp, A., Kendziora, C., Keppel, G., Khomyakov, A., Kish, A., Kochanek, I., Kondo, K., Korga, G., Kubankin, A., Kugathasan, R., Kuss, M., Kuźniak, M., La Commara, M., La Delfa, L., La Grasta, D., Lai, M., Lami, N., Langrock, S., Leyton, M., Li, X., Lidey, L., Lippi, F., Lissia, M., Longo, G., Maccioni, N., Machulin, I., Mapelli, L., Marasciulli, A., Margotti, A., Mari, S., Maricic, J., Marinelli, M., Martínez, M., Martinez Rojas, A., Martini, A., Mascia, M., Masetto, M., Masoni, A., Mazzi, A., McDonald, A., Mclaughlin, J., Messina, A., Meyers, P., Miletic, T., Milincic, R., Miola, R., Moggi, A., Moharana, A., Moioli, S., Monroe, J., Morisi, S., Morrocchi, M., Mozhevitina, E., Mróz, T., Muratova, V., Murenu, A., Muscas, C., Musenich, L., Musico, P., Nania, R., Napolitano, T., Navrer Agasson, A., Nessi, M., Nikulin, I., Nowak, J., Oleinik, A., Oleynikov, V., Pagani, L., Pallavicini, M., Palmas, S., Pandola, L., Pantic, E., Paoloni, E., Paternoster, G., Pegoraro, P., Pellegrini, L., Pellegrino, C., Pelczar, K., Perotti, F., Pesudo, V., Picciau, E., Pietropaolo, F., Pinna, T., Pocar, A., Podda, P., Poehlmann, D., Pordes, S., Poudel, S., Pralavorio, P., Price, D., Raffaelli, F., Ragusa, F., Ramirez, A., Razeti, M., Razeto, A., Renshaw, A., Rescia, S., Rescigno, M., Resnati, F., Retiere, F., Rignanese , L., Ripoli, C., Rivetti, A., Rode, J., Romero, L., Rossi, M., Rubbia, A., Rucaj, M., Sabiu, G., Salatino, P., Samoylov, O., Sánchez García, E., Sandford, E., Sanfilippo, S., Sangiorgio, V., Santacroce, V., Santone, D., Santorelli, R., Santucci, A., Savarese, C., Scapparone, E., Schlitzer, B., Scioli, G., Semenov, D., Shaw, B., Shchagin, A., Sheshukov, A., Simeone, M., Skensved, P., Skorokhvatov, M., Smirnov, O., Smith, B., Sokolov, A., Stefanizzi, R., Steri, A., Stracka, S., Strickland , V., Stringer, M., Sulis, S., Suvorov, Y., Szelc, A., Szucs-Balazs, J., Tartaglia, R., Testera, G., Thorpe, T., Tonazzo, A., Torres-Lara, S., Tosti, S., Tricomi, A., Tuveri, M., Unzhakov, E., Usai, G., Vallivilayil John, T., Vescovi, S., Viant, T., Viel, S., Vishneva , A., Vogelaar, R., Wada, M., Wang, H., Wang, Y., Westerdale , S., Wheadon, R., Williams, L., M.Wojcik, Ma., Wojcik, Ma., Xiao, X., Yang, C., Zani, A., Zenobio, F., Zichichi, A., Zuzel, G., and Zykova, M.

Abstract

Aria is a plant hosting a $${350}\,\hbox {m}$$ 350 m cryogenic isotopic distillation column, the tallest ever built, which is being installed in a mine shaft at Carbosulcis S.p.A., Nuraxi-Figus (SU), Italy. Aria is one of the pillars of the argon dark-matter search experimental program, lead by the Global Argon Dark Matter Collaboration. It was designed to reduce the isotopic abundance of $${^{39}\hbox {Ar}}$$ 39 Ar in argon extracted from underground sources, called Underground Argon (UAr), which is used for dark-matter searches. Indeed, $${^{39}\hbox {Ar}}$$ 39 Ar is a $$\beta $$ β -emitter of cosmogenic origin, whose activity poses background and pile-up concerns in the detectors. In this paper, we discuss the requirements, design, construction, tests, and projected performance of the plant for the isotopic cryogenic distillation of argon. We also present the successful results of the isotopic cryogenic distillation of nitrogen with a prototype plant.

Published
2021

41. Seismic design rules for ductile eurocode compliant two storey X concentrically braced frames

Author

Costanzo S., D'Aniello M., Landolfo R., Costanzo, S., D'Aniello, M., and Landolfo, R.

