35 results on '"Couet D"'
Search Results
2. Intérêt de la biologie moléculaire dans le diagnostic rapide de la trisomie 21 et des aneuploïdies les plus fréquentes
- Author
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Fauret, A.-L., Bilan, F., Patri, S., Couet, D., Marechaud, M., Pierre, F., Gilbert-Dussardier, B., and Kitzis, A.
- Published
- 2009
- Full Text
- View/download PDF
3. Recurrent ctb(7)(q31.3) and possible laminin involvement in a neonatal cutis laxa with a Marfan phenotype
- Author
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Bonneau, D., Huret, J. L., Godeau, G., Couet, D., Putterman, M., Tanzer, J., Babin, P., and Larrègue, M.
- Published
- 1991
- Full Text
- View/download PDF
4. Effects of physical forcing on COastal ZOoplankton community structure: study of the unusual case of a MEDiterranean ecosystem under strong tidal influence (Project COZOMED-MERMEX)
- Author
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Pagano, Marc, Arfi, Robert, Atoui, A., Ayadi, H., Bejaoui, B., Bhairy, Nagib, Barraj, N., Belhassen, Malika, Benismail, S., Benkacem, M.y, Blanchot, Jean, Čanković, Milan, Carlotti, Francois, Chevalier, Cristele, Ciglenecki-Jusic, I., Couet, D., Daly Yahia, Mohamed Néjib, Dammak, L., Devenon, Jean-Luc, Drira, Zaher, Hamza, A., Kmia, S., Makhlouf, N., Mahfoudi, Mohamed, Moncef, M., Sammari, Cherif, Smeti, Houssem, Zouari, Amel, Institut méditerranéen d'océanologie (MIO), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS), and Institut National des Sciences et Technologies de la Mer [Salammbô] (INSTM)
- Subjects
M. Mahfoudi (2) ,N. Daly Yahia (3) ,J. Blanchot (1) ,J.-L. Devenon (1) ,M.Y Benkacem (2) ,M. Cankovic(5) ,C. Sammari (2) ,L. Dammak (2) ,H. Ayadi (6) ,M. Belhassen (2) ,I Ciglenecki-Jusic (5) ,D. Couet (1) ,H. Smeti (2) ,C. Chevalier (1) ,M. Moncef (4) ,N. Makhlouf (3) ,N. Barraj (2) ,S. Kmia (6) ,F. Carlotti (1) ,[SDU.OCEAN]Sciences of the Universe [physics]/Ocean, Atmosphere ,M. Pagano (1) ,N. Bhairy (1) ,A. Atoui (2) ,A. Zouari (2) ,A. Hamza (2) ,Groupe COZOMED: R. Arfi (1) ,B. Bejaoui (1) ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology ,S. Benismail (2) ,Z. Drira (6) - Abstract
International audience; The COZOMED-MERMEX project aims at understanding how hydrodynamic forcing (currents, tides, winds)combine with anthropogenic forcing and climate to affect the variability of coastal Mediterranean zooplanktoncommunities under contrasting tidal influence. This study includes (i) a zero state of knowledge via a literaturereview of existing data and (ii) a case study on the system Boughrara lagoon - Gulf of Gabes. This ecosystemgives major services for Tunisia (about 65% of national fish production) but is weakened by its situation in aheavily anthropized area and under influence of urban, industrial and agricultural inputs. Besides this regionis subject to specific climate forcing (Sahelian winds, scorching heat, intense evaporation, flooding) whichpossible changes will be considered. The expected issues are (i) to improve our knowledge of hydrodynamicforcing on zooplankton and ultimately on the functioning of coastal Mediterranean ecosystems impacted byanthropogenic and climatic effects and (ii) to elaborate management tools to help preserving good ecologicalstatus of these ecosystems: hydrodynamic circulation model, mapping of isochrones of residence times, mappingof the areas of highest zooplankton abundances (swarms), and sensitive areas, etc. This project strengthensexisting scientific collaborations within the MERMEX program (The MerMex Group, 2011) and in the frameof an international joint laboratory (COSYS-Med) created in 2014. A first field mulidisciplinary campaign wasperformed in October 2016. The strategy combined measurements of sea level and currents (mooring of ADCP,Argonaute and tidal gauges), hydrological description of water masses (horizontal and vertical transect with aMinibat equipped with CTD, fluorescence and turbidity sensors) and discrete sampling of nutrients, DOC, POC,pico, nano, microphytoplankton and mesozooplankton. The first results allow a description of water currents andshows a good coupling between tidal cycles (ebb-flood and spring tide neap tide) and the dynamics of planktoniccompartments in the lagoon.
