68 results on '"Covelli C"'
Search Results
2. Impact of Covid-19 pandemic on children with special needs requiring general anaesthesia for the treatment of dental disease: the experience of the Brescia Children’s Hospital, Lombardy, Italy
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Tewfik, K., primary, Peta, C., additional, De Giuli, M. C., additional, Rossini, M., additional, Giampaoli, G., additional, Covelli, C., additional, and Burlini, D., additional
- Published
- 2022
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3. A Broad-crested Weir Boundary Condition in Finite Volume Shallow-water Numerical Models
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Cozzolino, L., Morte, R. Della, Cimorelli, L., Covelli, C., and Pianese, D.
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- 2014
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4. Autoimmune biliary diseases: Primary biliary cholangitis and primary sclerosing cholangitis
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Sarcognato, S, Sacchi, D, Grillo, F, Cazzagon, N, Fabris, L, Cadamuro, M, Cataldo, I, Covelli, C, Mangia, A, Guido, M, Sarcognato S., Sacchi D., Grillo F., Cazzagon N., Fabris L., Cadamuro M., Cataldo I., Covelli C., Mangia A., Guido M., Sarcognato, S, Sacchi, D, Grillo, F, Cazzagon, N, Fabris, L, Cadamuro, M, Cataldo, I, Covelli, C, Mangia, A, Guido, M, Sarcognato S., Sacchi D., Grillo F., Cazzagon N., Fabris L., Cadamuro M., Cataldo I., Covelli C., Mangia A., and Guido M.
- Abstract
Autoimmune cholestatic liver diseases are rare hepato-biliary disorders characterized by a progressive, inflammatory destruction of bile ducts. Primary biliary cholangitis (PBC) and primary sclerosing cholangitis (PSC) are the main autoimmune cholestatic liver diseases. Both may evolve into secondary biliary cirrhosis and its complications.Therapeutic options are limited and liver transplantation remains the only definitive treatment for PBC and PSC. Most PBC and PSC patients have a typical presentation, which does not require liver biopsy. However, in routine clinical practice, important variants or specific subgroups that benefit from liver biopsy for proper management may be observed. Herein, we provide a general overview of clinical and pathological characteristic of PBC and PSC, highlighting the most important features for routine diagnostic practice.
- Published
- 2021
5. Optimal Deployment of Pressure Reduction Valves for Leakage Minimization in Urban Water Distribution Systems
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Covelli, C, Cozzolino, L, Morte, R Della, Pianese, D, and Proceedings of the 34th World Congress of the International Association for Hydro-Environment Research and Engineering: 33rd Hydrology and Water Resources Symposium and 10th Conference on Hydraulics in Water Engineering
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- 2011
6. Benign biliary neoplasms and biliary tumor precursors
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Sarcognato, S, Sacchi, D, Fassan, M, Fabris, L, Cadamuro, M, Zanus, G, Cataldo, I, Covelli, C, Capelli, P, Furlanetto, A, Guido, M, Sarcognato, S, Sacchi, D, Fassan, M, Fabris, L, Cadamuro, M, Zanus, G, Cataldo, I, Covelli, C, Capelli, P, Furlanetto, A, and Guido, M
- Abstract
Benign biliary tumor are common lesions that are often an incidental finding in subjects who undergo medical imaging tests for other conditions. Most are true neoplasms while few result from reactive or malformative proliferation. Benign tumors have no clinical consequences, although the premalignant nature or potential for malignant transformation is of concern in some cases. The main practical problem for pathologists is the need to differentiate them from malignant biliary tumours, which is not always straightforward. Premalignant lesions of the bile duct have been described, although their incidence has been poorly characterized. These lesions include biliary mucinous cystic neoplasms, intraductal papillary neoplasms of the bile duct, and biliary intraepithelial neoplasia. In this article, histopathology of benign biliary tumors and biliary tumor precursors is discussed, with a focus on the main diagnostic criteria.
- Published
- 2021
7. Collagen proportionate area predicts clinical outcomes in patients with alcohol-related liver disease
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Israelsen, M., Guerrero Misas, M., Koutsoumourakis, A., Huang, Y., Thiele, M., Hall, A., Rasmussen, D., Covelli, C., Buzzetti, E., Prat, L. I., Roccarina, D., Detlefsen, S., Luong, T. V., Quaglia, A., Krag, A., Jeffrey, G., Pinzani, M., and Tsochatzis, E. A.
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Adult ,Liver Cirrhosis ,Male ,Biopsy ,Middle Aged ,Aged ,Cohort Studies ,Collagen ,Female ,Humans ,Prognosis ,Prospective Studies ,Retrospective Studies ,Severity of Illness Index - Abstract
BACKGROUND: No prognostic tools are established for alcohol-related liver disease (ALD). Collagen proportionate area (CPA) measurement is a technique that quantifies fibrous tissue in liver biopsies using digital image analysis.AIM: To assess the predictive value of CPA on hepatic decompensation and liver-related mortality in ALD METHODS: In a multicentre cohort study, we included 386 patients with biopsy-verified ALD and with long-term follow-up. In the development cohort of 276 patients, we assessed the predictors of hepatic decompensation and liver-related death in standard and competing risk multivariable Cox regression analyses. The results were validated in an independent prospective cohort of 110 patients, where CPA was also correlated with liver stiffness measurement (LSM).RESULTS: In the development cohort, 231 (84%) patients had early/compensated ALD (non-cirrhotic or compensated cirrhosis) and 45 (16%) had decompensated cirrhosis. In the validation cohort, all patients had early/compensated ALD. Independent predictors of liver-related mortality were higher CPA values (HR = 1.04, 95% CI 1.02-1.04) and advanced fibrosis (HR = 2.80, 95% CI 1.29-6.05) with similar results in standard and competing risk multivariable Cox regression analysis. In early/compensated ALD, CPA was the only independent predictor of hepatic decompensation and liver-related death (HR = 1.08, 95% CI 1.06-1.11). In the prospective cohort, we validated that CPA independently predicts hepatic decompensation in early/compensated ALD. The predictive power of CPA and LSM was equally strong.CONCLUSIONS: CPA predicts liver-related mortality in ALD and hepatic decompensation and/or liver-related death in early/compensated ALD. Traditional histological assessment may benefit from the addition of CPA to the evaluation of ALD.
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- 2020
8. Collagen proportionate area is an independent predictor of short and long-term survival in patients with alcoholic hepatitis
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Misas, M.G., primary, Koutsoumarakis, A., additional, Hall, A., additional, Covelli, C., additional, Buzzetti, E., additional, Prat, L.I., additional, Roccarina, D., additional, Luong, T.V., additional, Pinzani, M., additional, and Tsochatzis, E., additional
- Published
- 2018
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9. Collagen proportionate área is an independent predictor of long term outcome in patients with alcoholic liver disease
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Misas, M.G., primary, Koutsoumarakis, A., additional, Huang, Y., additional, Thiele, M., additional, Hall, A., additional, Covelli, C., additional, Buzzetti, E., additional, Prat, L.I., additional, Roccarina, D., additional, Detlefsen, S., additional, Luong, T.V., additional, Jeffrey, G., additional, Pinzani, M., additional, and Tsochatzis, E., additional
- Published
- 2018
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10. P.10.8 T-HELPER LYMPHOCYTES AND MAST CELLS: AN IMMUNOHISTOCHEMICAL TOOL FOR NON-CELIAC GLUTEN SENSITIVITY?
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Losurdo, G., primary, Piscitelli, D., additional, Pezzuto, F., additional, Fortarezza, F., additional, Covelli, C., additional, Marra, A., additional, Principi, M.B., additional, Amoruso, A., additional, Iannone, A., additional, Ierardi, E., additional, and Di Leo, A., additional
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- 2018
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11. Innesco del piping nei rilevati arginali
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Covelli, C., Cozzolino, L., Cimorelli, L., Della Morte, R., Molino, B., and Pianese, D.
