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Guangtian Wang,1,2,* Zhihui Li,3,* Gongchen Wang,4,* Qixu Sun,5 Peng Lin,1 Qian Wang,2 Huishu Zhang,6 Yanyan Wang,7 Tongshuai Zhang,8 Feiyun Cui,9 Zhaohua Zhong1,2 1Teaching Center of Pathogenic Biology, School of Basic Medical Sciences, Harbin Medical University, Harbin, Heilongjiang, 150081, People’s Republic of China; 2Department of Microbiology, School of Basic Medical Sciences, Harbin Medical University, Harbin, Heilongjiang, 150081, People’s Republic of China; 3Department of Neurology, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, 150086, People’s Republic of China; 4Department of Vascular Surgery, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, 150086, People’s Republic of China; 5Department of Gastroenterology, Penglai People’s Hospital, Yantai, Shandong, 265600, People’s Republic of China; 6Teaching Center of Biotechnology, School of Basic Medical Sciences, Harbin Medical University, Harbin, Heilongjiang, 150081, People’s Republic of China; 7Teaching Center of Morphology, School of Basic Medical Sciences, Harbin Medical University, Harbin, Heilongjiang, 150081, People’s Republic of China; 8Department of Neurobiology, School of Basic Medical Sciences, Harbin Medical University, Harbin, Heilongjiang, 150081, People’s Republic of China; 9Department of Physiology, School of Basic Medical Sciences, Harbin Medical University, Harbin, Heilongjiang, 150081, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zhaohua Zhong; Feiyun Cui, Email zhongzh@hrbmu.edu.cn; feiyun@hrbmu.edu.cnAbstract: Angiogenesis, or the formation of new blood vessels, is a natural defensive mechanism that aids in the restoration of oxygen and nutrition delivery to injured brain tissue after an ischemic stroke. Angiogenesis, by increasing vessel development, may maintain brain perfusion, enabling neuronal survival, brain plasticity, and neurologic recovery. Induction of angiogenesis and the formation of new vessels aid in neurorepair processes such as neurogenesis and synaptogenesis. Advanced nano drug delivery systems hold promise for treatment stroke by facilitating efficient transportation across the the blood-brain barrier and maintaining optimal drug concentrations. Nanoparticle has recently been shown to greatly boost angiogenesis and decrease vascular permeability, as well as improve neuroplasticity and neurological recovery after ischemic stroke. We describe current breakthroughs in the development of nanoparticle-based treatments for better angiogenesis therapy for ischemic stroke employing polymeric nanoparticles, liposomes, inorganic nanoparticles, and biomimetic nanoparticles in this study. We outline new nanoparticles in detail, review the hurdles and strategies for conveying nanoparticle to lesions, and demonstrate the most recent advances in nanoparticle in angiogenesis for stroke treatment. Keywords: angiogenesis, ischemic stroke, nanoparticles, blood brain barrier, neurogenesis, neuroprotection therapy

Suyang Guo,1 Wenqi Bai,1 Fengjie Cui,1 Xin Chen,1 Xiaojing Fang,1 Honghong Shen,2 Xianhua Gu1 1Department of Gynecology Oncology, First Affiliated Hospital of Bengbu Medical University, Bengbu, People’s Republic of China; 2Department of Medical Oncology, First Affiliated Hospital of Bengbu Medical University, Bengbu, People’s Republic of ChinaCorrespondence: Honghong Shen, Department of Medical Oncology, The First Affiliated Hospital of Bengbu Medical University, Anhui Province Key Laboratory of Translational Cancer Research, Bengbu Medical University, 287 Changhuai Road, Bengbu, Anhui, 233004, People’s Republic of China, Tel +8613385681156, Email shenhonghong1984@163.com Xianhua Gu, Department of Oncology Gynecology, The First Affiliated Hospital of Bengbu Medical University, Bengbu Medical University, 287 Changhuai Road, Bengbu, Anhui, 233004, People’s Republic of China, Tel +8613385680722, Email guxianhua1986@163.comIntroduction: GRHL1 belongs to the family of Grainyhead-like (GRHL). Previous studies have shown that dysregulation of growth and survival pathways is associated with the GRHL family of gene cancers. Immunotherapy with checkpoint inhibitors has changed the treatment paradigm for many tumors, including endometrial cancer (EC). However, the effect of GRHL1 on immunotherapy in EC and its relationship with immune cell infiltration are poorly understood.Methods: Differential expression of GRHL1 between EC and normal EC tissues was analyzed by searching the TCGA database, and the results were verified utilizing immunohistochemistry analyses. Next, the relationship between GRHL1, CD8+ T cells and tumor microenvironment (TME) was also investigated, and the effect of GRHL1 expression on immunotherapy in EC was evaluated.Results: According to the findings, EC tissues had elevated expression levels of GRHL1 relative to normal tissues. Patients with EC who expressed GRHL1 at high levels experienced worse overall survival (OS) and Progression-free survival (PFS) than those whose expression was lower. In addition, GRHL1 expression was negatively correlated with CD8+ T cells, and patients with high GRHL1 expression were less effective in receiving immunotherapy.Conclusion: The expression of GRHL1 was high in EC patients, and high expression of GRHL1 inhibits the proliferation of CD8+ T cells in the tumor microenvironment of EC and affect the efficacy of immunotherapy.Keywords: GRHL1, endometrial cancer, tumor microenvironment, CD8+ T cells, immunotherapy