- Published
- 2017
5. Effects of physical forcing on COastal ZOoplankton community structure: study of the unusual case of a MEDiterranean ecosystem under strong tidal influence (Project COZOMED-MERMEX)
- Author
-
Marc, Pagano, ARFI, Robert, Atoui, A., Ayadi, H., Bejaoui, B., Bhairy, Nagib, Barraj, N., Belhassen, Malika, Benismail, S., Benkacem, M.Y, BLANCHOT, Jean, Čanković, Milan, CARLOTTI, Francois, CHEVALIER, Cristele, Ciglenecki-Jusic, I., Couet, D., Daly Yahia, Mohamed Néjib, Dammak, L., Devenon, Jean-Luc, Drira, Zaher, Hamza, A., Kmia, S., Makhlouf, N., Mahfoudi, Mohamed, Moncef, M., Sammari, Cherif, Smeti, Houssem, Zouari Bellaaj, Amel, PAGANO, Marc, Team, Groupe, Institut méditerranéen d'océanologie (MIO), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Toulon (UTLN), and Institut National des Sciences et Technologies de la Mer [Salammbô] (INSTM)
- Subjects
M. Mahfoudi (2) ,N. Daly Yahia (3) ,J. Blanchot (1) ,J.-L. Devenon (1) ,M.Y Benkacem (2) ,M. Cankovic(5) ,C. Sammari (2) ,L. Dammak (2) ,H. Ayadi (6) ,M. Belhassen (2) ,I Ciglenecki-Jusic (5) ,D. Couet (1) ,H. Smeti (2) ,C. Chevalier (1) ,M. Moncef (4) ,N. Makhlouf (3) ,N. Barraj (2) ,S. Kmia (6) ,F. Carlotti (1) ,[SDU.OCEAN]Sciences of the Universe [physics]/Ocean, Atmosphere ,M. Pagano (1) ,N. Bhairy (1) ,A. Atoui (2) ,A. Zouari (2) ,A. Hamza (2) ,Groupe COZOMED: R. Arfi (1) ,B. Bejaoui (1) ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology ,S. Benismail (2) ,Z. Drira (6) - Abstract
International audience; The COZOMED-MERMEX project aims at understanding how hydrodynamic forcing (currents, tides, winds)combine with anthropogenic forcing and climate to affect the variability of coastal Mediterranean zooplanktoncommunities under contrasting tidal influence. This study includes (i) a zero state of knowledge via a literaturereview of existing data and (ii) a case study on the system Boughrara lagoon - Gulf of Gabes. This ecosystemgives major services for Tunisia (about 65% of national fish production) but is weakened by its situation in aheavily anthropized area and under influence of urban, industrial and agricultural inputs. Besides this regionis subject to specific climate forcing (Sahelian winds, scorching heat, intense evaporation, flooding) whichpossible changes will be considered. The expected issues are (i) to improve our knowledge of hydrodynamicforcing on zooplankton and ultimately on the functioning of coastal Mediterranean ecosystems impacted byanthropogenic and climatic effects and (ii) to elaborate management tools to help preserving good ecologicalstatus of these ecosystems: hydrodynamic circulation model, mapping of isochrones of residence times, mappingof the areas of highest zooplankton abundances (swarms), and sensitive areas, etc. This project strengthensexisting scientific collaborations within the MERMEX program (The MerMex Group, 2011) and in the frameof an international joint laboratory (COSYS-Med) created in 2014. A first field mulidisciplinary campaign wasperformed in October 2016. The strategy combined measurements of sea level and currents (mooring of ADCP,Argonaute and tidal gauges), hydrological description of water masses (horizontal and vertical transect with aMinibat equipped with CTD, fluorescence and turbidity sensors) and discrete sampling of nutrients, DOC, POC,pico, nano, microphytoplankton and mesozooplankton. The first results allow a description of water currents andshows a good coupling between tidal cycles (ebb-flood and spring tide neap tide) and the dynamics of planktoniccompartments in the lagoon.
- Published
- 2017
6. Le syndrome de Williams-Beuren : une approche pluridisciplinaire
- Author
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Lacroix, A., primary, Pezet, M., additional, Capel, A., additional, Bonnet, D., additional, Hennequin, M., additional, Jacob, M.-P., additional, Bricca, G., additional, Couet, D., additional, Faury, G., additional, Bernicot, J., additional, and Gilbert-Dussardier, B., additional
- Published
- 2009
- Full Text
- View/download PDF
7. Recurrent ctb(7)(q31.3) and possible laminin involvement in a neonatal cutis laxa with a Marfan phenotype
- Author
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Dominique Bonneau, P. Babin, Joseph Tanzer, M. Larrègue, J. L. Huret, M. Putterman, Couet D, and G. Godeau
- Subjects
Marfan syndrome ,Systemic disease ,Pathology ,medicine.medical_specialty ,Cutis Laxa ,Marfan Syndrome ,Immunoenzyme Techniques ,Laminin ,Genetics ,medicine ,Humans ,Diaphragmatic hernia ,Genetics (clinical) ,Cells, Cultured ,biology ,Infant ,Anatomy ,medicine.disease ,Connective tissue disease ,Molecular medicine ,Phenotype ,Karyotyping ,biology.protein ,Female ,Chromosomes, Human, Pair 7 ,Cutis laxa - Abstract
A 6-week-old girl presented with cutis laxa, emphysema, heart anomalies and a diaphragmatic hernia. She died at 22 weeks. A recurrent ctb(7)(q31.3) was found and the laminin gene was suspected to be involved in the disease. Anti-human laminin antiserum showed that this protein was absent from the skin. This case, together with 17 other similar cases, could represent a new type of connective tissue disease.
- Published
- 1991
8. INV(12) (p13q11) and dicentric chromosomes in a secondary leukaemia with basophilia
- Author
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Jl, Huret, Brizard A, François Guilhot, Couet D, Schoenwald M, Jr, Savage, and Tanzer J
- Subjects
Chromosome Aberrations ,Chromosomes, Human, Pair 12 ,Skin Neoplasms ,Leukemia, Myelomonocytic, Chronic ,Vinblastine ,Basophils ,Dacarbazine ,Neoplasms, Multiple Primary ,Methotrexate ,Antineoplastic Combined Chemotherapy Protocols ,Chromosome Inversion ,Chromosomes, Human, Pair 5 ,Humans ,Chromosomes, Human, Pair 6 ,Female ,Streptonigrin ,Blast Crisis ,Melanoma ,Thiotepa ,Aged ,Chromosomes, Human, Pair 8 - Abstract
We report on a case of secondary myelodysplasia with basophilia. A karyotype showed 1) the involvement of 12p, which should be related to the basophilia and/or to previous drug exposure, and 2) the unexpected presence of three different dicentric chromosomes.
