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1. L'acétylcholinestérase des poissons, cible des organophosphorés et des carbamates. Caractérisation du gène et des formes moléculaires de l'enzyme chez Danio rerio. Effets des anticholinestérasiques

Author

BERTRAND C., COUSIN X., HAUBRUGE E., TOUTANT J. P., and CHATONNET A.

Subjects
Aquaculture. Fisheries. Angling, SH1-691
Abstract

L'acétylcholinestérase (AChE, EC 3.1.1.7) est une enzyme indispensable de la transmission synaptique cholinergique. Son inhibition par les organophosphorés et les carbamates est à la base de certains traitements insecticides et nématicides utilisés en agriculture. Les faunes sauvage (en particulier les poissons) et domestique sont exposées aux résidus des traitements anticholinestérasiques. La mesure de l'inhibition de l'activité AChE chez ces espèces est un marqueur fiable de l'exposition. Ceci est montré ici pour l'AChE de carpes exposées expérimentalement au carbofuran (carbamate). Nous présentons parallèlement des données sur le gène de l'AChE chez Danio rerio et sur les formes moléculaires de l'enzyme chez Danio, Cyprinus carpio et Oncorhynchus mykiss. Contrairement à ce qui est observé chez d'autres vertébrés, le gène d'AChE de Danio ne possède pas d'exon H et seules les sous-unités de type T ont pu être identifiées chez ces trois espèces. Des résultats préliminaires d'hybridation in situ sur les embryons sont présentés et nous discutons l'intérêt des expériences de transgenèse dans l'oeuf de Danio pour étudier d'éventuelles fonctions morphogénétiques de l'AChE au début du développement.

Published
1998
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3. The time for ambitious action is now: Science-based recommendations for plastic chemicals to inform an effective global plastic treaty

Author

Brander, S.M., Senathirajah, K., Fernandez, M.O., Weis, J.S., Kumar, E., Jahnke, Annika, Hartmann, N.B., Alava, J.J., Farrelly, T., Carney Almroth, B., Groh, K.J., Syberg, K., Buerkert, J.S., Abeynayaka, A., Booth, A.M., Cousin, X., Herzke, D., Monclús, L., Morales-Caselles, C., Bonisoli-Alquati, A., Al-jaibachi, R., Wagner, M., Brander, S.M., Senathirajah, K., Fernandez, M.O., Weis, J.S., Kumar, E., Jahnke, Annika, Hartmann, N.B., Alava, J.J., Farrelly, T., Carney Almroth, B., Groh, K.J., Syberg, K., Buerkert, J.S., Abeynayaka, A., Booth, A.M., Cousin, X., Herzke, D., Monclús, L., Morales-Caselles, C., Bonisoli-Alquati, A., Al-jaibachi, R., and Wagner, M.

Abstract

The ubiquitous and global ecological footprint arising from the rapidly increasing rates of plastic production, use, and release into the environment is an important modern environmental issue. Of increasing concern are the risks associated with at least 16,000 chemicals present in plastics, some of which are known to be toxic, and which may leach out both during use and once exposed to environmental conditions, leading to environmental and human exposure. In response, the United Nations member states agreed to establish an international legally binding instrument on plastic pollution, the global plastics treaty. The resolution acknowledges that the treaty should prevent plastic pollution and its related impacts, that effective prevention requires consideration of the transboundary nature of plastic production, use and pollution, and that the full life cycle of plastics must be addressed. As a group of scientific experts and members of the Scientists' Coalition for an Effective Plastics Treaty, we concur that there are six essential “pillars” necessary to truly reduce plastic pollution and allow for chemical detoxification across the full life cycle of plastics. These include a plastic chemical reduction and simplification, safe and sustainable design of plastic chemicals, incentives for change, holistic approaches for alternatives, just transition and equitable interventions, and centering human rights. There is a critical need for scientifically informed and globally harmonized information, transparency, and traceability criteria to protect the environment and public health. The right to a clean, healthy, and sustainable environment must be upheld, and thus it is crucial that scientists, industry, and policy makers work in concert to create a future free from hazardous plastic contamination.