Yue Wu,1,* Zhiqun Mao,2,* Fengwei Cui,2 Jie Fan,1 Zhouling Yuan,3 Li Tang2 1Department of Geriatric Psychiatry, The Affiliated Mental Health Center of Jiangnan University, Wuxi, Jiangsu, People’s Republic of China; 2Department of Psychiatry, The Affiliated Mental Health Center of Jiangnan University, Wuxi, Jiangsu, People’s Republic of China; 3Department of Psychiatry, Huishan No. 2 People’s Hospital, Wuxi, Jiangsu, People’s Republic of China*These authors contributed equally to this workCorrespondence: Li Tang, Department of Psychiatry, The Affiliated Mental Health Center of Jiangnan University, 156 Qianrong Road, Wuxi, 214151, People’s Republic of China, Tel +86 510 83219318, Fax +86 510 830 12201, Email 839223872@qq.comBackground and Purpose: Subjective cognitive decline (SCD) is recognized as a preclinical indicator of Alzheimer’s disease (AD), and this stage provides a valuable time window for ultra-early intervention in AD. The aim of this study was to investigate the neurocognitive characteristics of SCD and its correlation with objective cognition, negative emotion and sleep quality in Chinese elderly.Methods: A total of 1200 volunteers aged 60 and older underwent Brief Elderly Cognitive Screening Inventory, Quick Cognitive Screening Scale for the Elderly, Generalized Anxiety Disorder-7, Patient Health Questionnaire-9, Pittsburgh Sleep Quality Index and Core Neuropsychological Test. According to the examination results, the participants were divided into healthy control (HC) and SCD groups. The neurocognitive function of SCD and its relationship with objective cognition, negative emotion and sleep quality were analyzed.Results: Compared with the HC group, the SCD group had similar global cognitive function but slightly impaired neurocognitive function. After adjusting for confounding factors such as age, sex, marriage, education and chronic disease, neurocognitive function (Picture-Symbol Matching: OR=0.167, 95% CI: 0.105– 0.266; Word Stem Completion: OR =0.260, 95% CI: 0.131– 0.514; Trail Making Test: OR=0.315, 95% CI: 0.178– 0.560; Picture Recall: OR =0.278, 95% CI: 0.122– 0.636), negative emotion (sub-depressive symptoms: OR=2.287, 95% CI: 1.483– 3.527; sub-anxiety symptoms: OR=1.663, 95% CI: 1.079– 2.563), and poor sleep quality (OR=2.138, 95% CI: 1.571– 2.909) were significantly correlated with the occurrence of SCD.Conclusion: The study illustrates that SCD is closely related to objective cognition, negative emotion and sleep quality. Clinical evaluation and follow-up of SCD should fully account for these factors.Keywords: subjective cognitive decline, cognitive function, negative emotions, sleep quality, Alzheimer′s disease