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RISCHIO IDRAULICO ,PIPING ,RISCHIO IDRAULICO,RILEVATI ARGINALI,MOTI DI FILTRAZIONE,PIPING,ALLUVIONI ,ALLUVIONI ,RILEVATI ARGINALI ,MOTI DI FILTRAZIONE - Published
- 2016
12. Collagen proportion area is an independent predictor of longterm outcome in patients with non-alcoholic fatty liver disease
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Buzzetti, E., primary, Hall, A., additional, Ekstedt, M., additional, Manuguerra, R., additional, Covelli, C., additional, Misas, M.G., additional, Luong, T.V., additional, Kechagias, S., additional, Manesis, E.K., additional, Pinzani, M., additional, Dhillon, A.P., additional, and Tsochatzis, E., additional
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- 2017
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13. Optimal Positioning and Sizing of Detention Tanks within Urban Drainage Networks
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Cimorelli, L., primary, Morlando, F., additional, Cozzolino, L., additional, Covelli, C., additional, Della Morte, R., additional, and Pianese, D., additional
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- 2016
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14. SAT-456 - Collagen proportionate área is an independent predictor of long term outcome in patients with alcoholic liver disease
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Misas, M.G., Koutsoumarakis, A., Huang, Y., Thiele, M., Hall, A., Covelli, C., Buzzetti, E., Prat, L.I., Roccarina, D., Detlefsen, S., Luong, T.V., Jeffrey, G., Pinzani, M., and Tsochatzis, E.
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- 2018
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15. PS-070 - Collagen proportionate area is an independent predictor of short and long-term survival in patients with alcoholic hepatitis
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Misas, M.G., Koutsoumarakis, A., Hall, A., Covelli, C., Buzzetti, E., Prat, L.I., Roccarina, D., Luong, T.V., Pinzani, M., and Tsochatzis, E.
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- 2018
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16. EVALUATION OF DRY STEAM PRECONDITIONING ON SWITCHGRASS PELLET QUALITY METRICS.
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Covelli, C. R., Yi, H., Karamchandani, A., Ciolkosz, D., and Puri, V. M.
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- 2018
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17. Tramadol detection in sports
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Covelli, C., De La Torre, X., and Botre', Francesco
- Published
- 2012
18. Macrophages and mast cells are involved in carotid plaque instability
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Marzullo, A., Marco Ciccone, Covelli, C., Serio, G., and Ribatti, D.
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Male ,Carotid Arteries ,Biopsy ,Macrophages ,Microvessels ,Humans ,Female ,Mast Cells ,Plaque, Atherosclerotic - Abstract
The aim of this study was to evaluate the role of macrophages and mast cells and of microvascular density in atherosclerotic plaques collected from 63 consecutive symptomatic and asymptomatic patients undergoing carotid endarterectomy for carotid disease. Results have shown no statistically significant differences between the two groups as concerns: (i) the degree of stenosis; (ii) the extention of the lipidic core; (iii) the thickness of the fibrous cup; (iv) the inflammatory infiltrate; (v) the degree of calcification; (vi) the intraplaque hemorrhage. Otherwise, statistically significant difference was found in microvascular density, in the number of CD68-positive macrophages and tryptase-positive mast cells in plaques from symptomatic patients, as compared to asymptomatic patients. Overall, this study indicate that although advanced symptomatic and asymptomatic carotid plaques present similar histomorphological characteristics, the degree of macrophage and mast cell infiltration and differences in microvascular density could help to discriminate between symptomatic and asymptomatic patients.
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- 2011
19. P.1.112 Double-blind, randomized study of venlafaxine, clomipramine, and trazodone in geriatric patients with major depression
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Smeraldi, E., primary, Aguglia, A., additional, Cattaneo, M., additional, Cerati, C., additional, Covelli, C., additional, Del Zompo, M., additional, Faravelli, C., additional, Gerrari, G., additional, Ghirlanda, A., additional, Guazzelli, G., additional, Mansi, M., additional, and Ravizza, L., additional
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- 1997
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20. Molecular changes of malignant mesothelioma in the testis and their impact on prognosis: Analyses of two cases
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Serio, G., Pagliarulo, V., Marzullo, A., Punzi, A., Pezzuto, F., MATTIA GENTILE, Pennella, A., Nazzaro, P., Buonadonna, A. L., Covelli, C., Lettini, T., and Scattone, A.
21. Impact of Pre-Analytical Factors on MSI Test Accuracy in Mucinous Colorectal Adenocarcinoma: A Multi-Assay Concordance Study
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Caterina De Luca, Maria D'Armiento, Dario Bruzzese, Gianluca Russo, Mariangela Balestrieri, Antonio Bonfitto, Giancarlo Troncone, Pellegrino Cerino, Matteo Fassan, Maria Triassi, Umberto Malapelle, Filippo Pietrantonio, Claudia Covelli, Pasquale Pisapia, Fabiola Fiordelisi, Francesco Pepe, Paolo Graziano, Fotios Loupakis, Paola Parente, Malapelle, U., Parente, P., Pepe, F., De Luca, C., Cerino, P., Covelli, C., Balestrieri, M., Russo, G., Bonfitto, A., Pisapia, P., Fiordelisi, F., D'Armiento, M., Bruzzese, D., Loupakis, F., Pietrantonio, F., Triassi, M., Fassan, M., Troncone, G., and Graziano, P.
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Male ,0301 basic medicine ,Oncology ,Tissue Fixation ,Colorectal cancer ,microfluidic ,Polymerase Chain Reaction ,law.invention ,0302 clinical medicine ,law ,fully automated RT—PCR ,Medicine ,lcsh:QH301-705.5 ,Polymerase chain reaction ,Pre analytical ,DNA, Neoplasm ,General Medicine ,Middle Aged ,Prognosis ,Adenocarcinoma, Mucinous ,Immunohistochemistry ,Lynch syndrome ,mCRC ,030220 oncology & carcinogenesis ,IHC ,predictive molecular pathology ,Female ,Microsatellite Instability ,Colorectal Neoplasms ,medicine.medical_specialty ,Concordance ,Article ,Diagnosis, Differential ,03 medical and health sciences ,Internal medicine ,Humans ,Aged ,Retrospective Studies ,Tissue Embedding ,business.industry ,Electrophoresis, Capillary ,Microsatellite instability ,medicine.disease ,Colorectal Neoplasms, Hereditary Nonpolyposis ,digestive system diseases ,030104 developmental biology ,lcsh:Biology (General) ,Case-Control Studies ,Neoplastic cell ,business - Abstract
Immunohistochemistry (IHC) and polymerase chain reaction (PCR) and fragment separation by capillary electrophoresis represent the current clinical laboratory standard for the evaluation of microsatellite instability (MSI) status. The importance of reporting MSI status in colorectal cancer is based on its potential for guiding treatment and as a prognostic indicator. It is also used to identify patients for Lynch syndrome testing. Our aim was to evaluate pre-analytical factors, such as age of formalin-fixed and paraffin-embedded (FFPE) block, neoplastic cell percentage, mucinous component, and DNA integrity, that may influence the accuracy of MSI testing and assess the concordance between three different MSI evaluation approaches. We selected the mucinous colorectal cancer (CRC) histotype for this study as it may possibly represent an intrinsic diagnostic issue due to its low tumor cellularity. Seventy-five cases of mucinous CRC and corresponding normal colon tissue samples were retrospectively selected. MMR proteins were evaluated by IHC. After DNA quality and quantity evaluation, the Idylla&trade, and TapeStation 4200 platforms were adopted for the evaluation of MSI status. Seventy-three (97.3%) cases were successfully analyzed by the three methodologies. Overall, the Idylla&trade, platform showed a concordance rate with IHC of 98.0% for microsatellite stable (MSS)/proficient MMR (pMMR) cases and 81.8% for MSI/deficient MMR (dMMR) cases. The TapeStation 4200 system showed a concordance rate with IHC of 96.0% for MSS/pMMR cases and 45.4% for MSI/dMMR cases. The concordance rates of the TapeStation 4200 system with respect to the Idylla&trade, platform were 98.1% for MSS profile and 57.8% for MSI profile. Discordant cases were analyzed using the Titano MSI kit. Considering pre-analytical factors, no significant variation in concordance rate among IHC analyses and molecular systems was observed by considering the presence of an acellular mucus cut-off >, 50% of the tumor area, FFPE year preparation, and DNA concentration. Conversely, the Idylla&trade, platform showed a significant variation in concordance rate with the IHC approach by considering a neoplastic cell percentage >, 50% (p-value = 0.002), and the TapeStation 4200 system showed a significant variation in concordance rate with the IHC approach by considering a DNA integrity number (DIN) &ge, 4 as cut-off (p-value = 0.009). Our data pinpoint a central role of the pre-analytical phase in the diagnostic outcome of MSI testing in CRC.
