Author: "Cristina Lencioni" - Searchworks@Jio Institute Digital Library Search Results

46 results on '"Cristina Lencioni"'

1. 1-Hour OGTT Plasma Glucose as a Marker of Progressive Deterioration of Insulin Secretion and Action in Pregnant Women

Author: Alessandra Ghio, Giuseppe Seghieri, Cristina Lencioni, Roberto Anichini, Alessandra Bertolotto, Alessandra De Bellis, Veronica Resi, Emilia Lacaria, Stefano Del Prato, and Graziano Di Cianni
Subjects: Diseases of the endocrine glands. Clinical endocrinology, RC648-665
Abstract: Considering old GDM diagnostic criteria, alterations in insulin secretion and action are present in women with GDM as well as in women with one abnormal value (OAV) during OGTT. Our aim is to assess if changes in insulin action and secretion during pregnancy are related to 1-hour plasma glucose concentration during OGTT. We evaluated 3 h/100 g OGTT in 4,053 pregnant women, dividing our population on the basis of 20 mg/dL increment of plasma glucose concentration at 1 h OGTT generating 5 groups (
Published: 2012
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2. Avaliação hematológica, bioquimica e ultrassonográfica hepato-renal de caninos submetidos à terapia com Tiocolchicosídeos

Author: Lianna Fernandes Carvalho, Luiz Fernando Jantzen Gaspar, Luzia Cristina Lencioni Sampaio, Jessica Paola Salame, Marta Zielke, Tatiane Schmitt Tavares, Sergiane Baes Pereira, and Risciela Salardi Alves de Brito
Subjects: cães, hematologia, enzimas hepáticas, enzimas renais, ultrassom, Veterinary medicine, SF600-1100
Abstract: O tiocolchicosídeo que é um derivado sulfuroso de um glicósido natural de colchicina, utilizado com miorrelaxante por ter ação menos sedativa do que outros relaxantes musculares que atuam sobre o SNC.. Esse medicamento é comumente utilizado no tratamento de contraturas musculares, espasmos sintomáticos, desordens reumáticas e neurológicas. O presente estudo foi realizado no Hospital de Clínicas Veterinária da Universidade de Pelotas com 16 cães hígidos. Foram realizados os exames hematológicos, bioquímicos e ultrassonográficos antes e após cada aplicação intramuscular do tiocolchicosídeo. Como resultado do estudo realizado, pode-se verificar que o tiocolchicosídeo na dose de 0,06mg/kg em cães, não ocasionou alterações nos parâmetros avaliados e não mostrou efeitos adversos relevantes nos animais em estudo. Nossos resultados concluem que o principio ativo estudado, na dose, frequência de administrações e período de tratamento, não mostrou toxicidade para os animais em experimento.
Published: 2018
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3. LINFOMA CANINO - RELATO DE CASO

Author: Prestes, Natália Dias, primary, Hepper, Ive Francesca Troccoli, additional, and Sampaio, Luzia Cristina Lencioni, additional
Published: 2019
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4. Production, purification and therapeutic potential of egg yolk antibodies for treating Trypanosoma evansi infection

Author: Sampaio, Luzia Cristina Lencioni, Baldissera, Matheus Dellaméa, Grando, Thirssa Helena, Gressler, Lucas Trevisan, Capeleto, Dianni de Menezes, de Sa, Mariângela Facco, de Jesus, Francielli Pantella Kuns, dos Santos Junior, Alceu Gonçalves, Anciuti, Andreia Nobre, Colonetti, Karina, Stainki, Daniel Roulim, and Monteiro, Silvia Gonzalez
Published: 2014
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5. Preventing type 2 diabetes and cardiovascular disease in women with gestational diabetes – The evidence and potential strategies

Author: Graziano Di Cianni, Veronica Resi, Cristina Lencioni, and Emilia Lacaria
Subjects: Pediatrics, medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, 030209 endocrinology & metabolism, Type 2 diabetes, Disease, 030204 cardiovascular system & hematology, Risk profile, 03 medical and health sciences, 0302 clinical medicine, Endocrinology, Pregnancy, Diabetes mellitus, Internal Medicine, medicine, Humans, Life Style, medicine.diagnostic_test, business.industry, Incidence, Incidence (epidemiology), General Medicine, medicine.disease, Gestational diabetes, Natural history, Diabetes, Gestational, Diabetes Mellitus, Type 2, Italy, Cardiovascular Diseases, Women's Health, Female, Lipid profile, business
Abstract: Gestational Diabetes Mellitus is a condition strongly related to the development of type 2 diabetes later in life, although the risk and the onset have not been fully identified yet. Although glucose tolerance returns to normal levels after delivery in the majority of women with GDM, this condition represents an early stage in the natural history of T2DM. In addition, women with previous GDM exhibit an increased cardiovascular risk profile and a raised incidence of cardiovascular diseases. Lifestyle changes and pharmacological interventions might be able to reduce the incidence of type 2 diabetes in pGDM women, although results are still not conclusive. Long term continuous programs specifically addressed to women with pGDM should be implemented, with the ambitious target to encourage them to regularly check glucose tolerance, lipid profile and other parameters related to cardiovascular diseases, aimed at improving women's health. In this paper, we review the relationship between type 2 diabetes, cardiovascular diseases and a history of GDM.
Published: 2018

6. Hepatotoxicidade do veneno botrópico (Bothrops jararaca)

Author: Luis Fernando Jantzen Gaspar, Luzia Cristina Lencioni Sampaio, Cleuza Kuhn, Jéssica Maroneze Sziminski, Eduarda Aléxia Nunes Louzada Dias Cavalcanti, Daiser Paulo de Almeida Sampaio Junior, and Amanda Andersson Pereira
Subjects: lcsh:Veterinary medicine, lcsh:SF600-1100, General Medicine
Abstract: Os acidentes ofídicos são comuns na rotina clínico veterinária, ocorrendo tanto em animais de companhia quanto animais de produção. São descritos em todas as regiões do território brasileiro, sendo responsável por quadros clínicos graves e ocasionalmente o óbito. As características e efeitos dos venenos da maioria dos ofídios são bem descritas na literatura, sendo a ação hepatotóxica relatada em serpentes do gênero crotálico. No presente trabalho descrevemos um quadro de fotodermatite resultante de acidente com uma jararaca (Bothrops jararaca) em um bovino adulto, raça Nelore, criado na zona rural do município de Abelardo Luz (SC). As lesões surgiram duas semanas após a picada, mostraram evolução generalizada e regressão demorada. Os exames complementares mostraram alterações das enzimas hepáticas alguns dias após o acidente ofídico e foram relacionados ao quadro dermatológico apresentado. O paciente permaneceu sob observação e tratamento por um período de 120 dias, apresentando melhora clínica significativa e regressão total das lesões.
Published: 2018

7. Hypoglycemia in Diabetic Pregnancy

Author: Emilia Lacaria, Graziano Di Cianni, Cristina Lencioni, and Laura Russo
Subjects: medicine.medical_specialty, Type 1 diabetes, business.industry, Type 2 diabetes, Hypoglycemia, medicine.disease, Glucagon, Endocrinology, Epinephrine, Pharmacokinetics, Diabetes mellitus, Internal medicine, medicine, business, Diabetic pregnancy, medicine.drug
Abstract: Hypoglycemia is a major factor preventing patients with both type 1 and type 2 diabetes from achieving near-normal glycemia. The risk of hypoglycemia in diabetic patients is due to both the imperfect pharmacokinetics of current therapy that produces inappropriately high insulin concentrations and a failure in the physiological protective mechanism that limits the decrease in blood glucose concentrations. A single threshold value for plasma glucose concentration that defines hypoglycemia in diabetes cannot be assigned. The insufficient contribution of glucose to the brain with an associated cerebral reduction of oxygen causes neuroglycopenic symptoms. Glucose, in physiological conditions, provides 90% of the energy necessary for brain functioning, and the cerebral functions are totally dependent on the level of the circulating glucose. In pregnant women with intensively treated type 1 diabetes, consistent lower epinephrine and glucagon responses were found with respect to nondiabetic nonpregnant women.
Published: 2019

8. LINFOMA CANINO - RELATO DE CASO

Author: Luzia Cristina Lencioni Sampaio, Ive Francesca Troccoli Hepper, and Natália Dias Prestes
Published: 2019

9. Screening for gestational diabetes in Tuscany, Italy. A population study

Author: Giuseppe Seghieri, Elisa Gualdani, Cristina Lencioni, Cecilia Berni, Alessandra Meucci, Emilia Lacaria, Graziano Di Cianni, Paolo Francesconi, and Lorenzo Roti
Subjects: Adult, medicine.medical_specialty, endocrine system diseases, Endocrinology, Diabetes and Metabolism, 030209 endocrinology & metabolism, Body weight, 03 medical and health sciences, 0302 clinical medicine, Endocrinology, Pregnancy, Risk Factors, Diabetes mellitus, Internal Medicine, medicine, Humans, Mass Screening, 030212 general & internal medicine, Mass screening, Gynecology, Glucose tolerance test, medicine.diagnostic_test, Obstetrics, business.industry, nutritional and metabolic diseases, General Medicine, medicine.disease, Gestational diabetes, Diabetes, Gestational, Italy, Population study, Female, business
Abstract: Aims According to current Italian guidelines, only women at higher risk of gestational diabetes (GDM) are eligible for receiving a glucose tolerance test (OGTT) during pregnancy. This study evaluates the compliance to national guidelines, additionally proposing an algorithm able to identify women with GDM. Methods The study included 23,270 pregnant women aged >15 yr, resident in Tuscany, a region in central Italy, delivering in year 2015, identified by certificates of care at delivery. Eligibility to screening was evaluated by fulfillment to regional guidelines recommending a 2-h-75 g-OGTT only in presence of risk factors. Additionally, a validated algorithm is proposed to diagnose GDM from regional administrative databases. Results Glucose tolerance was tested in 79.2% of women. Eligibility criteria were fulfilled in 55.6% of OGTT performers and in 40.2% of those who didn’t perform any OGTT. Performers’ rate increased with age, body weight, higher education degree and in those followed by a well defined clinical setting. Housewives and students had a lower chance of receiving an OGTT. Algorithm identified GDM in 11% of women, ranging from 14% among eligible and 7% among non-eligible ones. Conclusion Selective screening for GDM is not being currently applied in Tuscany. Despite the percentage of eligible women by national guidelines was 55%, the rate of those who performed the screening was about 80%, with a 40% of low risk women continuing to be screened. Moreover, GDM rate, calculated by algorithm, was relatively large (7%) among non-eligible women, suggesting the need of universal glucose screening to adequately capture all GDM cases.
Published: 2017

10. Prescribing exercise for prevention and treatment of gestational diabetes: review of suggested recommendations

Author: Gigliola Sabbatini, Cristina Bianchi, Roberto Miccoli, Pietro Bottone, Matilde Romano, Alessandra De Bellis, Stefano Del Prato, Cristina Lencioni, Alessandra Bertolotto, Mary Liana Mori, Lorella Battini, Anna Leopardi, Michele Aragona, Ilaria Cuccuru, Maria Calabrese, Serena Ottanelli, Maria Giovanna Salerno, Giuseppe Trojano, and Exercise
Subjects: Exercise, gestational diabetes, pregnancy, medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, Physical activity, 030209 endocrinology & metabolism, 03 medical and health sciences, 0302 clinical medicine, Endocrinology, Pregnancy, medicine, Humans, 030212 general & internal medicine, Medical prescription, Exercise, Uncomplicated pregnancy, Fetus, business.industry, Obstetrics and Gynecology, Medical evaluation, medicine.disease, Exercise Therapy, Gestational diabetes, Diabetes, Gestational, Treatment Outcome, Physical therapy, Female, gestational diabetes, business
Abstract: Exercise has been proved to be safe during pregnancy and to offer benefits for both mother and fetus; moreover, physical activity may represent a useful tool for gestational diabetes prevention and treatment. Therefore, all women in uncomplicated pregnancy should be encouraged to engage in physical activity as part of a healthy lifestyle. However, exercise in pregnancy needs a careful medical evaluation to exclude medical or obstetric contraindications to exercise, and an appropriate prescription considering frequency, intensity, type and duration of exercise, to carefully balance between potential benefits and potential harmful effects. Moreover, some precautions related to anatomical and functional adaptations observed during pregnancy should be taken into consideration. This review summarized the suggested recommendations for physical activity among pregnant women with focus on gestational diabetes.
Published: 2017