- Published
- 1990
9. Obesity
- Author
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MARLIN, S., primary, COUET, D., additional, LACOMBE, D., additional, CESSANS, C., additional, and BONNEAU, D., additional
- Published
- 1994
- Full Text
- View/download PDF
10. Obesity
- Author
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Didier Lacombe, Couet D, Dominique Bonneau, Sandrine Marlin, and Cessans C
- Subjects
External genital hypoplasia ,Pediatrics ,medicine.medical_specialty ,business.industry ,media_common.quotation_subject ,WAGR syndrome ,macromolecular substances ,General Medicine ,Severe obesity ,medicine.disease ,Obesity ,Pathology and Forensic Medicine ,Aniridia ,Feature (computer vision) ,Pediatrics, Perinatology and Child Health ,medicine ,Girl ,Anatomy ,business ,Genetics (clinical) ,media_common - Abstract
A 6-year-old girl with del(11)(p14p12) is reported. This girl has the multiple congenital anomalies that defines the WAGR syndrome (aniridia, external genital hypoplasia and severe mental retardation). She has, in addition, very severe obesity (+10 SD) which is not a feature usually described with WAGR association.
- Published
- 1994
11. A two-step t(4;der(15)) t(15;17) complex translocation in an acute promyelocytic leukaemia and review of the literature
- Author
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Joseph Tanzer, François Guilhot, J. L. Huret, André Brizard, and Couet D
- Subjects
Male ,Cancer Research ,medicine.medical_specialty ,Two step ,Chromosomal translocation ,T-15 ,Biology ,Translocation, Genetic ,Chromosome 15 ,hemic and lymphatic diseases ,medicine ,Humans ,Aged ,Genetics ,Chromosomes, Human, Pair 15 ,Cytogenetics ,Hematology ,Molecular biology ,Chromosome 17 (human) ,Leukemia, Myeloid, Acute ,Chromosome 4 ,Oncology ,Karyotyping ,Acute promyelocytic leukaemia ,Chromosomes, Human, Pair 4 ,Chromosomes, Human, Pair 17 - Abstract
We report the first case of a two step complex translocation in acute promyelocytic leukaemia and discuss the chromosomal mechanism involved.
- Published
- 1987
12. Malignant histiocytosis: a specific t(2;5)(p23;q35) translocation? Review of the literature
- Author
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P. Babin, Elisabeth Benz-lemoine, François Guilhot, Couet D, André Brizard, J. L. Huret, and Joseph Tanzer
- Subjects
medicine.medical_specialty ,Pathology ,Adolescent ,Malignant histiocytosis ,Breakpoint ,Immunology ,Cytogenetics ,Chromosomal translocation ,Disease ,Cell Biology ,Hematology ,Biology ,medicine.disease ,Biochemistry ,Subclass ,Translocation, Genetic ,Chromosomes, Human, Pair 2 ,Karyotyping ,medicine ,Chromosomes, Human, Pair 5 ,Humans ,Female ,Histiocytic Sarcoma - Abstract
In this paper, the investigators report a well documented case of malignant histiocytosis (MH) with a t(2;5)(p23;q35) translocation. A breakpoint in 5q35 appears to be specific, either for the disease or for a subclass of the disease. Additional cases of MH with cytogenetics are needed. This will help to determine if one class of MH or several subclasses can be defined by cytogenetic anomaly(ies).
- Published
- 1988
13. Lujan-fryns syndrome (X-linked mental retardation with marfanoid habitus): Report of three cases and review
- Author
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Lacombe D, Bonneau D, Alain VERLOES, Couet D, Koulischer L, and Battin J
- Subjects
Male ,X Chromosome ,Adolescent ,Foot Deformities, Congenital ,Genetic Linkage ,Skull ,Syndrome ,Facial Bones ,Marfan Syndrome ,Intellectual Disability ,Arm ,Humans ,Abnormalities, Multiple ,Hand Deformities, Congenital ,Growth Disorders - Abstract
We report on three further cases of mildly retarded patients with marfanoid habitus and a pattern of minor anomalies. These patients are likely to be affected with the Lujan-Fryns syndrome. We have reviewed twenty cases from the literature for a better delineation of this newly recognized disorder.
14. Malignant histiocytosis: a specific t(2;5)(p23;q35) translocation? Review of the literature
- Author
-
Benz-Lemoine, E, primary, Brizard, A, additional, Huret, JL, additional, Babin, P, additional, Guilhot, F, additional, Couet, D, additional, and Tanzer, J, additional
- Published
- 1988
- Full Text
- View/download PDF
15. Aplasia cutis congenita of the scalp in an infant exposed to valproic acid in utero.
- Author
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Hubert, Armelle, Bonneau, Dominique, Couet, Dominique, Berthier, Michel, Oriot, Denis, Larregue, Marc, Hubert, A, Bonneau, D, Couet, D, Berthier, M, Oriot, D, and Larrègue, M
- Published
- 1994
- Full Text
- View/download PDF
16. Remote sensing of emperor penguin abundance and breeding success.
- Author
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Winterl A, Richter S, Houstin A, Barracho T, Boureau M, Cornec C, Couet D, Cristofari R, Eiselt C, Fabry B, Krellenstein A, Mark C, Mainka A, Ménard D, Morinay J, Pottier S, Schloesing E, Le Bohec C, and Zitterbart DP
- Subjects
- Animals, Breeding, Antarctic Regions, Seasons, Reproduction physiology, Population Density, Population Dynamics, Female, Spheniscidae physiology, Remote Sensing Technology methods
- Abstract
Emperor penguins (Aptenodytes forsteri) are under increasing environmental pressure. Monitoring colony size and population trends of this Antarctic seabird relies primarily on satellite imagery recorded near the end of the breeding season, when light conditions levels are sufficient to capture images, but colony occupancy is highly variable. To correct population estimates for this variability, we develop a phenological model that can predict the number of breeding pairs and fledging chicks, as well as key phenological events such as arrival, hatching and foraging times, from as few as six data points from a single season. The ability to extrapolate occupancy from sparse data makes the model particularly useful for monitoring remotely sensed animal colonies where ground-based population estimates are rare or unavailable., (© 2024. The Author(s).)
