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213 results on '"Courtney, Michael J."'

2. The First Modified Delphi Consensus-Building Exercise on Surgical Ward Rounds in the United Kingdom National Health Service

Author

Amr, Bassem, Omar, Islam, Abdelrahman, Mohamed, Abdalkoddus, Muhammad, Abou El Ella, Yasmin, Abouelazayem, Mohamed, Abubakr, Mohammed O. A., Andrews, Sian, Awad, Sherif, Bunting, David, Callejas-Diaz, Lindes, Cambridge, William A., Carr, William, Chesworth, Angela, Courtney, Michael J., edge, Jemma, Froghi, Farid, Gilliam, Andrew, graham, Yitka, Harwood, Sarah, Hollyman, Marianne, Kimble, Adam, kotb, Ahmed, Majithia, Ridhi, Mallinson, Sue, Metcalfe, Nicky, O’Kane, Mary, Parmar, Chetan, Saiyed, Saniya, Shaban, Mohamed, Singhal, Rishi, Trewin, Lisa, Wadhawan, Himanshu, and Mahawar, Kamal

Published
2023
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6. Atomistic simulations reveal impacts of missense mutations on the structure and function of SynGAP1.

Author

Ali, Aliaa E, Li, Li-Li, Courtney, Michael J, Pentikäinen, Olli T, and Postila, Pekka A

Subjects
MISSENSE mutation, DRUG discovery, ALZHEIMER'S disease, MOLECULAR dynamics, MUTAGENESIS
Abstract

De novo mutations in the synaptic GTPase activating protein (SynGAP) are associated with neurological disorders like intellectual disability, epilepsy, and autism. SynGAP is also implicated in Alzheimer's disease and cancer. Although pathogenic variants are highly penetrant in neurodevelopmental conditions, a substantial number of them are caused by missense mutations that are difficult to diagnose. Hence, in silico mutagenesis was performed for probing the missense effects within the N-terminal region of SynGAP structure. Through extensive molecular dynamics simulations, encompassing three 150-ns replicates for 211 variants, the impact of missense mutations on the protein fold was assessed. The effect of the mutations on the folding stability was also quantitatively assessed using free energy calculations. The mutations were categorized as potentially pathogenic or benign based on their structural impacts. Finally, the study introduces wild-type-SynGAP in complex with RasGTPase at the inner membrane, while considering the potential effects of mutations on these key interactions. This study provides structural perspective to the clinical assessment of SynGAP missense variants and lays the foundation for future structure-based drug discovery. [ABSTRACT FROM AUTHOR]

Published
2024
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14. Preoperative risk factors for conversion from laparoscopic to open cholecystectomy: a validated risk score derived from a prospective U.K. database of 8820 patients

Author

Fenwick, Stephen, Elmasry, Mohamed, Nunes, Quentin, Kennedy, David, Khan, Raja B., Khan, Muhammad A.S., Magee, Conor J., Jones, Steven M., Mason, Denise, Parappally, Ciny P., Mathur, Pawan, Saunders, Michael, Jamel, Sara, Haque, Samer U.l., Zafar, Sara, Shiwani, Muhammad H., Samuel, Nehemiah, Dar, Farooq, Jackson, Andrew, Lovett, Bryony, Dindyal, Shiva, Winter, Hannah, Rahman, Saquib, Wheatley, Kevin, Nieto, Tom, Ayaani, Soofiyah, Youssef, Haney, Nijjar, Rajwinder S., Watkin, Helen, Naumann, David, Emeshi, Sophie, Sarmah, Piyush B., Lee, Kathryn, Joji, Nikita, Heath, Jonathan, Teasdale, Rebecca L., Weerasinghe, Chamindri, Needham, Paul J., Welbourn, Hannah, Forster, Luke, Finch, David, Blazeby, Jane M., Robb, William, McNair, Angus G.K., Hrycaiczuk, Alex, Charalabopoulos, Alexandros, Kadirkamanathan, Sritharan, Tang, Cheuk-Bong, Jayanthi, Naga V.G., Noor, Nigel, Dobbins, Brian, Cockbain, Andrew J., Nilsen-Nunn, April, de Siqueira, Jonathan, Pellen, Mike, Cowley, Jonathan B., Ho, Wei-Min, Miu, Victor, White, Timothy J., Hodgkins, Kathryn A., Kinghorn, Alison, Tutton, Matthew G., Al-Abed, Yahya A., Menzies, Donald, Ahmad, Anwar, Reed, Joanna, Khan, Shabuddin, Monk, David, Vitone, Louis J., Murtaza, Ghulam, Joel, Abraham, Brennan, Stephen, Shier, David, Zhang, Catherine, Yoganathan, Thusidaran, Robinson, Steven J., McCallum, Iain J.D., Jones, Michael J., Elsayed, Mohammed, Tuck, Liz, Wayman, John, Carney, Kate, Aroori, Somaiah, Hosie, Kenneth B., Kimble, Adam, Bunting, David M., Fawole, Adeshina S., Basheer, Mohammed, Dave, Rajiv V., Sarveswaran, Janahan, Jones, Elinor, Kendal, Chris, Tilston, Michael P., Gough, Martin, Wallace, Tom, Singh, Shailendra, Downing, Justine, Mockford, Katherine A., Issa, Eyad, Shah, Nayab, Chauhan, Neal, Wilson, Timothy R., Forouzanfar, Amir, Wild, Jonathan R.L., Nofal, Emma, Bunnell, Catherine, Madbak, Khaliel, Rao, Sudhindra T.V., Devoto, Laurence, Siddiqi, Najaf, Khawaja, Zechan, Hewes, James C., Gould, Laura, Chambers, Alice, Rodriguez, Daniel U., Sen, Gourab, Robinson, Stuart, Bartlett, Francis, Rae, David M., Stevenson, Thomas E.J., Sarvananthan, Kas, Dwerryhouse, Simon J., Higgs, Simon M., Old, Oliver J., Hardy, Thomas J., Shah, Reena, Hornby, Steve T., Keogh, Ken, Frank, Lucinda, Al-Akash, Musallam, Upchurch, Emma A., Frame, Richard J., Hughes, Michael, Jelley, Clare, Weaver, Simon, Roy, Sudipta, Sillo, Toritseju O., Galanopoulos, Giorgios, Cuming, Tamzin, Cunha, Pedro, Tayeh, Salim, Kaptanis, Sarantos, Heshaishi, Mohamed, Eisawi, Abdalla, Abayomi, Michael, Ngu, Wee S., Fleming, Katie, Bajwa, Dalvir S., Chitre, Vivek, Aryal, Kamal, Ferris, Paul, Silva, Michael, Lammy, Simon, Mohamed, Sarah, Khawaja, Amir, Hussain, Adnan, Ghazanfar, Mudassar A., Bellini, Maria I., Ebdewi, Hamdi, Elshaer, Mohamed, Gravante, Gianpiero, Drake, Benjamin, Ogedegbe, Arikoge, Mukherjee, Dipankar, Arhi, Chanpreet, Giwa, Lola, Iqbal, Nusrat, Watson, Nicholas F., Aggarwal, Smeer K., Orchard, Philippa, Villatoro, Eduardo, Willson, Peter D., Mok, Kam W.J., Woodman, Thomas, Deguara, Jean, Garcea, Giuseppe, Babu, Benoy I., Dennison, Alistair R., Malde, Deep, Lloyd, David, Slavin, John P., Jones, Robert P., Ballance, Laura, Gerakopoulos, Stratos, Jambulingam, Periyathambi, Mansour, Sami, Sakai, Naomi, Acharya, Vikas, Sadat, Mohammed M., Karim, Lawen, Larkin, David, Amin, Khalid, Khan, Amarah, Law, Jennifer, Jamdar, Saurabh, Smith, Stella R., Sampat, Keerthika, O'shea, Kathryn M., Manu, Mangta, Asprou, Fotini M., Malik, Nabeela S., Chang, Jessica, Johnstone, Marianne, Lewis, Michael, Roberts, Geoffrey P., Karavadra, Babu, Photi, Evangelos, Hewes, James, Rodriguez, Dan, O'Reilly, Derek A., Rate, Anthony J., Sekhar, Hema, Henderson, Lucy T., Starmer, Benjamin Z., Coe, Peter O., Tolofari, Sotonye, Barrie, Jenifer, Bashir, Gareth, Sloane, Jake, Madanipour, Suroosh, Halkias, Constantine, Trevatt, Alexander