11. Production, purification and therapeutic potential of egg yolk antibodies for treating Trypanosoma evansi infection

Author: Daniel Roulim Stainki, Dianni Capeleto, Alceu Gonçalves dos Santos Junior, Lucas T. Gressler, Luzia Cristina Lencioni Sampaio, Karina Colonetti, Andreia Nobre Anciuti, Thirssa H. Grando, Silvia Gonzalez Monteiro, Matheus D. Baldissera, Mariângela F. de Sá, and Francielli Pantella Kuns de Jesus
Subjects: Trypanosoma, food.ingredient, Antibodies, Protozoan, Immune system, food, Western blot, Trypanosomiasis, Yolk, medicine, Animals, General Veterinary, biology, medicine.diagnostic_test, Inoculation, General Medicine, Trypanosoma evansi, biology.organism_classification, medicine.disease, Egg Yolk, Virology, Parasitology, biology.protein, Female, Antibody, Chickens
Abstract: The use of avian antibodies has aroused interest in biomedical research due to the numerous advantages compared to mammal's antibodies. Our study aimed to produce and purify IgY immunoglobulins in order to use as an alternative therapy against Trypanosoma evansi. Every 14 days, four New Hampshire chickens were immunized with trypomastigotes of T. evansi, totaling five inoculations. Eggs were collected during 70 days and the extraction of IgY was performed by precipitation through the PEG-6000 method. Characterization and purification of IgY anti-T. evansi were carried out by SDS-PAGE and Western blot, where heavy and light chains were detected. The production of IgY was noted during the whole period, and the average production was 2.87 ± 0.14 at the end of this study. Sample's titration allowed the quantification of specific IgY anti-T. evansi, with antibodies produced showing high avidity indexes. The results indicated that T. evansi is able to generate an immune response in poultry, resulting in a production of specific antibodies. In vivo test showed that IgY treatment resulted in increase of prepatent period, longevity and survival of infected animals, when compared with the positive control, demonstrating an initial, but no curative, trypanocidal activity.
Published: 2014

12. Hepatotoxicidade do veneno botrópico (Bothrops jararaca)

Author: Sampaio, Luzia Cristina Lencioni, primary, Gaspar, Luis Fernando Jantzen, additional, Sziminski, Jéssica Maroneze, additional, Pereira, Amanda Andersson, additional, Cavalcanti, Eduarda Aléxia Nunes Louzada Dias, additional, Kuhn, Cleuza, additional, and Sampaio Junior, Daiser Paulo de Almeida, additional
Published: 2018
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13. AVALIAÇÃO DO USO DE FÁRMACOS EM ANIMAIS DE COMPANHIA SEM ORIENTAÇÃO PROFISSIONAL

Author: Zielke, Marta, primary, Carvalho, Lianna Fernandes de, additional, Salame, Jéssica Paola, additional, Barboza, Daniele Vitor, additional, Gaspar, Luiz Fernando Jantzen, additional, and Sampaio, Luzia Cristina Lencioni, additional
Published: 2018
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14. Rivisitazione dei criteri di diagnosi del diabete gestazionale alla luce dei risultati dell’HAPO Study

Author: Graziano Di Cianni, Paolo Moghetti, Cristina Lencioni, and Veronica Resi
Subjects: media_common.quotation_subject, Art, Humanities, media_common
Abstract: Il diabete gestazionale (GDM) complica il 7–8% di tutte le gravidanze; se non diagnosticato e adeguatamente trattato, si associa a un’elevata morbilita materno-fetale riconducibile in primo luogo all’alterazione della crescita fetale. Nonostante la sua elevata incidenza e l’importante impatto clinico che lo contraddistingue, le modalita di diagnosi del GDM risultano ancora oggi oggetto di controversia. Non e ancora ben definito se per la sua individuazione debbano essere sottoposte a screening tutte le donne (screening universale) o solo quelle con determinati fattori di rischio (screening selettivo), ne tantomeno se la procedura di screening debba essere in fase unica o in due fasi, quale test di carico glucidico utilizzare (75 o 100 g?), quali i valori glicemici soglia. Per rispondere a questi interrogativi e stato disegnato un importante studio osservazionale a livello internazionale, lo studio HAPO (Hyperglycemia and Adverse Pregnancy Outcome Study), che ha coinvolto 25.000 donne con normale tolleranza glucidica in diversi paesi del mondo. I risultati dello studio hanno dimostrato in maniera inequivocabile che all’aumentare dei valori glicemici registrati, sia a digiuno che una e due ore dopo il carico glucidico, aumentava l’incidenza di tutti gli end-point primari dello studio (peso alla nascita oltre il 90° percentile, taglio cesareo, ipoglicemia neonatale, C-peptide neonatale elevato). Tuttavia, poiche l’associazione tra la glicemia e gli end-point dello studio ha mostrato un andamento lineare, lo studio non ha potuto stabilire valori glicemici soglia. Successivamente un panel di esperti a livello internazionale, partendo dai risultati dello studio HAPO ha elaborato nuove raccomandazioni per lo screening e la diagnosi del GDM. Come test diagnostico e stato scelto l’OGTT con 75 g. La nuova procedura ha avuto certamente il merito di semplificare quelle precedenti indicando in un solo test, l’OGTT a 75 g quello da utilizzare per la diagnosi. Allo stesso tempo, pero, la sua applicazione ha comportato importanti problematiche, in primo luogo legate al sensibile aumento dei casi di GDM. Pertanto le nuove raccomandazioni tardano a trovare l’approvazione da parte di importanti organismi scientifici internazionali e in Italia, dopo una prima fase di accettazione, si e proceduto ad una loro parziale riproposizione.
Published: 2012

15. Selective screening for GDM in Italy: application and effectiveness of National Guidelines

Author: Emilia Lacaria, Paolo Lemmi, Matilde Romano, Laura Russo, Cristina Lencioni, Stefano Del Prato, Alessandra Bertolotto, Graziano Di Cianni, and Lorella Battini
Subjects: Adult, medicine.medical_specialty, Pediatrics, Screening test, Pregnancy, Risk Factors, Prenatal Diagnosis, Epidemiology, Prevalence, medicine, Humans, Mass Screening, business.industry, Patient Selection, Public health, Obstetrics and Gynecology, Glucose Tolerance Test, medicine.disease, Gestational diabetes, Diabetes, Gestational, Italy, Practice Guidelines as Topic, Pediatrics, Perinatology and Child Health, Cohort, Gestation, Female, Guideline Adherence, business, Program Evaluation
Abstract: In September 2011 the Italian Public Health Authority established selective screening for GDM to be performed based on the presence of risk factors. In a cohort of 2552 Caucasian pregnant women we evaluated to which extent the new national guidelines (NGL) are correctly applied; moreover we estimated the prevalence of GDM assessed by NGL. Our data show that the NGL are still properly implemented since the screening test was performed in nearly the totality of the women at 24th and 28th week of gestation. GDM prevalence is 10.9%, 25% greater as compared to the one determined with the old criteria 10 years ago.
Published: 2014

16. AVALIAÇÃO DO USO DE FÁRMACOS EM ANIMAIS DE COMPANHIA SEM ORIENTAÇÃO PROFISSIONAL

Author: Marta Zielke, Lianna Fernandes de Carvalho, Luiz Fernando Jantzen Gaspar, Jéssica Paola Salame, Luzia Cristina Lencioni Sampaio, and Daniele Vitor Barboza
Subjects: General Medicine
Abstract: O uso de fármacos em animais de companhia sem orientação profissional inclui a prescrição realizada por pessoas não qualificadas, o uso de formulações caseiras e a automedicação orientada - quando o tutor reutiliza prescrições antigas. Este trabalho visou determinar a prevalência dos casos de medicação em animais de companhia sem orientação do médico veterinário, bem como identificar os fármacos mais utilizados, estabelecendo dados loco regionais referentes aos usuários do Hospital de Clínicas Veterinárias da Universidade Federal de Pelotas (HCV/UFPEL), situado na cidade de Capão do Leão - RS. A pesquisa foi realizada a partir de questionário fechado disponibilizado em sala de espera do HCV/UFPEL, e as questões abordadas incluíram o uso de medicações comerciais ou naturais sem orientação do médico veterinário, a indicação de leigos e o perfil dos tutores. Foram coletados dados de 267 animais, sendo 198 caninos e 69 felinos, sob a responsabilidade de 180 tutores. Vinte e um por cento das mulheres e 19% dos homens medicam seus animais sem orientação profissional com regularidade. Setenta e dois por cento dos tutores afirmaram comunicar ao veterinário que o animal foi previamente medicado, e 9% já presenciaram toxicidade medicamentosa relacionada ao uso de fármacos sem orientação veterinária. O estudo mostrou ser frequente o uso irracional de medicamentos em animais de companhia sem prescrição realizada pelo médico veterinário na população atendida pelo HCV/UFPEL.
Published: 2018

17. Normal Glucose Tolerance and Gestational Diabetes Mellitus

Author: Alessandra De Bellis, Alessandra Ghio, Cristina Lencioni, Federica Tesi, Graziano Di Cianni, Giuseppe Seghieri, Ilaria Cuccuru, Stefano Del Prato, L Volpe, and Roberto Anichini
Subjects: Advanced and Specialized Nursing, Normal glucose tolerance, medicine.medical_specialty, Pregnancy, Glucose tolerance test, endocrine system diseases, medicine.diagnostic_test, business.industry, Endocrinology, Diabetes and Metabolism, Insulin, medicine.medical_treatment, nutritional and metabolic diseases, medicine.disease, Gestational diabetes, Endocrinology, Diabetes mellitus, Internal medicine, Internal Medicine, medicine, Metabolic phenotype, Gestation, business, hormones, hormone substitutes, and hormone antagonists
Abstract: OBJECTIVE— The aim of this article was to define the metabolic phenotype of pregnant women with one abnormal value (OAV) during an oral glucose tolerance test (OGTT) and to test whether OAV could be considered metabolically comparable to gestational diabetes mellitus (GDM) or a specific entity between GDM and normal pregnancy. RESEARCH DESIGN AND METHODS— After 100-g 3-h OGTTs, 4,053 pregnant women were classified as having GDM, OAV, or normal glucose tolerance (NGT). Those with OAV were subdivided into three subgroups: fasting hyperglycemia (one abnormal value at fasting during an OGTT), 1-h hyperglycemia (one abnormal value at 1 h during an OGTT [1h-OAV]), or 2- or 3-h hyperglycemia (one abnormal value at 2 or 3 h during an OGTT). As derived from the OGTT, we measured insulin sensitivity (insulin sensitivity index [ISI] Matsuda) and insulin secretion (homeostasis model assessment for the estimation of β-cell secretion [HOMA-B], first- and second-phase insulin secretion). The product of the first-phase index and the ISI was calculated to obtain the insulin secretion–sensitivity index (ISSI). RESULTS— GDM was diagnosed in 17.9% and OAV in 18.7% of pregnant women; women with GDM and OAV were older and had higher BMI and serum triglyceride levels than those with NGT (all P < 0.05). Women with NGT had the highest ISI followed by those with OAV (−21.7%) and GDM (−32.1%). HOMA-B results were comparable with those for OAV and GDM but significantly (P < 0.01) lower than those for NGT; first- and second-phase insulin secretion appeared progressively reduced from that in women with NGT to that in women with OAV and GDM (P < 0.01). ISSI was higher in women with NGT than in women with either OAV (−34%) or GDM (−51.7%) (P < 0.001). Among OAV subgroups, the 1h-OAV subgroup showed the lowest ISSI (P < 0.05). CONCLUSIONS— OAV and GDM are clinically indistinguishable, and both groups are different from women with NGT. Women with GDM and OAV showed impaired insulin secretion and insulin sensitivity, although these defects are more pronounced in women with GDM. Compared with other OAV subgroups, 1h-OAV could be considered a more severe condition.
Published: 2007

18. Universal screening and intensive metabolic management of gestational diabetes: cost-effectiveness in Italy

Author: L Volpe, Antonio Boldrini, Luca Benzi, Di Cianni G, G Teti, Lorella Marselli, I Casadidio, Del Prato S, Bottone P, and Cristina Lencioni
Subjects: Adult, medicine.medical_specialty, endocrine system diseases, Cost effectiveness, Cost-Benefit Analysis, Endocrinology, Diabetes and Metabolism, Endocrinology, Pregnancy, Diabetes mellitus, Health care, Internal Medicine, medicine, Humans, Mass Screening, Mass screening, Retrospective Studies, business.industry, Obstetrics, Public health, nutritional and metabolic diseases, Retrospective cohort study, Health Care Costs, General Medicine, medicine.disease, female genital diseases and pregnancy complications, Surgery, Gestational diabetes, Diabetes, Gestational, Italy, Female, business
Abstract: This study retrospectively evaluated two groups of pregnant women. Group A women (n=1,338) were universally screened for gestational diabetes mellitus (GDM) and GDM patients were intensively treated. In Group B (n=4,035), screening was performed only in women at high risk for GDM and treatment was conventional. This study confirms the validity of a cost-effective screening program for the diagnosis of GDM and that selective screening may be an option only in a situation where healthcare resources are very scarce and/or universal screening of any kind is not feasible. Once the diagnosis of GDM has been made, metabolic management with an intensive approach is important to reduce maternal and fetal morbidity. Diagnosis of GDM and intensive treatment represent a cost for the public health system, but permit a significant monetary savings in terms of costs linked to maternal and neonatal morbidity.
Published: 2002

