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The analysis of the main germ plasm resources of Morinda officinalis How. in Zhaoqing area and the definition of the relationship among the varieties will provide scientific basis for the cultivation, protection and identification of Morinda officinalis How.. SRAP and CDDP molecular markers were used to analyze the genetic diversity of 9 Morinda officinalis How. materials in Zhaoqing. The genetic similarity coefficients among samples were calculated by NTSYS-pc2.1 software, cluster analysis was carried out according to UPGMA method, and the results of two kinds of molecular markers were compared and analyzed. 28 pairs of SRAP primers and 16 CDDP primers were used to amplify 140 and 83 bands respectively, of which the number of polymorphic bands was 68 and 63 respectively, the polymorphism ratio (PPB) was 48.6% and 75.9% respectively, the genetic similarity coefficient varied from 0.69 to 0.96 and 0.53 to 0.95, and the average genetic similarity coefficient was 0.83 and 0.69 respectively. When the genetic similarity coefficient of SRAP was 0.86 and that of CDDP was 0.71, the four varieties of Guangning special type, Guangning special grafting type, local large leaf type and local lobular type were grouped into one group. SRAP and CDDP molecular markers can be used for genetic diversity analysis of Morinda officinalis How.. There are some differences between SRAP and CDDP, so the combined analysis of the two kinds of markers can more accurately reveal the genetic relationship between Morinda officinalis How. germplasm. The results of CDDP clustering were more consistent with those of combined analysis, indicating that CDDP was more suitable for genetic diversity analysis of Morinda officinalis How. than SRAP.

This paper puts the responsiveness in the view of the tourist of the intangible vulture heritage and research the relation between the responsiveness and the experience of the tourists, It points out that the factor of the responsiveness is the tourists' subjective evaluation of the tourist experience of the intangible culture heritage, defines the conception of responsiveness and the category of the theory research, and constructs the model and extended models of the responsiveness theory. In the theory model of responsiveness, the paper probes two variables which influence tourists' responsiveness of the intangible culture heritage.

Poultry wastes are generally applied to agricultural soils as an organic fertilizer. This final step in the poultry farming management strategy entails a big risk for the environment due to the microorganisms (pathogens) and nutrients contained at high concentrations in these waste materials. The use of biological processes in the field of waste recycling is considered to be a good way to ensure the safety of the obtained products. A new Enterobacteria biological control method, and the possibility of using dictyosteleds to eliminate the undesirable microorganisms in poultry waste were researched in lab condition. Three strains of dictyostelids : Dictyostelium mucoroides 92-12, D. brefeldeum 92-5, and D. minutum 92-7strains were used in the experiment. The results shown: using dictyosteleds to eliminate the undesirable microorganisms in poultry waste is a feasible biological method. Not only the undesirable microorganisms in poultry wastes were eliminated, but also the nutrition ingredient were preserved. After incubated at 20°C for 36h, the quantity of dictyosteleds myxamoeba cells reached to the maximum (around 8.5×107 cell · m L-1); and the quantity of total bacteria, Escherichia, Staphylococcus and Clostridium was 9.8×104 cfu ·m L-1, 4.5×104 cfu ·m L-1, 2.6×104 cfu ·m L-1, 5×101 cfu ·m L-1 respectively. At the end of incubated period (72 h), the quantity of total bacteria, Escherichia, Staphylococcus and Clostridium was 8.0×103 cfu ·m L-1, 8.0 cfu ·m L-1, 5 cfu ·m L-1, 5 cfu ·m L-1, respectively. Comparing the nutrition ingredients between the fresh material and the dictyostelids treated product, There are nearly no decrease of the crude proteins, ash, crude fibre, and crude fat was observed in all dictyostelids trials (p