Published
2024

4. List of Contributors

Author

Abbas, B., primary, Abreu, A., additional, Adams, R., additional, Adolfsson-Erici, M., additional, Afonso, A., additional, Afonso-Olivares, C., additional, Agirbas, E., additional, Aguiló, J.M., additional, Airoldi, L., additional, Aksoy, H., additional, Albentosa, M., additional, Alcaro, L., additional, Aliani, S., additional, Al-Maslamani, I., additional, Alomar, C., additional, Altin, D., additional, Álvarez, E., additional, Amaral-Zettler, L.A., additional, Amato, E., additional, Anderson, A., additional, Andrady, A.L., additional, Andrius, G., additional, Angel, D., additional, Ariese, F., additional, Arp, H.P., additional, Asensio, M., additional, Assidqi, K., additional, Avio, C.G., additional, Aytan, U., additional, Bahri, T., additional, Baini, M., additional, Bakir, A., additional, Ball, H., additional, Baranyi, C., additional, Barboza, L.G.A., additional, Barg, U., additional, Bargelloni, L., additional, Barras, H., additional, Barrera, C., additional, Barria, P., additional, Barrows, A., additional, Barth, A., additional, Batel, A., additional, Baztan, J., additional, Baztan, P., additional, Beiras, R., additional, Benedetti, M., additional, Berber, A.A., additional, Berber, N., additional, Bergmann, M., additional, Berlino, M., additional, Berrow, S., additional, Bessa, F., additional, Besseling, E., additional, Beyer, B., additional, Binaglia, M., additional, Bizjak, T., additional, Bjorndal, K.A., additional, Blust, R., additional, Boertien, M., additional, Bolten, A.B., additional, Booth, A.M., additional, Bounoua, B., additional, Bourseau, P., additional, Brahimi, N., additional, Bramini, M., additional, Brennholt, N., additional, Breuninger, E., additional, Bried, J., additional, Broderick, A., additional, Broglio, E., additional, Browne, M.A., additional, Bruzaud, S., additional, Buceta, J., additional, Buchinger, S., additional, Budimir, S., additional, Budzin-ski, H., additional, Butter, E., additional, Cachot, J., additional, Caetano, M., additional, Callaghan, A., additional, Camedda, A., additional, Capella, S., additional, Cardelli, L., additional, Carpentieri, S., additional, Carrasco, A., additional, Carriço, R., additional, Caruso, A., additional, Cassone, A.-L., additional, Castillo, A., additional, Castro, R.O., additional, Catarino, A.I., additional, Cazenave, P.W., additional, Çelik, İ., additional, Cerralbo, P., additional, César, G., additional, Chouinard, O., additional, Chubarenko, I., additional, Chubarenko, I.P., additional, Cicero, A.M., additional, Clarindo, G., additional, Clarke, B., additional, Clérandeau, C., additional, Clüsener-Godt, M., additional, Codina-García, M., additional, Cole, M., additional, Collard, F., additional, Collignon, A., additional, Collins, T., additional, Compa, M., additional, Conan, P., additional, Constant, M., additional, Cordier, M., additional, Courtene-Jones, W., additional, Cousin, X., additional, Covelo, P., additional, Cózar, A., additional, Crichton, E., additional, Crispi, O., additional, Cronin, M., additional, Croot, P.L., additional, Cruz, M.J., additional, d’Errico, G., additional, Dâmaso, C., additional, Das, K., additional, de Alencastro, L.F., additional, de Araujo, F.V., additional, de Boer, J.F., additional, de Lucia, G.A., additional, Debeljak, P., additional, Dehaut, A., additional, Deudero, S., additional, Devrieses, L., additional, Di Vito, S., additional, Díaz, A., additional, Donohue, J., additional, Doumenq, P., additional, Doyle, T.K., additional, Dris, R., additional, Druon, J.-N., additional, Duarte, C.M., additional, Duflos, G., additional, Dumontier, M., additional, Duncan, E., additional, Dussud, C., additional, Eckerlebe, A., additional, Egelkraut-Holtus, M., additional, Eidsvoll, D.P., additional, Ek, C., additional, Elena, S., additional, Elineau, A., additional, Enevoldsen, H., additional, Eppe, G., additional, Eriksen, M., additional, Ernsteins, R., additional, Espino, M., additional, Estévez-Calvar, N., additional, Ewins, C., additional, Fabre, P., additional, Faimali, M., additional, Fattorini, D., additional, Faure, F., additional, Ferrando, S., additional, Ferreira, J.C., additional, Ferreira-da-Costa, M., additional, Fileman, E., additional, Fischer, M., additional, Fortunato, A.B., additional, Fossi, M.C., additional, Foulon, V., additional, Frank, A., additional, Frenzel, M., additional, Frère, L., additional, Frias, J.P.G.L., additional, Frick, H., additional, Froneman, P.W., additional, Gabet, V.M., additional, Gabrielsen, G.W., additional, Gago, J., additional, Gajst, T., additional, Galgani, F., additional, Gallinari, M., additional, Galloway, T.S., additional, Gamarro, E.G., additional, Gambardella, C., additional, Garaventa, F., additional, Garcia, S., additional, Garrabou, J., additional, Garrido, P., additional, Gary, S.F., additional, Gasperi, J., additional, Gaze, W., additional, Geertz, T., additional, Gelado-Caballero, M.D., additional, George, M., additional, Gercken, J., additional, Gerdts, G., additional, Ghiglione, J.-F., additional, Gies, E., additional, Gilbert, B., additional, Giménez, L., additional, Glassom, D., additional, Glockzin, M., additional, Godley, B., additional, Goede, K., additional, Goksøyr, A., additional, Gómez, M., additional, Gómez-Parra, A., additional, González-Marco, D., additional, González-Solís, J., additional, Gorbi, S., additional, Gorokhova, E., additional, Gorsky, G., additional, Gosch, M., additional, Grose, J., additional, Guebitz, G.M., additional, Guedes-Alonso, R., additional, Guijarro, B., additional, Guilhermino, L., additional, Gundry, T., additional, Gutow, L., additional, Haave, M., additional, Haeckel, M., additional, Haernvall, K., additional, Hajbane, S., additional, Hamann, M., additional, Hämer, J., additional, Hamm, T., additional, Hansen, B.H., additional, Hardesty, B.D., additional, Harth, B., additional, Hartikainen, S., additional, Hassellöv, M., additional, Hatzky, S., additional, Healy, M.G., additional, Hégaret, H., additional, Henry, T.B., additional, Hermabessiere, L., additional, Hernández-Brito, J.J., additional, Hernandez-Gonzalez, A., additional, Hernandez-Milian, G., additional, Hernd, G., additional, Herrera, A., additional, Herring, C., additional, Herzke, D., additional, Heussner, S., additional, Hidalgo-Ruz, V., additional, Himber, C., additional, Holland, M., additional, Hong, N.-H., additional, Horton, A.A., additional, Horvat, P., additional, Huck, T., additional, Huhn, M., additional, Huvet, A., additional, Iglesias, M., additional, Igor, C., additional, Isachenko, I.A., additional, Ivar do Sul, J-A., additional, Jahnke, A., additional, Janis, B., additional, Janis, K., additional, Janis, U., additional, Jemec, A., additional, Jiménez, J.C., additional, Johnsen, H., additional, Jorgensen, B., additional, Jørgensen, J.H., additional, Jörundsdóttir, H., additional, Jung, Y.-J., additional, Kedzierski, M., additional, Keiter, S., additional, Kershaw, P., additional, Kerhervé, P., additional, Kesy, K., additional, Khan, F., additional, Khatmullina, L.I., additional, Kirby, J., additional, Kiriakoulakis, K., additional, Klein, R., additional, Klunderud, T., additional, Knudsen, C.M.H., additional, Knudsen, T.B., additional, Kochleus, C., additional, Koelmans, A.A., additional, Kögel, T., additional, Koistinen, A., additional, Kopke, K., additional, Korez, Š., additional, Kowalski, N., additional, Kreikemeyer, B., additional, Kroon, F., additional, Krumpen, T., additional, Krzan, A., additional, Kržan, A., additional, Labrenz, M., additional, Lacroix, C., additional, Ladirat, L., additional, Laforsch, C., additional, Lagarde, F., additional, Lahive, E., additional, Lambert, C., additional, Lapucci, C., additional, Lattin, G., additional, Law, K.L., additional, Le Roux, F., additional, Le Souef, K., additional, Le Tilly, V., additional, Lebreton, L., additional, Leemans, E., additional, Lehtiniemi, M., additional, Lenz, M., additional, Leskinen, J., additional, Leslie, H., additional, Leslie, H.A., additional, Levasseur, C., additional, Lewis, C., additional, Licandro, P., additional, Lind, K., additional, Lindeque, P., additional, Lindeque, P.K., additional, Lips, I., additional, Liria, A., additional, Liria-Loza, A., additional, Llinás, O., additional, Loiselle, S.A., additional, Long, M., additional, Lorenz, C., additional, Lorenzo, S.M., additional, Loubar, K., additional, Luna-Jorquera, G., additional, Lusher, A.L., additional, Macchia, V., additional, MacGabban, S., additional, Mackay, K., additional, MacLeod, M., additional, Maes, T., additional, Magaletti, E., additional, Maggiore, A., additional, Magnusson, K., additional, Mahon, A.M., additional, Makorič, P., additional, Mallow, O., additional, Marques, J., additional, Marsili, L., additional, Martí, E., additional, Martignac, M., additional, Martin, J., additional, Martínez, I., additional, Martínez, J., additional, Martinez-Gil, M., additional, Martins, H.R., additional, Matiddi, M., additional, Maximenko, N., additional, Mazlum, R., additional, Mcadam, R., additional, Mcknight, L., additional, McNeal, A.W., additional, Measures, J., additional, Mederos, M.S., additional, Mendoza, J., additional, Meyer, M.S., additional, Miguelez, A., additional, Milan, M., additional, Militão, T., additional, Miller, R.Z., additional, Mino-Vercellio-Verollet, M., additional, Mir, G., additional, Miranda-Urbina, D., additional, Misurale, F., additional, Montesdeoca-Esponda, S., additional, Mora, J., additional, Morgana, S., additional, Moriceau, B., additional, Morin, B., additional, Morley, A., additional, Morrison, L., additional, Murphy, F., additional, Naidoo, T., additional, Näkki, P., additional, Napper, I.E., additional, Narayanaswamy, B.E., additional, Nash, R., additional, Negri, A., additional, Nel, H.A., additional, Nerheim, M.S., additional, Nerland, I.L., additional, Neto, J., additional, Neves, V., additional, Nies, H., additional, Noel, M., additional, Nor, N.H.M., additional, Noren, F., additional, O’ Connell, B., additional, O’ Connor, I., additional, Obbard, J.P., additional, Oberbeckmann, S., additional, Obispo, R., additional, Officer, R., additional, Ogonowski, M., additional, Orbea, A., additional, Ortlieb, M., additional, Osborn, A.M., additional, Ostiategui-Francia, P., additional, Packard, T., additional, Pahl, S., additional, Palatinus, A., additional, Palmqvist, A., additional, Pannetier, P., additional, Panti, C., additional, Parmentier, E., additional, Pasanen, P., additional, Patarnello, T., additional, Pattiaratchi, C., additional, Pauletto, M., additional, Paulus, M., additional, Pavlekovsky, K., additional, Pedersen, H.B., additional, Pedrotti, M.-L., additional, Peeken, I., additional, Peeters, D., additional, Peeters, E., additional, Pellegrini, D., additional, Perales, J.A., additional, Perez, E., additional, Perz, V., additional, Petit, S., additional, Pflieger, M., additional, Pham, C.K., additional, Piazza, V., additional, Pinto, M., additional, Planells, O., additional, Plaza, M., additional, Pompini, O., additional, Potthoff, A., additional, Prades, L., additional, Primpke, S., additional, Proietti, M., additional, Proskurowski, G., additional, Puig, C., additional, Pujo-Pay, M., additional, Pullerits, K., additional, Queirós, A.M., additional, Quinn, B., additional, Raimonds, E., additional, Ramis-Pujol, J., additional, Rascher-Friesenhausen, R., additional, Reardon, E., additional, Regoli, F., additional, Reichardt, A.M., additional, Reifferscheid, G., additional, Reilly, K., additional, Reisser, J., additional, Riba, I., additional, Ribitsch, D., additional, Rinnert, E., additional, Rios, N., additional, Rist, S.E., additional, Rivadeneira, M.M., additional, Rivière, G., additional, Robbens, J., additional, Robertson, C.J.R., additional, Rocher, V., additional, Rochman, C.M., additional, Rodrigues, M., additional, Rodriguez, Y., additional, Rodríguez, A., additional, Rodríguez, G., additional, Rodríguez, J.R.B., additional, Rodríguez, S., additional, Rodríguez, Y., additional, Rogan, E., additional, Rojo-Nieto, E., additional, Romeo, T., additional, Ross, P.S., additional, Roveta, A., additional, Rowland, S.J., additional, Ruckstuhl, N.A., additional, Ruiz-Fernández, A-C., additional, Ruiz-Orejón, L.F., additional, Runge, J., additional, Russell, M., additional, Saavedra, C., additional, Saborowski, R., additional, Sahin, B.E., additional, Sailley, S., additional, Sakaguchi-Söder, K., additional, Salaverria, I., additional, Sánchez-Arcilla, A., additional, Sánchez-Nieva, J., additional, Sanderson, W., additional, Santana-Rodríguez, J.J., additional, Santana-Viera, S., additional, Santos, M.B., additional, Santos, M.R., additional, Sanz, M.R., additional, Sardá, R., additional, Savelli, H., additional, Schoeneich-Argent, R., additional, Scholz-Böttcher, B.M., additional, Sciacca, F., additional, Scofield, R.P., additional, Setälä, O., additional, Selenius, M., additional, Sempere, R., additional, Senturk, Y., additional, Shashoua, Y., additional, Sherman, P., additional, Sick, C., additional, Siegel, D., additional, Sierra, J.P., additional, Silva, F., additional, Silvestri, C., additional, Sintija, G., additional, Sire, O., additional, Slat, B., additional, Smit, A., additional, Sobral, P., additional, Sorvari, J., additional, Sosa-Ferrera, Z., additional, Sotillo, M.G., additional, Soudant, P., additional, Speidel, L., additional, Spurgeon, D.J., additional, Steer, M.K., additional, Steindal, C.C., additional, Stifanese, R., additional, Štindlová, A., additional, Stuurman, L., additional, Suaria, G., additional, Suazo, C.G., additional, Sureda, A., additional, Surette, C., additional, Svendsen, C., additional, Syberg, K., additional, Tairova, Z., additional, Talvitie, J., additional, Tassin, B., additional, Tazerout, M., additional, Tekman, M.B., additional, ter Halle, A., additional, Thiel, M., additional, Thomas, K.V., additional, Thompson, R.C., additional, Tinkara, T., additional, Tirelli, V., additional, Tomassetti, P., additional, Toorman, E., additional, Toppe, J., additional, Tornambè, A., additional, Torres, R., additional, Torres-Padrón, M.E., additional, Underwood, A.J., additional, Urbina, M., additional, Usategui-Martín, A., additional, Usta, R., additional, Valdés, L., additional, Valente, A., additional, Valentina, T., additional, van Arkel, K., additional, Van Colen, C., additional, Van Der Hal, N., additional, van Franeker, J.A., additional, Van Herwerden, L., additional, Van Loosdrecht, M., additional, van Oyen, A., additional, Vandeperre, F., additional, Vanderlinden, J-P., additional, Vani, D., additional, Vasconcelos, L., additional, Vega-Moreno, D., additional, Ventero, A., additional, Vethaak, A.D., additional, Vianello, A., additional, Vicioso, M., additional, Vieira, L.R., additional, Viršek, M.K., additional, Vos, M., additional, Wahl, M., additional, Wallace, N., additional, Walton, A., additional, Waniek, J.J., additional, Watts, A., additional, Webster, L., additional, Wesch, C., additional, Whitfield, E., additional, Wichels, A., additional, Wieczorek, A.M., additional, Wilcox, C., additional, Williams, R.J., additional, Wong-Wah-Chung, P., additional, Wright, S., additional, Wyles, K.J., additional, Young, R., additional, Yurtsever, M., additional, Yurtsever, U., additional, Zada, L., additional, Zamani, N.P., additional, and Zampetti, G., additional