- Published
- 2024
- Full Text
- View/download PDF
17. Robotic pelvic and para-aortic retroperitoneal lymph nodes staging for early-stage epithelial ovarian cancer.
- Author
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Couet D, Tate B, Nguyen-Xuan HT, Koual M, Bats AS, Azais H, and Bentivegna E
- Subjects
- Female, Humans, Carcinoma, Ovarian Epithelial pathology, Lymph Nodes surgery, Lymph Nodes pathology, Neoplasm Staging, Lymph Node Excision, Retrospective Studies, Robotic Surgical Procedures, Ovarian Neoplasms surgery, Ovarian Neoplasms pathology
- Abstract
Competing Interests: Competing interests: EB reported being employed by Intuitive Surgical Inc (proctoring).
- Published
- 2023
- Full Text
- View/download PDF
18. Asherman Syndrome after Uterine Artery Embolization: A Cohort Study about Surgery Management and Fertility Outcomes.
- Author
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Jegaden M, Bleas C, Debras E, Couet D, Pourcelot AG, Capmas P, and Fernandez H
- Subjects
- Infant, Newborn, Female, Humans, Pregnancy, Adult, Cohort Studies, Retrospective Studies, Placenta, Hysteroscopy adverse effects, Fertility, Uterine Artery Embolization adverse effects, Postpartum Hemorrhage etiology, Postpartum Hemorrhage therapy, Gynatresia etiology, Gynatresia surgery, Uterine Diseases surgery, Uterine Diseases complications, Premature Birth
- Abstract
Study Objective: To study the severity of intrauterine adhesions (IUA) after uterine arterial embolization and to evaluate fertility, pregnancy, and obstetrical outcomes after hysteroscopic treatment., Design: Retrospective cohort., Setting: French University Hospital., Patients: Thirty-three patients under the age of 40 years who were treated by uterine artery embolization with nonabsorbable microparticles between 2010 and 2020 for symptomatic fibroids or adenomyosis, or postpartum hemorrhage., Interventions: All patients had a diagnosis of IUA after embolization. All patients desired future fertility. IUA was treated with operative hysteroscopy., Measurements and Main Results: Severity of IUA, number of operative hysteroscopies performed to obtain a normal cavity shape, pregnancy rate, and obstetrical outcomes. Of our 33 patients, 81.8% had severe IUA (state IV et V according to the European Society of Gynecological Endoscopy or state III according to the American fertility society classification). To restore fertility potential, an average of 3.4 operative hysteroscopies had to be performed [CI 95% (2.56-4.16)]. We reported a very low rate of pregnancy (8/33, 24%). Obstetrical outcomes reported are 50% of premature birth and 62.5% of delivery hemorrhage partly due to 37.5% of placenta accreta. We also reported 2 neonatal deaths., Conclusion: IUA after uterine embolization is severe, and more difficult to treat than other synechiae, probably related to endometrial necrosis. Pregnancy and obstetrical outcomes have shown a low pregnancy rate, an increased risk of preterm delivery, a high risk of placental disorders, and very severe postpartum hemorrhage. Those results have to alert gynecologists and radiologists to the use of uterine arterial embolization in women who desire future fertility., (Copyright © 2023 AAGL. Published by Elsevier Inc. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
19. Consequences of a contaminant mixture of bisphenol A (BPA) and di-(2-ethylhexyl) phthalate (DEHP), two plastic-derived chemicals, on the diversity of coastal phytoplankton.
- Author
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M'rabet C, Kéfi-Daly Yahia O, Couet D, Gueroun SKM, and Pringault O
- Subjects
- Benzhydryl Compounds analysis, Biodiversity, Chlorophyll A analysis, Diethylhexyl Phthalate analysis, Ecosystem, Phenols analysis, Phytoplankton growth & development, Seasons, Water Pollutants, Chemical analysis, Water Pollution, Chemical analysis, Benzhydryl Compounds pharmacology, Diethylhexyl Phthalate pharmacology, Environmental Biomarkers drug effects, Phenols pharmacology, Phytoplankton drug effects, Plastics chemistry, Water Pollutants, Chemical pharmacology
- Abstract
To assess the impact of two plastic derived chemicals: bisphenol A (BPA) and the di-2-ethylhexyl phthalate (DEHP), on phytoplankton biomass and community structure, microcosm incubations were performed during spring and summer, with offshore and lagoon waters of a south-western Mediterranean ecosystem. Phytoplankton were exposed to an artificial mixture of BPA and DEHP and to marine water previously enriched with plastic-derivative compounds, originated from in situ water incubations of plastic debris for 30 days. After 96 h of incubation, changes were observed in phytoplankton biomass in the contaminated microcosms, with a net decrease (up to 50% of the control) in the concentration of Chlorophyll a in offshore waters. Concomitantly, plastic-derivative contamination provoked structural changes, especially for offshore waters. This suggests a relative tolerance of the lagoon communities to BPA and DEHP contamination, related to the dominance of Chaetoceros spp., which could potentially be used as a bioindicator in bioassessment studies., (Copyright © 2018. Published by Elsevier Ltd.)
- Published
- 2019
- Full Text
- View/download PDF
20. Effects of copper and butyltin compounds on the growth, photosynthetic activity and toxin production of two HAB dinoflagellates: The planktonic Alexandrium catenella and the benthic Ostreopsis cf. ovata.
