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Bos indicus cows and their crosses used for milk production are constantly increasing in tropical and subtropical countries, mainly due to their greater adaptability to the climate. However, despite several studies that have reported the greater reactivity of these animals in relation to Bos taurus , there is little information about how Bos indicus cows and their crosses behave in their first lactation and how it affects variables related to production and milking. Thus, we aimed to evaluate the reactivity and milkability of primiparous Holstein, Gyr, and Girolando-F1 (½ Holstein ½ Gyr) cows in the first 21 days of lactation. We used a completely randomized experimental design with repeated measures over time, with 32 cows distributed into three genetic groups. Milk yield, the duration of each milking, and the average and maximum milk flow rates were recorded daily. Variables related to the milking behavior, such as time spent to place the teat cups (min), number of kicks and steps, number of defecation and urination events, and reluctance to be milked score, were evaluated by continuous visual observation at 1, 3, 5, 10, 15, and 20 days in milk (DIM). There was an interaction between genetic groups and days of lactation for milk yield (P < 0.05), where the greatest variations were observed up to the fifth day of lactation, and for time spent for placing the teat cups (P < 0.05), which was 10.2 seconds faster for Holstein cows compared to the other genetic groups at 1 DIM. Also, from 6 to 21 DIM, milking time/kilogram of milk was higher for Gyr cows than for Holstein (P < 0.05), while the differences between Gyr and Girolando-F1 cows varied in the same period. Girolando-F1 cows needed 2.4 more minutes to be milked than Gyr (P < 0.05). The average milk flow rate was higher (P < 0.05) for Holstein cows (1.5 kg/min) compared to Gyr (0.6 kg/min) and Girolando-F1 (0.8 kg/min) cows. The frequency of reluctance score ≥2 was higher (P < 0.05) for Girolando-F1 cows (30.9%) compared with Holstein (8.9%) and Gyr (10%) cows. Stepping increased the probability of Girolando-F1 cows (odds ratio = 1.19; P < 0.01) being classified with a reluctance score ≥2 during milking. Girolando-F1 cows kicked (1.8) and defecated (0.3) more frequently during milking (P < 0.05) compared with Holstein (0.8; 0.0) and Gyr (1.1; 0.1), respectively. In conclusion, reactivity and milkability at the beginning of lactation are distinct for Holstein, Gyr, and Girolando-F1 primiparous cows. Girolando-F1 cows are more reactive at milking, presenting lower milk flow rate and larger milking duration than Holstein cows. • The milking behavior and yield of Holstein, Gyr, and Girolando F1 are different. • Girolando F1 cows were more reactive during milking. • Gyr cows have longer milking times per kilogram of milk and lower maximal milk flow rate. • Holsteins are less reactive and have fastest milk flow and yield more milk. [ABSTRACT FROM AUTHOR]

Background/Objectives: In this study, the differences in protein and fat bioactive components between the milk from purebred Polish Holstein Friesian (PHF) cows and PHF cows crossbred with Swedish Red (SRB) were investigated. The objective was to assess the impact of genetic variation on the nutritional quality of their milk. Methods: This study was conducted at the Warsaw University of Life Sciences' (WULS) experimental dairy farm in Warsaw, Poland, and involved 60 primiparous cows divided into two groups: 30 PHF×SRB crossbred cows and 30 purebred PHF cows. All cows were housed in a free-stall system with an average lactation yield exceeding 10,000 kg/lactation. The milk composition analyses included total protein, casein, whey protein, fatty acid profiles, and vitamin content. Results: Milk from the PHF×SRB hybrids showed a significantly greater total protein content (3.53%) compared to that from the purebred PHF cows (3.28%). The casein content was higher in the hybrids' milk (2.90%) than the purebreds' milk (2.78%), while the whey protein levels were lower in the purebred milk (0.50%) than in the hybrid milk (0.63%). The hybrids exhibited higher concentrations of certain saturated fatty acids in their milk, while the purebreds' milk contained greater amounts of beneficial unsaturated fatty acids and fat-soluble vitamins—E, D, and K. Conclusions: These results indicate that genetic selection through crossbreeding can enhance the nutritional quality of milk. The differences observed in protein, fatty-acid, and vitamin content underscore the role of the genotype in milk composition, suggesting that breeding strategies can optimize dairy products' health benefits. [ABSTRACT FROM AUTHOR]

Dairy cattle production plays a great role in food security and generates income, draught power, and manure input for crop production. The present review focuses on major reproductive performance indicators; age at first service (AFS), age at first calving (AFC), calving interval (CI), days open (DO), and number of services pre conception (NSPC) of improved dairy cattle in Ethiopia. The overall mean values of AFS, AFC, CI, DO, and NSPC were 27.8 ± 5, 37 ± 4.9, 442.7 ± 80.5, 126 ± 40.27 days and 1.57 ± 0.23 numbers, respectively. The review showed the AFS of exotic dairy cattle breeds as 30.5 ± 2.6 for pure exotic, 26.8 ± 3.8 for 75–100%, 25.04 ± 4.5 for 50–75%, 26.7 ± 7 for 50% and 28.1 ± 5.7 days for

This study evaluated heterosis and reciprocal effects in across of White Leghorn with Fayoumi, White Leghorn with Koekoek in comparison to their pure line breeds for the productive and reproductive traits. Data were purposively collected from 357 layers based on their genetic groups and each genotype was reared in three replicates on a deep litter system from 20-72 weeks. Results showed that genotype groups were found to have no significant differences (p > 0.05) for age at first egg (AFE) and hen- housed egg production (HHEP). Purebred Koekoek and crossbred of male White Leghorn and female Koekoek were superior for body weight at first egg (BWAFE). Purebred Fayoumi has exhibited the least values for egg number. Purebred White Leghorn was the best by their feed conversion ratio. Effects of heterosis were non-significant (p > 0.05) for AFE and BWAFE, and hen-housed egg production. A negative heterosis percentage was noted for AFE in all crossbred genotypes. Crossing between male Koekoek and female White Leghorn gave the highest heterosis effect for feed intake while crossing between male White Leghorn and female Fayoumi gave the highest estimates of heterosis for total egg number per hen. The main crossbred between male White Leghorn and female Fayoumi hybrids outperformed in AFE and HHEP while the main crossbred between male White Leghorn and female Koekoek outperformed in BWAFE and HHEP. On the contrary, reciprocal crossbreds between male Fayoumi and female White Leghorn and male Koekoek and female White Leghorn had higher feed intake and better feed conversion ratio.