Published
2017
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6. Can we combine genetics and two-step temperature control to move towards monosex European sea bass Dicentrarchus labrax?

Author

Vandeputte, Marc, Clota, Frederic, Vergnet, A., Blanc, Marie-Odile, Lallement, S., Ruelle, François, Sánchez Baizán, Núria, Besson, Mathieu, Piferrer, Francesc, Cousin, X., Goikoetxea, Alexander, Leitwein, M., Allal, François, Geffroy, Benjamin, Vandeputte, Marc, Clota, Frederic, Vergnet, A., Blanc, Marie-Odile, Lallement, S., Ruelle, François, Sánchez Baizán, Núria, Besson, Mathieu, Piferrer, Francesc, Cousin, X., Goikoetxea, Alexander, Leitwein, M., Allal, François, and Geffroy, Benjamin

Abstract

Introduction: The sea bass, Dicentrarchus labrax, is an important species for European mariculture, mainly in Mediterranean countries. In this species, females grow faster and reach a higher weight than males. However, most farmed fish batches show a highly male-skewed sex-ratio. The sea bass has a complex sex determination system, combining both genetic (polygenic) and environmental (temperature) influences. Low initial temperatures (<17°C) favor female sex determination. In contrast, temperature in the late post-larval phase has the opposite effect, with cold temperature favoring male differentiation and high temperature (up to 23°C) favoring female differentiation (Clota et al., 2021). However, data on the combined effects of genotype and temperature are lacking. We need to know whether there is genotype-by-environment (GxE) interaction or not, to deploy a strategy towards producing monosex female individuals for production while keeping a balanced sex-ratio for hatchery and selective breeding. Results: Starting at 1 day and using incremental rearing periods at 16°C (31 to 244 days), followed by an increase to 21°C, we first showed that, while 31 days at 16°C led to 26% of females, increased cold exposure promoted the proportion of females, up to 46% after 74 days at 16°C. Conversely, exposure to 16°C for a period longer than 74 days progressively reduced the proportion of females, reaching a minimum of ≈ 10% after 230 days at 16°C. In a second experiment, we demonstrated that after an initial rearing of fish for 90 days at 16°C, a secondary exposure to four different temperatures (19, 21, 23, 25°C) resulted in different sex-ratios, with more females at higher temperatures (from 30% at 19°C to 49% at 25°C). Then, we examined the genotype-by-environment interaction in both periods, by genotyping fish with a 57K SNP chip, comparing 1) two groups reared at 16 or 21°C during the first 60 days and 2) four groups of fish reared at 19, 21, 23 or 25°C after 90 days at 16

Published
2022

8. Organic contaminants sorbed to microplastics affect marine medaka fish early life stages development

Author

Le Bihanic, F., Clérandeau, C., Cormier, Bettie, Crebassa, J.-C., Keiter, Steffen, Beiras, R., Morin, B., Bégout, M.-L., Cousin, X., Cachot, J., Le Bihanic, F., Clérandeau, C., Cormier, Bettie, Crebassa, J.-C., Keiter, Steffen, Beiras, R., Morin, B., Bégout, M.-L., Cousin, X., and Cachot, J.