Guozhong Ma,1 Liuqing Sun,2 Zhiwei Qie,3 Jinzhao He,1 Fang Cui2 1Department of Neurology, Heyuan People’s Hospital, Heyuan, Guangdong Province, 517000, People’s Republic of China; 2Department of Neurology, Hainan Hospital of Chinese People’s Liberation Army General Hospital, Sanya, Hainan Province, 572013, People’s Republic of China; 3Beijing Institute of Radiation Medicine, Beijing, 100850, People’s Republic of ChinaCorrespondence: Guozhong MaDepartment of Neurology, Heyuan People’s Hospital, No. 733 Wenxiang Road, Yuancheng District, Heyuan, Guangdong Province, 517000, People’s Republic of ChinaTel/Fax +86-0762-3185571Email guozhong-ma@hotmail.comFang CuiDepartment of Neurology, Hainan Hospital of Chinese People’s Liberation Army General Hospital, Haitang District, Sanya, Hainan Province, 572013, People’s Republic of ChinaTel/Fax +86- 0898-37330625Email cuifanghaitang@hotmail.comPurpose: To investigate the prevalence of short- and long-term benzodiazepine and z-drugs (BZD) for the treatment of post-stroke subjective sleep disturbance (SSD) and to evaluate the risk factors associated with prolonged BZD treatment in this patient body.Patients and Methods: Between 1st January 2018 and 1st December 2018, we identified 542 inpatients suffering from acute stroke in Heyuan People’s Hospital. Of these, 290 inpatients were included in our final analysis. These patients were divided into three groups according to the treatment they received: non/occasional BZD (non-BZD), short-term BZD (short-term) and prolonged-term BZD (prolonged-term) treatment. We investigated the prevalence of each BZD treatment term and identified differences between the groups. Univariate logistic regression analysis was used to identify potential predictors for the prolonged use of BZD. Multinomial logistic regression analysis was used to assess the correlation between the prolonged use of BZD and potential predictors.Results: The prevalence of cases receiving short and prolonged BZD treatments were 40.35% and 31.72%, respectively; none of the patients received polysomnography (PSG) screening from obstructive sleep apnoea (OSP). Treatment strategies were limited to BZD and traditional Chinese medicine; none of the patients received cognitive-behavioral treatment (CBT) or other forms of treatment. Logistic regression analysis showed that the short-term use was associated with z-drugs (odds ratio [OR]: 2.189, 95% confidence interval [CI]: 1.419– 3.378), non-communication barriers (OR =0.535, 95% CI: 0.325– 0.880) and posterior circulation infarct (POCI) (OR =2.199, 95% CI: 1.112– 4.349). The prolonged-term use was associated with z-drugs (OR =3.012, 95% CI: 1.637– 5.542), non-communication barriers (OR =0.530, 95% CI: 0.307– 0.916), partial anterior circulation infarct (PACI) (OR =0.455, 95% CI: 0.250– 0.827), and non pain after stroke (OR =0.315, 95% CI: 0.207– 0.480).Conclusion: The status of BZD abuse for post-stroke SSD is worrying. Additional research attention and treatment options are needed for the treatment of post-stroke SSD. In particular, the potential combination of stroke and OSP appears to be underestimated and neglected. Post-stroke SSD patients should receive more comprehensive assessment and rigid follow-up to avoid the prolonged use of BZD. Additional and effective therapeutic strategies (such as positive pressure ventilation treatment or CBT) are urgently needed for cause-specific intervention.Keywords: benzodiazepine and z-drugs, BZD, prolonged-use, post-stroke, subjective sleep disturbance, SSD, risk factors, obstructive sleep apnea, OSP, China

Yilun Pei,1,* Fuai Cui,1,2,* Xuejun Du,1 Guowei Shang,1 Wanan Xiao,1 Xinlin Yang,1 Quanjun Cui11Orthopaedic Research Lab, University of Virginia, Charlottesville, VA, USA; 2Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Shandong University, Jinan, Shandong, China*These authors contributed equally to this work Background: There is emerging evidence which suggests that cellular ROS including nitric oxide (NO) are important mediators for inflammation and osteoarthritis (OA). Water-soluble polyhydroxylated fullerene C60 (fullerol) nanoparticle has been demonstrated to have an outstanding ability to scavenge ROS. Purpose: The objective of this study is to assess the effects of fullerol on inflammation and OA by in vitro and in vivo studies.Methods: For in vitro experiments, primary mouse peritoneal macrophages and a macrophage cell line RAW264.7 were stimulated to inflammatory phenotypes by lipopolysaccharide (LPS) in the presence of fullerol. For the animal study, OA model was created by intra-articular injection of monoiodoacetate into the knee joints of rats and fullerol was intravenously injected immediately after OA induction.Results: NO production and pro-inflammatory gene expression induced by LPS was significantly diminished by fullerol in both macrophage cell types. Meanwhile, fullerol could remarkably reduce phosphorylation of p38 mitogen-activated protein kinase, and protein level of transcription factors nuclear factor-kappaB and forkhead box transcription factor 1 within the nucleus. The animal study delineated that systematic administration of fullerol prevented OA, inhibiting inflammation of synovial membranes and the damage toward the cartilage chondrocytes in the OA joints.Conclusion: Antioxidative fullerol may have a potential therapeutic application for OA.Keywords: macrophages, inflammation, osteoarthritis, antioxidant, polyhydroxylated fullerene C60