- Published
- 2020
22. Optimal Regulation of Pumping Station in Water Distribution Networks Using Constant and Variable Speed Pumps: A Technical and Economical Comparison
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Domenico Pianese, Carmine Covelli, Luigi Cimorelli, B. Molino, Cimorelli, L., Covelli, C., Molino, B., and Pianese, D.
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Water pumping ,variable speed pump ,Control and Optimization ,Computer science ,020209 energy ,0208 environmental biotechnology ,Scheduling (production processes) ,Energy Engineering and Power Technology ,02 engineering and technology ,lcsh:Technology ,Automotive engineering ,Order (exchange) ,energy saving ,0202 electrical engineering, electronic engineering, information engineering ,Electrical and Electronic Engineering ,Engineering (miscellaneous) ,pumping station ,Atmosphere (unit) ,lcsh:T ,Renewable Energy, Sustainability and the Environment ,Purchasing ,020801 environmental engineering ,Variable (computer science) ,Greenhouse gas ,optimal scheduling ,water distribution network ,Constant (mathematics) ,Energy (miscellaneous) - Abstract
Greenhouse gas emission is one of the main environmental issues of today, and energy savings in all industries contribute to reducing energy demand, implying, in turn, less carbon emissions into the atmosphere. In this framework, water pumping systems are one of the most energy-consuming activities. The optimal regulation of pumping systems with the use of variable speed drives is gaining the attention of designers and managing authorities. However, optimal management and operation of pumping systems is often performed, employing variable speed drives without considering if the energy savings are enough to justify their purchasing and installation costs. In this paper, the authors compare two optimal pump scheduling techniques, optimal regulation of constant speed pumps by an optimal ON/OFF sequence and optimal regulation with a variable speed pump. Much of the attention is devoted to the analysis of the costs involved in a hypothetical managing authority for the water distribution system in order to determine whether the savings in operating costs is enough to justify the employment of variable speed drives.
- Published
- 2020
23. Mismatch repair proteins and microsatellite instability in solid pseudopapillary neoplasm of the pancreas
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Francesco Pepe, Paola Parente, Umberto Malapelle, Claudia Covelli, Pasquale Pisapia, Fabiola Fiordelisi, Covelli, C., Parente, P., Pepe, F., Pisapia, P., Fiordelisi, F., and Malapelle, U.
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Adult ,Male ,DNA Mismatch Repair ,Polymerase Chain Reaction ,Young Adult ,Biomarkers, Tumor ,medicine ,Humans ,Neoplasm ,Mismatch Repair Endonuclease PMS2 ,Hepatology ,business.industry ,Gastroenterology ,Microsatellite instability ,Middle Aged ,medicine.disease ,Immunohistochemistry ,DNA-Binding Proteins ,Pancreatic Neoplasms ,MutS Homolog 2 Protein ,medicine.anatomical_structure ,Cancer research ,Female ,Microsatellite Instability ,DNA mismatch repair ,Neoplasm Grading ,MutL Protein Homolog 1 ,Pancreas ,business - Abstract
Solid pseudopapillary neoplasms (SPNs) are rare solid pancreatic tumors mainly affecting young women. Despite the high percentage of favorable prognosis, they are considered as low grade malignant neoplasms, and metastases occur in 5%−15% of patients. Almost all SPNs (95%) have somatic activating mutations in the β-catenin gene [1]. β-catenin plays a crucial role in cell proliferation and differentiation via Wnt signaling pathway and interacts with E-cadherin and α-catenin for the regulation of cell adhesion and growth [2]. Another relevant genetic alteration in pancreatic neoplasms is represented by the DNA mismatch repair deficiency (dMMR). dMMR is strongly associated with a microsatellite instability (MSI) status as a result of an alteration in the lengths of microsatellites due to deletion/insertion of repeating units in tumor DNA [3]. dMMR was observed in approximately 1%−2% of patients with pancreatic adenocarcinoma [4]. To date, the “gold standard” for the evaluation of protein integrity of MMR is immunohistochemistry (IHC), showing an analytical sensitivity >90%, specificity of 100% and predictive value of 97% for microsatellite stability (MSS) and 100% for MSI [5]. The study aimed to investigate the expression of MMR proteins and the MSI status in pancreatic SPNs.
- Published
- 2019
24. Tramadol and Cycling: Is It the End of a "Painful" Relationship? An Insight From 60,802 Doping-Control Samples From 2012 to 2020.
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Zandonai T, Peiró AM, Covelli C, de la Torre X, and Botré F
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- Humans, Bicycling, Athletes, Doping in Sports, Tramadol, Sports
- Abstract
Purpose: To assess the prevalence of tramadol use among athletes from 2012 to 2020., Methods: All urine samples were collected from national and international in-competition doping-control tests that took place in Italy between 2012 and 2020. The analysis of the samples was performed by gas chromatography coupled with mass spectrometry with electronic ionization and acquisition in selected ion monitoring. The cutoff tramadol concentration was >50 ng/mL., Results: Of the 60,802 in-competition urine samples we analyzed, 1.2% (n = 759) showed tramadol intake, with 84.2% (n = 637) of these coming from cyclists and 15.8% (n = 122) from other sports. In cycling, a strong and significant negative correlation was found (r = -.738; P = .003), showing a decrease of tramadol use compared with the other sports., Conclusions: The decrease in tramadol prevalence in cycling in the last years may be due to (1) the deterrent action of antidoping regulations and (2) the fact that tramadol may not have any actual ergogenic effect on performance.
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- 2022
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25. Critical aspects of microsatellite instability testing in endometrial cancer: a comparison study.
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Libera L, Sahnane N, Pepe F, Pisapia P, De Luca C, Russo G, Parente P, Covelli C, Chiaravalli AM, Sessa F, Malapelle U, and Furlan D
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- DNA Mismatch Repair, Female, Humans, Immunohistochemistry, Microsatellite Instability, Microsatellite Repeats, Colorectal Neoplasms genetics, Endometrial Neoplasms diagnosis, Endometrial Neoplasms genetics
- Abstract
The identification of mismatch repair deficient (dMMR) and microsatellite unstable (MSI) endometrial cancers (ECs) is important in screening, diagnosis, and therapeutic stratification of patients. We compared the diagnostic performance of 4 MSI molecular tests based on fragment length assay in capillary electrophoresis (OncoMate™ MSI assay, Promega) and in microcapillary electrophoresis (TapeStation 4200, Agilent); with high-resolution melting (HRM) analysis approaches (Idylla™ MSI Test, Biocartis; EasyPGX® ready MSI, Diatech Pharmacogenetics) on a series of 56 ECs, which was well characterized for MMR status with immunohistochemical approach (IHC, nonmolecular reference test). The concordance of fluorescence capillary electrophoresis with IHC (AUC 0.98) was higher respect to the other molecular methodologies. Otherwise, HRM approaches and microcapillary electrophoresis platform failed to detect MSI-ECs showing minimal microsatellite shifts. In conclusion, in colorectal site, several technologies are eligible for MSI test, whereas in ECs, MSI test should be based on fluorescent capillary electrophoresis as it identifies a higher proportion of cases that could be misdiagnosed with other strategies., (Copyright © 2022 Elsevier Inc. All rights reserved.)
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- 2022
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26. Collagen proportionate area predicts long-term mortality in patients with alcoholic hepatitis.
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Israelsen M, Misas MG, Koutsoumourakis A, Hall A, Covelli C, Buzzetti E, Prat LI, Roccarina D, Luong TV, Quaglia A, Pinzani M, and Tsochatzis EA
- Subjects
- Collagen, Humans, Liver Cirrhosis, Prognosis, Retrospective Studies, Severity of Illness Index, Hepatitis, Alcoholic
- Abstract
Background and Aims: There are several short-term prognostic scores for alcoholic hepatitis (AH) that combine demographical and biochemical parameters. The extent of liver fibrosis may also be relevant to the prognosis of AH with potential added value. We evaluated collagen proportionate area (CPA) as a predictor of short and long-term mortality in AH., Methods: We retrospectively included patients with biopsy-verified AH. Clinical, laboratory and outcome data were collected. CPA and five AH scores were calculated: Maddrey's DF, MELD, GAHS, ABIC, and the Lille Model. Predictors of short and long-term all-cause mortality were assessed using Cox regression analysis., Results: We included 140 patients with AH. In total, 67 (48%) patients died after a median follow-up of 66 (IQR 102) months, with 17 (12%) dying within the first 90-days. CPA was not a predictor of 90-days mortality and had no additional value to the prognostic AH scores on short-term mortality. However, CPA predicted long-term mortality independently of prognostic AH scores. Importantly, CPA and abstinence from alcohol were independent predictors of long-term mortality in patients alive 90 days after the biopsy., Conclusion: CPA predicts long-term mortality in patients with AH independently of abstinence from alcohol but has no prognostic value on short-term mortality., Competing Interests: Declaration of Competing Interest None declared., (Copyright © 2021. Published by Elsevier Ltd.)