Abstract

The role of polyethylene microplastics 4–6 μm size (MPs) in the toxicity of environmental compounds to fish early life stages (ELS) was investigated. Marine medaka Oryzias melastigma embryos and larvae were exposed to suspended MPs spiked with three model contaminants: benzo(a)pyrene (MP-BaP), perfluorooctanesulfonic acid (MP-PFOS) and benzophenone-3 (MP-BP3) for 12 days. There was no evidence of MPs ingestion but MPs agglomerated on the surface of the chorion. Fish ELS exposed to virgin MPs did not show toxic effects. Exposure to MP-PFOS decreased embryonic survival and prevented hatching. Larvae exposed to MP-BaP or MP-BP3 exhibited reduced growth, increased developmental anomalies and abnormal behavior. Compared to equivalent waterborne concentrations, BaP and PFOS appeared to be more embryotoxic when spiked on MPs than when alone in seawater. These results suggest a relevant pollutant transfer by direct contact of MPs to fish ELS that should be included in the ecotoxicological risk assessment of MPs., Funding Agency:JPI Oceans program, as part of the EPHEMARE research project ANR-15-JOCE-0002

Published
2020
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9. Insights on Ecotoxicological Effects of Microplastics in Marine Ecosystems: The EPHEMARE Project.

Author

Cocca, M. (Maria Cristina), Di Pace, E., Errico, M.E. (Maria Emanuela), Gentile, G. (Gennaro), Montarsolo, A., Mossotti, R. (Raffaella), Avella, M., Regoli, Francesco, Albentosa, Marina, Avio, C.G., Batel, A., Bebianno, María Joao, Begout, M.L., Beiras, Ricardo, Bellas, Juan, Blust, R., Bour, A., Braunbeck, T., Cachot, Jérome, Catarci Carteny, C., Cormier, B., Cousin, X., Cuesta, Alberto, Esteban, María Ángeles, Faimali, M., Gambardella, C., Garaventa, F., Gorbi, S., Guilhermino, Lucía, Hylland, Ketil, Keiter, S.H., Kopke, K., Morin, B., Pacheco, A., Pittura, L., Town, R.M., Vieira, L.R., Cocca, M. (Maria Cristina), Di Pace, E., Errico, M.E. (Maria Emanuela), Gentile, G. (Gennaro), Montarsolo, A., Mossotti, R. (Raffaella), Avella, M., Regoli, Francesco, Albentosa, Marina, Avio, C.G., Batel, A., Bebianno, María Joao, Begout, M.L., Beiras, Ricardo, Bellas, Juan, Blust, R., Bour, A., Braunbeck, T., Cachot, Jérome, Catarci Carteny, C., Cormier, B., Cousin, X., Cuesta, Alberto, Esteban, María Ángeles, Faimali, M., Gambardella, C., Garaventa, F., Gorbi, S., Guilhermino, Lucía, Hylland, Ketil, Keiter, S.H., Kopke, K., Morin, B., Pacheco, A., Pittura, L., Town, R.M., and Vieira, L.R.

Published
2020

10. Multi- and transgenerational effects following early-life exposure of zebrafish to permethrin and coumarin 47: Impact on growth, fertility, behavior and lipid metabolism

Author

Blanc, M., Cormier, B., Hyötyläinen, T., Krauss, Martin, Scherbak, N., Cousin, X., Keiter, S.H., Blanc, M., Cormier, B., Hyötyläinen, T., Krauss, Martin, Scherbak, N., Cousin, X., and Keiter, S.H.

Abstract

Transgenerational effects induced by environmental stressors are a threat to ecosystems and human health. However, there is still limited observation and understanding of the potential of chemicals to influence life outcomes over several generations. In the present study, we investigated the effects of two environmental contaminants, coumarin 47 and permethrin, on exposed zebrafish (F0) and their progeny (F1–F3). Coumarin 47 is commonly found in personal care products and dyes, whereas permethrin is used as a domestic and agricultural pyrethroid insecticide/insect repellent. Zebrafish (F0) were exposed during early development until 28 days post-fertilization and their progeny (F1–F3) were bred unexposed. On one hand, the effects induced by coumarin 47 suggest no multigenerational toxicity. On the other hand, we found that behavior of zebrafish larvae was significantly affected by exposure to permethrin in F1 to F3 generations with some differences depending on the concentration. This suggests persistent alteration of the neural or neuromuscular function. In addition, lipidomic analyses showed that permethrin treatment was partially correlated with lysophosphatidylcholine levels in zebrafish, an important lipid for neurodevelopment. Overall, these results stress out one of the most widely used pyrethroids can trigger long-term, multi- and possibly transgenerational changes in the nervous system of zebrafish. These neurobehavioral changes echo the effects observed under direct exposure to high concentrations of permethrin and therefore call for more research on mechanisms underlying effect inheritance.

Published
2020

11. An ecotoxicological view on neurotoxicity assessment

Author

Legradi, J. B., Di Paolo, C., Kraak, M. H. S., van der Geest, H. G., Schymanski, E. L., Williams, A. J., Dingemans, M. M. L., Massei, R., Brack, W., Cousin, X., Begout, M. -L., van der Oost, R., Carion, A., Suarez-Ulloa, V., Silvestre, F., Escher, B. I., Engwall, M., Nilen, G., Keiter, S. H., Pollet, D., Waldmann, P., Kienle, C., Werner, I., Haigis, A. -C., Knapen, D., Vergauwen, L., Spehr, M., Schulz, W., Busch, W., Leuthold, D., Scholz, S., vom Berg, C. M., Basu, N., Murphy, C. A., Lampert, A., Kuckelkorn, J., Grummt, T., Hollert, H., Legradi, J. B., Di Paolo, C., Kraak, M. H. S., van der Geest, H. G., Schymanski, E. L., Williams, A. J., Dingemans, M. M. L., Massei, R., Brack, W., Cousin, X., Begout, M. -L., van der Oost, R., Carion, A., Suarez-Ulloa, V., Silvestre, F., Escher, B. I., Engwall, M., Nilen, G., Keiter, S. H., Pollet, D., Waldmann, P., Kienle, C., Werner, I., Haigis, A. -C., Knapen, D., Vergauwen, L., Spehr, M., Schulz, W., Busch, W., Leuthold, D., Scholz, S., vom Berg, C. M., Basu, N., Murphy, C. A., Lampert, A., Kuckelkorn, J., Grummt, T., and Hollert, H.

Abstract

The numbers of potential neurotoxicants in the environment are raising and pose a great risk for humans and the environment. Currently neurotoxicity assessment is mostly performed to predict and prevent harm to human populations. Despite all the efforts invested in the last years in developing novel in vitro or in silico test systems, in vivo tests with rodents are still the only accepted test for neurotoxicity risk assessment in Europe. Despite an increasing number of reports of species showing altered behaviour, neurotoxicity assessment for species in the environment is not required and therefore mostly not performed. Considering the increasing numbers of environmental contaminants with potential neurotoxic potential, eco-neurotoxicity should be also considered in risk assessment. In order to do so novel test systems are needed that can cope with species differences within ecosystems. In the field, online-biomonitoring systems using behavioural information could be used to detect neurotoxic effects and effect-directed analyses could be applied to identify the neurotoxicants causing the effect. Additionally, toxic pressure calculations in combination with mixture modelling could use environmental chemical monitoring data to predict adverse effects and prioritize pollutants for laboratory testing. Cheminformatics based on computational toxicological data from in vitro and in vivo studies could help to identify potential neurotoxicants. An array of in vitro assays covering different modes of action could be applied to screen compounds for neurotoxicity. The selection of in vitro assays could be guided by AOPs relevant for eco-neurotoxicity. In order to be able to perform risk assessment for eco-neurotoxicity, methods need to focus on the most sensitive species in an ecosystem. A test battery using species from different trophic levels might be the best approach. To implement eco-neurotoxicity assessment into European risk assessment, cheminformatics and in vitro scre