19. IgY immunotherapy in rats experimentally infected with Trypanosoma evansi

Author: Sampaio, Luzia Cristina Lencioni, Monteiro, Silvia Gonzalez, Stainki, Daniel Roulim, Silva, Aleksandro Schafer da, Ribeiro, Carmen Lucia Garcez, and Krause, Luciana Maria Fontanari
Subjects: Tripanosomose, Trypanosomosis, CIENCIAS AGRARIAS::MEDICINA VETERINARIA [CNPQ], IgY, Avian immunoglobulin, Imunoglobulina aviária
Abstract: Trypanosoma evansi is a protozoan flagellate that affect cattle, sheep, goats, donkeys, cats and pigs. The disease is particularly important in horses, known as "Mal das Cadeiras", due to deficits characteristic of locomotion in animals infected. The disease has a worldwide distribution, with several reports of natural infections in different regions in Brazil. Several pharmaceutical drugs have been recommended for the prophylaxis and control of the parasite, but over the years, these drugs have lost their effectiveness and protozoan looks developing resistance. The production and use of avian antibodies has aroused great interest in the scientific community because of the diversity of diagnostic and therapeutic applications. Due to the phylogenetic distance, immune diversification mechanisms and transferability of serum immunoglobulin to the egg yolk, currently recognizes a number of advantages to using avian antibodies rather than antibodies of mammals. This study produced a specific immunoglobulin against Trypanosoma evansi from immunization of chickens using an isolate of the parasite. After extraction and purification from egg yolk, cytotoxicity and genotoxicity of these antibodies in cultures of human leukocytes were evaluated. The therapeutic efficacy was tested in Rattus norvegicus. The trial also assessed the use of avian antibody associated with imidocarb dipropionate and diaceturate of diminazene. The toxicity tests showed that immunoglobulin does not produce gene and cell injury. In vivo tests showed increased in longevity and pre-patent period, mainly in rats treated with avian immunoglobulin associated with pharmacological treatment. The immunization and the extraction protocol used in this study allowed obtaining specific antibodies against protozoan and innocuous to the host. Moreover, when administered for therapeutic purposes, strengthens the immune system and prolongs the survival of infected patients. Trypanosoma evansi é um protozoário flagelado que acomete bovinos, ovinos, caprinos, asininos, felinos e suínos. A enfermidade tem especial importância em equinos, sendo conhecida como mal das cadeiras , devido aos déficits de locomoção característicos em animais infectados. A doença tem distribuição mundial, com vários relatos de infecções naturais em diversas regiões no Brasil. Várias drogas terapêuticas têm sido recomendadas para a profilaxia e controle do protozoário, porém ao longo dos anos estes medicamentos tem perdido a eficácia e o protozoário parece desenvolver resistência. A produção e uso de anticorpos aviários tem despertado grande interesse na comunidade científica devido à diversidade de aplicações diagnósticas e terapêuticas. Devido à distância filogenética, mecanismos de diversificação imune e capacidade de transferência das imunoglobulinas séricas para a gema do ovo, atualmente reconhece-se uma série de vantagens ao utilizar anticorpos aviários ao invés de anticorpos de mamíferos. Neste estudo foi produzido uma imunoglobulina específica contra o T. evansi a partir de imunização de galinhas usando um isolado do protozoário. Após a extração e purificação a partir da gema do ovo, foram avaliadas a citotoxicidade e genotoxicidade destes anticorpos em cultura de leucócitos humanos. A eficácia terapêutica foi testada em Rattus norvegicus. O ensaio também avaliou o uso do anticorpo aviário associado ao dipropionato de imidocarb e diaceturato de diminazene. Os testes de toxicidade mostraram que a imunoglobulina não produz lesões celulares e genéticas. Testes in vivo mostraram aumento da longevidade e período pré-patente, principalmente em ratos tratados com a imunoglobulina aviária associada ao tratamento farmacológico. O protocolo de imunização e extração usados nesta pesquisa permitiu a obtenção de anticorpos específicos contra o protozoário e inócuo para o hospedeiro. Além disso, ao ser administrado com fins terapêuticos, reforça o sistema imunológico e prolonga a sobrevida de pacientes infectados.
Published: 2014

20. Case report: Feline infection by Lagochilascaris sp. in the State of Rio Grande do Sul, Brazil

Author: Cíntia A. Denardin, Luzia Cristina Lencioni Sampaio, Silvia Gonzalez Monteiro, Daniel Roulim Stainki, Luciana Faccio, Camila B. Oliveira, Lucas T. Gressler, Alexandre A. Tonin, and Luciana Dalla Rosa
Subjects: Lagochilascaris, Male, Veterinary medicine, Ivermectin, General Veterinary, Antiparasitic Agents, education, Zoonosis, General Medicine, Biology, medicine.disease, Cat Diseases, Zoonotic disease, Ascaridida Infections, medicine, Cats, Helminths, Animals, Parasitology, Female, Felis catus, Ascaridida, Brazil
Abstract: Lagochilascariosis is a zoonotic disease caused by the nematode Lagochilascaris sp., with the northern of Brazil representing 81.2% of all reports of the disease worldwide. The aim of this study was to report the first occurrence of feline lagochilascariosis in the State of Rio Grande do Sul, southern of Brazil. It was diagnosed through coproparasitologic exam and laboratorial identification of the nematodes.
Published: 2012

21. Histopathology and ex vivo insulin secretion of pancreatic islets in gestational diabetes: A case report

Author: Matilde Masini, Piero Marchetti, Francesco Dotta, Graziano Di Cianni, Ugo Boggi, Mariella Tancredi, Marco Bugliani, Cristina Lencioni, Mara Suleiman, Franco Filipponi, Pellegrino Masiello, and Lorella Marselli
Subjects: Adult, endocrine system, medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Apoptosis, Acinar Cells, Biology, Arginine, Islets of Langerhans, Endocrinology, Fatal Outcome, Organ Culture Techniques, Pregnancy, Internal medicine, Diabetes mellitus, gestational diabetes, pancreatic islets, insulin secretion, apoptosis, regeneration, Glyburide, Insulin Secretion, medicine, Humans, Hypoglycemic Agents, Insulin, geography, geography.geographical_feature_category, Pancreatic islets, medicine.disease, Islet, Insulin oscillation, Gestational diabetes, Diabetes, Gestational, medicine.anatomical_structure, Glucose, Female, Ex vivo
Abstract: Gestational diabetes (GD) results from insufficient endogenous insulin supply. No information is available on features of islet cells in human GD. Herein, we describe several properties of islets from a woman with GD. Immunohistochemical stainings and EM analyses were performed on pancreatic samples. Islet isolation was achieved by enzymatic dissociation and density gradient centrifugation. Ex vivo insulin secretion was studied in response to fuel secretagogues. Control islets were obtained from matched non-pregnant, non-diabetic women. Total insulin positive area was lower in GD, mainly due to the presence of smaller islets. β-cell apoptosis and the presence of Ki67 positive islet cells were similar in GD and controls, whereas the amount of insulin positive cells in or close to the ducts was decreased in GD. Ex vivo insulin secretion did not differ between GD and non-pregnant, non-diabetic islets. These findings suggest that in this case of human GD there might mainly be a defect of β-cell amount, not due to increased apoptosis, but possibly to insufficient regeneration.
Published: 2011

22. Glucose variability in diabetic pregnancy

Author: Maria Grazia Dalfrà, Cosimo Scuffi, Jolanda Sposato, Graziano Di Cianni, Francesco Valgimigli, Cristina Lencioni, Giovanni Sartore, Serena Ottanelli, Giorgio Mello, Annunziata Lapolla, and Marco Scalese
Subjects: Adult, Blood Glucose, medicine.medical_specialty, endocrine system diseases, Endocrinology, Diabetes and Metabolism, Birth weight, Pregnancy in Diabetics, Endocrinology, Interquartile range, Pregnancy, Blood Glucose Self-Monitoring, Internal medicine, Diabetes mellitus, Medicine, Birth Weight, Humans, Glycemic, Type 1 diabetes, business.industry, Obstetrics, Infant, Newborn, nutritional and metabolic diseases, medicine.disease, Medical Laboratory Technology, Diabetes, Gestational, Diabetes Mellitus, Type 1, Linear Models, Female, business, Body mass index
Abstract: Fetal overgrowth is the most important complication of gestational (GDM) and pregestational diabetes mellitus.We correlated maternal glucose profiles, as detected by continuous glucose monitoring (CGM), with fetal growth parameters for 80 pregnant women (32 with type 1 diabetes, 31 with GDM, and 17 healthy controls). Glucose profiles were monitored in the first, second, and third trimesters of pregnancy for type 1 diabetes women and in the second and third trimesters for GDM women and controls. To analyze glycemic variability, we considered the mean amplitude of glycemic excursion, mean glycemia, the continuous overlapping net glycemic action (CONGA), the SD, the High Blood Glucose Index (HBGI), the Low Blood Glucose Index, and the interquartile range (IQR).Mean age was the same for the three groups. Prepregnancy body mass index was higher for the women with diabetes (GDM and type 1) than for controls. The newborn's mean birth weight and ponderal index were higher, although not significantly so, for the women with diabetes than for controls. For the type 1 diabetes patients, ponderal index correlated with the HBGI in the first trimester, CONGA1 and IQR in the second, and mean glycemia and SD in the third. For GDM patients, ponderal index correlated with mean glycemia and the HBGI in the second trimester.Fetal exposure to glycemic variability and hyperglycemia seems to be important in determining fetal overgrowth in pregnant women with diabetes. Optimal glucose control and less glucose variability are needed as early as possible in both type 1 diabetes and GDM patients to ensure normal fetal growth.
Published: 2011

23. Circulating endothelial progenitor cells in women with gestational alterations of glucose tolerance

Author: Cristina Lencioni, Maria Carla Iorio, Graziano Di Cianni, E. Storti, Laura Pucci, Renato Vanacore, Giuseppe Penno, Veronica Resi, Stefano Del Prato, and Daniela Lucchesi
Subjects: Adult, Blood Glucose, medicine.medical_specialty, Angiogenesis, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Antigens, CD34, Gestational Age, Impaired glucose tolerance, Glucose Metabolism Disorder, Pregnancy, Internal medicine, Diabetes mellitus, Internal Medicine, medicine, Humans, Glucose Metabolism Disorders, Glucose tolerance test, Analysis of Variance, Chi-Square Distribution, medicine.diagnostic_test, business.industry, Insulin, Stem Cells, Endothelial Cells, Kinase insert domain receptor, Glucose Tolerance Test, medicine.disease, Flow Cytometry, Vascular Endothelial Growth Factor Receptor-2, Gestational diabetes, Diabetes, Gestational, Endocrinology, Italy, cardiovascular system, Female, Cardiology and Cardiovascular Medicine, business
Abstract: Endothelial progenitor cells (EPCs) play a role in angiogenesis during pregnancy. The aim of this study was to evaluate circulating EPCs in pregnant women with gestational alterations of glucose tolerance. Glucose tolerance, insulin sensitivity and β-cell function were derived from oral glucose tolerance tests in 23 women with normal glucose tolerance (NGT), 18 with gestational impaired glucose tolerance (GIGT) and 24 with gestational diabetes mellitus (GDM). Circulating cells expressing CD34 in combination with CD133, kinase insert domain receptor (KDR) or both were quantified by flow cytometry. Women with GIGT and GDM had lower CD34+KDR+ and CD34+CD133 +KDR+ cells at 27±3.2 weeks’ gestation compared with NGT (ANOVA p+KDR+ and CD34+CD133+KDR+ cells were inversely correlated with the area-under-the-glucose-curve ( p
Published: 2011

24. Does nuchal translucency thickness in the first trimester predict GDM onset during pregnancy?

Author: Carlo Luchi, Chiara Nanini, Cristina Lencioni, Graziano Di Cianni, Martina Schifano, and Andrea R. Genazzani
Subjects: Adult, medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, Hospitals, University, Endocrinology, Predictive Value of Tests, Pregnancy, Nuchal Translucency Measurement, medicine, Humans, Increased nuchal translucency, Retrospective Studies, Gynecology, Fetus, Obstetrics, business.industry, Obstetrics and Gynecology, Retrospective cohort study, Glucose Tolerance Test, medicine.disease, Gestational diabetes, Diabetes, Gestational, Pregnancy Trimester, First, Early Diagnosis, Predictive value of tests, Pregnancy Trimester, Second, Gestation, Female, business
Abstract: This study was planned to evaluate whether increased nuchal translucency (NT) thickness in the first trimester of gestation can be related to onset of gestational diabetes mellitus (GDM) during pregnancy.From January 2006 to August 2008, a group of 678 singleton pregnancies who had developed GDM has been selected as a study group among a total of 3966 pregnant women who had undergone first trimester screening for aneuploidies at 11-14 weeks of gestation. A group of 420 single pregnant women with physiological pregnancy were enrolled as control group. Both fetal structural and karyotype's anomalies were excluded in the two groups. NT was mesured by a Fetal Medicine Foundation certificated operator; GDM was diagnosed at 24-28 weeks of gestation following Carpenter and Coustan criteria. In the analyses of continuos variables, study and control group were compared by Student's t-test and Anova test.There was no significative difference (p = 0.585) between NT values in the study (mean = 1.56) and control group (mean = 1.54).NT thickness does not show a significative increase in those women who subsequently develop GDM. Therefore, NT assessment does not prove to be an useful ultrasound parameter for predicting GDM onset during pregnancy.
Published: 2011

