10 results on '"Cristiana Laudi"'
Search Results
2. Correction to: Evaluation of global and intragenic hypomethylation in colorectal adenomas improves patient stratification and colorectal cancer risk prediction
- Author
-
Carla Debernardi, Laura Libera, Enrico Berrino, Nora Sahnane, Anna Maria Chiaravalli, Cristiana Laudi, Mattia Berselli, Anna Sapino, Fausto Sessa, Tiziana Venesio, and Daniela Furlan
- Subjects
Genetics ,Correction ,Molecular Biology ,Genetics (clinical) ,Developmental Biology - Published
- 2021
3. Evaluation of global and intragenic hypomethylation in colorectal adenomas improves patient stratification and colorectal cancer risk prediction
- Author
-
Anna Maria Chiaravalli, Cristiana Laudi, Fausto Sessa, Nora Sahnane, Mattia Berselli, Carla Debernardi, Anna Sapino, Enrico Berrino, Laura Libera, Tiziana Venesio, and Daniela Furlan
- Subjects
Adenoma ,Male ,Oncology ,Neuroblastoma RAS viral oncogene homolog ,medicine.medical_specialty ,Colorectal cancer ,Colonoscopy ,Bisulfite pyrosequencing ,medicine.disease_cause ,Risk Assessment ,Cohort Studies ,L1-MET ,Predictive Value of Tests ,Internal medicine ,Biomarkers, Tumor ,Genetics ,Humans ,Medicine ,Genetic Predisposition to Disease ,Genetic Testing ,Molecular Biology ,Genetics (clinical) ,Aged ,medicine.diagnostic_test ,business.industry ,Research ,Colorectal adenomas ,CRC risk ,LINE-1 hypomethylation ,DNA Methylation ,Middle Aged ,medicine.disease ,Human genetics ,Italy ,Cohort ,Female ,KRAS ,Symptom Assessment ,Colorectal Neoplasms ,business ,Forecasting ,Developmental Biology ,DNA hypomethylation - Abstract
Background Aberrant DNA hypomethylation of the long interspersed nuclear elements (LINE-1 or L1) has been recognized as an early event of colorectal transformation. Simultaneous genetic and epigenetic analysis of colorectal adenomas may be an effective and rapid strategy to identify key biological features leading to accelerated colorectal tumorigenesis. In particular, global and/or intragenic LINE-1 hypomethylation of adenomas may represent a helpful tool for improving colorectal cancer (CRC) risk stratification of patients after surgical removal of polyps. To verify this hypothesis, we analyzed a cohort of 102 adenomas derived from 40 high-risk patients (who developed CRC in a post-polypectomy of at least one year) and 43 low-risk patients (who did not develop CRC in a post-polypectomy of at least 5 years) for their main pathological features, the presence of hotspot variants in driver oncogenes (KRAS, NRAS, BRAF and PIK3CA), global (LINE-1) and intragenic (L1-MET) methylation status. Results In addition to a significantly higher adenoma size and an older patients’ age, adenomas from high-risk patients were more hypomethylated than those from low-risk patients for both global and intragenic LINE-1 assays. DNA hypomethylation, measured by pyrosequencing, was independent from other parameters, including the presence of oncogenic hotspot variants detected by mass spectrometry. Combining LINE-1 and L1-MET analyses and profiling the samples according to the presence of at least one hypomethylated assay improved the discrimination between high and low risk lesions (p = 0.005). Remarkably, adenomas with at least one hypomethylated assay identified the patients with a significantly (p p = 0.02). Conclusions LINE-1 and L1-MET hypomethylation in colorectal adenomas are associated with a higher risk of developing CRC. DNA global and intragenic hypomethylation are independent markers that could be used in combination to successfully improve the stratification of patients who enter a colonoscopy surveillance program. Graphic abstract
- Published
- 2021
4. Interferon and amantadine in combination as initial treatment for chronic hepatitis C patients
- Author
-
Salvatore Cusumano, Laura Sidoli, Aldo Manca, Franco Chieppa, Marco Tabone, Benedetto Delmastro, Alberto Biglino, Franco Brunello, Giuseppe Cariti, R. Bonardi, Guido Calleri, Mario Rizzetto, Angelo Pera, Cristiana Laudi, Patrizia Della Monica, and Massimo Andreoni
- Subjects
medicine.medical_specialty ,Chemotherapy ,Cirrhosis ,Hepatology ,Combination therapy ,business.industry ,medicine.medical_treatment ,Amantadine ,medicine.disease ,Gastroenterology ,Group A ,Group B ,Surgery ,Clinical trial ,Interferon ,Internal medicine ,medicine ,business ,medicine.drug - Abstract
Background/Aims : To evaluate the efficacy and tolerance of amantadine in combination with interferon in the treatment of chronic hepatitis C. Methods : Multi-centre trial including 180 chronic hepatitis C patients without cirrhosis, randomly enrolled to receive interferon 6 MU every other day for 6 months followed by 3 MU for further 6 months (group A, 90 patients), or the same schedule plus amantadine 200 mg/day (group B, 90 patients). Primary end-point was a sustained virological and biochemical response, secondary end-points were on-treatment (third month) and end-of-treatment response rates. Results : The two groups had similar demographic, biochemical and virological characteristics. A sustained response after 6 months follow-up was observed in 17% of group A and 24% of group B patients ( P not significant), an end-of-treatment response was observed in 37% in group A and 47% in group B ( P not significant), an on-treatment response was observed in 46% in group A and 61% in group B patients ( P Conclusions : Adding amantadine to interferon did not improve the sustained treatment efficacy. However, the rate of early response at the third month of therapy was significantly higher in the combination therapy group.