Published
2019
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12. Ecotoxicological effects of microplastics in marine ecosystems: insights from the EPHEMARE project

Author

Albentosa, Marina, Avio, C.G., Batel, A., Bebianno, M., Begout, M.L., Beiras, Ricardo, Bellas, Juan, Blust, R., Braunbeck, T., Cachot, Jérome, Carteny, C.C., Cormier, B., Cousin, X., Cuesta, Alberto, Esteban, María Ángeles, Faimali, M., Garaventa, F., Gorbi, S., Guilhermino, Lucía, Hylland, Ketil, Keiter, S., Kopke, K., Morin, B., Pittura, L., Regoli, Francesco, Town, R.M., Albentosa, Marina, Avio, C.G., Batel, A., Bebianno, M., Begout, M.L., Beiras, Ricardo, Bellas, Juan, Blust, R., Braunbeck, T., Cachot, Jérome, Carteny, C.C., Cormier, B., Cousin, X., Cuesta, Alberto, Esteban, María Ángeles, Faimali, M., Garaventa, F., Gorbi, S., Guilhermino, Lucía, Hylland, Ketil, Keiter, S., Kopke, K., Morin, B., Pittura, L., Regoli, Francesco, and Town, R.M.

Published
2019

14. An ecotoxicological view on neurotoxicity assessment

Author

Legradi, J.B., Di Paolo, C., Kraak, M.H.S., van der Geest, H.G., Schymanski, E.L., Williams, A.J., Dingemans, M.M.L., Massei, Riccardo, Brack, Werner, Cousin, X., Begout, M.-L., van der Oost, R., Carion, A., Suarez‑Ulloa, V., Silvestre, F., Escher, Beate, Engwall, M., Nilén, G., Keiter, S.H., Pollet, D., Waldmann, P., Kienle, C., Werner, I., Haigis, A.-C., Knapen, D., Vergauwen, L., Spehr, M., Schulz, W., Busch, Wibke, Leuthold, David, Scholz, Stefan, vom Berg, C.M., Basu, N., Murphy, C.A., Lampert, A., Kuckelkorn, J., Grummt, T., Hollert, H., Legradi, J.B., Di Paolo, C., Kraak, M.H.S., van der Geest, H.G., Schymanski, E.L., Williams, A.J., Dingemans, M.M.L., Massei, Riccardo, Brack, Werner, Cousin, X., Begout, M.-L., van der Oost, R., Carion, A., Suarez‑Ulloa, V., Silvestre, F., Escher, Beate, Engwall, M., Nilén, G., Keiter, S.H., Pollet, D., Waldmann, P., Kienle, C., Werner, I., Haigis, A.-C., Knapen, D., Vergauwen, L., Spehr, M., Schulz, W., Busch, Wibke, Leuthold, David, Scholz, Stefan, vom Berg, C.M., Basu, N., Murphy, C.A., Lampert, A., Kuckelkorn, J., Grummt, T., and Hollert, H.

Abstract

The numbers of potential neurotoxicants in the environment are raising and pose a great risk for humans and the environment. Currently neurotoxicity assessment is mostly performed to predict and prevent harm to human populations. Despite all the efforts invested in the last years in developing novel in vitro or in silico test systems, in vivo tests with rodents are still the only accepted test for neurotoxicity risk assessment in Europe. Despite an increasing number of reports of species showing altered behaviour, neurotoxicity assessment for species in the environment is not required and therefore mostly not performed. Considering the increasing numbers of environmental contaminants with potential neurotoxic potential, eco-neurotoxicity should be also considered in risk assessment. In order to do so novel test systems are needed that can cope with species differences within ecosystems. In the field, online-biomonitoring systems using behavioural information could be used to detect neurotoxic effects and effect-directed analyses could be applied to identify the neurotoxicants causing the effect. Additionally, toxic pressure calculations in combination with mixture modelling could use environmental chemical monitoring data to predict adverse effects and prioritize pollutants for laboratory testing. Cheminformatics based on computational toxicological data from in vitro and in vivo studies could help to identify potential neurotoxicants. An array of in vitro assays covering different modes of action could be applied to screen compounds for neurotoxicity. The selection of in vitro assays could be guided by AOPs relevant for eco-neurotoxicity. In order to be able to perform risk assessment for eco-neurotoxicity, methods need to focus on the most sensitive species in an ecosystem. A test battery using species from different trophic levels might be the best approach. To implement eco-neurotoxicity assessment into European risk assessment, cheminformatics and in vitro scre

Published
2018

15. Ingestion and contact with polyethylene microplastics does not cause acute toxicity on marine zooplankton

Author

Beiras, Ricardo, Bellas, Juan, Cachot, Jérome, Cormier, B., Cousin, X., Engwall, M., Gambardella, C., Garaventa, F., Keiter, S., Le Bihanic, F., López-Ibáñez, S., Piazza, V., Rial, Diego, Tato, Tania, Vidal-Liñán, Leticia, Beiras, Ricardo, Bellas, Juan, Cachot, Jérome, Cormier, B., Cousin, X., Engwall, M., Gambardella, C., Garaventa, F., Keiter, S., Le Bihanic, F., López-Ibáñez, S., Piazza, V., Rial, Diego, Tato, Tania, and Vidal-Liñán, Leticia

Published
2018

16. An ecotoxicological view on neurotoxicity assessment

Author

Legradi, J. B., Di Paolo, C., Kraak, M. H. S., van der Geest, H. G., Schymanski, E. L., Williams, A. J., Dingemans, M. M. L., Massei, R., Brack, W., Cousin, X., Begout, M. -L, van der Oost, R., Carion, A., Suarez-Ulloa, V., Silvestre, F., Escher, B. I., Engwall, Magnus, Nilén, Greta, Keiter, Steffen, Pollet, D., Waldmann, P., Kienle, C., Werner, I., Haigis, A. -C, Knapen, D., Vergauwen, L., Spehr, M., Schulz, W., Busch, W., Leuthold, D., Scholz, S., vom Berg, C. M., Basu, N., Murphy, C. A., Lampert, A., Kuckelkorn, J., Grummt, T., Hollert, H., Legradi, J. B., Di Paolo, C., Kraak, M. H. S., van der Geest, H. G., Schymanski, E. L., Williams, A. J., Dingemans, M. M. L., Massei, R., Brack, W., Cousin, X., Begout, M. -L, van der Oost, R., Carion, A., Suarez-Ulloa, V., Silvestre, F., Escher, B. I., Engwall, Magnus, Nilén, Greta, Keiter, Steffen, Pollet, D., Waldmann, P., Kienle, C., Werner, I., Haigis, A. -C, Knapen, D., Vergauwen, L., Spehr, M., Schulz, W., Busch, W., Leuthold, D., Scholz, S., vom Berg, C. M., Basu, N., Murphy, C. A., Lampert, A., Kuckelkorn, J., Grummt, T., and Hollert, H.