Abstract Background Essential micronutrient Boron (B) plays crucial roles in plant survival and reproduction but becomes toxic in higher quantities. Although plant cells have different B transport systems, B homeostasis is mainly maintained by two transporter protein families: B exporters (BOR) and nodulin-26-like intrinsic proteins (NIP). Their diversity and differential expression are responsible for varied B tolerance among plant varieties and species. Longan is a highly admired subtropical fruit with a rising market in China and beyond. In the present study, we cultured Shixia (SX) and Yiduo (YD), two differently characterized Longan cultivars, with foliar B spray. We analyzed their leaf physiology, fruit setting, B content, and boron transporter gene expression of various tissue samples. We also traced some of these genes' subcellular localization and overexpression effects. Results YD and SX foliage share similar microstructures, except the mesophyll cell wall thickness is double in YD. The B spray differently influenced their cellular constituents and growth regulators. Gene expression analysis showed reduced BOR genes expression and NIP genes differential spatiotemporal expression. Using green fluorescent protein, two high-expressing NIPs, NIP1 and NIP19, were found to translocate in the transformed tobacco leaves' cell membrane. NIPs transformation of SX pollen was confirmed using magnetic beads and quantified using a fluorescence microscope and polymerase chain reaction. An increased seed-setting rate was observed when YD was pollinated using these pollens. Between the DlNIP1 and DlNIP19 transformed SX pollen, the former germinated better with increasing B concentrations and, compared to naturally pollinated plants, had a better seed-setting rate in YD♀ × SX♂. Conclusion SX and YD Longan have different cell wall structures and react differently to foliar B spray, indicating distinct B tolerance and management. Two B transporter NIP genes were traced to localize in the plasma membrane. However, under high B concentrations, their differential expression resulted in differences in Jasmonic acid content, leading to differences in germination rate. Pollination of YD using these NIPs transformed SX pollen also showed NIP1 overexpression might overcome the unilateral cross incompatibility between YD♀ × SX♂ and can be used to increase Longan production.

The quality of pork meat directly influences the price and consumption. The genetic improvement of pigs has mainly focused on high productive efficiency, which has resulted in poor meat quality. Crossbreeds containing commercial and indigenous breeds could improve the meat quality, but identifying breed composition was difficult because of the lack of an ancestry reference panel. Therefore, we first constructed an abundant reference panel and convenient pipeline to identify ancestry/breed composition. The ancestry reference panel consisted of 517 reliable individuals, including three commercial breeds (Duroc, Landrace, and Yorkshire) and 38 indigenous Chinese breeds. The nature of the reference panel showed that the European domestic breed (EUD) and Asian domestic breed (ASD) were distinctly divided into two clusters. The evaluation of ancestry identification revealed that the reference panel performed well in identifying EUD and ASD ancestry proportions for commercial breeds, indigenous breeds, and crossbreeds. In addition, the ancestry reference panel also performed excellently in identifying breed composition for 3 commercial and 38 indigenous breeds. Specifically, the reference panel showed the outstanding identification of breed composition for crossbred individuals. These results suggested that the ancestry reference panel and convenient pipeline played a good role in identifying breed composition for pigs.

This study evaluated heterosis and reciprocal effects in across of White Leghorn with Fayoumi, White Leghorn with Koekoek in comparison to their pure line breeds for the productive and reproductive traits. Data were purposively collected from 357 layers based on their genetic groups and each genotype was reared in three replicates on a deep litter system from 20-72 weeks. Results showed that genotype groups were found to have no significant differences (p > 0.05) for age at first egg (AFE) and hen-housed egg production (HHEP). Purebred Koekoek and crossbred of male White Leghorn and female Koekoek were superior for body weight at first egg (BWAFE). Purebred Fayoumi has exhibited the least values for egg number. Purebred White Leghorn was the best by their feed conversion ratio. Effects of heterosis were nonsignificant (p > 0.05) for AFE and BWAFE, and hen-housed egg production. A negative heterosis percentage was noted for AFE in all crossbred genotypes. Crossing between male Koekoek and female White Leghorn gave the highest heterosis effect for feed intake while crossing between male White Leghorn and female Fayoumi gave the highest estimates of heterosis for total egg number per hen. The main crossbred between male White Leghorn and female Fayoumi hybrids outperformed in AFE and HHEP while the main crossbred between male White Leghorn and female Koekoek outperformed in BWAFE and HHEP. On the contrary, reciprocal crossbreds between male Fayoumi and female White Leghorn and male Koekoek and female White Leghorn had higher feed intake and better feed conversion ratio. [ABSTRACT FROM AUTHOR]

The aim of this review was to present the breeding status of pure or crossbreeds of European cattle with African indigenous cattle in order to improve milk yield in African tropical conditions. As the African indigenous breeds are characterized by small size, low body weight (300–450 kg) and low production performances (less than 1000 kg per lactation), short lactation length (205–240 days), etc., the crossbreeding with pure European cattle was done in order to ameliorate production performances especially milk and beef production. As results, it was seen that F1 offspring improved output productions than their parents of tropical origin. Also, it was seen that F1 offspring showed disease and heat resistance than their parents of temperate climate origin. However, at different degrees of crossbreeding, it was seen that the offspring can demonstrate bad production performances than their parents and therefore, the crossbreeding has to respect a certain degree. Other consequences are that the crossbreeding of local and pure European cattle breeds has resulted in calving complications in African local cattle. In African indigenous cattle, the selection as an improvement tool was given less emphasis as a solution to improve output production. That is why, the implementation of a well-prepared selection programs could be practiced in African indigenous cattle in order to preserve African cattle genotypes, as they are endangered of extinction due to various crossbreeding with exotic cattle breeds. [ABSTRACT FROM AUTHOR]

Abstract. The aim of the study was to assess survival rate of crossbred New Zealand White and Dutch rabbit breed. A total of 16 mature rabbits of seven to nine months old, New Zealand White and Dutch were used for the study. Data obtained were subjected to analysis of variance ANOVA using SAS. The reproductive traits studied were litter size, litter birth weight, mean kit weight. The growth traits were: body weight, body length, ear length, chest circumference, hind limbs, thigh length, nose to shoulder and thigh circumference. The reproductive traits were significantly (P

Iberian ham is a highly appreciated product and according to Spanish legislation different labels identify different products depending on the genetic purity. Consequently, “100% Iberian” ham from purebred Iberian animals is more expensive than “Iberian” ham from Iberian x Duroc crosses. The hypothesis of this study was that to avoid labelling fraud it is possible to distinguish the breed (Iberian or Iberian x Duroc) of acorn-fed pigs of Iberian ham without any prior preparation of the sample by using spectroscopy that is a rapid and reliable technology. Moreover, portable devices which can be used in situ could provide similar results to those of benchtop equipment. Therefore, the spectra of the 60 samples (24 samples of 100% Iberian ham and 36 samples of Iberian x Duroc crossbreed ham) were recorded only for the fat, only for the muscle, or for the whole slice with two benchtop near-infrared (NIR) spectrometers (Büchi NIRFlex N-500 and Foss NIRSystem 5000) and five portable spectrometers including four portable NIR devices (VIAVI MicroNIR 1700 ES, TellSpec Enterprise Sensor, Thermo Fischer Scientific microPHAZIR, and Consumer Physics SCiO Sensor), and one RAMAN device (BRAVO handheld). The results showed that, in general, the whole slice recording produced the best results for classification purposes. The SCiO device showed the highest percentages of correctly classified samples (97% in calibration and 92% in validation) followed by TellSpec (100% and 81%). The SCiO sensor also showed the highest percentages of success when the analyses were performed only on lean meat (97% in calibration and 83% in validation) followed by microPHAZIR (84% and 81%), while in the case of the fat tissue. Raman technology showed the best discrimination capacity (96% and 78%) followed by microPHAZIR (89% and 81%). Therefore, spectroscopy has proved to be a suitable technology for discriminating ham samples according to breed purity; portable devices have been shown to give even better results than benchtop spectrometers.

