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Mechanical properties of the topsoil (sandy Podsol and silty Luvisol, FAO) adhering to maize (Zea mays L.) roots and its bulk soil counterpart were studied as a function of soil texture and final soil watersuction at harvest, with three soil water suction values of approximately 30, 50 and 60 kPa. Two scales of observation were also selected: the whole soil:root system and the root-adhering soil aggregates. Three methods were used to characterize the stability of the soil:rootsystem: mechanical shaking in air, and dispersion by low-power ultrasonication, with or without preliminary immersion of the soil:root system in water. Soil disruption kinetics, which were fitted with first-order kinetics equations, were analyzed and discussed. For example, silty soil ultrasonication kinetics, without preliminary water-immersion, could be divided into two parts: the first faster part, which was characterized by a mean rate K value of 6.8-7.2 mJ-1, isattributed to soil slaking, whereas the second slower part, which was characterized by a mean rate K value of 1.5-1.6 mJ-1, was attributed to the rupture of the `firmly root-adhering soil' from the roots. A clear plant effect was observed for both aggregate tensile strength and friability, with higher aggregate strength for the root-adhering silty soil (450-500 kPa) than for its bulk silty soil counterpart (410-420 kPa), and lower friability (coefficient of variationof the aggregate strength) for the root-adhering silty soil (e.g. 67% at a soil water suction value of 30 kPa) than for its bulk silty soil counterpart (e.g. 49% at asoil water suction value of 30 kPa). These effects were attributed to root exudation, which was significantlyhigher for the driest silty topsoil than for the wetter ones. In conclusion, the mechanical properties of the silty topsoil adhering to the maize roots are attributed to both physical and biological interactions occurring in the maize rhizosphere. [ABSTRACT FROM AUTHOR]

This study was designed to investigate the strength of attachment ofplant seedling roots to the soil in which they were grown. The studyalso assessed the effects of differing soil textures and differing soil matric potentials upon the strength of the root:soil attachment. A device for growing roots upon a soil surface was designed, and was used to produce roots which were attached to the soil. In order to quantify root:soil adhesion, roots of maize seedlings, grown on the soil surface, were subsequently peeled off using a universal test machine, in conjunction with simultaneous time-lapse video observation. To clarify the partitioning of energy in the root:soil peeling test, separate mechanical tests on roots, and on two adherent remoulded topsoil balls were also carried out. The seedling root was characterised bya low bending stiffness. The energy stored in bending was negligible, compared to the root:soil adhesion energy. The mechanical properties of two adherent remoulded topsoil balls were a decrease of the soil:soil adhesion energy as the soil:soil plastic energy increased. These two parameters were therefore interdependent. Using a video-camera system, it was possible to separate the different processes occurringduring the root:soil peeling test, in particular, the seed:soil adhesion and the root:soil soil adhesion. An interpretation of the complex and variable force:displacement curves was thus possible, enabling calculation of the root:soil interfacial rupture energy. At a given suction (10 kPa), the results of the peeling test showed a clear soil texture effect on the value of the root:soil interfacial rupture energy. In contrast, for the same silty topsoil, the effect of the soil water suction on the value of the interfacial rupture energy was very moderate. The root:soil interfacial rupture energy was controlled mainly by a product of microscopic soil specific surface area and the macroscopic contact surface area between the root and the soil. Biological [ABSTRACT FROM AUTHOR]

Polychlorinated biphenyls (PCBs) are recognized as persistent organic pollutants and accumulate in organisms, soils, waters, and sediments, causing major health and ecological perturbations. Literature reported PCB bio-transformation by fungi and bacteria in vitro, but data about the in situ impact of those compounds on microbial communities remained scarce while being useful to guide biotransformation assays. The present work investigated for the first time microbial diversity from the three-domains-of-life in a long-term contaminated brownfield (a former factory land). Soil samples were ranked according to their PCB concentrations, and a significant increase in abundance was shown according to increased concentrations. Microbial communities structure showed a segregation from the least to the most PCB-polluted samples. Among the identified microorganisms, Bacteria belonging to Gammaproteobacteria class, as well as Fungi affiliated to Saccharomycetes class or Pleurotaceae family, including some species known to transform some PCBs were abundantly retrieved in the highly polluted soil samples., (© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