E.J., Borowski, David W., Hornsby, Jane, Courtney, Michael J., Virupaksha, Suvi, Seymour, Keith, Robinson, Sarah, Hawkins, Helen, Bawa, Sadiq, Gallagher, Paul V., Reid, Alistair, Wood, Peter, Finch, Jonathan G., Finch, J.Guy, Parmar, Jitesh, Stirland, Euan, Gardner-Thorpe, James, Al-Muhktar, Ahmed, Peterson, Mark, Majeed, Ali, Bajwa, Farrukh M., Martin, Jack, Choy, Alfred, Tsang, Andrew, Pore, Naresh, Andrew, David R., Al-Khyatt, Waleed, Santosh Bhandari, Christopher Taylor, Chambers, Adam, Subramanium, Dhivya, Toh, Simon K.C., Carter, Nicholas C., Mercer, Stuart J., Knight, Benjamin, Vijay, Vardhini, Alagaratnam, Swethan, Sinha, Sidhartha, Khan, Shahab, El-Hasani, Shamsi S., Hussain, Abdulzahra A., Bhattacharya, Vish, Kansal, Nisheeth, Fasih, Tani, Jackson, Claire, Siddiqui, Midhat N., Chishti, Imran A., Fordham, Imogen J., Siddiqui, Zohaib, Bausbacher, Harald, Geogloma, Ileana, Gurung, Kabita, Tsavellas, George, Basynat, Pradeep, Shrestha, Ashish K., Basu, Sanjoy, Chhabra, Alok, Harilingam, Mohan, Rabie, Mohamed, Akhtar, Mansoor, Kumar, Pradeep, Jafferbhoy, Sadaf F., Hussain, Najam, Raza, Soulat, Haque, Manzarul, Alam, Imran, Aseem, Rabiya, Patel, Shakira, Asad, Mehek, Booth, Michael I., Ball, William R., Wood, Christopher P.J., Pinho-Gomes, Ana C., Kausar, Ambareen, Obeidallah, Mohammed, Varghase, Joseph, Lodhia, Joshil, Bradley, Donal, Rengifo, Carla, Lindsay, David, Gopalswamy, Sivakumar, Finlay, Ian, Wardle, Stacy, Bullen, Naomi, Iftikhar, Syed Y., Awan, Altaf, Ahmed, Javed, Leeder, Paul, Fusai, Guiseppe, Bond-Smith, Giles, Psica, Alicja, Puri, Yogesh, Hou, David, Noble, Fergus, Szentpali, Karoly, Broadhurst, Jack, Date, Ravindra, Hossack, Martin R., Goh, Yan L., Turner, Paul, Shetty, Vinutha, Riera, Manel, Macano, Christina A.W., Sukha, Anisha, Preston, Shaun R., Hoban, Jennifer R., Puntis, Daniel J., Williams, Sophie V., Krysztopik, Richard, Kynaston, James, Batt, Jeremy, Doe, Matthew, Goscimski, Andrzej, Jones, Gareth H., Hall, Claire, Carty, Nick, Ahmed, Jamil, Panteleimonitis, Sofoklis, Gunasekera, Rohan T., Sheel, Andrea R.G., Lennon, Hannah, Hindley, Caroline, Reddy, Marcus, Kenny, Ross, Elkheir, Natalie, McGlone, Emma R., Rajaganeshan, Rajasundaram, Hancorn, Kate, Hargreaves, Anita, Prasad, Raj, Longbotham, David A., Vijayanand, Dhakshinamoorthy, Wijetunga, Imeshi, Ziprin, Paul, Nicolay, Christopher R., Yeldham, Geoffrey, Read, Edward, Gossage, James A., Rolph, Rachel C., Ebied, Husam, Phull, Manraj, Khan, Mohammad A., Popplewell, Matthew, Kyriakidis, Dimitrios, Hussain, Anwar, Henley, Natasha, Packer, Jessica R., Derbyshire, Laura, Porter, Jonathan, Appleton, Shaun, Farouk, Marwan, Basra, Melvinder, Jennings, Neil A., Ali, Shahda, Kanakala, Venkatesh, Ali, Haythem, Lane, Risha, Dickson-Lowe, Richard, Zarsadias, Prizzi, Mirza, Darius, Puig, Sonia, Al Amari, Khalid, Vijayan, Deepak, Sutcliffe, Robert, Marudanayagam, Ravi, Hamady, Zayed, Prasad, Abheesh R., Patel, Abhilasha, Durkin, Damien, Kaur, Parminder, Bowen, Laura, Byrne, James P., Pearson, Katherine L., Delisle, Theo G., Davies, James, Tomlinson, Mark A., Johnpulle, Michelle A., Slawinski, Corinna, Macdonald, Andrew, Nicholson, James, Newton, Katy, Mbuvi, James, Farooq, Ansar, Mothe, Bhavani S., Zafrani, Zakhi, Brett, Daniel, Francombe, James, Spreadborough, Philip, Barnes, James, Cheung, Melanie, Al-Bahrani, Ahmed Z., Preziosi, Giuseppe, Urbonas, Tomas, Alberts, Justin, Mallik, Mekhlola, Patel, Krashna, Segaran, Ashvina, Doulias, Triantafyllos, Sufi, Pratik A., Yao, Caroline, Pollock, Sarah, Manzelli, Antonio, Wajed, Saj, Kourkulos, Michail, Pezzuto, Roberto, Wadley, Martin, Hamilton, Emma, Jaunoo, Shameen, Padwick, Robert, Sayegh, Mazin, Newton, Richard C., Hebbar, Madhusoodhana, Farag, Sameh F., Hebbar, Madhu, Spearman, John, Hamdan, Mohammed F., D'Costa, Conrad, Blane, Christine, Giles, Mathew, Peter, Mark B., Hirst, Natalie A., Hossain, Tanvir, Pannu, Arslan, El-Dhuwaib, Yesar, Morrison, Tamsin E.M., Taylor, Greg W., Thompson, Ronald L.E., McCune, Ken, Loughlin, Paula, Lawther, Roger, Byrnes, Colman K., Simpson, Duncan J., Mawhinney, Abi, Warren, Conor, McKay, Damian, McIlmunn, Colin, Martin, Serena, MacArtney, Matthew, Diamond, Tom, Davey, Phil, Jones, Claire, Clements, Joshua M., Digney, Ruairi, Chan, Wei M., McCain, Stephen, Gull, Sadaf, Janeczko, Adam, Dorrian, Emmet, Harris, Andrew, Dawson, Suzanne, Johnston, Dorothy, McAree, Barry, Ghareeb, Essam, Thomas, George, Connelly, Martin, McKenzie, Stephen, Cieplucha, Krzysztos, Spence, Gary, Campbell, William, Hooks, Gareth, Bradley, Neil, Hill, Arnold D.K., Cassidy, John T., Boland, Michael, Burke, Paul, Nally, Deirdre M., Khogali, Elmoataz, Shabo, Wael, Iskandar, Edrin, McEntee, Gerry P., O'Neill, Maeve A., Peirce, Colin, Lyons, Emma M., O'Sullivan, Adrian W., Thakkar, Rohan, Carroll, Paul, Ivanovski, Ivan, Balfe, Paul, Lee, Matthew, Winter, Des C., Kelly, Michael E., Hoti, Emir, Maguire, Donal, Karunakaran, Priyadarssini, Geoghegan, Justin G., Martin, Sean T., Cross, Keith S., Cooke, Fiachra, Zeeshan, Saquib, Murphy, James O., Mealy, Ken, Mohan, Helen M., Nedujchelyn, Yuwaraja, Ullah, Muhammad F., Ahmed, Irfan, Giovinazzo, Francesco, Milburn, James, Prince, Sarah, Brooke, Eleanor, Buchan, Joanna, Khalil, Ahmed M., Vaughan, Elizabeth M., Ramage, Michael I., Aldridge, Roland C., Gibson, Simon, Nicholson, Gary A., Vass, David G., Grant, Alan J., Holroyd, David J., Jones, Angharad, Sutton, Cherith M.L.R., O'Dwyer, Patrick, Nilsson, Frida, Weber, Beatrix, Williamson, Tracey K., Lalla, Kushik, Bryant, Alice, Carter, Ross, Forrest, Craig R., Hunter, David I., Nassar, Ahmad H., Orizu, Mavis N., Knight, Katrina, Qandeel, Haitham, Suttie, Stuart, Belding, Rowena, McClarey, Andrew, Boyd, Alan T., Guthrie, Graeme J.K., Lim, Pei J., Luhmann, Andreas, Watson, Angus J.M., Richards, Colin H., Nicol, Laura, Madurska, Marta, Harrison, Ewen, Boyce, Kathryn M., Roebuck, Amanda, Ferguson, Graeme, Pati, Pradeep, Wilson, Michael S.J., Dalgaty, Faith, Fothergill, Laura, Driscoll, Peter J., Mozolowski, Kirsty L., Banwell, Victoria, Bennett, Stephen P., Rogers, Paul N., Skelly, Brendan L., Rutherford, Claire L., Mirza, Ahmed K., Lazim, Taha, Lim, Henry C.C., Duke, Diana, Ahmed, Talat, Beasley, William D., Wilkinson, Marc D., Maharaj, Geta, Malcolm, Cathy, Brown, Timothy H., Shingler, Guy M., Mowbray, Nicholas, Radwan, Rami, Morcous, Paul, Wood, Simon, Kadhim, Abbas, Stewart, Duncan J., Baker, Andrew L., Tanner, Nicola, Shenoy, Hrishikesh, Sutcliffe, Robert P., Hollyman, Marianne, Hodson, James, Bonney, Glenn, Vohra, Ravi S., and Griffiths, Ewen A.

