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Objective To investigate the impact of PD1-TCF1+CD8+ stem-like memory T cells on immunotherapy prognosis and tertiary lymphoid structure in tumors. Methods Pathological tissue sections were collected from 33 patients treated with immunotherapy, 18 cases of NSCLC and 15 cases of ESCC. The expression of PD1-TCF1+CD8+T cells was detected through quantitative analysis by multiplex immunofluorescence. Survival curves were described by the Kaplan-Meier method. Pearson's correlation test was used for correlation analysis. Results The high levels of PD1-TCF1+CD8+T cells had a better PFS in NSCLC and ESCC cohorts. In the NSCLC cohort, high levels of PD1-TCF1+CD8+ T cells were significantly and positively correlated with the number (P=0.0151) and size (P=0.0007) of TLSs. Conclusion In patients with NSCLC and ESCCs, high PD1-TCF1+CD8+ stem-like memory T cell expression indicates improved prognosis and immune response and is associated with the formation of TLSs in the tumor microenvironment of NSCLC.

Mung bean seed was soaked and germinated in CaCl2 electrolyzed water (CEW) in order to investigate the effect and mechanism of CEW on preserving the quality of mung bean sprouts. The results showed that after 5 days of storage, the mass loss percentage of mung bean sprouts treated with electrolyzed CaCl2 solution at a concentration of 25 mmol/L decreased, and the hardness and brittleness increased by approximately 1.28 times higher than compared with the control group treated with tap water. The bactericidal effect of CEW was obvious, reducing the microbial load on the surface of mung bean sprouts by nearly one lg (CFU/g). Low-field nuclear magnetic resonance (NMR) spectroscopy showed that the state of water in mung bean sprouts changed, and the bound water content increased. The relative conductivity, malondialdehyde (MDA) content and hydrogen peroxide content decreased by 16.5%, 27.5%, and 36.8%, respectively, the integrity of the cell membrane was maintained, and the accumulation of reactive oxygen species (ROS) was reduced, thus reducing the degree of membrane lipid peroxidation. In addition, the browning of mung bean sprouts during storage was inhibited by CEW treatment, and the mechanism could be related to the decrease in the content of total phenols as browning substrates and the decrease in the activities of polyphenol oxidase (PPO), peroxidase (POd) and phenylalanine ammonialyase (PAL). Therefore, CEW treatment can delay the quality deterioration of mung bean sprouts during storage to a certain extent.

Objective To explore the effect of peripheral blood markers on the efficacy and prognosis of patients with advanced esophageal cancer treated with immune checkpoint inhibitors (ICIs). Methods The case data of 61 patients with advanced esophageal cancer who met the inclusion criteria were collected. Data on clinical indicators and peripheral blood markers as well as objective response rate (ORR) and progression-free-survival (PFS) were obtained. Results The median PFS of the included patients was 7.10 months (95%CI: 5.12-9.07). The ORR of patients with baseline lactate dehydrogenase (LDH) < 201 was better than that of patients with LDH≥201 (P < 0.05). Univariate analysis showed that baseline LDH0 < 201, neutrophil to lymphocyte ratio (NLR) < 3.9, platelet-to-lymphocyte ratio (PLR) < 240.3, and LDH1 < 249.0 two weeks after ICI treatment were significantly associated with significant improvement in PFS (P < 0.05). In multivariate analysis, patients with NLR0 < 3.9 had longer PFS (P < 0.05). Conclusion LDH0 < 201, NLR0 < 3.9, PLR0 < 240.3, and LDH1 < 249.0 are positively correlated with the prognosis of patients with advanced esophageal cancer treated with ICIs.

In order to improve the security and encryption speed of chaotic encryption system,a lightweight compression image encryption scheme based on chaotic mapping is proposed.Firstly,in order to speed up the encryption process and destroy the correlation between pixels,the digital image is divided into image blocks and compressed by processing in the frequency domain.Then,2D Logistic chaotic mapping is implemented to rearrange and transpose image pixels in the key generation,scrambling and replacement stages.In addition,to further improve the security level,in the diffusion stage,another chaotic map 2D Henon map is used to change the pixel value of the scrambled image.Performance evaluation results show that the proposed scheme can meet the security requirements of image encryption,and the key space is large.Compared with other excellent schemes,this scheme can better resist statistical and differential attacks,and it is highly sensitive to the change of the generated key space.Its key space size is 256bits,the NPCR value is about 99%,and UACI value is more than 15%.

For studying the effects of different addition ways of graphene on the properties of polyaniline/epoxy coatings, the in - situ polymerization method was applied to synthesize polyaniline (PANI)/graphene(G) composite powders on graphene surface, and the as - prepared powders were characterized by X - ray diffractometer, Fourier transform infrared spectrometer and scanning electron microscope. The as - synthesized composite powders, the mixed powders of PANI and G, and the PANI powders were uniformly dispersed in epoxy resin respectively to prepare coatings on the surface of AZ91D magnesium alloy. Subsequently, the effects of different graphene addition methods on the properties of polyaniline/epoxy coatings were studied through electrochemical impedance measurement, water absorption, etc. Results showed that after an immersion within 2 160 h in 3.5% (mass fraction) NaCl solution, the coating added with PANI/G composite powders not only had a higher impedance value in comparision with those added with PANI powders or with PANI/G powders, but also had better flexibility and impact resistance. Compared with the mixed powders of PANI and G, the PANI/G composite powders obtained through in - situ polymerization could better exhibit the shielding effect of G and the corrosion inhibition effect of PANI, and therefore provid better protection to magnesium alloy.