- Author
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Couet D, Pringault O, Bancon-Montigny C, Briant N, Elbaz Poulichet F, Delpoux S, Kefi-Daly Yahia O, Hela B, Charaf M, Hervé F, Rovillon G, Amzil Z, and Laabir M
- Subjects
- Biomass, Dinoflagellida growth & development, Dinoflagellida metabolism, Marine Toxins metabolism, Microalgae drug effects, Microalgae metabolism, Plankton drug effects, Plankton metabolism, Water Pollutants, Chemical chemistry, Copper toxicity, Dinoflagellida drug effects, Organotin Compounds toxicity, Photosynthesis drug effects, Water Pollutants, Chemical toxicity
- Abstract
Controlled laboratory experiments were conducted to test the effects of copper (Cu
2+ ) and butyltins (BuT) on the growth, photosynthetic activity and toxin content of two HABs (Harmful Algal Blooms) dinoflagellates, the planktonic Alexandrium catenella and the benthic Ostreopsis cf. ovata. Microalgae were exposed to increasing concentrations of Cu2+ (10-4 to 31 nM) or BuT (0.084 to 84 nM) for seven days. When considering the growth, EC50 values were 0.16 (±0.09) nM and 0.03 (±0.02) nM of Cu2+ for A. catenella and O. cf. ovata, respectively. Regarding BuT, EC50 was 14.2 (±6) nM for O. cf. ovata, while A. catenella growth inhibition appeared at BuT concentrations ≥27 nM. Photosynthetic activity of the studied dinoflagellates decreased with increasing Cu and BuT concentrations. For O. cf. ovata, the response of this physiological parameter to contamination was less sensitive than the biomass. Cu exposure induced the formation of temporary cysts in both organisms that could resist adverse conditions. The ovatoxin-a and -b concentrations in O. cf. ovata cells increased significantly in the presence of Cu. Altogether, the results suggest a better tolerance of the planktonic A. catenella to Cu and BuT. This could result in a differentiated selection pressure exerted by these metals on phytoplankton species in highly polluted waters. The over-production of toxins in response to Cu stress could pose supplementary health and socio-economic threats in the contaminated marine ecosystems where HABs develop., (Copyright © 2018 Elsevier B.V. All rights reserved.)- Published
- 2018
- Full Text
- View/download PDF
21. Impact of two plastic-derived chemicals, the Bisphenol A and the di-2-ethylhexyl phthalate, exposure on the marine toxic dinoflagellate Alexandrium pacificum.
- Author
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M'Rabet C, Pringault O, Zmerli-Triki H, Ben Gharbia H, Couet D, and Kéfi-Daly Yahia O
- Subjects
- Dinoflagellida growth & development, Dinoflagellida metabolism, Photosynthesis drug effects, Phthalic Acids, Toxicity Tests, Benzhydryl Compounds toxicity, Diethylhexyl Phthalate toxicity, Dinoflagellida drug effects, Phenols toxicity, Plastics toxicity, Water Pollutants, Chemical toxicity
- Abstract
The effects of two plastic-derived chemicals: Bisphenol A (BPA) and di-2-ethylhexyl phthalate (DEHP) were assessed on abundance and physiological responses of the marine toxic dinoflagellate Alexandrim pacificum. During 7days experiment, A. pacificum was exposed to different levels of BPA and DEHP (separately and in mixture). The responses were evaluated and compared with controls. Results showed that A. pacificum was highly sensitive to this contaminants comparing to other phytoplankton species. BPA and DEHP caused the decrease of the biomass (1.2 to 50 times lower relative to the controls), as well as the perturbation of the photosystem and the photosynthetic activity. Nevertheless, our results show a recovery of contaminated cells activity depending on exposure time and BPA and DEHP contamination. This could be related to an adaptation to induced stress or a degradation of BPA and DEHP in the medium., (Copyright © 2017 Elsevier Ltd. All rights reserved.)
- Published
- 2018
- Full Text
- View/download PDF
22. New insights on the species-specific allelopathic interactions between macrophytes and marine HAB dinoflagellates.
- Author
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Ben Gharbia H, Kéfi-Daly Yahia O, Cecchi P, Masseret E, Amzil Z, Herve F, Rovillon G, Nouri H, M'Rabet C, Couet D, Zmerli Triki H, and Laabir M
- Subjects
- Dinoflagellida classification, Dinoflagellida growth & development, Marine Toxins biosynthesis, Photosynthesis, Seaweed classification, Allelopathy, Dinoflagellida physiology, Marine Biology, Seaweed physiology
- Abstract
Macrophytes are known to release allelochemicals that have the ability to inhibit the proliferation of their competitors. Here, we investigated the effects of the fresh leaves of two magnoliophytes (Zostera noltei and Cymodocea nodosa) and thalli of the macroalgae Ulva rigida on three HAB-forming benthic dinoflagellates (Ostreopsis cf. ovata, Prorocentrum lima, and Coolia monotis). The effects of C. nodosa and U. rigida were also tested against the neurotoxic planktonic dinoflagellate Alexandrium pacificum Litaker sp. nov (former Alexandrium catenella). Co-culture experiments were conducted under controlled laboratory conditions and potential allelopathic effects of the macrophytes on the growth, photosynthesis and toxin production of the targeted dinoflagellates were evaluated. Results showed that U. rigida had the strongest algicidal effect and that the planktonic A. pacificum was the most vulnerable species. Benthic dinoflagellates seemed more tolerant to potential allelochemicals produced by macrophytes. Depending on the dinoflagellate/macrophyte pairs and the weight of leaves/thalli tested, the studied physiological processes were moderately to heavily altered. Our results suggest that the allelopathic activity of the macrophytes could influence the development of HAB species.