Published
2016
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20. Utilisation of an operative difficulty grading scale for laparoscopic cholecystectomy

Author

Griffiths, Ewen A, Hodson, James, Vohra, Ravi S, Marriott, Paul, Katbeh, Tarek, Zino, Samer, Nassar, Ahmad HM, Vohra, Ravinder S, Kirkham, Amanda J, Pasquali, Sandro, Johnstone, Marianne, Spreadborough, Philip, Alderson, Derek, Fenwick, Stephen, Elmasry, Mohamed, Nunes, Quentin M, Kennedy, David, Khan, Raja Basit, Khan, Muhammad AS, Magee, Conor J, Jones, Steven M, Mason, Denise, Parappally, Ciny P, Mathur, Pawan, Saunders, Michael, Jamel, Sara, Haque, Samer Ul, Zafar, Sara, Shiwani, Muhammad Hanif, Samuel, Nehemiah, Dar, Farooq, Jackson, Andrew, Lovett, Bryony, Dindyal, Shiva, Winter, Hannah, Fletcher, Ted, Rahman, Saquib, Wheatley, Kevin, Nieto, Tom, Ayaani, Soofiyah, Youssef, Haney, Nijjar, Rajwinder S, Watkin, Helen, Naumann, David, Emesih, Sophie, Sarmah, Piyush B, Lee, Kathryn, Joji, Nikita, Lambert, Joel, Heath, Jonathan, Teasdale, Rebecca L, Weerasinghe, Chamindri, Needham, Paul J, Welbourn, Hannah, Forster, Luke, Finch, David, Blazeby, Jane M, Robb, William, McNair, Angus GK, Hrycaiczuk, Alex, Charalabopoulos, Alexandros, Kadirkamanathan, Sritharan, Tang, Cheuk-Bong, Jayanthi, Naga VG, Noor, Nigel, Dobbins, Brian, Cockbain, Andrew J, Nilsen-Nunn, April, de Siqueira, Jonathan, Pellen, Mike, Cowley, Jonathan B, Ho, Wei-Min, Miu, Victor, White, Timothy J, Hodgkins, Kathryn A, Kinghorn, Alison, Tutton, Matthew G, Al-Abed, Yahya A, Menzies, Donald, Ahmad, Anwar, Reed, Joanna, Khan, Shabuddin, Monk, David, Vitone, Louis J, Murtaza, Ghulam, Joel, Abraham, Brennan, Stephen, Shier, David, Zhang, Catherine, Yoganathan, Thusidaran, Robinson, Steven J, McCallum, Iain JD, Jones, Michael J, Elsayed, Mohammed, Tuck, Liz, Wayman, John, Carney, Kate, Aroori, Somaiah, Hosie, Kenneth B, Kimble, Adam, Bunting, David M, Fawole, Adeshina S, Basheer, Mohammed, Dave, Rajiv V, Sarveswaran, Janahan, Jones, Elinor, Kendal, Chris, Tilston, Michael P, Gough, Martin, Wallace, Tom, Singh, Shailendra, Downing, Justine, Mockford, Katherine A, Issa, Eyad, Shah, Nayab, Chauhan, Neal, Wilson, Timothy R, Forouzanfar, Amir, Wild, Jonathan RL, Nofal, Emma, Bunnell, Catherine, Madbak, Khaliel, Rao, Sudhindra TV, Devoto, Laurence, Siddiqi, Najaf, Khawaja, Zechan, Hewes, James C, Gould, Laura, Chambers, Alice, Rodriguez, Daniel Urriza, Sen, Gourab, Robinson, Stuart, Bartlett, Francis, Rae, David M, Stevenson, Thomas EJ, Sarvananthan, Kas, Dwerryhouse, Simon J, Higgs, Simon M, Old, Oliver J, Hardy, Thomas J, Shah, Reena, Hornby, Steve T, Keogh, Ken, Frank, Lucinda, Al-Akash, Musallam, Upchurch, Emma A, Frame, Richard J, Hughes, Michael, Jelley, Clare, Weaver, Simon, Roy, Sudipta, Sillo, Toritseju O, Galanopoulos, Giorgios, Cuming, Tamzin, Cunha, Pedro, Tayeh, Salim, Kaptanis, Sarantos, Heshaishi, Mohamed, Eisawi, Abdalla, Abayomi, Michael, Ngu, Wee Sing, Fleming, Katie, Bajwa, Dalvi S, Chitre, Vivek, Aryal, Kamal, Ferris, Paul, Silva, Michael, Lammy, Simon, Mohamed, Sarah, Khawaja, Amir, Hussain, Adnan, Ghazanfar, Mudassar A, Bellini, Maria Irene, Ebdewi, Hamdi, Elshaer, Mohamed, Gravante, Gianpiero, Drake, Benjamin, Ogedegbe, Arikoge, Mukherjee, Dipankar, Arhi, Chanpreet, Giwa, Lola, Iqbal, Nusrat, Watson, Nicholas F, Aggarwal, Smeer Kumar, Orchard, Philippa, Villatoro, Eduardo, Willson, Peter D, Mok, Kam Wa Jessica, Woodman, Thomas, Deguara, Jean, Garcea, Giuseppe, Babu, Benoy I, Dennison, AR, Malde, Deep, Lloyd, David, Satheesan, Steve, Al-Taan, Omer, Boddy, Alexander, Slavin, John P, Jones, Robert P, Ballance, Laura, Gerakopoulos, Stratos, Jambulingam, Periyathambi, Mansour, Sami, Sakai, Naomi, Acharya, Vikas, Sadat, Mohammed M, Karim, Lawen, Larkin, David, Amin, Khalid, Khan, Amarah, Law, Jennifer, Jamdar, Saurabh, Smith, Stella R, Sampat, Keerthika, O'shea, Kathryn M, Manu, Mangta, Asprou, Fotini M, Malik, Nabeela S, Chang, Jessica, Lewis, Michael, Roberts, Geoffrey P, Karavadra, Babu, Photi, Evangelos, Hewes, James, Rodriguez, Dan, O'Reilly, Derek A, Rate, Anthony J, Sekhar, Hema, Henderson, Lucy T, Starmer, Benjamin Z, Coe, Peter O, Tolofari, Sotonye, Barrie, Jenifer, Bashir, Gareth, Sloane, Jake, Madanipour, Suroosh, Halkias, Constantine, Trevatt, Alexander EJ, Borowski, David W, Hornsby, Jane, Courtney, Michael J, Virupaksha, Suvi, Seymour, Keith, Robinson, Sarah, Hawkins, Helen, Bawa, Sadiq, Gallagher, Paul V, Reid, Alistair, Wood, Peter, Finch, JG, Finch, J Guy, Parmar, J, Stirland, E, Gardner-Thorpe, James, Al-Muhktar, Ahmed, Peterson, Mark, Majeed, Ali, Bajwa, Farrukh M, Martin, Jack, Choy, Alfred, Tsang, Andrew, Pore, Naresh, Andrew, David R, Al-Khyatt, Waleed, Bhandari, Christopher Taylor Santosh, Chambers, Adam, Subramanium, Dhivya, Toh, Simon KC, Carter, Nicholas C, Tate, Sophie, Pearce, Belinda, Wainwright, Denise, Mercer, Stuart J, Knight, Benjamin, Vijay, Vardhini, Alagaratnam, Swethan, Sinha, Sidhartha, Khan, Shahab, El-Hasani, Shamsi S, Hussain, Abdulzahra A, Bhattacharya, Vish, Kansal, Nisheeth, Fasih, Tani, Jackson, Claire, Siddiqui, Midhat N, Chishti, Imran A, Fordham, Imogen J, Siddiqui, Zohaib, Bausbacher, Harald, Geogloma, Ileana, Gurung, Kabita, Tsavellas, George, Basynat, Pradeep, Shrestha, Ashish Kiran, Basu, Sanjoy, Harilingam, Alok Chhabra Mohan, Rabie, Mohamed, Akhtar, Mansoor, Kumar, Pradeep, Jafferbhoy, Sadaf F, Hussain, Najam, Raza, Soulat, Haque, Manzarul, Alam, Imran, Aseem, Rabiya, Patel, Shakira, Asad, Mehek, Booth, Michael I, Ball, William R, Wood, Christopher PJ, Pinho-Gomes, Ana C, Kausar, Ambareen, Obeidallah, Moh'd Rami, Varghase, Joseph, Lodhia, Joshil, Bradley, Donal, Rengifo, Carla, Lindsay, David, Gopalswamy, Sivakumar, Finlay, Ian, Wardle, Stacy, Bullen, Naomi, Iftikhar, Syed Yusuf, Awan, Altaf, Ahmed, Javed, Leeder, Paul, Fusai, Guiseppe, Bond-Smith, Giles, Psica, Alicja, Puri, Yogesh, Hou, David, Noble, Fergus, Szentpali, Karoly, Broadhurst, Jack, Date, Ravindra, Hossack, Martin R, Goh, Yan Li, Turner, Paul, Shetty, Vinutha, Riera, Manel, Macano, Christina AW, Sukha, Anisha, Preston, Shaun R, Hoban, Jennifer R, Puntis, Daniel J, Williams, Sophie V, Krysztopik, Richard, Kynaston, James, Batt, Jeremy, Doe, Matthew, Goscimski, Andrzej, Jones, Gareth H, Hall, Claire, Carty, Nick, Ahmed, Jamil, Panteleimonitis, Sofoklis, Gunasekera, Rohan T, Sheel, Andrea RG, Lennon, Hannah, Hindley, Caroline, Reddy, Marcus, Kenny, Ross, Elkheir, Natalie, McGlone, Emma R, Rajaganeshan, Rajasundaram, Hancorn, Kate, Hargreaves, Anita, Prasad, Raj, Longbotham, David A, Vijayanand, Dhakshinamoorthy, Wijetunga, Imeshi, Ziprin, Paul, Nicolay, Christopher R, Yeldham, Geoffrey, Read, Edward, Gossage, James A, Rolph, Rachel C, Ebied, Husam, Phull, Manraj, Khan, Mohammad A, Popplewell, Matthew, Kyriakidis, Dimitrios, Hussain, Anwar, Henley, Natasha, Packer, Jessica R, Derbyshire, Laura, Porter, Jonathan, Appleton, Shaun, Farouk, Marwan, Basra, Melvinder, Jennings, Neil A, Ali, Shahda, Kanakala, Venkatesh, Ali, Haythem, Lane, Risha, Dickson-Lowe, Richard, Zarsadias, Prizzi, Mirza, Darius, Puig, Sonia, Al Amari, Khalid, Vijayan, Deepak, Sutcliffe, Robert, Marudanayagam, Ravi, Hamady, Zayed, Prasad, Abheesh R, Patel, Abhilasha, Durkin, Damien, Kaur, Parminder, Bowen, Laura, Byrne, James P, Pearson, Katherine L, Delisle, Theo G, Davies, James, Tomlinson, Mark A, Johnpulle, Michelle A, Slawinski, Corinna, Macdonald, Andrew, Nicholson, James, Newton, Katy, Mbuvi, James, Farooq, Ansar, Mothe, Bhavani Sidhartha, Zafrani, Zakhi, Brett, Daniel, Francombe, James, Barnes, James, Cheung, Melanie, Al-Bahrani, Ahmed Z, Preziosi, Giuseppe, Urbonas, Tomas, Alberts, Justin, Mallik, Mekhlola, Patel, Krashna, Segaran, Ashvina, Doulias, Triantafyllos, Sufi, Pratik A, Yao, Caroline, Pollock, Sarah, Manzelli, Antonio, Wajed, Saj, Kourkulos, Michail, Pezzuto, Roberto, Wadley, Martin, Hamilton, Emma, Jaunoo, Shameen, Padwick, Robert, Sayegh, Mazin, Newton, Richard C, Hebbar, Madhusoodhana, Farag, Sameh F, Spearman, John, Hamdan, Mohammed F, D'Costa, Conrad, Blane, Christine, Giles, Mathew, Peter, Mark B, Hirst, Natalie A, Hossain, Tanvir, El-Dhuwaib, Arslan Pannu Yesar, Morrison, Tamsin EM, Taylor, Greg W, Thompson, Ronald LE, McCune, Ken, Loughlin, Paula, Lawther, Roger, Byrnes, Colman K, Simpson, Duncan J, Mawhinney, Abi, Warren, Conor, McKay, Damian, McIlmunn, Colin, Martin, Serena, MacArtney, Matthew, Diamond, Tom, Davey, Phil, Jones, Claire, Clements, Joshua M, Digney, Ruairi, Chan, Wei Ming, McCain, Stephen, Gull, Sadaf, Janeczko, Adam, Dorrian, Emmet, Harris, Andrew, Dawson, Suzanne, Johnston, Dorothy, McAree, Barry, Ghareeb, Essam, Thomas, George, Connelly, Martin, McKenzie, Stephen, Cieplucha, Krzysztos, Spence, Gary, Campbell, William, Hooks, Gareth, Bradley, Neil, Hill, Arnold DK, Cassidy, John T, Boland, Michael, Burke, Paul, Nally, Deirdre M, Khogali, Elmoataz, Shabo, Wael, Iskandar, Edrin, McEntee, Gerry P, O'Neill, Maeve A, Peirce, Colin, Lyons, Emma M, O'Sullivan, Adrian W, Thakkar, Rohan, Carroll, Paul, Ivanovski, Ivan, Balfe, Paul, Lee, Matthew, Winter, Des C, Kelly, Michael E, Hoti, Emir, Maguire, Donal, Karunakaran, Priyadarssini, Geoghegan, Justin G, McDermott, Frank, Martin, Sean T, Cross, Keith S, Cooke, Fiachra, Zeeshan, Saquib, Murphy, James O, Mealy, Ken, Mohan, Helen M, Nedujchelyn, Yuwaraja, Ullah, Muhammad Fahad, Ahmed, Irfan, Giovinazzo, Francesco, Milburn, James, Prince, Sarah, Brooke, Eleanor, Buchan, Joanna, Khalil, Ahmed M, Vaughan, Elizabeth M, Ramage, Michael I, Aldridge, Roland C, Gibson, Simon, Nicholson, Gary A, Vass, David G, Grant, Alan J, Holroyd, David J, Jones, M Angharad, Sutton, Cherith MLR, O'Dwyer, Patrick, Nilsson, Frida, Weber, Beatrix, Williamson, Tracey K, Lalla, Kushik, Bryant, Alice, Carter, C Ross, Forrest, Craig R, Hunter, David I, Nassar, Ahmad H, Orizu, Mavis N, Knight, Katrina, Qandeel, Haitham, Suttie, Stuart, Belding, Rowena, McClarey, Andrew, Boyd, Alan T, Guthrie, Graeme JK, Lim, Pei J, Luhmann, Andreas, Watson, Angus JM, Richards, Colin H, Nicol, Laura, Madurska, Marta, Harrison, Ewen, Boyce, Kathryn M, Roebuck, Amanda, Ferguson, Graeme, Pati, Pradeep, Wilson, Michael SJ, Dalgaty, Faith, Fothergill, Laura, Driscoll, Peter J, Mozolowski, Kirsty L, Banwell, Victoria, Bennett, Stephen P, Rogers, Paul N, Skelly, Brendan L, Rutherford, Claire L, Mirza, Ahmed K, Lazim, Taha, Lim, Henry CC, Duke, Diana, Ahmed, Talat, Beasley, William D, Wilkinson, Marc D, Maharaj, Geta, Malcolm, Cathy, Brown, Timothy H, Al-Sarireh, Bilal, Shingler, Guy M, Mowbray, Nicholas, Radwan, Rami, Morcous, Paul, Wood, Simon, Kadhim, Abbas, Stewart, Duncan J, Baker, Andrew L, Tanner, Nicola, Shenoy, Hrishikesh, Hafiz, Shazia, De Marchi, Joshua A, Singh-Ranger, Deepak, Hisham, Elzanati, Ainley, Paul, O'Neill, Stephen, Terrace, John, Napetti, Sara, Hopwood, Benjamin, Rhys, Thomas, Kanavati, Osama, Coats, Maria, Aleksandrov, Danail, Kallaway, Charlotte, Yahya, Salama, Templeton, Alexa, Trotter, Martin, Lo, Christina, Dhillon, Ajit, Heywood, Nick, Aawsaj, Yousif, Hamdan, Alhafidz, Reece-Bolton, Obuobi, McGuigan, Andrew, Shahin, Yousef, Aymon, Luther, Ali Alison, Nicholson, James A, Rajendran, Ilayaraja, Boal, Matthew, Ritchie, Judith, Grp, CholeS Study, Collaborative, West Midlands Res, Segaran, A, and Collaborative, West Midlands Research

