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9. A DNA nanostructure‐Hif‐1α inducer complex as novel nanotherapy against cisplatin‐induced acute kidney injury.

Author

Chen, Yuanchong, Xu, Jiangshan, Shi, Sirong, Ma, Wenjuan, Cui, Weitong, Yan, Ran, and Lin, Yunfeng

Subjects
CISPLATIN, ACUTE kidney failure, IRINOTECAN, THERAPEUTICS, NUCLEIC acids, DNA, KIDNEY diseases
Abstract

Since its discovery in 1978, cisplatin‐based chemotherapy regimens have served a pivotal role in human cancer treatment, saving millions of lives. However, its high risk still poses a significant challenge for cisplatin‐induced acute kidney injury (AKI), which occurs in 30% of cisplatin‐treated patients. Unfortunately, no effective solution for preventing or managing this severe complication, which greatly impacts its clinical administration. Kidney is the main organ injured by cisplatin, and the injury is related to cisplatin‐induced cell apoptosis and DNA injury. Therefore, to achieve the safe use of cisplatin in tumour treatment, the key lies in identifying a kidney treatment that can effectively minimize cisplatin nephrotoxicity. Here, we successfully synthesized and applied a DNA‐nanostructure complex, named TFG, which contains tetrahedral framework nucleic acids (tFNAs) and FG‐4592, a novel Hif‐1α inducer. As cargo, TFG is composed entirely of DNA strands. It possesses low nephrotoxicity and renal aggregation properties while FG‐4592 is able to relieve renal injury by downregulating the apoptosis signal pathways. And it can relieve cisplatin‐induced renal injury when taken cisplatin treatment. This work aims to enhance chemotherapy protection in tumour patients by using TFG, a DNA‐based nanomedicines to kidney. This work has the potential to revolutionize the treatment of renal diseases, particularly drug‐induced kidney injury, leading to improved clinical outcomes. [ABSTRACT FROM AUTHOR]

Published
2024
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15. Diverse Routes for the Synthesis of Indole-Fused Complex Architecture from Simple Molecules

Author

Zhang Tianxu, Lalit Ameta Keshav, Saleem Basha M.S., S.C. de Magalhaes Pedro, Zainuddin Mohd., de Souza Aline, Citrome Leslie, I.T. da Silva Julia, C. Carneiro Camila, Singh Shivendra, B. Conforto Adriana, Meng Lingxian, Sun Yue, C. Schulz Victor, Ranjbaran Mehdi, Abbasi Zohreh, Zhao Dan, V. Guesser Vanessa, Todmal Umesh, H.C. de França Paulo, L. Cabral Norberto, N. Silva Vivian, B. Polina Sai, Sheikhganbar Narges, Safanelli Juliana, K. Saini Neeraj, P. Sulochana Suresh, Vakilian Katayon, Sujatha Pothula, Kumar Sharma Neeraj, Cui Weitong, Mullangi Ramesh, Teymouri Farshad, Rezapour Maryam, Kiran Vinay, Diegoli Henrique, B. Gabani Bhavesh, Xiao Dexuan, Lin Yunfeng, C.C. Lopes Cristiane, R. Srinivas Nuggehally, Mardani Mahnaz, Balaji Narayanan, Zhou Ronghui, Singh Chauhan Suresh, Dutt Sunil, B. Liberato Rafaela, Zhang Yuxin, Yasin Noor Mohamed Mohamed, Gao Yang, Li Yanjing, and Amoozegar Hoora

Subjects
Indole test, Chemistry, Simple (abstract algebra), Organic Chemistry, Molecule, Combinatorial chemistry
Abstract

Several easy, efficient and convenient procedures to access indoles fused heterocyclic systems are described in this review. These heterocyclic systems were synthesized from several cyclic or acyclic molecules via a variety of reactions such as Diels-Alder reaction, Michael addition, Morita- Baylis-Hillmann reaction and many other domino/Cascade approaches with high selectivity. Most of the given protocols in this review proceed through organocatalytic pathways; however, some underwent different catalytic and non-catalytic reactions.