- Published
- 2017
- Full Text
- View/download PDF
23. Uptake of dissolved inorganic and organic nitrogen by the benthic toxic dinoflagellate Ostreopsis cf. ovata.
- Author
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Jauzein C, Couet D, Blasco T, and Lemée R
- Subjects
- Biological Transport, Dinoflagellida growth & development, Harmful Algal Bloom, Kinetics, Nitrogen Isotopes analysis, Nutrients, Dinoflagellida metabolism, Nitrogen metabolism
- Abstract
Environmental factors that shape dynamics of benthic toxic blooms are largely unknown. In particular, for the toxic dinoflagellate Ostreopsis cf. ovata, the importance of the availability of nutrients and the contribution of the inorganic and organic pools to growth need to be quantified in marine coastal environments. The present study aimed at characterizing N-uptake of dissolved inorganic and organic sources by O. cf. ovata cells, using the
15 N-labelling technique. Experiments were conducted taking into account potential interactions between nutrient uptake systems as well as variations with the diel cycle. Uptake abilities of O. cf. ovata were parameterized for ammonium (NH4 + ), nitrate (NO3 - ) and N-urea, from the estimation of kinetic and inhibition parameters. In the range of 0 to 10μmolNL-1 , kinetic curves showed a clear preference pattern following the ranking NH4 + >NO3 - >N-urea, where the preferential uptake of NH4 + relative to NO3 - was accentuated by an inhibitory effect of NH4 + concentration on NO3 - uptake capabilities. Conversely, under high nutrient concentrations, the preference for NH4 + relative to NO3 - was largely reduced, probably because of the existence of a low-affinity high capacity inducible NO3 - uptake system. Ability to take up nutrients in darkness could not be defined as a competitive advantage for O. cf. ovata. Species competitiveness can also be defined from nutrient uptake kinetic parameters. A strong affinity for NH4 + was observed for O. cf. ovata cells that may partly explain the success of this toxic species during the summer season in the Bay of Villefranche-sur-mer (France)., (Copyright © 2017 Elsevier B.V. All rights reserved.)- Published
- 2017
- Full Text
- View/download PDF
24. Association of structural and numerical anomalies of chromosome 22 in a patient with syndromic intellectual disability.
- Author
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Naoufal R, Legendre M, Couet D, Gilbert-Dussardier B, Kitzis A, Bilan F, and Harbuz R
- Subjects
- Adult, Chromosome Aberrations, Chromosome Deletion, Comparative Genomic Hybridization methods, Developmental Disabilities genetics, Humans, In Situ Hybridization, Fluorescence, Karyotyping, Language Development Disorders genetics, Male, Mosaicism, Chromosome Disorders genetics, Chromosomes, Human, Pair 22 genetics, Intellectual Disability genetics, Trisomy genetics, Uniparental Disomy genetics
- Abstract
Array comparative genomic hybridization (aCGH) is now widely adopted as a first-tier clinical diagnostic test for patients with developmental delay (DD)/intellectual disability (ID), autism spectrum disorders, and multiple congenital anomalies. Nevertheless, classic karyotyping still has its impact in diagnosing genetic diseases, particularly mosaic cases. We report on a 30 year old patient with syndromic intellectual disability, a 22q13.2 microdeletion and mosaic trisomy 22. The patient had the following clinical features: intrauterine growth retardation at birth, hypotonia, cryptorchidism, facial asymmetry, enophthalmus, mild prognathism, bifid uvula, hypoplastic upper limb phalanges, DD including speech delay, and ID. Whole genome aCGH showed a de novo 1 Mb interstitial heterozygous deletion in 22q13.2, confirmed by fluorescence in situ hybridization in all cells examined. Moreover, 18% cells had an extra chromosome 22 suggesting a trisomy 22 mosaicism. Almost all 22q13 deletions published so far have been terminal deletions with variable sizes (100 kb to over 9 Mb). Very few cases of interstitial 22q13.2 deletions were reported. In its mosaic form, trisomy 22 is compatible with life, and there are about 20 reports in the literature. It has a variable clinical presentation: growth restriction, dysmorphic features, cardiovascular abnormalities, hemihyperplasia, genitourinary tract anomalies and ID. Neurodevelopmental outcome ranges from normal to severe DD. The patient presents clinical features that are common to both the interstitial 22q13 deletion and the mosaic trisomy 22; characteristics related to the interstitial deletion alone and others explained solely by the mosaic trisomy. Our case points out the role of conventional cytogenetic tools in mosaic cases that could be missed by microarray technology. We therefore suggest the combination of both conventional and molecular karyotyping in the investigation of certain genetic diseases., (Copyright © 2016 Elsevier Masson SAS. All rights reserved.)
- Published
- 2016
- Full Text
- View/download PDF
25. Osteogenesis imperfecta, tricho-dento-osseous syndrome and intellectual disability: a familial case with 17q21.33-q22 (COL1A1 and DLX3) deletion and 7q32.3-q33 duplication resulting from a reciprocal interchromosomal insertion.
- Author
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Harbuz R, Bilan F, Couet D, Charraud V, Kitzis A, and Gilbert-Dussardier B
- Subjects
- Chromosome Duplication, Chromosomes, Human, Pair 17, Chromosomes, Human, Pair 7, Collagen Type I genetics, Collagen Type I, alpha 1 Chain, Comparative Genomic Hybridization, Craniofacial Abnormalities diagnosis, Dental Enamel Hypoplasia diagnosis, Female, Hair Diseases diagnosis, Homeodomain Proteins genetics, Humans, In Situ Hybridization, Fluorescence, Intellectual Disability diagnosis, Male, Middle Aged, Mutagenesis, Insertional, Osteogenesis Imperfecta diagnosis, Pedigree, Phenotype, Sequence Deletion, Syndrome, Transcription Factors genetics, Young Adult, Craniofacial Abnormalities genetics, Dental Enamel Hypoplasia genetics, Hair Diseases genetics, Intellectual Disability genetics, Osteogenesis Imperfecta genetics
- Abstract
We report on a 22-year-old woman with features of osteogenesis imperfecta (OI), tricho-dento-osseous (TDO) syndrome and intellectual disability. Whole genome oligonucleotide microarray analysis revealed a copy number gain of 3 Mb in 7q32.3-q33 and a loss of 3.4 Mb in 17q21.33-q22. FISH analysis showed that the third copy of 7q32 was inserted into the long arm of one chromosome 17, exactly in the region 17q21.33-q22 that was deleted. The maternal uncle presented with clinical features similar to the proposita and had the same chromosomal anomalies. The mother of the proposita and two other family members were balanced carriers of this rearrangement, interpreted as an interchromosomal reciprocal insertion. Reciprocal insertion/four-break rearrangement is a very rare chromosomal event. The deleted region on chromosome 17 contains 39 genes, including COL1A1 and DLX3 involved in OI and TDO syndrome respectively. The CACNA1G gene on the deleted segment of chromosome 17 may be a good candidate gene to explain the intellectual impairment. © 2013 Wiley Periodicals, Inc., (Copyright © 2013 Wiley Periodicals, Inc.)