Subjects
medicine.medical_specialty, medicine.medical_treatment, 030230 surgery, Article, 03 medical and health sciences, 0302 clinical medicine, Case mix index, Laparoscopic, length of stay, male, middle aged, postoperative complications, Medicine, Cholecystectomy, Prospective cohort study, Grading (education), humans, Laparoscopic cholecystectomy, business.industry, General surgery, adult, cholecystectomy, difficulty grading, laparoscopic, operative difficulty, surgery, aged, cholecystectomy, laparoscopic, conversion to open surgery, female, multivariate analysis, prospective studies, ROC curve, Difficulty grading, Cohort, 030211 gastroenterology & hepatology, Surgery, business, Operative difficulty, Grading scale, Abdominal surgery
Abstract

Background A reliable system for grading operative difficulty of laparoscopic cholecystectomy would standardise description of findings and reporting of outcomes. The aim of this study was to validate a difficulty grading system (Nassar scale), testing its applicability and consistency in two large prospective datasets. Methods Patient and disease-related variables and 30-day outcomes were identified in two prospective cholecystectomy databases: the multi-centre prospective cohort of 8820 patients from the recent CholeS Study and the single-surgeon series containing 4089 patients. Operative data and patient outcomes were correlated with Nassar operative difficultly scale, using Kendall’s tau for dichotomous variables, or Jonckheere–Terpstra tests for continuous variables. A ROC curve analysis was performed, to quantify the predictive accuracy of the scale for each outcome, with continuous outcomes dichotomised, prior to analysis. Results A higher operative difficulty grade was consistently associated with worse outcomes for the patients in both the reference and CholeS cohorts. The median length of stay increased from 0 to 4 days, and the 30-day complication rate from 7.6 to 24.4% as the difficulty grade increased from 1 to 4/5 (both p p Conclusion We have shown that an operative difficulty scale can standardise the description of operative findings by multiple grades of surgeons to facilitate audit, training assessment and research. It provides a tool for reporting operative findings, disease severity and technical difficulty and can be utilised in future research to reliably compare outcomes according to case mix and intra-operative difficulty.

Published
2018

21. JNK1 phosphorylation of SCG10 determines microtubule dynamics and axodendritic length

Author

Tararuk, Tatsiana, Ostman, Nina, Li, Wenrui, Bjorkblom, Benny, Padzik, Artur, Zdrojewska, Justyna, Hongisto, Vesa, Herdegen, Thomas, Konopka, Witold, Courtney, Michael J., and Coffey, Eleanor T.

Subjects
Microtubules -- Research, Morphogenesis -- Research, Biological sciences
Abstract

C-Jun N[H.sub.2]-terminal kinases (JNKs) are essential during brain development, when they regulate morphogenic changes involving cell movement and migration. In the adult, JNK determines neuronal cyto-architecture. To help uncover the molecular effectors for JNKs in these events, we affinity purified JNK-interacting proteins from brain. This revealed that the stathmin family microtubule-destabilizing proteins SCG10, SCLIP, RB3, and RB3' interact tightly with JNK. Furthermore, SCG10 is also phosphorylated by JNK in vivo on sites that regulate its microtubule depolymerizing activity, serines 62 and 73. SCG10-S73 phosphorylation is significantly decreased in JNK1-/- cortex, indicating that JNK1 phosphorylates SCG10 in developing forebrain. JNK phosphorylation of SCG10 determines axodendritic length in cerebrocortical cultures, and JNK site-phosphorylated SCG10 colocalizes with active JNK in embryonic brain regions undergoing neurite elongation and migration. We demonstrate that inhibition of cytoplasmic JNK and expression of SCG10-62A/73A both inhibited fluorescent tubulin recovery after photobleaching. These data suggest that JNK1 is responsible for regulation of SCG10 depolymerizing activity and neurite elongation during brain development.

Published
2006

30. Correction to: Utilisation of an operative difficulty grading scale for laparoscopic cholecystectomy (Surgical Endoscopy, (2018), 10.1007/s00464-018-6281-2)