Published
2021

18. The Effect of Cognitive Group Counseling on Women’s Motivation for Childbearing-an Interventional Study

Author

Jafari Nodooshan Saeedeh, S.C. de Magalhaes Pedro, Zihlif Malek, Meng Lingxian, Teymouri Farshad, Baqlouq Laila, C. Carneiro Camila, Zainuddin Mohd., Hammad Hana, Diegoli Henrique, N. Silva Vivian, Lin Yunfeng, Sun Yue, Khalafi Leila, Ashrafizadeh Milad, L. Cabral Norberto, Mullangi Ramesh, Rezapour Maryam, V. Guesser Vanessa, Rabie Mahdavi Seyed, I.T. da Silva Julia, Amoozegar Hoora, C.C. Lopes Cristiane, Cui Weitong, Abbasi Zohreh, Vakilian Katayon, R. Srinivas Nuggehally, Kiran Vinay, Mardani Mahnaz, Babu Polina Sai, Balaji Narayanan, M. Abu Thaib Tuqa, Zhou Ronghui, Farhood Bagher, de Souza Aline, Aryafar Tayebeh, Sheikhganbar Narges, P. Sulochana Suresh, Babulal Gabani Bhavesh, Citrome Leslie, Xiao Dexuan, Gao Yang, Eftekhari Seyed-Mohammad, Najafi Masoud, Li Yanjing, Ranjbaran Mehdi, Eleojo Musa Ahmed, Amini Peyman, Zhao Dan, Kumar Saini Neeraj, B. Liberato Rafaela, Zhang Yuxin, C. Schulz Victor, H.C. de França Paulo, Safanelli Juliana, Zhang Tianxu, Todmal Umesh, and B. Conforto Adriana

Subjects
business.industry, Group counseling, Obstetrics and Gynecology, Medicine, Cognition, business, Clinical psychology
Abstract

Background: Postponing parenthood is associated with decreased number of offspring and increased pregnancy risks. Objectives: The present study aimed to investigate the effect of cognitive group counseling on childless couples' decision motivation for childbearing and preconception checkup. Methods: This experimental study was performed on 62 fertile women referred to health care centers in Qom. Signing the written consent form, the participants who were selected by the convenience sampling method entered the study. Childbearing Questionnaire (CBQ) was used to collect data. 6 cognitive group counseling sessions were conducted for the intervention group while the control group did not receive any training except one session on the disadvantages of postponed childbearing by the midwife under the supervision of the researcher. After the intervention, childbearing motivation was evaluated in both groups. The samples were also evaluated in one and two months after the intervention to see whether they have referred for pre-pregnancy checkups. The data were analyzed by SPSS20 using chi-square, and independent t-test. P-value was considered to be less than 0.05. Results: The results showed that after the intervention, the mean of Positive Childbearing Motivation (PCM) in the intervention group was significantly higher than that of the control group and the mean of Negative Childbearing Motivation (NCM) was significantly lower than that of the control group (p Conclusion: Counseling could be more effective to motivate women to start booking for prepregnancy checkups.

Published
2021

25. Increasing the amount of phosphoric acid enhances the suitability of Bradford assay for proteomic research

Author

Qinglu Wang, Jinghua Jin, Cui Weitong, Hongda Cheng, Haibin Zhang, and Huaru Xue

Subjects
Proteomics, Ovalbumin, Detergents, Clinical Biochemistry, 02 engineering and technology, Buffers, 01 natural sciences, Biochemistry, Analytical Chemistry, chemistry.chemical_compound, Chaps, Two dimensional electrophoresis, Lysis buffer, Phosphoric Acids, Phosphoric acid, Bradford protein assay, Chromatography, Chemistry, Chromogenic, 010401 analytical chemistry, Proteins, Globulins, Serum Albumin, Bovine, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Reagent, Yield (chemistry), Biological Assay, Indicators and Reagents, 0210 nano-technology
Abstract

The Bradford assay is one of the most commonly used methods for protein quantification. However, in proteomic research, the lysis buffer generally used for dissolving proteins can cause some interference to the assay. The dye reagent of classical Bradford assay contains 8.50% (w/v) phosphoric acid, which is an important factor relating to the color yield of the assay. In this study, the phosphoric acid content in dye reagent was increased to 9.35% (w/v), 10.20% (w/v), and 11.05% (w/v) to evaluate the changes of interference and the effects of lysis buffer on the interaction between proteins and dye reagent. Results show that lysis buffer not only causes background interference but also affects the protein-dye chromogenic process. Analysis of different components in the lysis buffer showed that carrier ampholyte is the main factor that introduces interference to the Bradford assay. Detergents are well-known interfering compounds in the Bradford assay, but CHAPS and octyl b-D-glucopyranoside only cause slight interference. When the amount of phosphoric acid was increased from 8.50%(w/v) to 10.20% (w/v), the sensitivity of the Bradford assay to proteins in lysis buffer was increased, and the interference delivered by lysis buffer was considerably reduced.

