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Introduction: We studied the release of propranolol hydrochloride (PHCl), a water-soluble amphiphilic drug, from monoolein (MO)/water and phytantriol/water systems., Methods: We related the dissolution profiles with phase behavior and viscosity of the different liquid crystalline phases. Diolein has been added aiming to stabilize the cubic phases and thus preventing formation of less viscous (lamellar) phases., Results: Formulations display first-order release rates and diffusion release mechanism. Some formulations (mostly MO) were close to zero-order release in the first 120 minutes., Discussion: Release mechanism can be influenced by phase changes during dissolution., Conclusions: Both MO and phytantriol show good potential to be used for propranolol hydrochloride sustained drug release.

The large osmotic gradient over the outermost layer of human skin implies major structural changes along the gradient, which in turn affects transport. In particular, the possibility of phase changes introduces a non-linear element to the transport behaviour. We present a novel model membrane system to be used for studying these transport mechanisms, where we use a hydrophobic porous polymer membrane as a scaffold for lipid lyotropic phases. The polymer membrane provides mechanical robustness, but also prevents defects of the lipid lyotropic phases, and it can induce an orientation of anisotropic phases. We study the location, structure and phase behaviour of the confined phases. It is shown that this model membrane system allow for accurate measurements of transport through lipid membranes in the presence of different osmotic gradients. A theoretical description is evaluated and shows that this phenomenon can be understood in terms of the proposed mechanism of phase changes. The novel double-porous lipid membrane constitutes a mechanically robust system for studies in aligned systems, which is generally very difficult to achieve. This could have large implications for studies of transport processes in, e.g. skin and other biomembrane model systems.

We investigate how a small polar molecule, urea, can act to protect a phospholipid bilayer system against osmotic stress. Osmotic stress can be caused by a dry environment, by freezing, or by exposure to aqueous systems with high osmotic pressure due to solutes like in saline water. A large number of organisms regularly experience osmotic stress, and it is a common response to produce small polar molecules intracellularly. We have selected a ternary system of urea-water-dimyristoyl phosphatidylcholine (DMPC) as a model to investigate the molecular mechanism behind this protective effect, in this case, of urea, and we put special emphasis on the applications of urea in skin care products. Using differential scanning calorimetry, X-ray diffraction, and sorption microbalance measurements, we studied the phase behavior of lipid systems exposed to an excess of solvent of varying compositions, as well as lipid systems exposed to water at reduced relative humidities. From this, we have arrived at a rather detailed thermodynamic characterization. The basic findings are as follows: (i) In excess solvent, the thermally induced lipid phase transitions are only marginally dependent on the urea content, with the exception being that the P(beta) phase is not observed in the presence of urea. (ii) For lipid systems with limited access to solvent, the phase behavior is basically determined by the amount (volume) of solvent irrespective of the urea content. (iii) The presence of urea has the effect of retaining the liquid crystalline phase at relative humidities down to 64% (at 27 degrees C), whereas, in the absence of urea, the transition to the gel phase occurs already at a relative humidity of 94%. This demonstrates the protective effect of urea against osmotic stress. (iv) In skin care products, urea is referred to as a moisturizer, which we find slightly misleading as it replaces the water while keeping the physical properties unaltered. (v) In other systems, urea is known to weaken the hydrophobic interactions, while for the lipid system we find few signs of this loosening of the strong segregation into polar and apolar regions on addition of urea.