Subjects
Capacity design, Two storey CBF, Eurocode 8, Bracing, Concentrically braced frame, Seismic design
Abstract

Two storey X bracings are currently very popular in European practice, as respect to chevron and simple X bracings, owing to the advantages of reducing the bending demand in the brace intercepted beams in V and inverted V configurations and optimizing the design of gusset plate connections. However, rules for two storey X braced frames are not clearly specified within current version of EN1998 1, thus leading to different interpretations of the code by designers. The research presented in this paper is addressed at investigating the seismic behaviour of two storey X concentrically braced frames in order to revise the design rules within EN1998 1. Therefore, five different design criteria are discussed, and their effectiveness is investigated. With this aim, a comprehensive numerical parametric study is carried out considering a set of planar frames extracted from a set of structural archetypes that are representative of regular low, medium and high rise buildings. The obtained results show that the proposed design criteria ensure satisfactory seismic performance.

Published
2020

42. Influence of Transvserse Beams On the Ultimate Behaviour of Seismic Resistant Parrtial Strength Beam-To-Colummn Joints

Author

Tartaglia R., D'Aniello M., Tartaglia, R., and D'Aniello, M.

Subjects
column lo, connection, seismic behaviour, steel joints, steel beam, finite element analysi
Abstract

The most of existing numerical and experimental studies on seismic resistant steel beeam-to-coluumn joints were carried out on planar assemblies, thus disregardding the presence of transvverse beams that typically resist gravity loads in multistorey buildings. In this article, the influence of the transverse beams and their shear connections on the ultimate response of partial strength beam-to-collumn joints is investigated under cyclic actions and column loss scenarios by meeans of finite element simulations. The results show that the presence of the transverse beams provides minor beneficial efeffects on the primary moment resisting joints under seismic actions. On the contraary, the presence of th e transverse beams gives significant effects in the case of collumn loss scenario, where large plastic deforrmations developp following the activation of catenary effects iin the connected members.

Published
2020

43. Separating Ar39 from Ar40 by cryogenic distillation with Aria for dark matter searches

Author

Agnes, P., Albergo, S., Albuquerque, I. F. M., Alexander, T., Alici, A., Alton, A. K., Amaudruz, P., Arba, M., Arpaia, P., Arcelli, S., Ave, M., Avetissov, I. Ch., Avetisov, R. I., Azzolini, O., Back, H. O., Balmforth, Z., Barbarian , V., Barrado Olmedo, A., Barrillon, P., Basco, A., Batignani, G., Bondar, A., Bonivento, W. M., Borisova, E., Bottino, B., Boulay, M. G., Buccino, G., Bussino, S., Busto, J., Buzulutskov, A., Cadeddu, M., Cadoni, M., Caminata, A., Canesi, E. V., Canci, N., Cappello, G., Caravati, M., Cárdenas-Montes, M., Cargioli, N., Carlini , M., Carnesecchi, F., Castello, P., Castellani, A., Catalanotti, S., Cataudella, V., Cavalcante, P., Cavuoti, S., Cebrian, S., Cela Ruiz, J. M., Celano, B., Chashin, S., Chepurnov, A., Cicalò, C., Cifarelli, L., Cintas, D., Coccetti, F., Cocco, V., Colocci, M., Conde Vilda, E., Consiglio, L., Copello, S., Corning, J., Covone, G., Czudak, P., D’Aniello, M., D’Auria, S., Da Rocha Rolo, M. D., Dadoun, O., Daniel, M., Davini, S., De Candia , A., De Cecco, S., De Falco, A., De Filippis, G., De Gruttola, D., De Guido, G., De Rosa, G., Della Valle, M., Dellacasa, G., De Pasquale, S., Derbin, A. V., Devoto, A., Di Noto, L., Di Eusanio, F., Dionisi, C., Di Stefano, P., Dolganov, G., Dongiovanni, D., Dordei, F., Downing, M., Erjavec, T., Falciano, S., Farenzena, S., Fernandez Diaz, M., Filip, C., Fiorillo, G., Franceschi, A., Franco, D., Frolov, E., Funicello, N., Gabriele, F., Galbiati, C., Garbini, M., Garcia Abia, P., Gendotti, A., Ghiano, C., Giampaolo, R. A., Giganti, C., Giorgi, M. A., Giovanetti, G. K., Gligan, M. L., Goicoechea Casanueva, V., Gola, A., Goretti, A. M., Graciani Diaz, R., Grigoriev, G. Y., Grobov, A., Gromov, M., Guan, M., Guerzoni, M., Guetti, M., Gulino, M., Guo, C., Hackett, B. R., Hallin, A., Haranczyk, M., Hill, S., Horikawa, S., Hubaut, F., Hugues, T., Hungerford, E. V., Ianni, An., Ippolito, V., James, C. C., Jillings, C., Kachru, P., Kemp, A. A., Kendziora, C. L., Keppel, G., Khomyakov, A. V., Kish, A., Kochanek, I., Kondo, K., Korga, G., Kubankin, A., Kugathasan, R., Kuss, M., Kuzniak, M., La Commara, M., La Delfa, L., La Grasta, D., Lai, M., Lami, N., Langrock, S., Leyton, M., Li, X., Lidey, L., Lippi, F., Lissia, M., Longo, G., Maccioni, N., Machulin, I. N., Mapelli, L., Marasciulli, A., Margotti, A., Mari, S. M., Maricic, J., Marinelli, M., Martínez, M., Martinez Rojas, A. D., Martini, A., Mascia, M., Masetto, M., Masoni, A., Mazzi, A., McDonald, A. B., Mclaughlin, J., Messina, A., Meyers, P. D., Miletic, T., Milincic, R., Miola, R., Moggi, A., Moharana, A., Moioli, S., Monroe, J., Morisi, S., Morrocchi, M., Mozhevitina, E. N., Mróz, T., Muratova, V. N., Murenu, A., Muscas, C., Musenich, L., Musico, P., Nania, R., Napolitano, T., Navrer Agasson, A., Nessi, M., Nikulin, I., Nowak, J., Oleinik, A., Oleynikov, V., Pagani, L., Pallavicini, M., Palmas, S., Pandola, L., Pantic, E., Paoloni, E., Paternoster, G., Pegoraro, P. A., Pellegrini, L. A., Pellegrino, C., and Pelczar, K.