- Published
- 2022
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27. When idiopathic retroperitoneal fibrosis mimics Castleman disease: a challenging differential diagnosis.
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Covelli C, Carosi I, Graziano P, and Ascani S
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- Diagnosis, Differential, Humans, Castleman Disease diagnosis, Retroperitoneal Fibrosis diagnostic imaging
- Abstract
Competing Interests: Competing interests: None declared.
- Published
- 2022
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28. Lump on the scalp of a child arising over a previous parietal fracture: growing skull fracture or post-traumatic lipoma?
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Tewfik K, Covelli C, Rossini M, and Burlini D
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- Child, Female, Humans, Magnetic Resonance Imaging, Scalp pathology, Tomography, X-Ray Computed, Lipoma complications, Lipoma diagnostic imaging, Lipoma surgery, Skull Fractures complications, Skull Fractures diagnostic imaging
- Abstract
A young girl was referred to our Institution for the appearance of a painless soft swelling in the right parietal region of the scalp. In the same site, the patient had a parietal bone fracture 1 year ago. In the suspicion of a growing skull fracture, the patient underwent radiological investigations. Ultrasound showed a soft, poorly vascularised swelling with parenchymatous content. The skull X-ray showed an apparent healing of the previous fracture. CT scan and MRI confirmed the correct healing of the fracture and described the presence of a lipomatous mass. The mass was surgically removed and histology confirmed the diagnosis of encapsulated lipoma. The postoperative period was uneventful, with no evidence of infections or recurrence in the 6-month follow-up., Competing Interests: Competing interests: None declared., (© BMJ Publishing Group Limited 2022. No commercial re-use. See rights and permissions. Published by BMJ.)
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- 2022
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29. Multidisciplinary Management of an Orbitocranial Penetrating Injury by a Pencil in a Paediatric Patient - A Case Report.
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Tewfik K, Covelli C, Rossini M, Peta C, and Burlini D
- Abstract
Rationale: Orbitocranial penetrating injuries can accidentally occur in children while handling pencils and can cause severe sequelae such as ocular damage, brain lesion, intracranial haemorrhage, and infections., Patient Concerns: We report the case of a 7-year-old child with an orbitocranial penetrating injury by a pencil, initially gone undetected, that caused a direct damage to the optic nerve., Diagnosis: Computed tomography scan with contrast detected the foreign body and the presence of a lesion of the left internal carotid artery., Treatment: Angiography was performed to treat the vascular lesion and to prevent haemorrhage. Subsequently, a craniotomy was performed to assist the extraction of the pencil from the entry wound and to remove residual fragments., Outcomes: Left eye vision was lost. The 1-year follow-up was uneventful., Take-Away Lessons: Operative angiography is mandatory before the surgical extraction of the orbitocranial penetrating injury in case of documented intracranial vessel damage., Competing Interests: There are no conflicts of interest., (Copyright: © 2022 Annals of Maxillofacial Surgery.)
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- 2022
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30. Correction to: A New Intraepithelial γδ T-Lymphocyte Marker for Celiac Disease Classification in Formalin-Fixed Paraffin-Embedded (FFPE) Duodenal Biopsies.
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Popp A, Taavela J, Graziano P, Parente P, Covelli C, Lamacchia C, Andriulli A, Mäki M, and Isola J
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- 2021
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31. A New Intraepithelial γδ T-Lymphocyte Marker for Celiac Disease Classification in Formalin-Fixed Paraffin-Embedded (FFPE) Duodenal Biopsies.
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Popp A, Taavela J, Graziano P, Parente P, Covelli C, Lamacchia C, Andriulli A, Mäki M, and Isola J
- Subjects
- Antibodies, Monoclonal, Biomarkers chemistry, Duodenum metabolism, Duodenum pathology, Immunohistochemistry, Receptors, Antigen, T-Cell, gamma-delta genetics, Celiac Disease diagnosis, Formaldehyde, Paraffin Embedding, Receptors, Antigen, T-Cell, gamma-delta metabolism, T-Lymphocytes physiology, Tissue Fixation
- Abstract
Background: The histopathologic diagnosis of celiac disease (CD) may be challenging when the duodenal biopsies mucosal injury is limited. Intraepithelial T-lymphocytes (IELs) can be useful to characterize the degree of mucosal inflammation. A small fraction of IELs expresses the γδ T-cell receptor (named γδ-IELs), whose density, determined by flow cytometry or frozen section immunohistochemistry (IHC), is a specific marker for CD., Aim: To establish a new IHC assay for γδ-IELs applicable to formalin-fixed paraffin-embedded (FFPE) duodenal biopsies., Methods: We analyzed γδ-IELs using IHC in 138 duodenal biopsies using a standard IHC staining protocol with a new monoclonal antibody H-41. IELs were quantitated with digital image analysis., Results: Compared to those in non-celiac controls (n = 51), γδ-IEL density was significantly increased in newly diagnosed celiac disease patients (n = 22, p < 0.0001). In ROC-curve analysis, the cutoff of 6.5 γδ-IELs/100 enterocytes distinguished optimally active CD patients from non-celiac controls (sensitivity 96%, specificity 95%). γδ-IEL density in CD patients on a gluten-free diet (n = 53) were also higher than in controls (p < 0.0001), but lower than those in newly diagnosed CD (p < 0.0001). The diagnostic value of γδ-IELs outperformed that of CD3 + IELs in both patient groups. γδ-IELs were better than CD3 + IELs distinguishing between celiac disease and conditions histologically mimicking celiac disease (n = 12)., Conclusions: Intraepithelial γδ T-lymphocytes can be stained and quantitated reliably in FFPE duodenal biopsies. The results showed excellent specificity and sensitivity for celiac disease. The new IHC method of detection of γδ-IELs is a promising addition to the routine histopathologic assessment methodology of celiac disease., (© 2020. The Author(s).)
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- 2021
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32. Primary Epithelioid Hemangioma of the Central Nervous System: A Case Report and Review of the Literature.
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Covelli C, Parente P, Icolaro N, Dimitri LMC, Vigna B, Popolizio T, and Graziano P
- Subjects
- Aged, Brain Neoplasms diagnostic imaging, Female, Hemangioendothelioma, Epithelioid diagnostic imaging, Humans, Magnetic Resonance Imaging, Brain Neoplasms pathology, Hemangioendothelioma, Epithelioid pathology
- Published
- 2021
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33. Evaluation of Micro Satellite Instability and Mismatch Repair Status in Different Solid Tumors: A Multicenter Analysis in a Real World Setting.