Shijie Xu,1,* Fangbo Cui,2,3,* Dafu Huang,4,* Dinghu Zhang,5 Anqing Zhu,6 Xia Sun,6 Yiming Cao,7 Sheng Ding,7 Yao Wang,7 Eryun Gao,3 Fenglin Zhang3 1Center for Public Health Research, Medical School, Nanjing University, Nanjing, China; 2Department of Oncology, Wannan Medical College, Wuhu, Anhui, China; 3Department of Oncology, The People’s Hospital of Ma Anshan, Ma Anshan, Anhui, China; 4Department of Oncology, Nanjing Lishui People’s Hospital, Zhongda Hospital Lishui Branch, Southeast University, Nanjing, Jiangsu, China; 5Department of Oncology, Tongde Hospital of Zhejiang Province, Hangzhou, China; 6Department of Oncology, The Affliated Jiangyin Hospital of Southeast University Medical College, Jiangyin, Jiangsu, China; 7Department of General Surgery, The People’s Hospital of Ma Anshan, Ma Anshan, Anhui, China *These authors contributed equally to this work Background: Docetaxel (DOC) is widely used as a chemotherapy drug for various tumors, including gastric cancer (GC), but the clinical application of DOC has been limited due to the hydrophobicity of the drug. We aimed to formulate a multifunctional nanoparticle (NP) system to reduce the side effects of the chemotherapy agent, to promote synergistic therapeutic effects, and to achieve targeted delivery of the therapy. Methods: The polyethylene glycol-poly(ε-caprolactone) NPs (PEG-PCL NPs) were prepared by a ring opening copolymerization technique and were then conjugated with a programmed death-ligand 1 (PD-L1) monoclonal antibody (mAb). The effects of the surface coating on particle size, size distribution, zeta potential, drug encapsulation efficiency, loading capacity, and the drug release kinetics were investigated. By using a panel of PD-L1-expressing human GC cell lines and PD-L1-overexpressing cells, we studied cellular uptake, cytotoxic effects, and cellular apoptosis in the presence of PD-L1 mAb-conjugated NPs. Results: The characterization of the structure and biological functions of DOC-PEG-PCL-mAb NPs was investigated in vitro. X-ray photoelectron spectroscopy validated the presence of the PD-L1 mAbs on the NP surface. The cellular uptake analysis showed that the antibody-conjugated NPs achieved significantly higher cellular uptake. The results of an in vitro cytotoxicity experiment on three GC lines further proved the targeting effects of the antibody conjugation. In addition, we found that the DOC-PEG-PCL-mAb NPs induced cell apoptosis and enhanced G2-M arrest in cancer cells, indicating the inhibition of microtubule synthesis. When compared with the control groups, DOC-PEG-PCL-mAb NPs are more effective in inhibiting PD-L1 expression in GC cells. Conclusion: Our results reported here highlight the biological and clinical potential of DOC-PEG-PCL-mAb NPs using PD-L1 mAbs in GC treatment. Keywords: DOC, gastric carcinoma, PD-L1 monoclonal antibody, nanomedicine, drug delivery

Fang Cui, Wenjia Zhu, Zhibin Zhou, Yuting Ren, Yifan Li, Mao Li, Yunyun Huo, Xusheng Huang Department of Neurology, Chinese PLA General Hospital, Beijing, People’s Republic of China Objectives: To examine the frequency and risk factors of cognitive and anxiety-depressive disorders in patients with amyotrophic lateral sclerosis/motor neuron disease (ALS/MND). Methods: This was an observational study of 100 ALS/MND patients treated at our hospital outpatient and inpatient departments between January 2009 and April 2010 and 100 matched healthy controls. Subjects were surveyed using Mini Mental State Examination (MMSE), Zung Self-Rating Anxiety Scale (SAS), and Zung Self-Rating Depression Scale (SDS). Patient neurological status was graded by the ALS Functional Rating Scale (ALSFRS). Multivariate linear regression was used to identify factors associated with the MMSE, SAS, SDS, and ALSFRS scores. Results: Patients had significantly lower MMSE scores than controls (P0.05). Patients with higher educational level (college and above), shorter disease course (