Plant growth-promoting rhizobacteria (PGPR) naturally aid plant growth, development and tolerance to stress. Yield increase by the commercial isolate Azospirillum lipoferum CRT1 was recently attributed to an enhanced sprouting success. In order to provide the first biochemical and physiological analysis of sprouting enhancement by PGPR, seed germination and metabolism were followed by time-lapse photography and GC/MS-based metabolomics, respectively, after inoculating two differentially-responding maize cultivars with A. lipoferum CRT1. Bacterial growth on the seeds and plantlet development were also determined. Bacterial inoculation of the seeds of one cultivar led to a 6-8 h hastening of radicle emergence, increased surface bacterial counts, lower contents of energetic primary metabolites before radicle emergence and increased photosynthetic yield, and root surface area, in 3-leaf plantlets. None of these changes were observed on the other maize cultivar that rather accumulated greater levels of stress-related metabolites shortly after radicle emergence. Bacterial counts and cell division-driven central root growth increased in parallel and similarly on both cultivars. A. lipoferum CRT1 stimulated pre-germinating or defense events in a cultivar-dependent manner in maize after rapid (less than 24 h) recognition with initially resting seeds. This PGPR isolate therefore bears agronomic potential as a biopriming agent., (Copyright © 2019 Elsevier GmbH. All rights reserved.)

Plant Growth-Promoting Bacteria (PGPB) of the genus Azospirillum are known to enhance root growth and yield in many plant species including cereals. To probe the underlying mechanisms, correlations between modifications of yield and 6-leaf plantlet characteristics were estimated on maize in four fields with contrasting soil properties over two consecutive years using the commercial isolate A. lipoferum CRT1. In both years, plantlet metabolome, photosynthetic potential and organ morphology were found to display field- and inoculation-specific signatures. Metabolomic analyses revealed that A. lipoferum CRT1 mostly affected sugar metabolism with no suggested impact on N and P assimilation. Mineral nitrogen feeding increased yield but did not affect yield enhancement by the bacterial partner. However, greater improvements of leaf photosynthetic potential correlated with yield diminutions and larger plantlets in all of their proportions correlated with yield enhancements. Bacterial inoculation restored proper seed-to-adult plant ratio when it accidentally dropped below 80%. Only in these cases did it raise yield. All in all, securing mature plant density is hypothesized as being the primary driver of A. lipoferum CRT1-mediated yield enhancement in maize fields.

Eperua falcata (Aublet), a late-successional species in tropical rainforest and one of the most abundant tree in French Guiana, has developed an original strategy concerning N-acquisition by largely preferring nitrate, rather than ammonium (H. Schimann, S. Ponton, S. Hättenschwiler, B. Ferry, R. Lensi, A.M. Domenach, J.C. Roggy, Differing nitrogen use strategies of two tropical rainforest tree species in French Guiana: evidence from (15)N natural abundance and microbial activities, Soil Biol. Biochem. 40 (2008) 487-494). Given the preference of this species for nitrate, we hypothesized that root exudates would promote nitrate availability by (a) enhancing nitrate production by stimulating ammonium oxidation or (b) minimizing nitrate losses by inhibiting denitrification. Root exudates were collected in situ in monospecific planted plots. The phytochemical analysis of these exudates and of several of their corresponding root extracts was achieved using UHPLC/DAD/ESI-QTOF and allowed the identification of diverse secondary metabolites belonging to the flavonoid family. Our results show that (i) the distinct exudation patterns observed are related to distinct root morphologies, and this was associated with a shift in the root flavonoid content, (ii) a root extract representative of the diverse compounds detected in roots showed a significant and selective metabolic inhibition of isolated denitrifiers in vitro, and (iii) in soil plots the abundance of nirK-type denitrifiers was negatively affected in rhizosphere soil compared to bulk. Altogether this led us to formulate hypothesis concerning the ecological role of the identified compounds in relation to N-acquisition strategy of this species., (Copyright © 2013 Elsevier Masson SAS. All rights reserved.)

Inoculation of wheat roots with Paenibacillus (formerly Bacillus) polymyxa CF43 increases the mass of root-adhering soil. We tested the role of levan, a fructosyl polymer produced by strain CF43, in the aggregation of soil adhering to wheat roots. The P. polymyxa gene homologous to the Bacillus subtilis sacB gene encoding levansucrase was cloned and sequenced. The corresponding gene product synthesises high molecular weight levan. A P. polymyxa mutant strain, SB03, whose sacB gene is disrupted, was constructed using heterogramic conjugation. Effects of wheat inoculation with the wild type and the mutant strain were compared using two different cultivated silt loam soils in four independent pot experiments. Roots of wheat plantlets inoculated with CF43 or SB03 were colonized after 7-14 days at the same level, and root and shoot masses were not significantly different from those of the non-inoculated control plants. The ratio of root-adhering soil dry mass to root tissue dry mass was significantly higher for plants inoculated with strain CF43 than for those inoculated with mutant strain SB03: +30% in Orgeval soil and +100% in Dieulouard soil. Thus the levan produced by P. polymyxa is implicated in the aggregation of root-adhering soil on wheat.