25. Physical activity and dietary habits during pregnancy: effects on glucose tolerance

Author: Alessandra Calianno, Alessandra Ghio, Luca Benzi, Graziano Di Cianni, Cristina Lencioni, L Volpe, Stefano Del Prato, Maria Cristina Pugliese, Alessandra Bertolotto, M. Corfini, and Veronica Resi
Subjects: Adult, Blood Glucose, medicine.medical_specialty, Pregnancy Trimester, Third, MEDLINE, Physical activity, Physiology, Motor Activity, Body Mass Index, Pregnancy, Internal medicine, Surveys and Questionnaires, Glucose Intolerance, medicine, Humans, Glucose tolerance test, medicine.diagnostic_test, business.industry, Obstetrics and Gynecology, Feeding Behavior, Glucose Tolerance Test, medicine.disease, Pregnancy Complications, Endocrinology, Pregnancy Trimester, Second, Pediatrics, Perinatology and Child Health, Observational study, Female, business, Energy Intake, Body mass index
Abstract: We designed this study to assess the potential effects of physical activity and dietary habits on glucose tolerance during pregnancy.This is an observational study involving 268 women who underwent a 50-g oral glucose challenge test (GCT) at 27±6.9 week of gestation. Plasma glucose level at 1-h GCT ≥ 140 mg/dl was used to define abnormal glucose tolerance (AGT). Physical activity was evaluated using the short form of the International Physical Activity Questionnaire (IPAQ), while for dietary habits we used a food frequency questionnaire linked to a computerised program.One hundred five women had AGT (AGT+) and 163 had normal glucose tolerance (AGT−). There was no difference between the two groups in demographic and clinical data, with the exception of pre-pregnancy BMI and weight gain both higher in AGT+ women. Also, all parameters referring to physical activity energy and diet (Kcal and diet components) were not statistically different between the two groups. After a multivariate analysis, only pre-pregnancy BMI (F-value 9.264, p=0.002) remained an independent predictor of 1-h plasma glucose.Our study suggests that high pre-pregnancy BMI confers a substantially high risk of AGT, independently of lifestyle during pregnancy.
Published: 2010

26. Continuous subcutaneous insulin infusion and multiple dose insulin injections in Type 1 diabetic pregnant women: a case-control study

Author: Alessandra Ghio, Alessandra Bertolotto, Cristina Lencioni, Veronica Resi, L Volpe, Stefano Del Prato, Michele Aragona, Graziano Di Cianni, Francesca Pancani, and Lorella Battini
Subjects: medicine.medical_specialty, medicine.medical_treatment, Birth weight, Endocrinology, Diabetes and Metabolism, Injections, Subcutaneous, Pregnancy in Diabetics, Infusions, Subcutaneous, Endocrinology, Pregnancy, Internal medicine, medicine, Birth Weight, Humans, Insulin, Glycemic, Retrospective Studies, Glycated Hemoglobin, Type 1 diabetes, Chi-Square Distribution, business.industry, Infant, Newborn, Gestational age, Obstetrics and Gynecology, Diabetic retinopathy, medicine.disease, Diabetes Mellitus, Type 1, Case-Control Studies, Gestation, Female, business
Abstract: The aim of this study was to evaluate the effects of continuous subcutaneous insulin infusion (CSII) on glycemic control and pregnancy outcomes in Type 1 diabetic pregnant women. We retrospectively evaluated 42 subjects, 20 treated with CSII and 22 with multiple dose insulin injections (MDI). The two groups were comparable for age, pre-pregnancy BMI, and primiparous rate, whereas women in the CSII group showed a tendency toward a longer diabetes duration (p = 0.06). Pre-pregnancy diabetic retinopathy and/or nephropathy were present in nine women of CSII and three of MDI. In all women metabolic control improved during pregnancy, without differences between the two groups and at the end of gestation HbA1c was 6.3 +/- 0.6 in CSII and 6.1 +/- 1.1% in MDI. Moreover, there were no differences in weight gain, whereas insulin requirement resulted significantly (p = 0.009) lower in CSII than in MDI. We recorded only one severe hypoglycaemic episode in both groups. No cases of deteriorations of the chronic diabetic complications were observed. The delivery occurred at 36.4 +/- 2.2 weeks; birth weight, the rate of large for gestational age, and the parameters of foetal morbidity were similar in both groups. In conclusions, CSII and MDI are both effective in improving maternal glucose control and have both similar pregnancy outcomes.
Published: 2009

27. Early subclinical atherosclerosis in women with previous gestational diabetes mellitus

Author: Alessandra Ghio, L Volpe, Graziano Di Cianni, Giuseppe Penno, Carmen Fotino, Alessandra Bertolotto, Ilaria Cuccuru, Stefano Del Prato, Luca Benzi, Vinicio Napoli, and Cristina Lencioni
Subjects: Adult, medicine.medical_specialty, Time Factors, Systole, Endocrinology, Diabetes and Metabolism, Population, Type 2 diabetes, Gastroenterology, Insulin resistance, Diastole, Pregnancy, Internal medicine, Diabetes mellitus, Internal Medicine, Medicine, Body Size, Humans, education, Advanced and Specialized Nursing, education.field_of_study, medicine.diagnostic_test, Adiponectin, business.industry, Glucose Tolerance Test, medicine.disease, Atherosclerosis, Gestational diabetes, Diabetes, Gestational, Endocrinology, Diabetes Mellitus, Type 2, Female, Metabolic syndrome, business, Lipid profile
Abstract: To determine if women with previous gestational diabetes mellitus (pGDM), a population at high risk for type 2 diabetes and metabolic syndrome (1), have signs of subclinical atherosclerosis, we measured carotid intimal-medial thickness (IMT) and multiple cardiovascular risk factors in 28 women with and 24 without pGDM (control group) 2 years after delivery. A 75-g 2-h oral glucose tolerance test was performed for assessment of glucose tolerance, area under the glucose curve (AUCgluc), insulin sensitivity index, homeostasis model assessment of insulin resistance (HOMA-IR), lipid profile, oxidized LDL (oxLDL), C-reactive protein (CRP), adiponectin, and fibrinogen. Family history, anthropometric parameters, and blood pressure were recorded. IMT was measured at four segments of …
Published: 2008

28. beta-Cell Failure in Type 2 Diabetes Mellitus

Author: Roberto Lupi, Cristina Lencioni, and Stefano Del Prato
Subjects: Amyloid, medicine.medical_specialty, endocrine system diseases, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Cell, Insulin resistance, Insulin-Secreting Cells, Internal medicine, Diabetes mellitus, Internal Medicine, medicine, Animals, Humans, Overeating, Glycemic, business.industry, Insulin, nutritional and metabolic diseases, Type 2 Diabetes Mellitus, medicine.disease, Lipids, Glucose, Endocrinology, medicine.anatomical_structure, Diabetes Mellitus, Type 2, Lipotoxicity, business
Abstract: Diabetes mellitus has been defined as a "group of metabolic diseases characterized by hyperglycemia resulting from defects in insulin secretion, insulin action, or both" and encompasses a wide range of heterogeneous conditions. Common type 2 diabetes mellitus (T2DM) results from a combination of genetic and acquired factors. However, lifestyle factors, particularly overeating and physical inactivity, are the major clinical determinants of T2DM. Insulin resistance is a common feature of T2DM, but it is unlikely to cause T2DM unless progressive loss of beta-cell function develops. Significant reduction in beta-cell function is already present at the time of T2DM diagnosis, and it continuously declines irrespective of treatment. As such, the progressive loss of beta-cell function dictates the rate of worsened glycemic control. Development of progressive deterioration accelerates via gluco- and lipotoxicity, loss of beta-cell function, and shrinkage of beta-cell mass. Understanding the causes for beta-cell failure is therefore of capital importance to develop new and more effective therapeutic strategies.
Published: 2008

29. Normal glucose tolerance and gestational diabetes mellitus: what is between?

Author: Graziano, Di Cianni, Giuseppe, Seghieri, Cristina, Lencioni, Ilaria, Cuccuru, Roberto, Anichini, Alessandra, De Bellis, Alessandra, Ghio, Federica, Tesi, Laura, Volpe, and Stefano, Del Prato
Subjects: Adult, Blood Glucose, Diabetes, Gestational, Pregnancy, Humans, Female, Glucose Tolerance Test
Abstract: The aim of this article was to define the metabolic phenotype of pregnant women with one abnormal value (OAV) during an oral glucose tolerance test (OGTT) and to test whether OAV could be considered metabolically comparable to gestational diabetes mellitus (GDM) or a specific entity between GDM and normal pregnancy.After 100-g 3-h OGTTs, 4,053 pregnant women were classified as having GDM, OAV, or normal glucose tolerance (NGT). Those with OAV were subdivided into three subgroups: fasting hyperglycemia (one abnormal value at fasting during an OGTT), 1-h hyperglycemia (one abnormal value at 1 h during an OGTT [1h-OAV]), or 2- or 3-h hyperglycemia (one abnormal value at 2 or 3 h during an OGTT). As derived from the OGTT, we measured insulin sensitivity (insulin sensitivity index [ISI] Matsuda) and insulin secretion (homeostasis model assessment for the estimation of beta-cell secretion [HOMA-B], first- and second-phase insulin secretion). The product of the first-phase index and the ISI was calculated to obtain the insulin secretion-sensitivity index (ISSI).GDM was diagnosed in 17.9% and OAV in 18.7% of pregnant women; women with GDM and OAV were older and had higher BMI and serum triglyceride levels than those with NGT (all P0.05). Women with NGT had the highest ISI followed by those with OAV (-21.7%) and GDM (-32.1%). HOMA-B results were comparable with those for OAV and GDM but significantly (P0.01) lower than those for NGT; first- and second-phase insulin secretion appeared progressively reduced from that in women with NGT to that in women with OAV and GDM (P0.01). ISSI was higher in women with NGT than in women with either OAV (-34%) or GDM (-51.7%) (P0.001). Among OAV subgroups, the 1h-OAV subgroup showed the lowest ISSI (P0.05).OAV and GDM are clinically indistinguishable, and both groups are different from women with NGT. Women with GDM and OAV showed impaired insulin secretion and insulin sensitivity, although these defects are more pronounced in women with GDM. Compared with other OAV subgroups, 1h-OAV could be considered a more severe condition.
Published: 2007

30. C-reactive protein and metabolic syndrome in women with previous gestational diabetes

Author: L Volpe, G. Di Cianni, Alessandra Ghio, Ilaria Cuccuru, G. Pellegrini, S. Del Prato, Roberto Miccoli, Luca Benzi, and Cristina Lencioni
Subjects: Adult, medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, Population, chemistry.chemical_compound, Endocrinology, Pregnancy, Reference Values, Diabetes mellitus, Internal medicine, Abdomen, Internal Medicine, medicine, Humans, Obesity, education, Homocysteine, Abdominal obesity, Triglycerides, Metabolic Syndrome, education.field_of_study, medicine.diagnostic_test, biology, business.industry, C-reactive protein, Fibrinogen, c-reactive protein, metabolic syndrome, gestational diabetes, medicine.disease, Gestational diabetes, Diabetes, Gestational, Parity, C-Reactive Protein, chemistry, Hyperglycemia, biology.protein, Uric acid, Female, medicine.symptom, Metabolic syndrome, Lipid profile, business, Lipoproteins, HDL
Abstract: Background This study evaluates the presence of metabolic syndrome (MS) and its association with C-reactive protein (CRP) and other cardiovascular (CV) risk factors, in a sample of women with and without previous Gestational Diabetes (pGDM). Methods One hundred and sixty-six women with pGDM and 98 women (controls) with uncomplicated pregnancy were studied 16 months after delivery. In all women, plasma glucose, insulin, lipid profile, serum uric acid, C-reactive protein, fibrinogen and homocysteine were measured. MS was defined according to NCEP ATPIII criteria. Results MS was identified in 15 pGDM women (9%) versus 1 control (1%) (p < 0.001). The more frequent metabolic traits were abdominal obesity (36% vs 17%) and low HDL-cholesterol (34% vs 17% in pGDM women and controls, respectively; all p < 0.01). HOMA-R, LDL-cholesterol, fibrinogen, serum uric acid and CRP resulted significantly higher in pGDM women with MS as compared to those without MS after adjustment for BMI. In women with no criteria for MS, only CRP levels were found to be higher in pGDM women compared to controls (p < 0.05). Seventeen percent of pGDM women with no criteria for MS had CRP levels ≥1 mg/L (all controls showed CRP levels
Published: 2006

31. Early impairment of beta-cell function and insulin sensitivity characterizes normotolerant Caucasian women with previous gestational diabetes