Published
2019

27. Proteomics analysis of pyrene biodegradation using strain Mycobacteriumsp. 16F

Author

Guo, Peng, Jin, Longguo, Cui, Weitong, Yang, Yan, Cheng, Yanjun, Wang, Daoping, Pan, Yinghong, and Jin, Jinghua

Abstract

ABSTRACTMycobacteriumsp. 16 F can degrade 94% of pyrene (20 ppm) in 4 days. To investigate its pyrene degradation mechanism, proteomic changes were analyzed using two-dimensional differential gel electrophoresis (2DE-DIGE). Comparative analysis of differential proteins revealed 91 differentially expressed protein spots after pyrene exposure. Among these, 65 spots were identified as 57 proteins. Further analysis revealed that 13 spots were involved in the pyrene degradation pathway, and most of these were dioxygenases and dehydrogenases. Further, 16 up-regulated expression protein spots were associated with four pathways that may be related to pyrene degradation. Bioinformatics analysis further revealed that the pentose phosphate and glycolytic pathways led to the production of amino acids and nucleotide precursors in pyrene-induced cells. The metabolites from these processes then entered the shikimate pathway via the β-ketoadipate pathway in conjunction with the pyrene degradation pathway. This study provides a new model for the pyrene degradation pathway in Mycobacteria.Abbreviations:PPP, pentose phosphate pathway; ED-EMP, Entner-Doudoroff pathway and Embden-Meyerhof-Parnas pathway; E4P, erythrose-4-phosphate; PEP, phosphoenol-pyruvate; PAL, phenylalanine ammonia-lyase; SucCoA, succinyl-CoA; AcCoA, acetyl-CoA; TCA, tricarboxylic acid cycle.

Published
2022
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43. Neuroprotective and Neurotherapeutic Effects of Tetrahedral Framework Nucleic Acids on Parkinson’s Disease in Vitro

Author

Cui, Weitong, Zhan, Yuxi, Shao, Xiaoru, Fu, Wei, Xiao, Dexuan, Zhu, Junyao, Qin, Xin, Zhang, Tianyi, Zhang, Mei, Zhou, Yi, and Lin, Yunfeng

Abstract

Parkinson’s disease (PD) is a neurodegenerative disease characterized by a series of progressive motor disorders. PD is caused by dysfunction of basal ganglia, decrease of dopaminergic neurons in substantia nigra, and abnormal accumulation of Lewy bodies and Lewy neurites. Antiparkinsonian agents, which are currently used for treatment of PD, exhibit unsatisfactory effects on disease control. In recent years, tetrahedral framework nucleic acids (TFNAs) have been considered as multifunctional nanomaterials, and their scope of application has been extended to a wide range of areas. In previous studies, TFNAs were shown to exert positive effects on various cell types in processes such as cell proliferation, cell differentiation, and apoptosis. In the present study, we explored the role of TFNAs in the treatment and prevention of PD in vitroand elucidated its underlying mechanisms of action. On the basis of the experiments conducted, we demonstrated that TFNAs could inhibit and repair the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced apoptosis of PC12 cells through decreasing the accumulation of α-synuclein, one of the characteristic biomarkers of PD. Genes and proteins related to the AKT/PI3K signaling and mitochondrial apoptotic pathways were examined to further support this finding. Most importantly, TFNAs exhibited unexpected neuroprotective and neurorestorative effects on PC12 cells, providing a novel approach for reducing the neuropathological changes caused by PD.