Abstract

The numbers of potential neurotoxicants in the environment are raising and pose a great risk for humans and the environment. Currently neurotoxicity assessment is mostly performed to predict and prevent harm to human populations. Despite all the efforts invested in the last years in developing novel in vitro or in silico test systems, in vivo tests with rodents are still the only accepted test for neurotoxicity risk assessment in Europe. Despite an increasing number of reports of species showing altered behaviour, neurotoxicity assessment for species in the environment is not required and therefore mostly not performed. Considering the increasing numbers of environmental contaminants with potential neurotoxic potential, eco-neurotoxicity should be also considered in risk assessment. In order to do so novel test systems are needed that can cope with species differences within ecosystems. In the field, online-biomonitoring systems using behavioural information could be used to detect neurotoxic effects and effect-directed analyses could be applied to identify the neurotoxicants causing the effect. Additionally, toxic pressure calculations in combination with mixture modelling could use environmental chemical monitoring data to predict adverse effects and prioritize pollutants for laboratory testing. Cheminformatics based on computational toxicological data from in vitro and in vivo studies could help to identify potential neurotoxicants. An array of in vitro assays covering different modes of action could be applied to screen compounds for neurotoxicity. The selection of in vitro assays could be guided by AOPs relevant for eco-neurotoxicity. In order to be able to perform risk assessment for eco-neurotoxicity, methods need to focus on the most sensitive species in an ecosystem. A test battery using species from different trophic levels might be the best approach. To implement eco-neurotoxicity assessment into European risk assessment, cheminformatics and in vitro scre, Funding Agencies:Federal Ministry of Education and Research (BMBF) Norman Network Dutch Water companies BTO2018-2023 SOLUTIONS project (European Union) 603437

Published
2018
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17. Ingestion and contact with polyethylene microplastics does not cause acute toxicity on marine zooplankton

Author

Beiras, R., Bellas, J., Cachot, J., Cormier, Bettie, Cousin, X., Engwall, Magnus, Gambardella, C., Garaventa, F., Keiter, Steffen, Le Bihanic, F., López-Ibáñez, S., Piazza, V., Rial, D., Tato, T., Vidal-Liñán, L., Beiras, R., Bellas, J., Cachot, J., Cormier, Bettie, Cousin, X., Engwall, Magnus, Gambardella, C., Garaventa, F., Keiter, Steffen, Le Bihanic, F., López-Ibáñez, S., Piazza, V., Rial, D., Tato, T., and Vidal-Liñán, L.

Abstract

Toxicity of polyethylene microplastics (PE-MP) of size ranges similar to their natural food to zooplanktonic organisms representative of the main taxa present in marine plankton, including rotifers, copepods, bivalves, echinoderms and fish, was evaluated. Early life stages (ELS) were prioritized as testing models in order to maximize sensitivity. Treatments included particles spiked with benzophenone-3 (BP-3), a hydrophobic organic chemical used in cosmetics with direct input in coastal areas. Despite documented ingestion of both virgin and BP-3 spiked microplastics no acute toxicity was found at loads orders of magnitude above environmentally relevant concentrations on any of the invertebrate models. In fish tests some effects, including premature or reduced hatching, were observed after 12 d exposure at 10 mg L-1 of BP-3 spiked PE-MP. The results obtained do not support environmentally relevant risk of microplastics on marine zooplankton. Similar approaches testing more hydrophobic chemicals with higher acute toxicity are needed before these conclusions could be extended to other organic pollutants common in marine ecosystems. Therefore, the replacement of these polymers in consumer products must be carefully considered., Funding Agencies:Spanish Government (MINECO) PCIN-2015-187-C03-03 CTM2016-77945-C3 French National Research Agency (Agence National de La Recherche) ANR-15-JOCE-0002-05 Galician Government ED431C 2017/46

Published
2018
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18. An ecotoxicological view on neurotoxicity assessment

Author

Legradi, J. B., primary, Di Paolo, C., additional, Kraak, M. H. S., additional, van der Geest, H. G., additional, Schymanski, E. L., additional, Williams, A. J., additional, Dingemans, M. M. L., additional, Massei, R., additional, Brack, W., additional, Cousin, X., additional, Begout, M.-L., additional, van der Oost, R., additional, Carion, A., additional, Suarez-Ulloa, V., additional, Silvestre, F., additional, Escher, B. I., additional, Engwall, M., additional, Nilén, G., additional, Keiter, S. H., additional, Pollet, D., additional, Waldmann, P., additional, Kienle, C., additional, Werner, I., additional, Haigis, A.-C., additional, Knapen, D., additional, Vergauwen, L., additional, Spehr, M., additional, Schulz, W., additional, Busch, W., additional, Leuthold, D., additional, Scholz, S., additional, vom Berg, C. M., additional, Basu, N., additional, Murphy, C. A., additional, Lampert, A., additional, Kuckelkorn, J., additional, Grummt, T., additional, and Hollert, H., additional

Published
2018
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19. Ingestion and contact with polyethylene microplastics does not cause acute toxicity on marine zooplankton

Author

Beiras, R., primary, Bellas, J., additional, Cachot, J., additional, Cormier, B., additional, Cousin, X., additional, Engwall, M., additional, Gambardella, C., additional, Garaventa, F., additional, Keiter, S., additional, Le Bihanic, F., additional, López-Ibáñez, S., additional, Piazza, V., additional, Rial, D., additional, Tato, T., additional, and Vidal-Liñán, L., additional

Published
2018
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22. ESTHER, the database of the alpha/beta-hydrolase fold superfamily of proteins

Author

Hotelier, T., Renault, L.L.R., Cousin, X., Negre, V., Marchot, P., Chatonnet, A., Services déconcentrés d'appui à la recherche - Montpellier, Institut National de la Recherche Agronomique (INRA), Biochimie - Ingénierie des protéines, Université de la Méditerranée - Aix-Marseille 2-Centre National de la Recherche Scientifique (CNRS), Différenciation Cellulaire et Croissance (DCC), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2), and Centre National de la Recherche Scientifique (CNRS)-Université de la Méditerranée - Aix-Marseille 2

Subjects
base de donnée, esther, [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs]
Abstract

The alpha/beta-hydrolase fold is characterized by a beta-sheet core of five to eight strands connected by alpha-helices to form a alpha/beta/alpha sandwich. In most of the family members the beta-strands are parallels, but some show an inversion in the order of the first strands, resulting in antiparallel orientation. The members of the superfamily diverged from a common ancestor into a number of hydrolytic enzymes with a wide range of substrate specificities, together with other proteins with no recognized catalytic activity. In the enzymes the catalytic triad residues are presented on loops, of which one, the nucleophile elbow, is the most conserved feature of the fold. Of the other proteins, which all lack from one to all of the catalytic residues, some may simply be 'inactive' enzymes while others are known to be involved in surface recognition functions. The ESTHER database (http://bioweb.ensam.inra.fr/esther) gathers and annotates all the published information related to gene and protein sequences of this superfamily, as well as biochemical, pharmacological and structural data, and connects them so as to provide the bases for studying structure-function relationships within the family. The most recent developments of the database, which include a section on human diseases related to members of the family, are described.

Published
2004
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25. Acetylcholinesterase from Bungarus venom: a monomeric species.

Author

UCL, Cousin, X, Créminon, C, Grassi, J., Méflah, K, Cornu, Guy, Saliou, B, Bon, S, Massoulié, J, Bon, C, UCL, Cousin, X, Créminon, C, Grassi, J., Méflah, K, Cornu, Guy, Saliou, B, Bon, S, Massoulié, J, and Bon, C

Abstract

The venom of Bungarus fasciatus, an Elapidae snake, contains a high level of AChE activity. Partial peptide sequences show that it is closely homologous to other AChEs. Bungarus venom AChE is a non-amphiphilic monomeric species, a molecular form of AChE which has not been previously found in significant levels in other tissues. The composition of carbohydrates suggests the presence of N-glycans of the 'complex' and 'hybrid' types. Ion exchange chromatography, isoelectric focusing and electrophoresis in non-denaturing and denaturing conditions reveal a complex microheterogeneity of this enzyme, which is partly related to its glycosylation.

Published
1996

26. Allometric relationship between body mass and aerobic metabolism in zebrafish Danio rerio.

Author

Lucas, J., Schouman, A., Lyphout, L., Cousin, X., and Lefrancois, C.

