1. Biological and molecular characterization of a recombinant isolate of Watermelon Mosaic Virus associated with a watermelon necrotic disease in Italy
- Author
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Fabrizio Cillo, M. M. Finetti-Sialer, Tiziana Mascia, Crisostomo Vovlas, and Donato Gallitelli
- Subjects
Whole genome sequencing ,Cloning ,food.ingredient ,Phylogenetic analysis ,biology ,Melon ,food and beverages ,Plant Science ,Horticulture ,biology.organism_classification ,Genome ,Virology ,Virus ,Watermelon ,food ,WMV ,5?UTR/P1/HC-Pro region ,Genome sequence ,Watermelon mosaic virus ,Agronomy and Crop Science ,Cucumis ,Cucurbita maxima - Abstract
The biological and molecular characterization is reported of a Watermelon mosaic virus isolate, denoted WMV-Le, associated with a necrotic phenotype of watermelon plants grown in the Provinces of Lecce and Taranto (Apulia, southern Italy). The fully sequenced WMV-Le genome consists of 10,045 nucleotides and is 99.1% similar to that of WMV-C05-270, a French isolate from melon of the WMV molecular group 3. Using recombination detection program RDP3, putative recombination breakpoints were identified close to nucleotide positions 42 to 1892, covering the 5′UTR/P1/HC-Pro region. The event represents the insertion of a sequence fragment of an isolate similar to WMV-FBR04-37 in the background of an isolate similar to WMV-FMF00-LL1. The field symptomatology was reproduced in watermelon plants grown in an experimental greenhouse but the virus induced severe symptoms also in Cucumis sativus, C. melo, Cucurbita maxima and C. pepo .
- Published
- 2012
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