Subjects
Physics::Instrumentation and Detectors
Abstract

Aria is a plant hosting a 350m cryogenic isotopic distillation column, the tallest ever built, which is being installed in a mine shaft at Carbosulcis S.p.A., Nuraxi-Figus (SU), Italy. Aria is one of the pillars of the argon dark-matter search experimental program, lead by the Global Argon Dark Matter Collaboration. It was designed to reduce the isotopic abundance of 39Ar in argon extracted from underground sources, called Underground Argon (UAr), which is used for dark-matter searches. Indeed, 39Ar is a -emitter of cosmogenic origin, whose activity poses background and pile-up concerns in the detectors. In this paper, we discuss the requirements, design, construction, tests, and projected performance of the plant for the isotopic cryogenic distillation of argon. We also present the successful results of the isotopic cryogenic distillation of nitrogen with a prototype plant.

Published
2021

44. Experimental Stroke Induces Chronic Gut Dysbiosis and Neuroinflammation in Male Mice

Author

Sophia M. Kenney, Wei Wang, Sujung Jun, Heng Hu, Donald A. Primerano, Ryan Percifield, Jennifer Franko, Jessica M Povroznik, Elizabeth B. Engler-Chiurazzi, Sreeparna Chakraborty, Candice M. Brown, Rosana Schafer, James Denvir, Darren E. Gemoets, Divine C. Nwafor, Xuefang Ren, Catheryne A. Gambill, Aniello M. Infante, Maria E. Mace, Allison L. Brichacek, and Stanley A. Benkovic

Subjects
0303 health sciences, medicine.medical_specialty, biology, business.industry, Firmicutes, Sham surgery, Physiology, Gut flora, medicine.disease, biology.organism_classification, 3. Good health, 03 medical and health sciences, 0302 clinical medicine, medicine.anatomical_structure, Medicine, Histopathology, business, Stroke, 030217 neurology & neurosurgery, Feces, Neuroinflammation, 030304 developmental biology, Astrocyte
Abstract

