8 results on '"Crespo, Roberto Fernandez"'
Search Results
2. Implementing behavioural science informed letter interventions to increase COVID-19 vaccination uptake in uncontactable London residents: a difference-in-difference study in London, UK
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Huf, Sarah W, primary, Woldmann, Lena, additional, Crespo, Roberto Fernandez, additional, Grailey, Kate, additional, Hassanpourfard, Bahram, additional, Chisambi, Matthew, additional, Black, Kirstie, additional, Nguyen, Joe, additional, Klaber, Bob, additional, and Darzi, Ara, additional
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- 2022
- Full Text
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3. International attitudes on COVID-19 vaccination: repeat national cross-sectional surveys across 15 countries
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Crespo, Roberto Fernandez, primary, Shafat, Manar, additional, Melas-Kyriazi, Natalie, additional, Gould, Lisa, additional, Jones, Sarah, additional, Neves, Ana Luisa, additional, Leis, Melanie S, additional, Maheswaran, Hendramoorthy, additional, and Darzi, Ara, additional
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- 2021
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4. Evidence of illegitimate recombination between two Pasteurellaceae plasmids resulting in a novel multi-resistance replicon, pM3362MDR, in Actinobacillus pleuropneumoniae
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Silva, Giarlã Cunha da, Li, Yinghui, Li, Yanwen, Rossi, Ciro C., Crespo, Roberto Fernandez, Williamson, Susanna M., Langford, Paul R., Bazzolli, Denise Mara Soares, Bossé, Janine T., Biotechnology and Biological Sciences Research Council (BBSRC), BBSRC, and NC3Rs (National Centre for the Replacement, Refinement and Reduction of Animals in Research)
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plasmids ,Science & Technology ,GENES ,IDENTIFICATION ,SEQUENCES ,HAEMOPHILUS-PARASUIS ,Respiratory tract ,Tetracycline ,Antimicrobial resistance ,respiratory tract ,RELAXASES ,Microbiology ,MULTOCIDA ,ANTIMICROBIAL-RESISTANCE ,CONJUGATIVE ELEMENT ICE ,TRANSFER ORIGIN ,PIGS ,antimicrobial resistance ,Pasteurellaceae ,Life Sciences & Biomedicine ,Plasmids ,tetracycline - Abstract
Evidence of plasmids carrying the tetracycline resistance gene, tet(B), was found in the previously reported whole genome sequences of 14 United Kingdom, and 4 Brazilian, isolates of Actinobacillus pleuropneumoniae. Isolation and sequencing of selected plasmids, combined with comparative sequence analysis, indicated that the four Brazilian isolates all harbor plasmids that are nearly identical to pB1001, a plasmid previously found in Pasteurella multocida isolates from Spain. Of the United Kingdom isolates, 13/14 harbor plasmids that are (almost) identical to pTetHS016 from Haemophilus parasuis. The remaining United Kingdom isolate, MIDG3362, harbors a 12666 bp plasmid that shares extensive regions of similarity with pOV from P. multocida (which carries bla ROB−1 , sul2, and strAB genes), as well as with pTetHS016. The newly identified multi-resistance plasmid, pM3362MDR, appears to have arisen through illegitimate recombination of pTetHS016 into the stop codon of the truncated strB gene in a pOV-like plasmid. All of the tet(B)-carrying plasmids studied were capable of replicating in Escherichia coli, and predicted origins of replication were identified. A putative origin of transfer (oriT) sequence with similar secondary structure and a nic-site almost identical to that of RP4 was also identified in these plasmids, however, attempts to mobilize them from an RP4-encoding E. coli donor strain were not successful, indicating that specific conjugation machinery may be required.