- Published
- 2013
- Full Text
- View/download PDF
26. Complete screening of 50 patients with CHARGE syndrome for anomalies in the CHD7 gene using a denaturing high-performance liquid chromatography-based protocol: new guidelines and a proposal for routine diagnosis.
- Author
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Bilan F, Legendre M, Charraud V, Manière B, Couet D, Gilbert-Dussardier B, and Kitzis A
- Subjects
- CHARGE Syndrome genetics, Computer Simulation, DNA Helicases, DNA-Binding Proteins, Exons, Gene Dosage, Gene Rearrangement, Humans, In Situ Hybridization, Fluorescence, Karyotyping, Models, Genetic, Mutation, Practice Guidelines as Topic, CHARGE Syndrome diagnosis, Chromatography, High Pressure Liquid methods, DNA Mutational Analysis methods, Genetic Testing methods
- Abstract
Ocular coloboma, heart malformation, choanal atresia, retardation of growth and/or development, genital hypoplasia, and ear anomalies associated with deafness (CHARGE) syndrome is a rare, usually sporadic, autosomal dominant disorder, caused by mutations within the CHD7 (chromodomain helicase DNA-binding protein 7) gene, in nearly 70% of cases. Because human CHD7 is relatively large (38 exons encoding a 300-kDa protein), genetic analysis requires cost-effective and time-consuming techniques. Herein, we propose an alternative screening method to quickly detect CHD7 mutations using mainly denaturing high-performance liquid chromatography. The entire coding region with exon-intron boundaries was amplified under the same experimental conditions. Each amplicon of the same CHD7 region was subjected to denaturing high-performance liquid chromatography analysis, and resulting chromatograms were compared within small series of patients. Because a CHD7 mutation differs generally from one patient to another, corresponding chromatograms exhibited a unique pattern that is significantly different from common polymorphisms. Only amplicons exhibiting a unique profile were subjected to DNA sequencing analysis. Intragenic rearrangements were investigated with only nine multiplex PCRs. In conclusion, using our protocol, we can quickly detect the right containing mutation amplicon and we provide a robust, rapid, and cheaper method to screen CHD7 microrearrangements or an entire deletion., (Copyright © 2012 American Society for Investigative Pathology and the Association for Molecular Pathology. Published by Elsevier Inc. All rights reserved.)
- Published
- 2012
- Full Text
- View/download PDF
27. [Molecular biology usefulness for rapid diagnosis of Down's syndrome and common aneuploidies].
- Author
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Fauret AL, Bilan F, Patri S, Couet D, Marechaud M, Pierre F, Gilbert-Dussardier B, and Kitzis A
- Subjects
- Chorionic Villi Sampling, Chromosome Aberrations, Female, Humans, Karyotyping, Maternal Age, Microsatellite Repeats, Nuchal Translucency Measurement, Predictive Value of Tests, Pregnancy, Prenatal Diagnosis standards, Reproducibility of Results, Sensitivity and Specificity, Time Factors, Ultrasonography, Prenatal standards, Aneuploidy, Down Syndrome diagnosis, Prenatal Diagnosis methods, Ultrasonography, Prenatal methods
- Abstract
Objective: Trisomy of chromosome 13, 18, 21 and sex chromosome aneuploidies are the most common chromosomal abnormalities encountered in prenatal screening and are responsible for polymaformative syndrome associated with severe mental retardation. This high degree of morbidity justifies the prenatal diagnosis of these aneuploidies. Fetal nuchal translucency measurement and maternal serum biochemical marker assessment are the method of choice used for antenatal screening of aneuploidies. This prenatal screening leads to numerous maternal samplings followed by karyotyping which is cost-effective, time consuming, while results are generally returned between 2 and 3 weeks. Our study describes the research of common aneuploidies by molecular biology. We have used on one hand the MLPA kit (MRC Holland) based on amplification of specific DNA probes that hybridize with chromosomes 13, 18, 21, X, Y. On the other hand we have developed multiplex fluorescent PCR, amplifying microsatellite DNA sequences., Patients and Methods: We have evaluated the efficiency of these two techniques to detect chromosomal abnormalities by screening 400 amniotic fluids or chorionic villi samples obtained from pregnant women presenting a high risk of chromosomal aneuploidy., Results: We have found four trisomies 21, one trisomy 13, one monosomy 13, one trisomy 18, two triploidies, one trisomy X and one Klinefelter syndrome., Discussion and Conclusion: In our study we have detected by molecular biology, in less than 48 h, 100% of common chromosomal aneuploidies without false positive or false negative results which could lead molecular biology as a method of choice for the rapid detection of common aneuploidies in addition to fetal karyotyping.
- Published
- 2009
- Full Text
- View/download PDF
28. [Williams-Beuren syndrome: a multidisciplinary approach].
