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2 results on '"Cynthia Farias Vieira de Melo"'

1. Association study of SNPs of genes IFNGR1 (rs137854905), GSTT1 (rs71748309), and GSTP1 (rs1695) in gastric cancer development in samples of patient in the northern and northeastern Brazil

Author

Eleonidas Moura Lima, Leonardo Ferreira Soares, Rommel Rodríguez Burbano, Juarez Nóbrega da Silva, Arnaldo Correia de Medeiros, Fidelis Manes Neto, Edilson Carvalho Sousa, Cynthia Farias Vieira de Melo, Rubistenia Miranda Soares de Araújo, and Marília de Arruda Cardoso Smith

Subjects
Oncology, medicine.medical_specialty, Population, Single-nucleotide polymorphism, Biology, Bioinformatics, Malignancy, Polymorphism, Single Nucleotide, GSTP1, Stomach Neoplasms, Internal medicine, medicine, Humans, SNP, Genetic Predisposition to Disease, Risk factor, education, Glutathione Transferase, Receptors, Interferon, education.field_of_study, Cancer, General Medicine, medicine.disease, Exact test, Glutathione S-Transferase pi, Brazil
Abstract

Cancer is a multifactorial disease with a high mortality rate in Brazil and worldwide. Gastric cancer (GC) is considered the fourth type of malignancy more frequent in the population worldwide and the second leading cause of death. This work aimed to evaluate single nucleotide polymorphisms (SNPs) of IFNGR1, GSTT1, and GSTP1 genes samples in gastric cancer. We analyzed 60 samples of gastric cancer, 26 diffuse and 34 intestinal types, totaling 120 alleles for each SNP. The results were obtained by PCR and allele-specific PCR. Statistical analyzes performed using BioEstat 5.0 software, applying the Fisher’s exact test and chi-square. Only the SNP gene GSTP1 (rs1695) were significantly associated with gastric cancer in the samples analyzed (χ 2 = 8.73, P

Published
2014
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2. Association of COX2 gene hypomethylation with intestinal type gastric cancer in samples of patients from northern Brazil

Author

Juarez Nóbrega da Silva, Cynthia Farias Vieira de Melo, Marília de Arruda Cardoso Smith, Rubistenia Miranda Soares de Araújo, Carolina Oliveira Gigek, Rommel Rodríguez Burbano, and Eleonidas Moura Lima

Subjects
Male, medicine.medical_specialty, GPX3, Biology, Gastroenterology, Stomach Neoplasms, Internal medicine, RNA, Ribosomal, 16S, Intestinal Neoplasms, medicine, Humans, Promoter Regions, Genetic, Gene, Genetic Association Studies, Glutathione Peroxidase, Helicobacter pylori, Cancer, General Medicine, Methylation, DNA Methylation, biology.organism_classification, medicine.disease, Exact test, Restriction enzyme, Cyclooxygenase 2, DNA methylation, CpG Islands, Female, Brazil
Abstract

To verify the methylation status of THBS1, GPX3, and COX2 genes and to evaluate their association with Helicobacter pylori in gastric adenocarcinomas. Methylation-sensitive restriction enzyme PCR assay was performed in 16 diffuse type gastric cancer samples, 23 intestinal type, and 15 normal stomach tissue. The presence of H. pylori was performed by amplification of the fragment of the 16S rRNA. Statistical analyses were performed using Fisher’s exact test. The hypermethylation of GPX3, THBS1, and COX2 occurred in 18 (n = 7), 5 (n = 2), and 36 % (n = 14) of gastric cancer samples, respectively, whereas in normal samples, it was found in 13, 7, and 67 %. The presence of H. pylori was detected in 67 % of gastric cancer samples and 67 % in normal gastric samples. The methylation of THBS1 and GPX3 was not significantly different between the types of tumors, normal sample, the presence of H. pylori, or clinicopathological variables studied (P > 0.05). However, the methylation status of the gene COX2 is significantly different between normal tissue and intestinal type gastric cancer (P = 0.02). Therefore, our results suggest that the methylation status of the gene COX2 is associated with the intestinal type of gastric cancer.

Published
2013

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