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- 2018
5. Whole Genome Sequencing for Surveillance of Antimicrobial Resistance in Actinobacillus pleuropneumoniae
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Bossé, Janine T., Li, Yanwen, Rogers, Jon, Crespo, Roberto Fernandez, Li, Yinghui, Chaudhuri, Roy R., Holden, Matthew T. G., Maskell, Duncan J., Tucker, Alexander W., Wren, Brendan W., Rycroft, Andrew N., Langford, Paul R., BRaDP1T Consortium, University of St Andrews. School of Medicine, University of St Andrews. Infection Group, University of St Andrews. Infection and Global Health Division, University of St Andrews. Biomedical Sciences Research Complex, Maskell, Duncan [0000-0002-5065-653X], Tucker, Alexander [0000-0003-0062-0843], Apollo - University of Cambridge Repository, Biotechnology and Biological Sciences Research Council (BBSRC), and Pfizer Limited (UK)
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0301 basic medicine ,PREDICTION ,QH301 Biology ,MANNHEIMIA-HAEMOLYTICA ,Respiratory tract ,SUSCEPTIBILITY ,Agar dilution ,BACTERIAL PATHOGENS ,chemistry.chemical_compound ,Ampicillin ,CONJUGATIVE ELEMENT ICE ,animal infections ,PIGS ,Tilmicosin ,Original Research ,biology ,HAEMOPHILUS-PARASUIS ,Animal infections ,Genomics ,QR Microbiology ,Antimicrobial ,Integrative conjugative elements ,PASTEURELLA-MULTOCIDA ,integrative conjugative elements ,Life Sciences & Biomedicine ,medicine.drug ,Plasmids ,Microbiology (medical) ,plasmids ,Tetracycline ,030106 microbiology ,Tylosin ,Microbiology ,03 medical and health sciences ,QH301 ,Enrofloxacin ,medicine ,genomics ,Actinobacillus pleuropneumoniae ,Science & Technology ,IDENTIFICATION ,DAS ,biology.organism_classification ,respiratory tract ,QR ,Antimicrobial resistance genes ,030104 developmental biology ,chemistry ,Pasteurellaceae ,antimicrobial resistance genes - Abstract
This work was supported by a Longer and Larger (LoLa) grant from the Biotechnology and Biological Sciences Research Council (BBSRC grant numbers BB/G020744/1, BB/G019177/1, BB/G019274/1, and BB/G018553/1), the UK Department for Environment, Food and Rural Affairs, and Zoetis (formerly Pfizer Animal Health) awarded to the Bacterial Respiratory Diseases of Pigs-1 Technology (BRaDP1T) consortium. MH was supported by the Wellcome Trust (grant number 098051). JR was funded from the former AHVLA’s Research and Development Internal Investment Fund (grant number RD0030c). The aim of this study was to evaluate the correlation between antimicrobial resistance (AMR) profiles of 96 clinical isolates of Actinobacillus pleuropneumoniae, an important porcine respiratory pathogen, and the identification of AMR genes in whole genome sequence (wgs) data. Susceptibility of the isolates to nine antimicrobial agents (ampicillin, enrofloxacin, erythromycin, florfenicol, sulfisoxazole, tetracycline, tilmicosin, trimethoprim, and tylosin) was determined by agar dilution susceptibility test. Except for the macrolides tested, elevated MICs were highly correlated to the presence of AMR genes identified in wgs data using ResFinder or BLASTn. Of the isolates tested, 57% were resistant to tetracycline [MIC ≥ 4 mg/L; 94.8% with either tet(B) or tet(H)]; 48% to sulfisoxazole (MIC ≥ 256 mg/L or DD = 6; 100% with sul2), 20% to ampicillin (MIC ≥ 4 mg/L; 100% with blaROB-1), 17% to trimethoprim (MIC ≥ 32 mg/L; 100% with dfrA14), and 6% to enrofloxacin (MIC ≥ 0.25 mg/L; 100% with GyrAS83F). Only 33% of the isolates did not have detectable AMR genes, and were sensitive by MICs for the antimicrobial agents tested. Although 23 isolates had MIC ≥ 32 mg/L for tylosin, all isolates had MIC ≥ 32 mg/L for tylosin, all isolates had MIC ≤ 16 mg/L for both erythromycin and tilmicosin, and no macrolide resistance genes or known point mutations were detected. Other than the GyrAS83F mutation, the AMR genes detected were mapped to potential plasmids. In addition to presence on plasmid(s), the tet(B) gene was also found chromosomally either as part of a 56 kb integrative conjugative element (ICEApl1) in 21, or as part of a Tn7 insertion in 15 isolates. Our results indicate that, with the exception of macrolides, wgs data can be used to accurately predict resistance of A. pleuropneumoniae to the tested antimicrobial agents and provides added value for routine surveillance. Publisher PDF
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- 2017
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6. ICEApl1, an Integrative Conjugative Element Related to ICEHin1056, Identified in the Pig Pathogen Actinobacillus pleuropneumoniae
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Bosse, Janine T., Li, Yanwen, Crespo, Roberto Fernandez, Chaudhuri, Roy R., Rogers, Jon, Holden, Matthew T. G., Maskell, Duncan J., Tucker, Alexander W., Wren, Brendan W., Rycroft, Andrew N., Langford, Paul R., BRaDP1T Consortium, University of St Andrews. School of Medicine, University of St Andrews. Infection Group, and University of St Andrews. Biomedical Sciences Research Complex
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Antibiotic resistance ,Conjugation ,NDAS ,Respiratory tract ,Animal infections ,QR Microbiology ,Pasteurellaceae ,R Medicine ,QR - Abstract