The aim of the study was to assess survival rate of crossbred New Zealand White and Dutch rabbit breed. A total of 16 mature rabbits of seven to nine months old, New Zealand White and Dutch were used for the study. Data obtained were subjected to analysis of variance ANOVA using SAS. The reproductive traits studied were litter size, litter birth weight, mean kit weight. The growth traits were: body weight, body length, ear length, chest circumference, hind limbs, thigh length, nose to shoulder and thigh circumference. The reproductive traits were significantly (P<0.05) affected by genetic group Pure and crosses. New Zealand White observed high litter size and litter birth weight at first, seventh, 14th and 21st day. Purebred Dutch had significant (P<0.05) high values for growth traits. The body weight 478.73±18.10, body length 33.12±0.42, ear length 9.10±0.09, chest circumference 18.24±0.45 and hind limbs 19.51±0.33 were significantly (P<0.05) high in crosses of Dutch x New Zealand White. Pre-weaning mortality and survival rate at weaning were 22.22% and 77.77% high in New Zealand White; 14.28% and 85.71% in Dutch and DUC x NZW; and 9.09% and 90.90% in NZW x DUC. In conclusion, New Zealand White x Dutch crosses demonstrated high survival rate and low pre-weaning mortality. [ABSTRACT FROM AUTHOR]

Kacang (K) and Kacang Etawah (KE) cross goats are the major goat breeds that are important for farming livelihood and income and recognized for their tolerance to hot and humid tropical climates. As global warming progresses, the daily maximum temperature (Tmax) is predicted to be continuously increased, which will challenge goat production in the future. The objective of this study was to evaluate the physiological and behavioral responses of the goats to the elevated Tmax. Six K and six KE female goats were housed in a normal environment (average Tmax: 33°C; temperature humidity index (THI): 76 to 86) for 6 weeks and then in a hot environment (average Tmax: 38°C; THI: 76 to 92) for 7 weeks. During hot conditions, rectal, rectal surface, and skin temperature, respiration rate, hemoglobin, and cholesterol increased (p < 0.05), whereas glucose blood levels decreased (p < 0.01). Dry matter (DM) intake was lowered (p < 0.01), and DM digestibility was elevated (p < 0.01), whereas drinking water and body water retention were lowered but varied (p < 0.05) during hot weeks. Lying time increased during the hot weeks in both breeds (p < 0.01), whereas lying and ruminating as well as total ruminating time was longer (p < 0.05) in KE goats compared to K goats, which explain the greater (p < 0.05) DM digestibility in KE goats. The effect of the elevated Tmax might be less severe since it also depends on the duration of the Tmax and the variation of daily THI. [ABSTRACT FROM AUTHOR]

This study was carried out to compare some production traits of Alpine × Hair-crossbred, Saanen × Haircrossbred, and Hair goats in rural conditions. In the first year of the study, a total of 45 animals were used to determine the milk yield characteristics of the three genotypes. In the second year, a total of 56 does and 62 goat kids were used to determine the characteristics of the animals. The ICAR AT method was used to calculate the lactation milk yield of the goats. The average lactation lengths, the average lactation milk yields, and the daily average milk yields of Alpine × Hair-crossbred, Saanen × Hair-crossbred, and Hair goats were 242, 232, and 229 days; 182, 201, and 164 kg; and 0.754, 0.873, and 0.716 kg, respectively. The effect of genotype on lactation milk yield and daily average milk yield was statistically significant. Live weights of the kids were recorded at monthly intervals and live weights of the kids for days 30, 60, 90, and 120 were calculated by linear interpolation. The effect of genotype on the 90- and 120-day weights were similar. Measurements of udder characteristics were determined twice, at the beginning (30 d) and at the end (180 d) of the lactation period. It was determined that crossbreeding had no effect on udder characteristics except the distance between teats and udder depth. The main result of this study is that crossbreeding substantially increased milk yield in goats, even at the F1 level, under extensive conditions. [ABSTRACT FROM AUTHOR]

The article discusses research which described the development and evaluated cross breed hybrids for Bombyx mori bidensovirus (BmBDV) resistance through molecular marker assisted selection. The study details the screening and selection of silkworm breeds for BmBDV resistance, isolation of genomic DNA, BmBDV resistant marker analysis and BmBDV inoculum preparation. It identifies the economic traits of BmBDV resistant cross breeds, mortality rate and survival rate for BmBDV infection.

This study was conducted to examine the milk yield traits of Holstein Friesian (HF), Brown Swiss (BS), and Simmental (SM) breeds, as well as their crossbreeds raised in enterprises registered in the herdbook system in Türkiye. With this study, the performances of cattle breeds and their crossbreeds widely raised in Türkiye were compared in terms of milk yield characteristics based on agricultural regions of Türkiye. The data used in the study included the completed lactation records of 22 331 heads of cattle for the years 2000-2014. The GLM ANOVA method was used in the statistical analysis of the effects of breed, genotype, and region on traits related to milk yield. Across the herd, the mean and standard deviation values were determined as 7551±2771 kg for lactation actual milk yield (AMY), 4.2±1.8 for total lactation number (TLN), and 382.9±9.2 days for lactation period (LP). The effects of genotypes and regions were found to be significant or very significant (p<0.05 or p<0.001) for all three studied traits, region x genotype interaction was found to be very significant for AMY and TLN (p<0.001), and not significant for LP (p=0.184). As a result, it was revealed that the interaction effects of genotype, region, and genotype x region should be taken into account in determining the milk yield traits of cultured breed and crossbreed cows that are widely raised in Türkiye. Accordingly, it was concluded that the interaction findings should be evaluated in determining the breed preferences by the regions. [ABSTRACT FROM AUTHOR]

The congenital disability, atresia ani or atresia ani et recti or recto-vaginal fistula, is one of the most common types of congenital malformation disability. A 45-day-old male cross-breed calf was admitted to Patuakhali Science and Technology University's Veterinary Teaching Hospital with complaints of leaking feces through the umbilicus and was clinically confirmed as a recto-umbilicus fistula. This congenital condition was corrected successfully by making an artificial opening in the lower right abdominal wall. Corrective surgery was the only successful technique for repairing the recto-umbilicus fistula. The prognosis for surgical correction of the recto-umbilicus fistula in a cross-breed calf was indicated to be good.

Conception Rate (CR) and Service Conception (S/C) are two parameters for assessing the reproductive performance of cattle. This research was conducted to determine the value of S/C and CR in crossbreeding cattle in the working area of the Public Health Center. The material used in this study was 72 crossbreed cattle aged 3-10 years. Semi-intensive rearing system with AI mating. The results the S/C in this study was 3.9 higher than the ideal standard of 1.6–2. The CR in this study was 58.6%. CR numbers are still relatively low from the ideal, namely 65 -75%. [ABSTRACT FROM AUTHOR]

Brangus is a composite cattle breed developed with the objective of combining the advantages of Angus and Zebuine breeds (Brahman, mainly) in tropical climates. The aim of this work was to estimate breed composition both genome‐wide and locally, at the chromosome level, and to uncover genomic regions evidencing positive selection in the Argentinean Brangus population/nucleus. To do so, we analysed marker data from 478 animals, including Brangus, Angus and Brahman. Average breed composition was 35.0% ± 9.6% of Brahman, lower than expected according to the theoretical fractions deduced by the usual cross‐breeding practice in this breed. Local ancestry analysis evidenced that breed composition varies between chromosomes, ranging from 19.6% for BTA26 to 56.1% for BTA5. Using approaches based on allelic frequencies and linkage disequilibrium, genomic regions with putative selection signatures were identified in several chromosomes (BTA1, BTA5, BTA6 and BTA14). These regions harbour genes involved in horn development, growth, lipid metabolism, reproduction and immune response. We argue that the overlapping of a chromosome segment originated in one of the parental breeds and over‐represented in the sample with the location of a signature of selection constitutes evidence of a selection process that has occurred in the breed since its take off in the 1950s. In this regard, our results could contribute to the understanding of the genetic mechanisms involved in cross‐bred cattle adaptation and productivity in tropical environments. [ABSTRACT FROM AUTHOR]