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Malapelle U, Parente P, Pepe F, De Luca C, Pisapia P, Sgariglia R, Nacchio M, Gragnano G, Russo G, Conticelli F, Bellevicine C, Vigliar E, Iaccarino A, Covelli C, Balistreri M, Clemente C, Perrone G, Danza A, Scaramuzzi F, Fassan M, Troncone G, and Graziano P
- Subjects
- Biomarkers, Tumor analysis, DNA Repair Enzymes analysis, Digestive System Neoplasms enzymology, Digestive System Neoplasms pathology, Endometrial Neoplasms enzymology, Endometrial Neoplasms genetics, Endometrial Neoplasms pathology, Female, Genital Neoplasms, Female enzymology, Genital Neoplasms, Female pathology, Humans, Immunohistochemistry, Italy, Male, Microfluidic Analytical Techniques, Molecular Diagnostic Techniques, Ovarian Neoplasms enzymology, Ovarian Neoplasms genetics, Ovarian Neoplasms pathology, Pancreatic Neoplasms enzymology, Pancreatic Neoplasms genetics, Pancreatic Neoplasms pathology, Polymerase Chain Reaction, Predictive Value of Tests, Prostatic Neoplasms enzymology, Prostatic Neoplasms pathology, Reproducibility of Results, Retrospective Studies, Stomach Neoplasms enzymology, Stomach Neoplasms genetics, Stomach Neoplasms pathology, DNA Mismatch Repair, Digestive System Neoplasms genetics, Genital Neoplasms, Female genetics, Microsatellite Instability, Prostatic Neoplasms genetics
- Abstract
Immune-checkpoint inhibitors (ICIs) play a key role in the treatment of advanced stage colorectal cancer (CRC) patients featuring a deficient DNA mismatch repair (dMMR) system or a high microsatellite instability (MSI-H) profile. However, beyond the established role in CRC patients, ICIs have highly proven efficacy in other solid tumors featuring MSI-H/dMMR status represented by endometrial, gastric, ovarian, prostatic, and pancreatic carcinomas (EC, GC, OC, PrC, and PaC). Our aim was to compare the concordance rates among the Idylla™ MSI test, TapeStation 4200, and immunohistochemical (IHC) analysis in assessing MSI-H/dMMR status in EC, GC, OC, PrC, and PaC patients. The Sanger sequencing-based Titano MSI test was used in discordant cases. One hundred and eighty-five cases ( n = 40 PrC, n = 39 GC, n = 38 OC, n = 35 PaC, and n = 33 EC) were retrospectively selected. MMR protein expression was evaluated by IHC. After DNA quality and quantity evaluations, the Idylla
TM and TapeStation 4200 platforms were adopted for the evaluation of MSI status. Remarkably, compared to IHC, the Idylla™ platform achieved a global concordance rate of 94.5% (154/163) for the microsatellite stable (MSS)/proficient MMR (pMMR) cases and 77.3% (17/22) for the MSI-H/dMMR cases. Similarly, a global concordance rate of 91.4% (149/163) and 68.2% (15/22) for MSS/pMMR and MSI-H/dMMR cases was also identified between IHC and the TapeStation 4200 microfluidic system. In addition, a global concordance of 93.1% (148/159) and 69.2% (18/26) for MSS/pMMR and MSI-H/dMMR cases was observed between the Idylla™ and TapeStation 4200 platforms. Discordant cases were analyzed using the Titano MSI kit. Overall, our data pinpointed a central role for molecular techniques in the diagnostic evaluation of dMMR/MSI-H status not only in CRC patients but also in other types of solid tumors.- Published
- 2021
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34. Synchronous primary gastric triple-hit high-grade B-cell lymphoma and gastric adenocarcinoma: endoscopic and pathological findings.
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Covelli C, Parente P, Trombetta D, and Graziano P
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- Humans, Adenocarcinoma diagnostic imaging, Adenocarcinoma surgery, Lymphoma, B-Cell, Neoplasms, Multiple Primary diagnostic imaging, Neoplasms, Multiple Primary surgery, Stomach Neoplasms diagnostic imaging, Stomach Neoplasms surgery
- Abstract
Competing Interests: Competing interests: None declared.
- Published
- 2021
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35. Pathology of autoimmune hepatitis.
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Covelli C, Sacchi D, Sarcognato S, Cazzagon N, Grillo F, Baciorri F, Fanni D, Cacciatore M, Maffeis V, and Guido M
- Subjects
- Autoantibodies, Female, Humans, Immunosuppression Therapy, Hepatitis, Autoimmune diagnosis
- Abstract
Autoimmune hepatitis (AIH) is a relatively rare non-resolving chronic liver disease, which mainly affects women. It is characterized by hypergammaglobulinemia, circulating autoantibodies, interface hepatitis on liver histology and a favourable response to immunosuppression. The putative mechanism for the development of autoimmune hepatitis is thought to be the interaction between genetic predisposition, environmental triggers and failure of the native immune system., AIH still remains a major diagnostic and therapeutic challenge, mainly because it is a very heterogeneous disease. Prompt and timely diagnosis is crucial since, if left untreated, AIH has a high mortality rate. Histological demonstration of hepatitis is required for the diagnosis of AIH and, therefore, liver biopsy is mandatory in the initial diagnostic work-up, before treatment. In this review, we summarize the histological features of AIH with the main aim of highlighting the most important clinical-pathological hallmarks useful in the routine diagnostic practice., (Copyright © 2021 Società Italiana di Anatomia Patologica e Citopatologia Diagnostica, Divisione Italiana della International Academy of Pathology.)
- Published
- 2021
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36. Autoimmune biliary diseases: primary biliary cholangitis and primary sclerosing cholangitis.
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Sarcognato S, Sacchi D, Grillo F, Cazzagon N, Fabris L, Cadamuro M, Cataldo I, Covelli C, Mangia A, and Guido M
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- Humans, Autoimmune Diseases complications, Cholangitis, Sclerosing complications, Cholangitis, Sclerosing diagnosis, Cholangitis, Sclerosing epidemiology, Liver Cirrhosis, Biliary complications, Liver Cirrhosis, Biliary diagnosis
- Abstract
Autoimmune cholestatic liver diseases are rare hepato-biliary disorders characterized by a progressive, inflammatory destruction of bile ducts. Primary biliary cholangitis (PBC) and primary sclerosing cholangitis (PSC) are the main autoimmune cholestatic liver diseases. Both may evolve into secondary biliary cirrhosis and its complications. Therapeutic options are limited and liver transplantation remains the only definitive treatment for PBC and PSC., Most PBC and PSC patients have a typical presentation, which does not require liver biopsy. However, in routine clinical practice, important variants or specific subgroups that benefit from liver biopsy for proper management may be observed. Herein, we provide a general overview of clinical and pathological characteristic of PBC and PSC, highlighting the most important features for routine diagnostic practice., (Copyright © 2021 Società Italiana di Anatomia Patologica e Citopatologia Diagnostica, Divisione Italiana della International Academy of Pathology.)
- Published
- 2021
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37. Italian cost analysis of free flap surgery in head and neck reconstruction using the activity-based costing (ABC).
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Tewfik K, Chiarelli P, Copelli C, Pederneschi N, Cassano L, Manfuso A, Covelli C, and Longo F
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- Delivery of Health Care economics, Female, Humans, Italy epidemiology, Male, Middle Aged, Retrospective Studies, Costs and Cost Analysis methods, Costs and Cost Analysis statistics & numerical data, Free Tissue Flaps classification, Free Tissue Flaps economics, Head and Neck Neoplasms economics, Head and Neck Neoplasms surgery, Plastic Surgery Procedures economics, Plastic Surgery Procedures methods
- Abstract
The aim of the study is to evaluate costs of free flap surgery for head and neck (H & N) reconstructions using the time-driven activity-based costing (ABC) method and to compare them with the refund provided by the Italian National Health System (NHS) amounting to 11,891€. We retrospectively selected 29 consecutive patients underwent free flap reconstruction in 2013 at IRCCS Casa Sollievo della Sofferenza. Patients were divided into three groups: Group 1 (n = 10) included patients receiving radial forearm free flap (RFFF), Group 2 (n = 10) receiving anterolateral thigh (ALT) free flap, and Group 3 (n = 9) composed of patients having fibular free flap. For each patient, costs were calculated using the ABC and divided into instay, surgical, and services costs. We observed an overall mean total cost of 27,802.40€. The mean costs related to hospital stay were 9,800.70€. The mean costs for surgery were 13,097.60€ and amounted to 4,904.10€ for services. RFFF appears to be less costing (25,175.40€) compared with ALT (29,191.60€) and fibula free flap (29,040.20€). ABC is an appropriate method to determine actual costs of free flap surgery by correctly allocating the resources used. The Italian NHS tariff seems to be inadequate to cover the real cost of this type of surgery., Competing Interests: Declaration of Competing Interest None declared., (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Published
- 2021
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38. Benign biliary neoplasms and biliary tumor precursors.
