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One of the main goals of building design is indoor comfort, regardless of its use (residential, educational, institutional, etc…). However, to achieve indoor comfort, buildings require a significant amount of energy. In the last decades, designers and researchers have been studying new strategies to improve buildings’ energy efficiency, with the purpose of mitigating the negative environmental impact caused by heavy energy consumption. Green roofs have been one of the most investigated solutions because of the many thermal benefits they can offer, and amongst these, hydroponic green roofs gained momentum. This study aims to analyse the rooftop temperature reduction provided during the hot months by a hydroponic green roof, compared to a traditional roof slab and an extensive green roof, in order to assess the different performances of these systems. In situ experiments were conducted to collect surface temperature of the roof slab during summer, with and without the hydroponic system, in order to assess the potential temperature reduction, which subsequently affects the heat flow through the roof and therefore the indoor air temperature. The results show a significant decrease in the external surface temperature of the roof compared to the bare roof, but also slightly better performance compared to the extensive green roof. Despite first promising results, the knowledge on hydroponic green roofs performance remains limited and some drawbacks need to be assessed. For these reasons, further in situ testing should be carried out, under different climatic conditions and experimental setups.

Error in Figure In the original publication [1], there was a mistake in Figure 2 as published. The figure reported a representative image of the renal cortex acquired in vivo by two-photon microscopy for other purposes than this review. Consequently, the image was removed and the figure legend was modified. The corrected Figure 2 and its legend appear below:.

Introduction. The international literature widely agrees that one of the most serious problems of health services is elderly patients readmission, usually 30 days after their discharge. While many studies identified disease variables related to readmission, only few studies investigated the relationship between nursing-related variables and readmission. Aim. To identify the association between nursing care needs at the discharge of elderly patients and hospital readmissions at 30 days. Methods. An observational longitudinal study was conducted enrolling a sample of 148 patients aged over 65. Nursing needs were identified by and Italian theoretical model and the International Classification for Nursing Practice. Data were collected by medical records. Linear correlations and logistic regression models were performed. Results. Readmission at 30 days was 19.6%. Nursing needs of Safe environment (p = 0.04) and Communication (p = 0.06) were significantly associated to 30-days readmission. Furthermore, an higher risk for readmission was found for those patients who had at least one of the following ICNP nursing diagnoses: Impaired psychomotor activity, Aggressive behaviors, Chronic confusion, Risk for confusion, Risk for delirium and Agitation (p = 0.009). Conclusions. The need for nursing care as well as some specific ICNP nursing diagnoses represent significant risk factors for the readmission of elderly patients. These needs and diagnoses should be systematically evaluated to plan patients discharge. Further studies are needed to evaluate on a larger international sample ICNP nursing diagnoses associated to 30-days readmission of hospitalized older people

Controversies exist about the definition and epidemiology of breakthrough cancer pain (BTcP), the pharmacological treatment options, drug dosing, and how to select the medications for BTcP among the new fentanyl products. Existing data were critically evaluated to provide recommendations by an expert group. An algorithm to diagnose BTcP should be used followed by a careful assessment. Fentanyl products provide efficacy and rapidity of action to counteract the temporal pattern of BTcP. The doses of opioids used for background pain should guide the choice of the doses of fentanyl products. The choice of fentanyl products should be based on individual clinical conditions.

Camptothecin-induced locking of topoisomerase 1 on DNA generates a physical barrier to replication fork progression and creates topological stress. By allowing replisome rotation, absence of the Tof1/Csm3 complex promotes the conversion of impending topological stress to DNA catenation and causes camptothecin hypersensitivity. Through synthetic viability screening, we discovered that histone H4 K16 deacetylation drives the sensitivity of yeast cells to camptothecin and that inactivation of this pathway by mutating H4 K16 or the genes $\textit{SIR1-4}$ suppresses much of the hypersensitivity of $\textit{tof1}$∆ strains towards this agent. We show that disruption of rDNA or telomeric silencing does not mediate camptothecin resistance but that disruption of Sir1-dependent chromatin domains is sufficient to suppress camptothecin sensitivity in wild-type and $\textit{tof1}$∆ cells. We suggest that topoisomerase 1 inhibition in proximity of these domains causes topological stress that leads to DNA hypercatenation, especially in the absence of the Tof1/Csm3 complex. Finally, we provide evidence of the evolutionarily conservation of this mechanism.

The centromere is the genetic locus that specifies the site of kinetochore assembly, where the chromosome will attach to the kinetochore microtubule. The pericentromere is the physical region responsible for the geometry of bi-oriented sister kinetochores in metaphase. In budding yeast the 125 bp point centromere is sufficient to specify kinetochore assembly. The flanking region is enriched (3X) in cohesin and condensin relative to the remaining chromosome arms. The enrichment spans about 30-50 kb around each centromere. We refer to the flanking chromatin as the pericentromere in yeast. In mammals, a 5-10 Mb region dictates where the kinetochore is built. The kinetochore interacts with a very small fraction of DNA on the surface of the centromeric region. The remainder of the centromere lies between the sister kinetochores. This is typically called centromere chromatin. The chromatin sites that directly interface to microtubules cannot be identified due to the repeated sequence within the mammalian centromere. However in both yeast and mammals, the total amount of DNA between the sites of microtubule attachment in metaphase is highly conserved. In yeast the 16 chromosomes are clustered into a 250 nm diameter region, and 800 kb (16 × 50 kb) or ~1 Mb of DNA lies between sister kinetochores. In mammals, 5-10 Mb lies between sister kinetochores. In both organisms the sister kinetochores are separated by about 1 μm. Thus, centromeres of different organisms differ in how they specify kinetochore assembly, but there may be important centromere chromatin functions that are conserved throughout phylogeny. Recently, centromeric chromatin has been reconstituted in vitro using alpha satellite DNA revealing unexpected features of centromeric DNA organization, replication, and response to stress. We will focus on the conserved features of centromere in this review.