Author

Griffiths, Ewen A., Hodson, James, Vohra, Ravi S., Marriott, Paul, Katbeh, Tarek, Zino, Samer, Nassar, Ahmad H. M., Vohra, Ravinder S., Kirkham, Amanda J., Pasquali, Sandro, Johnstone, Marianne, Spreadborough, Philip, Alderson, Derek, Fenwick, Stephen, Elmasry, Mohamed, Nunes, Quentin M., Kennedy, David, Khan, Raja Basit, Khan, Muhammad A. S., Magee, Conor J., Jones, Steven M., Mason, Denise, Parappally, Ciny P., Mathur, Pawan, Saunders, Michael, Jamel, Sara, Haque, Samer Ul, Zafar, Sara, Shiwani, Muhammad Hanif, Samuel, Nehemiah, Dar, Farooq, Jackson, Andrew, Lovett, Bryony, Dindyal, Shiva, Winter, Hannah, Fletcher, Ted, Rahman, Saquib, Wheatley, Kevin, Nieto, Tom, Ayaani, Soofiyah, Youssef, Haney, Nijjar, Rajwinder S., Watkin, Helen, Naumann, David, Emesih, Sophie, Sarmah, Piyush B., Lee, Kathryn, Joji, Nikita, Lambert, Joel, Heath, Jonathan, Teasdale, Rebecca L., Weerasinghe, Chamindri, Needham, Paul J., Welbourn, Hannah, Forster, Luke, Finch, David, Blazeby, Jane M., Robb, William, Mcnair, Angus G. K., Hrycaiczuk, Alex, Charalabopoulos, Alexandros, Kadirkamanathan, Sritharan, Tang, Cheuk-Bong, Jayanthi, Naga V. G., Noor, Nigel, Dobbins, Brian, Cockbain, Andrew J., Nilsen-Nunn, April, de Siqueira, Jonathan, Pellen, Mike, Cowley, Jonathan B., Wei-Min, Ho, Miu, Victor, White, Timothy J., Hodgkins, Kathryn A., Kinghorn, Alison, Tutton, Matthew G., Al-Abed, Yahya A., Menzies, Donald, Ahmad, Anwar, Reed, Joanna, Khan, Shabuddin, Monk, David, Vitone, Louis J., Murtaza, Ghulam, Joel, Abraham, Brennan, Stephen, Shier, David, Zhang, Catherine, Yoganathan, Thusidaran, Robinson, Steven J., Mccallum, Iain J. D., Jones, Michael J., Elsayed, Mohammed, Tuck, Liz, Wayman, John, Carney, Kate, Aroori, Somaiah, Hosie, Kenneth B., Kimble, Adam, Bunting, David M., Fawole, Adeshina S., Basheer, Mohammed, Dave, Rajiv V., Sarveswaran, Janahan, Jones, Elinor, Kendal, Chris, Tilston, Michael P., Gough, Martin, Wallace, Tom, Singh, Shailendra, Mockford, Justine Downing Katherine A., Issa, Eyad, Shah, Nayab, Chauhan, Neal, Wilson, Timothy R., Forouzanfar, Amir, Wild, Jonathan R. L., Nofal, Emma, Bunnell, Catherine, Madbak, Khaliel, Rao, Sudhindra T. V., Devoto, Laurence, Siddiqi, Najaf, Khawaja, Zechan, Hewes, James C., Gould, Laura, Chambers, Alice, Rodriguez, Daniel Urriza, Sen, Gourab, Robinson, Stuart, Bartlett, Francis, Rae, David M., Stevenson, Thomas E. J., Sarvananthan, Kas, Dwerryhouse, Simon J., Higgs, Simon M., Old, Oliver J., Hardy, Thomas J., Hornby, Reena Shah Steve T., Keogh, Ken, Frank, Lucinda, Al-Akash, Musallam, Upchurch, Emma A., Frame, Richard J., Hughes, Michael, Jelley, Clare, Weaver, Simon, Roy, Sudipta, Sillo, Toritseju O., Galanopoulos, Giorgios, Cuming, Tamzin, Cunha, Pedro, Tayeh, Salim, Kaptanis, Sarantos, Heshaishi, Mohamed, Eisawi, Abdalla, Abayomi, Michael, Ngu, Wee Sing, Fleming, Katie, Bajwa, Dalvir S., Chitre, Vivek, Aryal, Kamal, Ferris, Paul, Silva, Michael, Mohamed, Simon Lammy Sarah, Khawaja, Amir, Hussain, Adnan, Ghazanfar, Mudassar A., Bellini, Maria Irene, Ebdewi, Hamdi, Elshaer, Mohamed, Gravante, Gianpiero, Drake, Benjamin, Ogedegbe, Arikoge, Mukherjee, Dipankar, Arhi, Chanpreet, Iqbal, Lola Giwa Nusrat, Watson, Nicholas F., Aggarwal, Smeer Kumar, Orchard, Philippa, Villatoro, Eduardo, Willson, Peter D., Mok, Kam Wa Jessica, Woodman, Thomas, Deguara, Jean, Garcea, Giuseppe, Babu, Benoy I., Dennison, A. R., Malde, Deep, Lloyd, David, Satheesan, Steve, Al-Taan, Omer, Boddy, Alexander, Slavin, John P., Jones, Robert P., Ballance, Laura, Gerakopoulos, Stratos, Jambulingam, Periyathambi, Mansour, Sami, Sakai, Naomi, Acharya, Vikas, Sadat, Mohammed M., Karim, Lawen, Larkin, David, Amin, Khalid, Khan, Amarah, Law, Jennifer, Jamdar, Saurabh, Smith, Stella R., Sampat, Keerthika, O’Shea, Kathryn M., Manu, Mangta, Asprou, Fotini M., Malik, Nabeela S., Chang, Jessica, Lewis, Michael, Roberts, Geoffrey P., Karavadra, Babu, Photi, Evangelos, Hewes, James, Rodriguez, Dan, O’Reilly, Derek A., Rate, Anthony J., Sekhar, Hema, Henderson, Lucy T., Starmer, Benjamin Z., Coe, Peter O., Tolofari, Sotonye, Barrie, Jenifer, Bashir, Gareth, Sloane, Jake, Madanipour, Suroosh, Halkias, Constantine, Trevatt, Alexander E. J., Borowski, David W., Hornsby, Jane, Courtney, Michael J., Virupaksha, Suvi, Seymour, Keith, Robinson, Sarah, Hawkins, Helen, Bawa, Sadiq, Gallagher, Paul V., Reid, Alistair, Wood, Peter, Finch, J. G., Guy Finch, J., Parmar, J., Stirland, E., Gardner-Thorpe, James, Al-Muhktar, Ahmed, Peterson, Mark, Majeed, Ali, Bajwa, Farrukh M., Martin, Jack, Choy, Alfred, Tsang, Andrew, Pore, Naresh, Andrew, David R., Al-Khyatt, Waleed, Bhandari, Christopher Taylor Santosh, Chambers, Adam, Subramanium, Dhivya, Toh, Simon K. C., Carter, Nicholas C., Tate, Sophie, Pearce, Belinda, Wainwright, Denise, Mercer, Stuart J., Knight, Benjamin, Vijay, Vardhini, Alagaratnam, Swethan, Sinha, Sidhartha, Khan, Shahab, El-Hasani, Shamsi S., Hussain, Abdulzahra A., Bhattacharya, Vish, Kansal, Nisheeth, Fasih, Tani, Jackson, Claire, Siddiqui, Midhat N., Chishti, Imran A., Fordham, Imogen J., Siddiqui, Zohaib, Bausbacher, Harald, Geogloma, Ileana, Gurung, Kabita, Tsavellas, George, Basynat, Pradeep, Shrestha, Ashish Kiran, Basu, Sanjoy, Harilingam, Alok Chhabra Mohan, Rabie, Mohamed, Akhtar, Mansoor, Kumar, Pradeep, Jafferbhoy, Sadaf F., Hussain, Najam, Raza, Soulat, Haque, Manzarul, Alam, Imran, Aseem, Rabiya, Patel, Shakira, Asad, Mehek, Booth, Michael I., Ball, William R., Wood, Christopher P. J., Pinho-Gomes, Ana C., Kausar, Ambareen, Obeidallah, Moh’d Rami, Varghase, Joseph, Lodhia, Joshil, Bradley, Donal, Rengifo, Carla, Lindsay, David, Gopalswamy, Sivakumar, Finlay, Ian, Wardle, Stacy, Bullen, Naomi, Iftikhar, Syed Yusuf, Awan, Altaf, Ahmed, Javed, Leeder, Paul, Fusai, Guiseppe, Bond-Smith, Giles, Psica, Alicja, Puri, Yogesh, Hou, David, Noble, Fergus, Szentpali, Karoly, Broadhurst, Jack, Date, Ravindra, Hossack, Martin R., Goh, Yan Li, Turner, Paul, Shetty, Vinutha, Riera, Manel, Macano, Christina A. W., Sukha, Anisha, Preston, Shaun R., Hoban, Jennifer R., Puntis, Daniel J., Williams, Sophie V., Krysztopik, Richard, Kynaston, James, Batt, Jeremy, Doe, Matthew, Goscimski, Andrzej, Jones, Gareth H., Hall, Claire, Carty, Nick, Ahmed, Jamil, Panteleimonitis, Sofoklis, Gunasekera, Rohan T., Sheel, Andrea R. G., Lennon, Hannah, Hindley, Caroline, Reddy, Marcus, Kenny, Ross, Elkheir, Natalie, Mcglone, Emma R., Rajaganeshan, Rajasundaram, Hancorn, Kate, Hargreaves, Anita, Prasad, Raj, Longbotham, David A., Vijayanand, Dhakshinamoorthy, Wijetunga, Imeshi, Ziprin, Paul, Nicolay, Christopher R., Yeldham, Geoffrey, Read, Edward, Gossage, James A., Rolph, Rachel C., Ebied, Husam, Phull, Manraj, Khan, Mohammad A., Popplewell, Matthew, Kyriakidis, Dimitrios, Hussain, Anwar, Henley, Natasha, Packer, Jessica R., Derbyshire, Laura, Porter, Jonathan, Appleton, Shaun, Farouk, Marwan, Basra, Melvinder, Jennings, Neil A., Ali, Shahda, Kanakala, Venkatesh, Ali, Haythem, Lane, Risha, Dickson-Lowe, Richard, Zarsadias, Prizzi, Mirza, Darius, Puig, Sonia, Amari, Khalid Al, Vijayan, Deepak, Sutcliffe, Robert, Marudanayagam, Ravi, Hamady, Zayed, Prasad, Abheesh R., Patel, Abhilasha, Durkin, Damien, Kaur, Parminder, Bowen, Laura, Byrne, James P., Pearson, Katherine L., Delisle, Theo G., Davies, James, Tomlinson, Mark A., Johnpulle, Michelle A., Slawinski, Corinna, Macdonald, Andrew, Nicholson, James, Newton, Katy, Mbuvi, James, Farooq, Ansar, Mothe, Bhavani Sidhartha, Zafrani, Zakhi, Brett, Daniel, Francombe, James, Barnes, James, Cheung, Melanie, Al-Bahrani, Ahmed Z., Preziosi, Giuseppe, Urbonas, Tomas, Alberts, Justin, Mallik, Mekhlola, Patel, Krashna, Segaran, Ashvina, Doulias, Triantafyllos, Sufi, Pratik A., Yao, Caroline, Pollock, Sarah, Manzelli, Antonio, Wajed, Saj, Kourkulos, Michail, Pezzuto, Roberto, Wadley, Martin, Hamilton, Emma, Jaunoo, Shameen, Padwick, Robert, Sayegh, Mazin, Newton, Richard C., Hebbar, Madhusoodhana, Farag, Sameh F., Spearman, John, Hamdan, Mohammed F., D’Costa, Conrad, Blane, Christine, Giles, Mathew, Peter, Mark B., Hirst, Natalie A., Hossain, Tanvir, El-Dhuwaib, Arslan Pannu Yesar, Morrison, Tamsin E. M., Taylor, Greg W., Thompson, Ronald L. E., Mccune, Ken, Loughlin, Paula, Lawther, Roger, Byrnes, Colman K., Simpson, Duncan J., Mawhinney, Abi, Warren, Conor, Mckay, Damian, Mcilmunn, Colin, Martin, Serena, Macartney, Matthew, Diamond, Tom, Davey, Phil, Jones, Claire, Clements, Joshua M., Digney, Ruairi, Chan, Wei Ming, Mccain, Stephen, Gull, Sadaf, Janeczko, Adam, Dorrian, Emmet, Harris, Andrew, Dawson, Suzanne, Johnston, Dorothy, Mcaree, Barry, Ghareeb, Essam, Thomas, George, Connelly, Martin, Mckenzie, Stephen, Cieplucha, Krzysztos, Spence, Gary, Campbell, William, Hooks, Gareth, Bradley, Neil, Hill, Arnold D. K., Cassidy, John T., Boland, Michael, Burke, Paul, Nally, Deirdre M., Khogali, Elmoataz, Shabo, Wael, Iskandar, Edrin, Mcentee, Gerry P., O’Neill, Maeve A., Peirce, Colin, Lyons, Emma M., O’Sullivan, Adrian W., Thakkar, Rohan, Carroll, Paul, Ivanovski, Ivan, Balfe, Paul, Lee, Matthew, Winter, Des C., Kelly, Michael E., Hoti, Emir, Maguire, Donal, Karunakaran, Priyadarssini, Geoghegan, Justin G., Mcdermott, Frank, Martin, Sean T., Cross, Keith S., Cooke, Fiachra, Zeeshan, Saquib, Murphy, James O., Mealy, Ken, Mohan, Helen M., Nedujchelyn, Yuwaraja, Ullah, Muhammad Fahad, Ahmed, Irfan, Giovinazzo, Francesco, Milburn, James, Prince, Sarah, Brooke, Eleanor, Buchan, Joanna, Khalil, Ahmed M., Vaughan, Elizabeth M., Ramage, Michael I., Aldridge, Roland C., Gibson, Simon, Nicholson, Gary A., Vass, David G., Grant, Alan J., Holroyd, David J., Angharad Jones, M., Sutton, Cherith M. L. R., O’Dwyer, Patrick, Nilsson, Frida, Weber, Beatrix, Williamson, Tracey K., Lalla, Kushik, Bryant, Alice, Ross Carter, C., Forrest, Craig R., Hunter, David I., Nassar, Ahmad H., Orizu, Mavis N., Knight, Katrina, Qandeel, Haitham, Suttie, Stuart, Belding, Rowena, Mcclarey, Andrew, Boyd, Alan T., Guthrie, Graeme J. K., Lim, Pei J., Luhmann, Andreas, Watson, Angus J. M., Richards, Colin H., Nicol, Laura, Madurska, Marta, Harrison, Ewen, Boyce, Kathryn M., Roebuck, Amanda, Ferguson, Graeme, Pati, Pradeep, Wilson, Michael S. J., Dalgaty, Faith, Fothergill, Laura, Driscoll, Peter J., Mozolowski, Kirsty L., Banwell, Victoria, Bennett, Stephen P., Rogers, Paul N., Skelly, Brendan L., Rutherford, Claire L., Mirza, Ahmed K., Lazim, Taha, Lim, Henry C. C., Duke, Diana, Ahmed, Talat, Beasley, William D., Wilkinson, Marc D., Maharaj, Geta, Malcolm, Cathy, Brown, Timothy H., Al-Sarireh, Bilal, Shingler, Guy M., Mowbray, Nicholas, Radwan, Rami, Morcous, Paul, Wood, Simon, Kadhim, Abbas, Stewart, Duncan J., Baker, Andrew L., Tanner, Nicola, Shenoy, Hrishikesh, Hafiz, Shazia, De Marchi, Joshua A., Singh-Ranger, Deepak, Hisham, Elzanati, Ainley, Paul, John Terrace, Stephen O’Neill., Napetti, Sara, Hopwood, Benjamin, Rhys, Thomas, Downing, Justine, Kanavati, Osama, Coats, Maria, Aleksandrov, Danail, Kallaway, Charlotte, Yahya, Salama, Templeton, Alexa, Trotter, Martin, Christina, Lo, Dhillon, Ajit, Heywood, Nick, Aawsaj, Yousif, Hamdan, Alhafidz, Reece-Bolton, Obuobi, Mcguigan, Andrew, Shahin, Yousef, Aymon, Null, Luther, Ali Alison, Nicholson, James A., Rajendran, Ilayaraja, Boal, Matthew, and Ritchie, Judith