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Sarcognato S, Sacchi D, Fassan M, Fabris L, Cadamuro M, Zanus G, Cataldo I, Covelli C, Capelli P, Furlanetto A, and Guido M
- Subjects
- Diagnostic Imaging, Humans, Pathologists, Bile Duct Neoplasms diagnosis, Bile Duct Neoplasms epidemiology, Carcinoma in Situ, Precancerous Conditions epidemiology
- Abstract
Benign biliary tumor are common lesions that are often an incidental finding in subjects who undergo medical imaging tests for other conditions. Most are true neoplasms while few result from reactive or malformative proliferation. Benign tumors have no clinical consequences, although the premalignant nature or potential for malignant transformation is of concern in some cases. The main practical problem for pathologists is the need to differentiate them from malignant biliary tumours, which is not always straightforward., Premalignant lesions of the bile duct have been described, although their incidence has been poorly characterized. These lesions include biliary mucinous cystic neoplasms, intraductal papillary neoplasms of the bile duct, and biliary intraepithelial neoplasia. In this article, histopathology of benign biliary tumors and biliary tumor precursors is discussed, with a focus on the main diagnostic criteria., (Copyright © 2021 Società Italiana di Anatomia Patologica e Citopatologia Diagnostica, Divisione Italiana della International Academy of Pathology.)
- Published
- 2021
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39. Collagen proportionate area predicts clinical outcomes in patients with alcohol-related liver disease.
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Israelsen M, Guerrero Misas M, Koutsoumourakis A, Huang Y, Thiele M, Hall A, Rasmussen D, Covelli C, Buzzetti E, Prat LI, Roccarina D, Detlefsen S, Luong TV, Quaglia A, Krag A, Jeffrey G, Pinzani M, and Tsochatzis EA
- Subjects
- Adult, Aged, Biopsy, Cohort Studies, Female, Humans, Male, Middle Aged, Prognosis, Prospective Studies, Retrospective Studies, Severity of Illness Index, Collagen analysis, Liver Cirrhosis pathology
- Abstract
Background: No prognostic tools are established for alcohol-related liver disease (ALD). Collagen proportionate area (CPA) measurement is a technique that quantifies fibrous tissue in liver biopsies using digital image analysis., Aim: To assess the predictive value of CPA on hepatic decompensation and liver-related mortality in ALD METHODS: In a multicentre cohort study, we included 386 patients with biopsy-verified ALD and with long-term follow-up. In the development cohort of 276 patients, we assessed the predictors of hepatic decompensation and liver-related death in standard and competing risk multivariable Cox regression analyses. The results were validated in an independent prospective cohort of 110 patients, where CPA was also correlated with liver stiffness measurement (LSM)., Results: In the development cohort, 231 (84%) patients had early/compensated ALD (non-cirrhotic or compensated cirrhosis) and 45 (16%) had decompensated cirrhosis. In the validation cohort, all patients had early/compensated ALD. Independent predictors of liver-related mortality were higher CPA values (HR = 1.04, 95% CI 1.02-1.04) and advanced fibrosis (HR = 2.80, 95% CI 1.29-6.05) with similar results in standard and competing risk multivariable Cox regression analysis. In early/compensated ALD, CPA was the only independent predictor of hepatic decompensation and liver-related death (HR = 1.08, 95% CI 1.06-1.11). In the prospective cohort, we validated that CPA independently predicts hepatic decompensation in early/compensated ALD. The predictive power of CPA and LSM was equally strong., Conclusions: CPA predicts liver-related mortality in ALD and hepatic decompensation and/or liver-related death in early/compensated ALD. Traditional histological assessment may benefit from the addition of CPA to the evaluation of ALD., (© 2020 The Authors. Alimentary Pharmacology & Therapeutics published by John Wiley & Sons Ltd.)
- Published
- 2020
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40. Diagnosis of Hodgkin Lymphoma from Cell Block: A Reliable and Helpful Tool in "Selected" Diagnostic Practice.
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Parente P, Covelli C, Zanelli M, Trombetta D, Carosi I, Carbonelli C, Sperandeo M, Mastracci L, Biancofiore G, Zizzo M, Taurchini M, Ascani S, and Graziano P
- Abstract
Background: The diagnosis of lymphoma requires surgical specimens to perform morphological evaluation, immunohistochemical and molecular analyses. Ultrasound-guided fine needle aspiration may represent an appropriate first approach to obtain cytological samples in impalpable lesions and/or in patients unsuitable for surgical procedures. Although cytology has intrinsic limitations, the cell block method may increase the possibility of achieving an accurate diagnosis., Methods: We retrospectively selected a total of 47 ultrasound-guided fine needle aspiration and drainage samples taken from patients with effusion and deep-seated lesions which are clinically suspicious in terms of malignancy., Results: In 27 cases, both cell block and conventional cytology were performed: 21/27 cell blocks were adequate for the diagnosis of lymphoma and suitable for immunocytochemistry and molecular analyses vs. 12/20 samples to which only conventional cytology was applied. Moreover, in five patients we were able to make a diagnosis of Hodgkin lymphoma with the cell block (CB) technique., Conclusions: Contrary to conventional cytology, the cell block method may allow immunocytochemistry and molecular studies providing useful information for the diagnosis and subtypization of lymphoma in patients unsuitable for surgical procedure or with deep-seated lesions or extra-nodal diseases; additionally, it is a daily, simple and helpful approach. Moreover, we describe the usefulness of cell blocks in the diagnosis of Hodgkin lymphoma.
- Published
- 2020
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41. Impact of Pre-Analytical Factors on MSI Test Accuracy in Mucinous Colorectal Adenocarcinoma: A Multi-Assay Concordance Study.
- Author
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Malapelle U, Parente P, Pepe F, De Luca C, Cerino P, Covelli C, Balestrieri M, Russo G, Bonfitto A, Pisapia P, Fiordelisi F, D'Armiento M, Bruzzese D, Loupakis F, Pietrantonio F, Triassi M, Fassan M, Troncone G, and Graziano P
- Subjects
- Adenocarcinoma, Mucinous genetics, Adenocarcinoma, Mucinous pathology, Aged, Case-Control Studies, Colorectal Neoplasms genetics, Colorectal Neoplasms pathology, Colorectal Neoplasms, Hereditary Nonpolyposis genetics, Colorectal Neoplasms, Hereditary Nonpolyposis pathology, DNA, Neoplasm metabolism, Diagnosis, Differential, Electrophoresis, Capillary standards, Female, Humans, Immunohistochemistry standards, Male, Middle Aged, Polymerase Chain Reaction standards, Prognosis, Retrospective Studies, Tissue Embedding methods, Tissue Embedding standards, Tissue Fixation methods, Tissue Fixation standards, Adenocarcinoma, Mucinous diagnosis, Colorectal Neoplasms diagnosis, Colorectal Neoplasms, Hereditary Nonpolyposis diagnosis, DNA, Neoplasm genetics, Microsatellite Instability
- Abstract
Immunohistochemistry (IHC) and polymerase chain reaction (PCR) and fragment separation by capillary electrophoresis represent the current clinical laboratory standard for the evaluation of microsatellite instability (MSI) status. The importance of reporting MSI status in colorectal cancer is based on its potential for guiding treatment and as a prognostic indicator. It is also used to identify patients for Lynch syndrome testing. Our aim was to evaluate pre-analytical factors, such as age of formalin-fixed and paraffin-embedded (FFPE) block, neoplastic cell percentage, mucinous component, and DNA integrity, that may influence the accuracy of MSI testing and assess the concordance between three different MSI evaluation approaches. We selected the mucinous colorectal cancer (CRC) histotype for this study as it may possibly represent an intrinsic diagnostic issue due to its low tumor cellularity. Seventy-five cases of mucinous CRC and corresponding normal colon tissue samples were retrospectively selected. MMR proteins were evaluated by IHC. After DNA quality and quantity evaluation, the Idylla™ and TapeStation 4200 platforms were adopted for the evaluation of MSI status. Seventy-three (97.3%) cases were successfully analyzed by the three methodologies. Overall, the Idylla™ platform showed a concordance rate with IHC of 98.0% for microsatellite stable (MSS)/proficient MMR (pMMR) cases and 81.8% for MSI/deficient MMR (dMMR) cases. The TapeStation 4200 system showed a concordance rate with IHC of 96.0% for MSS/pMMR cases and 45.4% for MSI/dMMR cases. The concordance rates of the TapeStation 4200 system with respect to the Idylla™ platform were 98.1% for MSS profile and 57.8% for MSI profile. Discordant cases were analyzed using the Titano MSI kit. Considering pre-analytical factors, no significant variation in concordance rate among IHC analyses and molecular systems was observed by considering the presence of an acellular mucus cut-off >50% of the tumor area, FFPE year preparation, and DNA concentration. Conversely, the Idylla™ platform showed a significant variation in concordance rate with the IHC approach by considering a neoplastic cell percentage >50% ( p -value = 0.002), and the TapeStation 4200 system showed a significant variation in concordance rate with the IHC approach by considering a DNA integrity number (DIN) ≥4 as cut-off ( p -value = 0.009). Our data pinpoint a central role of the pre-analytical phase in the diagnostic outcome of MSI testing in CRC.