- Published
- 2001
5. Genotype–phenotype relationship in inflammatory bowel disease
- Author
-
Marco Daperno, Rodolfo Rocca, Cristiana Laudi, Elena Ercole, Giuseppe Rocca, Raffaello Sostegni, Marco Astegiano, C. Rigazio, and Angelo Pera
- Subjects
business.industry ,Concordance ,Family aggregation ,Disease ,Bioinformatics ,medicine.disease ,Ulcerative colitis ,Inflammatory bowel disease ,digestive system diseases ,Chromosome 16 ,Genotype ,Immunology ,Internal Medicine ,medicine ,business ,Chromosome 12 - Abstract
Crohn’s disease (CD) and ulcerative colitis (UC) are chronic diseases of unknown etiology. Much effort has been made in the last years to clarify the pathogenesis of inflammatory bowel disease (IBD). Data are not conclusive at the moment, but the most important known risk factor for developing IBD is a positive familial history. Genetic analyses have shown a linkage between loci on several chromosomes and IBD (IBD1 gene on chromosome 16 for CD and on chromosome 12 for UC). The association of genotype to specific phenotypes of disease could be hypothesized by the concordance of clinical characteristics in familial IBD, by the association of specific HLA haplotypes to clinically different groups of patients, and by different responses to treatment related to different polymorphisms of other chromosome 6 genes. The clinical heterogeneity of IBD has led to classifications of patients with Crohn’s disease based upon clinical features (e.g. Rome and Vienna classifications) that allow the identification of subgroups of patients with similar clinical behavior. The possible drawbacks of these classifications are the lack of validation of intra–interobserver concordance, the absence of prospective evaluations, and stratification into too many subgroups. Furthermore, in our experience, clinical presentation (surgical or medical) seems to have a good correlation with prognosis, is easy identifiable, and can be applied at the time of diagnosis. In UC, extension of disease and clinical behavior correlate with prognosis. For these reasons, studies correlating genotype to phenotype should be performed to improve our knowledge of the diseases and possibly to stratify patients into different subgroups for more personalized treatments, in clinical trials and for research purposes.
- Published
- 2000
6. Abdominal bowel ultrasound can predict the risk of surgery in Crohn's disease: proposal of an ultrasonographic score
- Author
-
Cristiana Laudi, Alessandro Lavagna, Marco Daperno, Luca Viganò, Franco Bertolino, Rodolfo Rocca, Angelo Pera, Elena Ercole, Raffaello Sostegni, C. Rigazio, and L. Crocellà
- Subjects
Adult ,Male ,medicine.medical_specialty ,Gastroenterology ,Risk Assessment ,Sensitivity and Specificity ,Severity of Illness Index ,Crohn Disease ,Predictive Value of Tests ,Recurrence ,Internal medicine ,Severity of illness ,medicine ,Odds Ratio ,Humans ,Risk factor ,Colectomy ,Probability ,Crohn's disease ,Analysis of Variance ,business.industry ,Ultrasound ,Case-control study ,Ultrasonography, Doppler ,Odds ratio ,medicine.disease ,Immunohistochemistry ,Surgery ,Intestines ,medicine.anatomical_structure ,Logistic Models ,Predictive value of tests ,Area Under Curve ,Case-Control Studies ,Disease Progression ,Abdomen ,Female ,business ,Follow-Up Studies - Abstract
Abdominal bowel ultrasound (US) is widely used in the management of Crohn's disease (CD). The aim of this study was to evaluate the prognostic role of bowel-wall US morphology on the short-term risk of surgery.The 147 CD patients recruited in a case-control study comprised 49 cases operated on within 30 days after US examination and 98 matched non-operated controls. Clinical and US characteristics were analysed. Bowel-wall thickness was recorded, bowel-wall patterns were grouped into five types, but for final analysis they were grouped as "preserved" or "disrupted stratification".Wall thickness and US patterns were significantly different between cases and controls (p0.0001). A wall thickness4.5 mm was observed in 45/49 cases and 47/98 controls (OR = 12.21), while "disrupted stratification" was observed in 34/49 cases and 12/98 controls (OR = 16.24). Among the clinical and US characteristics recorded, only 4 US variables were independently associated with surgery (pattern, thickness, presence of fistulae/abscesses and stenoses) and considered for the US score=(2.5*US pattern)+(1.5*Bowel thickness)+(3*Presence of fistulae/abscesses)+(1.5*Presence of stenoses). Based on this score, up to 84% of patients were correctly classified according to actual status (operated/non-operated).Although it needs further prospective validation, the score we propose seems to be a reliable prognostic marker for the short-term risk of surgery in CD. In particular, the score points out those patients with an impending risk of surgery who need careful and frequent control in order to decide on the right time for surgery.