Subjects
Surgery
Published
2018

31. F193. Overexpression of NOS1AP in Dorsal Hippocampus and Medial Prefrontal Cortex Induces Schizophrenia-Related Phenotypic Changes

Author

Candemir, Esin, primary, Fattakhov, Nikolai, additional, Li, Li-Li, additional, Chen, Xufeng, additional, Esen, Dilhan, additional, Frerichs, Veronika, additional, Arshad, Saleha, additional, Grünewald, Lena, additional, O'Leary, Aet, additional, Slattery, David A., additional, von Engelhardt, Jakob, additional, Courtney, Michael J., additional, Reif, Andreas, additional, and Freudenberg, Florian, additional

Published
2018
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39. Preoperative risk factors for conversion from laparoscopic to open cholecystectomy: a validated risk score derived from a prospective U.K. database of 8820 patients

Author

Sutcliffe, Robert P., primary, Hollyman, Marianne, additional, Hodson, James, additional, Bonney, Glenn, additional, Vohra, Ravi S., additional, Griffiths, Ewen A., additional, Fenwick, Stephen, additional, Elmasry, Mohamed, additional, Nunes, Quentin, additional, Kennedy, David, additional, Khan, Raja B., additional, Khan, Muhammad A.S., additional, Magee, Conor J., additional, Jones, Steven M., additional, Mason, Denise, additional, Parappally, Ciny P., additional, Mathur, Pawan, additional, Saunders, Michael, additional, Jamel, Sara, additional, Haque, Samer U.l., additional, Zafar, Sara, additional, Shiwani, Muhammad H., additional, Samuel, Nehemiah, additional, Dar, Farooq, additional, Jackson, Andrew, additional, Lovett, Bryony, additional, Dindyal, Shiva, additional, Winter, Hannah, additional, Rahman, Saquib, additional, Wheatley, Kevin, additional, Nieto, Tom, additional, Ayaani, Soofiyah, additional, Youssef, Haney, additional, Nijjar, Rajwinder S., additional, Watkin, Helen, additional, Naumann, David, additional, Emeshi, Sophie, additional, Sarmah, Piyush B., additional, Lee, Kathryn, additional, Joji, Nikita, additional, Heath, Jonathan, additional, Teasdale, Rebecca L., additional, Weerasinghe, Chamindri, additional, Needham, Paul J., additional, Welbourn, Hannah, additional, Forster, Luke, additional, Finch, David, additional, Blazeby, Jane M., additional, Robb, William, additional, McNair, Angus G.K., additional, Hrycaiczuk, Alex, additional, Charalabopoulos, Alexandros, additional, Kadirkamanathan, Sritharan, additional, Tang, Cheuk-Bong, additional, Jayanthi, Naga V.G., additional, Noor, Nigel, additional, Dobbins, Brian, additional, Cockbain, Andrew J., additional, Nilsen-Nunn, April, additional, de Siqueira, Jonathan, additional, Pellen, Mike, additional, Cowley, Jonathan B., additional, Ho, Wei-Min, additional, Miu, Victor, additional, White, Timothy J., additional, Hodgkins, Kathryn A., additional, Kinghorn, Alison, additional, Tutton, Matthew G., additional, Al-Abed, Yahya A., additional, Menzies, Donald, additional, Ahmad, Anwar, additional, Reed, Joanna, additional, Khan, Shabuddin, additional, Monk, David, additional, Vitone, Louis J., additional, Murtaza, Ghulam, additional, Joel, Abraham, additional, Brennan, Stephen, additional, Shier, David, additional, Zhang, Catherine, additional, Yoganathan, Thusidaran, additional, Robinson, Steven J., additional, McCallum, Iain J.D., additional, Jones, Michael J., additional, Elsayed, Mohammed, additional, Tuck, Liz, additional, Wayman, John, additional, Carney, Kate, additional, Aroori, Somaiah, additional, Hosie, Kenneth B., additional, Kimble, Adam, additional, Bunting, David M., additional, Fawole, Adeshina S., additional, Basheer, Mohammed, additional, Dave, Rajiv V., additional, Sarveswaran, Janahan, additional, Jones, Elinor, additional, Kendal, Chris, additional, Tilston, Michael P., additional, Gough, Martin, additional, Wallace, Tom, additional, Singh, Shailendra, additional, Downing, Justine, additional, Mockford, Katherine A., additional, Issa, Eyad, additional, Shah, Nayab, additional, Chauhan, Neal, additional, Wilson, Timothy R., additional, Forouzanfar, Amir, additional, Wild, Jonathan R.L., additional, Nofal, Emma, additional, Bunnell, Catherine, additional, Madbak, Khaliel, additional, Rao, Sudhindra T.V., additional, Devoto, Laurence, additional, Siddiqi, Najaf, additional, Khawaja, Zechan, additional, Hewes, James C., additional, Gould, Laura, additional, Chambers, Alice, additional, Rodriguez, Daniel U., additional, Sen, Gourab, additional, Robinson, Stuart, additional, Bartlett, Francis, additional, Rae, David M., additional, Stevenson, Thomas E.J., additional, Sarvananthan, Kas, additional, Dwerryhouse, Simon J., additional, Higgs, Simon M., additional, Old, Oliver J., additional, Hardy, Thomas J., additional, Shah, Reena, additional, Hornby, Steve T., additional, Keogh, Ken, additional, Frank, Lucinda, additional, Al-Akash, Musallam, additional, Upchurch, Emma A., additional, Frame, Richard J., additional, Hughes, Michael, additional, Jelley, Clare, additional, Weaver, Simon, additional, Roy, Sudipta, additional, Sillo, Toritseju O., additional, Galanopoulos, Giorgios, additional, Cuming, Tamzin, additional, Cunha, Pedro, additional, Tayeh, Salim, additional, Kaptanis, Sarantos, additional, Heshaishi, Mohamed, additional, Eisawi, Abdalla, additional, Abayomi, Michael, additional, Ngu, Wee S., additional, Fleming, Katie, additional, Bajwa, Dalvir S., additional, Chitre, Vivek, additional, Aryal, Kamal, additional, Ferris, Paul, additional, Silva, Michael, additional, Lammy, Simon, additional, Mohamed, Sarah, additional, Khawaja, Amir, additional, Hussain, Adnan, additional, Ghazanfar, Mudassar A., additional, Bellini, Maria I., additional, Ebdewi, Hamdi, additional, Elshaer, Mohamed, additional, Gravante, Gianpiero, additional, Drake, Benjamin, additional, Ogedegbe, Arikoge, additional, Mukherjee, Dipankar, additional, Arhi, Chanpreet, additional, Giwa, Lola, additional, Iqbal, Nusrat, additional, Watson, Nicholas F., additional, Aggarwal, Smeer K., additional, Orchard, Philippa, additional, Villatoro, Eduardo, additional, Willson, Peter D., additional, Mok, Kam W.J., additional, Woodman, Thomas, additional, Deguara, Jean, additional, Garcea, Giuseppe, additional, Babu, Benoy I., additional, Dennison, Alistair R., additional, Malde, Deep, additional, Lloyd, David, additional, Slavin, John P., additional, Jones, Robert P., additional, Ballance, Laura, additional, Gerakopoulos, Stratos, additional, Jambulingam, Periyathambi, additional, Mansour, Sami, additional, Sakai, Naomi, additional, Acharya, Vikas, additional, Sadat, Mohammed M., additional, Karim, Lawen, additional, Larkin, David, additional, Amin, Khalid, additional, Khan, Amarah, additional, Law, Jennifer, additional, Jamdar, Saurabh, additional, Smith, Stella R., additional, Sampat, Keerthika, additional, O'shea, Kathryn M., additional, Manu, Mangta, additional, Asprou, Fotini M., additional, Malik, Nabeela S., additional, Chang, Jessica, additional, Johnstone, Marianne, additional, Lewis, Michael, additional, Roberts, Geoffrey P., additional, Karavadra, Babu, additional, Photi, Evangelos, additional, Hewes, James, additional, Rodriguez, Dan, additional, O'Reilly, Derek A., additional, Rate, Anthony J., additional, Sekhar, Hema, additional, Henderson, Lucy T., additional, Starmer, Benjamin Z., additional, Coe, Peter O., additional, Tolofari, Sotonye, additional, Barrie, Jenifer, additional, Bashir, Gareth, additional, Sloane, Jake, additional, Madanipour, Suroosh, additional, Halkias, Constantine, additional, Trevatt, Alexander E.J., additional, Borowski, David W., additional, Hornsby, Jane, additional, Courtney, Michael J., additional, Virupaksha, Suvi, additional, Seymour, Keith, additional, Robinson, Sarah, additional, Hawkins, Helen, additional, Bawa, Sadiq, additional, Gallagher, Paul V., additional, Reid, Alistair, additional, Wood, Peter, additional, Finch, Jonathan G., additional, Finch, J.Guy, additional, Parmar, Jitesh, additional, Stirland, Euan, additional, Gardner-Thorpe, James, additional, Al-Muhktar, Ahmed, additional, Peterson, Mark, additional, Majeed, Ali, additional, Bajwa, Farrukh M., additional, Martin, Jack, additional, Choy, Alfred, additional, Tsang, Andrew, additional, Pore, Naresh, additional, Andrew, David R., additional, Al-Khyatt, Waleed, additional, Santosh Bhandari, Christopher Taylor, additional, Chambers, Adam, additional, Subramanium, Dhivya, additional, Toh, Simon K.C., additional, Carter, Nicholas C., additional, Mercer, Stuart J., additional, Knight, Benjamin, additional, Vijay, Vardhini, additional, Alagaratnam, Swethan, additional, Sinha, Sidhartha, additional, Khan, Shahab, additional, El-Hasani, Shamsi S., additional, Hussain, Abdulzahra A., additional, Bhattacharya, Vish, additional, Kansal, Nisheeth, additional, Fasih, Tani, additional, Jackson, Claire, additional, Siddiqui, Midhat N., additional, Chishti, Imran A., additional, Fordham, Imogen J., additional, Siddiqui, Zohaib, additional, Bausbacher, Harald, additional, Geogloma, Ileana, additional, Gurung, Kabita, additional, Tsavellas, George, additional, Basynat, Pradeep, additional, Shrestha, Ashish K., additional, Basu, Sanjoy, additional, Chhabra, Alok, additional, Harilingam, Mohan, additional, Rabie, Mohamed, additional, Akhtar, Mansoor, additional, Kumar, Pradeep, additional, Jafferbhoy, Sadaf