- Published
- 2020
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42. Primary effusion lymphoma metachronous to multicentric Castleman disease in an immunocompetent patient.
- Author
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Parente P, Zanelli M, Zizzo M, Covelli C, Carosi I, Ascani S, and Graziano P
- Subjects
- Castleman Disease pathology, Humans, Lymphoma, Primary Effusion pathology, Male, Middle Aged, Castleman Disease complications, Lymphoma, Primary Effusion complications
- Abstract
Human Herpesvirus 8 (HHV8) has been associated with a wide spectrum of B-cell lymphoproliferative disorders, including Primary Effusion Lymphoma, Multicentric Castleman Disease, HHV8-positive Diffuse Large B-cell Lymphoma, not otherwise specified and germinotropic lymphoproliferative disorder. The association of different HHV8-related lymphoproliferative disorders is described in immunodeficient patients. We report a case of Primary Effusion Lymphoma metachronous to Multicentric Castleman Disease in an immunocompetent patient., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier GmbH. All rights reserved.)
- Published
- 2020
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43. Intestinal adenosquamous carcinoma with a synchronous skin metastasis: a immunohistochemical and molecular analysis.
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Parente P, Covelli C, Parrella P, Latiano TP, Fiordelisi F, Pellico MT, Maiello E, and Graziano P
- Subjects
- Aged, Carcinoma, Adenosquamous chemistry, Carcinoma, Adenosquamous genetics, Colonic Neoplasms chemistry, Colonic Neoplasms genetics, Facial Neoplasms chemistry, Facial Neoplasms genetics, Humans, Male, Mutation, Missense, Predictive Value of Tests, Proto-Oncogene Proteins p21(ras) genetics, Skin Neoplasms chemistry, Skin Neoplasms genetics, beta Catenin analysis, beta Catenin genetics, Biomarkers, Tumor analysis, Biomarkers, Tumor genetics, Carcinoma, Adenosquamous secondary, Colonic Neoplasms pathology, DNA Mutational Analysis, Facial Neoplasms secondary, Immunohistochemistry, Skin Neoplasms secondary
- Abstract
Introduction: Intestinal adenosquamous carcinoma (ASC) is a rare colorectal neoplasm frequently occurring at onset as a locally advanced disease with distant metastases. The liver is the most common site of metastasis, followed by the peritoneum and the lung. Cutaneous metastases from usual colorectal adenocarcinoma occur in about 3% of cases, both at the time of diagnosis in advanced disease and during the follow-up. To the best of our knowledge, skin metastasis from ASC has never been described, and no biological landscape of ASC has ever been investigated., Methods: We report a case of synchronous intestinal ASC and cutaneous single facial metastasis in a 70-year-old man with morphological, immunohistochemical, and molecular analysis of primary and metastatic lesions., Results: Primary and metastatic ASC showed the same morphological and immunohistochemical features. Target sequencing analysis revealed, both in primary tumor and metastasis, a pathogenic KRAS gene missense mutation c.38G > A p.(Gly13Asp) and a likely pathogenic CTNNB1 gene missense mutation c.94G > A p.(Asp32Asn). A nuclear localization of β-catenin protein in adenocarcinomatous component of primary and metastatic lesions was observed on immunohistochemistry., Conclusion: We describe a case of single synchronous facial cutaneous metastasis from intestinal ASC showing KRAS and CTNN1B mutations both on primary and metastatic lesions.
- Published
- 2020
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44. Mismatch repair proteins and microsatellite instability in solid pseudopapillary neoplasm of the pancreas.
- Author
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Covelli C, Parente P, Pepe F, Pisapia P, Fiordelisi F, and Malapelle U
- Subjects
- Adult, DNA-Binding Proteins analysis, Female, Humans, Immunohistochemistry, Male, Middle Aged, Mismatch Repair Endonuclease PMS2 analysis, MutL Protein Homolog 1 analysis, MutS Homolog 2 Protein analysis, Neoplasm Grading, Pancreatic Neoplasms pathology, Polymerase Chain Reaction, Young Adult, Biomarkers, Tumor analysis, DNA Mismatch Repair, Microsatellite Instability, Pancreatic Neoplasms chemistry, Pancreatic Neoplasms genetics
- Published
- 2019
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45. Collagen proportionate area is an independent predictor of long-term outcome in patients with non-alcoholic fatty liver disease.
- Author
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Buzzetti E, Hall A, Ekstedt M, Manuguerra R, Guerrero Misas M, Covelli C, Leandro G, Luong T, Kechagias S, Manesis EK, Pinzani M, Dhillon AP, and Tsochatzis EA
- Subjects
- Adult, Aged, Biomarkers analysis, Biomarkers metabolism, Biopsy, Collagen analysis, Female, Follow-Up Studies, Greece epidemiology, Humans, Liver chemistry, Liver pathology, Liver Cirrhosis diagnosis, Liver Cirrhosis metabolism, Liver Cirrhosis mortality, Liver Cirrhosis therapy, Liver Transplantation, Longitudinal Studies, Male, Middle Aged, Non-alcoholic Fatty Liver Disease metabolism, Non-alcoholic Fatty Liver Disease mortality, Non-alcoholic Fatty Liver Disease therapy, Prognosis, Retrospective Studies, Sweden epidemiology, Treatment Outcome, United Kingdom epidemiology, Collagen metabolism, Liver metabolism, Non-alcoholic Fatty Liver Disease diagnosis
- Abstract
Background: Collagen proportionate area (CPA) measurement is a technique that quantifies fibrous tissue in liver biopsies by measuring the amount of collagen deposition as a proportion of the total biopsy area. CPA predicts clinical outcomes in patients with HCV and can sub-classify cirrhosis., Aim: To test the ability of CPA to quantify fibrosis and predict clinical outcomes in patients with NAFLD., Methods: We assessed consecutive patients with biopsy-proven NAFLD from three European centres. Clinical and laboratory data were collected at baseline and at the time of the last clinical follow-up or death. CPA was performed at two different objective magnifications, whole biopsy macro and ×4 objective magnification, named standard (SM) and high (HM) magnification respectively. The correlation between CPA and liver stiffness was assessed in a sub-group of patients., Results: Of 437 patients, 32 (7.3%) decompensated and/or died from liver-related causes during a median follow-up of 103 months. CPA correlated with liver stiffness and liver fibrosis stage across the whole spectrum of fibrosis. HM CPA was significantly higher than SM CPA in stages F0-F3 but similar in cirrhosis, reflecting a higher ability to capture pericellular/perisinusoidal fibrosis at early stages. Age at baseline (HR: 1.04, 95% CI: 1.01-1.08), HM CPA (HR: 1.04 per 1% increase, 95% CI: 1.01-1.08) and presence of advanced fibrosis (HR: 15.4, 95% CI: 5.02-47.84) were independent predictors of liver-related clinical outcomes at standard and competing risk multivariate Cox-regression analysis., Conclusions: CPA accurately measures fibrosis and is an independent predictor of clinical outcomes in NAFLD; hence it merits further evaluation as a surrogate endpoint in clinical trials., (© 2019 John Wiley & Sons Ltd.)
- Published
- 2019
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46. Vascular density and inflammatory infiltrate in primary oral squamous cell carcinoma and after allogeneic hematopoietic stem cell transplantation.