This study evaluated (i) whether weight gain and levels of inflammatory and immune markers including white blood cells (WBC), serum haptoglobin, C-reactive protein, albumin, and globulin fractions change between the Nero Siciliano pig breed and the crossbreed Landrace x Large White (LxLW) reared under the same environmental and farming conditions; and (ii) whether a liquid whey diet supplementation affects the investigated parameters in both genotypes. In this study, 10 crossbreed LxLW and 10 Nero Siciliano pigs were given control feed, representing the control groups (CTRC and CTRNS), whereas 10 crossbreed LxLW and 10 Nero Siciliano pigs were given control feed supplemented with liquid whey for 2 months, representing the experimental groups (WC and WNS). From each pig, body weight and blood were collected before experimental diet supplementation (T0), and one (T1) and two (T2) months after the start of the diet supplemented with whey. The white blood cell count (WBC), serum haptoglobin, C-reactive protein, total proteins, albumin and globulin fraction concentration were assessed. Two-way analysis of variance showed an increasing trend of body weight both in the control and experimental groups of the two pig genotypes throughout the monitoring period (p < 0.01) without a significant effect of genotype and diet (p > 0.05). The concentration of haptoglobin, β1- and β2-globulins was affected by pig genotype, diet supplementation, and time (p < 0.01). The values of WBC, C-reactive protein, albumin, α-globulins, and A/G ratio were affected by diet supplementation (p < 0.01) and time (p < 0.01) without an influence of genotype (p > 0.05). Nero Siciliano pigs showed lower levels of haptoglobin, β1-globulin, and β2-globulin compared to crossbreed LxLW. Nero Siciliano pigs and crossbred LxLW fed with liquid whey showed lower levels of WBC, haptoglobin, C-reactive protein, α-, β1-, and β2-globulins and higher values of albumin compared to control groups. The results reinforced the hypothesis that autochthonous breeds possess higher resilience to farming conditions when compared to allochthonous breeds. Moreover, an immune-modulatory and an anti-inflammatory power of liquid whey dietary supplementation is suggested probably thanks to its content in natural bioactive substances including anti-inflammatory cytokines and anti-oxidative factors.

Crossbreeding is a process in which animals from different breeds are mated together. The animals produced will exhibit a combination of both additive and non-additive genetic improvement from parental breeds that increase heterozygosity and negate inbreeding depression. However, crossbreeding may also break up the unique and often beneficial gene combinations in parental breeds, possibly reducing performance potential as the benefits of heterosis depends on the type of crossbreeding systems used and heritability of the traits. This effect of crossbreeding, especially on the genome architecture, is still poorly understood with respect to 3-breed crossbreeding systems. Thus, this study examined variation in genomic ancestry estimations relative to pedigree-based estimations and correlated breed composition to key production and health traits. Two rotational crossbred populations, referenced as ProCROSS and Grazecross were assessed and totaled 607 crossbred cattle. ProCROSS is a product of rotational crossbreeding of Viking Red (VKR), Holstein (HOL), and Montbeliarde (MON). In contrast, Grazecross consists of Viking Red (VKR), Normande (NOR), and Jersey (JER). Both breeding programs were aimed at capitalizing on the positive effect of heterosis. The VKR is a marketing term for Swedish Red, Danish Red, and Finnish Ayrshire breed which complicated breed determination. Therefore, genomic breed composition estimates were compared using two different representations of VKR, one of which was based on parents used in the crossing system and a second based on genotypes from the ancestral breeds that comprise VKR. Variation of breed composition estimates were assessed between pedigree and genome-based predictions. Lastly, Genomic estimations were correlated with production and health traits by comparing extreme performance groups to identify the relationship between breed ancestry and performance. With the exception of the JER breed composition in Grazecross, all other estimates of the purebred contribution to the ProCROSS and Grazecross showed a significant difference in their genomic breed estimation when using the VKR ancestral versus the VKR parental reference populations for admixture analysis. These observations were expected given the different relationship of each VKR representation to the crossbred cattle. Further analysis showed that regardless of which VKR reference population was used, the degree of MON and HOL breed composition plays a significant role in milk and fat production in ProCROSS, while the degree of VKR and NOR ancestry were related to improved health performance in Grazecross. In all, identifying the most appropriate and informative animals to use as reference animals in admixture analysis is an important factor when interpreting results of relationship and population structure, but some degree of uncertainty exists when assessing the relationship of breed composition to phenotypic performance. [ABSTRACT FROM AUTHOR]

Crossbreeding is a process in which animals from different breeds are mated together. The animals produced will exhibit a combination of both additive and non-additive genetic improvement from parental breeds that increase heterozygosity and negate inbreeding depression. However, crossbreeding may also break up the unique and often beneficial gene combinations in parental breeds, possibly reducing performance potential as the benefits of heterosis depends on the type of crossbreeding systems used and heritability of the traits. This effect of crossbreeding, especially on the genome architecture, is still poorly understood with respect to 3-breed crossbreeding systems. Thus, this study examined variation in genomic ancestry estimations relative to pedigree-based estimations and correlated breed composition to key production and health traits. Two rotational crossbred populations, referenced as ProCROSS and Grazecross were assessed and totaled 607 crossbred cattle. ProCROSS is a product of rotational crossbreeding of Viking Red (VKR), Holstein (HOL), and Montbeliarde (MON). In contrast, Grazecross consists of Viking Red (VKR), Normande (NOR), and Jersey (JER). Both breeding programs were aimed at capitalizing on the positive effect of heterosis. The VKR is a marketing term for Swedish Red, Danish Red, and Finnish Ayrshire breed which complicated breed determination. Therefore, genomic breed composition estimates were compared using two different representations of VKR, one of which was based on parents used in the crossing system and a second based on genotypes from the ancestral breeds that comprise VKR. Variation of breed composition estimates were assessed between pedigree and genome-based predictions. Lastly, Genomic estimations were correlated with production and health traits by comparing extreme performance groups to identify the relationship between breed ancestry and performance. With the exception of the JER breed composition in Grazecross, all other estimates of the purebred contribution to the ProCROSS and Grazecross showed a significant difference in their genomic breed estimation when using the VKR ancestral versus the VKR parental reference populations for admixture analysis. These observations were expected given the different relationship of each VKR representation to the crossbred cattle. Further analysis showed that regardless of which VKR reference population was used, the degree of MON and HOL breed composition plays a significant role in milk and fat production in ProCROSS, while the degree of VKR and NOR ancestry were related to improved health performance in Grazecross. In all, identifying the most appropriate and informative animals to use as reference animals in admixture analysis is an important factor when interpreting results of relationship and population structure, but some degree of uncertainty exists when assessing the relationship of breed composition to phenotypic performance.

