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106 results on '"Coupek, J."'

6. Binding of C1q and DNA to Support Materials by Means of A new Coupling Procedure

Author

Schößler, W., Hiepe, P., Coupek, J., and Apostoloff, E.

Abstract

Since several years matrix-bound biologically active substances are widely used in biosciences, biotechnology and medicine. We are presenting a simple and inexpensive activation procedure for support materials which allows stable binding of C1q and DNA. We could not found a remarkable leakage of the bound ligands. In connection with the good biocompatiblity of our procedure, both are important for the application in extracorporeal immunoadsorption. The method is technologically simple and represents an alternative to the activation methods applied so far.

Published
1990
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7. Mechanism of anionic polymerization of acrylates and methacrylates

Author

Báca, J., Lochmann, L., Juzl, K., Coupek, J., and Lím, D.

Abstract

The mechanism of anionic polymerization of acrylates and methacrylates was studied by differential thermometry. The course of the temperature curves after the addition of the initiator to the monomer was analyzed. As initiator, organolithium and sodium compounds as well as lithium, sodium, and potassium tert. alkoxides were used. It was found that in case of methacrylates the lifetime of growing chains is according to the type of the initiator and monomer from seconds to about 1 min. In the case of lithium alkoxide, formation of active centers is a relatively slow process, different from potassium alkoxide, where the whole process resembles polymerization by organometallic compounds. Sodium alkoxide initiator evidently causes a very complicated process. After the region of acceleration is reached, polymerization resembles potassium alkoxide initiation. In the case of lithium compounds the high isotactic portion depends on the alkoxide present, whereas with sodium compounds, atactic polymer is normally obtained. Potassium alkoxide leads to slightly increased syndiotactic portion.

Published
1967
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8. Isolation of immunoreactive components from experimental and human tumour tissues and serums by high-performance gel chromatography

Author

Jandová A, Motycka K, Pezlarová J, Coupek J, and Sanitrák J

Subjects
Male, Chromatography, Elution, Chemistry, Immunochemistry, Leukocyte Adherence Inhibition Test, Clinical state, General Chemistry, Neoplasms, Experimental, Neoplasm Proteins, Gel permeation chromatography, Mice, Chromatography, Gel, Animals, Humans, Female
Abstract

A method for the rapid separation of proteinous fractions by high-performance gel chromatography was described. Homogenates from tumorous and healthy tissues and blood were eluted by saline on a column packed with rigid hydrophilic macroporous particles of O-glucosa—Spheron 300. Fractions were collected and subjected to further analyses. Their antigenic activity was determined by the leucocyte adherence inhibition test method. For the specific immunoactive fractions a dependence of leucocyte adherence inhibition test values on the clinical state of sample donators has been found.

Published
1978

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