Author: Alessandra Ghio, Roberto Miccoli, Stefano Del Prato, Riccardo C. Bonadonna, Cristina Lencioni, L Volpe, Luca Benzi, Ilaria Cuccuru, Graziano Di Cianni, and Kyriazoula Chatzianagnostou
Subjects: Adult, Blood Glucose, medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Medicine (miscellaneous), beta cell function, White People, Impaired glucose tolerance, Insulin resistance, Pregnancy, Insulin-Secreting Cells, Internal medicine, insulin resistance, medicine, Humans, Insulin, Glucose tolerance test, Nutrition and Dietetics, medicine.diagnostic_test, business.industry, nutritional and metabolic diseases, Type 2 Diabetes Mellitus, Glucose Tolerance Test, medicine.disease, Impaired fasting glucose, Gestational diabetes, Diabetes, Gestational, Endocrinology, gestational diabetes, Female, Blood sugar regulation, Cardiology and Cardiovascular Medicine, business
Abstract: Women with previous gestational diabetes (pGDM) are at high risk of developing type 2 diabetes mellitus. The aim of this study was to evaluate insulin action and insulin secretion in women with pGDM.One hundred and fifty-three pGDM women and 45 with normal glucose tolerance during pregnancy (controls) were studied 1-3years after delivery. Insulin sensitivity (ISI) and beta-cell secretory capacity (beta-index) were derived from 75-g OGTT. Disposition Index was calculated as the product of beta-index and ISI. One hundred and twenty-two pGDM were normotolerant (NGT) and 31 had impaired glucose regulation (IGR) i.e. impaired glucose tolerance and/or impaired fasting glucose. NGT-pGDM, as compared to controls, had significant impairment in insulin action (ISI: 5.46+/-2.81 vs. 7.38+/-3.68, P0.01) and insulin secretion (beta-index: 4.68+/-1.01 vs. 5.24+/-0.82 pmol/min/m(2); P0.01). A further impairment was apparent in IGR-pGDM for beta-index (4.16+/-1.09; P0.05). The disposition index was reduced in NGT-pGDM as compared to controls (33.9%) and further reduced in IGR-pGDM (28.6%, vs. NGT-pGDM; ANOVA P0.001). In women of normal weight, ISI and beta-index were significantly (P0.01) impaired in NGT-pGDM compared to controls and further reduced in IGR-pGDM, although a more pronounced defect in insulin secretion was apparent in these women (beta-index: 4.02+/-0.9; P0.05).Normotolerant women with pGDM show both impairment in insulin secretion and action irrespective of body weight. A more pronounced defect in insulin secretion seems to characterize normal weight women while a more prominent defect in insulin action is found in overweight women.
Published: 2006

32. Maternal metabolic control and perinatal outcome in women with gestational diabetes mellitus treated with Lispro or Aspart insulin

Author: L Volpe, Luca Benzi, Stefano Del Prato, Graziano Di Cianni, Ilaria Cuccuru, Cristina Lencioni, and Alessandra Ghio
Subjects: Advanced and Specialized Nursing, medicine.medical_specialty, Pregnancy, endocrine system diseases, business.industry, Endocrinology, Diabetes and Metabolism, Insulin, medicine.medical_treatment, nutritional and metabolic diseases, medicine.disease, female genital diseases and pregnancy complications, Gestational diabetes, Insulin aspart, Postprandial, Endocrinology, Internal medicine, Diabetes mellitus, Internal Medicine, medicine, Regular insulin, Insulin lispro, business, medicine.drug
Abstract: Gestational diabetes mellitus (GDM) is associated with increased risk of maternal and neonatal morbidity with macrosomia being the most common neonatal complication (1). The risk of macrosomia and/or disproportionate fetal growth is closely related to 1-h postprandial glucose concentration (2). Therefore, the treatment of GDM should be aimed at normalizing maternal glycemia including the early postprandial response. Insulin therapy is needed whenever strict normoglycemia cannot be achieved by medical nutritional therapy alone (3). Because of their pharmacokinetic properties, short-acting insulin analogs (Insulin Aspart [ASP] and Insulin Lispro [LIS]) could be more effective in pregnancy than human regular insulin (HI) (4). Nevertheless, data …
Published: 2005

33. Maternal triglyceride levels and newborn weight in pregnant women with normal glucose tolerance

Author: Ilaria Cuccuru, L Volpe, Antonio Boldrini, Cristina Lencioni, G. Di Cianni, Roberto Miccoli, Alessandra Ghio, S. Del Prato, M. G. Giovannitti, G. Pellegrini, and Kyriazoula Chatzianagnostou
Subjects: Adult, Blood Glucose, medicine.medical_specialty, newborn weight, Endocrinology, Diabetes and Metabolism, Birth weight, Pregnancy, High-Risk, triglyceride levels, normal glucose tolerance, Body Mass Index, Impaired glucose tolerance, chemistry.chemical_compound, Endocrinology, Apolipoproteins E, Pregnancy, Diabetes mellitus, Internal medicine, Internal Medicine, medicine, Birth Weight, Humans, Triglycerides, Glucose tolerance test, Polymorphism, Genetic, medicine.diagnostic_test, Triglyceride, business.industry, Cholesterol, HDL, Infant, Newborn, Pregnancy Outcome, Cholesterol, LDL, Glucose Tolerance Test, Middle Aged, medicine.disease, Gestational diabetes, Diabetes, Gestational, chemistry, Gestation, Female, medicine.symptom, business, Weight gain
Abstract: To determine the predictive value of serum triglyceride levels (TG) for neonatal weight in pregnant women with positive diabetic screening but normal glucose tolerance.We enrolled 180 pregnant Caucasian women with positive diabetic screening. All women underwent a 3-h 100-g oral glucose tolerance test (OGTT) at 27th +/- 4 week of gestation. At the time of OGTT, we measured: fasting plasma glucose, fasting lipids profile and determined ApoE polymorphisms to evaluate the effects on lipid levels. In 83 women with normal glucose tolerance and at term delivery we evaluated the association between maternal serum TG, specific maternal parameters known to affect fetal growth and newborn weight.Based on OGTT, gestational diabetes mellitus (GDM) was diagnosed in 36 women (20%), impaired glucose tolerance (IGT) in 23 (13%), and normal glucose tolerance (NGT) in 121 (67%). Serum TG concentration was significantly higher in women with GDM (2.47 +/- 0.77 mmol/l) as compared with NGT (1.99 +/- 0.64 mmol/l) or IGT (1.98 +/- 0.81 mmol/l) (P0.01). ApoE3 allelic frequency was 86%, ApoE2 and ApoE4 were 5 and 9%, respectively. We found no clear-cut association between apoE genotype and serum TG concentration. Macrosomia and LGA newborns were more frequent in IGT than in GDM or NGT (P0.01). In the 83 women with positive diabetic screening but normal glucose tolerance who delivered at term, the incidence of LGA infants was significantly higher in those with TG levels higher than the 75th percentile (2.30 mmol/l) (21%) than in mothers who had normal TG levels (4.5%) (P0.05). Pre-pregnancy BMI (r(2) = 0.067), weight gain during pregnancy (r(2) = 0.062), fasting serum TG (r(2) = 0.09), and 2-h post-OGTT glucose levels (r(2) = 0.044) were all associated with neonatal body weight (all P0.05 or less). However, on a multiple regression analysis, only pre-pregnancy BMI (F-test = 7.26, P0.01), and fasting serum TG (F-test = 4.07, P0.01) were independently associated with birth weight.Pre-pregnancy BMI and fasting maternal serum TG determined in the last trimester of gestation were independently associated with neonatal birth weight in women with normal glucose tolerance, but positive screening test. TG levels measured in the third trimester of pregnancy are independent of the genetic polymorphism of ApoE.
Published: 2004

34. Improved insulin secretory function and reduced chemotactic properties after tissue culture of islets from type 1 diabetic patients

Author: Marco Bugliani, Marco Del Chiaro, Stefano Del Prato, Ugo Boggi, Lorella Marselli, Franco Mosca, Francesco Dotta, Roberto Lupi, S. Dionisi, Silvia Del Guerra, Cristina Lencioni, Umberto Di Mario, and Piero Marchetti
Subjects: Adult, Male, medicine.medical_specialty, Arginine, Adolescent, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Pyruvate Kinase, Glycerolphosphate Dehydrogenase, Glibenclamide, Tissue Culture Techniques, Islets of Langerhans, Endocrinology, Diabetes mellitus, Internal medicine, Fructose-Bisphosphate Aldolase, Glucokinase, Insulin Secretion, Internal Medicine, medicine, Humans, Insulin, RNA, Messenger, Child, Type 1 diabetes, geography, geography.geographical_feature_category, business.industry, Reverse Transcriptase Polymerase Chain Reaction, Pancreatic islets, Chemotaxis, medicine.disease, Islet, medicine.anatomical_structure, Diabetes Mellitus, Type 1, Glucose, Cytokines, Female, business, medicine.drug
Abstract: Background Type 1 diabetes results from the destruction of pancreatic beta-cell as a consequence of an autoimmune process. To date, information on the properties of islets isolated from type 1 diabetic patients is very scant. Methods Some immunological and functional properties of islets prepared from the pancreas of type 1 diabetic patients were studied shortly after the isolation and after a period of culture in euglycemic condition. Results Compared to control islets, freshly prepared type 1 diabetic islets released a significantly higher amount of cytokines (pg/mL) into the culture medium (TNF-α: 112.9 ± 5.6 vs 75.6 ± 24.4; INF-γ: 286.9 ± 26.9 vs 58.6 ± 6.2; IL-10: 41.8 ± 4.3 vs 10.1 ± 3.2; TGF-1 β: 294.0 ± 20.6 vs 45.1 ± 3.5); had a significantly higher chemotactic index (1.9 ± 0.2 vs 1.2 ± 0.1); showed reduced insulin release (% of insulin content) in response to glucose (2.8 ± 0.7 vs 5.3 ± 1.9), arginine (3.0 ± 0.6 vs 5.6 ± 1.0), and glibenclamide (2.9 ± 0.7 vs 5.4 ± 0.9); and exhibited decreased glucose oxidation capability and diminished mRNA expression of glucokinase, aldolase, pyruvate kinase, and mitochondrial glycerolphosphate dehydrogenase. Ten days after isolation, a normalization of cytokine release (TNF-α: 55.7 ± 2.3; INF-γ: 53.9 ± 4.3; IL-10: 8.6 ± 0.7; TGF-1 β: 60.7 ± 12.4) and chemotactic index (1.3 ± 0.2) were observed. Moreover, there was an improvement of insulin secretion (3.8 ± 0.3, 4.7 ± 0.6 and 3.5 ± 0.2 respectively in response to glucose, arginine, and glibenclamide) and glucose oxidation, and a partial recovery of the measured mRNA expressions. Conclusions These novel results, obtained with islets prepared from patients with type 1 diabetes, demonstrate that even after months after diabetes diagnosis, a period of culture of the islets in a more favorable environment has beneficial effects on the islet function. Copyright © 2004 John Wiley & Sons, Ltd.
Published: 2004

35. Role of kinin B1 and B2 receptors in the development of pilocarpine model of epilepsy

Author: Elaine Cristina Lencioni, João Bosco Pesquero, Gustavo Adolfo Argañaraz, José Antônio Silva, Michael Bader, Maria da Graça Naffah-Mazzacoratti, Sandra Regina Perosa, and Esper A. Cavalheiro
Subjects: Mossy fiber (hippocampus), Male, medicine.medical_specialty, Receptor, Bradykinin B2, Hippocampus, Hippocampal formation, Biology, Receptor, Bradykinin B1, Epilepsy, Mice, Internal medicine, medicine, Animals, Molecular Biology, Mice, Knockout, General Neuroscience, Dentate gyrus, Pilocarpine, Kinin, medicine.disease, Granule cell, Mice, Inbred C57BL, Disease Models, Animal, Endocrinology, medicine.anatomical_structure, Neurology (clinical), Developmental Biology, medicine.drug
Abstract: The tissue sclerosis found in epilepsy of limbic origin is characterized by shrunken gliotic hippocampus, granule cell loss in the dentate gyrus and extensive pyramidal cell loss in Ammon's horn. Evidence has indicated that sprouting of dentate granule cell axons into the inner molecular layer of the dentate gyrus is related to hyperexcitability. Trying to understand the role of kinin B1 and B2 receptors in the physiopathology of temporal lobe epilepsy (TLE), the present work was delineated to study the development of the epilepsy model induced by pilocarpine in B1 and B2 knockout mice (B1KO and B2KO, respectively). Behavior parameters, cell death and mossy fiber sprouting were analyzed. B1KO mice showed increased latency for the first seizure, associated to a decreased frequency of spontaneous seizures, when compared with wild-type mice. In addition, B1KO mice showed less cell death in all hippocampal formation associated to a reduced grade of mossy fiber sprouting. Furthermore, B2KO mice presented minor duration of the silent period and an increased frequency of spontaneous seizures, when compared with wild-type mice. B2KO and their control lineage showed similar pattern of cell death in the hippocampus, which was very intense when compared with saline-treated animals. The mossy fiber sprouting was also increased in B2KO mice, when compared to wild-type mice and saline-treated animals. Taken together, these data suggest a deleterious effect for kinin B1 receptor and a protective effect for kinin B2 receptor during the development of the temporal lobe epilepsy.
Published: 2004