Shaoping Sun,1 Na Liang,2 Hiromitsu Yamamoto,3 Yoshiaki Kawashima,3 Fude Cui,4 Pengfei Yan1,5 1Key Laboratory of Chemical Engineering Process and Technology for High-efficiency Conversion, College of Heilongjiang Province (Heilongjiang University), School of Chemistry and Material Science, Heilongjiang University, Harbin, People’s Republic of China; 2College of Chemistry and Chemical Engineering, Harbin Normal University, Harbin, People’s Republic of China; 3School of Pharmaceutical Science, Aichi Gakuin University, Nissin, Japan; 4School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, People’s Republic of China; 5Key Laboratory of Functional Inorganic Material Chemistry, Heilongjiang University, Harbin, People’s Republic of China Abstract: This study proposes a new concept of pH-sensitive poly(lactide-co-glycolide) (PLGA) nanoparticle composite microcapsules for oral delivery of insulin. Firstly, insulin–sodium oleate complex was prepared by the hydrophobic ion pairing method and then encapsulated into PLGA nanoparticles by the emulsion solvent diffusion method. In order to reduce the burst release of insulin from PLGA nanoparticles and deliver insulin to specific gastrointestinal regions, hence to enhance bioavailability of insulin, the PLGA nanoparticles were further encapsulated into Eudragit® FS 30D to prepare PLGA nanoparticle composite microcapsules by organic spray-drying method. The preparation was evaluated in vitro and in vivo, and the absorption mechanism was discussed. The in vitro drug release studies revealed that the drug release was pH dependent, and the in vivo results demonstrated that the formulation of PLGA nanoparticle composite microcapsules was an effective candidate for oral insulin delivery. Keywords: sodium oleate, complex, PLGA nanoparticles, Eudragit® FS 30D

Shaoping Sun1–3, Na Liang2, Yoshiaki Kawashima3, Dengning Xia2, Fude Cui21School of Chemistry and Material Science, Heilongjiang University, Harbin, 2School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China; 3School of Pharmaceutical Science, Aichi Gakuin University, Nissin, JapanAbstract: Insulin was complexed with sodium deoxycholate to form an insulin-sodium deoxycholate complex (Ins-SD-Comp) using an hydrophobic ion pairing method in aqueous phase to enhance the liposolubility of insulin. In order to obtain the maximal complexation efficiency, the molar ratio of sodium deoxycholate to insulin was found. The zeta potential method was used to confirm the optimal ratio for formation of Ins-SD-Comp. The structural characteristics of Ins-SD-Comp were assessed using the Fourier transform infrared method. The apparent partition coefficient of insulin increased upon the formation of Ins-SD-Comp. Based on the preliminary study, Ins-SD-Comp was encapsulated into poly(lactide-co-glycolide) (PLGA) nanoparticles using an emulsion solvent diffusion method. The maximal encapsulation efficiency of Ins-SD-Comp into PLGA nanoparticles was 93.6% ± 2.81%, drug loading was about 4.8% ± 0.32%, and the mean diameter of the nanoparticles was 278 ± 13 nm. Biological activity and in vivo results revealed that the bioactivity of insulin was not destroyed during the preparation process. Ins-SD-Comp-loaded PLGA nanoparticles have the potential to reduce serum glucose levels and increase the oral bioavailability of insulin.Keywords: insulin complex, sodium deoxycholate, nanoparticles, zeta potential, oral bioavailability

In practice, little attention has been paid directly to freeze-thaw (FT) cycles during the design and analysis of geogrid-reinforced soil (GRS) walls due to a lack of relevant literature. This study investigates the pavement vertical deformation (s), panel lateral deformation (d), lateral earth pressure (σh), and geogrid strain (ε) of a field GRS wall using an ABAQUS-based numerical model considering variations of the recorded five-year ambient temperature (TR). Numerical results show that the s distribution follows a convex shape instead of the initial concave shape after FT cycles and can be divided into high, transition, and stable deformation zones. FT action alters both location and amplitude of the maximum d within the first two cycles, making the d distribution evolve from a J-shaped curve into an S-shaped one. During freezing, the developments of s and d are coordinated and can be described using a unified model; σh is larger than the Rankine active earth pressure; ε state depends on the interplay of two factors resulting from d and frost heave force. Furthermore, the hysteresis of s, d, σh, and ε with TR was discussed and several beneficial suggestions were proposed for GRS walls to avoid such FT destruction. [ABSTRACT FROM AUTHOR]

Low-temperature synthesis of crystalline silicon and silicon-containing nanowires remains a challenge in synthetic chemistry due to the lack of sufficiently reactive Si precursors. We report that colloidal Si nanowires can be grown using tris(trimethylsilyl)silane or trisilane as the Si precursor by a Ga-mediated solution-liquid-solid (SLS) approach at temperatures of about 200 °C, which is more than 200 °C lower than that reported in the previous literature. We further demonstrate that the new Si chemistry can be adopted to incorporate Si atoms into III-V semiconductor lattices, which holds promise to produce a new Si-containing alloy semiconductor nanowire. This development represents an important step toward low-temperature fabrication of Si nanowire-based devices for broad applications.