The improvement of milk production of indigenous Sudanese cattle such as Bos indicus Butana and its cross with Holstein is a major goal of the Sudanese government to ensure sufficient healthy nutrition in the country. In this study, we investigated the K232A polymorphism of diacylglycerol acyltransferase (DGAT1), a well-known modulator of milk production in other breeds. We determined allele frequencies and the allele effects on milk production. Therefore, 93 purebred Butana and 203 Butana × Holstein crossbred cattle were genotyped using competitive allele-specific PCR assays. Association analysis was performed using a linear mixed model in R. In purebred Butana cattle, the lysine DGAT1 protein variant K232, which is found to be associated with higher fat and protein contents, as well as higher fat yield was highly frequent at 0.929, while its frequency in Butana × Holstein crossbred cattle was 0.394. Significant effects were found on milk yield (P = 7.6 × 10−20), fat yield (P = 2.2 × 10−17), protein yield (P = 2.0 × 10−19) and lactose yield (P = 4.0 × 10−18) in crossbred cattle. As expected, the protein variant K232 was disadvantageous since it was decreasing milk, protein, and lactose yields by 1.741 kg, 0.063 kg and 0.084 kg, respectively. No significant effects were found for milk fat, protein, and lactose contents. The high frequency of the lysine DGAT1 protein variant K232 in Butana cattle could contribute to their high milk fat content in combination with low milk yield. In Butana × Holstein crossbred cattle, the DGAT1 marker can be used for effective selection and thus genetic improvement of milk production. [ABSTRACT FROM AUTHOR]

Dietary protein adjustments can reduce environmental impact and economic losses in production systems. However, we lack information regarding nitrogen (N) metabolism and protein requirements for maintenance of crossbred animals such as Red Norte breed, precluding a precise dietary management. The objective was to evaluate the effect of increasing dietary CP levels (9%, 11%, 13%, 15% and 17%) on intake, digestibility and N balance, as well as to estimate the metabolizable protein requirements for maintenance (MPm) of growing Red Norte bulls. Thirty five animals averaging 280 ± 4.0 kg BW were fed during 45 days in a 60 : 40 forage : concentrate ratio diet in which the last 5 days were used for the digestibility trial. Intakes of CP and non-fibrous carbohydrates (NFCs) and feed efficiency linearly increased (P < 0.05) as CP levels increased, while DM, NDF, nitrogen efficiency use and ether extract were not influenced by CP levels (P > 0.05). Digestibilities of DM, organic matter, ether extract, NFC and CP as well as metabolizable energy intake linearly increased (P < 0.05), and true digestibility of CP was not affected (P > 0.05) by treatments. Urinary N and retained N linearly increased (P < 0.05) with the increase in dietary N. The MPm were estimated as 4.46 g/BW0.75 and the efficiency of use of MPm was 0.673. In conclusion, obtained MPm requirements of growing Red Norte bulls are greater than the values reported in literature for Zebu cattle and dietary CP levels of 15% and 17% exhibited great responses for growing Red Norte cattle. However, a cost-benefit evaluation should be done before its use.

Summary: In the present study, we investigated the major histocompatibility complex (MHC)‐B haplotypes diversity of Sri Lankan indigenous chickens from three different geographical sites consisting of highly mixed populations using 90 SNPs in the MHC‐B region. A total of 48 haplotypes were identified. Those included 37 novel haplotypes and 11 previously identified 'standard' haplotypes. The MHC‐linked marker, LEI0258, had 23 alleles showing less diversity than defined by MHC‐B SNP haplotypes. Among those identified haplotypes, five standard haplotypes—BSNP‐O02, BSNP‐M01, BSNP‐A04, BSNP‐K03, BSNP‐T04—were most commonly observed, suggesting past introgression of imported breeds. Comparison of the MHC‐B haplotypes of Sri Lankan and four other global populations with previously defined haplotypes indicated the sharing of 23 standard haplotypes with common origins. Novel haplotypes are population‐specific and not shared among the geographical boundaries. Backyard indigenous chickens are unselected, highly crossbred, and generally thrive under dynamic environmental conditions. Hence free‐range production systems may be responsible for maintaining high diversity in the MHC‐B region with novel haplotypes. [ABSTRACT FROM AUTHOR]

A balanced, diverse gut microbiota is vital for animal health. The microbial population is shaped by multiple factors including genetic background and environment, but other determinants remain controversial. Numerous studies suggest that the dominant factor is genetic background while others emphasize the environmental factors. Here, we bred asexual hybridization queens (AHQs) of honeybees through nutritional crossbreeding (laid in Apis mellifera colony but bred in Apis cerana colony), sequenced their gut microbiome, and compared it with normally bred sister queens to determine the primary factor shaping the gut microbiota. Our results showed that the dominant genera in the gut microbiota of AHQs were Brevundimonas , Bombella , and Lactobacillus , and its microbial community was more related to A. mellifera queens. The AHQs had a moderate number of different bacterial species and diversity, but total bacterial numbers were low. There were more significant taxa identified in the comparison between AHQ and A. cerana queen according to LEfSe analysis results. The only genetic-specific taxon we figured out was Brevundimonas. The growth of core bacterial abundance showed different characteristics among different queen groups in the first week after emerging. Collectively, this study suggested that the genetic background played a more dominant role than environmental factors in shaping the gut microbiota of honeybee queen and the microbiota of midgut was more sensitive than that of rectum to this impact. [ABSTRACT FROM AUTHOR]

A balanced, diverse gut microbiota is vital for animal health. The microbial population is shaped by multiple factors including genetic background and environment, but other determinants remain controversial. Numerous studies suggest that the dominant factor is genetic background while others emphasize the environmental factors. Here, we bred asexual hybridization queens (AHQs) of honeybees through nutritional crossbreeding (laid in Apis mellifera colony but bred in Apis cerana colony), sequenced their gut microbiome, and compared it with normally bred sister queens to determine the primary factor shaping the gut microbiota. Our results showed that the dominant genera in the gut microbiota of AHQs were Brevundimonas, Bombella, and Lactobacillus, and its microbial community was more related to A. mellifera queens. The AHQs had a moderate number of different bacterial species and diversity, but total bacterial numbers were low. There were more significant taxa identified in the comparison between AHQ and A. cerana queen according to LEfSe analysis results. The only genetic-specific taxon we figured out was Brevundimonas. The growth of core bacterial abundance showed different characteristics among different queen groups in the first week after emerging. Collectively, this study suggested that the genetic background played a more dominant role than environmental factors in shaping the gut microbiota of honeybee queen and the microbiota of midgut was more sensitive than that of rectum to this impact.

The economic benefit of dairy products can be enhanced by increasing the efficiency of growth and survival rate of calves. The objective of the study was to evaluate the effects of non-genetic factors on pre- and post-weaning growth traits and mortality rate of pure Fogera and their F1 cross Fogera × Holstein Friesian calves at the Andassa and Chagni Cattle Breeding and Improvement Ranch. Retrospective types of study design and direct measurement were used to collect the data. A total of 3,626 for pre-weaning weight records and 107 for post-weaning measurements were used for analysis. Genotype, sex, dam parity, year of birth, the season of birth, and location were the considered production factors. The overall least square means (LSM ± SE) of birth weights were 22.33 ± 0.12 and 24.56 ± 0.11 kg for Fogera and their crosses, respectively. The overall LSM ± SE of weaning weights were 93.25 ± 0.85 and 111.63 ± 0.84 kg for Fogera and their cross calves, respectively. The overall post-weaning weight for Fogera and their cross calves were 101.02 ± 1.62 and 111.08 ± 2.65, respectively. All variables considered in the model, except dam parity, significantly (P < 0.05) affected post-weaning weight. The overall recorded mortality rate was 3% which was nearly the optimum that many scholars agreed to successful rearing practices.