Recent literature implicates gut epithelia mucosa and intestinal microbiota as important players in post-stroke morbidity and mortality. As most studies have focused on the acute effects of stroke on gut dysbiosis, our study objective was to measure chronic, longitudinal changes in the gut microbiota and intestinal pathology following ischemic stroke. We hypothesized that mice with experimental ischemic stroke would exhibit chronic gut dysbiosis and intestinal pathology up to 36 days post-stroke compared to sham controls. Male C57BL/6J mice were subjected to 60 minutes of transient middle cerebral artery occlusion (tMCAO) or sham surgery. To determine the long-term effects of tMCAO on gut dysbiosis, fecal boli were collected pre- and post-tMCAO on days 0, 3, 14, and 28. Bioinformatics analysis demonstrate significant differences in abundance among Firmicutes and Bacteroidetes taxa at the phylum, family, and species levels in tMCAO compared to sham mice that persisted up to one month post-stroke. The most persistent changes in post-stroke microbial abundance were a decrease in bacteria family S24-7 and significant increases inRuminococcaceae. Overall, these changes resulted in a persistently increased Firmicutes:Bacteroidetes ratio in stroke animals. Intestinal histopathology showed evidence of chronic intestinal inflammation that included marked increases in immune cell infiltration with mild-moderate epithelial hyperplasia and villous blunting. Increased astrocyte and microglial activity were also detected one-month post-stroke. These results demonstrate that acute, post-stroke disruption of the gut-brain-microbiota axis progresses to chronic gut dysbiosis, intestinal inflammation, and chronic neuroinflammation.Clinical PerspectivesThe microbiota-gut-brain axis, recently implicated in several neurological disorders, remains largely unexplored at chronic time points post-tMCAO.Our results demonstrate chronic gut dysbiosis, prolonged behavioral deficits, and persistent cerebral and intestinal inflammation post-tMCAO in male C57BL/6J mice.These results suggest that manipulation of microbiota may help reduce poor outcomes after stroke and lead to improved post-stroke functional recovery.

Published
2020
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45. Dysregulation of metabolic‐associated pathways in muscle of breast cancer patients: preclinical evaluation of interleukin‐15 targeting fatigue

Author

Bohlen, Joseph, McLaughlin, Sarah L., Hazard‐Jenkins, Hannah, Infante, Aniello M., Montgomery, Cortney, Davis, Mary, and Pistilli, Emidio E.

Subjects
Cachexia, lcsh:Diseases of the musculoskeletal system, Biopsy, IL‐15, Gene Expression, Breast Neoplasms, Mice, Transgenic, lcsh:QM1-695, Mice, Breast cancer, Biomarkers, Tumor, Animals, Humans, Gene Regulatory Networks, Muscle, Skeletal, Fatigue, Interleukin-15, Optical Imaging, Original Articles, lcsh:Human anatomy, Mitochondria, Disease Models, Animal, Heterografts, Original Article, Female, lcsh:RC925-935, Energy Metabolism, Transcriptome, Metabolic Networks and Pathways
Abstract

Background Breast cancer patients report a perception of increased muscle fatigue, which can persist following surgery and standardized therapies. In a clinical experiment, we tested the hypothesis that pathways regulating skeletal muscle fatigue are down‐regulated in skeletal muscle of breast cancer patients and that different muscle gene expression patterns exist between breast tumour subtypes. In a preclinical study, we tested the hypothesis that mammary tumour growth in mice induces skeletal muscle fatigue and that overexpression of the cytokine interleukin‐15 (IL‐15) can attenuate mammary tumour‐induced muscle fatigue. Methods Early stage non‐metastatic female breast cancer patients (n = 14) and female non‐cancer patients (n = 6) provided a muscle biopsy of the pectoralis major muscle during mastectomy, lumpectomy, or breast reconstruction surgeries. The breast cancer patients were diagnosed with either luminal (ER+/PR+, n = 6), triple positive (ER+/PR+/Her2/neu+, n = 5), or triple negative (ER−/PR−/Her2/neu−, n = 3) breast tumours and were being treated with curative intent either with neoadjuvant chemotherapy followed by surgery or surgery followed by standard post‐operative therapy. Biopsies were used for RNA‐sequencing to compare the skeletal muscle gene expression patterns between breast cancer patients and non‐cancer patients. The C57BL/6 mouse syngeneic mammary tumour cell line, E0771, was used to induce mammary tumours in immunocompetent mice, and isometric muscle contractile properties and fatigue properties were analysed following 4 weeks of tumour growth. Results RNA‐sequencing and subsequent bioinformatics analyses revealed a dysregulation of canonical pathways involved in oxidative phosphorylation, mitochondrial dysfunction, peroxisome proliferator‐activated receptor signalling and activation, and IL‐15 signalling and production. In a preclinical mouse model of breast cancer, the rate of muscle fatigue was greater in mice exposed to mammary tumour growth for 4 weeks, and this greater muscle fatigue was attenuated in transgenic mice that overexpressed the cytokine IL‐15. Conclusions Our data identify novel genes and pathways dysregulated in the muscles of breast cancer patients with early stage non‐metastatic disease, with particularly aberrant expression among genes that would predispose these patients to greater muscle fatigue. Furthermore, we demonstrate that IL‐15 overexpression can attenuate muscle fatigue associated with mammary tumour growth in a preclinical mouse model of breast cancer. Therefore, we propose that skeletal muscle fatigue is an inherent consequence of breast tumour growth, and this greater fatigue can be targeted therapeutically.

Published
2018

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