36. 40(th) EASD Annual Meeting of the European Association for the Study of Diabetes : Munich, Germany, 5-9 September 2004

Author: S. Artigas, A V Dreval, Mark I. McCarthy, C Watson, Peter H. Bennett, M Quint, Y Ikeda, E Alpert, F Schiele, H Sekihara, Erik Gylfe, P Lowe, J Kuhlmann, Alain Golay, V Longo, Shahidul Alam Khan Akm., L G Mantovani, M Zawodniak-Szalapska, G Winkler, T Harrity, L Virág, U Johne, Kuo S-W., Linda C Tapsell, J Rodriguez, Michel Komajda, K Kankova, Carole A. Cull, M Sporna, E Estilles, U Ribel, M C Spruce, E Buzzigoli, T Prazak, J K McLaughlin, M K Lingohr, M Lim, F Calara, A Siebenhofer, G Meregalli, Roberto Anichini, A D Baron, R Kurashvili, P C Butler, G I Fantus, T. E. De Gooyer, Park Y-M., R. Walther, S Heinrich, Agnieszka Zawiejska, S Mukherjee, Nikolaos Papanas, G Wong, Ian D. Caterson, David M. Maahs, Shuichi Kaneko, Alexandra E. Butler, Francisco Javier Ampudia-Blasco, O N Kong, Attali J-R., C A Hedman, K Oshinyemi, Nicolle Müller, I C Cranston, N Okumus, M V Vlaiculescu, Balasubramanian Ravikumar, W W Cheatham, K Mukasa, K B Biswas, Annunziata Lapolla, Phil McEwan, G Mader, Gilles Chassot, Dragi Anevski, Werner A. Scherbaum, M Donath, C Hesselmann, R A Gandhi, David E. Moller, Ezio Bonifacio, C Garcia, V Ifandi, P Hornnes, Nieuwenhoven Fav., C Puech, S Pérez-Del-Pulgar, Kim S-R., G Hines, C Rubio Terrés, Michael Gaster, N. Hosszufalusi, A Scholze, Andrew A. Young, Stavros Liatis, F Hariri, S Tan, Paul Valensi, Allan E. Karlsen, J Kim, E. Moberg, J Kaiser, L Berman, G Nelson, A Altkrüger, P Kothare, D B Cook, S Doran, G. van Dijk, Shahnaz Shahinfar, Kim C-S., P Stahl, M Manousaki, S Sigrist, S K Lim, M. P. Stern, A Guberti, C Rezzani, J McKenney, Karl Thomaseth, Sofia Carlsson, M Julia, R Brillante, I Rubesova, T Darkow, E Matsumoto, Wendy M. Macfarlane, M Di Martino, G Bardini, Rossella Menghini, D Duhot, E Farcasiu, Annalisa Natalicchio, I Lindner, J Buvat, Christian L. Brand, Harry Dorchy, Iwona Pietrzak, Z T Luo, P Home, M Ekelund, Jesper Gromada, Kristine Færch, F Piarulli, H Kim, R Mentel, Zsuzsanna K. Zsengellér, Dullaart Rpf., Anton Luger, Thomas A. Pearson, V Manicardi, P Rösen, Feng Y-M., R Morganti, Lars Hansen, Demuth H-U., Haruo Kasai, A Shostak, Rudi Steffensen, G Taylor, Markolf Hanefeld, C Santini, E Hamaguchi, Roberto Miccoli, F Storms, M Cooper, Y Lee, Allison E. Aiello, P Smith, T Suehiro, K Treece, M Waluś, Timothy A Welborn, Simone Baltrusch, E Kontela, S Chai, J Crean, H Yokoyama, Johan G. Eriksson, Rafael Hernández Hernández, J Rodríguez-Saldaña, M P Tornero, G Formoso, D. Lovell, E Bingham, A Mylonakis, M Manteghetti, D Fedele, Antonio Martín-Duce, Ralph A. DeFronzo, D Salcedo, Kurt Højlund, Antonio Petrone, Sheu Whh., C Gutierrez, Flavia Pricci, S Kurita, Z G Abbas, M M Benedetti, Philippe A. Halban, Daniel J. Cox, O Ljungkvist, Justine Davies, J Palsgaard, Lars Sjöström, E Bosi, L Janin-Manificat, W. F. Kelly, M. Fernandez, E Colak, O V Mulyarchik, B Kronshage, F Lang, M Erfurth, Takashi Kadowaki, N Jendrike, U Walter, J Wishart, Y. Neye, D Kim, N Furuhashi, M Barsotti, D Florow, L Ke, L Borgquist, N C Jackson, Ffolliott M. Fisher, V Baskar, K Yoshioka, Bryan A. Wolf, G Chabrier, R Skoumal, Livio Luzi, H Kose, I Pharisien, B. Klein, H Winiarska, M C Johnson, L Griffiths, Nonna Kravchun, C Combe, Baptist Gallwitz, J Zdychova, L Skorda, Jorma Ilonen, W Gao, I N Steen, A Terrinoni, P D Ambery, W Kern, C M Kusminski, Cho M-H., Paolo Pozzilli, Louise G. Grunnet, E Schönle, David R Matthews, Robert W. Taylor, Y Cohen, Kim H-S., M P Eccles, N B Tutuncu, D McDowell, Richard M. Bergenstal, K Takamatsu, T Steiner, Jaan Palgi, Valdemar Grill, N Niculescu, G Federici, S Lehto, P. M. McKeigue, M Barone, Michael E. Trautmann, S Smirnov, J Mannion, M Eto, C Rousseau, M Conti, C S Ernest, Antonio Ceriello, D H Schweitzer, Jung E-D., Andreas Festa, Avijit Lahiri, A Shepelkevich, A Murro, A Kollmann, Jonathan R.S. Arch, R Landgraf, Son H-Y., I Engelsberger, E Agardh, S Rodríguez-Mulero, P J Kraml, K Lee, D. F. Du Toit, E Kim, G Fadini, Williams Ajk., Philip Home, M B Antcieferov, C Perlemoine, D Perrea, Song X-L., D Ruggieri, Krister Bokvist, Heidi Sørensen, Bilbao, G Yoshino, J P Taylor, Shen H-M., S M Furier, R Urquhart, J Wohlgelernter, Jianping Weng, T. Baba, Q Hong, C Silva, Castaigne J-P., M Felaco, X X Zhang, M Jaroň, Milla Rosengård-Bärlund, J G Papp, Toshio Miyata, Lervang H-H., Park M-K., I Kinalska, A Long, Oomen Phn., N Kogawa, Ippolita Patrizia Patera, S. Karadeniz, Dinesh Selvarajah, D S Chung, A Wensaas, Richard Imrich, M Recasens, J Ruxer, O Buchea, E Wilpart, S P Stepanenko, Le Ttd., H Ohgawara, Mariaconsuelo Valentini, A Mondok, M Peltonen, Marianne O. Larsen, K Chatzianagnostou, Agneta Ståhle, A L Ferrari, L Bordier, F Maingrette, A Matsuda, G Vukomanovic, Jakob D. Wikstrom, T Yamakita, E Gorostiaga, J Jin, B Gopalan, Heinz Drexel, S Hewitt, Rury R. Holman, C Dieterle, T L Ruchti, N Asatiani, M Sidira, A Iezzi, A J Sommerfield, D Châtenet, M L Olsen, R Bergemann, C Koehler, T L Kuraeva, B Balas, Christian Berne, E Santos-Mazo, G Smith, A Siejka, R Kožnarová, A Mattina, S Sheikh, A Adomeit, M Rasmussen, J. Fagerudd, N Busciantella Ricci, Nuria Vilarrasa, E Hammar, T L Thoms, L Aydın, Ron G. Rosenfeld, A Nikolajuk, R Gos, C L Morgan, H L Yu, D Dheelchand, S Ramrath, N Boudriga, Jerome I. Rotter, C Jahannault, W M Weston, Folke Lindgärde, M Hertlova, D Knight, A Monroy-Mayorga, E Pardini, A Chamson-Reig, B Franke, Janie McCluskey, Joseph Bryan, C Nikolopoulou, Christie M. Ballantyne, Fausto Santeusanio, L Pegoraro, M Lee, A Klimenko, S Jaiveer, K. Pettersson-Fernholm, Michael A. Nauck, A Ekbom-Schnell, G Deferrari, Riccardo Schiaffini, S. Pampanelli, Khan Aka., David Hopkins, Maija Wessman, M Kamarinos, Noh J-H., O Ebisui, K McCarroll, Jeppe Sturis, Peter Nowotny, N Gorbenko, Åke Sjöholm, David G. Maggs, A E Halseth, B Cresci, A A Ortiz-Gress, A Korakovouni, O Matejkova, C E Mogensen, C J Lin, Ramon Gomis, H Seaman, C Granier, Yang C-H., F Assah, O Sanchez, Fausto Machicao, Peter G. Morris, Alberto Ortiz, A Giardinelli, D Bracaglia, A Gonzalo, S Pavlatos, Andreas Lechner, F Canovic, L Sjolind, Allan Vaag, Birgitte Bruun Nielsen, David A. Ziegler, Vito Lampasona, R Gershoni-Baruch, A. Dei Cas, H Renz, E Mena, Matthew Waltham, Kim D-M., H Levanen, D D Mick, Valentina Alexandrovna Peterkova, E Meskhishvili, Sarah Nutland, R Bustani, John R. 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D. Mountjoy, Z Morrison, Elizabeth A. Davis, Franco Cavallo, C Corvaja, R Antuña, Craig John Currie, H Linnebjerg, He Y-L., A J Palmer, Mariola R. Chacón, H Malinska, M. Jones, R Lichnovská, K Mandes, Paolo Tessari, T Mokhort, A Laina, H. L. Y. Chan, I Schmidt, R Banks, Richard G. IJzerman, L Ksinantova, G Setti, H Vaudry, A Gallo, V Spallone, Chen J-W., Thomas Danne, A Chong, M Hallschmid, S Aczel, S Hulme, N Islam, M Hosoi, P M Ternan, P Di Bartolo, N Bishara, T Shibasaki, Martin A. Osterhoff, Im S-S., M Jecht, T Hamaguchi, S Mattera, K Ways, Elizabeth Northam, U Rajala, Reinhard W. Holl, L Yang, S Panaiotopoulos, K Horvath, R Kluge, Thora B. Bodvarsdottir, Y Dong, Irene Alemanno, C McDougall, Reimar W. Thomsen, M Campbell, W Rabl, John Öhrvik, Yuichiro Yamada, Paola Ungaro, W Benzer, Mike Sampson, Roberto Trevisan, R G Radu, Aas A-M., P E Lobo, Ricardo Scott, S M Son, Josephine M. Forbes, T A Hillier, K L Wyne, Louis L. Nguyen, J Farmer, M H Tan, Kwon H-S., J Yang, L Sandvik, Franco Folli, A K Jenum, M Nguyen, W Pratipanawatr, A L Frederiksen, Rebecca Smith, Lee H-J., A Schäfer, C Manuelli, G S Denver, T Vukovich, B Maceira, K Matsumoto, K. Chokkalingam, Nurcan Üçeyler, P Modi, Timothy M. Morgan, S Mertens, B M Singh, Michaela Riedl, K Iso, C Cucurullo, G. F. Bottazzo, M Calvani, K Hur, J Wetzels, Kazuhiro Takahashi, Y Aso, H Stammer, M G Masding, Fitsum Guebre-Egziabher, J L González-Sánchez, L Armstrong, Alberto Maran, Peter G.F. Swift, S S Popovic, J Starczynski, E Vitacolonna, Luigi Laviola, R W Gelling, Marina Cardellini, D Barilla, Rosa de Diego Martínez, W H Landschulz, Anne Mette Rosenfalck, R K Wong, Kevin E. Schneider, K Peros, Giuseppe Nanni, F Zhang, I Rákóczi, T Iburi, M Nakhjavani, X Q Zhang, S Tournis, Per Lav Madsen, Graham A. Hitman, A. Tura, K Laubner, N D Kostic, Lawrence M. Dolan, R. Sinha Roy, J A Wagner, J. Tuomilehto, J Hauptman, M Abdel-Ghany, D Lacombe, Toralph Ruge, Johannes A Maassen, Triantafyllos Didangelos, K Sasaki, I Argüelles, Klaus Levin, C Popow, Emanuel Christ, R Chetty, L Baillet-Blanco, Jo-Ann Salmon, T Mine, James L. Trevaskis, I Franke, J Gorski, E A Andrianova, A Dayan, A Caballero, Aleksandra Gilis-Januszewska, M Yasujima, Z Kasalová, C.D.A. Stehouwer, F. K. Gorus, G A Nichols, A Glowania, David P. Strachan, P Fredlund, N. F. da Silva, P Reboldi, M Sausbier, K H Groenier, G Stuccio, N Guttman, K R Ahmed, A D Ristic, T Kapellen, J Coutcher, Aldo V. Greco, Oswald Wagner, A Zagayko, Maria Alevizaki, B B Zhang, W F Ferris, Jenny Fredriksson, Lois Jovanovic, J Hänninen, R De Giglio, Kazuo Yagui, O Potterat, P Hamliton, R E Scranton, B Mankovsky, A Stylianou, B Fellström, Abdel-Wahab Yha., M Kitagawa, Katherine L. Baldock, F R Johnson, F Baigts, S D'Addato, F J Sanz, A Mistry, S D Wise, T Pratipanawatr, U R Fölsch, James R.C. Parkinson, Claudia Sommer, C Park, F E Griffiths, M L Martí, R Demirtunc, S Taniguchi, J Lundkvist, T Siegmund, Juan Sztajzel, C Dienesch, F Baumgartner, L Scalone, T M Mckolanis, K Otake, Ullrik Pedersen-Bjergaard, T M Vriesendorp, Michael B. Wheeler, Henry Schmitt, Peter Hovind, S Lange, Stephane Roze, L. Van Gaal, B Klaproth, Anthony E. Civitarese, D Eckland, A Dagar, D F Hopkins, Kari Stefansson, C Gonzalez-Yanes, B Meyboom-de Jong, D. J. Betteridge, K Buhling, M Crepaldi, Ana M. Wägner, L Renna, L Volpe, R McBride, V Corbo, E O Brennesvik, R P Hayes, R Abdollahnia, G Viviani, C F Liew, Francisco Pérez-Bravo, Jeffrey Baron, Brian M. Frier, H H Samira, D Szentendrei, K. J. Schjoedt, W K Waldhäusl, D Gniuli, D Zou, G Tschank, V Urbančič, A L Nolan, Albertini J-P., J Malcomson, M Larbig, C Cheyssac, K Aurich, C M Kesson, S Heller, Maija E. Miettinen, R F Luco, Adrian J. Cameron, Luigi Mattiello, Z. Metelko, X E Zhang, M Parramón, I. G. Obrosova, J Fruchart, M Ilic, Björn Eliasson, Gilles Chatellier, M A Martín, D M Kendall, Holger Luthman, V F Varillas, D Maccubbin, Jang S-A., Amalia Gastaldelli, E Salzsieder, P. de Mol, A Yoshida, H D Lindner, D Gostiljac, M Just, Pan C-Y., J M Fujitaki, G Eiermann, K Bergenheim, A D Frick, A Agacdiken, K Varytimiadis, K Cseh, D A Jackson, S Calderari, Dena G. Hernandez, H M Liebich, K Min, F. de Zegher, Bernd Kulzer, K Han, Ulrich A. Müller, D Marrero, H Hatakeyama, René Koopman, Doo H-K., Petr Wohl, P. Sharp, P Forder, Thor Aspelund, N Meneveau, R M Schmülling, R Aubert, Thom Sam., H Youshikawa, M Ankelo, D Bowden, I Kelly, Frédéric Fumeron, M Sartini, Robert S. Sherwin, L Varadhan, A Criscimanna, John Betteridge, V Jelic, M Bartnik, N Lemke, B Ursø, A Bertoldo, A M Owona, H Okochi, L Pérez-Tamajó, S L Monfre, Daniel Brandhorst, K T Legg, Andries J. Smit, Veronica Sancho, Masashi Hirai, C Klein, Paul J. Thornalley, A Chaidaroglou, K Miura, B Zinman, O M Dvoynishnikova, J Plank, Jan Bolinder, C Lush, B Rubi, R Pozzilli, M Bashir, S A Shtandel, F Mosca, A Naskalska, Josef Vcelak, U Sausbier, P Cavaiani, T U Baehring, Michele Solimena, P Formisano, M Rastaldi, Bernard Thorens, J Ruzzin, E Arbit, M. Hori, Torkel B. Brismar, E Soltes Rak, A Filo, P Heinke, Matthew P. Coghlan, M Masotti, I Perevozskaya, K Ahn, I Moules, K Van Dyck, I Goldstein, Z Mathe, G Z Zhao, S Fajardo, J Taylor, S Chrul, J C Pareja, D Hadjidakis, A J Scheen, N Siddiqua, D C Cavan, R Grella, Krabbe S, H J Rochlitz, A E Hinkkanen, W Wilpshaar, Richard Stevens, M Dreyer, S Hara, X Wang, Melania Manco, D Gillen, Magalie A. Ravier, Olli Simell, John C. Lawrence, Kohnert K-D., Agardh C-D., A Berghold, L Kristensen, Grant Sfa., N Gursoy, Leif Groop, N Freemantle, Anja Schweizer, L Pala, Legros J-J., C. Di Pietro, N. Yamamoto, J Magyar, B Nikolovski, H Ikeda, D Lee, Bruce A. 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Liu, H, Jeong, I, Chae, M, Choi, M, Yoo, H, Kim, C, Yun, M, Na, M, Kang, Y, Kong, O, Son, S, Kim, I, Tanaka, N, Hosoi, M, Matsuyama, Y, Fukumoto, M, Yamakita, T, Yoshioka, K, Ishii, T, Sato, T, Fujii, S, Aoki, T, Shibata, T, Mizutani, N, Suzuki, J, Fowelin, J, Samuelsson, P, Brandrup wogsen, G, Okumura, K, Tokmakova, A, Staroverova, D, Antcieferov, M, Shutichina, I, Kuntchevich, G, Vriesendorp, T, Morélis, Q, Legemate, D, Schaper, F, Mainas, E, Gkioulmpasanis, I, Panagiotou, I, Vassilikos, G, Skorda, L, Sidira, M, Christoforidou, M, Alaveras, A, Artikis, V, Evdemon, E, Lechleitner, M, Koch, T, Ebenbichler, C, Sturm, W, Moretti, L, Moruzzo, D, Boldrini, E, Pandolfo, C, Kameyama, M, Iwasa, R, Cho, M, Nam, J, Huh, K, Kaplar, M, Paragh, G, Erdei, A, Csongradi, E, Garai, I, Varga, J, Galuska, L, Udvardy, M, Higa, M, Kaneko, Y, Hiroi, N, Koziarska, D, Nowacki, P, Majkowska, L, Luzniak, P, Wojciechowska luźniak, A, Tushuizen, M, Nieuwland, R, Snoeck, D, Sturk, A, Diamant, M, Aguiar, L, Bahia, L, Villela, N, Laflor, C, Conde, C, Bottino, D, Dorigo, D, Bouskela, E, Pu, S, Luo, Z, Lam, K, Dan, Q, Xu, A, Shen, J, Cheng, K, Xu, J, Thamer, C, Stefan, N, Haap, M, Heller, E, Tschritter, O, De Prado, A, Ortiz, A, Ybarra, J, Gich, I, Pou, J, Ehren, M, Roggenland, D, Reinsen, B, Klein, H, Rittig, K, Stock, J, Kocher, B, Balletshofer, B, Shon, H, Chung, D, Nakatani, Y, Matsuhisa, M, Kaneto, H, Hatazaki, M, Yoshiuchi, K, Katakami, N, Kawamori, D, Ohtoshi, K, Sakamoto, K, Matsuoka, T, Ozawa, K, Ogawa, S, Hori, M, Yamasaki, Y, Zitouni, K, Harry, D, Nourooz zadeh, J, Earle, K, Olesen, P, Franco, L, Corvaja, C, Semplicini, A, Ceylan işık, A, Arı, N, Rösen, P, Lee, I, Park, K, Jung, E, Shin, D, Jo, S, Obuobie, K, Prakash, P, Hanna, F, Lazarus, J, Varadhan, L, Gurushankar, J, James, D, Sheikh, S, Gaede, P, Zou, D, Vilarrasa, N, Perez maraver, M, Mena, E, Perez, D, Setti, G, Buckingham, R, Urbančič, V, Stefanovska, A, Bernjak, A, Ažman juvan, K, Kocijančič, A, Glowania, A, Filters, T, Fosmark, D, Torjesen, P, Kilhovd, B, Berg, T, Sandvik, L, Hanssen, K, Mentink, C, Donchenko, G, Stepanenko, S, Maingrette, F, Deng, H, Lindenmair, A, Freudenthaler, A, Baumgartner parzer, S, Nizheradze, K, Khoruzhenko, A, Tronko, N, Sheu, W, Ou, H, Shen, H, Lin, T, Wu, H, Yang, C, Mogylnytska, L, Schmoelzer, I, Davies, J, Band, M, Struthers, A, Prázný, M, Škrha, J, Kasalová, Z, Neelotpol, S, Jahan, P, Kauschke, S, Harrop, C, Schäfer, A, Widder, J, Eigenthaler, M, Walter, U, Uchimura, I, Ikebukuro, M, Kaibara, M, Hirata, M, Helal, R, Pervin, F, Yang, X, Jansson, P, Nagaev, I, Jack, M, Carvalho, E, Sunnerhagen, K, Cam, M, Cushman, S, Smith, U, Creely, S, Farmer, J, Gustafson, B, Kusminski, C, Krusinova, E, Wohl, P, Klementova, M, Lanska, V, Mcdougall, C, Kelly, I, Abbas, Z, Lutale, J, Archibald, L, Karunajeewa, H, Stingemore, N, Stuccio, G, Mcgechie, D, Muller, L, Hak, E, Goudzwaard, W, Montorsi, F, Homering, M, Sprenger, K, Goldstein, I, Asnaghi, V, Ferrari, G, Rastaldi, M, Gabellini, D, Dell'Antonio, G, Maestroni, A, Ruggieri, D, Luzi, L, Piemonti, L, Zerbini, G, Anafaroglu, I, Tutuncu, N, Sultana, M, Siddiqua, N, Iwasaki, T, Nakajima, A, Yoneda, M, Mukasa, K, Tanaka, S, and Sekihara, H
Subjects: 0303 health sciences, medicine.medical_specialty, business.industry, EASD, Endocrinology, Diabetes and Metabolism, Human physiology, medicine.disease, 03 medical and health sciences, 0302 clinical medicine, Diabetes mellitus, Family medicine, Internal Medicine, Medicine, business, 030217 neurology & neurosurgery, 030304 developmental biology
Published: 2004