F., additional, Hussain, Najam, additional, Raza, Soulat, additional, Haque, Manzarul, additional, Alam, Imran, additional, Aseem, Rabiya, additional, Patel, Shakira, additional, Asad, Mehek, additional, Booth, Michael I., additional, Ball, William R., additional, Wood, Christopher P.J., additional, Pinho-Gomes, Ana C., additional, Kausar, Ambareen, additional, Obeidallah, Mohammed, additional, Varghase, Joseph, additional, Lodhia, Joshil, additional, Bradley, Donal, additional, Rengifo, Carla, additional, Lindsay, David, additional, Gopalswamy, Sivakumar, additional, Finlay, Ian, additional, Wardle, Stacy, additional, Bullen, Naomi, additional, Iftikhar, Syed Y., additional, Awan, Altaf, additional, Ahmed, Javed, additional, Leeder, Paul, additional, Fusai, Guiseppe, additional, Bond-Smith, Giles, additional, Psica, Alicja, additional, Puri, Yogesh, additional, Hou, David, additional, Noble, Fergus, additional, Szentpali, Karoly, additional, Broadhurst, Jack, additional, Date, Ravindra, additional, Hossack, Martin R., additional, Goh, Yan L., additional, Turner, Paul, additional, Shetty, Vinutha, additional, Riera, Manel, additional, Macano, Christina A.W., additional, Sukha, Anisha, additional, Preston, Shaun R., additional, Hoban, Jennifer R., additional, Puntis, Daniel J., additional, Williams, Sophie V., additional, Krysztopik, Richard, additional, Kynaston, James, additional, Batt, Jeremy, additional, Doe, Matthew, additional, Goscimski, Andrzej, additional, Jones, Gareth H., additional, Hall, Claire, additional, Carty, Nick, additional, Ahmed, Jamil, additional, Panteleimonitis, Sofoklis, additional, Gunasekera, Rohan T., additional, Sheel, Andrea R.G., additional, Lennon, Hannah, additional, Hindley, Caroline, additional, Reddy, Marcus, additional, Kenny, Ross, additional, Elkheir, Natalie, additional, McGlone, Emma R., additional, Rajaganeshan, Rajasundaram, additional, Hancorn, Kate, additional, Hargreaves, Anita, additional, Prasad, Raj, additional, Longbotham, David A., additional, Vijayanand, Dhakshinamoorthy, additional, Wijetunga, Imeshi, additional, Ziprin, Paul, additional, Nicolay, Christopher R., additional, Yeldham, Geoffrey, additional, Read, Edward, additional, Gossage, James A., additional, Rolph, Rachel C., additional, Ebied, Husam, additional, Phull, Manraj, additional, Khan, Mohammad A., additional, Popplewell, Matthew, additional, Kyriakidis, Dimitrios, additional, Hussain, Anwar, additional, Henley, Natasha, additional, Packer, Jessica R., additional, Derbyshire, Laura, additional, Porter, Jonathan, additional, Appleton, Shaun, additional, Farouk, Marwan, additional, Basra, Melvinder, additional, Jennings, Neil A., additional, Ali, Shahda, additional, Kanakala, Venkatesh, additional, Ali, Haythem, additional, Lane, Risha, additional, Dickson-Lowe, Richard, additional, Zarsadias, Prizzi, additional, Mirza, Darius, additional, Puig, Sonia, additional, Al Amari, Khalid, additional, Vijayan, Deepak, additional, Sutcliffe, Robert, additional, Marudanayagam, Ravi, additional, Hamady, Zayed, additional, Prasad, Abheesh R., additional, Patel, Abhilasha, additional, Durkin, Damien, additional, Kaur, Parminder, additional, Bowen, Laura, additional, Byrne, James P., additional, Pearson, Katherine L., additional, Delisle, Theo G., additional, Davies, James, additional, Tomlinson, Mark A., additional, Johnpulle, Michelle A., additional, Slawinski, Corinna, additional, Macdonald, Andrew, additional, Nicholson, James, additional, Newton, Katy, additional, Mbuvi, James, additional, Farooq, Ansar, additional, Mothe, Bhavani S., additional, Zafrani, Zakhi, additional, Brett, Daniel, additional, Francombe, James, additional, Spreadborough, Philip, additional, Barnes, James, additional, Cheung, Melanie, additional, Al-Bahrani, Ahmed Z., additional, Preziosi, Giuseppe, additional, Urbonas, Tomas, additional, Alberts, Justin, additional, Mallik, Mekhlola, additional, Patel, Krashna, additional, Segaran, Ashvina, additional, Doulias, Triantafyllos, additional, Sufi, Pratik A., additional, Yao, Caroline, additional, Pollock, Sarah, additional, Manzelli, Antonio, additional, Wajed, Saj, additional, Kourkulos, Michail, additional, Pezzuto, Roberto, additional, Wadley, Martin, additional, Hamilton, Emma, additional, Jaunoo, Shameen, additional, Padwick, Robert, additional, Sayegh, Mazin, additional, Newton, Richard C., additional, Hebbar, Madhusoodhana, additional, Farag, Sameh F., additional, Hebbar, Madhu, additional, Spearman, John, additional, Hamdan, Mohammed F., additional, D'Costa, Conrad, additional, Blane, Christine, additional, Giles, Mathew, additional, Peter, Mark B., additional, Hirst, Natalie A., additional, Hossain, Tanvir, additional, Pannu, Arslan, additional, El-Dhuwaib, Yesar, additional, Morrison, Tamsin E.M., additional, Taylor, Greg W., additional, Thompson, Ronald L.E., additional, McCune, Ken, additional, Loughlin, Paula, additional, Lawther, Roger, additional, Byrnes, Colman K., additional, Simpson, Duncan J., additional, Mawhinney, Abi, additional, Warren, Conor, additional, McKay, Damian, additional, McIlmunn, Colin, additional, Martin, Serena, additional, MacArtney, Matthew, additional, Diamond, Tom, additional, Davey, Phil, additional, Jones, Claire, additional, Clements, Joshua M., additional, Digney, Ruairi, additional, Chan, Wei M., additional, McCain, Stephen, additional, Gull, Sadaf, additional, Janeczko, Adam, additional, Dorrian, Emmet, additional, Harris, Andrew, additional, Dawson, Suzanne, additional, Johnston, Dorothy, additional, McAree, Barry, additional, Ghareeb, Essam, additional, Thomas, George, additional, Connelly, Martin, additional, McKenzie, Stephen, additional, Cieplucha, Krzysztos, additional, Spence, Gary, additional, Campbell, William, additional, Hooks, Gareth, additional, Bradley, Neil, additional, Hill, Arnold D.K., additional, Cassidy, John T., additional, Boland, Michael, additional, Burke, Paul, additional, Nally, Deirdre M., additional, Khogali, Elmoataz, additional, Shabo, Wael, additional, Iskandar, Edrin, additional, McEntee, Gerry P., additional, O'Neill, Maeve A., additional, Peirce, Colin, additional, Lyons, Emma M., additional, O'Sullivan, Adrian W., additional, Thakkar, Rohan, additional, Carroll, Paul, additional, Ivanovski, Ivan, additional, Balfe, Paul, additional, Lee, Matthew, additional, Winter, Des C., additional, Kelly, Michael E., additional, Hoti, Emir, additional, Maguire, Donal, additional, Karunakaran, Priyadarssini, additional, Geoghegan, Justin G., additional, Martin, Sean T., additional, Cross, Keith S., additional, Cooke, Fiachra, additional, Zeeshan, Saquib, additional, Murphy, James O., additional, Mealy, Ken, additional, Mohan, Helen M., additional, Nedujchelyn, Yuwaraja, additional, Ullah, Muhammad F., additional, Ahmed, Irfan, additional, Giovinazzo, Francesco, additional, Milburn, James, additional, Prince, Sarah, additional, Brooke, Eleanor, additional, Buchan, Joanna, additional, Khalil, Ahmed M., additional, Vaughan, Elizabeth M., additional, Ramage, Michael I., additional, Aldridge, Roland C., additional, Gibson, Simon, additional, Nicholson, Gary A., additional, Vass, David G., additional, Grant, Alan J., additional, Holroyd, David J., additional, Jones, Angharad, additional, Sutton, Cherith M.L.R., additional, O'Dwyer, Patrick, additional, Nilsson, Frida, additional, Weber, Beatrix, additional, Williamson, Tracey K., additional, Lalla, Kushik, additional, Bryant, Alice, additional, Carter, Ross, additional, Forrest, Craig R., additional, Hunter, David I., additional, Nassar, Ahmad H., additional, Orizu, Mavis N., additional, Knight, Katrina, additional, Qandeel, Haitham, additional, Suttie, Stuart, additional, Belding, Rowena, additional, McClarey, Andrew, additional, Boyd, Alan T., additional, Guthrie, Graeme J.K., additional, Lim, Pei J., additional, Luhmann, Andreas, additional, Watson, Angus J.M., additional, Richards, Colin H., additional, Nicol, Laura, additional, Madurska, Marta, additional, Harrison, Ewen, additional, Boyce, Kathryn M., additional, Roebuck, Amanda, additional, Ferguson, Graeme, additional, Pati, Pradeep, additional, Wilson, Michael S.J., additional, Dalgaty, Faith, additional, Fothergill, Laura, additional, Driscoll, Peter J., additional, Mozolowski, Kirsty L., additional, Banwell, Victoria, additional, Bennett, Stephen P., additional, Rogers, Paul N., additional, Skelly, Brendan L., additional, Rutherford, Claire L., additional, Mirza, Ahmed K., additional, Lazim, Taha, additional, Lim, Henry C.C., additional, Duke, Diana, additional, Ahmed, Talat, additional, Beasley, William D., additional, Wilkinson, Marc D., additional, Maharaj, Geta, additional, Malcolm, Cathy, additional, Brown, Timothy H., additional, Shingler, Guy M., additional, Mowbray, Nicholas, additional, Radwan, Rami, additional, Morcous, Paul, additional, Wood, Simon, additional, Kadhim, Abbas, additional, Stewart, Duncan J., additional, Baker, Andrew L., additional, Tanner, Nicola, additional, and Shenoy, Hrishikesh, additional

Published
2016
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41. ZLc002, a putative small-molecule inhibitor of nNOS interaction with NOSI AP, suppresses inflammatory nociception and chemotherapy-induced neuropathic pain and synergizes with paclitaxel to reduce tumor cell viability.