- Published
- 2009
7. Fecal pancreatic elastase 1 in the work up of patients with chronic diarrhea
- Author
-
Patrizia Della Monica, G. Masoero, Caterina Zaffino, Cristiana Laudi, Rodolfo Rocca, Lucrezia Gallo, L. Lombardo, and Angelo Pera
- Subjects
Adult ,Diarrhea ,Male ,medicine.medical_specialty ,Pancreatic disease ,Adolescent ,Gastroenterology ,Inflammatory bowel disease ,Diagnosis, Differential ,Feces ,Endocrinology ,Chronic diarrhea ,Crohn Disease ,Internal medicine ,Medicine ,Humans ,Pancreatic elastase ,Aged ,Pancreatic Elastase ,business.industry ,Clinical Enzyme Tests ,Middle Aged ,medicine.disease ,Inflammatory Bowel Diseases ,Work-up ,Oncology ,Chronic Disease ,Female ,medicine.symptom ,Differential diagnosis ,business - Abstract
Quantitative determination of pancreatic elastase-1 (E1) in stools has been proposed as a novel, noninvasive test of pancreatic function. The aim of the study was to verify its role in the differential diagnosis of chronic diarrhea.E1 was measured in spot stool samples of 50 patients with pancreatic disease (PD), 62 with inflammatory bowel disease (IBD), 45 with chronic diarrhea (CD), 34 with other gastroduodenal and liver disease (gastrointestinal; GI), and in 18 normal controls, by a commercial kit (Schebo-Tech., Wettemburg, Germany).In PD, patients with severe damage and diarrhea displayed E1 levels below 100 microg/g; normal values were found in mild-moderate disease. Abnormal values were detected in 4 CD and in 14 IBD patients, either in the presence of severe protein malnutrition or in patients with previous ileo-anal pouch anastomosis and pouchitis. In nine cases, values reverted to normal after adequate treatment. Diagnostic accuracy of E1 in discriminating diarrhea of pancreatic and nonpancreatic origin was: SS, 97%; SP, 84%; VP+, 66%; VP-, 100%.1) The finding of a normal E1 value rules out a malabsorption of pancreatic origin. 2) in CD and IBD, decreased E1 might be owing to bacterial elastase degradation (pouchitis) or transient defective pancreatic enzyme secretion.
- Published
- 2001
8. Adjuvant iodine-131-labeled lipiodol for prevention of intrahepatic recurrence of hepatocellular carcinoma: Which is the best treatment schedule?
- Author
-
Cristiana Laudi, Luca Viganò, Alessandro Ferrero, Marco Tabone, Lorenzo Capussotti, and R.E. Pellerito
- Subjects
Oncology ,medicine.medical_specialty ,Hepatology ,business.industry ,medicine.medical_treatment ,chemistry.chemical_element ,medicine.disease ,Iodine ,Text mining ,chemistry ,Hepatocellular carcinoma ,Treatment Schedule ,Internal medicine ,medicine ,Lipiodol ,business ,Adjuvant ,medicine.drug - Published
- 2005
9. The diagnostic and therapeutic role of endoscopic retrograde cholangiopancreatography (ERCP) in biliary and pancreatic disorders in children
- Author
-
Marco Daperno, Rodolfo Rocca, Franco Coppola, Patrizia Della Monica, Luigi Calvo, Masoero Guya, F. Castellino, Cristiana Laudi, Cristiana Barbera, Raffaello Sostegni, and Angelo Pera
- Subjects
medicine.medical_specialty ,Endoscopic retrograde cholangiopancreatography ,Hepatology ,medicine.diagnostic_test ,business.industry ,General surgery ,Gastroenterology ,medicine ,Radiology ,business - Published
- 2001
10. A simple diagnostic score for bilio-pancreatic cancer
- Author
-
Elena Ercole, G. Masoero, Giuseppe Rocca, Marco Daperno, Paola Secreto, Angelo Pera, Franco Coppola, Raffaello Sostegni, Patrizia Della Monica, Cristiana Laudi, and Rodolfo Rocca
- Subjects
Oncology ,medicine.medical_specialty ,Hepatology ,business.industry ,Simple (abstract algebra) ,Pancreatic cancer ,Internal medicine ,Gastroenterology ,medicine ,medicine.disease ,business - Published
- 2000
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.