37. Intermediate metabolism in normal pregnancy and in gestational diabetes

Author: G. Di Cianni, S. Del Prato, L Volpe, Cristina Lencioni, and Roberto Miccoli
Subjects: medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, normal pregnancy, Physiology, Type 2 diabetes, Endocrinology, Pregnancy, Reference Values, Internal medicine, Diabetes mellitus, Internal Medicine, medicine, Glucose homeostasis, Homeostasis, Humans, Insulin, Fetus, business.industry, medicine.disease, Hormones, Gestational diabetes, Diabetes, Gestational, Glucose, intermediate metabolism, Female, gestational diabetes, Metabolic syndrome, business
Abstract: Complex though integrated hormonal and metabolic changes characterize pregnancy. In the face of progressive decline in insulin action, glucose homeostasis is maintained through a compensatory increase in insulin secretion. This switches energy production from carbohydrates to lipids, making glucose readily available to the fetus. This precise and entangled hormonal and metabolic condition can, however, be disrupted and diabetic hyperglycemia can develop (gestational diabetes). The increase in plasma glucose level is believed to confer significant risk of complications to both the mother and the fetus and the newborn. Moreover, exposition of fetal tissues to the diabetic maternal environment can translate into an increased risk for development of diabetes and/or the metabolic syndrome in the adult life. In women with previous gestational diabetes, the risk of developing type 2 diabetes is greatly enhanced, to the point that GDM represents an early stage in the natural history of type 2 diabetes. In these women, accurate follow-up and prevention strategies are needed to reduce the subsequent development of overt diabetes. This paper will review current knowledge on the modifications occurring in normal pregnancy, while outlining the mechanisms. In this paper, we will review the changes of intermediary metabolism occurring during pregnancy. In particular, we will outline the mechanisms responsible for gestational diabetes; the link between these alterations and associated maternal and neonatal morbidity will be examined.
Published: 2003