- Author
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Lacroix A, Pezet M, Capel A, Bonnet D, Hennequin M, Jacob MP, Bricca G, Couet D, Faury G, Bernicot J, and Gilbert-Dussardier B
- Subjects
- Abnormalities, Multiple genetics, Chromosome Deletion, Genetic Testing, Humans, Intellectual Disability genetics, Williams Syndrome genetics
- Abstract
Williams-Beuren syndrome (WBS) (OMIM# 194050) is a rare, most often sporadic, genetic disease caused by a chromosomal microdeletion at locus 7q11.23 involving 28 genes. Among these, the elastin gene codes for the essential component of the arterial extracellular matrix. Developmental disorders usually associate an atypical face, cardiovascular malformations (most often supravalvular aortic stenosis and/or pulmonary artery stenosis) and a unique neuropsychological profile. This profile is defined by moderate mental retardation, relatively well-preserved language skills, visuospatial deficits and hypersociability. Other less known or rarer features, such as neonatal hypercalcemia, nutrition problems in infancy, ophthalmological anomalies, hypothyroidism, growth retardation, joint disturbances, dental anomalies and hypertension arising in adolescence or adulthood, should be treated. The aim of this paper is to summarize the major points of WBS regarding: (i) the different genes involved in the deletion and their function, especially the elastin gene and recent reports of rare forms of partial WBS or of an opposite syndrome stemming from a microduplication of the 7q11.23 locus, (ii) the clinical features in children and adults with a focus on cardiovascular injury, and (iii) the specific neuropsychological profile of people with WBS through its characteristics, the brain structures involved, and learning.
- Published
- 2009
- Full Text
- View/download PDF
29. [A breath for fresh air...with respect to a dictionary for medical thoughts].
- Author
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Couet D
- Subjects
- Communication, Humans, Public Health, Terminology as Topic
- Published
- 2005
- Full Text
- View/download PDF
30. Obesity: a new feature of WAGR (del 11p) syndrome.
- Author
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Marlin S, Couet D, Lacombe D, Cessans C, and Bonneau D
- Subjects
- Aniridia, Cataract, Child, Developmental Disabilities, Female, Humans, WAGR Syndrome diagnosis, Chromosome Deletion, Chromosomes, Human, Pair 11, Obesity, WAGR Syndrome classification
- Abstract
A 6-year-old girl with del(11)(p14p12) is reported. This girl has the multiple congenital anomalies that defines the WAGR syndrome (aniridia, external genital hypoplasia and severe mental retardation). She has, in addition, very severe obesity (+10 SD) which is not a feature usually described with WAGR association.
- Published
- 1994
31. Lujan-Fryns syndrome (X-linked mental retardation with marfanoid habitus): report of three cases and review.
- Author
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Lacombe D, Bonneau D, Verloes A, Couet D, Koulischer L, and Battin J
- Subjects
- Abnormalities, Multiple genetics, Adolescent, Arm abnormalities, Facial Bones abnormalities, Foot Deformities, Congenital pathology, Hand Deformities, Congenital pathology, Humans, Male, Marfan Syndrome, Skull abnormalities, Syndrome, Abnormalities, Multiple pathology, Genetic Linkage, Growth Disorders pathology, Intellectual Disability genetics, X Chromosome
- Abstract
We report on three further cases of mildly retarded patients with marfanoid habitus and a pattern of minor anomalies. These patients are likely to be affected with the Lujan-Fryns syndrome. We have reviewed twenty cases from the literature for a better delineation of this newly recognized disorder.
- Published
- 1993
32. INV(12) (p13q11) and dicentric chromosomes in a secondary leukaemia with basophilia.
- Author
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Huret JL, Brizard A, Guilhot F, Couet D, Schoenwald M, Savage JR, and Tanzer J
- Subjects
- Aged, Antineoplastic Combined Chemotherapy Protocols adverse effects, Blast Crisis genetics, Chromosome Inversion, Dacarbazine administration & dosage, Dacarbazine adverse effects, Female, Humans, Leukemia, Myelomonocytic, Chronic chemically induced, Leukemia, Myelomonocytic, Chronic pathology, Melanoma drug therapy, Methotrexate administration & dosage, Methotrexate adverse effects, Skin Neoplasms drug therapy, Streptonigrin administration & dosage, Streptonigrin adverse effects, Thiotepa administration & dosage, Thiotepa adverse effects, Vinblastine administration & dosage, Vinblastine adverse effects, Basophils pathology, Chromosome Aberrations, Chromosomes, Human, Pair 12 ultrastructure, Chromosomes, Human, Pair 5 ultrastructure, Chromosomes, Human, Pair 6 ultrastructure, Chromosomes, Human, Pair 8 ultrastructure, Leukemia, Myelomonocytic, Chronic genetics, Neoplasms, Multiple Primary
- Abstract
We report on a case of secondary myelodysplasia with basophilia. A karyotype showed 1) the involvement of 12p, which should be related to the basophilia and/or to previous drug exposure, and 2) the unexpected presence of three different dicentric chromosomes.
- Published
- 1990
33. Education on sexuality, the family and society today.
- Author
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Couet D and Lecorps P
- Subjects
- Africa, Africa South of the Sahara, Africa, Northern, Africa, Western, Burkina Faso, Developing Countries, Family Planning Services, Health, Health Planning, Research, Community Health Workers, Delivery of Health Care, Education, Health Education, Health Personnel, Models, Theoretical, Sex Education, Teaching
- Published
- 1985
34. Aberrant translocations in chronic myelogenous leukaemia with hidden chromosome 9 or 22 involvement.
- Author
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Huret JL, Bresson JL, Couet D, and Tanzer J
- Subjects
- Adult, Aged, Female, Humans, Male, Chromosomes, Human, Pair 22, Chromosomes, Human, Pair 9, Leukemia, Myeloid genetics, Translocation, Genetic
- Abstract
Possible mechanisms are proposed to explain how chromosome 9 or 22 involvement can be hidden in aberrant translocations in chronic myelogenous leukaemia. These mechanisms need to be confirmed or rejected using in situ hybridization.
- Published
- 1987
35. A two-step t(4;der(15)) t(15;17) complex translocation in an acute promyelocytic leukaemia and review of the literature.
- Author
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Huret JL, Couet D, Guilhot F, Brizard A, and Tanzer J
- Subjects
- Aged, Chromosomes, Human, Pair 15, Chromosomes, Human, Pair 17, Chromosomes, Human, Pair 4, Humans, Karyotyping, Male, Leukemia, Myeloid, Acute genetics, Translocation, Genetic
- Published
- 1987
- Full Text
- View/download PDF
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