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Tamma R, Limongelli L, Maiorano E, Pastore D, Cascardi E, Tempesta A, Carluccio P, Mastropasqua MG, Capodiferro S, Covelli C, Pentenero M, Annese T, Favia G, Specchia G, and Ribatti D
- Subjects
- Adult, Aged, Aged, 80 and over, Allografts, Chronic Disease, Female, Humans, Male, Middle Aged, Retrospective Studies, Carcinoma, Squamous Cell blood supply, Carcinoma, Squamous Cell metabolism, Carcinoma, Squamous Cell pathology, Carcinoma, Squamous Cell therapy, Graft vs Host Disease metabolism, Graft vs Host Disease pathology, Graft vs Host Disease therapy, Hematopoietic Stem Cell Transplantation, Mouth Neoplasms blood supply, Mouth Neoplasms metabolism, Mouth Neoplasms pathology, Mouth Neoplasms therapy, Neovascularization, Pathologic metabolism, Neovascularization, Pathologic pathology, Neovascularization, Pathologic therapy
- Abstract
Hematopoietic stem cell transplantation (HSCT) recipients have been reported to have an increased risk of chronic graft versus host disease (cGVHD) and hematological and solid cancers. Oral manifestations are the first signs of cGVHD observed in the majority of patients, and oropharyngeal cancer is the most frequent secondary malignancy occurred after HSCT. In this study, we have evaluated the inflammatory infiltrate cell content and correlated with the vascular density in patients affected by primary oral squamous cell carcinoma (OSCC) from previous healthy controls and OSCC after cGVHD. Results have demonstrated that patients with OSCC after GVHD show a more consistent inflammatory infiltrate as compared with the OSCC ones. In detail, the inflammatory background composed of CD3-positive T cells, tryptase-positive mast cells, CD31-positive endothelial cells, and CD68-positive macrophages may be more pronounced in the setting of GVHD + OSCC than in the control group. By contrast, CD20-positive B cells and CD1a-positive dendritic cells were more abundant in the latter population. Finally, a positive correlation was found as between vascular density and inflammatory cell infiltration in both GVHD + OSCC and OSCC groups. Overall, these results confirm the role played by immune cells in enhancing tumor progression and angiogenesis and suggest a potential therapeutic strategy involving inhibition of recruitment of immune cells to the tumor microenvironment and blockade of pro-tumoral effects and pro-angiogenic functions.
- Published
- 2019
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47. Crosstalk between the Tumor Microenvironment and Immune System in Pancreatic Ductal Adenocarcinoma: Potential Targets for New Therapeutic Approaches.
- Author
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Parente P, Parcesepe P, Covelli C, Olivieri N, Remo A, Pancione M, Latiano TP, Graziano P, Maiello E, and Giordano G
- Abstract
Pancreatic ductal adenocarcinoma is a lethal disease for which radical surgery and chemotherapy represent the only curative options for a small proportion of patients. Recently, FOLFIRINOX and nab-paclitaxel plus gemcitabine have improved the survival of metastatic patients but prognosis remains poor. A pancreatic tumor microenvironment is a dynamic milieu of cellular and acellular elements, and it represents one of the major limitations to chemotherapy efficacy. The continued crosstalk between cancer cells and the surrounding microenvironment causes immunosuppression within pancreatic immune infiltrate increasing tumor aggressiveness. Several potential targets have been identified among tumor microenvironment components, and different therapeutic approaches are under investigation. In this article, we provide a qualitative literature review about the crosstalk between the tumor microenvironment components and immune system in pancreatic cancer. Finally, we discuss potential therapeutic strategies targeting the tumor microenvironment and we show the ongoing trials.
- Published
- 2018
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48. Mercury in the unconfined aquifer of the Isonzo/Soča River alluvial plain downstream from the Idrija mining area.
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Andrea C, Stefano C, Andrea E, Elena P, Elisa P, Asta G, Janko U, Enrico Z, and Luca Z
- Subjects
- Mining, Slovenia, Environmental Monitoring methods, Groundwater analysis, Mercury analysis, Mercury Compounds analysis, Rivers chemistry, Water Pollutants, Chemical analysis
- Abstract
This work aims at evaluating mercury (Hg) occurrence, spatial distribution and speciation in groundwater of the Isonzo/Soča River upper alluvial plain downstream from the Idrija Hg mine (Western Slovenia). Several wells and piezometers were sampled both in static and dynamic mode. Total (THg) and filtered (FHg) concentrations were generally higher in static (THg, 1.87-855 ng L
-1 ; FHg, 0.20-13.61 ng L-1 ) than in dynamic mode (THg, 0.08-78.77 ng L-1 ; FHg, 0.28-6.65 ng L-1 ). The estimated background value accounts for 2-3 ng L-1 . On the basis of hydrochemistry and isotopic composition, the main sources of groundwater were established. Hg concentrations in the Slovenian sector, supplied by local rainfall, are comparable to values measured close to the Isonzo River. Possible further Hg local sources have been suggested. Stability field analysis for the aqueous Hg species revealed that in the presence of chloride Hg solubility may be increased by the formation of chlorocomplexes. Mercury that rarely enters reduced surrounding conditions can be bound to sulphur to form polysulphide species depending on the pH of water. Since Hg-contaminated alluvial sediments of the Isonzo River may act as a secondary Hg source in groundwater, a borehole was dug down to the water table. Mercury content and speciation revealed that cinnabar (HgS) is the prevalent form followed by the matrix-bound Hg (Hgbound ). Variations of the physico-chemical boundary conditions, as well as the raising/lowering of the water table, may be locally responsible for the slight variability of Hg concentrations in the aquifer., (Copyright © 2017 Elsevier Ltd. All rights reserved.)- Published
- 2018
- Full Text
- View/download PDF
49. DRESS syndrome triple whammy: sulfasalazine, amoxicillin and HHV-7.
- Author
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Lang M, Fish J, Covelli C, and Schreiber BE
- Subjects
- Adult, Anti-Infective Agents adverse effects, Arthritis, Reactive complications, DNA, Viral analysis, Dose-Response Relationship, Drug, Drug Hypersensitivity Syndrome drug therapy, Earache complications, Female, Glucocorticoids administration & dosage, Humans, Roseolovirus Infections virology, Amoxicillin adverse effects, Arthritis, Reactive drug therapy, Drug Hypersensitivity Syndrome etiology, Earache drug therapy, Herpesvirus 7, Human genetics, Roseolovirus Infections complications, Sulfasalazine adverse effects
- Published
- 2017
- Full Text
- View/download PDF
50. Human microRNA expression in sporadic and FAP-associated desmoid tumors and correlation with beta-catenin mutations.
- Author
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Cavallini A, Rotelli MT, Lippolis C, Piscitelli D, Digennaro R, Covelli C, Carella N, Accetturo M, and Altomare DF
- Subjects
- Abdominal Neoplasms metabolism, Abdominal Neoplasms pathology, Adenomatous Polyposis Coli metabolism, Adenomatous Polyposis Coli pathology, Adolescent, Adult, Aged, DNA Mutational Analysis, Female, Fibromatosis, Aggressive metabolism, Fibromatosis, Aggressive pathology, Gene Expression Profiling, Genes, APC, Humans, Immunohistochemistry, Male, Middle Aged, RNA Interference, RNA, Messenger genetics, Serpins genetics, Tetraspanins genetics, Transcriptome, Tumor Burden, Young Adult, beta Catenin metabolism, Abdominal Neoplasms genetics, Adenomatous Polyposis Coli genetics, Fibromatosis, Aggressive genetics, Gene Expression Regulation, Neoplastic, MicroRNAs genetics, Mutation, beta Catenin genetics
- Abstract
Desmoid tumors (DT) are rare, benign, fibroblastic neoplasm with challenging histological diagnosis. DTs can occur sporadically or associated with the familial adenomatous polyposis coli (FAP). Most sporadic DTs are associated with β-catenin gene (CTNNB1) mutations, while mutated APC gene causes FAP disease. microRNAs (miRNAs) are involved in many human carcinogenesis.The miRNA profile was analyzed by microarray in formalin-fixed, paraffin-embedded (FFPE) specimens of 12 patients (8 sporadic, 4 FAP-associated) and 4 healthy controls. One hundred and one mRNAs resulted dysregulated, of which 98 in sporadic DTs and 8 in FAP-associated DTs, 5 were shared by both tumors. Twenty-six miRNAs were then validated by RT-qPCR in 23 sporadic and 7 FAP-associated DT samples matched with healthy controls. The qPCR method was also used to evaluate the CTNNB1 mutational status in sporadic DTs. The correlation between sporadic DTs and miRNA expression showed that miR-21-3p increased in mutated versus wild-type DTs, while miR-197-3p was decreased. The mRNA expression of Tetraspanin3 and Serpin family A member 3, as miR-21-3p targets, and L1 Cell Adhesion Molecule, as miR-197-3p target, was also evaluate. CTNNB1 mutations associated to miRNA dysregulation could affect the genesis and the progression of this disease and help histological diagnosis of sporadic DTs.
- Published
- 2017
- Full Text
- View/download PDF
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