This study describes a new species, Sinocyclocheilus xiejiahuai sp. nov., discovered within a cave located in Hongguo Town, Panzhou City, Guizhou Province, southwestern China, with the type locality in the Nanpanjiang River basin. Phylogenetic trees reconstructed based on mitochondrial genes show that the new species represents an independent evolutionary lineage with large genetic differences, 1.9%–13.8% in mitochondrial Cyt b, from congeners. Morphologically, this species can be differentiated from the 79 species currently classified under the genus Sinocyclocheilus by several characteristics: absence of horn-like structures and indistinct elevation at the head-dorsal junction, absence of irregular black markings on the body lateral and scaleless, eyes large, eye diameter 13% of head length, dorsal-fin rays, iii, 6½, last unbranched ray strong, with serrations along posterior margin, pectoral-fin rays, i, 13, anal-fin rays, iii, 5, pelvic-fin rays, i, 7, lateral line pores 74, gill rakers well developed, nine on first gill arch, pectoral fins short, tip not reaching to pelvic-fin origin. The number of Sinocyclocheilus species has been increased from 79 to 80 since the description of this new species.

In order to decode the difference between audiovisual bimodal and unimodal responses of the human brain in semantic context, this study designed a related task paradigm and applied a new generation magnetoencephalogram combined with the machine learning model to analyze the collected signals from three perspectives: behavioral response, event-related field (ERF) and single-trial detection. Results show that the unimodal semantic response was mainly concentrated in the occipital cortex, while the bimodal semantic response was mainly concentrated in the parietal cortex. At the same time, respondents' response rate and the detection accuracy of single-trial in bimodal mode were significantly higher than that in unimodal mode. Moreover, the support vector machine (SVM) showed the best classification performance among the four machine learning models, with an average classification accuracy of 75.16% for within-subject classification and 80.56% for between-subject classification. This research concludes that the combination of optically pumped magnetometer-magnetoencephalography (OPM-MEG) and machine learning model provides an efficient approach to decode the difference between audiovisual bimodal and unimodal responses of the human brain in semantic context.

Objective·To investigate the intervention effect of oral motor training combined with pediatric tui-na on feeding intolerance in preterm infants to provide new treatment options and improve the quality of care.Methods·By using a non-simultaneous controlled clinical trial method, 75 eligible preterm infants from the neonatal unit of a tertiary care hospital in Jinan were selected as the experimental group to implement oral training combined with pediatric massage therapy. Another 60 preterm infants admitted in 2020 were selected as the control group, who had already received oral motor training but had not received pediatric massage. The experimental group was treated for 7 d and the efficacy of the two groups was compared. Comparisons between the two groups included signs of feeding intolerance such as vomiting, gastric remnants and other outcome indicators such as first oral feeding time, gastric tube retention time, increased milk volume at day 3 and increased milk volume at day 7, feeding initiation time, increased weight at day 3 and increased weight at day 7, recovery time of birth weight, and days of hospitalization in both groups.Results·The differences in gender, age, birth weight, and Apgar score between the two groups before treatment were not statistically significant (P>0.05) indicating comparability. After treatment, preterm infants in the experimental group showed significant improvement in feeding performance, first oral feeding time, gastric tube retention time, increased milk volume at day 3 and increased milk volume at day 7, increased weight at day 3 and increased weight at day 7,hospitalization days and other observed indexes compared with the control group, and the differences were statistically significant (P0.05).Conclusion·The combined application of oral motor training and pediatric tui-na is significantly more effective than single oral motor training in the short-term treatment of feeding intolerance in preterm infants. This combination therapy helps preterm infants to increase milk intake and accelerate weight gain, helps the children to recover intestinal function, establishes early nutritional support, promotes growth and development, and reduces the risk of long-term complications. This treatment is simple, safe and efficient, and has the value of popularization.