The primary purpose of the current study was to assess the genetic diversity, runs of homozygosity (ROH) and ROH islands in a Chinese composite pig and explore hotspot regions for traces of selection. First, we estimated the length, number, and frequency of ROH in 262 Xidu black pigs using the Porcine SNP50 BeadChip and compared the estimates of inbreeding coefficients, which were calculated based on ROHs (FROH) and homozygosity (FHOM). Our result shows that a total of 7,248 ROH exceeding 1Mb were detected in 262 pigs. In addition, Sus scrofa chromosome (SSC) 8 and SSC10, respectively, has the highest and lowest chromosome coverage by ROH. These results suggest that inbreeding estimation based on total ROH may be a useful method, especially for crossbreed or composite populations. We also calculated an inbreeding coefficient of 0.077 from the total ROH. Eight ROH islands were found in this study. These ROH islands harbored genes associated with fat deposition, muscular development, reproduction, ear shape, and adaptation, such as TRAF7, IGFBP7, XPO1, SLC26A8, PPARD, and OR1F1. These findings may help to understand the effects of environmental and artificial selection on the genome structure of composite pigs. Our results provide a basis for subsequent genomic selection (GS), and provides a reference for the hybrid utilization of other pig breeds.

The primary purpose of the current study was to assess the genetic diversity, runs of homozygosity (ROH) and ROH islands in a Chinese composite pig and explore hotspot regions for traces of selection. First, we estimated the length, number, and frequency of ROH in 262 Xidu black pigs using the Porcine SNP50 BeadChip and compared the estimates of inbreeding coefficients, which were calculated based on ROHs (FROH) and homozygosity (FHOM). Our result shows that a total of 7,248 ROH exceeding 1Mb were detected in 262 pigs. In addition, Sus scrofa chromosome (SSC) 8 and SSC10, respectively, has the highest and lowest chromosome coverage by ROH. These results suggest that inbreeding estimation based on total ROH may be a useful method, especially for crossbreed or composite populations. We also calculated an inbreeding coefficient of 0.077 from the total ROH. Eight ROH islands were found in this study. These ROH islands harbored genes associated with fat deposition, muscular development, reproduction, ear shape, and adaptation, such as TRAF7 , IGFBP7 , XPO1 , SLC26A8 , PPARD , and OR1F1. These findings may help to understand the effects of environmental and artificial selection on the genome structure of composite pigs. Our results provide a basis for subsequent genomic selection (GS), and provides a reference for the hybrid utilization of other pig breeds. [ABSTRACT FROM AUTHOR]

Although artificial insemination in pig production is widespread, many farms rely on natural mating (NM). For NM to be successful, adequate sexual behaviour and a sufficient libido level in boars is crucial for appropriate reproductive performance. Therefore, this observational study evaluated the libido level of 59 boars of different breeds used for NM by a scoring system ranging from 0 (no libido) to a maximum of 23 points (perfect libido) and analysed their general and andrological health as well as housing and management factors influencing the libido. The boars showed a mean libido score of 16.7 (SD ± 2.3). Crossbreed (median: 17.5, min: 14.0, max: 21.0) (p < 0.01) and Landrace (median: 17.5, min: 17.0, max: 21.0) (p < 0.05) boars showed significantly higher libido scores compared to Piétrain boars (median: 14.0, min: 13.0, max: 17.0). A non-slippery mating area was found to be positively associated with libido (p < 0.05), whereas disorders such as lameness showed a negative correlation (p < 0.01). In winter, significantly higher libido values (median: 17.0, min: 14.0, max: 21.0) were recorded compared to spring (median: 16.0, min: 11.0, max: 21.0) (p < 0.05). In addition, high number of supports by farmers during natural mating and frequency of use for NM and for stimulation of artificially inseminated sows did not negatively influence the libido score. No significant correlation between the satisfaction of the farmer about the libido level of the boars and the evaluated libido score was detected. This is the first study investigating the effect of housing conditions and management procedures on the libido of boars used for NM under field conditions. The findings may therefore help to establish a successful mating management for herds applying NM on a regular basis. A systematic evaluation of the libido score is needed to detected boars with low libido levels, because it is often overlooked by the farmers. Moreover, genetics, general health condition and flooring in mating areas turned out to be critical parameters that influence the libido score of boars used for NM and therefore should be considered when examining natural mating on farm. • Boars for natural mating showed a high libido score. • Flooring influenced the libido of boars. • Genetics had a influence of the libido score. • In winter boars showed higher libido compared to spring. [ABSTRACT FROM AUTHOR]

Poor semen quality and infertility/subfertility are more frequent in crossbred than zebu bulls. Using a highthroughput liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based approach, we established the preliminary metabolomic profile of crossbred and zebu bull spermatozoa (n=3 bulls each) and identified changes in sperm metabolomics between the two groups. In all, 1732 and 1240 metabolites were detected in zebu and crossbred bull spermatozoa respectively. After excluding exogenous metabolites, 115 and 87 metabolites were found to be unique to zebu and crossbred bull spermatozoa respectively whereas 71 metabolites were common to both. In the normalised data, 49 metabolites were found to be differentially expressed between zebu and crossbred bull spermatozoa. The significantly enriched (P<0.05) pathways in spermatozoa were taurine and hypotaurine metabolism (observed metabolites taurine and hypotaurine) in zebu and glycerophospholipid metabolism (observed metabolites phosphatidylcholine, phosphatidylethanolamine and phosphatidylserine) in crossbred bulls. The abundance of nitroprusside (variable importance in projection (VIP) score >1.5) was downregulated, whereas that of L-cysteine, acetyl coenzyme A and 2'-deoxyribonucleoside 5'-diphosphate (VIP scores>1.0) was upregulated in crossbred bull spermatozoa. In conclusion, this study established the metabolomic profile of zebu and crossbred bull spermatozoa and suggests that aberrations in taurine, hypotaurine and glycerophospholipid metabolism may be associated with the higher incidence of infertility/subfertility in crossbred bulls. [ABSTRACT FROM AUTHOR]

Somba cattle is one of the two trypanotolerant West-African Dwarf (WAD) breeds. Crosses between taurine (Bos taurus) and Zebu (Bos indicus) breeds are reported in various localities in West-Africa. The aim of this study was to assess the birth weight, the milk production and the mortality rate in crossbred cattle (Dwarf taurine x Zebu) in Togo. The study was carried out between 2015 and 2017 in February (dry season), May and September (rainy season) of each year in two Prefectures (Mô and Tchaoudjo) located in Central Administrative region of Togo. A total of 207 calves and 290 milking cows were randomly sampled for the study. Calves consisted of 72 WAD, 67 Zebu and 68 crossbred (WAD x Zebu) phenotypically taurine. The male calves weigh 17.70 ± 2,6 kg against 16.59 ± 1.97 kg for the female in crossbreeds; 13.76 ± 1.07 kg and 12.37 ± 1.16 kg in WAD and 22.55 ± 3.34 kg and 19.66 ± 2.15 kg in Zebus cattle. The milk production was significantly higher in the crossbreed (0.68 ± 0.27 L) than in WAD (0.46 ± 0.15 L) on the one hand and it was influenced by the rank of calving (P = 0.000) on the other hand. The mortality rates were 10.98 ± 3.41%; 11.85 ± 4.19% and 12.17 ± 5.06% in Crossbred, WAD and zebus respectively and no significant difference was observed among the three phenotypes. However, the calving-season had significant effect (P = 0.003) on the mortality rates. In the light of the foregoing figures, we come to conclusion that Somba breed performs far less compared to the crossbreed (Dwarf taurine x Zebu) and Zebus phenotypes in terms of birth weight and milk production. [ABSTRACT FROM AUTHOR]

