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Your search keyword '"Cundliffe, Eric"' showing total 216 results
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216 results on '"Cundliffe, Eric"'

6. Type II thioesterase from Streptomyces coelicolor A3(2)

Author

Kotowska, Magdalena, Pawlik, Krzysztof, Butler, Andrew R., Cundliffe, Eric, Takano, Eriko, and Kuczek, Katarzyna

Subjects
Microbiological research -- Analysis, Streptomyces -- Genetic aspects, Esterases -- Genetic aspects, Gene expression -- Physiological aspects, Chromosome mapping -- Genetic aspects, Biological sciences
Abstract

Research has been conducted on the gene encoding a putative type II thioesterase. Results indicate that when expressed in the heterologous host this gene disrupts the native type II thioesterase gene.

Published
2002

7. Feedback control of polyketide metabolism during tylosin production

Author

Butler, Andrew R., Flint, Simon A., and Cundliffe, Eric

Subjects
Polyketides -- Physiological aspects, Enzymes -- Regulation, Feedback control systems -- Physiological aspects, Streptomyces -- Research, Biological sciences
Abstract

Research reveals that biosynthesis of tylosine in Streptomyces fradiae involves polyketide metabolism and synthesis of three sugars. Data indicate that production of mycaminosyl sugar and tylactone stimulate the polyketide metabolism.

Published
2001

9. The mycarose-biosynthetic genes of Streptomyces fradiae, producer of tylosin

Author

Bate, Neil, Butler, Andrew R., Smith, Ian P., and Cundliffe, Eric

Subjects
Macrolide antibiotics -- Physiological aspects, Gene expression -- Analysis, Streptomyces -- Research, Biological sciences
Abstract

Results demonstrate that mycarose-biosynthetic gene(s) tyICVI is part of the polyketide synthase gene complex in Streptomyces fradiae. Sequence and functional aspects of mycarose specific gene product tylosin are presented.

Published
2000

10. Dispensable ribosomal resistance to spiramycin conferred by srmA in the spiramycin producer Streptomyces ambofaciens

Author

Pernodet, Jean-Luc, Gourmelen, Anne, Blondelet-Rouault, Mari-Helene, and Cundliffe, Eric

Subjects
Cytochemistry -- Research, Streptomyces -- Genetic aspects, Ribosomal RNA -- Research, Macrolide antibiotics -- Research, Methylation -- Physiological aspects, Biological sciences
Abstract

The spiramycin producer Streptomyces ambofaciens is discussed relative to the dispensable ribosomal resistance to spiramycin the srmA, a resistance determinant, confers. Several resistance determinants were isolated from S. ambofaciens, one of which was srmA. It hybridized with ermE, the erythromycin-resistance gene from Saccharopolyspora erthraea.

Published
1999

13. Stimulation of polyketide metabolism in Streptomyces fradiae by tylosin and its glycosylated precursors

Author

Fish, Steven A. and Cundliffe, Eric

Subjects
Streptomyces -- Research, Transferases -- Research, Macrolide antibiotics -- Research, Metabolism -- Research, Biological sciences
Abstract

A study was conducted on the stimulation of polyketide metabolism in Streptomyces fradiae. The results show that the targeted disruption of orf2* did not result in the accumulation of tylactone under conditions that normally favor the production of tylosin. Instead, the synthesis of tylactone was eradicated. The findings indicate that glycosylated macrolides have a significantly positive effect on polyketide metabolism in S fradiae.

Published
1997

32. Stimulation of polyketide metabolism in Streptomyces fradiae by tylosin and its glycosylated precursors

Author

Fish, Steven Anthony, Cundliffe, Eric, Fish, Steven Anthony, and Cundliffe, Eric

Abstract

Three glycosyltransferases are involved in tylosin biosynthesis in Streptomyces fradiae. The first sugar to be added to the polyketide aglycone (tylactone) is mycaminose and the gene encoding mycaminosyltransferase is orf2* (tylM2). However, targeted disruption of orf2* did not lead to the accumulation of tylactone under conditions that normally favour tylosin production; instead, the synthesis of tylactone was virtually abolished. This may, in part, have resulted from a polar effect on the expression of genes downstream of orf2*, particularly orf4* (ccr) which encodes crotonyl-CoA reductase, an enzyme that supplies 4-carbon extender units for polyketide metabolism However, that cannot be the entire explanation, since tylosin production was restored at about 10% of the wild-type level when orf2* was re-introduced into the disrupted strain. When glycosylated precursors of tylosin were fed to the disrupted strain, they were converted to tylosin, confirming that two of the three glycosyltransferase activities associated with tylosin biosynthesis were still intact. Interestingly, however, tyladone also accumulated under such conditions and, to a much lesser extent, when tylosin was added to similar fermentations. It is concluded that glycosylated macrolides exert a pronounced positive effect on polyketide metabolism in S. fradiae.

Published
1997

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