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The AMPK/SNF1 pathway governs energy balance in eukaryotic cells, notably influencing glucose de-repression. In S. cerevisiae, Snf1 is phosphorylated and hence activated upon glucose depletion. This activation is required but is not sufficient for mediating glucose de-repression, indicating further glucose-dependent regulation mechanisms. Employing fluorescence recovery after photobleaching (FRAP) in conjunction with non-linear mixed effects modelling, we explore the spatial dynamics of Snf1 as well as the relationship between Snf1 phosphorylation and its target Mig1 controlled by hexose sugars. Our results suggest that inactivation of Snf1 modulates Mig1 localization and that the kinetic of Snf1 localization to the nucleus is modulated by the presence of non-fermentable carbon sources. Our data offer insight into the true complexity of regulation of this central signaling pathway in orchestrating cellular responses to fluctuating environmental cues. These insights not only expand our understanding of glucose homeostasis but also pave the way for further studies evaluating the importance of Snf1 localization in relation to its phosphorylation state and regulation of downstream targets.

With the more profound use of sustainable developments and degrowth ideas, new trends emerge where urban planners and architects are looking for already built areas that can be re-imaginedas vibrant parts of the city. Most often, those places are post-industrial sites, which are taking a new image and purpose. Multiple examples in European cities shows that those areas are becoming a new vital urban elements and fruitful grounds for implementation of environmentally protective design solutions. However, metamorphic developments of historically important parts of the cities are often susceptible to uninspiring generic design solutions that not necessarily always meet the current needs of citizens. Therefore, this research identifies how such post-industrial zones should be redeveloped with the use of citizens' visions to produce distinctive design solutions that can additionally incorporate sustainable ideas and develop a vibrant area.This research focuses on the post-industrial case in Osijek, Croatia, an old and abandoned Iron Foundry and Machine Factory (OLT). Constructed at the turn of the century (1912.), with its distinguishable architecture, it serves as an important image and industrial heritageof the city. Recently, Osijek Municipality has proposed a plan for its regeneration. Idea became widely appreciated as it was finally decided to somehow protect this valuable public good. However, as this research identifies, the municipal plan does not incorporate any means of citizen participation, nor it goes together with citizens mentioned needs - thus pursuing a plan for developing a generic design. Using qualitative methods, this research is addressing the importance of participation in such projects, as it can focus expertson the hidden meaning and character of the place, derived from citizens’ outputs. Additionally, this research shows that methods suchas participatory workshop, can generate ideas for design solutions that can benefit both current and future u