This study aims to determine the performance of F2 resulting from crossing (Layer Stud vs. Hatcher resulting in pullet F1, F1 mated with Layer Male, resulting in F2). Performance of F2 given ration with levels of Crude Protein (CP) 16% and 18%, as well as “Herbs“. The research was carried out in a Completely Randomized Design 2 x 4 factorial pattern. Protein factors were 2 kinds of rations with Crude Protein levels of 16% and 18% of metabolic energy 3062.40 and 3013.20 kcal/kg. Herbs factor were doses of herbal dosage 0, 1, 2, 3 g/kg ration. Each treatment was repeated 4 times. Data collection performance were include rationing, giving protein, egg weight, and chicken day production, and mild shells. The results showed those the level of crude and herbal protein had no interaction, and the level of crude protein which was different was not significant (P> 0.05) on feed consumption and was significantly different (P 0.05) on feed consumption, ration crude protein, egg weight, and thick shell, but significantly different (P

Objective This experiment was conducted to determine the differences in the apparent ileal (AID) and total tract digestibility (ATTD) of nutrients and indispensable amino acids (IAA) in high-fiber diets with wheat middlings, rice bran or alfalfa meal fed to Duroc×(Landrace× Yorkshire) (DLY) and Duroc× (Berkshire×Jiaxing) (DBJ) growing barrows. Methods Eighteen DLY and 18 DBJ growing barrows were randomly allotted to a 2×3 factorial arrangement involving 2 crossbreeds and 3 high-fiber diets. The experiment lasted 15 d with 10 d for diets adaptation, 3 d for feces collection and 2 d for digesta collection. Three diets were based on corn and soybean meal with 25% wheat middlings, rice bran and alfalfa meal respectively. Results DBJ had a greater (p

The aim of this study was to evaluate growth ability (weight, daily weight gains) and selected carcass characteristics during fattening period of Wagyu × Aberdeen Angus crossbred steers (F1). A total of 72 animals were evaluated over a period of 4 years. The observed animals were monitored from rearing (8 months) to slaughter (30 months). Statistical evaluation was performed by SAS 9.3 (GLM procedure); variables were corrected for effects of the year, season of birth and sire effect. Sire effect proved to be the most significant in our evaluation. Offsprings sired by Bull 1 had significantly (P < 0.05) better growth ability than offsprings of other two tested bulls. Slaughter analysis of tested steers showed, that mean value of dressing percentage was 55.61 %. Significantly highest dressing percentage was found for Bull 1 offsprings (56.72 %, P < 0.05). The average carcass weight of tested steers was 443.46 kg and the highest carcass weight was again achieved by offsprings of Bull 1 (486.39 kg; P < 0.05). Average value of beef marbling score was 5.21. Slaughter analyses of carcass cuts (cut‑out, round, chuck, rump, tenderloin, shank, flank) and tallow showed that average weight of these lean cuts was 127.32 kg and tallow content was 39.31 kg at average. The highest values of these parameters were observed in offspring of bulls Bull 1 (P < 0.05).

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Dietary protein adjustments can reduce environmental impact and economic losses in production systems. However, we lack information regarding nitrogen (N) metabolism and protein requirements for maintenance of crossbred animals such as Red Norte breed, precluding a precise dietary management. The objective was to evaluate the effect of increasing dietary CP levels (9%, 11%, 13%, 15% and 17%) on intake, digestibility and N balance, as well as to estimate the metabolizable protein requirements for maintenance (MPm) of growing Red Norte bulls. Thirty five animals averaging 280 ± 4.0 kg BW were fed during 45 days in a 60 : 40 forage : concentrate ratio diet in which the last 5 days were used for the digestibility trial. Intakes of CP and non-fibrous carbohydrates (NFCs) and feed efficiency linearly increased (P < 0.05) as CP levels increased, while DM, NDF, nitrogen efficiency use and ether extract were not influenced by CP levels (P > 0.05). Digestibilities of DM, organic matter, ether extract, NFC and CP as well as metabolizable energy intake linearly increased (P < 0.05), and true digestibility of CP was not affected (P > 0.05) by treatments. Urinary N and retained N linearly increased (P < 0.05) with the increase in dietary N. The MPm were estimated as 4.46 g/BW0.75 and the efficiency of use of MPm was 0.673. In conclusion, obtained MPm requirements of growing Red Norte bulls are greater than the values reported in literature for Zebu cattle and dietary CP levels of 15% and 17% exhibited great responses for growing Red Norte cattle. However, a cost-benefit evaluation should be done before its use. [ABSTRACT FROM AUTHOR]

The aim of this study was to assess the distribution of runs of homozygosity (ROH) and autozygosity islands in the composite Montana Tropical® beef cattle to explore hotspot regions which could better characterize the different biological types within the composite breed. Montana animals (n = 1,436) were genotyped with the GGP‐LD BeadChip (~30,000 markers). ROH was identified in every individual using the plink v1.90 software. Medium and long ROH prevailed in the genome, which accounted for approximately 74% of all ROH detected. On an average, 2.0% of the genome was within ROH, agreeing with the pedigree‐based inbreeding coefficient. The Montana cattle with a higher proportion of productive breed types showed the highest number of autozygosity islands (n = 17), followed by those with a higher proportion of breeds adapted to tropical environments (n = 15). Enriched terms (p <.05) associated with the immune and inflammatory response, homeostasis, reproduction, mineral absorption, and lipid metabolism were described within the autozygosity islands. In this regard, over‐represented GO terms and KEGG pathways described in this population may play a key role in providing information to explore the genetic and biological mechanisms together with the genomic regions underlying each biological type that favoured their optimal performance ability in tropical and subtropical regions. [ABSTRACT FROM AUTHOR]

At present, one of the basic objectives of livestock production development in Kazakhstan is to provide the population with qualified, accessible meat. An important reserve for increasing the production of meat, in particular, beef, is the development of specialized meat breeding. The leading position in this industry in the Republic of Kazakhstan belongs to Kazakh white-headed breed, which was withdrawn in 1932-1950 with the way of breeding Kazakh cows and partially Kalmyk cattle with the bulls of Hereford breed. At present, this breed is raised almost in all regions of the Republic. The breed is characterized by good meat productivity, sufficient high early maturation and animals have excellent adaptation to continental climatic conditions. The aim of research is to study the results of meat productivity of bulls of different genotypes in conditions of LLP Agrofirm «Dinara- Ranch», Almaty region, the Republic of Kazakhstan. To achieve this goal, the following objectives have been put before us: - determination of slaughter weight and yield of morphological composition of carcasses and qualitative indicator of experimental bulls' meat; - to give an assessment of qualitative indicators of meat products obtained from the bulls of different genotypes. Meat obtained in control slaughtering from experimental bulls coincided requirements of I category. According to the weight of hot carcass Hereford bulls exceeded the peers of Kazakh whiteheaded breed to 42.9 kg or 18.1% (p > 0.95) and bulls crossbreed to 9.0 kg or 3.3% (p > 0.95). Yield of inner salo was higher in the bulls of Kazakh white-headed breed. Bulls of Hereford breed exceeded their Kazakh white-headed breed by the amount of meat per 43.2 kg -- 22.5% (p > 0.95) and bulls crossbreed to 8.1 kg -- 3.6% (p > 0.95). Yield of bulls meat of Hereford breed exceeded its peers of Kazakh white-headed breed to 2.4 -- 2.9% (p < 0.95), but conceded to crossbred bulls to 0.1 kg -- 0.1%. Correlation of protein and fat in the meat of experimental animals comprised: in the bulls of Kazakh white-headed breed - 1:0.65, in the animals of the Hereford breed and crossbreed - 1:0.64. [ABSTRACT FROM AUTHOR]