Subjects
ALLOMETRIC equations, BODY mass index, AEROBIC metabolism, ZEBRA danio, FISH metabolism
Abstract

The relationship between body mass ( M) and metabolic rate was investigated through the assessment of active ( RA) and standard ( RS) metabolic rate at different life stages in zebrafish Danio rerio (5 day-old larvae, 2 month-old juveniles and 6 month-old adults). Scaling exponents and constants were assessed for standard ( RS = 0·273 M0·965 in mgO2 g−1 h−1) and active metabolic rate ( RA = 0·799 M0·926 in mgO2 g−1 h−1). These data provide the basis for further experiments regarding the effects of environmental factors on aerobic metabolism throughout the life cycle of this species. [ABSTRACT FROM AUTHOR]

Published
2014
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40. Identification of a novel type of alternatively spliced exon from the acetylcholinesterase gene of Bungarus fasciatus. Molecular forms of acetylcholinesterase in the snake liver and muscle.

Author

Cousin, X, Bon, S, Massoulié, J, and Bon, C

Abstract

The venom of the snake Bungarus fasciatus contains a hydrophilic, monomeric species of acetylcholinesterase (AChE), characterized by a C-terminal region that does not resemble the alternative T- or H-peptides. Here, we show that the snake contains a single gene for AChE, possessing a novel alternative exon (S) that encodes the C-terminal region of the venom enzyme, located downstream of the T exon. Alternative splicing generates S mRNA in the venom gland and S and T mRNAs in muscle and liver. We found no evidence for the presence of an H exon between the last common "catalytic" exon and the T exon, where H exons are located in Torpedo and in mammals. Moreover, COS cells that were transfected with AChE expression vectors containing the T exon with or without the preceding genomic region produced exclusively AChET subunits. In the snake tissues, we could not detect any glycophosphatidylinositol-anchored AChE form that would have derived from H subunits. In the liver, the cholinesterase activity comprises both AChE and butyrylcholinesterase components; butyrylcholinesterase corresponds essentially to nonamphiphilic tetramers and AChE to nonamphiphilic monomers (G1na). In muscle, AChE is largely predominant: it consists of globular forms (G1a and G4a) and trace amounts of asymmetric forms (A8 and A12), which derive from AChET subunits. Thus, the Bungarus AChE gene possesses alternatively spliced T and S exons but no H exon; the absence of an H exon may be a common feature of AChE genes in reptiles and birds.

Published
1998

41. Cloning and expression of acetylcholinesterase from Bungarus fasciatus venom. A new type of cooh-terminal domain; involvement of a positively charged residue in the peripheral site.

Author

Cousin, X, Bon, S, Duval, N, Massoulié, J, and Bon, C

Abstract

As deduced from cDNA clones, the catalytic domain of Bungarus fasciatus venom acetylcholinesterase (AChE) is highly homologous to those of other AChEs. It is, however, associated with a short hydrophilic carboxyl-terminal region, containing no cysteine, that bears no resemblance to the alternative COOH-terminal peptides of the GPI-anchored molecules (H) or of other homomeric or heteromeric tailed molecules (T). Expression of complete and truncated AChE in COS cells showed that active hydrophilic monomers are produced and secreted in all cases, and that cleavage of a very basic 8-residue carboxyl-terminal fragment occurs upon secretion. The COS cells produced Bungarus AChE about 30 times more efficiently than an equivalent secreted monomeric rat AChE. The recombinant Bungarus AChE, like the natural venom enzyme, showed a distinctive ladder pattern in nondenaturing electrophoresis, probably reflecting a variation in the number of sialic acids. By mutagenesis, we showed that two differences (methionine instead of tyrosine at position 70; lysine instead of aspartate or glutamate at position 285) explain the low sensitivity of Bungarus AChE to peripheral site inhibitors, compared to the Torpedo or mammalian AChEs. These results illustrate the importance of both the aromatic and the charged residues, and the fact that peripheral site ligands (propidium, gallamine, D-tubocurarine, and fasciculin 2) interact with diverse subsets of residues.

Published
1996

43. DEVELOPMENT OF GENOMIC TOOLS IN SENEGALESE SOLE: TRANSCRIPTOME ASSEMBLY, ANNOTATED DATABASE, MICROARRAY AND GENOME DRAFT

Author

Manchado, M., Benzekri, H., Seoane, P., Bautista, R., Sanchez, J. J., Cousin, X., Planas, J. V., Rebordinos, L., Manuel Gonzalo Claros, Instituto Andaluz de Investigación y Formación Agraria y Pesquera (IFAPA), Universidad de Málaga [Málaga] = University of Málaga [Málaga], Plataforma Andaluza de Bioinformática, Instituto Nacional de Toxicología y Ciencias Forenses, Partenaires INRAE, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Universitat Autònoma de Barcelona (UAB), University of Barcelona, Laboratorio de Genética, and Universidad Complutense de Madrid = Complutense University of Madrid [Madrid] (UCM)

Subjects
base de données, solea senegalensis, [SDV]Life Sciences [q-bio], transcriptome, genome, draft, database, microarray, poisson, [INFO]Computer Science [cs], sole, analyse génomique, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

44. An ecotoxicological view on neurotoxicity assessment

Author

Legradi, Jessica, Di Paolo, Carolina, Kraak, M. H. S., Van Der Geest, H. G., Schymanski, E. L., Williams, A. J., Dingemans, M. M. L., Massei, R., Brack, W., Cousin, X., Begout, M.-L., Van Der Oost, Ron, Carion, A., Suarez-Ulloa, V., Silvestre, F., Escher, B. I., Engwall, M., Nilén, G., Keiter, S. H., Pollet, D., Waldmann, P., Kienle, C., Werner, I., Haigis, Ann-Cathrin, Knapen, D., Vergauwen, L., Spehr, Marc, Schulz, W., Busch, W., Leuthold, D., Scholz, S., Vom Berg, C. M., Basu, N., Murphy, C. A., Lampert, Angelika, Kuckelkorn, J., Grummt, T., and Hollert, Henner

Subjects
13. Climate action, 16. Peace & justice
Abstract

Environmental sciences Europe : ESEU 30(1), 46 (2018). doi:10.1186/s12302-018-0173-x special issue: "Special Issue 20 Years SETAC GLB / Edited by: Jochen Zubrod, Bettina Hitzfeld, Marion Junghans, Anja Kehrer, Rolf Düring, Peter Ebke, Dominic Kaiser, Anja Kehrer, Silvio Knaebe, Nadine Ruchter, Tobias Frische and Henner Hollert", Published by Springer, Heidelberg

45. Genome-wide gene expression analysis during Solea sp. embryo-larval development

Author

Cousin, X., Claros, M. G., Mazurais, D., Bautista, R., Benzekri, H., Begout, M-L, Ponce, M., Armesto, P., Zambonino, J., Planas, J. V., and Manuel Manchado

Subjects
Animal biology, solea senegalensis, Genome, génome, Embryonic Development, High-Throughput Nucleotide Sequencing, soleidae, solea solea, poisson, développement embryonnaire, Larva, Biologie animale, Flatfishes, Animals, sole, développement larvaire, Transcriptome, expression des gènes

47. Long-term impact of embryonic exposure to ethinylestradiol and clotrimazole on behavior and neuroplasticity in zebrafish (Danio rerio).

Author

Blanc-Legendre M, Guillot L, Chevalier L, Malleret C, Le Menach K, Pardon P, Budzinski H, Brion F, Sire S, Coumailleau P, Charlier TD, Pellegrini E, and Cousin X

Abstract

Estrogen receptors (ER) are widely expressed in the brain of many species and experimental results highlighted the role of estradiol in neuronal plasticity and behavior. Consequently, the brain is therefore a prime target for endocrine disrupting chemicals (EDCs) interacting with estrogen signaling. Very little is known about the late effects of early disruption of estrogen signaling by EDCs. We focused on: ethinylestradiol (EE2; ER agonist) and clotrimazole (inhibitor of key steroidogenesis enzymes, including aromatases). Zebrafish eleutheroembryos were exposed (0-5 days) and then raised normally until adulthood. Several behavioral tests were performed in adults, then cell proliferation and dopaminergic neurons were quantified in several brain regions using immunostaining. Overall, a developmental exposure to EDCs stimulates cell proliferation in the dorsal telencephalon. At environmentally-relevant concentrations, male fish exposed to EE2 exhibited increased activity levels and decreased social behavior, posing a potential risk to population balance and health., Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Cousin Xavier reports financial support was provided by French National Institute for Agricultural Research INRAE. Charlier Thierry reports financial support was provided by Research Institute for Environmental and Occupational Health. Budzinski Helene reports financial support was provided by University of Bordeaux. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

Published
2024
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48. The time for ambitious action is now: Science-based recommendations for plastic chemicals to inform an effective global plastic treaty.