Author

Wan-Hung Lee, Hohmann, Andrea G., Carey, Lawrence M., Zhili Xu, Lai, Yvonne Y., Li-Li Li, and Courtney, Michael J.

Subjects
METHYL aspartate receptors, PROTEIN-protein interactions, NITRIC oxide, POSTSYNAPTIC density protein, DRUG development
Abstract

Elevated N-methyl-D-aspartate receptor activity contributes to central sensitization. Our laboratories and others recently reported that disrupting protein-protein interactions downstream of N-methyl-D-aspartate receptors suppresses pain. Specifically, disrupting binding between the enzyme neuronal nitric oxide synthase and either its upstream (postsynaptic density 95 kDa, PSD95) or downstream (e.g. nitric oxide synthase 1 adaptor protein, NOS1AP) protein partners suppressed inflammatory and/or neuropathic pain. However, the lack of a small-molecule neuronal nitric oxide synthase-NOS1AP inhibitor has hindered efforts to validate the therapeutic utility of disrupting the neuronal nitric oxide synthase-NOS1AP interface as an analgesic strategy. We, therefore, evaluated the ability of a putative small-molecule neuronal nitric oxide synthase-NOS1AP inhibitor ZLc002 to disrupt binding between neuronal nitric oxide synthase and NOS1AP using ex vivo, in vitro, and purified recombinant systems and asked whether ZLc002 would suppress inflammatory and neuropathic pain in vivo. In vitro, ZLc002 reduced co-immunoprecipitation of full-length NOS1AP and neuronal nitric oxide synthase in cultured neurons and in HEK293T cells co-expressing full-length neuronal nitric oxide synthase and NOS1AP. However, using a cellfree biochemical binding assay, ZLc002 failed to disrupt the in vitro binding between His-neuronal nitric oxide synthase1-299 and glutathione S-transferase-NOS1AP400-506, protein sequences containing the required binding domains for this protein-protein interaction, suggesting an indirect mode of action in intact cells. ZLc002 (4-10mg/kg i.p.) suppressed formalinevoked inflammatory pain in rats and reduced Fos protein-like immunoreactivity in the lumbar spinal dorsal horn. ZLc002 also suppressed mechanical and cold allodynia in a mouse model of paclitaxel-induced neuropathic pain. Anti-allodynic efficacy was sustained for at least four days of once daily repeated dosing. ZLc002 also synergized with paclitaxel when administered in combination to reduce breast (4T1) or ovarian (HeyA8) tumor cell line viability but did not alter tumor cell viability without paclitaxel. Our results verify that ZLc002 disrupts neuronal nitric oxide synthase-NOS1AP interaction in intact cells and demonstrate, for the first time, that systemic administration of a putative small-molecule inhibitor of neuronal nitric oxide synthase-NOS1AP suppresses inflammatory and neuropathic pain. [ABSTRACT FROM AUTHOR]

Published
2018
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43. Unexpected Heterodivalent Recruitment of NOS1AP to nNOS Reveals Multiple Sites for Pharmacological Intervention in Neuronal Disease Models

Author

Li, Lili, De Mera, Raquel M. Melero-Fernandez, Chen, Jia, Ba, Wei, Kasri, Nael Nadif, Zhang, Mingjie, Courtney, Michael J., Li, Lili, De Mera, Raquel M. Melero-Fernandez, Chen, Jia, Ba, Wei, Kasri, Nael Nadif, Zhang, Mingjie, and Courtney, Michael J.

Abstract

The protein NOS1AP/CAPON mediates signaling from a protein complex of NMDA receptor, PSD95 and nNOS. The only stroke trial for neuroprotectants that showed benefit to patients targeted this ternary complex. NOS1AP/nNOS interaction regulates small GTPases, iron transport, p38MAPK-linked excitotoxicity, and anxiety. Moreover, the nos1ap gene is linked to disorders from schizophrenia, post-traumatic stress disorder, and autism to cardiovascular disorders and breast cancer. Understanding protein interactions required for NOS1AP function, therefore, has broad implications for numerous diseases. Here we show that the interaction of NOS1AP with nNOS differs radically from the classical PDZ docking assumed to be responsible. The NOS1AP PDZ motif does not bind nNOS as measured by multiple methods. In contrast, full-length NOS1AP forms an unusually stable interaction with nNOS. We mapped the discrepancy between full-length and C-terminal PDZ motif to a novel internal region we call the ExF motif. The C-terminal PDZ motif, although neither sufficient nor necessary for binding, nevertheless promotes the stability of the complex. It therefore potentially affects signal transduction and suggests that functional interaction of nNOS with NOS1AP might be targetable at two distinct sites. We demonstrate that excitotoxic pathways can be regulated, in cortical neuron and organotypic hippocampal slice cultures from rat, either by the previously described PDZ ligand TAT-GESV or by the ExF motif-bearing region of NOS1AP, even when lacking the critical PDZ residues as long as the ExF motif is intact and not mutated. This previously unrecognized heterodivalent interaction of nNOS with NOS1AP may therefore provide distinct opportunities for pharmacological intervention in NOS1AP-dependent signaling and excitotoxicity.

Published
2015

47. Phosphorylation of SCG10/stathmin-2 determines multipolar stage exit and neuronal migration rate

Author

Westerlund, Nina, Zdrojewska, Justyna, Padzik, Artur, Komulainen, Emilia, Björkblom, Benny, Rannikko, Emmy, Tararuk, Tanya, Garcia-Frigola, Cristina, Sandholm, Jouko, Nguyen, Laurent, Kallunki, Tuula, Courtney, Michael J, Coffey, Eleanor T, Westerlund, Nina, Zdrojewska, Justyna, Padzik, Artur, Komulainen, Emilia, Björkblom, Benny, Rannikko, Emmy, Tararuk, Tanya, Garcia-Frigola, Cristina, Sandholm, Jouko, Nguyen, Laurent, Kallunki, Tuula, Courtney, Michael J, and Coffey, Eleanor T

Abstract

Cell migration is the consequence of the sum of positive and negative regulatory mechanisms. Although appropriate migration of neurons is a principal feature of brain development, the negative regulatory mechanisms remain obscure. We found that JNK1 was highly active in developing cortex and that selective inhibition of JNK in the cytoplasm markedly increased both the frequency of exit from the multipolar stage and radial migration rate and ultimately led to an ill-defined cellular organization. Moreover, regulation of multipolar-stage exit and radial migration in Jnk1−/− (also known as Mapk8) mice, resulted from consequential changes in phosphorylation of the microtubule regulator SCG10 (also called stathmin-2). Expression of an SCG10 mutant that mimics the JNK1-phosphorylated form restored normal migration in the brains of Jnk1−/− mouse embryos. These findings indicate that the phosphorylation of SCG10 by JNK1 is a fundamental mechanism that governs the transition from the multipolar stage and the rate of neuronal cell movement during cortical development.

Published
2011
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49. Constitutively Active Cytoplasmic c-Jun N-Terminal Kinase 1 Is a Dominant Regulator of Dendritic Architecture: Role of Microtubule-Associated Protein 2 as an Effector

Author

Björkblom, Benny, Östman, Nina, Hongisto, Vesa, Komarovski, Vladislav, Filén, Jan-Jonas, Nyman, Tuula A., Kallunki, Tuula, Courtney, Michael J., Coffey, Eleanor T., Björkblom, Benny, Östman, Nina, Hongisto, Vesa, Komarovski, Vladislav, Filén, Jan-Jonas, Nyman, Tuula A., Kallunki, Tuula, Courtney, Michael J., and Coffey, Eleanor T.

Abstract

Normal functioning of the nervous system requires precise regulation of dendritic shape and synaptic connectivity. Here, we report a severe impairment of dendritic structures in the cerebellum and motor cortex of c-Jun N-terminal kinase 1 (JNK1)-deficient mice. Using an unbiased screen for candidate mediators, we identify the dendrite-specific high-molecular-weight microtubule-associated protein 2 (MAP2) as a JNK substrate in the brain. We subsequently show that MAP2 is phosphorylated by JNK in intact cells and that MAP2 proline-rich domain phosphorylation is decreased in JNK1-/- brain. We developed compartment-targeted JNK inhibitors to define whether a functional relationship exists between the physiologically active, cytosolic pool of JNK and dendritic architecture. Using these, we demonstrate that cytosolic, but not nuclear, JNK determines dendritic length and arbor complexity in cultured neurons. Moreover, we confirm that MAP2-dependent process elongation is enhanced after activation of JNK. Using JNK1-/- neurons, we reveal a dominant role for JNK1 over ERK in regulating dendritic arborization, whereas ERK only regulates dendrite shape under conditions in which JNK activity is low (JNK1-/- neurons). These results reveal a novel antagonism between JNK and ERK, potentially providing a mechanism for fine-tuning the dendritic arbor. Together, these data suggest that JNK phosphorylation of MAP2 plays an important role in defining dendritic architecture in the brain.

Published
2005
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50. c-Jun N-Terminal Kinase Phosphorylation of MARCKSL1 Determines Actin Stability and Migration in Neurons and in Cancer Cells

Author

Björkblom, Benny, primary, Padzik, Artur, additional, Mohammad, Hasan, additional, Westerlund, Nina, additional, Komulainen, Emilia, additional, Hollos, Patrik, additional, Parviainen, Lotta, additional, Papageorgiou, Anastassios C., additional, Iljin, Kristiina, additional, Kallioniemi, Olli, additional, Kallajoki, Markku, additional, Courtney, Michael J., additional, Mågård, Mats, additional, James, Peter, additional, and Coffey, Eleanor T., additional

Published
2012
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