38. Prevalence and risk factors for gestational diabetes assessed by universal screening

Author: Roberto Miccoli, G Teti, Cristina Lencioni, Kyriazoula Chatzianagnostou, L Volpe, S. Del Prato, Graziano Di Cianni, Luca Benzi, Pietro Bottone, Ilaria Cuccuru, and Alessandra Ghio
Subjects: Adult, medicine.medical_specialty, endocrine system diseases, Endocrinology, Diabetes and Metabolism, Population, Overweight, Endocrinology, Pregnancy, Risk Factors, Diabetes mellitus, Prevalence, Internal Medicine, medicine, Humans, Mass Screening, Family history, Risk factor, education, Societies, Medical, Gynecology, education.field_of_study, Obstetrics, business.industry, Body Weight, nutritional and metabolic diseases, Retrospective cohort study, General Medicine, Glucose Tolerance Test, medicine.disease, Body Height, female genital diseases and pregnancy complications, Gestational diabetes, Diabetes, Gestational, Parity, Italy, Cohort, Female, medicine.symptom, gestational diabetes, business
Abstract: In order to evaluate the prevalence of gestational diabetes mellitus (GDM) and the presence of risk factors for GDM, we conducted a retrospective study of a cohort of Italian women. In addition, we compared universal versus selective screening to validate the ADA's recommendations in our population. From June 1st, 1995 to December 31st, 2001, universal screening for GDM was performed in 3950 women. The glucose challenge test (GCT) was positive (GCT+) in 1389 cases (35.2%). The 1-h glucose level after GCT enabled us to diagnose GDM directly in 24 pregnant women. Oral glucose tolerance test (OGTT) was performed in 1221 GCT+ women (144 cases with GCT+ dropped out) and GDM was diagnosed in 284 (23.2%) of them. OGTT was also performed in 391 randomly chosen, women from the GCT negative (GCT-) group. In this last group 25 (6.3%) women had GDM. Thus, the total number of subjects with GDM was 333 out of 3806 with a prevalence of 8.74% in the entire cohort. Assuming that the rate of GDM observed in the random sample of GCT- women is applicable to the whole group of 2561 GCT- women, then 161 GCT- patients could also have GDM. This will further increase the estimated prevalence for the whole cohort up to 12.3% (i.e. 469 out of 3806 pregnant women). There were 236 (5.6%) women with a low risk for GDM (normal weight, age less than 25 years and without a family history of diabetes). In this group we found 34 cases and five cases with positive screening test and GDM, respectively. Thus, if we excluded low risk women from the screening test, as suggested by ADA recommendations, only five women with GDM would have been missed. However, about 95% of our population were at medium or high risk for GDM and, therefore, would have been screened. The rate of GDM was significantly higher in women with a positive history of diabetes, increasing age, previous pregnancies, pre-pregnancy overweight and short stature. After logistic regression analysis, GDM diagnosis was significantly correlated with age (P
Published: 2003

39. Lymphokine release from human lymphomononuclear cells after co-culture with isolated pancreatic islets: effects of islet species, long-term culture, and monocyte-macrophage cell removal

Author: Silvia Del Guerra, Piero Marchetti, Renzo Navalesi, Rosa Giannarelli, Cristina Lencioni, Franco Mosca, C. Tellini, Roberto Lupi, Mario Carmellini, and Lorella Marselli
Subjects: medicine.medical_specialty, Time Factors, Immunology, Cell, Biology, Biochemistry, Monocytes, Islets of Langerhans, TNF production, Internal medicine, Gamma interferon, medicine, Immunology and Allergy, Monocytes macrophages, Animals, Humans, Molecular Biology, Cells, Cultured, geography, geography.geographical_feature_category, Interleukin-6, Tumor Necrosis Factor-alpha, Pancreatic islets, Macrophages, Lymphokine, Hematology, Islet, Molecular biology, Coculture Techniques, Interleukin-10, medicine.anatomical_structure, Endocrinology, Leukocytes, Mononuclear, Cytokines, Interleukin-2, Tumor necrosis factor alpha, Cattle, Interferons
Abstract: This study evaluated the release of Th 1 and Th 2 cytokines from human lymphomononuclear cells (LMC) in response to purified human (HI) or bovine (BI) islets, and the role of long-term (3–4 weeks) islet culture and removal of monocyte-macrophage cells. The results showed that HI and BI caused a similar increase of the release of gamma interferon (IFN), IL-2 and IL-6 from LMC, whereas BI had a more marked effect than HI on IL-10 release. Culturing the islets had possible positive effects (reduction of IFN and IL-2), but also potentially negative effects (increase of TNF). Removal of monocyte-macrophage cells determined a significant reduction of IL-6, IL-10 and TNF production in response to xeno-islets.
Published: 2000

40. Chemotactic activity of culture supernatants of free and encapsulated pancreatic rat islets towards peritoneal macrophages

Author: S. Tritschler, Laurence Kessler, E. Alexandre, Piero Marchetti, V. Karsten, Cristina Lencioni, A. Belcourt, R. Navalesi, Michel Pinget, and Ph. Poindron
Subjects: Male, endocrine system, medicine.medical_specialty, Hot Temperature, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Clinical Biochemistry, Biochemistry, Macrophage chemotaxis, Islets of Langerhans, Mice, Endocrinology, Internal medicine, Insulin Secretion, medicine, Animals, Insulin, Rats, Wistar, Cells, Cultured, geography, geography.geographical_feature_category, biology, Chemistry, Chemotaxis, Biochemistry (medical), General Medicine, Fibroblasts, Islet, Proteinase K, Molecular biology, Rats, Culture Media, Conditioned, biology.protein, Macrophages, Peritoneal, Syngenic, Pancreatic islet transplantation, Cell culture supernatant, Endopeptidase K
Abstract: Longterm efficiency of encapsulated pancreatic islet transplantation is limited by macrophagic reaction at the surface of biocompatible membrane. The aim of this work was to investigate the influence of soluble factors released by free and encapsulated islets on macrophage chemotaxis. The culture mediums conditioned for 6 days by free and encapsulated rat islets were incubated with peritoneal murine, rat allo and syngenic macrophages to study their migration. Culture supernatants of rat fibroblasts and acinar cells, glucose-stimulated free rat islets and supernatants of free rat islets treated by heat and proteinase K were also tested for their chemotactic activity. Islets encapsulation decreased the chemotactic activity of culture medium conditioned for 6 days by free rat islets on murine (1.66 +/- 0.20 vs. 3.10 +/- 0.23; p < 0.001, n = 5) and rat allogenic macrophages (1.63 +/- 0.21 vs. 4.70 +/- 0.36; p < 0.001, n = 9). There was no migration of rat macrophages towards syngenic islets. Fibroblasts exhibited a very strong chemotactic effect as compared to acinar cells. Insulin was not involved in macrophage migration. Proteinase K treatment of culture supernatant of free rat islets totally inhibited the chemotactic activity. After heating at 56 degrees C and 100 degrees C, this activity was reduced to 41 +/- 7% and 32 +/- 5% of the initial activity, respectively. In conclusion, pancreatic islet stimulated macrophage migration by release of immunological specific proteins partly retained by macroencapsulation.
Published: 1999

41. Preparation and long-term culture of isolated human pancreatic islets

Author: Piero Marchetti, Alberto Coppelli, M. Carmellini, Roberto Lupi, Cristina Lencioni, R. Navalesi, S Del Guerra, C. Tellini, Pellegrino Masiello, Rosa Giannarelli, Franco Mosca, and M Lorenzetti
Subjects: Transplantation, medicine.medical_specialty, Pancreatic islets, Cell Culture Techniques, Islets of Langerhans Transplantation, Biology, In vitro, Tissue culture, Islets of Langerhans, Endocrinology, medicine.anatomical_structure, Cell culture, Internal medicine, medicine, Humans, Surgery, Tissue Preservation
Published: 1998

42. Effects of monensin on insulin release from isolated human islets

Author: Roberto Lupi, M Lorenzetti, Cristina Lencioni, Piero Marchetti, Rosa Giannarelli, S Del Guerra, Franco Mosca, P Cecchetti, Mario Carmellini, R. Navalesi, Luca Benzi, and C. Tellini
Subjects: medicine.medical_specialty, medicine.medical_treatment, Biology, Endocrine secretion, Islets of Langerhans, chemistry.chemical_compound, Tissue culture, Internal medicine, medicine, Humans, Insulin, Monensin, Cells, Cultured, Transplantation, geography, geography.geographical_feature_category, Ionophores, Islet, In vitro, Endocrinology, chemistry, Surgery, Intracellular transport, Insulin metabolism
Published: 1998

43. Pancreatic glucagon damages isolated human islet function

Author: R. Navalesi, Piero Marchetti, Rosa Giannarelli, Franco Mosca, S Del Guerra, Alberto Coppelli, C. Tellini, Roberto Lupi, M. Carmellini, Lorella Marselli, Cristina Lencioni, and M Lorenzetti
Subjects: medicine.medical_specialty, medicine.medical_treatment, Islets of Langerhans Transplantation, Peptide hormone, Biology, Glucagon, Islets of Langerhans, Gastrointestinal Agents, Internal medicine, Diabetes mellitus, medicine, Humans, Pancreatic hormone, Cells, Cultured, Transplantation, geography, geography.geographical_feature_category, Insulin, Graft Survival, medicine.disease, Islet, Endocrinology, Diabetes Mellitus, Type 1, Pancreatectomy, Damages, Surgery
Published: 1998

44. Use of Insulin Glargine During the First Weeks of Pregnancy in Five Type 1 Diabetic Women

Author: Luca Benzi, Cristina Lencioni, Alessandra Ghio, Graziano Di Cianni, L Volpe, Stefano Del Prato, Ilaria Cuccuru, and Kyriazoula Chatzianagnostou
Subjects: Adult, medicine.medical_specialty, Pediatrics, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Pregnancy in Diabetics, Insulin Glargine, Nocturnal hypoglycemia, Pregnancy, Internal medicine, Diabetes mellitus, Internal Medicine, Humans, Hypoglycemic Agents, Insulin, Medicine, Significant risk, Glycated Hemoglobin, Advanced and Specialized Nursing, Plasma glucose, business.industry, Insulin glargine, Body Weight, Infant, Newborn, Delivery, Obstetric, medicine.disease, Insulin, Long-Acting, Endocrinology, Female, Animal studies, business, medicine.drug
Abstract: The long-acting analog glargine is a new insulin with 24-h persistence. This peculiar peakless action profile accounts for significant risk reduction for nocturnal hypoglycemia and a more stable daily plasma glucose profile (1,2). Only a few reports have described the use of insulin glargine during human pregnancy, so its use is not recommended at present. Animal studies have addressed the safety and efficacy of glargine during pregnancy, showing no direct effect on reproduction and embryo-fetal development (3 ) . Recently, Devlin et al. (4) and Holstein et al. (5) reported the use of insulin glargine in two type 1 diabetic women; both cases were free of any pregnancy complications and …
Published: 2005

45. Role of kinin B1 and B2 receptors in the development of pilocarpine model of epilepsy

Author: Adolfo Argañaraz, Gustavo, Regina Perosa, Sandra, Cristina Lencioni, Elaine, Bader, Michael, Abrão Cavalheiro, Esper, da Graça Naffah-Mazzacoratti, Maria, Bosco Pesquero, João, and Antônio Silva Jr., José
Subjects: *HIPPOCAMPUS (Brain), *KININS, *EPILEPSY, *SPASMS
Abstract: The tissue sclerosis found in epilepsy of limbic origin is characterized by shrunken gliotic hippocampus, granule cell loss in the dentate gyrus and extensive pyramidal cell loss in Ammon''s horn. Evidence has indicated that sprouting of dentate granule cell axons into the inner molecular layer of the dentate gyrus is related to hyperexcitability. Trying to understand the role of kinin B1 and B2 receptors in the physiopathology of temporal lobe epilepsy (TLE), the present work was delineated to study the development of the epilepsy model induced by pilocarpine in B1 and B2 knockout mice (B1KO and B2KO, respectively). Behavior parameters, cell death and mossy fiber sprouting were analyzed. B1KO mice showed increased latency for the first seizure, associated to a decreased frequency of spontaneous seizures, when compared with wild-type mice. In addition, B1KO mice showed less cell death in all hippocampal formation associated to a reduced grade of mossy fiber sprouting. Furthermore, B2KO mice presented minor duration of the silent period and an increased frequency of spontaneous seizures, when compared with wild-type mice. B2KO and their control lineage showed similar pattern of cell death in the hippocampus, which was very intense when compared with saline-treated animals. The mossy fiber sprouting was also increased in B2KO mice, when compared to wild-type mice and saline-treated animals. Taken together, these data suggest a deleterious effect for kinin B1 receptor and a protective effect for kinin B2 receptor during the development of the temporal lobe epilepsy. [Copyright &y& Elsevier]
Published: 2004
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46. Hepatotoxicidade do veneno botrópico (Bothrops jararaca)

Author: Luzia Cristina Lencioni Sampaio, Luis Fernando Jantzen Gaspar, Jéssica Maroneze Sziminski, Amanda Andersson Pereira, Eduarda Aléxia Nunes Louzada Dias Cavalcanti, Cleuza Kuhn, and Daiser Paulo de Almeida Sampaio Junior
Subjects: Veterinary medicine, SF600-1100
Abstract: Os acidentes ofídicos são comuns na rotina clínico veterinária, ocorrendo tanto em animais de companhia quanto animais de produção. São descritos em todas as regiões do território brasileiro, sendo responsável por quadros clínicos graves e ocasionalmente o óbito. As características e efeitos dos venenos da maioria dos ofídios são bem descritas na literatura, sendo a ação hepatotóxica relatada em serpentes do gênero crotálico. No presente trabalho descrevemos um quadro de fotodermatite resultante de acidente com uma jararaca (Bothrops jararaca) em um bovino adulto, raça Nelore, criado na zona rural do município de Abelardo Luz (SC). As lesões surgiram duas semanas após a picada, mostraram evolução generalizada e regressão demorada. Os exames complementares mostraram alterações das enzimas hepáticas alguns dias após o acidente ofídico e foram relacionados ao quadro dermatológico apresentado. O paciente permaneceu sob observação e tratamento por um período de 120 dias, apresentando melhora clínica significativa e regressão total das lesões.
Published: 2018
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