Author

Brander SM, Senathirajah K, Fernandez MO, Weis JS, Kumar E, Jahnke A, Hartmann NB, Alava JJ, Farrelly T, Almroth BC, Groh KJ, Syberg K, Buerkert JS, Abeynayaka A, Booth AM, Cousin X, Herzke D, Monclús L, Morales-Caselles C, Bonisoli-Alquati A, Al-Jaibachi R, and Wagner M

Subjects
United Nations, Environmental Policy, Environmental Pollutants analysis, Humans, International Cooperation, Plastics, Environmental Pollution prevention & control
Abstract

The ubiquitous and global ecological footprint arising from the rapidly increasing rates of plastic production, use, and release into the environment is an important modern environmental issue. Of increasing concern are the risks associated with at least 16,000 chemicals present in plastics, some of which are known to be toxic, and which may leach out both during use and once exposed to environmental conditions, leading to environmental and human exposure. In response, the United Nations member states agreed to establish an international legally binding instrument on plastic pollution, the global plastics treaty. The resolution acknowledges that the treaty should prevent plastic pollution and its related impacts, that effective prevention requires consideration of the transboundary nature of plastic production, use and pollution, and that the full life cycle of plastics must be addressed. As a group of scientific experts and members of the Scientists' Coalition for an Effective Plastics Treaty, we concur that there are six essential "pillars" necessary to truly reduce plastic pollution and allow for chemical detoxification across the full life cycle of plastics. These include a plastic chemical reduction and simplification, safe and sustainable design of plastic chemicals, incentives for change, holistic approaches for alternatives, just transition and equitable interventions, and centering human rights. There is a critical need for scientifically informed and globally harmonized information, transparency, and traceability criteria to protect the environment and public health. The right to a clean, healthy, and sustainable environment must be upheld, and thus it is crucial that scientists, industry, and policy makers work in concert to create a future free from hazardous plastic contamination., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Susanne M Brander reports financial support was provided by National Science Foundation. Andy Booth reports financial support was provided by The Research Council of Norway. Andy Booth reports financial support was provided by European Commission. Annika Jahnke reports financial support was provided by Heimholtz Association. Dorte Hertz reports financial support was provided by The Research Council of Norway. Amila Abeynayaka reports financial support was provided by European Union Horizon Europe Research and Innovation. Susanne Brander reports a relationship with Southern California Coastal Water Research Project that includes: consulting or advisory. Brander previously consulted for the Environmental Defense fund and is on advisory boards or groups for the Plastic Pollution Coalition, as well as the Department of Toxic Substances Control (California) and the San Francisco Estuary Institute. Martin Wagner is an unremunerated member of the Scientific Advisory Board of the Food Packaging Forum Foundation and received travel support for attending annual board meetings. Marina Fernandez is a member of the Endocrine Society's delegation at the Intergovernmental Negotiating Committee (INC) to develop an international legally binding instrument on plastic pollution. All authors are members of the Scientists Coalition for an Effective Plastics Treaty, and Susanne Brander, Martin Wagner, Marina Fernandez, Trisia Farrelly, Kristian Syberg, Amila Abeynayaka, and Bethanie Carney Alroth are Scientists Coalition Steering Committee Members. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Crown Copyright © 2024. Published by Elsevier B.V. All rights reserved.)

Published
2024
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49. Early rearing of European seabass (Dicentrarchus labrax) with mild current enrichment modifies fish swimming behavior without altering their growth performance.

Author

Magnoni LJ, Cousin X, Leguen I, Espirito-Santo C, Clota F, Lallement S, and Bégout ML

Subjects
Animals, Hematocrit, Behavior, Animal physiology, Swimming physiology, Bass physiology, Hydrocortisone blood
Abstract

The implementation of conditions that favor optimum swimming activity (e.g., suitable flow regimes), has been associated with enhanced growth and improved welfare in some farmed fish species. Despite the importance of European seabass in aquaculture, the potential beneficial effects of rearing flow conditions have not been sufficiently explored in this species. This study investigates how the application of fast (F, 0.01-0.20 m s -1 ) or slow (S, <0.01 m s -1 ) steady flows in rearing tanks for 75-77 days affected physiological and behavioral traits in seabass fingerlings. Growth performance, external and internal morphology, and several physiological variables, including hematocrit, plasma cortisol concentration, and osmotic and ionic balance were not affected by flow conditions during rearing. Also, behavioral tests implemented in groups or isolated individuals suggest that coping styles were not affected by the two tank-rearing conditions. On the contrary, the swimming behavior assessed in tests was modified by the flow condition experienced during rearing. Mean swimming speed, peak acceleration, swimming distance, angular velocity, and meander showed some variability across different tests and time, although consistently displaying higher values in seabass reared in the F condition, suggesting increased activity and more consistent swimming patterns in that group. However, the cumulative time in proximity between individuals measured in behavioral group tests suggested that group cohesion was variable, without displaying differences between F and S groups. These findings have particularly important implications for fish welfare and may suggest plasticity in the behavioral response to rearing conditions for this species, although not affecting the assignment of the individuals to the different coping styles. SUMMARY STATEMENT: We investigated how mild current enrichment applied during early rearing in seabass modifies physiological and behavioral responses involving swimming activity, exploring the potential associations to fish welfare., Competing Interests: Declaration of competing interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as potential conflicts of interest., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Published
2025
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50. Development of a Zebrafish Embryo-Based Test System for Thyroid Hormone System Disruption: 3Rs in Ecotoxicological Research.

Author

Gölz L, Blanc-Legendre M, Rinderknecht M, Behnstedt L, Coordes S, Reger L, Sire S, Cousin X, Braunbeck T, and Baumann L

Abstract

There is increasing concern regarding pollutants disrupting the vertebrate thyroid hormone (TH) system, which is crucial for development. Thus, identification of TH system-disrupting chemicals (THSDCs) is an important requirement in the Organisation for Economic Co-operation and Development (OECD) testing framework. The current OECD approach uses different model organisms for different endocrine modalities, leading to a high number of animal tests. Alternative models compatible with the 3Rs (replacement, reduction, refinement) principle are required. Zebrafish embryos, not protected by current European Union animal welfare legislation, represent a promising model. Studies show that zebrafish swim bladder inflation and eye development are affected by THSDCs, and the respective adverse outcome pathways (AOPs) have been established. The present study compared effects of four THSDCs with distinct molecular modes of action: Propylthiouracil (PTU), potassium perchlorate, iopanoic acid, and the TH triiodothyronine (T3) were tested with a protocol based on the OECD fish embryo toxicity test (FET). Effects were analyzed according to the AOP concept from molecular over morphological to behavioral levels: Analysis of thyroid- and eye-related gene expression revealed significant effects after PTU and T3 exposure. All substances caused changes in thyroid follicle morphology of a transgenic zebrafish line expressing fluorescence in thyrocytes. Impaired eye development and swimming activity were observed in all treatments, supporting the hypothesis that THSDCs cause adverse population-relevant changes. Findings thus confirm that the FET can be amended by TH system-related endpoints into an integrated protocol comprising molecular, morphological, and behavioral endpoints for environmental risk assessment of potential endocrine disruptors, which is compatible with the 3Rs principle. Environ Toxicol Chem 2024;00:1-18. © 2024 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC., (© 2024 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.)

Published
2024
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