This work was supported by a Longer and Larger (LoLa) grant from the Biotechnology and Biological Sciences Research Council (BBSRC grant numbers BB/G020744/1, BB/G019177/1, BB/G019274/1, and BB/G018553/1), the UK Department for Environment, Food and Rural Affairs, and Zoetis (formerly Pfizer Animal Health) awarded to the Bacterial Respiratory Diseases of Pigs-1 Technology (BRaDP1T) Consortium. MTGH was supported by the Wellcome Trust (grant number 098051). JR was funded from the former AHVLA’s Research and Development Internal Investment Fund (grant number RD0030c). ICEApl1 was identified in the whole genome sequence of MIDG2331, a tetracycline resistant (MIC = 8 mg/L) serovar 8 clinical isolate of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. PCR amplification of virB4, one of the core genes involved in conjugation, was used to identify other A. pleuropneumoniae isolates potentially carrying ICEApl1. MICs for tetracycline were determined for virB4 positive isolates, and shotgun whole genome sequence analysis was used to confirm presence of the complete ICEApl1. The sequence of ICEApl1 is 56083 bp long and contains 67 genes including a Tn10 element encoding tetracycline resistance. Comparative sequence analysis was performed with similar integrative conjugative elements (ICEs) found in other members of the Pasteurellaceae. ICEApl1 is most similar to the 59393 bp ICEHin1056, from Haemophilus influenzae strain 1056. Although initially identified only in serovar 8 isolates of A. pleuropneumoniae (31 from the UK and 1 from Cyprus), conjugal transfer of ICEApl1 to representative isolates of other serovars was confirmed. All isolates carrying ICEApl1 had a MIC for tetracycline of 8 mg/L. This is, to our knowledge, the first description of an ICE in A. pleuropneumoniae, and the first report of a member of the ICEHin1056 subfamily in a non-human pathogen. ICEApl1 confers resistance to tetracycline, currently one of the more commonly used antibiotics for treatment and control of porcine pleuropneumonia. Publisher PDF
- Published
- 2016
7. Complete genome sequence of MIDG2331, a genetically tractable serovar 8 clinical isolate of Actinobacillus pleuropneumoniae
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Crespo, Roberto Fernandez, Coupland, Paul, Bossé, Janine T., Chaudhuri, Roy R., Bazzolli, Denise M., Leanse, Leon G., Holden, Matthew T. G., Maskell, Duncan J., Tucker, Alexander W., Wren, Brendan W., Rycroft, Andrew N., and Langford, Paul R.
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Actinobacillus pleuropneumoniae ,MIDG2331 - Abstract
We report here the complete annotated genome sequence of a clinical serovar 8 isolate Actinobacillus pleuropneumoniae MIDG2331. Unlike the serovar 8 reference strain 405, MIDG2331 is amenable to genetic manipulation via natural transformation as well as conjugation, making it ideal for studies of gene function.
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- 2016
8. Identification of dfrA14 in two distinct plasmids conferring trimethoprim resistance in Actinobacillus pleuropneumoniae.
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Bossé, Janine T., Yanwen Li, Walker, Stephanie, Atherton, Tom, Crespo, Roberto Fernandez, Williamson, Susanna M., Rogers, Jon, Chaudhuri, Roy R., Weinert, Lucy A., Olusegun Oshota, Holden, Matt T. G., Maskell, Duncan J., Tucker, Alexander W., Wren, Brendan W., Rycroft, Andrew N., and Langford, Paul R.
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DRUG resistance in bacteria ,RESPIRATORY infections ,ACTINOBACILLUS pleuropneumoniae ,TRIMETHOPRIM ,ANIMAL models in research - Abstract
Objectives: The objective of this study was to determine the distribution and genetic basis of trimethoprim resistance in Actinobacillus pleuropneumoniae isolates from pigs in England. Methods: Clinical isolates collected between 1998 and 2011 were tested for resistance to trimethoprim and sulphonamide. The genetic basis of trimethoprim resistance was determined by shotgun WGS analysis and the subsequent isolation and sequencing of plasmids. Results: A total of 16 (out of 106) A. pleuropneumoniae isolateswere resistant to both trimethoprim (MIC>32 mg/L) and sulfisoxazole (MIC ≥256 mg/L), and a further 32 were resistant only to sulfisoxazole (MIC ≥256 mg/L). Genome sequence data for the trimethoprim-resistant isolates revealed the presence of the dfrA14 dihydrofolate reductase gene. The distribution of plasmid sequences in multiple contigs suggested the presence of two distinct dfrA14-containing plasmids in different isolates, which was confirmed by plasmid isolation and sequencing. Both plasmids encoded mobilization genes, the sulphonamide resistance gene sul2, aswell as dfrA14 inserted into strA, a streptomycin- resistance-associated gene, although the gene order differed between the two plasmids. One of the plasmids further encoded the strB streptomycin-resistance-associated gene. Conclusions: This is the first description of mobilizable plasmids conferring trimethoprim resistance in A. pleuropneumoniae and, to our knowledge, the first report of dfrA14 in any member of the Pasteurellaceae. The identification of dfrA14 conferring trimethoprim resistance in A. pleuropneumoniae isolates will facilitate PCR screens for resistance to this important antimicrobial. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
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