Iberian ham is a highly appreciated product and according to Spanish legislation different labels identify different products depending on the genetic purity. Consequently, "100% Iberian" ham from purebred Iberian animals is more expensive than "Iberian" ham from Iberian x Duroc crosses. The hypothesis of this study was that to avoid labelling fraud it is possible to distinguish the breed (Iberian or Iberian x Duroc) of acorn-fed pigs of Iberian ham without any prior preparation of the sample by using spectroscopy that is a rapid and reliable technology. Moreover, portable devices which can be used in situ could provide similar results to those of benchtop equipment. Therefore, the spectra of the 60 samples (24 samples of 100% Iberian ham and 36 samples of Iberian x Duroc crossbreed ham) were recorded only for the fat, only for the muscle, or for the whole slice with two benchtop near-infrared (NIR) spectrometers (Büchi NIRFlex N-500 and Foss NIRSystem 5000) and five portable spectrometers including four portable NIR devices (VIAVI MicroNIR 1700 ES, TellSpec Enterprise Sensor, Thermo Fischer Scientific microPHAZIR, and Consumer Physics SCiO Sensor), and one RAMAN device (BRAVO handheld). The results showed that, in general, the whole slice recording produced the best results for classification purposes. The SCiO device showed the highest percentages of correctly classified samples (97% in calibration and 92% in validation) followed by TellSpec (100% and 81%). The SCiO sensor also showed the highest percentages of success when the analyses were performed only on lean meat (97% in calibration and 83% in validation) followed by microPHAZIR (84% and 81%), while in the case of the fat tissue. Raman technology showed the best discrimination capacity (96% and 78%) followed by microPHAZIR (89% and 81%). Therefore, spectroscopy has proved to be a suitable technology for discriminating ham samples according to breed purity; portable devices have been shown to give even better results than benchtop spectrometers., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Authors.)

Crossbreeding can improve production performance and meat quality in sheep. The objective of this study was to look for genes related to sheep growth, development, and muscle. In this study, Dorper (DP) × Small Tailed Han (STH) sheep and Mongolia (MG) × Small-tailed Han (STH) sheep were used to estimate the productive performance and meat quality in a crossbreed. Subsequently, transcriptome analysis and bioinformatic analysis were performed on the Longissimus dorsi muscles of DP × STH and MG × STH sheep to identify differentially expressed genes (DEGs) related to growth, development, and meat quality. The presence of DEGs was confirmed by real-time PCR (qPCR). Productive performance and meat quality of the DP × STH sheep were better than the MG × STH sheep. Compared to DP × STH, a total of 1445 DEGs were identified in MG × STH sheep (1026 DEG were up-regulated and 419 DEG were down-regulated). Of these, 38 DEGs were related to growth, 161 to development, and 43 to muscle. In addition, 13 co-expressed genes (FGFRL1, SIX1, PLCB1, CRYAB, MYL2, ADIPOQ, GPX1, PPARD, GPC1, CDC42, LOC101106246, IGF1, and LARGE) were identified. The expression of DEGs was consistent with the comparative transcriptome analysis. This work provides genetics resources for future research on muscle development in sheep.

The objective of the present study was to test multivariate tools to evaluate the growth of Berganês and crossbreed in the Northeastern Semiarid region. Biometric measurements were performed at the beginning and at the end of the experiment in uncastrated male lambs belonging to the Berganês (BG), Berganês x Santa Inês (BSI), Dorper x Berganês (DB) genotypes and the Dorper x Santa Inês (DSI), of eight lambs per genotype. The design was completely randomized. Data were subjected to Person correlation analysis. It performed factor analysis based on principal components - (FA), in order to summarize the original set of variables in a few factors and to indicate the variables that most contribute to the evaluation of animal growth. A significant correlation (p < 0.01) was observed for most variables measured at the end of the experiment. The most important characteristics to evaluate the biometric growth of Berganês and crossbreed lambs are associated with the breed pattern and body development of the animals, according to factor analysis. The lambs were classified in their respective genotypes at the end of the experiment according to the discriminant analysis. The multivariate techniques used in this study were handy to understand and differentiate the Berganês genetic group and its crossbreed based on biometric characteristics. [ABSTRACT FROM AUTHOR]

The nutritional energy requirements of animals for maintenance and weight gain, such as the energy partition of the diet, were determined in different feeding plans. Fifteen F1 Holstein × Gyr, non-castrated male bovines with a mean initial liveweight of 302 kg were used. The diets were corn silage and concentrate, formulated to enable gains of 100, 500 and 900 g/day, called low, medium and high weight gains, respectively. Tests of digestibility and metabolism were conducted to determine energy losses through faeces, urine and methane emissions. Heat production was determined using respirometry chamber. Net energy for maintenance was calculated as the antilogarithm of the intercept of the regression of the logarithm of the heat production, as a function of the metabolisable energy intake. Retained energy was obtained by subtracting the heat production from the metabolisable energy intake. With the increased consumption of dry matter, there was an increase in faecal and urinary energy loss. Retained energy increased linearly with the metabolisable energy intake. The net energy for gain in the diet did not differ among the treatments, such as the efficiency of use of metabolisable energy for weight gain kg (0.34). The net energy for maintenance was 312 kJ/kg LW0.75, and the metabolisable energy for maintenance was 523 kJ/kg LW0.75. The daily methane production (g/day) increased with the dry matter level and the daily loss represented 5.31% of the gross energy consumption. The determination of the nutritional energy requirements for bovines of different genetic groups and under different feeding conditions allows the appropriate adjustment of feed formulation for each animal category. Knowledge of the energy partition of the diet and methane production allows the development of appropriate nutritional strategies respecting the environment. [ABSTRACT FROM AUTHOR]

This study aimed to evaluate clinical and biochemical changes in dairy cows during periparturient period. It was carried out on 38 multiparous Holstein/Fersian crossbreed dairy cows 3–11 years old. Using ultrasonography for measurement backfat thickness (BFT) for detection of the energy deficit. BFT on dairy cattle is used to monitor changes in subcutaneous fat thickness which represent changes in energy stores. On the basis of clinical examination and assessment of BHBA concentration in blood, cows were classified to four groups, control healthy cows (C = 16), subclinical ketosis (SCK = 10), cows with retained placenta (RP = 6), and clinical mastitis (CM = 6). BFT showed a significant decrease (p < 0.05) after parturition in all diseased groups compared with healthy. The plasma level of NEFA and BHBA showed a significant increase (p < 0.05) at 2 weeks a.p (antepartum) and 2 weeks p.p (postpartum) in RP and SCK than other groups, respectively. The plasma level of glucose showed a significant decrease (p < 0.05) at 2 weeks p.p in SCK group. The plasma level of insulin showed a significant decrease (p < 0.05) at 4 weeks a.p in RP group and in all groups after parturition. It could be concluded that production diseases induced clinical, biochemical, and subcutaneous fat changes in dairy cows. [ABSTRACT FROM AUTHOR]