Published
2024
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44. An Intelligent DNA Nanorobot with in VitroEnhanced Protein Lysosomal Degradation of HER2

Author

Ma, Wenjuan, Zhan, Yuxi, Zhang, Yuxin, Shao, Xiaoru, Xie, Xueping, Mao, Chenchen, Cui, Weitong, Li, Qian, Shi, Jiye, Li, Jiang, Fan, Chunhai, and Lin, Yunfeng

Abstract

DNA nanorobots have emerged as new tools for nanomedicine with the potential to ameliorate the delivery and anticancer efficacy of various drugs. DNA nanostructures have been considered one of the most promising nanocarriers. In the present study, we report a DNA framework-based intelligent DNA nanorobot for selective lysosomal degradation of tumor-specific proteins on cancer cells. We site-specifically anchored an anti-HER2 aptamer (HApt) on a tetrahedral framework nucleic acid (tFNA). This DNA nanorobot (HApt-tFNA) could target HER2-positive breast cancer cells and specifically induce the lysosomal degradation of the membrane protein HER2. An injection of the DNA nanorobot into a mouse model revealed that the presence of tFNA enhanced the stability and prolonged the blood circulation time of HApt, and HApt-tFNA could therefore drive HER2 into lysosomal degradation with a higher efficiency. The formation of the HER2-HApt-tFNA complexes resulted in the HER2-mediated endocytosis and digestion in lysosomes, which effectively reduced the amount of HER2 on the cell surfaces. An increased HER2 digestion through HApt-tFNA further induced cell apoptosis and arrested cell growth. Hence, this novel DNA nanorobot sheds new light on targeted protein degradation for precision breast cancer therapy.

Published
2019
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47. Roles of ERRα and TGF-β signaling in stemness enhancement induced by 1 µM bisphenol A exposure via human neural stem cells.

Author

Dong, Panpan, Ye, Ganghui, Tu, Xinzhuo, Luo, Ying, Cui, Weitong, Ma, Yuxin, Wei, Lei, Tian, Xuewen, and Wang, Qinglu

Subjects
NEURAL stem cells, HUMAN stem cells, AURORA kinases, CELL cycle, CELLULAR signal transduction
Abstract

Bisphenol A (BPA) is a common industrial chemical widely used to produce various plastics and is known to impair neural stem cells (NSCs). However, the effects of low-dose BPA exposure on the stemness maintenance and differentiation fate of NSCs remain unclear in the infant brain. The present study demonstrated that 1 µM BPA promoted human NSC proliferation and stemness, without significantly increasing apoptosis. The Chip-seq experiments demonstrated that both the cell cycle and the TGF-β signaling pathway were accelerated after treatment with 1 µM BPA. Subsequently, estrogen-related receptor α (ERRα) gene knockout cell lines were constructed using CRISPR/Cas9. Further western blotting and chromatin immunoprecipitation-PCR experiments demonstrated that BPA maintained cell stemness by binding to an EERα receptor and activating the TGF-β1 signaling pathway, including the downstream factors Aurora kinases B and Id2. In conclusion, the stemness of NSCs could be maintained by BPA at 1 µM through the activation of the ERRα and TGF-β1 signaling pathways and could restrain the differentiation of NSCs into neurons. The present research further clarified the mechanism of BPA toxicity on NSCs from the novel perspective of ERRα and TGF-β1 signaling pathways regulated by BPA and provided insights into potential novel methods of prevention and therapy for neurogenic diseases. [ABSTRACT FROM AUTHOR]

Published
2022

48. Anti-inflammatory activity of curcumin-loaded tetrahedral framework nucleic acids on acute gouty arthritis.

Author

Zhang M, Zhang X, Tian T, Zhang Q, Wen Y, Zhu J, Xiao D, Cui W, and Lin Y

Abstract

Gouty arthritis is a very familiar inflammatory arthritis. Controlling inflammation is the key to preventing gouty arthritis. However, colchicine, the most highly represented drug used in clinical practice, has strict contraindications owing to some severe side effects. Curcumin (Cur), a natural anti-inflammatory drug, has demonstrated good safety and efficacy. However, the rapid degradation, poor aqueous solubility, and low bioavailability of Cur limit its therapeutic effect. To strengthen the effectiveness and bioavailability of Cur. Cur loaded tetrahedral framework nucleic acids (Cur-TFNAs) were synthesized to deliver Cur. Compared with free Cur, Cur-TFNAs exhibit a preferable drug stability, good biocompatibility (CCK-8 assay), ease of uptake (immunofluorescence), and higher tissue utilization (in vivo biodistribution). Most importantly, Cur-TFNAs present better anti-inflammatory effect than free Cur both in vivo and in vitro experiments through the determination of inflammation-related cytokines expression. Therefore, we believe that Cur-TFNAs have great prospects for the prevention of gout and similar inflammatory diseases., Competing Interests: There is no conflict to declare., (© 2021 The Authors.)

Published
2021
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